CN109331074B - Traditional Chinese medicine composition with anti-aging effect and preparation method and application thereof - Google Patents
Traditional Chinese medicine composition with anti-aging effect and preparation method and application thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/481—Astragalus (milkvetch)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/63—Oleaceae (Olive family), e.g. jasmine, lilac or ash tree
- A61K36/638—Ligustrum, e.g. Chinese privet
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/65—Paeoniaceae (Peony family), e.g. Chinese peony
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/74—Rubiaceae (Madder family)
- A61K36/744—Gardenia
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/18—Antioxidants, e.g. antiradicals
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
- A61P39/06—Free radical scavengers or antioxidants
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
Abstract
The invention relates to a traditional Chinese medicine composition with an anti-aging effect, and a preparation method and application thereof, wherein the traditional Chinese medicine composition is prepared from the following raw materials in percentage by mass: 20-50% of peony, 10-30% of gardenia, 10-30% of astragalus membranaceus root and 10-30% of glossy privet seed. The traditional Chinese medicine composition contains multiple anti-aging ingredients, the four traditional Chinese medicines are proportioned according to the optimal proportion, the component formula is reasonable, the active ingredients supplement each other, the synergy can be realized, the prepared cosmetics are mild in property, and the quick and long-acting anti-aging effect can be generated.
Description
Technical Field
The invention belongs to the field of traditional Chinese medicines, relates to a traditional Chinese medicine composition, a preparation method and application thereof, and particularly relates to a traditional Chinese medicine composition with an anti-aging effect, a preparation method and application thereof.
Background
With the increase of age, the metabolism frequency of epidermal cells of a human body is reduced, and the epidermal barrier capacity is gradually weakened; in addition, the skin is easy to have skin phenomena such as primary lines, fine lines, wrinkles, age pigment and the like due to the stimulation of external environment pollution and the irradiation of ultraviolet rays.
The aging mechanism is still in the research stage, and there are many theories such as the theory of somatic mutation, the theory of free radicals, the theory of neuroendocrine regulation, and the like. The organism oxidation reaction is more and more intense in the aging process, such as the generation of free radicals capable of causing DNA damage expression, the organism gradually loses the scavenging capacity of the free radicals due to the aging of cells, and the reaction force of skin to the stimulating growth factors is weakened and repeated due to excessive free radicals, so that the aging of the organism is accelerated; the skin aging-delaying cosmetic has the advantages of short aging, quick response and the like by improving the oxidation resistance of the skin, but the oxidation resistance can not be ensured for a long time along with the periodic metabolism of epidermis. The traditional Chinese medicine theory considers that human aging is gradually changed from inside to outside, mainly caused by deficiency of qi and blood, qi stagnation and blood stasis of the human body, and deficiency of liver-yin and kidney-yin, and the traditional Chinese medicine theory adjusts and nourishes the interior and the exterior to delay the aging of organisms for a long time, but has the defect of long time consumption. The technical difficulty in the field is how to rapidly remove free radicals of the skin, enhance the self oxidation resistance of the skin, reduce the generation of fine lines and achieve the aim of delaying skin aging.
CN107007501A discloses an anti-aging composition comprising ginseng extract, equisetum extract and arillus longan extract. The three components complement each other in action mechanism, and the combined use has more obvious anti-aging effect. The preparation method of the anti-aging composition provided by the invention is simple, the production time is saved, the stability of active ingredients can be kept in the preparation process, and the product also has an obvious free radical scavenging effect.
CN108451811A discloses an anti-aging traditional Chinese medicine facial mask, which contains composite anti-aging ingredients: 10-15 parts of polygonum multiflorum, 15-20 parts of medlar, 12-18 parts of ginkgo leaves, 8-12 parts of angelica, 9-15 parts of mulberries, 6-10 parts of tea leaves and 11-16 parts of carrots; the composite anti-aging component has the advantages of synergy, good permeability, easy absorption and the like, can quickly permeate and be absorbed by skin cells, maintains the normal metabolism of the skin cells, enables the skin cells to be in a healthy state for a long time, enhances the anti-aging capability of the skin cells, and is not easy to relax or cause diseases; the facial mask has effects of improving skin resistance to achieve antiaging effect, and has advantages of mild medicinal effect, good antiaging effect, and no adverse side effect.
CN105030610A discloses an anti-aging composition, comprising a base component and anti-aging factors dispersed in the base component, wherein the anti-aging factors are selected from at least three of soybean germ extract, wheat germ extract, safflower germ extract and corn germ extract. At least three of the soybean germ extract, the wheat germ extract, the safflower germ extract and the corn germ extract generate a synergistic anti-aging effect mutually, and can synergistically promote the synthesis of collagen, quickly smooth fine lines of skin and repair damaged skin, thereby achieving the purpose of delaying skin aging.
However, the above prior art is difficult to achieve the effect of rapidly and long-acting improving the anti-aging ability of the skin, so it is very necessary to develop a drug capable of rapidly and long-acting relieving aging.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide a traditional Chinese medicine composition and a preparation method and application thereof, and particularly provides a traditional Chinese medicine composition with an anti-aging effect and a preparation method and application thereof.
In order to achieve the purpose, the invention adopts the following technical scheme:
in a first aspect, the invention provides a traditional Chinese medicine composition, and the raw materials for preparing the traditional Chinese medicine composition comprise the following components in percentage by mass: 20-50% of peony, 10-30% of gardenia, 10-30% of astragalus membranaceus root and 10-30% of glossy privet seed.
Wherein peony contains abundant flavonoids, anthocyan, polyphenols, proteins, and amino acids, has strong oxidation resistance, and can directly remove O2-、H2O2The active oxygen is one of the active compounds with stronger antioxidant capacity discovered at present, and has obvious anti-aging effect.
The gardenia mainly contains iridoid, flavonoid, diterpenoid, phenolic acid, organic acid ester and other compounds, and the gardenia extract with extremely low concentration has stronger capability of eliminating free radicals.
The astragalus membranaceus contains astragalus polysaccharide, astragalus saponin, flavonoids, amino acids, alkaloids, proteins, vitamins and various trace elements, and the main chemical components of the astragalus membranaceus are triterpenoid saponins, flavonoids, polysaccharides and other components, wherein the astragalus polysaccharide not only can increase the level of superoxide dismutase reduced along with the increase of age and reduce the generation of free radicals, but also can reduce the content of peroxide in blood plasma, so that the formation of lipofuscin is reduced, and even the capability of removing lipofuscin formed in cells is realized, and the astragalus polysaccharide has an excellent improvement effect on the aging phenomena such as pigment deposition, free radical increase and the like caused by photoaging and self-oxidation.
