CN108102892A - Disposable nucleic acid-extracting apparatus - Google Patents
Disposable nucleic acid-extracting apparatus Download PDFInfo
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- CN108102892A CN108102892A CN201611059328.6A CN201611059328A CN108102892A CN 108102892 A CN108102892 A CN 108102892A CN 201611059328 A CN201611059328 A CN 201611059328A CN 108102892 A CN108102892 A CN 108102892A
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- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
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Abstract
The present invention discloses disposable nucleic acid-extracting apparatus, including imbibition rifle point, cylinder, filter assemblies, barrier assembly and opening device.Imbibition rifle point is sealedly connected on the bottom opening of cylinder；Filter assemblies are arranged on the internal face of cylinder, and cylinder is divided into the first space and second space；Barrier assembly is arranged on the bottom of filter assemblies, and second space is divided into the 3rd cavity positioned at the first cavity, the second cavity and position in the inner along level；3rd cavity is connected with bottom opening；Port is opened up on barrier assembly, the 3rd cavity is connected respectively with the first cavity and the second cavity, port is equipped with sealing element；Two sets of opening devices correspond respectively to the first cavity and the second cavity is arranged on cylinder, for raising or exposing sealing element.The cavity of two cavitys for placing cleaning solution, recovered liquid and sucking nucleic acid is arranged in cylinder so that entire nucleic acid-extracting apparatus is simple in structure, and nucleic acid can be extracted whenever and wherever possible in the wild or in family with meeting people.
Description
Technical field
The present invention relates to the technical fields of nucleic acid, and in particular to a kind of disposable nucleic acid-extracting apparatus.
Background technology
Nucleic acid by many nucleotide polymerizations into large biological molecule compound, be life one of most basic substance, nucleic acid
It is widely present in animal and plant cells, in microbial body, biological nucleic acid in vivo often combines to form nucleoprotein with protein.The prior art
In it is complicated to the apparatus structure of nucleic acid extraction, volume is big, occupied space is big, not readily portable, causes the extraction of nucleic acid only
Can could be used in specific place, greatly be not easy to the use of people, for example, be in or field need extract nucleic acid
When, it can not just carry out.
Chinese patent literature CN106047660A discloses a kind of nucleic acid-extracting apparatus, including tubular body, is arranged on tubulose
Plug in bottom part body opening, the syringe being arranged in tubular body open top, the syringe needle of syringe stretch into tubulose master
The inside of body, the internal face of plug also set up filter core film.
In the handle of operating syringe, nucleic acid solution to be extracted is inhaled through plug for the nucleic acid-extracting apparatus of this structure
Enter the inner cavity of plug and adsorb on filter core film；Solution after filtering enters through filter core film in tubular body, Partial filtration liquid
Enter through syringe needle in the syringe of syringe；The plug of the extraction element is moved on in the container for placing cleaning solution again afterwards, then is inhaled
Enter cleaning solution, washing impurity-removing is carried out to the nucleic acid adsorbed on filter core film, afterwards again moves on to the plug of the nucleic acid-extracting apparatus
In the container for placing recovered liquid, plug sucks recovered liquid again, nucleic acid and filter core UF membrane on filter core film will be adsorbed, after separation
Nucleic acid through plug outlet discharge, complete the extraction of nucleic acid.
But during nucleic acid extraction, the extraction element is constantly moved, plug is allowed to be inserted into different containers,
Mating at least two container is also needed to, to place cleaning solution and recovered liquid respectively, causes the instrument for extracting nucleic acid inconvenient to carry, is used
Also it is inconvenient.
The content of the invention
Therefore, the technical problem to be solved in the present invention is instrument for extracting nucleic acid of the prior art is overcome to need at least to match
Two containers being separately provided cause extraction apparatus carrying and inconvenient problem with use.
