CN111607483A - In-vitro diagnostic instrument and extraction device - Google Patents
In-vitro diagnostic instrument and extraction device Download PDFInfo
- Publication number
- CN111607483A CN111607483A CN202010378138.0A CN202010378138A CN111607483A CN 111607483 A CN111607483 A CN 111607483A CN 202010378138 A CN202010378138 A CN 202010378138A CN 111607483 A CN111607483 A CN 111607483A
- Authority
- CN
- China
- Prior art keywords
- liquid supply
- outlet
- extraction
- unit
- inlet
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Abstract
The invention discloses an in vitro diagnostic apparatus and an extraction device, wherein the extraction device comprises: the device comprises an extracting part, a liquid supply unit, a control valve unit, an adsorption unit and a power unit, wherein the extracting part is provided with an extracting cavity and an inlet end and an outlet end communicated with the extracting cavity, the liquid supply unit is communicated with the inlet end and the outlet end, the control valve unit is arranged between the liquid supply unit and the inlet end and the outlet end and used for controlling the liquid supply unit to be communicated and disconnected with the inlet end and the outlet end, the adsorption unit is arranged between an outlet of the control valve and the extracting cavity, and the power unit is used for providing negative pressure and positive pressure. The extraction device is simple and convenient to operate, and can improve the binding rate of biomolecules. This external diagnostic apparatus has adopted this extraction element, can carry out the preliminary screening and detect.
Description
Technical Field
The invention relates to the technical field of in-vitro diagnosis, in particular to an in-vitro diagnostic apparatus and an extraction device.
Background
At present, the extraction of biomolecules from biological samples for specific detection needs to be performed in a laboratory by a complicated analysis instrument and can be completed by professionals through a plurality of operation steps, which results in long time consumption for the analysis of the detection result. For example, in nucleic acid extraction, four steps of lysis, binding, washing, elution, and the like are generally required for specific nucleic acid detection, and the subsequent detection steps of nucleic acid molecular hybridization, Polymerase Chain Reaction (PCR), biochip, and the like are added, so that the whole detection process is time-consuming and long.
When the specific detection needs to be developed in a large scale in a short time, how to simplify the operation steps and quickly obtain the extract capable of being primarily screened is of great significance. The conventional extraction apparatus for this block can simplify the operation steps, but the binding rate of biomolecules is low.
Disclosure of Invention
In view of the above, it is necessary to provide an in vitro diagnostic apparatus and an extraction device. The extraction device is simple and convenient to operate, and can improve the binding rate of biomolecules. This external diagnostic apparatus has adopted this extraction element, can carry out the preliminary screening and detect.
The technical scheme is as follows:
in one aspect, the present application provides an extraction device comprising:
the extraction piece is provided with an extraction cavity and an inlet end and an outlet end which are communicated with the extraction cavity;
the liquid supply unit is communicated with the inlet end and the outlet end;
the control valve unit is arranged between the liquid supply unit and the inlet and outlet ends and is used for controlling the liquid supply unit to be communicated and disconnected with the inlet and outlet ends;
the adsorption unit is arranged between the outlet of the control valve unit and the extraction cavity; and
and the power unit is used for providing negative pressure and positive pressure for the extraction cavity.
When the extraction device is used, the sample liquid is stored in the liquid supply unit, the preset reagent liquid and the sample liquid are added for mixing and decomposing to obtain biomolecules, and in the process, the liquid supply unit and the inlet and outlet ends are in a disconnected state. After the decomposition of completion biomolecule, utilize the control valve unit to make and supply liquid unit and business turn over end intercommunication, then the power pack provides the negative pressure for drawing the chamber for above-mentioned mixed liquid can be toward the flow direction and draw the chamber, and this in-process can be through the adsorption unit, and the biomolecule after the decomposition can be adsorbed by the adsorption unit. Above-mentioned mixed liquid is whole through or most through adsorbing the unit entering and draw the chamber after, can let the power unit provide the malleation for drawing the chamber for mixed liquid is discharged from drawing the chamber, and this process again will be through adsorbing the unit, utilizes the unit that adsorbs further to adsorb biomolecule, and then can improve biomolecule's binding rate. Of course, the above operations may be cycled again as needed. And in the process of pushing the mixed liquid out of the extraction cavity for the last time, the control valve unit is used for controlling the disconnection of the inlet and outlet end and the liquid supply unit, and other outlets are opened, so that the mixed liquid is discharged. The extraction device is simple and convenient to operate, and can improve the binding rate of biomolecules.