The glossy privet fruit mainly contains terpenes, flavonoids, phenoxyl glycosides, fatty acids and other components, and the glossy privet fruit has the effect of delaying organism aging, because oleanolic acid, polysaccharide and iridoid chemicals in the glossy privet fruit extract can better eliminate free radicals, improve the defense capacity of an organism to the free radicals and inhibit erythrocyte hemolysis induced by the free radicals, thereby prolonging the survival time of cells.
The traditional Chinese medicine composition contains multiple anti-aging ingredients, the four traditional Chinese medicines are compatible according to the optimal proportion, the component formula is reasonable, the active ingredients supplement each other, the synergy can be realized, the prepared cosmetics are mild in property, and the quick and long-acting anti-aging effect can be generated.
The peony can be 20%, 25%, 30%, 32%, 35%, 38%, 40%, 42%, 45%, 46%, 48% or 50% by mass.
The gardenia can be 10%, 12%, 15%, 18%, 20%, 22%, 25%, 28% or 30% by mass.
The content of the astragalus membranaceus root in percentage by mass can be 10%, 12%, 15%, 18%, 20%, 22%, 25%, 28% or 30% and the like.
The mass percentage content of the glossy privet seed can be 10%, 12%, 15%, 18%, 20%, 22%, 25%, 28% or 30% and the like.
Preferably, the peony is taken from any one or a combination of at least two of peony, peony leaf, peony stem or peony root, for example, peony and peony leaf, peony stem and peony root, peony leaf and peony stem, and the like.
Preferably, the preparation raw materials of the traditional Chinese medicine composition comprise the following components in percentage by mass: 35% of peony, 25% of gardenia, 25% of astragalus membranaceus root and 15% of glossy privet seed.
Preferably, the preparation raw materials of the traditional Chinese medicine composition comprise the following components in percentage by mass: 35% of peony, 20% of gardenia, 20% of astragalus membranaceus root and 25% of glossy privet seed.
In a second aspect, the present invention provides a preparation method of the above-mentioned traditional Chinese medicine composition, comprising the following steps:
(1) pretreating medicinal materials: respectively processing peony, gardenia, astragalus membranaceus root and glossy privet seed, then crushing into powder, and mixing to obtain mixed powder;
(2) primary extraction: adding a first extraction reagent into the mixed powder obtained in the step (1), soaking, and then filtering, concentrating and drying an extracting solution to obtain dry powder;
(3) secondary extraction: and (3) adding a second extraction reagent into the dry powder obtained in the step (2), and performing ultrasonic extraction to obtain a crude extraction solution, thus obtaining the traditional Chinese medicine composition.
In the invention, the specific method for processing peony in step (1) is as follows: washing peony with deionized water, removing root and stem xylem, and keeping outer skin, wherein the outer skin is soaked in 5-20% (such as 5%, 8%, 10%, 12%, 15%, 18% or 20%) ethanol water solution for 1-5 hr (such as 1 hr, 2 hr, 3 hr, 4 hr or 5 hr), and parched, wherein flower and leaf are dried at 40-80 deg.C (such as 40 deg.C, 45 deg.C, 50 deg.C, 55 deg.C, 60 deg.C, 65 deg.C, 70 deg.C, 75 deg.C or 80 deg.C).
Preferably, the specific method for processing gardenia in the step (1) comprises the following steps: cleaning Gardenia with deionized water, and drying at 40-80 deg.C (such as 40 deg.C, 45 deg.C, 50 deg.C, 55 deg.C, 60 deg.C, 65 deg.C, 70 deg.C, 75 deg.C or 80 deg.C).
Preferably, the specific method for processing the astragalus membranaceus roots in step (1) comprises the following steps: washing radix astragali with deionized water, steaming at 60-100 deg.C (60 deg.C, 70 deg.C, 80 deg.C, 90 deg.C or 100 deg.C) for 3-5 hr (such as 3 hr, 3.5 hr, 4 hr, 4.5 hr or 5 hr), drying at 50-80 deg.C (such as 50 deg.C, 55 deg.C, 60 deg.C, 65 deg.C, 70 deg.C, 75 deg.C or 80 deg.C) for 5-10h (such as 5h, 6h, 7h, 8h or 10 h), drying at 20-30 deg.C (such as 20 deg.C, 21 deg.C, 22 deg.C, 24 deg.C, 26 deg.C, 28 deg.C or 30 deg.C) for 1-12h (1h, 2h, 4h, 6h, 8h, 10h or 12 h), and drying at 50-80 deg.C (such as 50 deg.C, 55 deg.C, 65 deg.C, 70 deg.C, 75 deg.C or 80 deg.C) for 5-10h (such as 5h, 6h, 7h, 8h, 9h or 10 h).
Preferably, the specific method for processing the glossy privet fruit seeds in the step (1) comprises the following steps: cleaning glossy privet seed with deionized water, removing impurities and fruit stem, sealing in 1-10% (1%, 2%, 3%, 4%, 5%, 6%, 8% or 10%) acetic acid water solution, heating over water for 3-8 hr (3 hr, 4 hr, 5 hr, 6 hr, 7 hr or 8 hr, etc.), and drying at 50-80 deg.C (such as 50 deg.C, 55 deg.C, 60 deg.C, 65 deg.C, 70 deg.C, 75 deg.C or 80 deg.C).
After the raw materials for preparing the traditional Chinese medicine composition are respectively processed by the special processing mode, the content of active ingredients in the raw materials can be greatly improved, and the common toxic and side effects of the traditional Chinese medicine can be effectively reduced; meanwhile, the bitter and cold properties of the peony, the gardenia and the astragalus membranaceus are also well improved, so that the medicinal properties of the peony, the gardenia and the astragalus membranaceus are better exerted.
Preferably, the particle size of the powder of step (1) is 60-100 mesh, such as 60 mesh, 65 mesh, 70 mesh, 75 mesh, 80 mesh, 85 mesh, 90 mesh, 95 mesh or 100 mesh, etc.
In the present invention, the first extraction solvent in step (2) is 40-80% ethanol aqueous solution, such as 40%, 45%, 50%, 60%, 70%, or 80%.
Preferably, the mass ratio of the first extraction solvent to the mixed powder in step (2) is (8-20):1, such as 8:1, 10:1, 12:1, 14:1, 15:1, 16:1, 17:1, 18:1 or 20:1, etc.
Preferably, the temperature of the soaking in step (2) is 40-70 deg.C, such as 40 deg.C, 45 deg.C, 50 deg.C, 55 deg.C, 60 deg.C, 65 deg.C or 70 deg.C.
Preferably, the soaking time in step (2) is 2-6 times, such as 2 times, 3 times, 4 times, 5 times, 6 times and the like, each time for 2-3h, such as 2h, 2.2h, 2.4h, 2.5h, 2.6h, 2.8h, 3h and the like.