For this purpose, the present invention provides a kind of disposable nucleic acid-extracting apparatus, including
Imbibition rifle point；
Cylinder has open top and bottom opening, and one end of the imbibition rifle point is sealedly connected on the bottom opening
On；
Filter assemblies are arranged on the internal face of the cylinder, and vertically the inner cavity of the cylinder is divided into positioned at upper
First space of side and underlying second space have filtering adsorbed film；
Barrier assembly, top are arranged on the bottom of the filter assemblies, and bottom is extended at the bottom opening, along water
It square is divided by the second space positioned at the first cavity of the barrier assembly both sides and the second cavity and positioned at institute
State the 3rd cavity inside barrier assembly；3rd cavity is connected with the bottom opening, the filtering adsorbed film face institute
State the 3rd cavity；
At least two ports are opened up on the barrier assembly, respectively connect the 3rd cavity with first cavity,
It is connected with the 3rd cavity with second cavity, the port top cover labyrinth is equipped with sealing element；And
Opening device is at least two sets, corresponds respectively to first cavity and the second cavity is arranged on the cylinder,
For raising or exposing the sealing element.
Preferably, above-mentioned disposable nucleic acid-extracting apparatus, the opening device include
Driver, drive shaft are stretched into first cavity or the second cavity；
Fixture is fixed in the drive shaft, for clamping the sealing element；Or it is fixed on the point in the drive shaft
Thorn portion, for exposing the sealing element.
Preferably, above-mentioned disposable nucleic acid-extracting apparatus, the barrier assembly include two pieces of vertically arranged isolation boards,
The 3rd cavity is formed between two pieces of isolation boards, the port is opened on the isolation board.
It is further preferred that above-mentioned disposable nucleic acid-extracting apparatus, the port are opened in the close of the isolation board
On side wall at the bottom opening；
The diaphragm seal that the sealing element is formed for tinfoil or elastic material.
It is further preferred that above-mentioned disposable nucleic acid-extracting apparatus, the tinfoil or diaphragm seal are bonded in the port
Outside wall surface on.
Preferably, above-mentioned disposable nucleic acid-extracting apparatus, the filter assemblies further include
The annular mounting plate being fixed in the surface of cylinder's inboard wall, the filtering adsorbed film are fixed on the annular mounting plate
Endoporus on.
Preferably, above-mentioned disposable nucleic acid-extracting apparatus, it is saturating to further include the waterproof being arranged in the surface of cylinder's inboard wall
Air film, the waterproof ventilated membrane are located in first space；
Air extractor is set in the open top, and the air extractor is through the waterproof ventilated membrane to the 3rd cavity
Interior pumping or inflation；
Liquid outlet is opened up on the wall surface between the filter assemblies and the waterproof ventilated membrane of the cylinder, it is described
Removably sealed set cover on liquid outlet.
It is further preferred that above-mentioned disposable nucleic acid-extracting apparatus, the air extractor include
Sealing is fixed on the shell in the open top, and the first vacuum elements being arranged in the shell pass through pipe
The getter case that road is connected with first vacuum elements is connected with the second pumping group on the gas outlet of the getter case by pipeline
Part；
It is connected in the shell with the waterproof ventilated membrane.
It is further preferred that above-mentioned disposable nucleic acid-extracting apparatus, further includes sealing and is fixed in the open top
Head cover, the air extractor is fixed on the head cover, opened up on the head cover air extractor and the waterproof is saturating
The connected entrance of air film connection；
The exhaust pipe of the air intake duct of first vacuum elements and second air extractor, which seals, is threaded through the company
On port.
Preferably, above-mentioned disposable nucleic acid-extracting apparatus, the imbibition rifle are sharp for syringe needle or conical pipe, the conical pipe
Point as suction inlet, the other end seals the bottom opening for being plugged in the cylinder.
Technical solution of the present invention has the following advantages that：
1. disposable nucleic acid-extracting apparatus provided by the invention, including imbibition rifle point, cylinder, filter assemblies, barrier assembly
And at least two sets of opening devices.Wherein, cylinder has open top and bottom opening, and one end of imbibition rifle point is sealedly connected on
On bottom opening；Filter assemblies are arranged on the internal face of cylinder, and the inner cavity of cylinder is vertically divided into be located above
One space and underlying second space have filtering adsorbed film；The bottom of filter assemblies is arranged at the top of barrier assembly
On, bottom is extended at bottom opening, and second space is divided into the first cavity positioned at barrier assembly both sides in the horizontal direction
The 3rd cavity with the second cavity and inside barrier assembly；3rd cavity is connected with bottom opening, and filtering adsorbed film is just
To the 3rd cavity；At least two ports are opened up on barrier assembly, respectively connect the 3rd cavity and the 3rd cavity with the first cavity
It is connected with the second cavity, port top cover labyrinth is equipped with sealing element；And opening device is at least two sets, corresponds respectively to the first chamber
Body and the second cavity are arranged on cylinder, for raising or exposing sealing element.
