CN219117440U - Sample adding device for instant detection and nucleic acid detection system - Google Patents
Sample adding device for instant detection and nucleic acid detection system Download PDFInfo
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- CN219117440U CN219117440U CN202223596846.5U CN202223596846U CN219117440U CN 219117440 U CN219117440 U CN 219117440U CN 202223596846 U CN202223596846 U CN 202223596846U CN 219117440 U CN219117440 U CN 219117440U
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- tip
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- 238000001514 detection method Methods 0.000 title claims abstract description 59
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 29
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 29
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 29
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 31
- 238000007789 sealing Methods 0.000 claims abstract description 20
- 239000007787 solid Substances 0.000 claims abstract description 20
- 238000004891 communication Methods 0.000 claims abstract description 11
- 230000003321 amplification Effects 0.000 claims description 15
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 15
- 239000008188 pellet Substances 0.000 claims description 9
- 230000000717 retained effect Effects 0.000 claims description 2
- 239000000523 sample Substances 0.000 description 57
- 238000000034 method Methods 0.000 description 7
- 239000012488 sample solution Substances 0.000 description 7
- 238000010586 diagram Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000005070 sampling Methods 0.000 description 3
- 239000000443 aerosol Substances 0.000 description 2
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 2
- 229910052782 aluminium Inorganic materials 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 238000004090 dissolution Methods 0.000 description 2
- 239000011888 foil Substances 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 238000012123 point-of-care testing Methods 0.000 description 2
- 238000011897 real-time detection Methods 0.000 description 2
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000012631 diagnostic technique Methods 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000001821 nucleic acid purification Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000009736 wetting Methods 0.000 description 1
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a sample adding device for instant detection and a nucleic acid detection system, which comprises a pipetting module, wherein the pipetting module comprises a cylinder, a piston rod and a piston, the piston is arranged in the cylinder in a vertically movable manner, the piston is sealed with the inner wall of the cylinder, and the lower end part of the cylinder is provided with an air flow communication interface; the sample adding device further comprises a suction head module, and the suction head module comprises: a suction head; a solid-state detection reagent; a seal; the sample adding device is provided with an initial state and a working state, wherein in the initial state, the pipetting module and the suction head module are mutually separated, and the sealing piece seals the containing cavity; in the working state, the sealing element is broken or removed, and the air flow communication interface of the cylinder body is inserted into the upper end part of the suction head and is communicated with the containing cavity. The sample adding device for the instant detection can effectively avoid the pollution of the solid detection reagent.
Description
Technical Field
The utility model belongs to the technical field of nucleic acid detection, and particularly relates to a sample adding device for instant detection and a nucleic acid detection system.
Background
Nucleic acid detection, which is a molecular diagnostic technique that directly detects genetic material of an organism such as DNA, RNA, includes the main steps of sample cleavage, purification, and amplification, involves at least one pipetting process, for example, a process in which a liquid in a sample collection tube is transferred into a reaction vessel to be mixed with a nucleic acid amplification reagent or the like. It is highly necessary to avoid contamination of the nucleic acid amplification reagents prior to pipetting, otherwise the detection results may be affected.
Disclosure of Invention
In order to solve the above-mentioned problems, one of the objects of the present utility model is to provide a sample loading device for instant detection, which can avoid the contamination of the solid detection reagent.
Another object of the present utility model is to provide a nucleic acid detecting system that is simple to operate and convenient to use.
In order to achieve the above purpose, the utility model adopts the following technical scheme:
the sample adding device for the instant detection comprises a pipetting module, wherein the pipetting module comprises a cylinder, a piston rod capable of moving up and down relative to the cylinder and a piston connected to the piston rod, the piston is arranged in the cylinder in a manner of moving up and down, the piston is sealed with the inner wall of the cylinder, and the lower end part of the cylinder is provided with an air flow communication interface; the sample adding device further comprises a suction head module, and the suction head module comprises:
the suction head is provided with a containing cavity and a sample inlet communicated with the containing cavity, and the sample inlet is arranged at the lower end part of the suction head;
a solid state detection reagent disposed in the cavity;
a seal for sealing the cavity and capable of being broken or removed;
the sample adding device is provided with an initial state and a working state, wherein in the initial state, the pipetting module and the suction head module are mutually separated, and the sealing piece seals the containing cavity; in the working state, the sealing element is broken or removed, and the air flow communication interface of the cylinder body is inserted into the upper end part of the suction head and is communicated with the containing cavity.