Preferably, the concentration of step (2) is concentration under reduced pressure.
Preferably, the drying in step (2) is vacuum drying, and the drying temperature is 40-70 deg.C, such as 40 deg.C, 45 deg.C, 50 deg.C, 55 deg.C, 60 deg.C, 65 deg.C or 70 deg.C.
In the present invention, the second extraction reagent in step (3) is deionized water.
Preferably, the mass ratio of the second extraction solvent to the dry powder in step (3) is (10-20: 1, such as 10:1, 11:1, 12:1, 14:1, 15:1, 16:1, 18:1, 19:1 or 20:1, etc.
Preferably, the power of the ultrasonic extraction in the step (3) is 30-80KHz, such as 30KHz, 40KHz, 50KHz, 60KHz, 70KHz or 80 KHz.
Preferably, the temperature of the ultrasonic extraction in step (3) is 40-80 deg.C, such as 40 deg.C, 45 deg.C, 50 deg.C, 55 deg.C, 60 deg.C, 65 deg.C, 70 deg.C, 75 deg.C or 80 deg.C.
Preferably, the time of the ultrasonic extraction in the step (3) is 30-100min, such as 30min, 40min, 50min, 60min, 70min, 80min, 90min or 100 min.
In a third aspect, the invention provides a Chinese medicinal composition concentrated solution, which is obtained by purifying and concentrating the Chinese medicinal composition.
In a fourth aspect, the present invention provides a preparation method of the above concentrated solution of a Chinese medicinal composition, comprising the following steps:
purifying: filtering the traditional Chinese medicine composition by using a microporous filter membrane to obtain a filtrate, and eluting the filtrate by using macroporous resin to obtain an eluent;
(II) concentrating: concentrating the eluate obtained in step (I).
Preferably, the pore size of the microfiltration membrane according to step (I) is 0.2 to 0.5. mu.m, such as 0.2. mu.m, 0.25. mu.m, 0.3. mu.m, 0.4. mu.m, 0.45. mu.m or 0.5. mu.m.
Preferably, the model of the macroporous resin column in the step (I) is D101 or AB-8.
Preferably, the eluent used in the elution in step (I) is 70% ethanol water solution.
Preferably, the concentration in step (II) is carried out in a vacuum concentrator at a temperature of 40-80 deg.C, such as 40 deg.C, 45 deg.C, 50 deg.C, 55 deg.C, 60 deg.C, 65 deg.C, 70 deg.C, 75 deg.C or 80 deg.C.
Preferably, the concentration in the step (II) is carried out until the mass ratio of the final mass to the mixed powder in the step (1) is 1 (5-20), such as 1:5, 1:6, 1:8, 1:10, 1:12, 1:15, 1:16, 1:18 or 1: 20.
As a preferred technical scheme of the invention, the preparation method of the traditional Chinese medicine composition concentrated solution comprises the following steps:
(1) pretreating medicinal materials: respectively processing peony, gardenia, astragalus membranaceus root and glossy privet seed, then crushing into powder, and mixing to obtain mixed powder;
(2) primary extraction: adding 40-80% ethanol water solution into the mixed powder obtained in the step (1), wherein the mass ratio of the ethanol water solution to the mixed powder is (8-20): 1; soaking at 40-70 deg.C for 2-6 times, each for 2-3 hr, filtering the extractive solution, concentrating under reduced pressure, and vacuum drying at 40-70 deg.C to obtain dry powder;
(3) secondary extraction: adding deionized water into the dry powder obtained in the step (2), wherein the mass ratio of the deionized water to the dry powder is (10-20): 1; performing ultrasonic extraction at 40-80 deg.C for 30-100min with ultrasonic power of 30-80KHz to obtain crude extract to obtain the Chinese medicinal composition;
(4) and (3) purification: filtering the Chinese medicinal composition obtained in step (3) with 0.2-0.5 μm microporous membrane to obtain filtrate, and eluting the filtrate with macroporous resin D101 or AB-8 to obtain 70% ethanol water solution as eluent;
(5) concentration: and (3) concentrating the eluent obtained in the step (4) in a vacuum concentrator at 40-80 ℃ until the mass ratio of the final mass to the mixed powder in the step (1) is 1 (5-20).
In a fifth aspect, the present invention provides a cosmetic, wherein the raw material composition of the cosmetic comprises the above traditional Chinese medicine composition or comprises the above traditional Chinese medicine composition concentrated solution.
Preferably, the cosmetic is a lotion, essence, or a mask.
Preferably, the amount of the traditional Chinese medicine composition or the traditional Chinese medicine composition concentrated solution added in the cosmetic is 0.1-30%, such as 0.1%, 0.5%, 1%, 2%, 5%, 8%, 10%, 15%, 20% or 30%, etc., preferably 0.5-20%.
Preferably, the raw material composition of the cosmetic further comprises an additive, wherein the additive comprises any one of a solvent, an emulsifier, a preservative or a thickener or a combination of at least two of the two, such as the solvent and the emulsifier, the preservative and the thickener, the emulsifier and the preservative, and the like.
Compared with the prior art, the invention has the following beneficial effects:
the traditional Chinese medicine composition provided by the invention is prepared by matching the four traditional Chinese medicines according to the optimal proportion, is reasonable in component formula, and has the advantages that each active ingredient supplements each other, can realize synergistic interaction, can remove superoxide anion free radicals and hydroxyl free radicals, and has a remarkable anti-aging effect. The Chinese medicinal composition is applied to cosmetics, so that the prepared product has stable properties, and has good effects of moisturizing, resisting wrinkles, preventing pigmented spots and the like.
Detailed Description
To further illustrate the technical means and effects of the present invention, the following further describes the technical solution of the present invention with reference to the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.
Example 1
The embodiment provides a traditional Chinese medicine composition concentrated solution with an anti-aging effect, and the traditional Chinese medicine composition concentrated solution is prepared from the following raw materials in percentage by mass: 35% of peony, 25% of gardenia, 25% of astragalus membranaceus root and 15% of glossy privet seed.
The preparation method comprises the following steps:
processing peony, gardenia, astragalus membranaceus root and glossy privet seed as follows:
cleaning peony with deionized water, removing woody part of root and stem, and keeping outer skin, soaking the outer skin in 10% ethanol water solution for 4 hr, parching, and drying flower and leaf at 60 deg.C; cleaning gardenia with deionized water, and drying at 60 ℃; washing radix astragali with deionized water, steaming at 80 deg.C for 3 hr, drying at 70 deg.C for 8 hr, drying at 25 deg.C for 10 hr, and drying at 70 deg.C for 8 hr; cleaning glossy privet seed with deionized water, removing impurities and fruit stem, sealing in 4% acetic acid water solution, heating over water for 7 hr, and drying at 60 deg.C.