Further, in combination with the requirement of the next extraction step, other reagents can be injected into the liquid supply unit to perform the next treatment on the biomolecules in the adsorption unit.
The technical solution is further explained below:
in one embodiment, the control valve unit includes a first check valve openable only toward the extraction chamber, and a first on-off valve for controlling communication and disconnection of the liquid supply unit with the inlet and outlet, the first check valve being disposed near the inlet and outlet with respect to the first on-off valve, and the adsorption unit being disposed between an outlet of the first check valve and the extraction chamber.
In one embodiment, the liquid supply unit comprises a first liquid supply part and a second liquid supply part, an outlet of the first liquid supply part and an outlet of the second liquid supply part are respectively communicated with the liquid supply unit, and first switch valves are respectively arranged between the outlets of the first liquid supply part and the second liquid supply part and the liquid supply unit;
or the liquid supply unit comprises a third liquid supply part, the outlet of the third liquid supply part is communicated with the inlet end and the outlet end, the first switch valve is arranged between the outlet of the third liquid supply part and the inlet end and the outlet end, and the third liquid supply part is provided with a detachable cover body.
In one embodiment, the first liquid supply member is used for storing lysis solution, and the second liquid supply member is used for storing eluent.
In one embodiment, the liquid supply unit further comprises a fourth liquid supply part for storing the cleaning liquid, an outlet of the fourth liquid supply part is communicated with the inlet end and the outlet end, and a first switch valve is arranged between the outlet of the fourth liquid supply part and the inlet end and between the outlet of the fourth liquid supply.
In one embodiment, the first liquid supply is provided with a second one-way valve openable only towards the interior of the first liquid supply, or/and the second liquid supply is provided with a third one-way valve openable only towards the interior of the second liquid supply.
In one embodiment, the extraction device further comprises a cavity, the cavity is communicated with the inlet end and the outlet end, and a second switch valve for on-off control is arranged between the cavity and the inlet end and the outlet end.
In one embodiment, the adsorption unit is an adsorption film or a magnetic bead.
In one embodiment, the power unit is a piston pump, and an inlet and an outlet of the piston pump are communicated with the extraction cavity; or the power unit and the extraction part form an injector structure, and the power unit is provided with a piston rod matched with the extraction cavity; or the power unit is a dropper, and an inlet and an outlet of the dropper are communicated with the extraction cavity; or the power unit and the extraction part form a dropper structure, and the power unit is provided with an elastic rubber cap matched with the extraction cavity.
In one embodiment, the extraction device further comprises an auto-heating unit for heating the liquid supply unit, the auto-heating unit being disposed outside the liquid supply unit.
On the other hand, the application also provides an in-vitro diagnostic apparatus which comprises the extraction device in any embodiment.
The in vitro diagnostic apparatus adopts the extraction device, and can obtain biomolecules by using the extraction device to carry out primary screening detection. In addition, the extraction device is simple and convenient to operate, can be used in various occasions (such as families and communities), enables the in vitro diagnostic instrument to be more convenient to use, and can be used for primary screening of specificity detection without professional on duty training.
The technical solution is further explained below:
in one embodiment, the in-vitro diagnostic apparatus further comprises a detection test paper, a communication structure is arranged between the sample dropping area of the detection test paper and the extraction cavity, and the communication structure is provided with a third switch valve for on-off control.
Drawings
FIG. 1 is a schematic structural diagram of an extracting apparatus according to a first embodiment;
FIG. 2 is a schematic sectional view of the extraction apparatus according to the second embodiment in an inoperative state;
FIG. 3 is a schematic view of the extraction device of FIG. 2 with a first liquid supply open;
FIG. 4 is a schematic view of the extraction apparatus of FIG. 2 with a first liquid supply being pumped;
FIG. 5 is a schematic view of a second liquid supply of the extraction device of FIG. 2 in an open state;
FIG. 6 is a schematic view of a second liquid supply being pumped by the extraction device shown in FIG. 2;
FIG. 7 is a schematic structural view of an extracting apparatus according to a third embodiment;
FIG. 8 is a schematic structural view of an extracting apparatus according to a fourth embodiment;
fig. 9 is a schematic structural view of an extraction device according to the fifth embodiment.
Description of reference numerals:
100. extracting the part; 110. an extraction chamber; 120. an inlet and an outlet; 200. a liquid supply unit; 210. a first liquid supply member; 212. a second one-way valve; 220. a second liquid supply member; 222. a third check valve; 230. a third liquid supply member; 232. a cover body; 240. a fourth liquid supply member; 300. a control valve unit; 310. a first check valve; 320. a first on-off valve; 400. an adsorption unit; 500. a power unit; 510. a piston rod; 600. a cavity; 610. a second on-off valve; 700. a mounting unit; 800. a waste liquid cavity; 810. and a fourth check valve.