Second space is divided into three cavitys, the first chamber by the disposable nucleic acid-extracting apparatus of said structure, barrier assembly
Pre-selection is placed with cleaning solution and recovered liquid respectively in body and the second cavity.When being extracted to nucleic acid, nucleic acid solution is first through inhaling
Liquid rifle point is pumped into the 3rd cavity and is attracted on filtering adsorbed film；Afterwards using a set of opening device by the first cavity and the
The sealing element on port between three cavitys is raised or exposed so that the first cavity is connected with the 3rd cavity, then in the first cavity
Cleaning solution be drawn by the port in the 3rd cavity, to filtering adsorbed film on nucleic acid carry out washing impurity-removing；It adopts again afterwards
The sealing element on the port between the 3rd cavity and the second cavity is raised or exposed with another set of opening device, the second cavity with
3rd cavity connects, and the in vivo recovered liquid of the second chamber is drawn into the 3rd cavity, to adsorb the nucleic acid on filtering adsorbed film into
Row departs from and recycles, and the nucleic acid after recycling is excluded through imbibition rifle point, to complete the extraction process of entire nucleic acid so that entire nucleic acid
The structure of extraction element is simply and easy to use, and cleaning solution and recovered liquid are placed respectively without being separately provided multiple containers, with
Meeting people in the wild or in family whenever and wherever possible can extract nucleic acid.
2. disposable nucleic acid-extracting apparatus provided by the invention, opening device includes driver, is fixed on the drive of driver
Fixture or sharp portion on moving axis, the drive shaft of driver are stretched into the first cavity or the second cavity, and fixture is used for grip seal
Part, sharp portion are used to expose sealing element.When needing to connect the first cavity or the second cavity with the 3rd cavity, drive shaft is only needed
It drives fixture to move, to clamp sealing element, sealing element is torn from port；Or directly drive the spine being fixed thereon
Portion is moved towards sealing element, sealing element is exposed, convenient for port is opened.
3. the present invention provides disposable nucleic acid-extracting apparatus, the waterproof and breathable being arranged in the surface of cylinder's inboard wall is further included
Film, waterproof ventilated membrane are located in the first space；Air extractor is set in open top, and air extractor is through waterproof ventilated membrane to the 3rd
Pumping or inflation in cavity；Liquid outlet, liquid outlet are opened up on the wall surface between filter assemblies and waterproof ventilated membrane of cylinder
On removably sealed set cover.Nucleic acid solution is pumped into the 3rd cavity and adsorbs in mistake as power source by air extractor
It filters on adsorbed film, the solution after filtering is discharged through filtering adsorbed film through the liquid outlet on cylinder, and the solution after filtering is avoided to stop
It stays in cylinder, under the action of no air extractor, then reverses through filter adsorbed film outflow, the phenomenon that taking away partial nucleic acid occurs.
Description of the drawings
It, below will be to specific in order to illustrate more clearly of the specific embodiment of the invention or technical solution of the prior art
Embodiment or attached drawing needed to be used in the description of the prior art are briefly described, it should be apparent that, in describing below
Attached drawing is some embodiments of the present invention, for those of ordinary skill in the art, before not making the creative labor
It puts, can also be obtained according to these attached drawings other attached drawings.
Fig. 1 is the schematic diagram of the disposable nucleic acid-extracting apparatus provided in the embodiment of the present invention 1；
Reference sign：1- imbibitions rifle point；2- cylinders；The first spaces of 21-；22- second spaces；23- liquid outlets；3- mistakes
Filter component；31- annular mounting plates；32- filters adsorbed film；4- isolation boards；41- ports；The first cavitys of 42-；The second cavitys of 43-；
The 3rd cavitys of 44-；51- drivers；52- sharp portions；6- sealing elements；7- waterproof ventilated membranes；The first vacuum elements of 81-；82- getters
Case；The second vacuum elements of 83-；84- shells；9- head covers.