Preferably, the suction head module further comprises a filter element, and the filter element is arranged in the upper end part of the suction head. The filter element can prevent aerosol from overflowing upwards.
Preferably, the filter element is located above the solid detection reagent and is disposed within the tip in a manner that prevents the solid detection reagent from falling out.
More preferably, the cartridge is retained within the upper end of the cleaner head.
Preferably, the solid detection reagent comprises a lyophilized pellet.
More preferably, the lyophilized pellet is a nucleic acid amplification reagent lyophilized pellet. The method can carry out re-dissolution and mixing on the freeze-dried balls of the nucleic acid amplification reagent while pipetting, reduces pipetting times in the whole detection flow, simplifies operation and avoids excessive introduction of operation errors.
Preferably, the lower end of the suction head is provided with a sample adding head which can be inserted into the detection device, and the outer diameter of the sample adding head is gradually reduced from top to bottom; the sample inlet is arranged in the sample adding head, and the inner diameter of the sample inlet is smaller than the inner diameter of the containing cavity. The diameter of the sample inlet is smaller, so that the sample solution can be conveniently sucked by inserting the sample inlet into the sampling tube.
Preferably, the seal is a sealed bag in which the tip is placed in the initial state.
More preferably, the material of the rice waiting is aluminum foil.
Preferably, the cylinder is provided with graduations.
The embodiment also provides a nucleic acid detection system, which comprises a microfluidic chip for nucleic acid amplification detection, and the nucleic acid detection system further comprises the sample adding device for instant detection, wherein the working state of the sample adding device comprises a first working state and a second working state, and the sample inlet and the microfluidic chip are mutually separated in the first working state; and in the second working state, the sample inlet is inserted into a sample inlet or a sample cavity of the microfluidic chip.
Compared with the prior art, the utility model has the following advantages:
according to the sample adding device for the instant detection, the solid detection reagent is arranged in the accommodating cavity, and the sealing piece seals the accommodating cavity in an initial state, so that the solid detection reagent is prevented from being polluted, even spoiled due to contact with air, moisture and the like, and long-term storage is realized; when in a working state, the sealing element is damaged or removed, the flow communication port is inserted into the upper end part of the suction head and is communicated with the containing cavity, the use is convenient, and meanwhile, the device is simple in structure and low in cost.
Drawings
In order to more clearly illustrate the technical solutions of the present utility model, the drawings that are needed in the description of the embodiments will be briefly described below, it being obvious that the drawings in the following description are only some embodiments of the present utility model, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic perspective view of a nucleic acid detecting system according to an embodiment of the present utility model;
FIG. 2 is a schematic diagram of a sample loading device for real-time detection according to an embodiment of the present utility model;
FIG. 3 is a schematic diagram of a pipetting module;
FIG. 4 is a schematic diagram of a tip module;
fig. 5 is a cross-sectional view taken along A-A in fig. 4.
Wherein,,
100. a microfluidic chip; 101. a sample adding port;
1. a pipetting module; 11. a cylinder; 111. an airflow communication interface; 12. a piston rod;
2. a suction head module; 21. a suction head; 211. a cavity; 212. a sample inlet; 213. a sample adding head; 22. a solid-state detection reagent; 23. a filter element.
Detailed Description
Preferred embodiments of the present utility model will be described in detail below with reference to the attached drawings so that the advantages and features of the present utility model can be more easily understood by those skilled in the art. The description of these embodiments is provided to assist understanding of the present utility model, but is not intended to limit the present utility model. In addition, technical features of the embodiments of the present utility model described below may be combined with each other as long as they do not collide with each other.
Referring to fig. 2 to 4, the present embodiment provides a sample loading device for real-time detection, which includes a pipetting module 1 and a pipette module 2.
Referring to fig. 3, further, the pipetting module 1 includes a cylinder 11, a piston rod 12 movable up and down with respect to the cylinder 11, and a piston (not shown) connected to the piston rod 12, the piston being provided in the cylinder 11 so as to be movable up and down, and being sealed between the piston and an inner wall of the cylinder 11.
Further, the lower end portion of the cylinder 11 is provided with an air flow communication port 111. The cylinder 11 is also provided with scales, so that quantitative liquid suction and quantitative sample solution injection can be realized according to actual application requirements.
Referring to fig. 4 to 5, the tip module 2 includes a tip 21, a plurality of solid detection reagents 22, a filter element 23, and a sealing member (not shown).