Brief description of the drawingsthe accompanying drawings, which form a part of this application, are included to provide a further understanding of the invention, and are included to explain illustrative embodiments of the invention and the description thereof and are not to be considered limiting of the invention.
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without creative efforts.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail below with reference to the accompanying drawings and the detailed description. It should be understood that the detailed description and specific examples, while indicating the scope of the invention, are intended for purposes of illustration only and are not intended to limit the scope of the invention.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.
As shown in fig. 1 and fig. 2, in an embodiment, an extraction apparatus is provided, which includes: the extraction tool 100, the liquid supply unit 200, the control valve unit 300, the adsorption unit 400, and the power unit 500. The extraction member 100 is provided with an extraction chamber 110, and an access end 120 in communication with the extraction chamber 110. The liquid supply unit 200 communicates with the access port 120. The control valve unit 300 is disposed between the liquid supply unit 200 and the inlet/outlet end 120, and is used for controlling the liquid supply unit 200 to be connected to and disconnected from the inlet/outlet end 120. The adsorption unit 400 is disposed between the outlet of the control valve unit 300 and the extraction chamber 110. The power unit 500 is used to provide negative and positive pressure to the extraction chamber 110.
As shown in fig. 2 to 6, when the above extraction device is used, the sample solution is stored in the solution supply unit 200, and a predetermined reagent solution is added to mix with the sample solution to decompose biomolecules, during which the solution supply unit 200 is disconnected from the inlet/outlet end 120. After the decomposition of the biomolecules is completed, the control valve unit 300 is used to communicate the liquid supply unit 200 with the inlet and outlet 120, and then the power unit 500 provides negative pressure for the extraction cavity 110, so that the mixed liquid flows toward the extraction cavity 110, and passes through the adsorption unit 400 in the process, and the decomposed biomolecules are adsorbed by the adsorption unit 400. After all or most of the mixed liquid enters the extraction cavity 110 through the adsorption unit 400, the power unit 500 can provide positive pressure for the extraction cavity 110, so that the mixed liquid is discharged from the extraction cavity 110, the mixed liquid passes through the adsorption unit 400 again in the process, the adsorption unit 400 is used for further adsorbing biomolecules, and the binding rate of the biomolecules can be improved. Of course, the above operations may be cycled again as needed. During the last time of pushing the mixed liquid out of the extraction cavity 110, the control valve unit 300 is used to control the in-out end 120 to be disconnected from the liquid supply unit 200, and the other cavities are opened (as shown in fig. 2 and 9), so that the mixed liquid is discharged. The extraction device is simple and convenient to operate, and can improve the binding rate of biomolecules.
Further, in combination with the requirement of the next extraction step, other reagents may be injected into the liquid supply unit 200 to perform the next treatment on the biomolecules in the adsorption unit 400, which may refer to the same or equivalent embodiments shown in fig. 1, fig. 2, fig. 7, fig. 8 and fig. 9.
The sample fluid can be obtained directly or indirectly from a nasopharyngeal swab, a sputum, urine, feces, cervical/vaginal swab, blood, cerebrospinal fluid, skin, wound swab, or the like.
It should be noted that the "liquid supply unit 200" includes, but is not limited to, liquid supply tubes, liquid supply bags, liquid supply boxes, etc. capable of storing or injecting liquid, and the number of the liquid supply tubes, the liquid supply bags, the liquid supply boxes, etc. may be set according to needs, such as 1, 2, 3, 4, etc. Specifically, reference may be made to fig. 1, fig. 2, fig. 7 and fig. 8.
The "control valve unit 300" is one of the prior arts that can realize on-off control or flow direction control of liquid, and may be a single type of on-off valve, or a combination of a check valve and an on-off valve, as long as the above requirements can be met.
The "adsorption unit 400" may be any conventional adsorption functional member capable of adsorbing biomolecules.
Specifically, in this embodiment, the adsorption unit 400 is an adsorption film or/and a magnetic bead.
It should be noted that the "power unit 500" is any existing device capable of providing suction gas to the extraction chamber 110 to form negative pressure or injecting gas to form positive pressure.