Specific embodiment
Technical scheme is clearly and completely described below in conjunction with attached drawing, it is clear that described implementation
Example is part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, ordinary skill
Personnel's all other embodiments obtained without making creative work, belong to the scope of protection of the invention.
In the description of the present invention, it is necessary to explanation, term " " center ", " on ", " under ", "left", "right", " vertical ",
The orientation or position relationship of the instructions such as " level ", " interior ", " outer " be based on orientation shown in the drawings or position relationship, merely to
Convenient for the description present invention and simplify description rather than instruction or imply signified device or element must have specific orientation,
With specific azimuth configuration and operation, therefore it is not considered as limiting the invention.In addition, term " first ", " second ",
" the 3rd " is only used for description purpose, and it is not intended that instruction or hint relative importance.
In the description of the present invention, it is necessary to illustrate, unless otherwise clearly defined and limited, term " installation ", " phase
Even ", " connection " should be interpreted broadly, for example, it may be being fixedly connected or being detachably connected or be integrally connected；It can
To be mechanical connection or be electrically connected；It can be directly connected, can also be indirectly connected by intermediary, Ke Yishi
Connection inside two elements.For the ordinary skill in the art, with concrete condition above-mentioned term can be understood at this
Concrete meaning in invention.
As long as in addition, technical characteristic involved in invention described below different embodiments non-structure each other
It can be combined with each other into conflict.
Embodiment 1
The present embodiment provides a kind of disposable nucleic acid-extracting apparatus, as shown in Figure 1, including syringe needle, cylinder 2, filter assemblies
3rd, barrier assembly, two sets of opening devices and air extractor.
Cylinder 2 has open top and bottom opening, and one end sealing of syringe needle is plugged on bottom opening.Filter assemblies 3
It is inhaled including the annular mounting plate 31 being fixed on 2 internal face of cylinder and the filtering being fixed on the endoporus of annular mounting plate 31
The inner cavity of cylinder 2 is vertically divided into the first space 21 for being located above and underlying by membrane 32, annular mounting plate 31
Second space 22.Sealing is fixed with head cover 9 in the open top of cylinder 2, and connected entrance is opened up on head cover 9, is gone back on 2 internal face of cylinder
Be provided with waterproof ventilated membrane 7, waterproof ventilated membrane 7 is located in the first space 21, cylinder 2 be located at annular mounting plate 31 and waterproof it is saturating
Open up liquid outlet 23 on wall surface between gas film, removably sealed set has cover on liquid outlet 23.
Barrier assembly includes two pieces of vertically arranged isolation boards 4, and the top of isolation board 4 is fixed on the bottom of annular mounting plate 31
On portion surface, bottom is extended at the bottom opening of cylinder 2, and second space 22 is divided into positioned at two isolation in the horizontal direction
The 3rd cavity 44 surrounded between first cavity 42 of 4 both sides of plate and the second cavity 43 and two isolation boards 4；Two isolation
Port 41 is opened up on wall surface at the close bottom opening of plate 4, for respectively will be between the 3rd cavity 44 and the first cavity 42
Connected between connection and the 3rd cavity 44 and the second cavity 43, two ports 41 back on a side surface of the 3rd cavity 44
It is bonded with diaphragm seal.
As shown in Figure 1, air extractor includes the shell 84 being fixed on head cover 9, the first pumping group being fixed on head cover 9
Part 81, the getter case 82 being connected by pipeline with the first vacuum elements 81 are connected with by pipeline on the gas outlet of getter case 82
Second vacuum elements 83.The exhaust pipe of the air intake duct of first vacuum elements 81 and the second vacuum elements 83, which seals, is threaded through head cover
On 9 connected entrance, for pumping in the 3rd cavity 44 or being inflated through waterproof ventilated membrane 7.First vacuum elements 81 and second are taken out
Pneumatic module 83 is both preferably air pump.
