CN113208944A - Skin care composition and skin care product with moisturizing effect - Google Patents
Skin care composition and skin care product with moisturizing effect Download PDFInfo
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- CN113208944A CN113208944A CN202110534602.5A CN202110534602A CN113208944A CN 113208944 A CN113208944 A CN 113208944A CN 202110534602 A CN202110534602 A CN 202110534602A CN 113208944 A CN113208944 A CN 113208944A
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- polysaccharide extract
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- crude polysaccharide
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- 241000608170 Gymnadenia conopsea Species 0.000 claims abstract description 78
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- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 173
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- IKIIZLYTISPENI-ZFORQUDYSA-N baicalin Chemical compound O1[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1OC(C(=C1O)O)=CC2=C1C(=O)C=C(C=1C=CC=CC=1)O2 IKIIZLYTISPENI-ZFORQUDYSA-N 0.000 claims description 30
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- AQHDANHUMGXSJZ-UHFFFAOYSA-N baicalin Natural products OC1C(O)C(C(O)CO)OC1OC(C(=C1O)O)=CC2=C1C(=O)C=C(C=1C=CC=CC=1)O2 AQHDANHUMGXSJZ-UHFFFAOYSA-N 0.000 claims description 30
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- 235000019410 glycyrrhizin Nutrition 0.000 claims description 30
- LPLVUJXQOOQHMX-QWBHMCJMSA-N glycyrrhizinic acid Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@H](O[C@@H]1O[C@@H]1C([C@H]2[C@]([C@@H]3[C@@]([C@@]4(CC[C@@]5(C)CC[C@@](C)(C[C@H]5C4=CC3=O)C(O)=O)C)(C)CC2)(C)CC1)(C)C)C(O)=O)[C@@H]1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O LPLVUJXQOOQHMX-QWBHMCJMSA-N 0.000 claims description 30
- 235000011187 glycerol Nutrition 0.000 claims description 29
- KWIUHFFTVRNATP-UHFFFAOYSA-N glycine betaine Chemical compound C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 claims description 29
- 241001313855 Bletilla Species 0.000 claims description 24
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/345—Alcohols containing more than one hydroxy group
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/44—Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
- A61K8/602—Glycosides, e.g. rutin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/63—Steroids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Emergency Medicine (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Sustainable Development (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Dermatology (AREA)
- Cosmetics (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to a skin care composition and a skin care product with moisturizing effect. The skin care composition with the moisturizing effect comprises the following components in parts by weight: 1 to 5 parts of gymnadenia conopsea polysaccharide extract, 1 to 5 parts of bletilla striata polysaccharide extract and 2 to 8 parts of astragalus polysaccharide extract. The skin care composition has excellent moisturizing effect.
Description
Technical Field
The invention relates to the technical field of skin care products, in particular to a skin care composition with a moisturizing effect and a skin care product.
Background
Moisture is the basis of skin metabolism, skin needs to be nourished by water, and various skin problems such as dryness, dullness, roughness and the like can occur when the skin lacks water. Modern office workers are in a closed air-conditioned room space for a long time, skin moisture is accelerated to evaporate, the skin moisture is physiologically reduced due to the increase of age, and external environmental factors such as the change of autumn and winter have great influence on the skin, so that the skin moisturizing and moisturizing has great crowd demands.
Bletilla striata, a dried tuber of Bletilla striata (Bletilla (Thunb.) Reichb. f.) belonging to the family Orchidaceae. As a Chinese medicinal material, has effects of astringing to stop bleeding, detumescence and promoting granulation, and can be used for treating hemoptysis, hematemesis, traumatic hemorrhage, pyocutaneous disease and toxic swelling, chapped skin, etc. In the traditional method, bletilla striata polysaccharide can be prepared by water extraction and alcohol precipitation of bletilla striata, and research and verification prove that the bletilla striata polysaccharide has moisture absorption and moisture retention performances and can be used for moisturizing and preserving moisture of skin.
In addition, most skin care products on the market contain a plurality of complex components, the raw materials from chemical synthesis are abundant, and additives such as preservatives, essences, pigments and the like are used in a plurality of ways, so that the skin burden can be increased by the components, and the moisturizing effect is opposite.
Disclosure of Invention
Based on this, there is a need for a skin care composition with moisturizing effect. The skin care composition has excellent moisturizing effect.
According to one aspect of the invention, the skin care composition with the moisturizing effect comprises the following components in parts by weight: 1 to 5 parts of gymnadenia conopsea crude polysaccharide extract, 1 to 5 parts of bletilla striata crude polysaccharide extract and 2 to 8 parts of astragalus membranaceus crude polysaccharide extract.
In one embodiment, the skin care composition with moisturizing effect comprises the following components in parts by weight: 2 to 3 parts of gymnadenia conopsea crude polysaccharide extract, 2 to 3 parts of bletilla striata crude polysaccharide extract and 5 to 6 parts of astragalus membranaceus crude polysaccharide extract.
In one embodiment, the skin care composition with moisturizing effect comprises the following components in parts by weight: 2.3 to 2.7 parts of gymnadenia conopsea crude polysaccharide extract, 2.5 to 3 parts of bletilla striata crude polysaccharide extract and 5 to 5.5 parts of astragalus membranaceus crude polysaccharide extract.
In one embodiment, the preparation method of the gymnadenia conopsea crude polysaccharide extract comprises the following steps:
extracting rhizoma Gymnadeniae decoction pieces with water, concentrating the extractive solution, and preparing into concentrated solution;
adding ethanol into the concentrated solution to ensure that the volume concentration of the ethanol in the solution system is 50-80%, standing the obtained solution system at the temperature of 1-10 ℃, and collecting solids.
In one embodiment, the water-adding extraction refers to adding water to carry out boiling-maintaining extraction twice, the first time is adding water with the weight 25-40 times of that of the gymnadenia conopsea piece to carry out water extraction for 1.5-3 hours, and the second time is adding water with the weight 15-30 times of that of the gymnadenia conopsea piece to carry out water extraction for 1-1.5 hours.
In one embodiment, the preparation method of the bletilla crude polysaccharide extract comprises the following steps:
extracting rhizoma bletilla decoction pieces with water, concentrating the extractive solution, and making into concentrated solution;
adding ethanol into the concentrated solution to ensure that the volume concentration of the ethanol in the solution system is 50-80%, standing the obtained solution system at the temperature of 1-10 ℃, and collecting solids.
In one embodiment, the water-adding extraction refers to adding water to perform boiling-maintaining extraction twice, the first time is to add water which is 20-35 times of the weight of the bletilla striata decoction pieces to perform extraction for 1.5-3 hours, and the second time is to add water which is 15-30 times of the weight of the bletilla striata decoction pieces to perform extraction for 1-1.5 hours.
In one embodiment, the preparation method of the astragalus crude polysaccharide extract comprises the following steps:
extracting radix astragali decoction pieces with water, concentrating the obtained extractive solution, and preparing into concentrated solution;
adding ethanol into the concentrated solution to ensure that the volume concentration of the ethanol in the solution system is 50-80%, standing the obtained solution system at the temperature of 1-10 ℃, and collecting solids.
In one embodiment, the water-adding extraction refers to adding water to preserve boiling and extracting twice, the water which is 10-20 times of the weight of the astragalus membranaceus decoction pieces is added for the first time to extract for 1-2 hours, and the water which is 10-15 times of the weight of the bletilla striata decoction pieces is added for the second time to extract for 0.5-1 hour.
In another aspect of the invention, a skin care product is provided, which comprises the skin care composition with moisturizing effect, and a skin care substrate.
In one embodiment, the skin care base comprises at least one of glycerin, trimethylglycine, baicalin, glycyrrhizin, and asiaticoside.
In one embodiment, the skin care product comprises the following components in parts by weight:
1 to 5 parts of gymnadenia conopsea crude polysaccharide extract, 1 to 5 parts of bletilla striata crude polysaccharide extract, 2 to 8 parts of astragalus membranaceus crude polysaccharide extract, 0.1 to 10 parts of glycerol, 0.1 to 5 parts of trimethylglycine, 0.01 to 3 parts of baicalin, 0.01 to 5 parts of glycyrrhizin, 0.01 to 5 parts of asiaticoside and 0 to 70 parts of water.
Compared with the prior art, the invention has the following beneficial effects:
according to the invention, researches show that the water replenishing and moisturizing performances of the coarse bletilla polysaccharide extract can be obviously improved by compounding the coarse gymnadenia conopsea polysaccharide extract, the coarse astragalus polysaccharide extract and the coarse bletilla polysaccharide extract, and one of the three is not necessary, so that the water replenishing and moisturizing performances superior to those of two compounded extracts are obtained through mutual cooperation. Meanwhile, the skin care composition has a simple formula, all the components are from plant components, so that the stimulation of chemical synthesis components is avoided, the skin comfort can be improved, the skin burden is light, and the use is safe.