Alternatively, in an embodiment, the power unit 500 is a piston pump, and an inlet and an outlet of the piston pump are communicated with the extraction chamber 110; or the power unit 500 and the extraction member 100 form a syringe structure, the power unit 500 is provided with a piston rod 510 matched with the extraction cavity 110; or the power unit 500 is a dropper, and the inlet and the outlet of the dropper are communicated with the extraction cavity 110; or the power unit 500 and the extraction member 100 form a dropper structure, and the power unit 500 is provided with an elastic rubber cap matched with the extraction cavity 110.
In the present embodiment, the power unit 500 and the extraction member 100 form a syringe structure, and the power unit 500 is provided with a piston rod 510 engaged with the extraction chamber 110.
For a better illustration of the extraction apparatus of the present application, reference is made below to nucleic acid extraction.
As shown in fig. 2 to 4, in combination with the foregoing description, the liquid supply unit 200 of the present embodiment has a liquid supply function. When the extraction device is used for extracting nucleic acid, the sample liquid is stored in the liquid supply unit 200, the lysis solution is added to be mixed with the sample liquid, nucleic acid molecules are lysed, and in the process, the liquid supply unit 200 and the inlet and outlet end 120 are in a disconnected state. After the cracking of the nucleic acid molecules is completed, the control valve unit 300 is used to communicate the liquid supply unit 200 with the inlet and outlet 120, and then the power unit 500 provides negative pressure for the extraction cavity 110, so that the mixed liquid containing the nucleic acid molecules flows to the extraction cavity 110, and in the process, the nucleic acid molecules pass through the adsorption unit 400, and are adsorbed by the adsorption unit 400. After all or most of the mixed liquid enters the extraction cavity 110 through the adsorption unit 400, the power unit 500 can provide positive pressure for the extraction cavity 110, so that the mixed liquid is discharged from the extraction cavity 110 (can enter the liquid supply unit 200 or other preset cavities), and in the process, the mixed liquid passes through the adsorption unit 400 again, and the adsorption unit 400 is used for further adsorbing nucleic acid molecules, so that the binding rate of the nucleic acid molecules can be improved. Of course, the above operations may be cycled again as needed. During the last time of pushing out the mixed liquid from the extraction cavity 110, the control valve unit 300 is used to control the in-out end 120 to be disconnected from the liquid supply unit 200, and other outlets are opened, so that the mixed liquid is discharged.
As shown in fig. 5 and fig. 6, in combination with the requirement of the next extraction step, an eluent may be injected into the liquid supply unit 200 to elute the adsorption unit 400, and the process may be repeated to perform the suction and push operations, so that the nucleic acid molecules adsorbed on the adsorption unit 400 are sufficiently eluted to obtain the desired extract.
Of course, the washing solution may be injected first, and after the washing is completed, the eluent may be injected to obtain the extracting solution.
Of course, in other embodiments, the liquid supply unit 200 may pre-store the required extraction reagent solution, and inject the sample solution into the lysis solution.
In addition to any of the above embodiments, as shown in fig. 1 and 2, in one embodiment, the control valve unit 300 includes a first check valve 310 that is only openable toward the extraction chamber 110, and a first on-off valve 320 for controlling the liquid supply unit 200 to be connected to and disconnected from the inlet/outlet 120, the first check valve 310 is disposed near the inlet/outlet 120 with respect to the first on-off valve 320, and the adsorption unit 400 is disposed between an outlet of the first check valve 310 and the extraction chamber 110. In this way, by using the first check valve 310, the liquid can only flow towards the extraction cavity 110, and the liquid pushed out from the extraction cavity 110 can not return to the original liquid supply unit 200, so that the operation steps can be further optimized, and the operation is convenient for the ordinary people. And only the opening or closing operation is needed by the first switch valve 320, so that the extraction process is easy to understand and control.
Based on the above-mentioned embodiment of the control valve unit 300, as shown in fig. 1 and fig. 2, in an embodiment, the liquid supply unit 200 includes a first liquid supply member 210 and a second liquid supply member 220, an outlet of the first liquid supply member 210 and an outlet of the second liquid supply member 220 are respectively communicated with the inlet and outlet 120, and a first switch valve 320 is respectively disposed between the outlet of the first liquid supply member 210 and the outlet of the second liquid supply member 220 and the inlet and outlet 120. Furthermore, the first liquid supply part 210 and the second liquid supply part 220 can be controlled by the first switch valve 320, so that the extracting reagent can be pre-stored in the first liquid supply part 210 and the second liquid supply part 220.