Two sets of opening devices are installed on cylinder 2, correspond respectively to the first cavity 42 and the second cavity 43, opening device
It is preferably cylinder or telescope motor including the driver 51 being fixed on cylinder 2, the drive shaft of driver 51 is close in the horizontal direction
Envelope is threaded through on 2 wall surface of cylinder, and stretches into the first cavity 42 or the second cavity 43 and fix sharp portion on the driving shaft
52, under the driving of cylinder, drive shaft drives sharp portion 52 to be moved towards or away from diaphragm seal direction.
Disposable nucleic acid-extracting apparatus in this embodiment, before being extracted to nucleic acid, in nucleic acid-extracting apparatus
The first cavity 42 and the second cavity 43 in fill cleaning solution and recovered liquid respectively, when needing to extract nucleic acid, first
Vacuum elements 81 in the first space 21 to being evacuated, since the first space 21 is only connected with the 3rd cavity 44, then in the 3rd cavity 44
Gas constantly filtered adsorbed film 32, waterproof ventilated membrane 7 into being collected in gas collection case after air intake duct；First
Under the continuous air-extraction function of vacuum elements 81, the gas in the 3rd cavity 44 is constantly taken away, forms subnormal ambient, extraneous
Nucleic acid solution is constantly pumped into the 3rd cavity 44 through syringe needle, has fixed scale on the partition plate wall surface of the 3rd cavity 44, when
When the suction amount of nucleic acid solution reaches this scale, syringe needle leaves the container for holding nucleic acid solution, no longer sucks nucleic acid solution；First
Getter assembly in the 3rd cavity 44 still to applying draft, then nucleic acid is attracted on filtering adsorbed film 32, the solution after filtering into
Enter in the first space 21, when all nucleic acid is all attracted on filtering adsorbed film 32, cylinder 2 is stood upside down placements, the first pumping
Pneumatic module 81 stops applying draft in the 3rd cavity 44, opens the cover on the liquid outlet 23 of 2 wall surface of cylinder at this time, and first is empty
Between solution after filtering in 21 discharged through this liquid outlet 23, complete the first step that nucleic acid is pumped into, then cover sealing cover set
On liquid outlet 23；Cylinder 2 is just being put afterwards, 2 upper cylinder of cylinder is being opened and drives sharp portion 52 towards the diaphragm seal on isolation board 4
Movement, diaphragm seal is poked so that and the first cavity 42 is connected with the 3rd cavity 44, meanwhile, the first vacuum elements 81 are to the 3rd chamber
Apply draft in body 44 again, the cleaning solution in the first cavity 42 is under self gravitation and draft effect, constantly into the 3rd chamber
In body 44, and it is drawn towards filtering adsorbed film 32 and nucleic acid is washed, to remove the impurity carried in nucleic acid, the solution after filtering
It is discharged again through liquid outlet 23；The diaphragm seal on another isolation board 4 is exposed again afterwards, the recovered liquid in the second cavity 43 is taken out
Enter in the 3rd cavity 44, nucleic acid is separated from filtering adsorbed film 32, the solution after filtering is discharged again through liquid outlet 23, is carried
The nucleic acid obtained is by the second pumping combination to inflation in the 3rd cavity 44 so that the pressure in the 3rd cavity 44 is more than the external world
Pressure so as to be discharged through syringe needle, completes the extraction process of nucleic acid, the structure of entire extraction element is simple, more without being separately provided
A container holds cleaning solution and recovered liquid respectively, it becomes possible to nucleic acid be extracted, convenient for outdoors or family is at any time to core
Acid extracts work.Meanwhile the nucleic acid-extracting apparatus is disposable apparatus, prevents nucleic acid-extracting apparatus from reusing, nucleic acid
Cross-infection is between each other so that nucleic acid extraction process is much cleaner.In addition, it is necessary to explanation, above-mentioned waterproof and breathable
Film 7 allows gas by the way that liquid can not pass through.
As interchangeable embodiment, syringe needle can be replaced conical pipe, and the point of conical pipe is another as suction inlet
End sealing is plugged on the bottom opening of cylinder 2.It either can be replaced capillary or imbibition rifle point 1 of the prior art.
