CN113286892A - 一种利用囊胚培养液检测胚胎健康状况的方法和产品 - Google Patents
一种利用囊胚培养液检测胚胎健康状况的方法和产品 Download PDFInfo
- Publication number
- CN113286892A CN113286892A CN201980068400.7A CN201980068400A CN113286892A CN 113286892 A CN113286892 A CN 113286892A CN 201980068400 A CN201980068400 A CN 201980068400A CN 113286892 A CN113286892 A CN 113286892A
- Authority
- CN
- China
- Prior art keywords
- blastocyst
- culture
- embryo
- cultured
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000002459 blastocyst Anatomy 0.000 title claims abstract description 228
- 210000001161 mammalian embryo Anatomy 0.000 title claims abstract description 105
- 238000000034 method Methods 0.000 title claims abstract description 80
- 230000036541 health Effects 0.000 title claims abstract description 38
- 238000001514 detection method Methods 0.000 claims abstract description 82
- 238000000338 in vitro Methods 0.000 claims abstract description 44
- 238000012360 testing method Methods 0.000 claims abstract description 34
- 230000008859 change Effects 0.000 claims abstract description 14
- 230000002068 genetic effect Effects 0.000 claims abstract description 8
- 238000005070 sampling Methods 0.000 claims description 30
- 210000000349 chromosome Anatomy 0.000 claims description 18
- 108090000623 proteins and genes Proteins 0.000 claims description 17
- 238000012258 culturing Methods 0.000 claims description 14
- 238000003776 cleavage reaction Methods 0.000 claims description 12
- 230000007017 scission Effects 0.000 claims description 12
- 239000012530 fluid Substances 0.000 claims description 11
- 239000007788 liquid Substances 0.000 claims description 8
- 238000011156 evaluation Methods 0.000 claims description 7
- 210000004340 zona pellucida Anatomy 0.000 claims description 6
- 238000012545 processing Methods 0.000 claims description 4
- 210000000625 blastula Anatomy 0.000 claims description 3
- 238000003745 diagnosis Methods 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 claims 1
- 239000012531 culture fluid Substances 0.000 abstract description 21
- 230000008774 maternal effect Effects 0.000 abstract description 14
- 230000008569 process Effects 0.000 abstract description 7
- 239000000243 solution Substances 0.000 description 92
- 239000000523 sample Substances 0.000 description 63
- 210000002257 embryonic structure Anatomy 0.000 description 35
- 239000002609 medium Substances 0.000 description 23
- 230000004720 fertilization Effects 0.000 description 18
- 210000004027 cell Anatomy 0.000 description 15
- 230000003862 health status Effects 0.000 description 13
- 238000011534 incubation Methods 0.000 description 13
- 238000000137 annealing Methods 0.000 description 12
- 230000003321 amplification Effects 0.000 description 11
- 239000001963 growth medium Substances 0.000 description 11
- 238000003199 nucleic acid amplification method Methods 0.000 description 11
- 102000053602 DNA Human genes 0.000 description 10
- 108020004414 DNA Proteins 0.000 description 10
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 9
- 239000000203 mixture Substances 0.000 description 9
- 230000009089 cytolysis Effects 0.000 description 8
- 235000013601 eggs Nutrition 0.000 description 8
- 238000011331 genomic analysis Methods 0.000 description 8
- 102000039446 nucleic acids Human genes 0.000 description 8
- 108020004707 nucleic acids Proteins 0.000 description 8
- 150000007523 nucleic acids Chemical class 0.000 description 8
- 238000012163 sequencing technique Methods 0.000 description 8
- 102000004317 Lyases Human genes 0.000 description 7
- 108090000856 Lyases Proteins 0.000 description 7
- 238000004458 analytical method Methods 0.000 description 7
- 230000013020 embryo development Effects 0.000 description 7
- 238000007710 freezing Methods 0.000 description 7
- 230000008014 freezing Effects 0.000 description 7
- 102000002322 Egg Proteins Human genes 0.000 description 5
- 108010000912 Egg Proteins Proteins 0.000 description 5