Further, the suction head 21 has a cavity 211 and a sample inlet 212 communicated with the cavity 211, the sample inlet 212 is arranged at the lower end part of the suction head 21, and a plurality of solid detection reagents 22 are placed in the cavity 211. The sealing piece is a sealing bag, and the sealing bag is preferably made of aluminum foil. The sealing bag is used to seal the cavity 211 and can be broken or removed.
The lower end of the suction head 21 is provided with a sample adding head 213 which can be inserted into the detection device, the outer diameter of the sample adding head 213 gradually decreases from top to bottom, a sample inlet 212 is formed in the sample adding head 213, and the inner diameter of the sample inlet 212 is smaller than the inner diameter of the cavity 211.
The filter element 23 is positioned above the solid detection reagent 22 and is clamped in the upper end part of the suction head 21, and the filter element 23 can prevent the solid detection reagent 22 from falling out on one hand and limit a plurality of solid detection reagents 22 in the accommodating cavity 211; on the other hand, after the sample solution is sucked, the secondary pollution caused by upward overflow of aerosol can be prevented.
The solid detection reagent 22 includes a lyophilized pellet, specifically, the lyophilized pellet of the present embodiment is a lyophilized pellet of a nucleic acid amplification reagent. The method can carry out re-dissolution and mixing on the freeze-dried balls of the nucleic acid amplification reagent while pipetting, reduces pipetting times in the whole detection flow, simplifies operation and avoids excessive introduction of operation errors.
The sample adding device has an initial state and a working state, in the initial state, the suction head 21 is placed in a sealed bag, the pipetting module 1 and the suction head module 2 are separated from each other, the sealing piece seals the containing cavity 211, the solid detection reagent is prevented from being polluted, even spoiled due to contact with air, moisture and the like, and long-term storage is realized without wetting; in the working state, the sealing element is broken or removed, the air flow communication interface 111 of the cylinder 11 is inserted into the upper end part of the suction head 21 and is communicated with the containing cavity 211, the operation is simple, the use is convenient, and the detection efficiency is high.
Referring to fig. 1, the present embodiment further provides a nucleic acid detection system, including a microfluidic chip 100 for detecting nucleic acid amplification, where the nucleic acid detection system further includes the sample adding device for immediate detection, and the working state of the sample adding device includes a first working state and a second working state, and in the first working state, the sample inlet 212 and the microfluidic chip 100 are separated from each other; in the second working state, the sample inlet 212 is inserted into the sample inlet 101 or the sample cavity of the microfluidic chip 100, and the inner diameter of the sample inlet 212 is smaller than the inner diameter of the containing cavity 211, so that not only is the liquid conveniently sucked in the sampling tube, but also the sample can be better inserted into the sample inlet 101. In addition, the inner diameter of the sample inlet can be adjusted according to the size of the sample inlet of the microfluidic chip.
The working process of the sample adding device for instant detection in this embodiment is as follows: firstly, the sealing element is broken or removed, the air flow communication interface 111 of the suction head 21 is inserted into the upper end part of the suction head 11 and is communicated with the containing cavity 211, then the piston rod 12 is pulled upwards to move upwards relative to the suction head 11, a certain amount of nucleic acid sample solution (such as preserving fluid such as a throat swab) is sucked into a sampling tube by the sample inlet 212 of the suction head 21, the sample solution and a plurality of nucleic acid amplification reagents in the containing cavity 211 are freeze-dried and mixed, finally, the sample inlet 212 of the suction head 21 is inserted into the sample inlet 101 or the sample cavity of the microfluidic chip 100, the piston rod 12 is pushed downwards to move downwards relative to the suction head 11, and the mixed sample solution is injected. The device has simple integral structure, lower cost and more convenient use.
The reaction components required by nucleic acid amplification are pre-buried in the accommodating cavity 211, so that after the sample diluent is sucked once, the sample can be directly mixed and lyophilized to be added into a microfluidic chip or a POCT (point of care testing) device, and the method is particularly suitable for the use scene without nucleic acid purification and sample direct amplification, and is very necessary for mass qualitative screening of nucleic acid.