Detailed Description
In order that the invention may be more readily understood, reference will now be made to the following more particular description of the invention, examples of which are set forth below. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. These embodiments are provided so that this disclosure will be thorough and complete.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.
The term "crude gymnadenia conopsea polysaccharide extract" as used herein refers to an extract containing gymnadenia conopsea polysaccharide compounds, and it is understood that the extract may contain other active ingredients besides gymnadenia conopsea polysaccharide compounds, and is not particularly limited. The content of the crude polysaccharide extract of gymnadenia conopsea prepared in the examples can be determined by conventional methods such as ultraviolet spectrophotometer. The same applies to the bletilla striata crude polysaccharide extract and the astragalus membranaceus crude polysaccharide extract.
Specifically, in the examples of the present invention and the comparative examples:
the content of crude polysaccharide in the gymnadenia conopsea crude polysaccharide extract is determined to be in the range of 85.154-92.729% by adopting a phenol-sulfuric acid method, and the content of protein in the gymnadenia conopsea crude polysaccharide extract is determined to be in the range of 1.165-1.862% by adopting a Kjeldahl method;
the content of crude polysaccharide in the bletilla crude polysaccharide extract is determined to be within the range of 65.215-74.697% by adopting a phenol-sulfuric acid method, and the content of protein in the bletilla crude polysaccharide extract is determined to be within the range of 3.497-4.185% by adopting a Kjeldahl method;
the content of crude polysaccharide in the astragalus crude polysaccharide extract is determined to be within the range of 68.317-75.532% by adopting a phenol-sulfuric acid method, and the content of protein in the astragalus crude polysaccharide extract is determined to be within the range of 14.682-19.532% by adopting a Kjeldahl method.
The boiling-maintaining method is characterized in that after water is boiled, the water is kept in a slightly boiling state, and the temperature is controlled to be 95-100 ℃.
The invention provides a skin care composition with a moisturizing effect, which comprises the following components in parts by weight: 1 to 5 parts of gymnadenia conopsea crude polysaccharide extract, 1 to 5 parts of bletilla striata crude polysaccharide extract and 2 to 8 parts of astragalus membranaceus crude polysaccharide extract.
Generally, plant polysaccharides have the effects of regulating immunity, resisting aging, resisting tumors and the like. Astragalus membranaceus, also known as astragalus membranaceus. The astragalus root has been used for more than 2000 years, and has the functions of enhancing the immunologic function of organisms, protecting the liver, promoting urination, resisting aging, resisting stress, reducing blood pressure and having wider antibacterial action. In the traditional method, astragalus polysaccharide can be prepared by extracting, concentrating and purifying dried roots of astragalus, and research proves that the astragalus polysaccharide can be used as an immune promoter or regulator and has the effects of resisting virus, tumors, aging, radiation, stress, oxidation and the like. Conic gymnadenia rhizome, also called conic gymnadenia rhizome, is a perennial herb of gymnadenia genus of orchidaceae family, is a commonly used traditional Chinese medicine because the tuber of the conic gymnadenia rhizome is shaped like a palm, and can be used for treating diseases such as weakness after illness, cough, asthma, chronic diarrhea, blood loss, hepatitis, brucellosis and the like by being used alone or in combination with other traditional Chinese medicines. In the traditional method, the gymnadenia conopsea polysaccharide can be prepared by the procedures of crushing, degreasing by petroleum ether, removing impurities by ethanol, extracting with water and precipitating with ethanol, removing protein, precipitating with ethanol, washing and the like. The research on the action of the gymnadenia conopsea polysaccharide mainly focuses on the aspects of delaying senility, regulating immunity, relieving fatigue, resisting anoxia and the like.
In the process of carrying out systematic research on plant polysaccharide, the invention discovers that compounding the gymnadenia conopsea crude polysaccharide extract, the astragalus root crude polysaccharide extract and the bletilla striata crude polysaccharide extract can obviously improve the water replenishing and moisturizing performances of the bletilla striata crude polysaccharide extract. Meanwhile, tests prove that the gymnadenia conopsea crude polysaccharide extract, the astragalus root crude polysaccharide extract and the bletilla striata crude polysaccharide extract have a synergistic interaction effect, but the water replenishing and moisturizing performances which can be achieved by the synergistic interaction of the gymnadenia conopsea crude polysaccharide extract, the astragalus root crude polysaccharide extract and the bletilla striata crude polysaccharide extract are obviously higher than those of any two of the complex combinations. Meanwhile, the skin care composition has the advantages of simple formula, easiness in preparation and low cost, all the components are from plant components, the stimulation of chemical synthesis components is avoided, the skin comfort can be improved, the skin burden is light, and the use is safe.
In one specific example, the skin care composition with moisturizing effect comprises the following components in parts by weight: 2 to 3 parts of gymnadenia conopsea crude polysaccharide extract, 2 to 3 parts of bletilla striata crude polysaccharide extract and 5 to 6 parts of astragalus membranaceus crude polysaccharide extract.
In one specific example, the skin care composition with moisturizing effect comprises the following components in parts by weight: 2.3 to 2.7 parts of gymnadenia conopsea crude polysaccharide extract, 2.5 to 3 parts of bletilla striata crude polysaccharide extract and 5 to 5.5 parts of astragalus membranaceus crude polysaccharide extract.
Furthermore, the extraction processes of the gymnadenia conopsea crude polysaccharide extract, the bletilla striata crude polysaccharide extract and the astragalus membranaceus crude polysaccharide extract, particularly the volume concentration of ethanol and the standing temperature in a system are reasonably controlled, so that the moisture retention property of the prepared composition can be optimized.
In one specific example, the preparation method of the gymnadenia conopsea crude polysaccharide extract comprises the following steps:
extracting rhizoma Gymnadeniae decoction pieces with water, concentrating the extractive solution, and preparing into concentrated solution;
adding ethanol into the concentrated solution to ensure that the volume concentration of the ethanol in the solution system is 50-80%, standing the obtained solution system at the temperature of 1-10 ℃, and collecting the solid.
In one specific example, the volume concentration of ethanol in the solution system is selected from a concentration interval consisting of any one or two of the following concentrations: 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%.
In one specific example, the obtained solution system can be left to stand under any one of the following temperature conditions or a temperature interval consisting of any two temperatures: 1 deg.C, 2 deg.C, 3 deg.C, 4 deg.C, 5 deg.C, 6 deg.C, 7 deg.C, 8 deg.C, 9 deg.C, 10 deg.C.
In one specific example, before water extraction, the gymnadenia conopsea decoction pieces are crushed into 70-90 meshes. For example, 70 mesh, 73 mesh, 75 mesh, 76 mesh, 77 mesh, 78 mesh, 79 mesh, 80 mesh, 81 mesh, 82 mesh, 83 mesh, 84 mesh, 85 mesh, 87 mesh, 90 mesh.
In one specific example, ethanol is added to the concentrated solution to make the volume concentration of the ethanol in the solution system be 55-70%. Further, ethanol is added into the concentrated solution to ensure that the volume concentration of the ethanol in the solution system is 55-65%.
In one specific example, the resulting solution system is allowed to stand at 1 ℃ to 5 ℃.
In one specific example, the water-adding extraction refers to water-adding boiling-maintaining extraction twice, the first time is to add water with the weight 25-40 times of that of the gymnadenia conopsea piece for 1.5-3 hours, and the second time is to add water with the weight 15-30 times of that of the gymnadenia conopsea piece for 1-1.5 hours.
In one specific example, the extracting solution is prepared by adding water to keep boiling and extracting the extracting solution twice, sieving the extracting solution with a 300-400-mesh sieve and then mixing the extracting solution. For example, a 300 mesh sieve, a 350 mesh sieve and a 400 mesh sieve.
In one specific example, concentrating refers to concentrating the extract to a solid content (in weight percent) of 2% to 10%.
In some embodiments, the solids content is selected from the following range of values consisting of any one or two of the following weight percentages: 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%.
In one specific example, the standing time is 12 to 24 hours. Examples of rest times include, but are not limited to: 12 hours, 14 hours, 15 hours, 16 hours, 18 hours, 20 hours, 21 hours, 22 hours, 24 hours.
In one specific example, the step of collecting the solid refers to centrifuging at the rotating speed of 3000 r/min-5000 r/min for 5 minutes-15 minutes, then removing the supernatant, taking the precipitate, and drying at the temperature of 50-60 ℃ until the moisture (mass fraction) is less than 10%.