Respectively processing peony, gardenia, astragalus membranaceus root and glossy privet seed, then crushing into powder, and mixing to obtain mixed powder; adding 60% ethanol water solution into the mixed powder at a mass ratio of 15:1, soaking at 60 deg.C for 3 times, each for 2 hr, filtering the extractive solution, concentrating under reduced pressure, and vacuum drying at 60 deg.C to obtain dried powder; adding deionized water into the obtained dry powder at a mass ratio of 15:1, and performing ultrasonic extraction at 60 deg.C for 45min with ultrasonic power of 55KHz to obtain crude extract; filtering with 0.2 μm microporous membrane to obtain filtrate, eluting with D101 macroporous resin to obtain 70% ethanol water solution as eluent; concentrating the obtained eluent in a vacuum concentrator at 40 ℃, and concentrating until the mass ratio of the final mass to the mixed powder is 1:10 to obtain the traditional Chinese medicine composition concentrated solution (I).
Example 2
The embodiment provides a traditional Chinese medicine composition concentrated solution with an anti-aging effect, and the traditional Chinese medicine composition concentrated solution is prepared from the following raw materials in percentage by mass: 35% of peony, 20% of gardenia, 20% of astragalus membranaceus root and 25% of glossy privet seed.
The preparation method comprises the following steps:
processing peony, gardenia, astragalus membranaceus root and glossy privet seed as follows:
cleaning peony with deionized water, removing woody part of root and stem, and keeping outer skin, soaking the outer skin in 15% ethanol water solution for 5 hr, parching, and drying flower and leaf at 70 deg.C; cleaning gardenia with deionized water, and drying at 70 ℃; washing radix astragali with deionized water, steaming at 60 deg.C for 5 hr, drying at 60 deg.C for 6 hr, drying at 25 deg.C for 5 hr, and drying at 60 deg.C for 5 hr; cleaning fructus Ligustri Lucidi with deionized water, removing impurities and fruit stem, sealing in 8% acetic acid water solution, heating over water for 5 hr, and drying at 70 deg.C.
Respectively processing peony, gardenia, astragalus membranaceus root and glossy privet seed, then crushing into powder, and mixing to obtain mixed powder; adding 70% ethanol water solution into the mixed powder at a mass ratio of 10:1, soaking at 70 deg.C for 3 hr for 4 times, filtering the extractive solution, concentrating under reduced pressure, and vacuum drying at 65 deg.C to obtain dried powder; adding deionized water into the obtained dry powder at a mass ratio of 10:1, and performing ultrasonic extraction at 70 deg.C for 80min with ultrasonic power of 70KHz to obtain crude extract; filtering with 0.5 μm microporous membrane to obtain filtrate, eluting with macroporous resin AB-8 to obtain 70% ethanol water solution as eluent; concentrating the obtained eluent in a vacuum concentrator at 60 ℃, and concentrating until the mass ratio of the final mass to the mixed powder is 1:15 to obtain the traditional Chinese medicine composition concentrated solution (II).
Example 3
The embodiment provides a traditional Chinese medicine composition concentrated solution with an anti-aging effect, and the traditional Chinese medicine composition concentrated solution is prepared from the following raw materials in percentage by mass: 45% of peony, 25% of gardenia, 15% of astragalus membranaceus root and 15% of glossy privet seed.
The preparation method comprises the following steps:
processing peony, gardenia, astragalus membranaceus root and glossy privet seed as follows:
cleaning peony with deionized water, removing woody part of root and stem, and keeping outer skin, soaking the outer skin in 20% ethanol water solution for 5 hr, parching, and drying flower and leaf at 80 deg.C; cleaning gardenia with deionized water, and drying at 75 ℃; washing radix astragali with deionized water, steaming at 100 deg.C for 4 hr, drying at 80 deg.C for 9 hr, drying at 25 deg.C for 12 hr, and drying at 80 deg.C for 10 hr; cleaning glossy privet seed with deionized water, removing impurities and fruit stem, sealing in 10% acetic acid water solution, heating over water for 4 hr, and drying at 80 deg.C.
Respectively processing peony, gardenia, astragalus membranaceus root and glossy privet seed, then crushing into powder, and mixing to obtain mixed powder; adding 75% ethanol water solution into the mixed powder at a mass ratio of 19:1, soaking at 50 deg.C for 3 hr for 4 times, filtering the extractive solution, concentrating under reduced pressure, and vacuum drying at 50 deg.C to obtain dried powder; adding deionized water into the obtained dry powder at a mass ratio of 20:1, and performing ultrasonic extraction at 60 deg.C for 100min with ultrasonic power of 40KHz to obtain crude extract; filtering with 0.4 μm microporous membrane to obtain filtrate, eluting with D101 macroporous resin to obtain 70% ethanol water solution as eluent; concentrating the obtained eluate in a vacuum concentrator at 80 deg.C until the mass ratio of the final mass to the mixed powder is 1:20 to obtain the Chinese medicinal composition concentrated solution (III).
Example 4
The embodiment provides a traditional Chinese medicine composition concentrated solution with an anti-aging effect, and the traditional Chinese medicine composition concentrated solution is prepared from the following raw materials in percentage by mass: 25% of peony, 15% of gardenia, 30% of astragalus membranaceus root and 30% of glossy privet seed.
The preparation method comprises the following steps:
processing peony, gardenia, astragalus membranaceus root and glossy privet seed respectively in the same manner as in example 1.
Respectively processing peony, gardenia, astragalus membranaceus root and glossy privet seed, then crushing into powder, and mixing to obtain mixed powder; adding 75% ethanol water solution into the mixed powder at a mass ratio of 19:1, soaking at 50 deg.C for 3 hr for 4 times, filtering the extractive solution, concentrating under reduced pressure, and vacuum drying at 50 deg.C to obtain dried powder; adding deionized water into the obtained dry powder at a mass ratio of 20:1, and performing ultrasonic extraction at 60 deg.C for 100min with ultrasonic power of 40KHz to obtain crude extract; filtering with 0.4 μm microporous membrane to obtain filtrate, eluting with D101 macroporous resin to obtain 70% ethanol water solution as eluent; concentrating the obtained eluent in a vacuum concentrator at 80 ℃, and concentrating until the mass ratio of the final mass to the mixed powder is 1:20 to obtain the traditional Chinese medicine composition concentrated solution (IV).
Comparative examples 1 to 4
Comparative examples 1-4 provide 4 single-component herbal extract concentrates, comparative example 1 (peony extract), comparative example 2 (gardenia extract), comparative example 3 (astragalus membranaceus root extract), and comparative example 4 (ligustrum lucidum extract), respectively. The preparation method is the same as that of example 1.
