CN219670500U - POCT microfluidic nucleic acid detection kit - Google Patents
POCT microfluidic nucleic acid detection kit Download PDFInfo
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- CN219670500U CN219670500U CN202320730998.5U CN202320730998U CN219670500U CN 219670500 U CN219670500 U CN 219670500U CN 202320730998 U CN202320730998 U CN 202320730998U CN 219670500 U CN219670500 U CN 219670500U
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- nucleic acid
- poct
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- 238000001514 detection method Methods 0.000 title claims abstract description 37
- 238000012123 point-of-care testing Methods 0.000 title claims abstract description 25
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 23
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 23
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 23
- 239000007788 liquid Substances 0.000 claims abstract description 60
- 238000002347 injection Methods 0.000 claims abstract description 45
- 239000007924 injection Substances 0.000 claims abstract description 45
- 239000003085 diluting agent Substances 0.000 claims abstract description 32
- 238000006243 chemical reaction Methods 0.000 claims abstract description 29
- 239000002699 waste material Substances 0.000 claims abstract description 23
- 238000012360 testing method Methods 0.000 claims abstract description 17
- 238000007789 sealing Methods 0.000 claims abstract description 5
- 210000001503 joint Anatomy 0.000 claims description 6
- 238000007826 nucleic acid assay Methods 0.000 claims 5
- 239000012530 fluid Substances 0.000 claims 1
- 239000000443 aerosol Substances 0.000 abstract description 4
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 3
- 238000004891 communication Methods 0.000 abstract description 3
- 238000005259 measurement Methods 0.000 abstract description 3
- 239000000523 sample Substances 0.000 description 23
- 238000000034 method Methods 0.000 description 4
- 238000000926 separation method Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 238000003032 molecular docking Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 238000012408 PCR amplification Methods 0.000 description 1
- 239000011358 absorbing material Substances 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 239000012470 diluted sample Substances 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000004880 explosion Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
Abstract
The utility model discloses a POCT microfluidic nucleic acid detection kit, which comprises: the microfluidic box comprises a microfluidic box body, wherein a cavity structure and a flow channel structure for communicating all cavities in the cavity structure are arranged on the microfluidic box body, and a cover plate for sealing the cavity structure and the flow channel structure is also arranged on the upper surface of the microfluidic box body; the cavity unit group includes: the reaction tank, the mixing chamber, the waste liquid tank and the test paper placing area are sequentially arranged from the front end to the tail end. The full-sealed cavity structure is realized, the channel structure is utilized for communication, the sample liquid is driven to move sequentially along with the injection of the diluent for detection, and the pollution risks such as aerosol and the like can be avoided in a full-sealed detection environment; the operation mode is simple, the self-driving can be realized only by injecting the diluent after the sample liquid is injected, and the structure is simple; the structure is arranged reasonably, the reagent can be guaranteed to react fully, and the accuracy of the measurement result is guaranteed.
Description
Technical Field
The utility model relates to the technical field of instant inspection equipment, in particular to a POCT (point of care testing) microfluidic nucleic acid detection kit.
Background
In vitro diagnostic (IVD, in Vitro Diagnosis) techniques are used to help determine disease or body function by detecting samples of the body, including blood, body fluid, and tissue, in addition to the human body to obtain relevant clinical diagnostic information. The current IVD technology with greater potential and rapid growth is the molecular diagnostic and point of care (POCT, point of Care Test) technology based on nucleic acid detection.
With the advancement of society, there is an increasing demand for low-cost and safe POCT diagnostic devices that can be used in a use scenario outside of a laboratory, such as in a community hospital, pharmacy, home, etc. At present, most of the nucleic acid detection kits on the market cannot be used in a professional biological laboratory, mainly because sample operation needs to be pollution-proof, the sample operation needs to be completed in the laboratory, and the whole process needs a plurality of professional equipment to complete the cleavage of biological samples, the extraction of nucleic acid molecules, the PCR amplification of nucleic acid and the collection and data analysis of optical signals.
Disclosure of Invention
In view of the above, the utility model provides a POCT microfluidic nucleic acid detection kit, which solves the technical problems that the existing nucleic acid detection kit cannot be fully sealed and has no pollution, and a laboratory is required to be used for sample processing.
The following presents a simplified summary in order to provide a basic understanding of some aspects of the disclosed embodiments. This summary is not an extensive overview and is intended to neither identify key/critical elements nor delineate the scope of such embodiments. Its sole purpose is to present some concepts in a simplified form as a prelude to the more detailed description that is presented later.