As shown in fig. 2 to 6, optionally, in an embodiment, the first liquid supply 210 is used for storing lysis solution, and the second liquid supply 220 is used for storing eluent. Further, the nucleic acid extraction operation can be performed, the sample liquid can be injected into the first liquid supply member 210, after the lysis is completed, the first switch valve 320 is opened, the mixed liquid is injected into the extraction cavity 110, and in the process that the mixed liquid is pushed out of the extraction cavity 110, the liquid can not return to the first liquid supply member 210 by using the first one-way valve 310, so that the liquid can be prevented from entering the second liquid supply member 220 due to misoperation, and the operation steps can be set in sequence. After the combination step is completed, the eluent can be injected into the extraction cavity 110 by only opening the first switch valve 320 corresponding to the second liquid supply member 220, and the elution and extraction of the nucleic acid molecules on the adsorption unit 400 are completed in the process.
Further, as shown in fig. 8, in an embodiment, the liquid supply unit 200 further includes a fourth liquid supply part 240 for storing the cleaning liquid, an outlet of the fourth liquid supply part 240 is communicated with the inlet and outlet end 120, and a first switch valve 320 is disposed between the outlet of the fourth liquid supply part 240 and the liquid supply unit 200. Thus, specifically, in combination with the related operation steps of the second embodiment, the fourth liquid supply part 320 may be used to inject the cleaning solution in the fourth liquid supply part to complete the cleaning step before the elution, and then the elution step is performed, so that the extraction accuracy may be further improved.
On the basis of any one of the above embodiments of the liquid supply unit 200, as shown in fig. 1 and 2, in one embodiment, the first liquid supply part 210 is provided with the second one-way valve 212 which can only be opened towards the inside of the first liquid supply part 210, or/and the second liquid supply part 220 is provided with the third one-way valve 222 which can only be opened towards the inside of the second liquid supply part 220. Therefore, the one-way valve is utilized to ensure that the interior of the liquid supply part can be the same as the outside, so that the corresponding liquid can be conveniently pumped away.
In another embodiment, the liquid supply unit 200 includes a third liquid supply member 230, an outlet of the third liquid supply member 230 is connected to the inlet/outlet 120, a first switch valve 320 is disposed between the outlet of the third liquid supply member 230 and the inlet/outlet 120, and the third liquid supply member 230 is provided with a detachable cover 232. In this way, a corresponding extraction action can be performed by opening the cover 232 to add a desired reagent or sample to the third liquid supply member 230. Specifically, in the nucleic acid extraction, the third liquid supply member 230 may be used to sequentially add the required reagents, and the control valve unit 300 may be used to perform the aforementioned extraction operation.
The liquid supply member in the same embodiment may also be provided with a cover structure to facilitate the addition of liquid.
The specific structures of the first liquid supply member 210, the second liquid supply member 220, the third liquid supply member 230, and the fourth liquid supply member 240 may be liquid storage members such as a liquid supply tube, a liquid supply bag, and a liquid supply box.
On the basis of any of the above embodiments, as shown in fig. 9, in an embodiment, the extraction device further includes a cavity 600, the cavity 600 is communicated with the inlet and outlet end 120, and a second switch valve for on-off control is disposed between the cavity 600 and the inlet and outlet end 120. Thus, the cavity 600 can be used as a buffer cavity, so that liquid in the combining process can enter the waste liquid cavity for buffering. Specifically, when the combining step is required, the extracted liquid does not return to the liquid supply member due to the action of the check valve 310, and at this time, the second switch valve may be opened, so that the cavity 600 is communicated with the inlet and outlet 120, and the liquid pushed out from the extraction cavity 110 may enter the cavity 600 for buffering, and then be extracted for combining. After the bonding is completed, the waste liquid is pushed into the cavity 600; the second on-off valve is then closed. If the cleaning is needed, after the cleaning is completed, the second switch valve can be opened, and the used cleaning solution is pushed into the cavity 600 for storage.
In this case, the chamber 600 may be used as a waste liquid chamber and a buffer chamber, which can improve the coupling efficiency and store waste liquid.
Further, the chamber 600 may have a plurality of steps, i.e., the binding step, the washing step, and the elution step correspond to one chamber 600, so that the above steps may be repeated for suction. In particular, when elution is performed, the chamber 600 is repeatedly used for suction in this manner, thereby achieving a centrifugal effect.
As shown in fig. 2 to fig. 6, in the second embodiment, the extraction device further includes a waste liquid cavity 800, and a fourth check valve 810 is disposed between the waste liquid cavity 800 and the inlet/outlet end 120. So, can only need outer waste liquid cavity 800 to carry out the waste liquid storage, and easy operation can adapt to and draw the relatively lower molecule of requirement and draw.