Examples of rotational speeds for centrifugal operation include, but are not limited to: 3000r/min, 3500r/min, 4000r/min, 4500r/min and 5000 r/min.
Examples of centrifugation times include, but are not limited to: 5 minutes, 6 minutes, 7 minutes, 8 minutes, 9 minutes, 10 minutes, 11 minutes, 12 minutes, 13 minutes, 14 minutes, 15 minutes.
Examples of temperature conditions for drying include, but are not limited to: 50 ℃, 51 ℃, 52 ℃, 53 ℃, 54 ℃, 55 ℃, 56 ℃, 57 ℃, 58 ℃, 59 ℃ and 60 ℃.
In one specific example, the preparation method of the bletilla striata crude polysaccharide extract comprises the following steps:
extracting rhizoma bletilla decoction pieces with water, concentrating the extractive solution, and making into concentrated solution;
adding ethanol into the concentrated solution to ensure that the volume concentration of the ethanol in the solution system is 50-80%, standing the obtained solution system at the temperature of 1-10 ℃, and collecting solids.
In one specific example, the volume concentration of ethanol in the solution system is selected from a concentration interval consisting of any one or two of the following concentrations: 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%.
In one specific example, the obtained solution system can be left to stand under any one of the following temperature conditions or a temperature interval consisting of any two temperatures: 1 deg.C, 2 deg.C, 3 deg.C, 4 deg.C, 5 deg.C, 6 deg.C, 7 deg.C, 8 deg.C, 9 deg.C, 10 deg.C.
In one specific example, the bletilla striata decoction pieces are ground to 30-50 meshes before being extracted by adding water. For example, 30 mesh, 33 mesh, 35 mesh, 36 mesh, 37 mesh, 38 mesh, 39 mesh, 40 mesh, 41 mesh, 42 mesh, 43 mesh, 44 mesh, 45 mesh, 47 mesh, 50 mesh.
In one specific example, ethanol is added to the concentrated solution to make the volume concentration of the ethanol in the solution system be 55-70%. Further, ethanol is added into the concentrated solution to ensure that the volume concentration of the ethanol in the solution system is 55-65%.
In one specific example, the resulting solution system is allowed to stand at 1 ℃ to 5 ℃.
In one specific example, the water-adding extraction refers to adding water to perform boiling-maintaining extraction twice, the first time is adding water 20-35 times the weight of the bletilla striata decoction pieces to perform water extraction for 1.5-3 hours, and the second time is adding water 15-30 times the weight of the bletilla striata decoction pieces to perform water extraction for 1-1.5 hours.
In one specific example, the extracting solution is prepared by adding water to keep boiling and extracting the extracting solution twice, sieving the extracting solution with a 300-400-mesh sieve and then mixing the extracting solution. For example, a 300 mesh sieve, a 350 mesh sieve and a 400 mesh sieve.
In one specific example, concentrating refers to concentrating the extract to a solid content (in weight percent) of 2% to 10%.
In some embodiments, the solids content is selected from the following range of values consisting of any one or two of the following weight percentages: 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%.
In one specific example, the standing time is 12 to 24 hours. Examples of rest times include, but are not limited to: 12 hours, 14 hours, 15 hours, 16 hours, 18 hours, 20 hours, 21 hours, 22 hours, 24 hours.
In one specific example, the step of collecting the solid refers to centrifuging at the rotating speed of 3000 r/min-5000 r/min for 5 minutes-15 minutes, then removing the supernatant, taking the precipitate, and drying at the temperature of 50-60 ℃ until the moisture (mass fraction) is less than 10%.
Examples of rotational speeds for centrifugal operation include, but are not limited to: 3000r/min, 3500r/min, 4000r/min, 4500r/min and 5000 r/min.
Examples of centrifugation times include, but are not limited to: 5 minutes, 6 minutes, 7 minutes, 8 minutes, 9 minutes, 10 minutes, 11 minutes, 12 minutes, 13 minutes, 14 minutes, 15 minutes.
Examples of temperature conditions for drying include, but are not limited to: 50 ℃, 51 ℃, 52 ℃, 53 ℃, 54 ℃, 55 ℃, 56 ℃, 57 ℃, 58 ℃, 59 ℃ and 60 ℃.
In one specific example, the preparation method of the astragalus crude polysaccharide extract comprises the following steps:
extracting radix astragali decoction pieces with water, concentrating the obtained extractive solution, and preparing into concentrated solution;
adding ethanol into the concentrated solution to ensure that the volume concentration of the ethanol in the solution system is 50-80%, standing the obtained solution system at the temperature of 1-10 ℃, and collecting the solid.
In one specific example, the volume concentration of ethanol in the solution system is selected from a concentration interval consisting of any one or two of the following concentrations: 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%.
In one specific example, the obtained solution system can be left to stand under any one of the following temperature conditions or a temperature interval consisting of any two temperatures: 1 deg.C, 2 deg.C, 3 deg.C, 4 deg.C, 5 deg.C, 6 deg.C, 7 deg.C, 8 deg.C, 9 deg.C, 10 deg.C.
In one specific example, the astragalus decoction pieces are cut into 3 mm-10 mm slices before being extracted by adding water.
In one specific example, ethanol is added to the concentrated solution to make the volume concentration of the ethanol in the solution system be 55-70%. Further, ethanol is added into the concentrated solution to ensure that the volume concentration of the ethanol in the solution system is 55-65%.
In one specific example, the resulting solution system is allowed to stand at 1 ℃ to 5 ℃.
In one specific example, the water-adding extraction refers to adding water to preserve boiling and extracting twice, wherein the water with the weight 10-20 times of that of the astragalus membranaceus decoction pieces is added for the first time for 1-2 hours, and the water with the weight 10-15 times of that of the astragalus membranaceus decoction pieces is added for the second time for 0.5-1 hour.
In one specific example, the extracting solution is prepared by adding water to keep boiling and extracting the extracting solution twice, sieving the extracting solution with a 300-400-mesh sieve and then mixing the extracting solution. For example, a 300 mesh sieve, a 350 mesh sieve and a 400 mesh sieve.
In one specific example, concentrating refers to concentrating the extract to a solid content (in weight percent) of 30-50%.
In some embodiments, the solids content is selected from the following range of values consisting of any one or two of the following weight percentages: 30%, 33%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 47%, 50%.
In one specific example, the standing time is 12 to 24 hours. Examples of rest times include, but are not limited to: 12 hours, 14 hours, 15 hours, 16 hours, 18 hours, 20 hours, 21 hours, 22 hours, 24 hours.
In one specific example, the step of collecting the solid refers to centrifuging at the rotating speed of 3000 r/min-5000 r/min for 5 minutes-15 minutes, then removing the supernatant, taking the precipitate, and drying at the temperature of 50-60 ℃ until the moisture (mass fraction) is less than 10%.
Examples of rotational speeds for centrifugal operation include, but are not limited to: 3000r/min, 3500r/min, 4000r/min, 4500r/min and 5000 r/min.
Examples of centrifugation times include, but are not limited to: 5 minutes, 6 minutes, 7 minutes, 8 minutes, 9 minutes, 10 minutes, 11 minutes, 12 minutes, 13 minutes, 14 minutes, 15 minutes.
Examples of temperature conditions for drying include, but are not limited to: 50 ℃, 51 ℃, 52 ℃, 53 ℃, 54 ℃, 55 ℃, 56 ℃, 57 ℃, 58 ℃, 59 ℃ and 60 ℃.
The invention also provides a skin care product which comprises the skin care composition with the moisturizing effect and a skin care substrate.
In the further research process of the invention, the skin care composition is compounded with the amino acid humectant with a natural structure, so that the moisture retention of the skin can be rapidly improved, the moisture can be firmly locked, the cell activity can be excited, and the absorption of the skin to natural anti-inflammatory active ingredients such as baicalin, glycyrrhizin, asiaticoside and the like can be improved, thereby playing a role in diminishing inflammation and relieving itching, and improving the anti-allergy capability and the repair capability of the skin.
In one specific example, the skin care base is selected from at least one of glycerin, trimethylglycine, baicalin, glycyrrhizin, and asiaticoside.
In one specific example, the skin care product comprises the following components in parts by weight:
1 to 5 parts of gymnadenia conopsea crude polysaccharide extract, 1 to 5 parts of bletilla striata crude polysaccharide extract, 2 to 8 parts of astragalus membranaceus crude polysaccharide extract, 0.1 to 10 parts of glycerol, 0.1 to 5 parts of trimethylglycine, 0.01 to 3 parts of baicalin, 0.01 to 5 parts of glycyrrhizin, 0.01 to 5 parts of asiaticoside and 0 to 70 parts of water.