The utility model adopts the following technical scheme:
provided is a POCT microfluidic nucleic acid detection kit, comprising: the microfluidic box comprises a microfluidic box body, wherein a cavity structure and a flow channel structure for communicating all cavities in the cavity structure are arranged on the microfluidic box body, and a cover plate for sealing the cavity structure and the flow channel structure is also arranged on the upper surface of the microfluidic box body; the cavity structure comprises: two cavity unit groups arranged in parallel; the cavity unit group includes: and the reaction tank, the mixing chamber, the waste liquid tank and the test paper placing area are sequentially arranged from the front end to the tail end of the microfluidic box main body and are sequentially communicated.
Further, the cavity structure further comprises: a sample liquid injection chamber and a diluent injection chamber; the sample liquid injection cavity and the diluent injection cavity are arranged at the front end of the microfluidic box main body and are communicated with the reaction tank.
Further, the POCT microfluidic nucleic acid detection kit further comprises: the connector is arranged at the front end of the cover plate; a diluent injection port is formed in the connector and is communicated with the diluent injection cavity; and the joint is also provided with a butt joint column shell, and the inner space of the butt joint column shell is communicated with the sample liquid injection cavity.
Further, the POCT microfluidic nucleic acid detection kit further comprises: and the cover plate is adhered to the upper surface of the microfluidic cartridge body through the film.
Further, the cover plate is provided with abdication slotted holes at positions corresponding to the sample liquid injection cavity and the diluent injection cavity respectively.
Further, the cover plate is provided with an observation window at a position corresponding to the test paper placing area.
Further, the flow channel structure includes: i-shaped flow channel; two ends of one side of the I-shaped flow channel are respectively communicated with the sample liquid injection cavity and the diluent injection cavity, and two ends of the other side of the I-shaped flow channel are respectively communicated with the reaction tanks in the two cavity unit groups.
Further, two reaction tanks are arranged in each group of cavity unit groups; the flow channel structure further comprises: t-shaped runner; and two sides of the two reaction tanks are communicated with the mixing chamber and the I-shaped flow channel through the T-shaped flow channels.
Further, a separation sheet is arranged in the waste liquid pool so as to separate the waste liquid pool into a semi-communicated waste liquid collecting cavity and a liquid suction cavity, and the liquid suction cavity is positioned at one side close to the test paper placing area.
Further, the flow channel structure further includes: and one end of the single flow passage is communicated with the mixing chamber, and the other end of the single flow passage is connected to one side of the waste liquid pool where the liquid suction cavity is located.
The utility model has the beneficial effects that:
1. the structure arrangement of the utility model realizes the full sealing of the cavity structure and utilizes the communication of the flow channel structure, the sample liquid is driven to move sequentially for detection along with the injection of the diluent, and the pollution risks such as aerosol and the like can be avoided in the full-closed detection environment;
2. the operation mode is simple, the self-driving can be realized only by injecting the diluent after the sample liquid is injected, and the structure is simple;
3. the structure is arranged reasonably, the reagent can be guaranteed to react fully, and the accuracy of the measurement result is guaranteed.
Drawings
In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, it being obvious that the drawings in the following description are only some embodiments of the utility model, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of an explosion structure of a POCT microfluidic nucleic acid detection kit of the present utility model;
fig. 2 is a diagram showing the structure of the upper surface of the body of the microfluidic cartridge according to the present utility model.
Detailed Description
Embodiments of the present utility model will be described in detail below with reference to the accompanying drawings. It should be understood that the described embodiments are merely some, but not all, embodiments of the utility model. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
As shown in fig. 1-2, in some illustrative embodiments, the present utility model provides a POCT microfluidic nucleic acid detection kit comprising: a microfluidic cartridge body 1, a cover plate 2, a connector 3 and a film 4.
The microfluidic box body 1 is provided with a cavity structure and a flow channel structure, each cavity in the cavity structure is used for providing space for each detection step, and the flow channel structure is used for communicating each cavity in the cavity structure so as to realize sequential flow of detection liquid.
The cover plate 2 is arranged on the upper surface of the microfluidic box main body 1, and seals the cavity structure and the flow channel structure, so that a closed system is formed between each cavity and each flow channel, and the fully-closed detection environment can avoid pollution risks such as aerosol. The cover plate 2 is adhered to the upper surface of the microfluidic box main body 1 through the film 4, the outline shape of the film 4 is consistent with that of the cover plate 2, firstly, the assembly mode is simple, secondly, the structure is stable after adhesion, and good sealing performance can be ensured.