In addition to any of the above embodiments, in one embodiment, the extracting apparatus further includes an auto-heating unit (not shown) for heating the liquid supply unit 200, the auto-heating unit being disposed outside the liquid supply unit 200. Therefore, the self-heating unit is used for providing heat for the sample liquid cracking process, the cracking speed and the cracking rate are improved, and the extraction efficiency and the extraction quality are improved.
The self-heating unit can be realized by adopting any self-heating bag meeting the requirements in the prior art, namely, the self-heating bag can be stored at the preset position of the liquid supply unit 200 and is excited by a preset means to react to generate heat.
On the basis of any of the above embodiments, as shown in fig. 1 and fig. 2, in an embodiment, the extraction device further includes a mounting unit 700, and further, the above structure can be integrally mounted on the mounting unit 700, so as to facilitate modular assembly. The specific structure of the mounting unit 700 may be various.
In the present embodiment, the mounting unit 700 is a closed box. Therefore, pollution can be avoided, and authenticity of detection data can be guaranteed.
In one embodiment, an in vitro diagnostic apparatus is further provided, which comprises the extraction device in any one of the above embodiments.
The in vitro diagnostic apparatus adopts the extraction device, and can obtain biomolecules by using the extraction device to carry out primary screening detection. In addition, the extraction device is simple and convenient to operate, can be used in various occasions (such as families and communities), enables the in vitro diagnostic instrument to be more convenient to use, and can be used for primary screening of specificity detection without professional on duty training.
On the basis of any of the above embodiments, in an embodiment, the in-vitro diagnostic apparatus further includes a detection test paper (not shown), a communication structure is arranged between the sample dropping area of the detection test paper and the extraction cavity 110, and the communication structure is provided with a third on-off valve for on-off control. Therefore, the extracting solution obtained in the extracting cavity 110 can be directly input into the detection test paper for detection by utilizing the connecting structure, and a primary screening result can be quickly obtained.
It should be noted that "unit" in the present application is an abbreviation of a combination having a specific function.
EXAMPLE 1 lysis solution extraction
The device of the invention respectively uses alkaline lysis solution and guanidinium lysis solution to extract enriched nucleic acid, which comprises the following steps:
(1) 2ul of plasmid is added into 1ml of lysis solution;
(2) adding the lysis solution containing the plasmid into the first liquid supply part 210;
(3) opening the first switch to communicate the first liquid supply member 210 with the inlet and outlet, and sucking the liquid in the first liquid supply member 210 into the extraction chamber 110 through the power unit 500, wherein the liquid passes through the adsorption unit 400 and the nucleic acid molecules are adsorbed by the adsorption unit 400; then pushing out the liquid, and in the process, adsorbing the nucleic acid molecules by using the adsorption unit 400 again;
(4) after the adsorption is completed, 100ul of eluent is added into the second liquid supply member 210;
(5) the eluent is sent to the extraction cavity through the power unit 500, and in the process, the nucleic acid molecules adsorbed by the adsorption unit 400 are eluted by the eluent, and the liquid containing the nucleic acid molecules is collected.
The results show that the concentration after enrichment is low under alkaline lysis solution conditions, and the binding efficiency of nucleic acid to silicon membrane is low under alkaline conditions. Under the condition of guanidine salt lysate, the nucleic acid and the silicon membrane have higher binding efficiency.
The device is used for enriching nucleic acid for 1 time without elution by using guanidinium lysate and the device of the invention; enriching for 1 time, and eluting once; enriching for 1 time, and eluting for 2 times; enrichment is carried out for 2 times without elution; enriching for 2 times, and eluting for 1 time; enrichment was performed 2 times and elution was performed 2 times. The concentration of each extracted nucleic acid was measured.
The results show that the concentration of nucleic acid is significantly increased in the two-time enrichment compared to the one-time enrichment. Therefore, the device of the invention is simple and convenient to operate, and the binding rate of the biomolecules is improved.
In the description of the present invention, it is to be understood that the terms "central," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," "circumferential," and the like are used in the orientations and positional relationships indicated in the drawings for convenience in describing the invention and to simplify the description, and are not intended to indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and are not to be considered limiting of the invention.
Furthermore, the terms "first", "second", "third", "fourth" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first," "second," "third," or "fourth" may explicitly or implicitly include at least one of the feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.
In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can, for example, be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
In the present invention, unless otherwise expressly stated or limited, the first feature "on" or "under" the second feature may be directly contacting the first and second features or indirectly contacting the first and second features through an intermediate. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature.