In one specific example, the skin care product comprises the following components in parts by weight:
1 to 5 parts of gymnadenia conopsea crude polysaccharide extract, 1 to 5 parts of bletilla striata crude polysaccharide extract, 2 to 8 parts of astragalus membranaceus crude polysaccharide extract, 5 to 10 parts of glycerol, 0.1 to 1 part of trimethylglycine, 0.05 to 0.5 part of baicalin, 0.01 to 0.3 part of glycyrrhizin, 0.1 to 0.5 part of asiaticoside and 60 to 70 parts of water.
In one specific example, the skin care product comprises the following components in parts by weight:
2 to 3 parts of gymnadenia conopsea crude polysaccharide extract, 2 to 3 parts of bletilla striata crude polysaccharide extract, 5 to 6 parts of astragalus membranaceus crude polysaccharide extract, 5 to 10 parts of glycerol, 0.1 to 1 part of trimethylglycine, 0.05 to 0.5 part of baicalin, 0.01 to 0.3 part of glycyrrhizin, 0.1 to 0.5 part of asiaticoside and 60 to 70 parts of water.
In one specific example, the skin care product comprises the following components in parts by weight:
2.3 to 2.7 parts of gymnadenia conopsea crude polysaccharide extract, 2.5 to 3 parts of bletilla striata crude polysaccharide extract, 5 to 5.5 parts of astragalus membranaceus crude polysaccharide extract, 5 to 10 parts of glycerol, 0.1 to 1 part of trimethylglycine, 0.05 to 0.5 part of baicalin, 0.01 to 0.3 part of glycyrrhizin, 0.1 to 0.5 part of asiaticoside and 60 to 70 parts of water.
Hereinafter, specific examples will be described, and the raw materials used in the examples are all commercially available products unless otherwise specified.
Example 1
The embodiment is a skin care product with moisturizing effect and capable of improving skin comfort, and the skin care product comprises the following raw materials in parts by weight:
2.5 parts of gymnadenia conopsea crude polysaccharide extract, 3 parts of bletilla striata crude polysaccharide extract, 5 parts of astragalus membranaceus crude polysaccharide extract, 5 parts of glycerol, 0.2 part of trimethylglycine, 0.08 part of baicalin, 0.1 part of glycyrrhizin, 0.2 part of asiaticoside and 60 parts of deionized water.
The preparation method comprises the following steps:
(a) preparing a gymnadenia conopsea crude polysaccharide extract:
taking gymnadenia conopsea decoction pieces, crushing into 80-mesh fine powder, adding water for boiling-maintaining extraction for 2 times, adding 40 times of water for extraction for 2.5 hours for the first time, and adding 20 times of water for extraction for 1 hour for the second time; respectively sieving the two extracting solutions with a 350-mesh sieve, mixing the extracting solutions, and concentrating until the solid content is 4%; adding 95 vol% ethanol into the concentrated solution until the volume concentration of ethanol in the solution system is 60%, standing in a refrigerator at 4 deg.C for 16 hr, centrifuging at 4000r/min for 10 min, removing supernatant, oven drying the precipitate at 50 deg.C until the water content is less than 10%, and pulverizing to 80 mesh to obtain rhizoma Gymnadeniae crude polysaccharide extract.
(b) Preparing a bletilla striata crude polysaccharide extract:
pulverizing rhizoma bletilla decoction pieces into 40 mesh fine powder, adding water, boiling and extracting for 2 times, adding 30 times of water for the first time, extracting for 3 hr, adding 20 times of water for the second time, and extracting for 1.5 hr; respectively sieving the two extracting solutions with a 350-mesh sieve, mixing the extracting solutions, and concentrating until the solid content is 3%; adding 95 vol% ethanol into the concentrated solution until the volume concentration of ethanol in the solution system is 60%, standing in a refrigerator at 4 deg.C for 16 hr, centrifuging at 4000r/min for 10 min, removing supernatant, oven drying the precipitate at 50 deg.C until the water content is less than 10%, and pulverizing to 80 mesh to obtain rhizoma bletilla crude polysaccharide extract.
(c) Preparing the astragalus crude polysaccharide extract:
cutting radix astragali decoction pieces into 5mm pieces, adding water, boiling for 2 times, extracting for 1.5 hr with 20 times of water for the first time, and extracting for 0.5 hr with 12 times of water for the second time; respectively sieving the two extracting solutions with 350 mesh sieve, mixing the extracting solutions, and concentrating until the solid content is 40%; adding 95 vol% ethanol into the concentrated solution until the volume concentration of ethanol in the solution system is 60%, standing in a refrigerator at 4 deg.C for 16 hr, centrifuging at 4000r/min for 10 min, removing supernatant, oven drying the precipitate at 50 deg.C until the water content is less than 10%, and pulverizing to 80 mesh to obtain crude radix astragali polysaccharide extract.
(d) Taking glycerol according to the weight parts, adding the gymnadenia conopsea crude polysaccharide extract, the bletilla striata crude polysaccharide extract and the astragalus membranaceus crude polysaccharide extract, and uniformly stirring;
(e) adding trimethylglycine, baicalin, glycyrrhizin, asiaticoside and deionized water into the mixture obtained in the step (d) according to the weight parts;
(f) passing the mixture of step (e) through a 200 mesh screen;
(g) UV sterilization for 50 minutes.
Example 2
The embodiment is a skin care product with moisturizing effect and capable of improving skin comfort, and the difference between the raw material formula and the preparation method in embodiment 1 is as follows: the weight parts of the gymnadenia conopsea crude polysaccharide extract, the bletilla striata crude polysaccharide extract and the astragalus membranaceus crude polysaccharide extract are different.
The specific scheme is as follows:
the raw material formula comprises the following raw materials in parts by weight:
3 parts of gymnadenia conopsea crude polysaccharide extract, 2 parts of bletilla striata crude polysaccharide extract, 6 parts of astragalus membranaceus crude polysaccharide extract, 5 parts of glycerol, 0.2 part of trimethylglycine, 0.08 part of baicalin, 0.1 part of glycyrrhizin, 0.2 part of asiaticoside and 60 parts of deionized water.
The preparation method comprises the following steps:
(a) preparing a gymnadenia conopsea crude polysaccharide extract:
taking gymnadenia conopsea decoction pieces, crushing into 80-mesh fine powder, adding water for boiling-maintaining extraction for 2 times, adding 40 times of water for extraction for 2.5 hours for the first time, and adding 20 times of water for extraction for 1 hour for the second time; respectively sieving the two extracting solutions with a 350-mesh sieve, mixing the extracting solutions, and concentrating until the solid content is 4%; adding 95 vol% ethanol into the concentrated solution until the volume concentration of ethanol in the solution system is 60%, standing in a refrigerator at 4 deg.C for 16 hr, centrifuging at 4000r/min for 10 min, removing supernatant, oven drying the precipitate at 50 deg.C until the water content is less than 10%, and pulverizing to 80 mesh to obtain rhizoma Gymnadeniae crude polysaccharide extract.
(b) Preparing a bletilla striata crude polysaccharide extract:
pulverizing rhizoma bletilla decoction pieces into 40 mesh fine powder, adding water, boiling and extracting for 2 times, adding 30 times of water for the first time, extracting for 3 hr, adding 20 times of water for the second time, and extracting for 1.5 hr; respectively sieving the two extracting solutions with a 350-mesh sieve, mixing the extracting solutions, and concentrating until the solid content is 3%; adding 95 vol% ethanol into the concentrated solution until the volume concentration of ethanol in the solution system is 60%, standing in a refrigerator at 4 deg.C for 16 hr, centrifuging at 4000r/min for 10 min, removing supernatant, oven drying the precipitate at 50 deg.C until the water content is less than 10%, and pulverizing to 80 mesh to obtain rhizoma bletilla crude polysaccharide extract.
(c) Preparing the astragalus crude polysaccharide extract:
cutting radix astragali decoction pieces into 5mm pieces, adding water, boiling for 2 times, extracting for 1.5 hr with 20 times of water for the first time, and extracting for 0.5 hr with 12 times of water for the second time; respectively sieving the two extracting solutions with 350 mesh sieve, mixing the extracting solutions, and concentrating until the solid content is 40%; adding 95 vol% ethanol into the concentrated solution until the volume concentration of ethanol in the solution system is 60%, standing in a refrigerator at 4 deg.C for 16 hr, centrifuging at 4000r/min for 10 min, removing supernatant, oven drying the precipitate at 50 deg.C until the water content is less than 10%, and pulverizing to 80 mesh to obtain crude polysaccharide extract of radix astragali.