As interchangeable embodiment, above-mentioned head cover 9 can also be not provided with, air extractor is directly anchored to cylinder 2
Open top on, the exhaust pipe of the air intake duct of the first vacuum elements 81 and the second vacuum elements 83 stretches into the first space 21
It is interior.
As the deformation of air extractor, air extractor can also be existing other structures, only need that the first sky can be passed through
Between in 21 pair of the 3rd cavity 44 pumping form negative pressure, draft is applied to nucleic acid solution or cleaning solution or recovered liquid；Work as needs
It when discharging the nucleic acid after extraction, can blow through the first space 21 into the 3rd cavity 44, positive pressure be formed, by the nucleic acid after extraction
Discharge.
As the alternative embodiments of waterproof ventilated membrane 7, the installing plate with through hole can also be used and be arranged on
Two sealing plates below installing plate replace, and when being evacuated or inflating, are moved opposite to each other between two sealing plates, to form supplied gas
By gap；It when not being evacuated or inflating, moves toward one another to form sealing surface between two sealing plates so that liquid can not be through
Port 41 on sealing plate and installing plate flows out, and can only be flowed out by liquid outlet 23.
As deformation, above-mentioned waterproof ventilated membrane 7, air extractor and liquid outlet 23 can also be not provided with, passes through other drives
Dynamic structure to be evacuated to be formed subnormal ambient or inflation to the 3rd cavity 44 to form positive pressure environment, is set in driving structure
Solution collecting box is put, the solution filtered after filtered adsorbed film 32 is drawn into this solution collecting box.Or it uses and is arranged on top
Syringe in portion's opening, syringe have two syringe needles stretched into the first space 21, and a syringe needle is used to be evacuated body and liquid
Body, another syringe needle into the first space 21 for inflating, to realize above-mentioned function.
As the deformation of filter assemblies 3, filter assemblies 3 can also be other structures of the prior art, need to only be arranged on
On the internal face of cylinder 2, the inner cavity of cylinder 2 is vertically divided into the first space 21 being located above and underlying second sky
Between 22 and with facing the filtering adsorbed film 32 of the 3rd cavity 44, and cause the first cavity 42 and the second cavity 43 to be close
Seal cavity.It is preferably nylon membrane or existing other filtering adsorbed films in nucleic acid extraction to filter adsorbed film 32
32。
As the deformation of diaphragm seal, sealing ink can also be tinfoil or existing other sealing elements 6, but need resistance to core
The film that the corrosion function of acid solution or prior art elastomeric material are formed, sealing element 6 using bonding mode except being fixed
On port 41, other fixed forms of the prior art can also be used.
As deformation, port 41 can also be opened at the other positions of isolation board 4, only need to be in the effect of vacuum elements
Under, the solution in the first cavity 42 or the second cavity 43 is drawn into through this port 41 in the 3rd cavity 44.As further
Deformation, the sharp portion 52 in above-mentioned opening device may be replaced by fixture, will to the clamping action of sealing element 6 by fixture
Sealing element 6 is raised from port 41.At this point, preferably cylinder is arranged on head cover 9, the annular installation of drive shaft hermetically passing
Plate 31 is vertically stretched into the first cavity 42 or the second cavity 43, drives fixture to the edge of vertically grip seal part 6, thus will
Sealing element 6 tears it down from port 41.As deformation, opening device can also be of the prior art in addition to said structure
Other structures, only two sets of opening devices of need correspond respectively to the first cavity 42 and the second cavity 43 is arranged on cylinder 2, for lifting
It opens or tears sealing element 6 or expose sealing element 6, port 41 is opened.