Comparative example 5
The comparative example provides an unprocessed traditional Chinese medicine composition concentrated solution, which is prepared from the following raw materials in percentage by mass: 35% of peony, 25% of gardenia, 25% of astragalus membranaceus root and 15% of glossy privet seed.
The preparation method comprises the following steps:
cleaning peony, gardenia, astragalus membranaceus root and glossy privet seed with deionized water, drying, crushing into powder, and mixing to obtain mixed powder; adding deionized water into the mixed powder at a mass ratio of 15:1, and performing ultrasonic extraction at 60 deg.C for 45min with ultrasonic power of 55KHz to obtain crude extract; the subsequent operations were kept the same as in example 1 to obtain the Chinese medicinal composition concentrate (V).
Example 5
The embodiment provides a mask with an anti-aging effect, which is prepared from raw materials containing a traditional Chinese medicine composition concentrated solution (I), and the components of the mask are shown in Table 1.
TABLE 1
The preparation method comprises the following steps:
(1) dispersing xanthan gum and sodium hyaluronate in the phase A in glycerol, then putting the dispersed xanthan gum and sodium hyaluronate into an emulsifying pot, directly putting EDTA disodium and hydroxyethyl cellulose into the emulsifying pot, heating to 80 ℃, and stirring and dispersing the mixture until the mixture is uniform;
(2) pre-heating p-hydroxyacetophenone in the phase B in butanediol to dissolve and be transparent, adding the p-hydroxyacetophenone into an emulsifying pot at the temperature of 60 ℃, and stirring and dispersing the p-hydroxyacetophenone uniformly;
(3) cooling to 45 ℃, adding the phase C, and stirring and dispersing until the phase C is completely dissolved;
(4) cooling to 37 ℃, stirring evenly, vacuumizing, defoaming, filtering with 200 meshes and discharging.
Comparative examples 6 to 10
Comparative examples 6 to 10 provide 5 kinds of facial masks having anti-aging effects, which were prepared from the same raw materials as in example 5 except that "the herbal composition concentrate (i)" was replaced with "the peony extract", "the gardenia extract", "the astragalus membranaceus root extract", "the glossy privet fruit extract" prepared in comparative examples 1 to 4, and "the herbal composition concentrate (iv)" prepared in comparative example 5, respectively, and the other materials were the same, and the preparation methods were the same.
Example 6
The embodiment provides an essence with an anti-aging effect, which is prepared from raw materials including a traditional Chinese medicine composition concentrated solution (I), and the components of the essence are shown in Table 2.
TABLE 2
The preparation method comprises the following steps:
(1) stirring sodium hyaluronate, xanthan gum and glycerol until the sodium hyaluronate, the xanthan gum and the glycerol are uniformly dispersed, and preparing carbomer into a water dispersion with the mass fraction of 1%;
(2) adding A phase raw materials including sweet almond oil, squalane, methyl hydroxybenzoate, propyl hydroxybenzoate, tween-20 and polysorbate-60 into an oil phase pot, heating to 80 deg.C, stirring, and adding cyclopenta dimethyl siloxane before emulsification;
(3) putting the glycerol, the sodium hyaluronate, the xanthan gum and the EDTA disodium pretreated in the step (1) into an emulsifying pot, heating to 80 ℃, and stirring until the materials are completely dissolved;
(4) rapidly pumping the phase A into an emulsifying pot, homogenizing and emulsifying at the speed of 3000-5000rpm for 5-10min after the addition is finished, continuously stirring at the speed of 1000-2000rpm after the emulsification is finished, and cooling;
(5) cooling to 65 ℃, adding the carbomer water dispersion liquid with the mass fraction of 1 percent prepared in the step (1), and homogenizing for 2 minutes at the speed of 3000-;
(6) cooling to 45 ℃, adding the phase C, and stirring until the phase C is dissolved uniformly;
(7) and (4) after the temperature is reduced to 35 ℃, carrying out vacuum defoamation, filtering by a 200-mesh sieve and discharging.
Comparative example 11
This example provides a common essence, which is prepared from the same raw materials as those in example 6 except that the concentrated herbal composition (i) is removed, and the preparation method is the same.
Comparative example 12
This example provides an essence, which is prepared from the same raw materials as in example 6 except that the "concentrated solution of Chinese medicinal composition (i)" is replaced with the "concentrated solution of Chinese medicinal composition (iv)" prepared in comparative example 5, and the rest are the same, and the preparation method is the same.
The products obtained in examples 1 to 6 and comparative examples 1 to 12 were subjected to the following test tests, respectively:
example 7
Evaluation test of antioxidant efficacy
The products from examples 1-4 and comparative examples 1-5 were prepared as 1% sample solutions with deionized water and tested as follows:
(1) evaluation test of superoxide anion radical scavenging ability
4.5mL of 0.05mol/L Tris-HCl buffer solution with pH 8.2 was preheated in a 25 ℃ water bath for 20 min. Then adding 1mL of sample solution and 0.4mL of 25mol/L pyrogallol solution, mixing uniformly, reacting in a water bath at 25 ℃ for 5min, and adding 1mL of 8mol/L HCl to stop the reaction. Absorbance values were measured at 299nm with Tris-HCl buffer as reference. The blank control group replaced the sample solution with 1mL of the solvent of the sample solution.
The calculation formula of the superoxide anion radical clearance is as follows:
superoxide anion radical scavenging rate (%) [1- (A2/A1) ] x 100%
Wherein A1 is the absorbance value of the blank control group and A2 is the absorbance value of the sample group. The test results are shown in table 3.
(2) Evaluation test of hydroxyl radical scavenging ability
2mmol/L FeSO are added into a 25mL cuvette in turn4 3mL，1mmol/L H2O23mL, shaking up, then adding 3mL of salicylic acid with the concentration of 6mmol/L, shaking up, heating in a water bath at 37 ℃ for 15min, taking out, and measuring the absorbance; adding sample solutions with certain concentrations, shaking, heating in water bath for 15min, taking out, and measuring absorbance.
The hydroxyl radical clearance is calculated as follows:
hydroxyl radical clearance (%) ═ a1-A2-(A1-A3)]/A1×100％
In the formula, A1、A2、A3Respectively the absorbance value of the reaction system before adding the sample, the absorbance value of the system after removing hydroxyl radicals from the sample, and the absorbance value of the system after removing hydroxyl radicals from the sample solventAbsorbance values of free radical post system. The test results are shown in table 3.