(d) Taking glycerol according to the weight parts, adding the gymnadenia conopsea crude polysaccharide extract, the bletilla striata crude polysaccharide extract and the astragalus membranaceus crude polysaccharide extract, and uniformly stirring;
(e) adding trimethylglycine, baicalin, glycyrrhizin, asiaticoside and deionized water into the mixture obtained in the step (d) according to the weight parts;
(f) passing the mixture of step (e) through a 200 mesh screen;
(g) UV sterilization for 50 minutes.
Example 3
The embodiment is a skin care product with moisturizing effect and capable of improving skin comfort, and the difference between the raw material formula and the preparation method in embodiment 1 is as follows: the weight parts of the gymnadenia conopsea crude polysaccharide extract, the bletilla striata crude polysaccharide extract and the astragalus membranaceus crude polysaccharide extract are different.
The specific scheme is as follows:
the raw material formula comprises the following raw materials in parts by weight:
5 parts of gymnadenia conopsea crude polysaccharide extract, 2.5 parts of bletilla striata crude polysaccharide extract, 3 parts of astragalus membranaceus crude polysaccharide extract, 5 parts of glycerol, 0.2 part of trimethylglycine, 0.08 part of baicalin, 0.1 part of glycyrrhizin, 0.2 part of asiaticoside and 60 parts of deionized water.
The preparation method comprises the following steps:
(a) preparing a gymnadenia conopsea crude polysaccharide extract:
taking gymnadenia conopsea decoction pieces, crushing into 80-mesh fine powder, adding water for boiling-maintaining extraction for 2 times, adding 40 times of water for extraction for 2.5 hours for the first time, and adding 20 times of water for extraction for 1 hour for the second time; respectively sieving the two extracting solutions with a 350-mesh sieve, mixing the extracting solutions, and concentrating until the solid content is 4%; adding 95 vol% ethanol into the concentrated solution until the volume concentration of ethanol in the solution system is 60%, standing in a refrigerator at 4 deg.C for 16 hr, centrifuging at 4000r/min for 10 min, removing supernatant, oven drying the precipitate at 50 deg.C until the water content is less than 10%, and pulverizing to 80 mesh to obtain rhizoma Gymnadeniae crude polysaccharide extract.
(b) Preparing a bletilla striata crude polysaccharide extract:
pulverizing rhizoma bletilla decoction pieces into 40 mesh fine powder, adding water, boiling and extracting for 2 times, adding 30 times of water for the first time, extracting for 3 hr, adding 20 times of water for the second time, and extracting for 1.5 hr; respectively sieving the two extracting solutions with a 350-mesh sieve, mixing the extracting solutions, and concentrating until the solid content is 3%; adding 95 vol% ethanol into the concentrated solution until the volume concentration of ethanol in the solution system is 60%, standing in a refrigerator at 4 deg.C for 16 hr, centrifuging at 4000r/min for 10 min, removing supernatant, oven drying the precipitate at 50 deg.C until the water content is less than 10%, and pulverizing to 80 mesh to obtain rhizoma bletilla crude polysaccharide extract.
(c) Preparing the astragalus crude polysaccharide extract:
cutting radix astragali decoction pieces into 5mm pieces, adding water, boiling for 2 times, extracting for 1.5 hr with 20 times of water for the first time, and extracting for 0.5 hr with 12 times of water for the second time; respectively sieving the two extracting solutions with 350 mesh sieve, mixing the extracting solutions, and concentrating until the solid content is 40%; adding 95 vol% ethanol into the concentrated solution until the volume concentration of ethanol in the solution system is 60%, standing in a refrigerator at 4 deg.C for 16 hr, centrifuging at 4000r/min for 10 min, removing supernatant, oven drying the precipitate at 50 deg.C until the water content is less than 10%, and pulverizing to 80 mesh to obtain crude polysaccharide extract of radix astragali.
(d) Taking glycerol according to the weight parts, adding the gymnadenia conopsea crude polysaccharide extract, the bletilla striata crude polysaccharide extract and the astragalus membranaceus crude polysaccharide extract, and uniformly stirring;
(e) adding trimethylglycine, baicalin, glycyrrhizin, asiaticoside and deionized water into the mixture obtained in the step (d) according to the weight parts;
(f) passing the mixture of step (e) through a 200 mesh screen;
(g) UV sterilization for 50 minutes.
Comparative example 1
The comparative example is a skin care product with moisturizing effect and capable of improving skin comfort, and the difference between the raw material formula and the preparation method in example 1 is as follows: the rhizoma Gymnadeniae crude polysaccharide extract, rhizoma Bletillae crude polysaccharide extract and radix astragali crude polysaccharide extract are not added.
The specific scheme is as follows:
the raw material formula comprises the following raw materials in parts by weight:
5 parts of glycerin, 0.2 part of trimethyl glycine, 0.08 part of baicalin, 0.1 part of glycyrrhizin, 0.2 part of asiaticoside and 60 parts of deionized water.
The preparation method comprises the following steps:
(a) mixing glycerol, trimethylglycine, baicalin, glycyrrhizin, asiaticoside and deionized water uniformly according to the weight parts;
(b) passing the mixture of step (a) through a 200 mesh screen;
(c) UV sterilization for 50 minutes.
Comparative example 2
The comparative example is a skin care product with moisturizing effect and capable of improving skin comfort, and the difference between the raw material formula and the preparation method in example 1 is as follows: equal amount of rhizoma Gymnadeniae crude polysaccharide extract is used to replace rhizoma Bletillae crude polysaccharide extract.
The specific scheme is as follows:
the raw material formula comprises the following raw materials in parts by weight:
5.5 parts of gymnadenia conopsea crude polysaccharide extract, 5 parts of astragalus root crude polysaccharide extract, 5 parts of glycerol, 0.2 part of trimethylglycine, 0.08 part of baicalin, 0.1 part of glycyrrhizin, 0.2 part of asiaticoside and 60 parts of deionized water.
The preparation method comprises the following steps:
(a) preparing a gymnadenia conopsea crude polysaccharide extract:
taking gymnadenia conopsea decoction pieces, crushing into 80-mesh fine powder, adding water for boiling-maintaining extraction for 2 times, adding 40 times of water for extraction for 2.5 hours for the first time, and adding 20 times of water for extraction for 1 hour for the second time; respectively sieving the two extracting solutions with a 350-mesh sieve, mixing the extracting solutions, and concentrating until the solid content is 4%; adding 95 vol% ethanol into the concentrated solution until the volume concentration of ethanol in the solution system is 60%, standing in a refrigerator at 4 deg.C for 16 hr, centrifuging at 4000r/min for 10 min, removing supernatant, oven drying the precipitate at 50 deg.C until the water content is less than 10%, and pulverizing to 80 mesh to obtain rhizoma Gymnadeniae crude polysaccharide extract.
(b) Preparing the astragalus crude polysaccharide extract:
cutting radix astragali decoction pieces into 5mm pieces, adding water, boiling for 2 times, extracting for 1.5 hr with 20 times of water for the first time, and extracting for 0.5 hr with 12 times of water for the second time; respectively sieving the two extracting solutions with 350 mesh sieve, mixing the extracting solutions, and concentrating until the solid content is 40%; adding 95 vol% ethanol into the concentrated solution until the volume concentration of ethanol in the solution system is 60%, standing in a refrigerator at 4 deg.C for 16 hr, centrifuging at 4000r/min for 10 min, removing supernatant, oven drying the precipitate at 50 deg.C until the water content is less than 10%, and pulverizing to 80 mesh to obtain crude polysaccharide extract of radix astragali.
(c) Taking glycerol according to the weight parts, adding the gymnadenia conopsea crude polysaccharide extract and the astragalus root crude polysaccharide extract, and uniformly stirring;
(d) adding trimethylglycine, baicalin, glycyrrhizin, asiaticoside and deionized water into the mixture obtained in the step (c) according to the weight parts;
(e) passing the mixture from step (d) through a 200 mesh screen;
(f) UV sterilization for 50 minutes.
Comparative example 3
The comparative example is a skin care product with moisturizing effect and capable of improving skin comfort, and the difference between the raw material formula and the preparation method in example 1 is as follows: equal amount of bletilla striata crude polysaccharide extract is used for replacing the gymnadenia conopsea crude polysaccharide extract.
The specific scheme is as follows:
the raw material formula comprises the following raw materials in parts by weight:
5.5 parts of bletilla striata crude polysaccharide extract, 5 parts of astragalus membranaceus crude polysaccharide extract, 5 parts of glycerol, 0.2 part of trimethylglycine, 0.08 part of baicalin, 0.1 part of glycyrrhizin, 0.2 part of asiaticoside and 60 parts of deionized water.