It will be understood that when an element is referred to as being "secured to," "disposed on," "secured to," or "disposed on" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. Further, when one element is considered as "fixed transmission connection" with another element, the two elements may be fixed in a detachable connection manner or in an undetachable connection manner, and power transmission can be achieved, such as sleeving, clamping, integrally-formed fixing, welding and the like, which can be achieved in the prior art, and is not cumbersome. When an element is perpendicular or nearly perpendicular to another element, it is desirable that the two elements are perpendicular, but some vertical error may exist due to manufacturing and assembly effects. The terms "vertical," "horizontal," "left," "right," and the like as used herein are for illustrative purposes only and do not represent the only embodiments.
The technical features of the above embodiments can be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the above embodiments are not described, but should be considered as the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
The above examples only show some embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (10)
1. An extraction device, comprising:
the extraction part is provided with an extraction cavity and an inlet end and an outlet end which are communicated with the extraction cavity;
the liquid supply unit is communicated with the inlet end and the outlet end;
the control valve unit is arranged between the liquid supply unit and the inlet and outlet ends and is used for controlling the liquid supply unit to be communicated and disconnected with the inlet and outlet ends;
an adsorption unit disposed between an outlet of the control valve unit and the extraction chamber; and
and the power unit is used for providing negative pressure and positive pressure for the extraction cavity.
2. The extraction apparatus as claimed in claim 1, wherein the control valve unit includes a first check valve openable only toward the extraction chamber, and a first on-off valve for controlling the liquid supply unit to be connected to and disconnected from the inlet and outlet ports, the first check valve being disposed near the inlet and outlet ports with respect to the first on-off valve, the adsorption unit being disposed between an outlet of the first check valve and the extraction chamber.
3. The extraction device according to claim 2, wherein the liquid supply unit comprises a first liquid supply member and a second liquid supply member, an outlet of the first liquid supply member and an outlet of the second liquid supply member are respectively communicated with the inlet and outlet ends, and the first switch valves are respectively arranged between the outlet of the first liquid supply member and the inlet and outlet ends;
or the liquid supply unit comprises a third liquid supply part, an outlet of the third liquid supply part is communicated with the inlet end and the outlet end, the first switch valve is arranged between the outlet of the third liquid supply part and the inlet end and the outlet end, and the third liquid supply part is provided with a detachable cover body.
4. The extraction apparatus as claimed in claim 3, wherein the first liquid supply member is configured to store a lysis solution and the second liquid supply member is configured to store an elution solution.
5. The extraction apparatus as claimed in claim 4, wherein the liquid supply unit further comprises a fourth liquid supply member for storing a cleaning liquid, an outlet of the fourth liquid supply member is communicated with the inlet and outlet ends, and the first switch valve is disposed between the outlet of the fourth liquid supply member and the inlet and outlet ends.
6. Extraction device according to claim 3, characterized in that the first liquid supply is provided with a second one-way valve which is openable only towards the interior of the first liquid supply, or/and that the second liquid supply is provided with a third one-way valve which is openable only towards the interior of the second liquid supply.
7. The extraction device as claimed in claim 1, further comprising a cavity, wherein the cavity is communicated with the inlet and outlet ends, and a second switch valve for on-off control is arranged between the cavity and the inlet and outlet ends.
8. The extraction apparatus as claimed in claim 1, wherein the power unit is a piston pump, an inlet and an outlet of which are in communication with the extraction chamber; or the power unit and the extraction part form an injector structure, and the power unit is provided with a piston rod matched with the extraction cavity; or the power unit is a dropper, and an inlet and an outlet of the dropper are communicated with the extraction cavity; or the power unit and the extracting piece form a dropper structure, and the power unit is provided with an elastic rubber cap matched with the extracting cavity; and the self-heating unit is used for heating the liquid supply unit and is arranged on the outer side of the liquid supply unit.