TABLE 3
| Test items | Superoxide anion radical scavenging ratio (%) | Hydroxyl radical scavenging rate (%) |
| Example 1 | 99.33 | 93.87 |
| Example 2 | 92.22 | 93.59 |
| Example 3 | 92.35 | 91.47 |
| Example 4 | 92.57 | 91.48 |
| Comparative example 1 | 43.65 | 44.29 |
| Comparative example 2 | 41.54 | 45.83 |
| Comparative example 3 | 52.18 | 41.27 |
| Comparative example 4 | 17.53 | 16.72 |
| Comparative example 5 | 60.23 | 65.92 |
According to the test results, the products prepared in examples 1 to 4 have a superoxide anion removal rate of more than 92% and a hydroxyl radical removal rate of more than 91%, which indicates that the traditional Chinese medicine composition with the anti-aging effect has a remarkable free radical removal and anti-oxidation effect. The clearance rate of the single traditional Chinese medicine to free radicals in the comparative example is not ideal, which shows that the synergistic effect among the compositions is obvious, and the clearance rate of the traditional Chinese medicine composition to superoxide anions and hydroxyl free radicals is higher than that of the single traditional Chinese medicine, so that the traditional Chinese medicine composition has good anti-aging effect. Meanwhile, the traditional Chinese medicine composition which is not processed in the comparative example 5 has the clearance rate of only 60% of free radicals and has no obvious clearance capacity of hydroxyl free radicals, which shows that the traditional Chinese medicine composition which is processed in a special way has stronger capacity of clearing free radicals and resisting oxidation.
Example 8
Skin moisture content and skin Water loss (TEWL) test
The cosmetics prepared in examples 5 to 6 and comparative examples 6 to 12 were selected for skin moisture content and skin moisture loss (TEWL) tests, which are world-recognized
Method-capacitance method, the skin water loss TEWL is a very important parameter for assessing the function of the skin water protective layer. As the skin ages, the skin becomes more and more sensitive,the water retention capacity of the skin gradually decreases, the more easily the skin dries, and the lower the TEWL value for skin water loss. The test instruments used were a skin moisture Corneometer test probe and a skin moisture loss Tewameter TM300 test probe (Courage + Khazaka, Germany).
The test method specifically comprises the following steps:
140 aged skin-aging women aged 35-55 years are selected and randomly divided into 7 groups, and each group is 20. Any product (cosmetics or external medicines or internal health care products) cannot be used for 15 days before the tested part. Before the test, the subject was asked to wash his face and sit still for 30min in a constant temperature and humidity room at a temperature of 22. + -. 1 ℃ and a humidity of 50. + -. 5% 2 hours after washing, and kept in a relaxed state.
In the experiment, 5 groups are selected according to a random table, and half face test is carried out, wherein one side is example 4 containing traditional Chinese medicine extracts, and the other side is comparative examples 6-10; the other 2 groups had one side of example 5 containing herbal extracts and the other side of comparative examples 11-12; the left and right nasolabial sulcus parts of the volunteer were selected for skin moisture content test and moisture loss (TEWL) test, and the average of 3 values was taken as the nasolabial sulcus moisture content data (D0) and the nasolabial sulcus skin moisture loss data (TEWL) (D0). After the test is finished, the volunteers need to use the product according to the regulations, one side face is coated with one product, and the other side face is coated with the other product. When in use, the right face is smeared by the right hand, the left face is smeared by the left hand, cross contamination is forbidden, product smearing is carried out 2 times a day in the morning and at night, the trial is continuously carried out for 28 days, and the 14 th (D14) and 28 th (D28) days of the trial are required to carry out return visit. The lighting conditions at each time point should remain consistent: the curtain is closed and the lighting is proper. The subject was lying in bed. The angle of bed tilt should be consistent at each point in time. The position of the bed must be noted on the ground to ensure consistency of the positions of D14 and D28. The test of the same volunteer was performed by the same measurement staff. The test results are shown in table 4.
TABLE 4
| Test index | D14 moisture content | D28 moisture content | D14TEWL | D28TEWL |
| Example 5 | 20.28 | 29.21 | -20.39 | -33.97 |
| Example 6 | 19.73 | 34.92 | -18.85 | -32.33 |
| Comparative example 6 | 10.32 | 14.53 | -11.02 | -13.96 |
| Comparative example 7 | 11.25 | 14.01 | -10.99 | -14.67 |
| Comparative example 8 | 12.00 | 13.69 | -10.81 | -12.58 |
| Comparative example 9 | 11.56 | 14.26 | -11.29 | -14.91 |
| Comparative example 10 | 10.89 | 13.78 | -8.65 | -10.11 |
| Comparative example 11 | 11.31 | 12.25 | -5.94 | -9.21 |
| Comparative example 12 | 13.57 | 16.51 | -10.38 | -13.92 |
According to the test results, the repair effect of the essence containing the traditional Chinese medicine composition with the anti-aging effect on the skin water retention capacity is more obvious than that of the essence containing single traditional Chinese medicine, the facial mask containing the unprocessed traditional Chinese medicine composition and the essence not containing the traditional Chinese medicine composition, and the repair effect of the essence containing the unprocessed traditional Chinese medicine composition is slightly better than that of the essence not containing the traditional Chinese medicine composition on the skin water retention capacity, but is far lower than that of the essence containing the processed traditional Chinese medicine composition.
Example 9
VISIA skin analysis evaluation experiment
The VISIA skin image analyzer adopts a multi-spectral image technology, can shoot from three angles of a front side, a left side and a right side through a 1200 ten thousand pixel camera, and forms images in three light sources of standard white light, cross-section polarized light and ultraviolet light for three times, so that the VISIA facial image analysis system can systematically analyze pigment spots, textures, pores, wrinkles, ultraviolet light spots, stable spots, red areas and purple on the facial skin of a subject, and provides accurate and quantitative analysis basis for the skin. The change condition of the skin property is observed by recording pigment spots, wrinkles and textures of the face of a subject before and after the test, wherein the absolute value of the value represents the area and the strength of the detected value of the skin characteristic, so that the data obtained by the absolute value (the average value of the test results of the front side, the left side and the right side) is selected as an analysis standard and a data statistical basis. All data of each subject are input into a computer, the measured results are compared, and the skin characteristics and various index data of the subjects are analyzed through statistical treatment. The test apparatus used for the experiment was the VISIA facial image analysis system (canfeld technologies, usa).
The test method specifically comprises the following steps:
30 women of aged skin aged 30 to 40 years old were selected and randomly divided into 3 groups of 10 persons each using example 6, comparative example 11 and comparative example 12, respectively. The subjects continued to use, with product application 2 times a day, morning and evening, on 28 consecutive days of trial, and required visits to day 14 (D14) and day 28 (D28) of the trial. The test of the same subject is performed by the same measuring person.