The preparation method comprises the following steps:
(a) preparing a bletilla striata crude polysaccharide extract:
pulverizing rhizoma bletilla decoction pieces into 40 mesh fine powder, adding water, boiling and extracting for 2 times, adding 30 times of water for the first time, extracting for 3 hr, adding 20 times of water for the second time, and extracting for 1.5 hr; respectively sieving the two extracting solutions with a 350-mesh sieve, mixing the extracting solutions, and concentrating until the solid content is 3%; adding 95 vol% ethanol into the concentrated solution until the volume concentration of ethanol in the solution system is 60%, standing in a refrigerator at 4 deg.C for 16 hr, centrifuging at 4000r/min for 10 min, removing supernatant, oven drying the precipitate at 50 deg.C until the water content is less than 10%, and pulverizing to 80 mesh to obtain rhizoma bletilla crude polysaccharide extract.
(b) Preparing the astragalus crude polysaccharide extract:
cutting radix astragali decoction pieces into 5mm pieces, adding water, boiling for 2 times, extracting for 1.5 hr with 20 times of water for the first time, and extracting for 0.5 hr with 12 times of water for the second time; respectively sieving the two extracting solutions with 350 mesh sieve, mixing the extracting solutions, and concentrating until the solid content is 40%; adding 95 vol% ethanol into the concentrated solution until the volume concentration of ethanol in the solution system is 60%, standing in a refrigerator at 4 deg.C for 16 hr, centrifuging at 4000r/min for 10 min, removing supernatant, oven drying the precipitate at 50 deg.C until the water content is less than 10%, and pulverizing to 80 mesh to obtain crude polysaccharide extract of radix astragali.
(c) Taking the glycerol according to the weight parts, adding the bletilla striata crude polysaccharide extract and the astragalus membranaceus crude polysaccharide extract, and uniformly stirring;
(d) adding trimethylglycine, baicalin, glycyrrhizin, asiaticoside and deionized water into the mixture obtained in the step (c) according to the weight parts;
(e) passing the mixture from step (d) through a 200 mesh screen;
(f) UV sterilization for 50 minutes.
Comparative example 4
The comparative example is a skin care product with moisturizing effect and capable of improving skin comfort, and the difference between the raw material formula and the preparation method in example 1 is as follows: equal amount of bletilla striata crude polysaccharide extract is used for replacing astragalus membranaceus crude polysaccharide extract.
The specific scheme is as follows:
the raw material formula comprises the following raw materials in parts by weight:
2.5 parts of gymnadenia conopsea crude polysaccharide extract, 8 parts of bletilla striata crude polysaccharide extract, 5 parts of glycerol, 0.2 part of trimethylglycine, 0.08 part of baicalin, 0.1 part of glycyrrhizin, 0.2 part of asiaticoside and 60 parts of deionized water.
The preparation method comprises the following steps:
(a) preparing a gymnadenia conopsea crude polysaccharide extract:
taking gymnadenia conopsea decoction pieces, crushing into 80-mesh fine powder, adding water for boiling-maintaining extraction for 2 times, adding 40 times of water for extraction for 2.5 hours for the first time, and adding 20 times of water for extraction for 1 hour for the second time; respectively sieving the two extracting solutions with a 350-mesh sieve, mixing the extracting solutions, and concentrating until the solid content is 4%; adding 95 vol% ethanol into the concentrated solution until the volume concentration of ethanol in the solution system is 60%, standing in a refrigerator at 4 deg.C for 16 hr, centrifuging at 4000r/min for 10 min, removing supernatant, oven drying the precipitate at 50 deg.C until the water content is less than 10%, and pulverizing to 80 mesh to obtain rhizoma Gymnadeniae crude polysaccharide extract.
(b) Preparing a bletilla striata crude polysaccharide extract:
pulverizing rhizoma bletilla decoction pieces into 40 mesh fine powder, adding water, boiling and extracting for 2 times, adding 30 times of water for the first time, extracting for 3 hr, adding 20 times of water for the second time, and extracting for 1.5 hr; respectively sieving the two extracting solutions with a 350-mesh sieve, mixing the extracting solutions, and concentrating until the solid content is 3%; adding 95 vol% ethanol into the concentrated solution until the volume concentration of ethanol in the solution system is 60%, standing in a refrigerator at 4 deg.C for 16 hr, centrifuging at 4000r/min for 10 min, removing supernatant, oven drying the precipitate at 50 deg.C until the water content is less than 10%, and pulverizing to 80 mesh to obtain rhizoma bletilla crude polysaccharide extract.
(c) Taking glycerol according to the weight parts, adding the gymnadenia conopsea crude polysaccharide extract and the bletilla striata crude polysaccharide extract, and uniformly stirring;
(d) adding trimethylglycine, baicalin, glycyrrhizin, asiaticoside and deionized water into the mixture obtained in the step (c) according to the weight parts;
(e) passing the mixture from step (d) through a 200 mesh screen;
(f) UV sterilization for 50 minutes.
Comparative example 5
The comparative example is a skin care product with moisturizing effect and capable of improving skin comfort, and the difference between the raw material formula and the preparation method in example 1 is as follows: equal amount of pachyman extract (commercially available product, crude polysaccharide content 70.512%) was used instead of rhizoma bletilla crude polysaccharide extract.
The specific scheme is as follows:
the raw material formula comprises the following raw materials in parts by weight:
2.5 parts of gymnadenia conopsea crude polysaccharide extract, 3 parts of pachyman, 5 parts of astragalus root crude polysaccharide extract, 5 parts of glycerin, 0.2 part of trimethyl glycine, 0.08 part of baicalin, 0.1 part of glycyrrhizin, 0.2 part of asiaticoside and 60 parts of deionized water.
The preparation method comprises the following steps:
(a) preparing a gymnadenia conopsea crude polysaccharide extract:
taking gymnadenia conopsea decoction pieces, crushing into 80-mesh fine powder, adding water for boiling-maintaining extraction for 2 times, adding 40 times of water for extraction for 2.5 hours for the first time, and adding 20 times of water for extraction for 1 hour for the second time; respectively sieving the two extracting solutions with a 350-mesh sieve, mixing the extracting solutions, and concentrating until the solid content is 4%; adding 95 vol% ethanol into the concentrated solution until the volume concentration of ethanol in the solution system is 60%, standing in a refrigerator at 4 deg.C for 16 hr, centrifuging at 4000r/min for 10 min, removing supernatant, oven drying the precipitate at 50 deg.C until the water content is less than 10%, and pulverizing to 80 mesh to obtain rhizoma Gymnadeniae crude polysaccharide extract.
(b) Preparing the astragalus crude polysaccharide extract:
cutting radix astragali decoction pieces into 5mm pieces, adding water, boiling for 2 times, extracting for 1.5 hr with 20 times of water for the first time, and extracting for 0.5 hr with 12 times of water for the second time; respectively sieving the two extracting solutions with 350 mesh sieve, mixing the extracting solutions, and concentrating until the solid content is 40%; adding 95 vol% ethanol into the concentrated solution until the volume concentration of ethanol in the solution system is 60%, standing in a refrigerator at 4 deg.C for 16 hr, centrifuging at 4000r/min for 10 min, removing supernatant, oven drying the precipitate at 50 deg.C until the water content is less than 10%, and pulverizing to 80 mesh to obtain crude polysaccharide extract of radix astragali.
(c) Taking glycerol according to the weight parts, adding the crude polysaccharide extract of the gymnadenia conopsea, the pachyman and the crude polysaccharide extract of the astragalus root, and uniformly stirring;
(d) adding trimethylglycine, baicalin, glycyrrhizin, asiaticoside and deionized water into the mixture obtained in the step (c) according to the weight parts;
(e) passing the mixture from step (d) through a 200 mesh screen;
(f) UV sterilization for 50 minutes.
Example 4
The embodiment is a skin care product with moisturizing effect and capable of improving skin comfort, and the difference between the raw material formula and the preparation method in embodiment 1 is as follows: during the preparation of the gymnadenia conopsea crude polysaccharide extract, the bletilla striata crude polysaccharide extract and the astragalus membranaceus crude polysaccharide extract, ethanol with the volume concentration of 95% is added into the concentrated solution until the volume concentration of the ethanol in the solution system is 50%.
The specific scheme is as follows:
the raw material formula comprises the following raw materials in parts by weight:
2.5 parts of gymnadenia conopsea crude polysaccharide extract, 3 parts of bletilla striata crude polysaccharide extract, 5 parts of astragalus membranaceus crude polysaccharide extract, 5 parts of glycerol, 0.2 part of trimethylglycine, 0.08 part of baicalin, 0.1 part of glycyrrhizin, 0.2 part of asiaticoside and 60 parts of deionized water.
The preparation method comprises the following steps:
(a) preparing a gymnadenia conopsea crude polysaccharide extract:
taking gymnadenia conopsea decoction pieces, crushing into 80-mesh fine powder, adding water for boiling-maintaining extraction for 2 times, adding 40 times of water for extraction for 2.5 hours for the first time, and adding 20 times of water for extraction for 1 hour for the second time; respectively sieving the two extracting solutions with a 350-mesh sieve, mixing the extracting solutions, and concentrating until the solid content is 4%; adding 95 vol% ethanol into the concentrated solution until the volume concentration of ethanol in the solution system is 50%, standing in a refrigerator at 4 deg.C for 16 hr, centrifuging at 4000r/min for 10 min, removing supernatant, oven drying the precipitate at 50 deg.C until the water content is less than 10%, and pulverizing to 80 mesh to obtain rhizoma Gymnadeniae crude polysaccharide extract.
(b) Preparing a bletilla striata crude polysaccharide extract:
pulverizing rhizoma bletilla decoction pieces into 40 mesh fine powder, adding water, boiling and extracting for 2 times, adding 30 times of water for the first time, extracting for 3 hr, adding 20 times of water for the second time, and extracting for 1.5 hr; respectively sieving the two extracting solutions with a 350-mesh sieve, mixing the extracting solutions, and concentrating until the solid content is 3%; adding 95 vol% ethanol into the concentrated solution until the volume concentration of ethanol in the solution system is 50%, standing in a refrigerator at 4 deg.C for 16 hr, centrifuging at 4000r/min for 10 min, removing supernatant, oven drying the precipitate at 50 deg.C until the water content is less than 10%, and pulverizing to 80 mesh to obtain rhizoma bletilla crude polysaccharide extract.
(c) Preparing the astragalus crude polysaccharide extract:
cutting radix astragali decoction pieces into 5mm pieces, adding water, boiling for 2 times, extracting for 1.5 hr with 20 times of water for the first time, and extracting for 0.5 hr with 12 times of water for the second time; respectively sieving the two extracting solutions with 350 mesh sieve, mixing the extracting solutions, and concentrating until the solid content is 40%; concentrating 95% ethanol solution to volume concentration of 50% ethanol, standing in refrigerator at 4 deg.C for 16 hr, centrifuging at 4000r/min for 10 min, removing supernatant, oven drying the precipitate at 50 deg.C until water content is less than 10%, and pulverizing to 80 mesh to obtain radix astragali polysaccharide extract.
(d) Taking glycerol according to the weight parts, adding the gymnadenia conopsea crude polysaccharide extract, the bletilla striata crude polysaccharide extract and the astragalus membranaceus crude polysaccharide extract, and uniformly stirring;
(e) adding trimethylglycine, baicalin, glycyrrhizin, asiaticoside and deionized water into the mixture obtained in the step (d) according to the weight parts;
(f) passing the mixture of step (e) through a 200 mesh screen;
(g) UV sterilization for 50 minutes.
Example 5
The embodiment is a skin care product with moisturizing effect and capable of improving skin comfort, and the difference between the raw material formula and the preparation method in embodiment 1 is as follows: during the preparation process of the gymnadenia conopsea crude polysaccharide extract, the bletilla striata crude polysaccharide extract and the astragalus membranaceus crude polysaccharide extract, ethanol with the volume concentration of 95% is added until the volume concentration of the ethanol in a solution system is 70%.
The specific scheme is as follows:
the raw material formula comprises the following raw materials in parts by weight:
2.5 parts of gymnadenia conopsea crude polysaccharide extract, 3 parts of bletilla striata crude polysaccharide extract, 5 parts of astragalus membranaceus crude polysaccharide extract, 5 parts of glycerol, 0.2 part of trimethylglycine, 0.08 part of baicalin, 0.1 part of glycyrrhizin, 0.2 part of asiaticoside and 60 parts of deionized water.
The preparation method comprises the following steps:
(a) preparing the gymnadenia conopsea polysaccharide extract:
taking gymnadenia conopsea decoction pieces, crushing into 80-mesh fine powder, adding water for boiling-maintaining extraction for 2 times, adding 40 times of water for extraction for 2.5 hours for the first time, and adding 20 times of water for extraction for 1 hour for the second time; respectively sieving the two extracting solutions with a 350-mesh sieve, mixing the extracting solutions, and concentrating until the solid content is 4%; concentrating 95% ethanol solution to volume concentration of 70%, standing in refrigerator at 4 deg.C for 16 hr, centrifuging at 4000r/min for 10 min, removing supernatant, oven drying the precipitate at 50 deg.C until water content is less than 10%, and pulverizing to 80 mesh to obtain rhizoma Gymnadeniae crude polysaccharide extract.
(b) Preparing a bletilla striata crude polysaccharide extract:
pulverizing rhizoma bletilla decoction pieces into 40 mesh fine powder, adding water, boiling and extracting for 2 times, adding 30 times of water for the first time, extracting for 3 hr, adding 20 times of water for the second time, and extracting for 1.5 hr; respectively sieving the two extracting solutions with a 350-mesh sieve, mixing the extracting solutions, and concentrating until the solid content is 3%; adding 95 vol% ethanol into the concentrated solution until the volume concentration of ethanol in the solution system is 70%, standing in a refrigerator at 4 deg.C for 16 hr, centrifuging at 4000r/min for 10 min, removing supernatant, oven drying the precipitate at 50 deg.C until the water content is less than 10%, and pulverizing to 80 mesh to obtain rhizoma bletilla crude polysaccharide extract.
(c) Preparing the astragalus crude polysaccharide extract:
cutting radix astragali decoction pieces into 5mm pieces, adding water, boiling for 2 times, extracting for 1.5 hr with 20 times of water for the first time, and extracting for 0.5 hr with 12 times of water for the second time; respectively sieving the two extracting solutions with 350 mesh sieve, mixing the extracting solutions, and concentrating until the solid content is 40%; adding 95 vol% ethanol into the concentrated solution until the volume concentration of ethanol in the solution system is 70%, standing in a refrigerator at 4 deg.C for 16 hr, centrifuging at 4000r/min for 10 min, removing supernatant, oven drying the precipitate at 50 deg.C until the water content is less than 10%, and pulverizing to 80 mesh to obtain crude polysaccharide extract of radix astragali.
(d) Taking glycerol according to the weight parts, adding the gymnadenia conopsea polysaccharide crude extract, the bletilla striata crude polysaccharide extract and the astragalus membranaceus crude polysaccharide extract, and uniformly stirring;
(e) adding trimethylglycine, baicalin, glycyrrhizin, asiaticoside and deionized water into the mixture obtained in the step (d) according to the weight parts;
(f) passing the mixture of step (e) through a 200 mesh screen;
(g) UV sterilization for 50 minutes.
Moisturizing efficacy tests of examples 1-5 and comparative examples 1-5.
Firstly, testing the moisture retention performance
The test method comprises the following steps:
5 g/part of the skin care product prepared in each example and each comparative example is weighed in a weighing bottle and placed in a dryer filled with dry silica gel at room temperature (25 +/-1 ℃). The moisture mass (m) of the samples before standing was measured separately0) And the water mass (m) after standing for different time points (2h, 8h, 24h)n) And calculating the moisture retention rate:
moisture retention rate mn/m0*100％。
The results of the tests are shown in table 1 below:
TABLE 1
II, moisturizing efficacy evaluation test:
the test method comprises the following steps: evaluating the moisturizing effect of the skin care products and the physiological saline of each embodiment and comparative example according to QBT4256-2011 evaluation guideline for moisturizing efficacy of cosmetics; selecting 4cm × 4cm test area on healthy skin on forearm inner side, and testing skin care product at 2.0 + -0.1 mg/cm2The amount is coated for a single time, and the skin moisture content of samples in each test area before and after use is measured by a capacitance method skin moisture tester with the time interval of 1 h; 25 volunteers between the ages of 20 and 40 were selected for the test, and the same subject tested 3 areas per sample, 3 measurements of skin moisture were made per area, the measurements were averaged, and the test results were recorded for each example and comparative example. The results are shown in Table 2 below.
TABLE 2
Results of the moisturizing test and moisturizing efficacy evaluation test: as can be seen from the test results of example 1 and comparative example 1, the composition of the invention can effectively improve the moisture content of the skin and has better moisturizing effect. From the test results of the embodiment 1 and the comparative examples 2-4, the compound of the gymnadenia conopsea crude polysaccharide extract, the bletilla striata crude polysaccharide extract and the astragalus membranaceus crude polysaccharide extract has obviously better moisturizing effect than that of compound of two of the gymnadenia conopsea crude polysaccharide extract and the bletilla striata crude polysaccharide extract; example 1 compared with the test results of comparative example 5, shows the decisive effect of the polysaccharide preparation technology means of the patent on the moisture retention. Therefore, the composition provided by the invention can be used as a water replenishing and water retaining agent to effectively improve the moisture content of skin, and the compounding of the three polysaccharide extracts has the moisture-retaining synergistic effect, so that the composition is a natural safe skin care product which can effectively help the skin to replenish water and retain moisture and improve the comfort level of the skin.