In the initial state of the sample adding device of the embodiment, the sealing member seals the accommodating cavity 211, so that the solid detection reagent 22 is prevented from being polluted due to contact with air, moisture and the like; the cylinder 11 of the pipetting module 1 is provided with scales, so that the sample solution can be quantitatively sucked and quantitatively injected; the plurality of nucleic acid amplification reagent freeze-dried balls are arranged in the containing cavity 211, and can be re-dissolved and mixed while pipetting, so that pipetting times in the whole detection flow are reduced, operation is simplified, excessive introduction of operation errors is avoided, operation is simple, detection efficiency is high, flexibility is high, and especially an application scene of instant portable detection is realized.
As used in this specification and in the claims, the terms "comprises" and "comprising" merely indicate that the steps and elements are explicitly identified, and do not constitute an exclusive list, as other steps or elements may be included in a method or apparatus. The term "and/or" as used herein includes any combination of one or more of the associated listed items.
It should be noted that, unless otherwise specified, when a feature is referred to as being "fixed" or "connected" to another feature, it may be directly or indirectly fixed or connected to the other feature. Further, the descriptions of the upper, lower, left, right, etc. used in the present utility model are merely with respect to the mutual positional relationship of the constituent elements of the present utility model in the drawings.
The above-described embodiments are provided for illustrating the technical concept and features of the present utility model, and are intended to be preferred embodiments for those skilled in the art to understand the present utility model and implement the same according to the present utility model, not to limit the scope of the present utility model. All equivalent changes or modifications made according to the principles of the present utility model should be construed to be included within the scope of the present utility model.
Claims (10)
1. The sample adding device for the instant detection comprises a pipetting module, wherein the pipetting module comprises a cylinder, a piston rod capable of moving up and down relative to the cylinder and a piston connected to the piston rod, the piston is arranged in the cylinder in a manner of moving up and down, the piston is sealed with the inner wall of the cylinder, and the lower end part of the cylinder is provided with an air flow communication interface; the sample adding device is characterized by further comprising a suction head module, wherein the suction head module comprises:
the suction head is provided with a containing cavity and a sample inlet communicated with the containing cavity, and the sample inlet is arranged at the lower end part of the suction head;
a solid state detection reagent disposed in the cavity;
a seal for sealing the cavity and capable of being broken or removed;
the sample adding device is provided with an initial state and a working state, wherein in the initial state, the pipetting module and the suction head module are mutually separated, and the sealing piece seals the containing cavity; in the working state, the sealing element is broken or removed, and the air flow communication interface of the cylinder body is inserted into the upper end part of the suction head and is communicated with the containing cavity.
2. The sample addition device of claim 1, wherein the tip module further comprises a filter element disposed within an upper end of the tip.
3. The sample application device according to claim 2, wherein the filter element is located above the solid detection reagent and is disposed in the tip so as to prevent the solid detection reagent from falling out.
4. A sample application device according to claim 3, wherein the cartridge is retained within an upper end of the tip.
5. The sample addition device of claim 1, wherein the solid detection reagent comprises a lyophilized pellet.
6. The sample addition device of claim 5, wherein the lyophilized pellet is a lyophilized pellet of nucleic acid amplification reagents.
7. The sample application device according to claim 1, wherein the tip has a tip at a lower end portion thereof, the tip having an outer diameter which is gradually reduced from top to bottom, the tip being insertable into the detection device; the sample inlet is arranged in the sample adding head, and the inner diameter of the sample inlet is smaller than the inner diameter of the containing cavity.
8. The sample application device according to claim 7, wherein the sealing member is a sealed bag, and the tip is placed in the sealed bag in an initial state.
9. The sample application device according to claim 1, wherein the cylinder is provided with a scale.
10. A nucleic acid detection system comprising a microfluidic chip for nucleic acid amplification detection, wherein the nucleic acid detection system further comprises a sample addition device for immediate detection according to any one of claims 1 to 9, the operating state of the sample addition device comprising a first operating state in which the sample inlet and the microfluidic chip are separated from each other and a second operating state; and in the second working state, the sample inlet is inserted into a sample inlet or a sample cavity of the microfluidic chip.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223596846.5U CN219117440U (en) | 2022-12-30 | 2022-12-30 | Sample adding device for instant detection and nucleic acid detection system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223596846.5U CN219117440U (en) | 2022-12-30 | 2022-12-30 | Sample adding device for instant detection and nucleic acid detection system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219117440U true CN219117440U (en) | 2023-06-02 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202223596846.5U Active CN219117440U (en) | 2022-12-30 | 2022-12-30 | Sample adding device for instant detection and nucleic acid detection system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219117440U (en) |
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- 2022-12-30 CN CN202223596846.5U patent/CN219117440U/en active Active
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