- 230000008901 benefit Effects 0.000 description 5
- 210000004952 blastocoel Anatomy 0.000 description 5
- 238000013412 genome amplification Methods 0.000 description 5
- 239000006166 lysate Substances 0.000 description 5
- 210000004681 ovum Anatomy 0.000 description 5
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 4
- 239000007983 Tris buffer Substances 0.000 description 4
- 239000013504 Triton X-100 Substances 0.000 description 4
- 229920004890 Triton X-100 Polymers 0.000 description 4
- 230000002159 abnormal effect Effects 0.000 description 4
- 230000037416 cystogenesis Effects 0.000 description 4
- 238000004925 denaturation Methods 0.000 description 4
- 230000036425 denaturation Effects 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 239000012634 fragment Substances 0.000 description 4
- 238000002156 mixing Methods 0.000 description 4
- 210000000472 morula Anatomy 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 4
- QCQCHGYLTSGIGX-GHXANHINSA-N 4-[[(3ar,5ar,5br,7ar,9s,11ar,11br,13as)-5a,5b,8,8,11a-pentamethyl-3a-[(5-methylpyridine-3-carbonyl)amino]-2-oxo-1-propan-2-yl-4,5,6,7,7a,9,10,11,11b,12,13,13a-dodecahydro-3h-cyclopenta[a]chrysen-9-yl]oxy]-2,2-dimethyl-4-oxobutanoic acid Chemical compound N([C@@]12CC[C@@]3(C)[C@]4(C)CC[C@H]5C(C)(C)[C@@H](OC(=O)CC(C)(C)C(O)=O)CC[C@]5(C)[C@H]4CC[C@@H]3C1=C(C(C2)=O)C(C)C)C(=O)C1=CN=CC(C)=C1 QCQCHGYLTSGIGX-GHXANHINSA-N 0.000 description 3
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 3
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 208000036878 aneuploidy Diseases 0.000 description 3
- 231100001075 aneuploidy Toxicity 0.000 description 3
- 238000001574 biopsy Methods 0.000 description 3
- 239000002738 chelating agent Substances 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 238000005553 drilling Methods 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- 239000012139 lysis buffer Substances 0.000 description 3
- 230000002438 mitochondrial effect Effects 0.000 description 3
- 239000002736 nonionic surfactant Substances 0.000 description 3
- 210000000287 oocyte Anatomy 0.000 description 3
- 230000001717 pathogenic effect Effects 0.000 description 3
- 239000011148 porous material Substances 0.000 description 3
- 238000004080 punching Methods 0.000 description 3
- 230000002829 reductive effect Effects 0.000 description 3
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- 101000925662 Enterobacteria phage PRD1 Endolysin Proteins 0.000 description 2
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 2
- 235000008708 Morus alba Nutrition 0.000 description 2
- 240000000249 Morus alba Species 0.000 description 2
- 239000004365 Protease Substances 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 108010006785 Taq Polymerase Proteins 0.000 description 2
- 230000002411 adverse Effects 0.000 description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 2
- 235000011130 ammonium sulphate Nutrition 0.000 description 2
- 230000006727 cell loss Effects 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 238000007429 general method Methods 0.000 description 2
- 238000010166 immunofluorescence Methods 0.000 description 2
- 230000000415 inactivating effect Effects 0.000 description 2
- 230000002779 inactivation Effects 0.000 description 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 2
- 235000019341 magnesium sulphate Nutrition 0.000 description 2
- 238000004949 mass spectrometry Methods 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 238000000520 microinjection Methods 0.000 description 2
- 230000009525 mild injury Effects 0.000 description 2
- 230000008775 paternal effect Effects 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 235000002020 sage Nutrition 0.000 description 2
- 210000003765 sex chromosome Anatomy 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000007671 third-generation sequencing Methods 0.000 description 2