9. An in vitro diagnostic apparatus comprising the extraction device according to any one of claims 1 to 8.
10. The in-vitro diagnostic apparatus according to claim 9, further comprising a test paper, wherein a communicating structure is arranged between the sample dripping area of the test paper and the extraction cavity, and the communicating structure is provided with a third switch valve for on-off control.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010378138.0A CN111607483B (en) | 2020-05-07 | 2020-05-07 | In-vitro diagnostic device and extraction device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010378138.0A CN111607483B (en) | 2020-05-07 | 2020-05-07 | In-vitro diagnostic device and extraction device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111607483A true CN111607483A (en) | 2020-09-01 |
| CN111607483B CN111607483B (en) | 2023-09-15 |
Family
ID=72203336
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010378138.0A Active CN111607483B (en) | 2020-05-07 | 2020-05-07 | In-vitro diagnostic device and extraction device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111607483B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114669339A (en) * | 2022-05-27 | 2022-06-28 | 湖南冠牧生物科技有限公司 | Nucleic acid extraction micro-fluidic chip, nucleic acid extraction system and method |
| CN114931986A (en) * | 2022-04-08 | 2022-08-23 | 厦门宝太生物科技股份有限公司 | Nucleic acid detection micro-fluidic device and nucleic acid detection method thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002000255A (en) * | 2000-06-22 | 2002-01-08 | Hitachi Ltd | Apparatus for extracting nucleic acid |
| CN107118961A (en) * | 2017-05-16 | 2017-09-01 | 上海德具生物科技有限公司 | Bacterium or viral online acquisition and on-line automatic detection device in a kind of air |
| CN108102892A (en) * | 2016-11-25 | 2018-06-01 | 苏州百源基因技术有限公司 | Disposable nucleic acid-extracting apparatus |
| CN110272807A (en) * | 2018-03-13 | 2019-09-24 | 武汉医蒂生物科技有限公司 | A kind of nucleic acid extraction purifying positive pressure devices |
| CN110964716A (en) * | 2019-12-05 | 2020-04-07 | 广州万孚生物技术股份有限公司 | In-vitro diagnosis and analysis device and reagent card |
| CN212504816U (en) * | 2020-05-07 | 2021-02-09 | 广州再生医学与健康广东省实验室 | In-vitro diagnostic instrument and extraction device |
-
2020
- 2020-05-07 CN CN202010378138.0A patent/CN111607483B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002000255A (en) * | 2000-06-22 | 2002-01-08 | Hitachi Ltd | Apparatus for extracting nucleic acid |
| CN108102892A (en) * | 2016-11-25 | 2018-06-01 | 苏州百源基因技术有限公司 | Disposable nucleic acid-extracting apparatus |
| CN107118961A (en) * | 2017-05-16 | 2017-09-01 | 上海德具生物科技有限公司 | Bacterium or viral online acquisition and on-line automatic detection device in a kind of air |
| CN110272807A (en) * | 2018-03-13 | 2019-09-24 | 武汉医蒂生物科技有限公司 | A kind of nucleic acid extraction purifying positive pressure devices |
| CN110964716A (en) * | 2019-12-05 | 2020-04-07 | 广州万孚生物技术股份有限公司 | In-vitro diagnosis and analysis device and reagent card |
| CN212504816U (en) * | 2020-05-07 | 2021-02-09 | 广州再生医学与健康广东省实验室 | In-vitro diagnostic instrument and extraction device |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114931986A (en) * | 2022-04-08 | 2022-08-23 | 厦门宝太生物科技股份有限公司 | Nucleic acid detection micro-fluidic device and nucleic acid detection method thereof |
| CN114931986B (en) * | 2022-04-08 | 2022-12-27 | 厦门宝太生物科技股份有限公司 | Nucleic acid detection micro-fluidic device and nucleic acid detection method thereof |
| WO2023193386A1 (en) * | 2022-04-08 | 2023-10-12 | 厦门宝太生物科技股份有限公司 | Nucleic acid detection microfluidic device and nucleic acid detection method therefor |
| CN114669339A (en) * | 2022-05-27 | 2022-06-28 | 湖南冠牧生物科技有限公司 | Nucleic acid extraction micro-fluidic chip, nucleic acid extraction system and method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN111607483B (en) | 2023-09-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US11273443B2 (en) | Devices and methods for molecular diagnostic testing | |
| US8642293B2 (en) | Disposable device for analyzing a liquid sample containing a nucleic acid with a nucleic acid amplification apparatus | |
| CN111655373B (en) | Improved cartridge for in vitro diagnostics and method of use thereof | |
| CN111607483A (en) | In-vitro diagnostic instrument and extraction device | |
| CN109789408A (en) | The system for being used to prepare sample | |
| CN212504816U (en) | In-vitro diagnostic instrument and extraction device | |
| CN217757481U (en) | Reaction assembly | |
| WO2021223184A1 (en) | In-vitro diagnostic instrument and extraction device | |
| CN211877545U (en) | Reagent card, reagent pack and container | |
| CN211871997U (en) | Reagent card and magnetic bead storage structure | |
| JP2022545830A (en) | Filter Devices, Kits, and Methods of Pretreating Samples |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |