CN215799548U - Card box with quantitative measuring structure - Google Patents
Card box with quantitative measuring structure Download PDFInfo
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- CN215799548U CN215799548U CN202121112550.4U CN202121112550U CN215799548U CN 215799548 U CN215799548 U CN 215799548U CN 202121112550 U CN202121112550 U CN 202121112550U CN 215799548 U CN215799548 U CN 215799548U
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Abstract
The utility model relates to the technical field of biomedical treatment, and discloses a card box with a quantitative measuring structure, which comprises a card box body and at least one group of quantitative structure units arranged on the card box body, wherein each quantitative structure unit comprises a first cavity, a second cavity, a first connecting flow channel, a second connecting flow channel, a first valve, a second valve, a quantitative cavity and a quantitative cavity air passage; the first cavity is communicated with the bottom of the quantitative cavity through a first connecting flow channel, and a first valve is arranged on the first connecting flow channel; the second chamber is communicated with the bottom of the quantitative cavity through a second connecting flow channel, and the second valve is arranged on the second connecting flow channel; one end of the quantitative cavity air passage is provided with an air vent, and the other end of the quantitative cavity air passage is arranged at the top of the quantitative cavity. The quantitative liquid measuring device can measure quantitative liquid from one chamber to the other chamber, is simple to operate and convenient for automatic control, does not need manual operation in the quantitative measuring process, and effectively avoids the pollution of the test liquid in the measuring process, thereby being beneficial to improving the detection accuracy.
Description
Technical Field
The utility model relates to the technical field of biomedical treatment, in particular to a card box with a quantitative measuring structure.
Background
Nucleic acid detection plays a very important role in many fields of biochemical analysis, and has been widely applied in the field of biomedical science.
In the prior art, nucleic acid extraction is generally carried out by using a centrifugal column method or a magnetic bead method, and four steps of lysis, combination, washing, elution and the like are generally required. After the nucleic acid extraction is carried out on the sample, the subsequent steps of nucleic acid molecular hybridization, Polymerase Chain Reaction (PCR), biochip and the like are carried out continuously to complete the nucleic acid detection.
In the above detection steps, some steps need to be performed by using a quantitative test solution, but the detection device in the prior art is difficult to measure a quantitative test solution from one chamber and transfer the quantitative test solution to another chamber without manual operation due to the structural limitations of the chambers and the communication flow channel.
Thus, improvements in the prior art are needed.
SUMMERY OF THE UTILITY MODEL
The purpose of the utility model is: the utility model provides a card box with a quantitative measuring structure, which aims to solve the technical problem that in the detection equipment in the prior art, due to the structural limitation of a chamber and a communicating flow channel, quantitative test solution is difficult to be measured from one chamber and transferred to the other chamber without manual operation.
In order to achieve the above object, the present invention provides a cartridge, comprising a cartridge body and at least one set of quantitative structure unit arranged on the cartridge body, wherein the quantitative structure unit comprises a first chamber, a second chamber, a first connecting flow channel, a second connecting flow channel, a first valve, a second valve, a quantitative cavity and a quantitative cavity air channel;
the first cavity is communicated with the bottom of the quantitative cavity through the first connecting flow channel, and the first valve is arranged on the first connecting flow channel;
the second chamber is communicated with the bottom of the quantitative cavity through the second connecting flow channel, and the second valve is arranged on the second connecting flow channel;
one end of the quantitative cavity air passage is provided with an air vent, and the other end of the quantitative cavity air passage is arranged at the top of the quantitative cavity.
In some embodiments of the present application, a transition flow channel is disposed at a bottom centerline of the quantitative cavity, and the first connection flow channel and the second connection flow channel are respectively communicated with the transition flow channel.
In some embodiments of the present application, the first chamber is provided with a first airway having a first port.
In some embodiments of the present application, the first connection flow channel communicates with the bottom of the first chamber, and the first air channel is disposed at the top of the first chamber.
In some embodiments of the present application, the second chamber is provided with a second airway having a second port.
In some embodiments of the present application, the second connection flow passage is communicated with a bottom of the second chamber, and the second air passage is disposed at a top of the second chamber.
In some embodiments of the present application, the first valve and the second valve are needle lift valves.
In some embodiments of the present application, the first valve has a passage therein, and a sealing film is provided on the passage, and the sealing film deforms when subjected to a predetermined pressure, thereby closing the passage.
In some embodiments of the present application, the bottom of the dosing chamber is funnel-shaped.
In some embodiments of the present application, the cartridge body comprises a cartridge plate and a front sealing membrane;
the surface of the card box plate is provided with a plurality of grooves, and the front sealing film is pasted on the surface of the card box plate to seal the grooves so as to form a first cavity, a second cavity, a first connecting flow channel, a second connecting flow channel, a quantitative cavity and a quantitative cavity air channel.
Compared with the prior art, the card box with the quantitative measuring structure has the advantages that:
according to the card box with the quantitative measuring structure, the quantitative cavity is connected to the flow channel between the two cavities, the valves are arranged on two sides of the connecting position, the air passage of the quantitative cavity is formed in the top of the quantitative cavity, the air pump can be connected to the air passage of the quantitative cavity, the air pump can be used for controlling the flow direction of liquid, and the quantitative cavity can measure quantitative test liquid from one cavity and then push the test liquid into the other cavity. The quantitative measuring device is simple to operate and convenient to automatically control, the quantitative measuring process does not need manual operation at all, and the test solution is effectively prevented from being polluted in the measuring process, so that the detection accuracy rate is improved.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed for the embodiments or the prior art descriptions will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.
FIG. 1 is a schematic front view of a cartridge body according to an embodiment of the present invention;
FIG. 2 is a schematic diagram of the structure of a first set of quantitative building blocks;
FIG. 3 is a schematic structural view of a second group of quantitative building blocks;
FIG. 4 is a schematic view of the structure of the needle top valve in the open state;
FIG. 5 is a schematic view of the structure of the needle top valve in the closed state;
FIG. 6 is a schematic view of an exploded structure of a cartridge of an embodiment of the utility model;
in the figure, 1, a cartridge body; 11. a cassette plate; 12. front sealing the film;
2. a quantitative structural unit; 200. a dosing chamber; 210. a dosing chamber airway; 211. a vent; 212. a transition flow channel; 221. a first connecting flow passage; 222. a second connecting flow channel; 231. a first valve; 232. a second valve; 241. a first chamber; 242. a second chamber; 251. a first air passage; 252. a second air passage; 261. a first gas port; 262. a second gas port;
3. a sealing film; 4. a drive rod.
Detailed Description
The following detailed description of embodiments of the present invention is provided in connection with the accompanying drawings and examples. The following examples are intended to illustrate the utility model but are not intended to limit the scope of the utility model.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "left", "right", "top", "bottom", and the like, indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience in describing the present invention and simplifying the description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention.
It will be understood that the terms "length," "width," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like, as used herein, refer to an orientation or positional relationship indicated in the drawings, which is solely for the purpose of facilitating the description and simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and is therefore not to be construed as limiting the utility model.
Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.
Referring to fig. 1, the cartridge with a quantitative measuring structure according to the present invention includes a cartridge body 1 and at least one set of quantitative structure unit 2 disposed on the cartridge body 1.
Specifically, referring to fig. 2 and 3, the quantitative structure unit 2 includes a first chamber 241, a second chamber 242, a first connection flow channel 221, a second connection flow channel 222, a first valve 231, a second valve 232, a quantitative cavity 200, and a quantitative cavity air channel 210.
The first chamber 241 is connected to the bottom of the quantitative cavity 200 through a first connecting channel 221, and a first valve 231 is disposed on the first connecting channel 221. The second chamber 242 is communicated with the bottom of the quantitative cavity 200 through a second connection flow passage 222, and a second valve 232 is provided on the second connection flow passage 222. The quantitative chamber air passage 210 has a vent 211 at one end thereof, the vent 211 being communicated with an external air pump, and the other end thereof being disposed at the top of the quantitative chamber 200.
The cartridge with a quantitative measuring structure provided by the utility model can measure a certain amount of test solution from the first chamber 241 to the second chamber 242 through the quantitative structure unit 2, and the measuring process is as follows:
in the first step, the vent 211 of the fixed-volume air passage 210 is communicated with an external air pump.
In the second step, the first valve 231 is opened and the second valve 232 is closed.
Thirdly, the external air pump sucks air from the quantitative cavity 200 through the quantitative cavity air passage 210, so that the test solution in the first cavity 241 is sucked into the quantitative cavity 200 along the first connecting flow passage 221, the sucked amount can be detected by a quantitative detection module of a matched instrument, and when the liquid amount reaches the required amount, the air suction is stopped.
In the fourth step, the first valve 231 is closed and the second valve 232 is opened.
Fifthly, the external air pump vents the quantitative cavity 200 through the quantitative cavity air passage 210, pushes the quantitative test solution in the quantitative cavity 200 into the second cavity 242 along the second connecting flow passage 222, and then closes the second valve 232 to finish measuring.