- 238000002054 transplantation Methods 0.000 description 2
- 210000004291 uterus Anatomy 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- IDOQDZANRZQBTP-UHFFFAOYSA-N 2-[2-(2,4,4-trimethylpentan-2-yl)phenoxy]ethanol Chemical compound CC(C)(C)CC(C)(C)C1=CC=CC=C1OCCO IDOQDZANRZQBTP-UHFFFAOYSA-N 0.000 description 1
- 102100027211 Albumin Human genes 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- KWIUHFFTVRNATP-UHFFFAOYSA-N Betaine Natural products C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 description 1
- 208000031404 Chromosome Aberrations Diseases 0.000 description 1
- 108010017826 DNA Polymerase I Proteins 0.000 description 1
- 102000004594 DNA Polymerase I Human genes 0.000 description 1
- 108010067770 Endopeptidase K Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 108010078851 HIV Reverse Transcriptase Proteins 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 102100034343 Integrase Human genes 0.000 description 1
- 241000713869 Moloney murine leukemia virus Species 0.000 description 1
- 108010014251 Muramidase Proteins 0.000 description 1
- 102000016943 Muramidase Human genes 0.000 description 1
- KWIUHFFTVRNATP-UHFFFAOYSA-O N,N,N-trimethylglycinium Chemical compound C[N+](C)(C)CC(O)=O KWIUHFFTVRNATP-UHFFFAOYSA-O 0.000 description 1
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 1
- 108090000526 Papain Proteins 0.000 description 1
- 102000057297 Pepsin A Human genes 0.000 description 1
- 108090000284 Pepsin A Proteins 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 108010001244 Tli polymerase Proteins 0.000 description 1
- 229920004929 Triton X-114 Polymers 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 230000005856 abnormality Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 229960003237 betaine Drugs 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000029803 blastocyst development Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000002759 chromosomal effect Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000005138 cryopreservation Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 210000003754 fetus Anatomy 0.000 description 1
- 238000010448 genetic screening Methods 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 210000004565 granule cell Anatomy 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000002934 lysing effect Effects 0.000 description 1
- 229960000274 lysozyme Drugs 0.000 description 1
- 239000004325 lysozyme Substances 0.000 description 1
- 235000010335 lysozyme Nutrition 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 229940055729 papain Drugs 0.000 description 1
- 235000019834 papain Nutrition 0.000 description 1
- 229940111202 pepsin Drugs 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 235000019419 proteases Nutrition 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 210000002993 trophoblast Anatomy 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 229960001322 trypsin Drugs 0.000 description 1
- 238000004017 vitrification Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M43/00—Combinations of bioreactors or fermenters with other apparatus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0604—Whole embryos; Culture medium therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Gynecology & Obstetrics (AREA)
- Reproductive Health (AREA)
- Developmental Biology & Embryology (AREA)
- Cell Biology (AREA)
- Sustainable Development (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
提供了一种利用囊胚培养液检测胚胎健康状况的方法和产品。具体地，提供了一种体外利用囊胚培养液检测胚胎健康状况的方法，其特征在于，包括步骤：(a)提供第一囊胚培养体系，所述第一囊胚培养体系含有体外培养天数为D5‑D6的囊胚；(b)将所述囊胚转移到含新鲜囊胚培养液的第二囊胚培养体系中进行换液培养，所述换液培养的时间为T1，从而获得经培养的囊胚培养液；(c)将所述经培养的囊胚培养液取出，从而获得无细胞的经培养的囊胚培养液，即为检测样本；和(d)对所述检测样本进行基因检测，从而鉴定所述囊胚的健康状况。该方法可去除IVF和ICSI技术过程中多余精子和母源颗粒细胞污染干扰的风险，并可精确的鉴定胚胎的健康状况。
Description
PCT国内申请，说明书已公开。
Claims (12)
- PCT国内申请，权利要求书已公开。
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811647225.0A CN109536581B (zh) | 2018-12-29 | 2018-12-29 | 一种利用囊胚培养液检测胚胎健康状况的方法和产品 |
| CN2018116472250 | 2018-12-29 | ||
| PCT/CN2019/129196 WO2020135713A1 (zh) | 2018-12-29 | 2019-12-27 | 一种利用囊胚培养液检测胚胎健康状况的方法和产品 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113286892A true CN113286892A (zh) | 2021-08-20 |
| CN113286892B CN113286892B (zh) | 2024-02-23 |
Family
ID=65831526
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811647225.0A Active CN109536581B (zh) | 2018-12-29 | 2018-12-29 | 一种利用囊胚培养液检测胚胎健康状况的方法和产品 |
| CN201980068400.7A Active CN113286892B (zh) | 2018-12-29 | 2019-12-27 | 一种利用囊胚培养液检测胚胎健康状况的方法和产品 |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811647225.0A Active CN109536581B (zh) | 2018-12-29 | 2018-12-29 | 一种利用囊胚培养液检测胚胎健康状况的方法和产品 |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US20220112549A1 (zh) |
| EP (1) | EP3904529A1 (zh) |
| JP (1) | JP2022516543A (zh) |
| CN (2) | CN109536581B (zh) |
| WO (1) | WO2020135713A1 (zh) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114752552A (zh) * | 2022-05-07 | 2022-07-15 | 序康医疗科技（苏州）有限公司 | 冷冻胚胎的复苏、培养、优选和单囊胚移植方法以及用于复苏冷冻胚胎的无创检测方法 |
| CN115678964A (zh) * | 2022-11-08 | 2023-02-03 | 广州女娲生命科技有限公司 | 基于胚胎培养液的植入前胚胎的无创筛选方法 |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109536581B (zh) * | 2018-12-29 | 2022-02-01 | 序康医疗科技（苏州）有限公司 | 一种利用囊胚培养液检测胚胎健康状况的方法和产品 |
| CN111440857B (zh) * | 2020-03-11 | 2023-03-21 | 阿吉安(福州)基因医学检验实验室有限公司 | 用于无创胚胎植入前遗传性检测的方法 |
| CN112582022B (zh) * | 2020-07-21 | 2021-11-23 | 序康医疗科技（苏州）有限公司 | 用于无创胚胎移植优先级评级的系统和方法 |
| CN113862303A (zh) * | 2020-08-24 | 2021-12-31 | 北京希诺谷生物科技有限公司 | 利用体细胞克隆制备克隆马胚胎的方法 |
| CN114107182A (zh) * | 2022-01-07 | 2022-03-01 | 徐维海 | 一种提高囊胚形成率的方法 |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080085836A1 (en) * | 2006-09-22 | 2008-04-10 | Kearns William G | Method for genetic testing of human embryos for chromosome abnormalities, segregating genetic disorders with or without a known mutation and mitochondrial disorders following in vitro fertilization (IVF), embryo culture and embryo biopsy |
| CN105368936A (zh) * | 2015-11-05 | 2016-03-02 | 上海序康医疗科技有限公司 | 一种利用囊胚培养液检测胚胎染色体异常的方法 |
| CN106086199A (zh) * | 2016-07-05 | 2016-11-09 | 上海序康医疗科技有限公司 | 一种利用不含透明带的囊胚培养液检测胚胎染色体异常的方法 |
| CN107760773A (zh) * | 2017-10-26 | 2018-03-06 | 北京中仪康卫医疗器械有限公司 | 一种对胚胎培养液进行scRRBS分析的方法 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009059139A1 (en) * | 2007-10-31 | 2009-05-07 | Christopher William Lipari | Assay of nitric oxide metabolites in the culture media of human preimplantation embryos |
| CN103890191B (zh) | 2011-05-27 | 2018-12-04 | 哈佛大学校长及研究员协会 | 单细胞全基因组扩增方法 |
| CN105722995A (zh) * | 2013-06-18 | 2016-06-29 | 国家医疗保健研究所 | 用于测定胚胎质量的方法 |
| EP3099818B1 (en) * | 2014-01-30 | 2020-03-18 | Pécsi Tudományegyetem | Preimplantation assessment of embryos through detection of free embryonic dna |
| CN104711362A (zh) * | 2015-03-27 | 2015-06-17 | 苏州贝康医疗器械有限公司 | 一种利用囊胚期胚胎细胞进行胚胎染色体异常检测的方法 |
| CN108641929A (zh) * | 2018-02-23 | 2018-10-12 | 深圳市瀚海基因生物科技有限公司 | 确定胚胎是否存在异常的方法和系统 |
| CN109536581B (zh) * | 2018-12-29 | 2022-02-01 | 序康医疗科技（苏州）有限公司 | 一种利用囊胚培养液检测胚胎健康状况的方法和产品 |
-
2018
- 2018-12-29 CN CN201811647225.0A patent/CN109536581B/zh active Active
-
2019
- 2019-12-27 CN CN201980068400.7A patent/CN113286892B/zh active Active
- 2019-12-27 WO PCT/CN2019/129196 patent/WO2020135713A1/zh active Application Filing
- 2019-12-27 JP JP2021538406A patent/JP2022516543A/ja not_active Withdrawn
- 2019-12-27 EP EP19903766.4A patent/EP3904529A1/en not_active Withdrawn
- 2019-12-27 US US17/418,934 patent/US20220112549A1/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080085836A1 (en) * | 2006-09-22 | 2008-04-10 | Kearns William G | Method for genetic testing of human embryos for chromosome abnormalities, segregating genetic disorders with or without a known mutation and mitochondrial disorders following in vitro fertilization (IVF), embryo culture and embryo biopsy |
| CN105368936A (zh) * | 2015-11-05 | 2016-03-02 | 上海序康医疗科技有限公司 | 一种利用囊胚培养液检测胚胎染色体异常的方法 |
| CN106086199A (zh) * | 2016-07-05 | 2016-11-09 | 上海序康医疗科技有限公司 | 一种利用不含透明带的囊胚培养液检测胚胎染色体异常的方法 |
| CN107760773A (zh) * | 2017-10-26 | 2018-03-06 | 北京中仪康卫医疗器械有限公司 | 一种对胚胎培养液进行scRRBS分析的方法 |
Non-Patent Citations (1)
| Title |
|---|
| HAITAO WU等: "Medium-Based Noninvasive Preimplantation Genetic Diagnosis for Human α-Thalassemias-SEA" * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114752552A (zh) * | 2022-05-07 | 2022-07-15 | 序康医疗科技（苏州）有限公司 | 冷冻胚胎的复苏、培养、优选和单囊胚移植方法以及用于复苏冷冻胚胎的无创检测方法 |
| CN115678964A (zh) * | 2022-11-08 | 2023-02-03 | 广州女娲生命科技有限公司 | 基于胚胎培养液的植入前胚胎的无创筛选方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109536581A (zh) | 2019-03-29 |
| JP2022516543A (ja) | 2022-02-28 |
| US20220112549A1 (en) | 2022-04-14 |
| CN109536581B (zh) | 2022-02-01 |
| WO2020135713A1 (zh) | 2020-07-02 |
| CN113286892B (zh) | 2024-02-23 |
| EP3904529A1 (en) | 2021-11-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109536581B (zh) | 一种利用囊胚培养液检测胚胎健康状况的方法和产品 | |
| AU2016351034B2 (en) | Method of detecting chromosome abnormality in embryo by using blastocyst culture | |
| Leaver et al. | Non-invasive preimplantation genetic testing (niPGT): the next revolution in reproductive genetics? | |
| Los et al. | The development of cytogenetically normal, abnormal and mosaic embryos: a theoretical model | |
| US20080085836A1 (en) | Method for genetic testing of human embryos for chromosome abnormalities, segregating genetic disorders with or without a known mutation and mitochondrial disorders following in vitro fertilization (IVF), embryo culture and embryo biopsy | |
| Braude et al. | Preimplantation genetic diagnosis | |
| Brouillet et al. | Is cell-free DNA in spent embryo culture medium an alternative to embryo biopsy for preimplantation genetic testing? A systematic review | |
| US20170044610A1 (en) | Compositions and methods for genetic analysis of embryos | |
| Cimadomo et al. | The dawn of the future: 30 years from the first biopsy of a human embryo. The detailed history of an ongoing revolution | |
| Takeuchi | Pre‐implantation genetic testing: past, present, future | |
| WO2018006706A1 (zh) | 一种利用不含透明带的囊胚培养液检测胚胎染色体异常的方法 | |
| EP3099818A1 (en) | Preimplantation assessment of embryos through detection of free embryonic dna | |
| Yang et al. | Presence of embryonic DNA in culture medium | |
| Shamash et al. | Preimplantation genetic haplotyping a new application for diagnosis of translocation carrier’s embryos-preliminary observations of two robertsonian translocation carrier families | |
| CN111440857B (zh) | 用于无创胚胎植入前遗传性检测的方法 | |
| Gavrilov et al. | Non-viable human embryos as a source of viable cells for embryonic stem cell derivation | |
| CN116515965A (zh) | 一种筛选具有优良发育潜能胚胎的无创检测方法 | |
| Emiliani et al. | Comparison of the validity of preimplantation genetic diagnosis for embryo chromosomal anomalies by fluorescence in situ hybridization on one or two blastomeres | |
| Finanger | Non-Invasive Methods for Detection of Genetic Abnormalities in Human Embryos Cell-Free DNA Secreted from Human Embryos as an Alternative to DNA from Cell Biopsies for Genetic Analyses | |
| West | The use of DNA probes in preimplantation and prenatal diagnosis | |
| Vaccari | Non-Invasive Preimplantation Genetic Testing: a Laboratory Review: NIPGTA-a laboratory review | |
| Gosden et al. | Micromanipulation in assisted reproductive technology: intracytoplasmic sperm injection, assisted hatching, and preimplantation genetic diagnosis | |
| Li et al. | Whether the log-likelihood ratio-based IVF-PGTA assay is a more efficient method? | |
| Karami et al. | Comparing the advantages, disadvantages and diagnostic power of different non-invasive pre-implantation genetic testing: A literature review | |
| Simpson et al. | Overview and Historical Perspective of Preimplantation Genetic Testing |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |