CN115068398B

CN115068398B - Saffron crocus composition with anti-aging and hair care effects and application thereof

Info

Publication number: CN115068398B
Application number: CN202210869235.9A
Authority: CN
Inventors: 和寿祥; 和寿英; 和杰
Original assignee: Diqing Shangri La Jinke Biological Resource Development Co ltd
Current assignee: Diqing Shangri La Jinke Biological Resource Development Co ltd
Priority date: 2022-07-21
Filing date: 2022-07-21
Publication date: 2023-03-17
Anticipated expiration: 2042-07-21
Also published as: CN115068398A

Abstract

The invention provides a saffron crocus composition with anti-aging and hair-care effects and application thereof. The saffron crocus composition with the effects of resisting aging and caring hair comprises the following components in parts by weight: 8-10 parts of saffron extract, 3-5 parts of dark plum extract and 1-2 parts of resveratrol; wherein the stigma croci Sativi extract is obtained by performing enzymolysis and extraction on stigma croci Sativi, the enzyme used for enzymolysis is mixed enzyme containing protease and cellulase, the extraction solution contains ethanol, and the concentration of ethanol in the extraction solution is 60-80wt%. The saffron crocus composition has high content of active ingredients, has obvious synergistic effect among the components, can effectively remove free radicals, delay skin aging and repair hair scales, and has multiple excellent effects of resisting aging, protecting hair and the like.

Description

Saffron crocus composition with anti-aging and hair care effects and application thereof

Technical Field

The invention relates to the technical field of traditional Chinese medicine extraction, in particular to a saffron crocus composition with anti-aging and hair-care effects and application thereof.

Background

Various biomolecules in organisms have a large number of reactive groups that interact chemically to slowly crosslink the biomolecules toward chemical stability. With the lapse of time, the crosslinking degree is increased continuously, the active groups of the biomolecules are consumed and reduced continuously, the original molecular structure is changed gradually, and the aging phenomenon of the biological tissues can be caused by the accumulation of the changes. The aging causes mainly include excessive oxidation, cell life, protein aging, endocrine hypofunction, microcirculation disturbance, etc., and oxygen free radicals are important factors for accelerating aging of skin and body.

The antiaging Chinese medicinal materials comprise Polygoni Multiflori radix, radix astragali, ginseng radix, cordyceps, notoginseng radix, ganoderma, fructus Lycii, etc. Modern research finds that: the polygonum multiflorum can promote the growth of nerve cells, regulate serum cholesterol and reduce blood sugar, and has good antioxidation; the astragalus can expand coronary artery, improve myocardial blood supply, improve immunologic function and delay the process of cell aging; ginseng radix has effects of resisting oxidation, resisting aging, relieving fatigue, protecting liver, regulating cardiovascular function, and exciting hemopoietic system; cordycepin has effects of restoring metabolism of human body, regulating balance of human body, and resisting aging; the notoginseng can expand blood vessels, reduce vascular resistance, increase cardiac output, slow down heart rate, reduce myocardial oxygen consumption and capillary permeability; the ganoderma lucidum has the functions of regulating immunity, removing free radicals, balancing metabolism and the like, and can directly influence the aging process of a human body; fructus Lycii has effects of resisting arteriosclerosis, reducing blood sugar, promoting liver cell regeneration, etc., and has effects of improving body condition and delaying aging. Aiming at the problems of effective efficacy of a single traditional Chinese medicine raw material and the like, the prior art also provides a composition of a plurality of traditional Chinese medicine raw materials, however, the compositions are usually simple in efficacy superposition, and the components do not have obvious synergistic effect, so that the efficacy of each traditional Chinese medicine raw material is not favorably exerted to the maximum extent.

In view of this, the invention is particularly proposed.

Disclosure of Invention

The invention aims to provide a saffron composition with anti-aging and hair-care effects and application thereof.

The invention provides a saffron composition with anti-aging and hair care effects, which comprises the following components in parts by weight: 8-10 parts of saffron extract, 3-5 parts of dark plum extract and 1-2 parts of resveratrol.

The inventor discovers that the saffron extract, the dark plum extract and the resveratrol can form an obvious synergistic effect by screening a large number of traditional Chinese medicine raw materials and active components, so that the effects of various active ingredients in the saffron extract and the dark plum extract can be fully exerted, the utilization rate of raw materials is improved, and the saffron composition consisting of the saffron extract, the dark plum extract and the resveratrol has multiple excellent effects of resisting aging, protecting hair and the like.

Stigma croci Sativi is the upper part and stigma of stigma croci Sativi of Iridaceae; the main functional indications are activating blood circulation to dissipate blood stasis, and dispersing stagnation and resolving masses; for melancholy, chest distress, hematemesis, typhoid fever, mania, panting, amenorrhea, postpartum abdominal pain due to blood stasis, and traumatic injury. The research shows that: the chemical components of saffron mainly include flavonoid, volatile oil, carotenoid, crocetin, etc., especially the 6,8-diol compound in saffron has excellent anti-inflammatory and anti-senility activity.

Although saffron contains the active ingredients, how to fully extract various active ingredients in saffron and exert the respective efficacies to the maximum extent is still a problem in the development and utilization of saffron. The invention prepares the saffron extract by carrying out enzymolysis and extraction on saffron, wherein the enzyme adopted by the enzymolysis is mixed enzyme containing protease and cellulase, the extraction solution adopted by the extraction contains ethanol, and the concentration of the ethanol in the extraction solution is 60-80wt%.

The research finds that: firstly, the saffron is subjected to enzymolysis by mixed enzyme, protease is utilized to remove the interference of protein impurities in the raw material extraction process, and simultaneously, the insoluble cellulose in the raw material is decomposed by cellulase, so that the active ingredients in the traditional Chinese medicine raw materials are released to the maximum extent, and the subsequent extraction and purification processes are facilitated.

The enzymolysis conditions are not strictly limited and can be reasonably set according to actual needs. Specifically, the mass ratio of the protease to the cellulase in the mixed enzyme may be (1-2): (0.5-0.8); in addition, the mass ratio of saffron to mixed enzyme can be controlled to be 1000: (1.5-2.8), the enzymolysis temperature can be 45-55 ℃, and the enzymolysis time can be 3-4h. The protease and cellulase to be used are not particularly limited, and for example, neutral protease and neutral cellulase can be used.

Further, the extraction solution used for extraction can also contain ammonium sulfate, and the concentration of the ammonium sulfate in the extraction solution can be 10-15wt%; in this case, the extraction is performed using an extraction solution containing ethanol and ammonium sulfate, and the solvent used for the extraction solution may be water. The research finds that: the mixed solution containing ethanol and ammonium sulfate is used for extraction, so that useful active ingredients in the raw materials can be effectively extracted, impurity ingredients which are unfavorable for the effects of resisting aging, caring hair and the like are eliminated, and the prepared saffron extract has better effect.

The extraction conditions are not strictly limited and can be reasonably set according to actual needs. Specifically, the mass ratio of saffron to the extraction solution can be controlled to be 1: (8-15), the extraction temperature is 60-70 ℃, and the extraction time is 1-2h.

Further, after the extracting, the method can also comprise the following steps: adsorbing the extract by macroporous resin, and eluting by eluent after adsorption; wherein, the macroporous resin can adopt HPD826 type macroporous resin; the eluent can adopt 60-80wt% ethanol solution; in addition, the loading volume during adsorption may be 0.8 to 1.2 column volumes, and the volume of the eluent during elution may be 4 to 5 column volumes. The purification process can concentrate active ingredients and remove other impurities, and the effect of the purified stigma croci Sativi extract is further improved.

The smoked plum is a dried nearly mature fruit of a rosaceous plant plum, and has the effects of astringing lung, astringing intestine, promoting fluid production and calming roundworm; it is commonly indicated for chronic cough due to lung deficiency, chronic diarrhea and dysentery, diabetes due to deficiency heat, vomiting and abdominal pain due to ascariasis. The research shows that: the main chemical components of the dark plum comprise organic acid, flavone, saccharide, terpenes, sterols, lipid, alkaloid, volatile components and the like, and the pharmacological effects of the dark plum comprise bacteriostasis, antianaphylaxis, antioxidation and the like.

In the invention, the dark plum extract is prepared by adding water into dark plum and heating, refluxing and extracting; the heating reflux extraction conditions are not strictly limited and can be reasonably set according to actual needs. Specifically, the mass ratio of the dark plum fruit to the water can be controlled to be 1: (10-15), the reflux extraction temperature is 60-80 ℃, and the reflux extraction time is 2-4h.

Furthermore, the saffron composition also comprises 0.1 to 0.3 portion of hydroxyl tetrahydropyrimidine and/or 0.2 to 0.4 portion of vitamin C palmitate. The research finds that the addition of the components can further enhance various efficacies of the saffron composition.

The invention also provides application of the saffron composition in preparation of skin care products or hair care products.

Specifically, the content of the saffron composition in the skin care product or the hair care product can be 1-5%.

The implementation of the invention has at least the following advantages:

1. in the saffron composition, the three components of the saffron extract, the dark plum extract and the resveratrol can form an obvious synergistic effect, so that the effects of various active ingredients in the saffron extract and the dark plum extract can be fully exerted, and the utilization rate of raw materials and the effect of products are improved;

2. the invention prepares the saffron extract by carrying out enzymolysis and extraction on the saffron, can fully extract various active ingredients in the saffron, thereby exerting the efficacy of each active ingredient to the maximum extent, and the prepared saffron extract has high effective active ingredients, low impurity content and better efficacy;

3. the saffron composition also comprises hydroxyl tetrahydropyrimidine and/or vitamin C palmitate, the content of active ingredients in the composition is high, the components have obvious synergistic action, free radicals can be effectively eliminated, skin aging is delayed, hair scales are repaired, and the saffron composition has multiple excellent effects of resisting aging, protecting hair and the like.

Detailed Description

It should be noted that the following detailed description is exemplary and is intended to provide further explanation of the disclosure. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.

It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments according to the present application. As used herein, the singular forms also include the plural forms unless the context clearly dictates otherwise, and further, it is understood that when the terms "comprises" and/or "comprising" are used in this specification, they specify the presence of the stated features, steps, operations, devices, components, and/or combinations thereof.

The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments, and it should be understood that the described embodiments are some, but not all, embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example 1

1. Preparation of saffron extract

1) Cleaning stigma croci Sativi, drying, grinding to 100 mesh to obtain stigma croci Sativi powder.

2) Mixing saffron crocus powder and water according to a mass ratio of 1:8, mixing, and adding neutral protease and neutral cellulase for enzymolysis, wherein the mass ratio of the neutral protease to the neutral cellulase is 1:0.5, the mass ratio of the saffron crocus powder to the total mass of the neutral protease and the neutral cellulase is 1000:1.5, the enzymolysis temperature is 55 ℃, and the enzymolysis time is 3 hours, so as to obtain the enzymolysis liquid.

3) Adding ethanol into the enzymolysis solution, controlling the final concentration of the ethanol to be 80wt%, and extracting at 80 deg.C for 1h to obtain stigma croci Sativi extract.

4) Filtering, concentrating, and drying the extractive solution to obtain stigma croci Sativi extract.

2. Preparation of a dark plum extract

1) Removing cores of the dark plums, crushing dark plum pulp, and sieving with a 40-mesh sieve to obtain dark plum coarse powder.

2) Mixing the dark plum coarse powder and water according to a mass ratio of 1:10 mixing, and heating and refluxing at 80 deg.C for 2 hr to obtain fructus mume extractive solution.

3) Filtering, concentrating and drying the dark plum extract to obtain the dark plum extract.

3. Preparation of saffron composition

And uniformly mixing 8 parts of saffron extract, 5 parts of dark plum extract and 2 parts of resveratrol to obtain the saffron composition.

Example 2

1. Preparation of saffron extract

1) Cleaning stigma croci Sativi, drying, and grinding to 80 mesh to obtain stigma croci Sativi powder.

2) Mixing saffron crocus powder and water according to a mass ratio of 1:15, adding neutral protease and neutral cellulase for enzymolysis, wherein the mass ratio of the neutral protease to the neutral cellulase is 2:0.8, the mass ratio of the saffron crocus powder to the total mass of the neutral protease and the neutral cellulase is 1000:2.8, the enzymolysis temperature is 45 ℃, and the enzymolysis time is 4 hours, so as to obtain the enzymolysis liquid.

3) Adding ethanol into the enzymolysis solution, controlling the final concentration of ethanol to be 60wt%, and extracting at 60 deg.C for 2h to obtain stigma croci Sativi extract.

2. Preparation of a dark plum extract

1) Removing cores of the dark plums, crushing dark plum pulp, and sieving with a 30-mesh sieve to obtain dark plum coarse powder.

2) Mixing the dark plum coarse powder and water according to a mass ratio of 1:15, heating and refluxing for extraction at the reflux extraction temperature of 60 ℃ for 4 hours to obtain the dark plum extract.

3. Preparation of saffron composition

Mixing 10 parts of saffron extract, 3 parts of dark plum extract and 1 part of resveratrol uniformly to obtain the saffron composition.

Example 3

The same procedure as in example 1 was repeated except that the procedure for preparing saffron extract was different.

This example prepares saffron extract as follows:

1) Cleaning stigma croci Sativi, drying, and grinding to 100 mesh to obtain stigma croci Sativi powder.

2) Mixing saffron crocus powder and water according to a mass ratio of 1:10, adding neutral protease and neutral cellulase for enzymolysis, wherein the mass ratio of the neutral protease to the neutral cellulase is 1.5:0.5, the mass ratio of the saffron crocus powder to the total mass of the neutral protease and the neutral cellulase is 1000:2, the enzymolysis temperature is 50 ℃, and the enzymolysis time is 3.5 hours, so as to obtain the enzymolysis liquid.

3) Adding ethanol and ammonium sulfate into the enzymolysis solution, controlling the final concentration of ethanol to be 80wt% and the final concentration of ammonium sulfate to be 10wt%, and extracting at 70 deg.C for 1.5h to obtain stigma croci Sativi extract.

4) Filtering and concentrating the saffron extract, and then adsorbing the concentrated solution by adopting HPD826 type macroporous resin, wherein the sample loading volume is 1 time of the column volume; after the sample loading is finished, 80wt% ethanol solution is adopted for elution, the elution volume is 4 times of the column volume, and the eluent is collected.

5) Filtering, concentrating, and drying the eluate to obtain stigma croci Sativi extract.

Example 4

The same procedure as in example 3 was followed except that the procedure for preparing the saffron composition was different.

The procedure for the preparation of saffron composition of this example was: mixing stigma croci Sativi extract 8 parts, mume fructus extract 5 parts, resveratrol 2 parts and hydroxy tetrahydropyrimidine 0.3 parts to obtain stigma croci Sativi composition.

Example 5

The procedure for the preparation of saffron composition of this example was: mixing stigma croci Sativi extract 8 parts, mume fructus extract 5 parts, resveratrol 2 parts and vitamin C palmitate 0.3 parts to obtain stigma croci Sativi composition.

Example 6

The steps for preparing the saffron composition of this example are: mixing stigma croci Sativi extract 8 parts, mume fructus extract 5 parts, resveratrol 2 parts, hydroxy tetrahydropyrimidine 0.1 part and vitamin C palmitate 0.2 part to obtain stigma croci Sativi composition.

Comparative example 1

The comparative example is basically the same as the example 1 except that the step of preparing the saffron extract is not subjected to enzymolysis; specifically, the steps of preparing the safflower extract of this comparative example were:

2) Mixing saffron crocus powder and water according to a mass ratio of 1:8 mixing, adding ethanol to a final concentration of 80wt%, and extracting at 80 deg.C for 1 hr to obtain stigma croci Sativi extractive solution.

3) Filtering, concentrating, and drying the extractive solution to obtain stigma croci Sativi extract.

Comparative example 2

The procedure of example 1 was repeated except that resveratrol was not added in the step of preparing the saffron composition.

Comparative example 3

The same procedure as in example 1 was repeated except that no ume extract was added in the step of preparing the saffron composition.

Comparative example 4

The same procedure as in example 1 was repeated except that no saffron extract was added in the step of preparing the saffron composition.

Comparative example 5

Substantially the same as example 1 except that the polygonum multiflorum extract is used in place of the prunus mume extract of example 1; the procedure for preparing the polygonum multiflorum extract in this comparative example was:

1) Pulverizing Polygoni Multiflori radix, and sieving with 40 mesh sieve to obtain Polygoni Multiflori radix coarse powder.

2) Mixing the polygonum multiflorum coarse powder and water according to a mass ratio of 1:10, heating and refluxing for 2 hours at the reflux extraction temperature of 80 ℃ to obtain the polygonum multiflorum extracting solution.

3) Filtering, concentrating and drying the polygonum multiflorum extracting solution to obtain the polygonum multiflorum extract.

Comparative example 6

The procedure of example 1 was repeated except that commercially available dendrobium officinale extract was used in place of the prunus mume extract of example 1.

Comparative example 7

The procedure of example 1 was repeated except that acetylated hexapeptide was used in place of resveratrol in the step of preparing saffron composition.

Comparative example 8

The steps for preparing the saffron composition of the comparative example are as follows: mixing 8 parts of saffron extract, 5 parts of dark plum extract, 2 parts of sodium hyaluronate, 0.1 part of ursolic acid and 0.2 part of vitamin C uniformly to obtain the saffron composition.

Test example 1 anti-aging Property measurement

The compositions of each example and the comparative example are respectively added into certain commercially available moisturizing milk in an amount of 5%, and are stirred and mixed uniformly to prepare the corresponding anti-aging milk (i.e. a sample to be tested).

1. DPPH radical scavenging ability test

Adding 3mL of sample to be detected into 10mL of colorimetric tube, adding 3.0mL of 0.15mM DPPH methanol solution, shaking, reacting at room temperature in a dark place for 30min, and measuring the absorbance A at the wavelength of 517nm ₁ (ii) a Under the same condition, methanol is adopted to replace DPPH solution to measure the background absorbance value A ₂ While replacing the sample with the sample solvent, and determining the blank value A ₀ 。

The DPPH clearance was calculated as follows:

DPPH clearance (%) = [ A ] ₀ -(A ₁ -A ₂ )]/A ₀ ×100％。

2. Skin elasticity test

Randomly selecting 10 female volunteers from each group, wherein the age of the volunteers is 30-40 years, each volunteer takes the anti-aging milk of each embodiment and the control example every day and coats the anti-aging milk on the cheek, and the anti-aging milk is used twice a day, once in the morning and evening, 1mL each time and continuously used for 30 days; meanwhile, moisturizing milk without the added composition is used as a blank group.

The skin firmness of each volunteer before and after the anti-aging milk is used is detected by adopting a skin elasticity tester (Corneometer MPA 580), the skin firmness detection data is recorded, and the change rate of the skin firmness is calculated according to the following formula.

The rate of change in skin firmness (%) = [ (skin firmness 30 days after anti-aging application-skin firmness before anti-aging application)/skin firmness before anti-aging application ] × 100%.

3. Skin wrinkle testing

The skin wrinkles of each volunteer before and 30 days after the anti-aging milk is used are detected by adopting a skin rapid three-dimensional imaging system PRIMOS, the skin wrinkle detection data are recorded, and the change rate of the skin wrinkles is calculated according to the following formula.

Change rate of skin wrinkles (%) = [ (skin wrinkles after 30 days using moisturizing cream-skin wrinkles before using moisturizing cream)/skin wrinkles before using moisturizing cream ] × 100%.

The results are shown in Table 1.

Test example 2 Hair Condition Performance test

The compositions of the embodiments and the comparative examples are respectively added into a certain hair conditioner which is purchased in the market according to the addition amount of 5 percent, and the mixture is stirred and mixed evenly, thus obtaining the hair conditioner to be tested.

The repair effect verification test method comprises the following steps:

1) Taking 10 cm-long hair, and grouping every 10 hair. Each group of hair was washed with 2g of a 3% by mass sodium lauryl sulfate solution and mechanically kneaded for 5 minutes, and then the state of destruction of the cell structure of the hair scale was observed under a microscope and counted and labeled.

2) Soaking the hair in the hair conditioner to be tested in each example and the hair conditioner to be tested in the comparative example respectively, stirring continuously, taking out after 30min, washing with clear water for 3 times, each time for 1 min, and then naturally drying.

3) Observing each group of dried hair under a microscope, counting the repair condition of the damaged hair scale cell structure on the surface of the hair marked in the step 1) and calculating the repair rate of the hair scales according to the following formula:

the hair scale repair rate (%) = total number of repaired hair scale cells/total number of damaged hair scale cells × 100%.

The results of repairing the cellular structure of damaged hair scales are shown in Table 1.

TABLE 1 anti-aging and Hair-protecting Properties test results

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. The saffron crocus composition with the effects of resisting aging and protecting hair is characterized by comprising the following components in parts by weight: 8-10 parts of saffron extract, 3-5 parts of dark plum extract and 1-2 parts of resveratrol; wherein, the saffron extract is obtained by carrying out enzymolysis and extraction on saffron, the enzyme adopted by the enzymolysis is a mixed enzyme containing protease and cellulase, and the mass ratio of the protease to the cellulase in the mixed enzyme is (1-2): (0.5-0.8), controlling the mass ratio of saffron to mixed enzyme to be 1000: (1.5-2.8), the enzymolysis temperature is 45-55 ℃, the enzymolysis time is 3-4h, the extraction solution adopted for extraction contains ethanol, the concentration of the ethanol in the extraction solution is 60-80wt%, and the mass ratio of the saffron crocus to the extraction solution is controlled to be 1: (8-15), extracting at the temperature of 60-80 ℃ for 1-2h; the dark plum extract is prepared by adding water into dark plum and heating and refluxing for extraction, wherein the mass ratio of dark plum to water is controlled to be 1: (10-15), the reflux extraction temperature is 60-80 ℃, and the reflux extraction time is 2-4h.

2. The saffron composition according to claim 1, further comprising 0.1 to 0.3 parts of hydroxytetrahydropyrimidine and/or 0.2 to 0.4 parts of vitamin C palmitate.

3. The saffron composition according to claim 1, wherein the extraction solution used for extraction further comprises ammonium sulfate, the concentration of ammonium sulfate in the extraction solution is 10-15wt%.

4. Saffron composition according to claim 1, further comprising after extraction: adsorbing the extract by macroporous resin, and eluting by eluent after adsorption.

5. Saffron composition according to claim 4, wherein the macroporous resin is HPD826 type macroporous resin.

6. Saffron composition according to claim 4, wherein the elution is an ethanol solution with a concentration of 60-80wt%.

7. Use of saffron composition according to any one of claims 1 to 6 in the preparation of a skin or hair care product.

8. Use according to claim 7, characterized in that the saffron composition is present in a skin or hair care product in an amount of 1-5%.