Any product (cosmetics or external medicines or internal health care products) cannot be used for 15 days before the tested part. After the face is thoroughly cleaned by using a uniform facial cleaning product, the face is lightly wiped by using a paper towel, the face of a tester is photographed by using a VISIA facial image analyzer after the room temperature is 22 +/-2 ℃ and the relative humidity is 40 +/-2% for 15 minutes, and the analysis area is prevented from being interfered by a light reflecting area and hair as much as possible. The face (including the front, the left side and the right side) of the subject is photographed, the obtained absolute score is subjected to data statistics, and the detection data of pigment spots, textures, wrinkles, pores, red areas and purpurins of the face of the subject are recorded. The test results are shown in table 5.
TABLE 5
From the above test results, it can be seen that example 6 has a significant improvement effect on pigmented spots, wrinkles and texture of the subjects, while comparative example 11 has no improvement effect and comparative example 12 has a slight effect. The long-term use of the essence added with the traditional Chinese medicine composition with the anti-aging effect can achieve good effects of removing wrinkles, fading pigmented spots and smoothing textures.
Example 10
Human body trial evaluation experiment
The facial masks prepared in the embodiment 5 and the comparative examples 6-10 are selected to be used for human trial evaluation tests, and the moisture retention, the glossiness, the compactness and the anti-wrinkle effect of the facial masks are evaluated by volunteers in the using process, wherein the maximum score is 5, the minimum score is 1, and any value between 1 and 5 can be taken. The test results are shown in table 6.
TABLE 6
| Evaluation results | Examples5 | Comparative example 6 | Comparative example 7 | Comparative example 8 | Comparative example 9 | Comparative example 10 |
| Moisture retention | 4.50 | 4.20 | 4.35 | 4.05 | 3.95 | 4.15 |
| Degree of gloss | 3.55 | 3.25 | 3.40 | 3.35 | 3.65 | 3.50 |
| Compactness degree | 3.55 | 3.60 | 3.45 | 3.15 | 3.05 | 3.30 |
| Anti-wrinkle effect | 3.45 | 3.05 | 3.00 | 3.35 | 3.65 | 3.75 |
According to the test results, the examples have obvious effects on improving moisture retention, glossiness, firmness and anti-wrinkle effects, and show that the facial mask containing the anti-aging traditional Chinese medicine composition has good anti-aging and nourishing effects, and can restore the dry, dark and loose skin to a healthy state of moist, smooth, glossy, fine, firm and full.
The applicant states that the present invention illustrates the traditional Chinese medicine composition with anti-aging effect and the preparation method and application thereof through the above embodiments, but the present invention is not limited to the above embodiments, that is, it does not mean that the present invention must be implemented by the above embodiments. It should be understood by those skilled in the art that any modification of the present invention, equivalent substitutions of the raw materials of the product of the present invention, addition of auxiliary components, selection of specific modes, etc., are within the scope and disclosure of the present invention.
The preferred embodiments of the present invention have been described in detail, however, the present invention is not limited to the specific details of the above embodiments, and various simple modifications may be made to the technical solution of the present invention within the technical idea of the present invention, and these simple modifications are within the protective scope of the present invention.
It should be noted that the various technical features described in the above embodiments can be combined in any suitable manner without contradiction, and the invention is not described in any way for the possible combinations in order to avoid unnecessary repetition.
Claims (19)
1. The traditional Chinese medicine composition is characterized by being prepared from the following raw materials in percentage by mass: 20-50% of peony, 10-30% of gardenia, 10-30% of astragalus membranaceus root and 10-30% of glossy privet fruit;
the traditional Chinese medicine composition is prepared by a preparation method comprising the following steps:
(1) pretreating medicinal materials: respectively processing peony, gardenia, astragalus membranaceus root and glossy privet fruit, then crushing into powder, and mixing to obtain mixed powder;
(2) primary extraction: adding 40-80% ethanol water solution into the mixed powder obtained in the step (1), wherein the mass ratio of the ethanol water solution to the mixed powder is (8-20): 1; soaking at 40-70 deg.C for 2-6 times, each for 2-3 hr, filtering the extractive solution, concentrating under reduced pressure, and vacuum drying at 40-70 deg.C to obtain dry powder;
(3) secondary extraction: adding deionized water into the dry powder obtained in the step (2), wherein the mass ratio of the deionized water to the dry powder is (10-20): 1; performing ultrasonic extraction at 40-80 deg.C for 30-100min with ultrasonic power of 30-80KHz to obtain crude extract to obtain the Chinese medicinal composition;
the specific method for processing the peony in the step (1) comprises the following steps: cleaning peony with deionized water, removing root and stem xylem, and keeping outer skin, soaking the outer skin in 5-20% ethanol water solution for 1-5 hr, parching, and drying flower and leaf at 40-80 deg.C;
the specific method for processing the gardenia in the step (1) comprises the following steps: cleaning gardenia with deionized water, and drying at 40-80 deg.C;
the specific method for processing the astragalus membranaceus roots in the step (1) comprises the following steps: cleaning radix astragali with deionized water, steaming at 60-100 deg.C for 3-5 hr, drying at 50-80 deg.C for 5-10 hr, drying at 20-30 deg.C for 1-12 hr, and drying at 50-80 deg.C for 5-10 hr;
the specific method for processing the glossy privet fruits in the step (1) comprises the following steps: cleaning fructus Ligustri Lucidi with deionized water, removing impurities and fruit stalk, sealing in 1-10% acetic acid water solution, heating over water for 3-8 hr, and drying at 50-80 deg.C.
2. The Chinese medicinal composition of claim 1, wherein the peony is taken from any one or a combination of at least two of peony, peony leaf, peony stem or peony root.
3. The traditional Chinese medicine composition of claim 1, wherein the raw materials for preparing the traditional Chinese medicine composition are prepared from the following components in percentage by mass: 35% of peony, 25% of gardenia, 25% of astragalus membranaceus root and 15% of glossy privet fruit.
4. The traditional Chinese medicine composition of claim 1, wherein the raw materials for preparing the traditional Chinese medicine composition are prepared from the following components in percentage by mass: 35% of peony, 20% of gardenia, 20% of astragalus membranaceus root and 25% of glossy privet fruit.