The cavity structure includes: the sample liquid injection cavity 9, the diluent injection cavity 10 and two cavity unit groups which are arranged on the upper surface of the microfluidic cartridge main body 1 in parallel. Each cavity unit group comprises: reaction tank 5, mixing chamber 6, waste liquid pond 7 and test paper place district 8, and reaction tank 5, mixing chamber 6, waste liquid pond 7 and test paper place district 8 and arrange in proper order from the front end of microfluidic cartridge main part 1 to terminal, communicate in proper order according to the liquid flow direction simultaneously. Wherein, the sample liquid injection cavity 9 and the diluent injection cavity 10 are arranged at the front end of the microfluidic box body 1 and are communicated with the reaction tank 5.
The reaction tank 5 is positioned at the front section of the microfluidic box main body 1, and freeze-dried balls are placed in the reaction tank in advance, so that the sample to be detected can fully react and amplify in the chamber. Preferably, each group of cavity unit group is internally provided with two parallel reaction tanks 5, so that the reaction is ensured to be full, and the accuracy of the measurement result is further ensured.
The mixing chamber 6 is positioned at the middle section of the microfluidic cartridge body 1 and is used for sufficiently diluting the sample to be detected so as to achieve the degree of meeting the chromatographic reaction. The test paper placing area 8 is positioned at the tail section of the microfluidic box main body 1 and is used for placing detection test paper. The waste liquid pools 7 are positioned at two sides of the front end of the test paper placing area 8 and are used for collecting redundant detection samples.
A connector 3 is provided on the upper surface of the front end of the cover plate 2 for providing an operative interface for the patient. The connector 3 is provided with a diluent injection port 301, and when the cover plate 2 is adhered to the microfluidic cartridge body 1, the diluent injection port 301 is communicated with the diluent injection cavity 10. The joint 3 is further provided with a docking post housing 302, and when the cover plate 2 is adhered to the microfluidic cartridge body 1, the internal space of the docking post housing 302 communicates with the sample liquid injection chamber 9. Therefore, in actual operation, the patient only needs to inject the sample to be detected into the microfluidic cartridge main body 1 through the butt joint column casing 302, and then inject the diluent through the diluent injection opening 301, so that the operation mode is simple, and pollution in the operation process can be avoided.
In order to ensure smooth injection of the liquid, the cover plate 2 is provided with relief slots 201 at positions corresponding to the sample liquid injection cavity 9 and the diluent liquid injection cavity 10, respectively. Further, the cover plate 2 is provided with an observation window 202 at a position corresponding to the test paper placing area 8, so that the patient can observe the detection result conveniently.
The runner structure includes: an I-shaped runner 101, a T-shaped runner 102 and a single runner 103.
Two ends of one side of the I-shaped flow channel 101 are respectively communicated with the sample liquid injection cavity 9 and the diluent injection cavity 10, and two ends of the other side of the I-shaped flow channel 101 are respectively communicated with the reaction tanks 5 in the two groups of cavity unit groups, so that liquid in the sample liquid injection cavity 9 and the diluent injection cavity 10 can uniformly enter the two groups of cavity unit groups, and the reaction is ensured to be sufficient.
Two sides of the two reaction tanks 5 in each cavity unit group are respectively communicated with the mixing chamber 6 and the I-shaped flow channel 101 through the T-shaped flow channels 102, so that the liquid in the sample liquid injection cavity 9 and the diluent injection cavity 10 can uniformly enter the two reaction tanks 5 in the two cavity unit groups, and the reaction is ensured to be full.
The waste liquid pool 7 is internally provided with a separation sheet 104 to separate the waste liquid pool 7 into a waste liquid collecting cavity 701 and a liquid absorbing cavity 702, and the separation sheet 104 does not completely block the waste liquid pool 7, i.e. the waste liquid collecting cavity 701 and the liquid absorbing cavity 702 are in a semi-communication state. The pipette cavity 702 is located on the side near the test strip placement area 8 for ease of detection. One end of the single flow channel 103 is communicated with the mixing chamber 6, and the other end of the single flow channel is connected to one side of the waste liquid tank 7 where the liquid suction chamber 702 is located, so that liquid discharged from the mixing chamber 6 firstly enters the liquid suction chamber 702 to contact with test paper, and the redundant liquid is discharged into the waste liquid tank 7.
The test flow is as follows:
firstly, a sample to be detected is injected into a sample liquid injection cavity 9 through a butt joint column shell 302 on a joint 3, finally is gathered into a reaction tank 5, fully reacts with freeze-dried balls reserved in the reaction tank 5 and completes amplification;
then, diluent is injected into the diluent injection cavity 10 through the diluent injection opening 301, the diluent enters the reaction tank 5, the amplified sample to be detected is sufficiently diluted in the reaction tank 5, and the redundant diluted sample to be detected enters the mixing chamber 6 through the flow channel structure;
in the above process, as the diluent is injected, the more the liquid in the mixing chamber 6 will gather, and after the mixing chamber 6 is filled, the diluted product further enters the liquid absorbing chamber 702, and the excessive liquid will enter the waste liquid collecting chamber 701, and the water absorbing material such as water absorbing sponge can be placed in the waste liquid tank 7 in advance.
Finally, the test strip starts chromatography to finish the subsequent color reaction.
The POCT microfluidic nucleic acid detection kit is an integral body which is not divided, and is discarded by one person, so that detection equipment is abandoned, and the pollution of a detection device to a reagent card is prevented. Meanwhile, the integrated nucleic acid detection box can be completely separated from a nucleic acid detection device in a laboratory, so that the full-closed detection is realized, and the pollution risks such as aerosol and the like are completely eradicated. And the full automation of detection is realized, no external power is needed, so that the complexity of the detection device is reduced, and the operation is concise.
The foregoing is merely illustrative of the present utility model, and the present utility model is not limited thereto, and any changes or substitutions easily contemplated by those skilled in the art within the scope of the present utility model should be included in the present utility model. Therefore, the protection scope of the utility model is subject to the protection scope of the claims.
Claims (10)
1. A POCT microfluidic nucleic acid detection kit, comprising: the microfluidic box comprises a microfluidic box body, wherein a cavity structure and a flow channel structure for communicating all cavities in the cavity structure are arranged on the microfluidic box body, and a cover plate for sealing the cavity structure and the flow channel structure is also arranged on the upper surface of the microfluidic box body; the cavity structure comprises: two cavity unit groups arranged in parallel; the cavity unit group includes: and the reaction tank, the mixing chamber, the waste liquid tank and the test paper placing area are sequentially arranged from the front end to the tail end of the microfluidic box main body and are sequentially communicated.
2. The POCT microfluidic nucleic acid detection kit of claim 1, wherein the cavity structure further comprises: a sample liquid injection chamber and a diluent injection chamber; the sample liquid injection cavity and the diluent injection cavity are arranged at the front end of the microfluidic box main body and are communicated with the reaction tank.
3. The POCT microfluidic nucleic acid detection kit of claim 2, further comprising: the connector is arranged at the front end of the cover plate; a diluent injection port is formed in the connector and is communicated with the diluent injection cavity; and the joint is also provided with a butt joint column shell, and the inner space of the butt joint column shell is communicated with the sample liquid injection cavity.
4. A POCT microfluidic nucleic acid assay kit of claim 3, further comprising: and the cover plate is adhered to the upper surface of the microfluidic cartridge body through the film.
5. The POCT microfluidic nucleic acid assay kit of claim 4, wherein the cover plate is provided with a relief slot at a location corresponding to the sample fluid injection cavity and the diluent injection cavity, respectively.
6. The POCT microfluidic nucleic acid assay kit of claim 5, wherein the cover sheet is provided with an observation window at a position corresponding to the test strip placement area.
7. The POCT microfluidic nucleic acid assay kit of claim 6, wherein the flow channel structure comprises: i-shaped flow channel; two ends of one side of the I-shaped flow channel are respectively communicated with the sample liquid injection cavity and the diluent injection cavity, and two ends of the other side of the I-shaped flow channel are respectively communicated with the reaction tanks in the two cavity unit groups.
8. The POCT microfluidic nucleic acid detection kit according to claim 7, wherein two reaction tanks are arranged in each cavity unit group; the flow channel structure further comprises: t-shaped runner; and two sides of the two reaction tanks are communicated with the mixing chamber and the I-shaped flow channel through the T-shaped flow channels.
9. The POCT microfluidic nucleic acid assay kit of claim 8, wherein a separator is disposed in the waste reservoir to separate the waste reservoir into a semi-connected waste collection chamber and a pipetting chamber, the pipetting chamber being located on a side adjacent to the test strip placement region.
10. The POCT microfluidic nucleic acid detection kit of claim 9, wherein the flow channel structure further comprises: and one end of the single flow passage is communicated with the mixing chamber, and the other end of the single flow passage is connected to one side of the waste liquid pool where the liquid suction cavity is located.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320730998.5U CN219670500U (en) | 2023-04-06 | 2023-04-06 | POCT microfluidic nucleic acid detection kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320730998.5U CN219670500U (en) | 2023-04-06 | 2023-04-06 | POCT microfluidic nucleic acid detection kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219670500U true CN219670500U (en) | 2023-09-12 |
Family
ID=87892879
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202320730998.5U Active CN219670500U (en) | 2023-04-06 | 2023-04-06 | POCT microfluidic nucleic acid detection kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219670500U (en) |
-
2023
- 2023-04-06 CN CN202320730998.5U patent/CN219670500U/en active Active
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