Specifically, the quantitative detection module of supporting instrument can use high definition camera, carries out real time monitoring to the liquid level and shoots the back and carry out intelligent recognition to the liquid level to detect intracavity liquid volume. Or, using a pressure sensor, monitoring the pressure in the cavity and judging the liquid amount in the cavity according to a pressure model.
The card box with the quantitative measuring structure, provided by the utility model, is characterized in that a quantitative cavity 200 is connected to a flow channel between two cavities on a card box body, valves are arranged on two sides of the connection position, and a quantitative cavity air channel 210 arranged on the top of the quantitative cavity 200 can be connected to an air pump for controlling the flow direction of liquid, so that the quantitative cavity 200 can measure quantitative test liquid from one cavity and then push the test liquid into the other cavity. The quantitative measuring device is simple to operate and convenient to automatically control, the quantitative measuring process does not need manual operation at all, and the test solution is effectively prevented from being polluted in the measuring process, so that the detection accuracy rate is improved.
In some embodiments of the present application, referring to fig. 2 and 3, a transition flow channel 212 is disposed at a central line of a bottom of the quantitative cavity 200, the first connection flow channel 221 and the second connection flow channel 222 are respectively communicated with the transition flow channel 212, so that the first chamber 241 and the second chamber 242 are respectively located at two sides of the quantitative cavity 200, and liquid enters and exits the quantitative cavity 200 through the transition flow channel 212.
In some embodiments of the present application, referring to fig. 2 and 3, the first chamber 241 is provided with a first air channel 251, the first air channel 251 has a first air port 261, and the first air port 261 is communicated with an external air pump.
Further, the first connecting channel 221 is communicated with the bottom of the first chamber 241, and the first air channel 251 is disposed at the top of the first chamber 241.
In the above embodiment, after the first air channel 251 is disposed in the first chamber 241, another operation mode may be provided to transfer the sample solution to the quantitative cavity 200 in the third step. That is, the external air pump may ventilate the first chamber 241 through the first air channel 251, push the sample solution in the first chamber 241 into the quantitative cavity 200 along the first connection flow channel 221, detect the amount of the solution measured in the cavity through the quantitative detection module of the kit, and stop ventilating through the first air channel 251 when the amount of the solution reaches a desired amount.
In some embodiments of the present application, referring to fig. 2 and 3, the second chamber 241 is provided with a second air passage 252, and the second air passage 252 has a second air port 262.
Further, the second connection flow passage 222 is communicated with the bottom of the second chamber 242, and the second air passage 252 is disposed at the top of the second chamber 242.
In the above embodiment, the second air passage 252 is disposed in the second chamber 241, so that the test solution in the second chamber 241 can be conveniently pushed into the reaction chamber of the next step.
In some embodiments of the present application, the first valve 231 and the second valve 232 are both needle-lift valves. In the prior art, a structure of a needle valve for controlling liquid is available, and the needle valve can be applied to the cartridge of the present application as the first valve 231 and the second valve 232 through size adaptation.
In some embodiments of the present application, a preferred thimble valve structure is provided. Referring to fig. 4 and 5, the first valve 231 has a passage therein, and the sealing film 3 is disposed on the passage, and the sealing film 3 is deformed by a predetermined pressure to close the passage, so that the needle valve is closed. When the thimble valve is actually used, the thimble valve can be matched with a driving rod 4 of a matched instrument, and when the driving rod 4 moves forwards to press the sealing film 3, the thimble valve is closed, as shown in figure 5; when the driving rod 4 moves backwards to remove the pressure on the sealing film 3, the thimble valve opens, as shown in fig. 4.
In some embodiments of the present application, referring to fig. 2 and 3, the bottom of the dosing chamber 200 is funnel-shaped to facilitate the inflow and outflow of liquid.
In some embodiments of the present application, referring to fig. 6, the cartridge body 1 includes a cartridge plate 11 and a front sealing film 12. The surface of the cartridge board 11 is provided with a plurality of grooves, and the front sealing film 12 is attached to the surface of the cartridge board 11 to seal the grooves, so as to form a first chamber 241, a second chamber 242, a first connecting flow passage 221, a second connecting flow passage 222, a passage of the first valve 231, a passage of the second valve 232, the dosing cavity 200, the dosing cavity air passage 210, the first air passage 251, and the second air passage 252.
Referring to fig. 1, in the present embodiment, two sets of quantitative structure units are disposed on one cartridge body 1.
Referring to fig. 2, in the first set of quantitative structure units 2, the first chamber 241 is a sample solution chamber, and the second chamber 242 is a sample processing chamber. In this embodiment, the first chambers 241 have five chambers, which can be selectively connected to the quantitative chamber 200 by valve control, and the sample chamber can be pre-filled with lysis buffer, binding solution, cleaning solution and eluent. When the cracking step is carried out, quantitative lysate can be measured and put into the sample processing cavity; in performing the binding step, a measured amount of binding solution can be metered into the sample processing chamber. Set up quantitative between test solution chamber and sample processing chamber and get the structure, help controlling the test solution quantity of every step according to the sample volume, improve the detection accuracy.