The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (12)
1. The skin care composition with the moisturizing effect is characterized by comprising the following components in parts by weight: 1 to 5 parts of gymnadenia conopsea crude polysaccharide extract, 1 to 5 parts of bletilla striata crude polysaccharide extract and 2 to 8 parts of astragalus membranaceus crude polysaccharide extract.
2. The skin care composition with moisturizing effect according to claim 1, which is characterized by comprising the following components in parts by weight: 2 to 3 parts of gymnadenia conopsea crude polysaccharide extract, 2 to 3 parts of bletilla striata polysaccharide crude extract and 5 to 6 parts of astragalus membranaceus crude polysaccharide extract.
3. The skin care composition with moisturizing effect according to claim 2, which is characterized by comprising the following components in parts by weight: 2.3 to 2.7 parts of gymnadenia conopsea crude polysaccharide extract, 2.5 to 3 parts of bletilla striata crude polysaccharide extract and 5 to 5.5 parts of astragalus membranaceus crude polysaccharide extract.
4. The skin care composition with moisturizing effect as claimed in any one of claims 1 to 3, wherein the preparation method of the gymnadenia conopsea crude polysaccharide extract comprises the following steps:
extracting rhizoma Gymnadeniae decoction pieces with water, concentrating the extractive solution, and preparing into concentrated solution;
adding ethanol into the concentrated solution to ensure that the volume concentration of the ethanol in the solution system is 50-80%, standing the obtained solution system at the temperature of 1-10 ℃, and collecting solids.
5. The skin care composition with moisturizing effect as claimed in claim 4, wherein the water extraction is performed twice by adding water to preserve boiling, the water added for the first time is 25-40 times of the weight of the gymnadenia conopsea decoction pieces and extracted for 1.5-3 hours, and the water added for the second time is 15-30 times of the weight of the gymnadenia conopsea decoction pieces and extracted for 1-1.5 hours.
6. The skin care composition with moisturizing effect as claimed in any one of claims 1 to 3, wherein the preparation method of the bletilla striata crude polysaccharide extract comprises the following steps:
extracting rhizoma bletilla decoction pieces with water, concentrating the extractive solution, and making into concentrated solution;
adding ethanol into the concentrated solution to ensure that the volume concentration of the ethanol in the solution system is 50-80%, standing the obtained solution system at the temperature of 1-10 ℃, and collecting solids.
7. The skin care composition with moisturizing effect as claimed in claim 6, wherein the water extraction is performed twice by adding water to preserve boiling, the water added in the amount 20-35 times of the weight of the bletilla striata decoction pieces is added for the first time for 1.5-3 hours, and the water added in the amount 15-30 times of the weight of the bletilla striata decoction pieces is added for the second time for 1-1.5 hours.
8. The skin care composition with moisturizing effect as claimed in any one of claims 1 to 3, wherein the preparation method of the crude astragalus polysaccharide extract comprises the following steps:
extracting radix astragali decoction pieces with water, concentrating the obtained extractive solution, and preparing into concentrated solution;
adding ethanol into the concentrated solution to ensure that the volume concentration of the ethanol in the solution system is 50-80%, standing the obtained solution system at the temperature of 1-10 ℃, and collecting solids.
9. The skin care composition with moisturizing effect as claimed in claim 8, wherein the water extraction is performed twice by adding water to preserve boiling, the water added in an amount 10-20 times the weight of the astragalus decoction pieces is added for the first time for 1-2 hours, and the water added in an amount 10-15 times the weight of the bletilla striata decoction pieces is added for the second time for 0.5-1 hour.
10. A skin care product, which is characterized by comprising the skin care composition with moisturizing effect of any one of claims 1 to 9 and a skin care substrate.
11. The skin care product of claim 10, wherein the skin care base comprises at least one of glycerin, trimethylglycine, baicalin, glycyrrhizin, and asiaticoside.
12. The skin care product of claim 11, comprising the following components in parts by weight:
1 to 5 parts of gymnadenia conopsea crude polysaccharide extract, 1 to 5 parts of bletilla striata crude polysaccharide extract, 2 to 8 parts of astragalus membranaceus crude polysaccharide extract, 0.1 to 10 parts of glycerol, 0.1 to 5 parts of trimethylglycine, 0.01 to 3 parts of baicalin, 0.01 to 5 parts of glycyrrhizin, 0.01 to 5 parts of asiaticoside and 0 to 70 parts of water.
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|---|---|---|---|---|
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103120759A (en) * | 2012-12-28 | 2013-05-29 | 黄云湖 | Supercritical extraction method of conic gymnadenia tuber element |
| CN104127331A (en) * | 2014-08-07 | 2014-11-05 | 广东一方制药有限公司 | Skin-protecting product for improving skin anti-allergy and regeneration capacities and preparation method thereof |
| CN106309275A (en) * | 2016-08-29 | 2017-01-11 | 薛常莲 | Skin-moisturizing acne-removing lotion and preparation method thereof |
| CN107184507A (en) * | 2017-05-09 | 2017-09-22 | 南京扬子茉莉谷文化科技有限公司 | A kind of snowball herbal skin care product |
| CN109939057A (en) * | 2019-04-08 | 2019-06-28 | 上海浩泰生物科技有限公司 | Rhizoma gymnadeniae extracting method and related gymnadenia conopsea extraction |
| CN110236981A (en) * | 2019-07-09 | 2019-09-17 | 华南理工大学 | A kind of whitening plant polyose composition and its application |
| CN110693758A (en) * | 2019-10-22 | 2020-01-17 | 吉林化工学院 | Preparation and quality evaluation of compound bletilla striata facial mask |
| CN112274462A (en) * | 2020-09-17 | 2021-01-29 | 浙江森马服饰股份有限公司 | Composite bacteriostatic agent with bacteriostatic and epidermal moisturizing effects and preparation and application thereof |
-
2021
- 2021-05-17 CN CN202110534602.5A patent/CN113208944B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103120759A (en) * | 2012-12-28 | 2013-05-29 | 黄云湖 | Supercritical extraction method of conic gymnadenia tuber element |
| CN104127331A (en) * | 2014-08-07 | 2014-11-05 | 广东一方制药有限公司 | Skin-protecting product for improving skin anti-allergy and regeneration capacities and preparation method thereof |
| CN106309275A (en) * | 2016-08-29 | 2017-01-11 | 薛常莲 | Skin-moisturizing acne-removing lotion and preparation method thereof |
| CN107184507A (en) * | 2017-05-09 | 2017-09-22 | 南京扬子茉莉谷文化科技有限公司 | A kind of snowball herbal skin care product |
| CN109939057A (en) * | 2019-04-08 | 2019-06-28 | 上海浩泰生物科技有限公司 | Rhizoma gymnadeniae extracting method and related gymnadenia conopsea extraction |
| CN110236981A (en) * | 2019-07-09 | 2019-09-17 | 华南理工大学 | A kind of whitening plant polyose composition and its application |
| CN110693758A (en) * | 2019-10-22 | 2020-01-17 | 吉林化工学院 | Preparation and quality evaluation of compound bletilla striata facial mask |
| CN112274462A (en) * | 2020-09-17 | 2021-01-29 | 浙江森马服饰股份有限公司 | Composite bacteriostatic agent with bacteriostatic and epidermal moisturizing effects and preparation and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| 孙志涛等: "羧甲基化黄芪多糖的制备及其保润性能", 《天然产物研究与开发》 * |
| 黄瑞豪: "化妆品中保湿功效成分的前沿进展", 《当代化工研究》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116178586A (en) * | 2023-03-30 | 2023-05-30 | 金创克(珠海)生物技术有限公司 | Application of bletilla striata polysaccharide in preparation of product with moisturizing effect |
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| CN113208944B (en) | 2022-09-06 |
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Effective date of registration: 20231117 Address after: No. 21 and 22, Qifeng Industrial Zone, Ganjiao Community, Lishui Town, Nanhai District, Foshan City, Guangdong Province, 528200 Patentee after: Guangdong Qifeng Health Industry Co.,Ltd. Address before: 528244 Qifeng Industrial Development Zone, Lishui Town, Nanhai District, Foshan City, Guangdong Province Patentee before: GUANGDONG YIFANG PHARMACEUTICAL Co.,Ltd. |