As the alternative embodiments of barrier assembly, barrier assembly can also be mounted on 31 bottom table of annular mounting plate
Inner cylinder on face is respectively the first cavity 42 and the second cavity 43 positioned at the both sides of inner cylinder, and the inner cavity of inner cylinder is as
Three cavitys 44 open up two ports 41 on the wall surface of inner cylinder, and two ports 41 correspond respectively to the first cavity 42 and the second chamber
Body 43, the top opening size of inner cylinder are consistent with the size for filtering adsorbed film 32.Or other structures of the prior art, only
Top is needed to be arranged on the bottom of filter assemblies 3, bottom is extended at the bottom opening of cylinder 2, empty by second in the horizontal direction
Between 22 be divided into positioned at the first cavity 42 of barrier assembly both sides and the second cavity 43 and inside barrier assembly the 3rd
Cavity 44, the 3rd cavity 44 are connected with bottom opening, the 3rd cavity 44 of 32 face of filtering adsorbed film；Meanwhile the wall of barrier assembly
There are at least two ports 41, each 41 top cover labyrinth of port sets cover on face.
As deformation, the number of port 41 can be three, four, five, six etc., only need to by the first cavity 42 with
3rd cavity 44, the second cavity 43 are connected with the 3rd cavity 44, and the number specifically set is depending on actual use situation.
Accordingly, cover is set on each port 41, and cover is unloaded the mode of being sealedly connected and fixed and consolidated using of the prior art open
It is fixed.
Preferably, there is scale value, convenient for judging sucking 44 kernel acid solution of the 3rd cavity on the wall surface of cylinder 2
Soakage.Or scale value is carved on the vertical wall surface of barrier assembly, convenient for judging nucleic acid solution soakage, cleaning solution sucking
The soakage of amount and recovered liquid.As deformation, the volume of the 3rd cavity 44 can also be arranged to the cavity of fixed volume, only needed
The 3rd cavity 44 is filled, acquiescence is reached to the soakage of nucleic acid, convenient for the extraction work of nucleic acid.
Obviously, the above embodiments are merely examples for clarifying the description, and is not intended to limit the embodiments.It is right
For those of ordinary skill in the art, can also make on the basis of the above description it is other it is various forms of variation or
It changes.There is no necessity and possibility to exhaust all the enbodiments.And the obvious variation thus extended out or
Among changing still in the protection domain of the invention.
Claims (10)
1. disposable nucleic acid-extracting apparatus, which is characterized in that including
Imbibition rifle point (1)；
Cylinder (2), has open top and bottom opening, and one end of the imbibition rifle sharp (1) is sealedly connected on the bottom and opens
On mouth；
Filter assemblies (3), are arranged on the internal face of the cylinder (2), and the inner cavity of the cylinder (2) vertically is divided into position
In the first space (21) of top and underlying second space (22), there is filtering adsorbed film (32)；
Barrier assembly, top are arranged on the bottom of the filter assemblies (3), and bottom is extended at the bottom opening, along water
It square is divided by the second space (22) positioned at the first cavity (42) of the barrier assembly both sides and the second cavity
(43) the 3rd cavity (44) and inside the barrier assembly；3rd cavity (44) connects with the bottom opening
It is logical, the 3rd cavity (44) described in described filtering adsorbed film (32) face；
At least two ports (41) are opened up on the barrier assembly, respectively by the 3rd cavity (44) and first cavity
(42) connection and the 3rd cavity (44) are connected with second cavity (43), and port (41) top cover labyrinth is equipped with sealing element
(6)；And
Opening device is at least two sets, corresponds respectively to first cavity (42) and the second cavity (43) is arranged on the cylinder
On body (2), for raising or exposing the sealing element (6).
2. disposable nucleic acid-extracting apparatus according to claim 1, it is characterised in that：The opening device includes
Driver (51), drive shaft are stretched into first cavity (42) or the second cavity (43)；
Fixture is fixed in the drive shaft, for clamping the sealing element (6)；Or it is fixed on the point in the drive shaft
Thorn portion (52), for exposing the sealing element (6).
3. disposable nucleic acid-extracting apparatus according to claim 1 or 2, it is characterised in that：The barrier assembly includes perpendicular
To two pieces of isolation boards (4) of setting, the 3rd cavity (44), the port (41) are formed between two pieces of isolation boards (4)
It is opened on the isolation board (4).
4. disposable nucleic acid-extracting apparatus according to claim 3, it is characterised in that：The port (41) is opened in described
On side wall at the close bottom opening of isolation board (4)；
The diaphragm seal that the sealing element (6) is formed for tinfoil or elastic material.
5. disposable nucleic acid-extracting apparatus according to claim 4, it is characterised in that：The tinfoil or diaphragm seal are bonded in
In the outside wall surface of the port (41).
6. the disposable nucleic acid-extracting apparatus according to any one of claim 1-5, it is characterised in that：The filter assemblies
(3) further include
The annular mounting plate (31) being fixed on the cylinder (2) internal face, the filtering adsorbed film (32) are fixed on the ring
On the endoporus of shape installing plate (31).
7. the disposable nucleic acid-extracting apparatus according to any one of claim 1-6, it is characterised in that：It further includes and is arranged on
Waterproof ventilated membrane (7) on cylinder (2) internal face, the waterproof ventilated membrane (7) are located in first space (21)；
Air extractor is set in the open top, and the air extractor is through the waterproof ventilated membrane (7) to the 3rd cavity
(44) pumping or inflation in；
Liquid outlet is opened up on the wall surface between the filter assemblies (3) and the waterproof ventilated membrane (7) of the cylinder (2)
(23), removably sealed set cover on the liquid outlet (23).
8. disposable nucleic acid-extracting apparatus according to claim 7, it is characterised in that：The air extractor includes
Sealing is fixed on the shell (84) in the open top, the first vacuum elements being arranged in the shell (84)
(81), the getter case (82) being connected by pipeline with first vacuum elements (81), on the gas outlet of the getter case (82)
Second vacuum elements (83) are connected with by pipeline；
It is connected in the shell (84) with the waterproof ventilated membrane (7).
9. the disposable nucleic acid-extracting apparatus according to any one of claim 1-8, it is characterised in that：It is solid to further include sealing
The head cover (9) being scheduled in the open top, the air extractor are fixed on the head cover (9), are opened up on the head cover (9)
The connected entrance that the air extractor is connected with the waterproof ventilated membrane (7)；
The air intake duct of first vacuum elements (81) and the exhaust pipe of second vacuum elements (83) seal and are threaded through institute
It states on connected entrance.
10. the disposable nucleic acid-extracting apparatus according to any one of claim 1-9, it is characterised in that：The imbibition rifle
Sharp (1) is syringe needle or conical pipe, and for the point of the conical pipe as suction inlet, other end sealing is plugged in the cylinder (2)
Bottom opening.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611059328.6A CN108102892A (en) | 2016-11-25 | 2016-11-25 | Disposable nucleic acid-extracting apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611059328.6A CN108102892A (en) | 2016-11-25 | 2016-11-25 | Disposable nucleic acid-extracting apparatus |
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| Publication Number | Publication Date |
|---|---|
| CN108102892A true CN108102892A (en) | 2018-06-01 |
Family
ID=62205442
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611059328.6A Pending CN108102892A (en) | 2016-11-25 | 2016-11-25 | Disposable nucleic acid-extracting apparatus |
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| Country | Link |
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| CN (1) | CN108102892A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111607483A (en) * | 2020-05-07 | 2020-09-01 | 广州再生医学与健康广东省实验室 | In-vitro diagnostic instrument and extraction device |
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| US9145581B1 (en) * | 2014-10-17 | 2015-09-29 | Daniel Lai | Rapid nucleic acid extraction method and apparatus |
| US20160251628A1 (en) * | 2013-10-30 | 2016-09-01 | Exmoor Pharma Concepts Ltd | Apparatus And Method For Filtration Of A Suspension |
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| US20100236324A1 (en) * | 2007-06-19 | 2010-09-23 | Universal Bio Research Co., Ltd. | Column tip processing device and column tip processing method |
| US20160251628A1 (en) * | 2013-10-30 | 2016-09-01 | Exmoor Pharma Concepts Ltd | Apparatus And Method For Filtration Of A Suspension |
| CN204185484U (en) * | 2014-09-28 | 2015-03-04 | 南京钟鼎生物技术有限公司 | A kind of rapid extraction device for nucleic acid |
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| CN111607483A (en) * | 2020-05-07 | 2020-09-01 | 广州再生医学与健康广东省实验室 | In-vitro diagnostic instrument and extraction device |
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Application publication date: 20180601 |