Referring to fig. 3, in the second set of quantitative structure unit 2, the first chamber 241 is an eluent mixing chamber, and the second chamber 242 is a PCR chamber. In this embodiment, four PCR chambers are provided, and each PCR chamber can be selectively connected to the quantitative chamber 200 by valve control. In performing the amplification step, a measured amount of the eluent mixture can be metered into the PCR chamber. Set up the ration volume between eluent mixing chamber and PCR chamber and get the structure, help pushing the liquid volume in the PCR chamber according to the capacity control in PCR chamber to under the condition that is equipped with a plurality of PCR chambers, can be convenient for control to push the liquid volume in every PCR chamber the same, be convenient for contrast the amplification condition in every PCR chamber, be favorable to improving detection accuracy and sensitivity.
The above description is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and substitutions can be made without departing from the technical principle of the present invention, and these modifications and substitutions should also be regarded as the protection scope of the present invention.
Claims (10)
1. The card box with the quantitative measuring structure is characterized by comprising a card box body (1) and at least one group of quantitative structure units (2) arranged on the card box body (1), wherein each quantitative structure unit (2) comprises a first cavity (241), a second cavity (242), a first connecting flow channel (221), a second connecting flow channel (222), a first valve (231), a second valve (232), a quantitative cavity (200) and a quantitative cavity air channel (210);
the first chamber (241) is communicated with the bottom of the quantitative cavity (200) through the first connecting flow channel (221), and the first valve (231) is arranged on the first connecting flow channel (221);
the second chamber (242) is communicated with the bottom of the quantitative cavity (200) through the second connecting flow channel (222), and the second valve (232) is arranged on the second connecting flow channel (222);
one end of the quantitative cavity air channel (210) is provided with an air vent (211), and the other end of the quantitative cavity air channel is arranged at the top of the quantitative cavity (200).
2. The cartridge with a quantitative measuring structure according to claim 1, wherein a transition flow channel (212) is provided at a bottom center line of the quantitative cavity (200), and the first connection flow channel (221) and the second connection flow channel (222) are respectively communicated with the transition flow channel (212).
3. A cartridge having a quantitative dosing structure according to claim 1, wherein the first chamber (241) is provided with a first air channel (251), the first air channel (251) having a first air opening (261).
4. A cartridge having a quantitative dosing structure according to claim 3, wherein the first connection flow channel (221) communicates with the bottom of the first chamber (241), and the first air channel (251) is provided at the top of the first chamber (241).
5. A cartridge having a quantitative tapping structure according to claim 1, wherein the second chamber (241) is provided with a second air passage (252), the second air passage (252) having a second air port (262).
6. The cartridge of claim 5, wherein the second connection flow path (222) is communicated with a bottom of the second chamber (242), and the second air passage (252) is formed at a top of the second chamber (242).
7. The cartridge with a quantitative measuring structure according to claim 1, wherein the first valve (231) and the second valve (232) are needle-lift valves.
8. A cartridge having a quantitative measuring structure according to claim 7, wherein the first valve (231) has a passage therein, and a sealing film (3) is provided on the passage, and the sealing film (3) is deformed by a predetermined pressure to close the passage.
9. The cartridge with a quantitative dosing structure according to claim 1, wherein the bottom of the dosing chamber (200) is funnel-shaped.
10. The cartridge with a quantitative measuring structure according to claim 1, wherein the cartridge body (1) comprises a cartridge plate (11) and a front sealing film (12);
the surface of the card box plate (11) is provided with a plurality of grooves, the front sealing film (12) is attached to the surface of the card box plate (11) to seal the grooves, so that the first cavity (241), the second cavity (242), the first connecting flow channel (221), the second connecting flow channel (222), the quantitative cavity (200) and the quantitative cavity air channel (210) are formed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121112550.4U CN215799548U (en) | 2021-05-21 | 2021-05-21 | Card box with quantitative measuring structure |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121112550.4U CN215799548U (en) | 2021-05-21 | 2021-05-21 | Card box with quantitative measuring structure |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN215799548U true CN215799548U (en) | 2022-02-11 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202121112550.4U Active CN215799548U (en) | 2021-05-21 | 2021-05-21 | Card box with quantitative measuring structure |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN215799548U (en) |
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2021
- 2021-05-21 CN CN202121112550.4U patent/CN215799548U/en active Active
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