5. The method for preparing a Chinese medicinal composition according to claim 1, which comprises the following steps:
(1) pretreating medicinal materials: respectively processing peony, gardenia, astragalus membranaceus root and glossy privet fruit, then crushing into powder, and mixing to obtain mixed powder;
(2) primary extraction: adding 40-80% ethanol water solution into the mixed powder obtained in the step (1), wherein the mass ratio of the ethanol water solution to the mixed powder is (8-20): 1; soaking at 40-70 deg.C for 2-6 times, each for 2-3 hr, filtering the extractive solution, concentrating under reduced pressure, and vacuum drying at 40-70 deg.C to obtain dry powder;
(3) secondary extraction: adding deionized water into the dry powder obtained in the step (2), wherein the mass ratio of the deionized water to the dry powder is (10-20): 1; performing ultrasonic extraction at 40-80 deg.C for 30-100min with ultrasonic power of 30-80KHz to obtain crude extract to obtain the Chinese medicinal composition;
the specific method for processing the peony in the step (1) comprises the following steps: cleaning peony with deionized water, removing root and stem xylem, and keeping outer skin, soaking the outer skin in 5-20% ethanol water solution for 1-5 hr, parching, and drying flower and leaf at 40-80 deg.C;
the specific method for processing the gardenia in the step (1) comprises the following steps: cleaning gardenia with deionized water, and drying at 40-80 deg.C;
the specific method for processing the astragalus membranaceus roots in the step (1) comprises the following steps: cleaning radix astragali with deionized water, steaming at 60-100 deg.C for 3-5 hr, drying at 50-80 deg.C for 5-10 hr, drying at 20-30 deg.C for 1-12 hr, and drying at 50-80 deg.C for 5-10 hr;
the specific method for processing the glossy privet fruits in the step (1) comprises the following steps: cleaning fructus Ligustri Lucidi with deionized water, removing impurities and fruit stalk, sealing in 1-10% acetic acid water solution, heating over water for 3-8 hr, and drying at 50-80 deg.C.
6. The method of claim 5, wherein the powder of step (1) has a particle size of 60-100 mesh.
7. A Chinese medicinal composition concentrate, wherein the Chinese medicinal composition concentrate is obtained by purifying and concentrating the Chinese medicinal composition of claim 1.
8. The method for preparing the concentrated solution of the traditional Chinese medicine composition of claim 7, which comprises the following steps:
purifying: filtering the traditional Chinese medicine composition by using a microporous filter membrane to obtain a filtrate, and eluting the filtrate by using macroporous resin to obtain an eluent;
(II) concentrating: concentrating the eluate obtained in step (I).
9. The method for preparing a concentrated solution of a Chinese medicinal composition according to claim 8, wherein the pore size of the microfiltration membrane of step (I) is 0.2 to 0.5 μm.
10. The method for preparing a concentrated solution of a Chinese medicinal composition according to claim 8, wherein the macroporous resin column of step (I) is D101 or AB-8.
11. The method for preparing a concentrated solution of a Chinese medicinal composition according to claim 8, wherein the eluent used in the elution in the step (I) is 70% ethanol aqueous solution.
12. The method of claim 8, wherein the concentration of step (ii) is performed in a vacuum concentrator at a temperature of 40-80 ℃.
13. The method for preparing the concentrated solution of Chinese medicinal composition according to claim 8, wherein the mass ratio of the concentrated solution in the step (II) to the mixed powder in the step (1) is 1 (5-20).
14. The method for preparing the concentrated solution of the traditional Chinese medicine composition according to claim 8, which comprises the following steps:
(1) pretreating medicinal materials: respectively processing peony, gardenia, astragalus membranaceus root and glossy privet fruit, then crushing into powder, and mixing to obtain mixed powder;
(2) primary extraction: adding 40-80% ethanol water solution into the mixed powder obtained in the step (1), wherein the mass ratio of the ethanol water solution to the mixed powder is (8-20): 1; soaking at 40-70 deg.C for 2-6 times, each for 2-3 hr, filtering the extractive solution, concentrating under reduced pressure, and vacuum drying at 40-70 deg.C to obtain dry powder;
(3) secondary extraction: adding deionized water into the dry powder obtained in the step (2), wherein the mass ratio of the deionized water to the dry powder is (10-20): 1; performing ultrasonic extraction at 40-80 deg.C for 30-100min with ultrasonic power of 30-80KHz to obtain crude extract to obtain the Chinese medicinal composition;
(4) and (3) purification: filtering the Chinese medicinal composition obtained in step (3) with 0.2-0.5 μm microporous membrane to obtain filtrate, and eluting the filtrate with macroporous resin D101 or AB-8 to obtain 70% ethanol water solution as eluent;
(5) concentration: and (3) concentrating the eluent obtained in the step (4) in a vacuum concentrator at 40-80 ℃ until the mass ratio of the final mass to the mixed powder in the step (1) is 1 (5-20).
15. A cosmetic is characterized in that the raw material composition of the cosmetic comprises the traditional Chinese medicine composition as claimed in claim 1 or comprises the traditional Chinese medicine composition concentrated solution as claimed in claim 7.
16. The cosmetic of claim 15, wherein the cosmetic is a lotion, a serum, or a facial mask.
17. The cosmetic according to claim 15, wherein the amount of the traditional Chinese medicine composition or the traditional Chinese medicine composition concentrate added in the cosmetic is 0.1-30%.
18. The cosmetic of claim 15, wherein the amount of the Chinese medicinal composition or the Chinese medicinal composition concentrate added to the cosmetic is 0.5-20%.
19. The cosmetic of claim 15, wherein the raw material composition of the cosmetic further comprises an additive, and the additive comprises any one or a combination of at least two of a solvent, an emulsifier, a preservative, or a thickener.
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|---|---|---|---|---|
| CN1113753A (en) * | 1994-06-17 | 1995-12-27 | 殷广全 | Spot curing and beautifying preparation |
| CN105106756A (en) * | 2015-09-23 | 2015-12-02 | 谢桂斌 | Traditional Chinese medicine composition for treating age pigment |
| CN106074826A (en) * | 2016-07-15 | 2016-11-09 | 王旭时 | A kind of multi-functional Chinese medicine preparation and preparation method thereof |
| CN108836933A (en) * | 2018-07-26 | 2018-11-20 | 卜俊超 | A kind of preparation method of the anti-wrinkle Essence containing peony extract |
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2018
- 2018-12-24 CN CN201811585039.9A patent/CN109331074B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1113753A (en) * | 1994-06-17 | 1995-12-27 | 殷广全 | Spot curing and beautifying preparation |
| CN105106756A (en) * | 2015-09-23 | 2015-12-02 | 谢桂斌 | Traditional Chinese medicine composition for treating age pigment |
| CN106074826A (en) * | 2016-07-15 | 2016-11-09 | 王旭时 | A kind of multi-functional Chinese medicine preparation and preparation method thereof |
| CN108836933A (en) * | 2018-07-26 | 2018-11-20 | 卜俊超 | A kind of preparation method of the anti-wrinkle Essence containing peony extract |
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Address after: 510663 No.15, Kelin Road, Guangzhou Science City, Guangzhou high tech Industrial Development Zone, Guangdong Province Patentee after: Guangzhou Huanya cosmetics technology Co.,Ltd. Address before: 510663 No.15, Kelin Road, Guangzhou Science City, Guangzhou high tech Industrial Development Zone, Guangdong Province Patentee before: GUANGZHOU HUANYA COSMETICS TECHNOLOGY Co.,Ltd. |