CN105708759A - Pure natural antioxidant whitening multi-effect face cream with peony seed oil and preparation method thereof - Google Patents

Pure natural antioxidant whitening multi-effect face cream with peony seed oil and preparation method thereof Download PDF

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CN105708759A
CN105708759A CN201610091642.6A CN201610091642A CN105708759A CN 105708759 A CN105708759 A CN 105708759A CN 201610091642 A CN201610091642 A CN 201610091642A CN 105708759 A CN105708759 A CN 105708759A
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skin
face
vitamin
seed oil
peony seed
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CN105708759B (en
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戢小梅
陈法志
李秀丽
陈镇
郑承翔
谭庆
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Wuhan Academy of Agricultural Sciences
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Fruit Tree Research Institute Of Forestry Science Wuhan Academy Of Agricultural Science & Technology
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/92Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
    • A61K8/922Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
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    • A61K8/33Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
    • A61K8/34Alcohols
    • A61K8/345Alcohols containing more than one hydroxy group
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/49Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
    • A61K8/4973Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom
    • A61K8/498Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom having 6-membered rings or their condensed derivatives, e.g. coumarin
    • AHUMAN NECESSITIES
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    • A61K8/96Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q17/00Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
    • A61Q17/04Topical preparations for affording protection against sunlight or other radiation; Topical sun tanning preparations
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin
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    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
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    • A61Q19/02Preparations for care of the skin for chemically bleaching or whitening the skin
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
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    • A61Q19/08Anti-ageing preparations
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    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K2800/00Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
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    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/59Mixtures
    • A61K2800/592Mixtures of compounds complementing their respective functions
    • A61K2800/5922At least two compounds being classified in the same subclass of A61K8/18

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Abstract

The invention discloses a pure natural antioxidant whitening multi-effect face cream with peony seed oil and a preparation method thereof. The face cream is prepared from the peony seed oil, small-molecule squalane, olive emulsifying wax, rose hydrolat, vitamin E, vitamin B5, vitamin B3, fucoge, full-effect exopolysaccharides, glabridin, turmeric extractive, pepper extractive, ginger extractive, cananga odorata hydrolat, deionized water, pentanediol, 1,3butanediol and an antibacterial agent. The pure natural antioxidant whitening multi-effect face cream can effectively moisten face skin, is good in affinity with skin cells and strong in permeability, can be easily absorbed and utilized by face kin, can comprehensively provide various kinds of nutrition needed by face skin, and can rapidly repair damaged cells, recover face skin cell activity, smooth wrinkles, delay face aging, keep the skin color white permanently, enable the face to be white, tender and nutritious and prevent face skin aging.

Description

A kind of pure natural antioxidation whitening multiple-effect washing cream containing peony seed oil and preparation method thereof
Technical field
The invention belongs to daily cosmetics technical field, relate to the compositions that a kind of anti-skin oxidative is old and feeble and suppresses melanin to be formed, particularly relate to a kind of pure natural antioxidation whitening multiple-effect washing cream containing peony seed oil and preparation method thereof.
Background technology
Skin is the outermost tissue of human body, and wherein skin of face is again that human body the most intuitively, the most easily exposes, and is again easily the oldest and the most feeble position simultaneously.With the passing of the years, skin is slowly aging, this process be concealment, progressive, be organism inevitable law of nature.With age, the loss of moisture in human body, support the elasticated net fracture of skin, skin follows the string and gloss, wrinkle, dry hydropenia, pigment excess deposition etc. occurs.Oxidation is one of biggest threat causing skin aging, the free radical causes of senescence of D.Harman, think that human senility is to be caused by the illeffects of the free radical produced in cell normal metabolic processes, free radical excessive in skin cell tissue there will be accumulation and with the lipid material generation Oxidation in cell membrane, cause the damage of cell membrane, thus produce the intense darkness without light pool of complexion, the oxidative phenomena such as hydropenia dries.Therefore one of important means resisting aging is to remove free radical excessive in human body.
Tryrosinase is widely present in human body, is the key enzyme of B16 cell.Skin Bai Bubai, depends primarily on the ability of melanocyte synthesis of melanin.At the basal layer of epidermis iuntercellular of people, being dispersed with melanocyte, oxidizing tyrosine can be become polysaccharide by the tryrosinase that it contains, middle then through a series of metabolic process, finally just can generate melanin.Melanin generates more many, and skin is more pitch-dark;Otherwise, then skin is more pale.Therefore, it is suppressed that tyrosinase activity is effectively to reduce a kind of approach that melanin generates.
Facial cream is the basis most important step of skin protection, and whitening in facial cream, anti-ageing effective ingredient of waiting for a long time can better by percutaneous absorption.Along with improving constantly of modern life level, people increasingly pay attention to skin nursing, and the use of facial cream is also more and more extensive, and the requirement of product composition is also more and more higher.The facial cream sold in the market is of a great variety, is wherein no lack of defying age and whitening product.But, it should be noted that, on the one hand currently marketed facial cream adopts mineral waxiness, synthetic ester oil, mineral wet goods mostly, adds artificial color, spice simultaneously, and some does not stint interpolation hormonal components to realize skin-improving benefits in a short time, whitening product heavy metals exceeding standard etc., not only these compositions can not nourish skin, also can damage face skin, affect the metabolism of face skin, bring various skin problem, body is constituted potential eutrophication;Especially anti-aging product effect is single for the skin-protection product occurred on the market on the other hand at present, can only moisturizing or smoothing wrinkle except speckle, but can not reach moisturizing, moisturizing, prevention pigmentation simultaneously and reduce the multiple-effect protective effect of wrinkle.
Summary of the invention
In order to overcome the shortcomings and deficiencies of prior art, an object of the present invention is in that to provide a kind of compositions integrating moisturizing, moisturizing, antioxidation, prevention pigmentation and the anti aging effect to uvioresistant, especially provides a kind of pure natural antioxidation whitening multiple-effect washing cream containing peony seed oil and preparation method thereof.
In order to realize the purpose of the present invention, inventor is studied by lot of experiments, is finally obtained the technical scheme of the following pure natural antioxidation whitening multiple-effect washing cream containing peony seed oil:
A kind of all natural ingredients compositions delaying face skin old and feeble, it is by peony seed oil, little molecule squalane, excellent olive emulsifing wax, MEIGUI CHUNLU, vitamin E, vitamin B5, vitamin B3, biological polyoses glue, entirely imitate extracellular polysaccharide, glabridin, Rhizoma Curcumae Longae extract, Fructus Piperis extract, Radix Ginseng extract, fragrant cananga hydrosol, deionized water, pentanediol, 1,3 butanediols and antibacterial composition.
Preferably, the above-mentioned all natural ingredients compositions delaying face skin aging, what wherein said each component was counted by weight consists of:
It is further preferred that the above-mentioned all natural ingredients compositions delaying face skin aging, what wherein said each component was counted by weight consists of:
Further preferably, delaying all natural ingredients compositions that face skin is old and feeble as mentioned above, it is facial cream, and described antibacterial is Radix Dauci Sativae antibacterial.
It addition, the preparation method of the above-mentioned all natural ingredients compositions facial cream delaying face skin aging provided by the present invention, it comprises the steps of:
(1) weigh the glabridin of recipe quantity, Rhizoma Curcumae Longae extract, Fructus Piperis extract, Radix Ginseng extract, dissolve with 1,3 butanediol respectively, standby;
(2) take A, B totally 2 beakers, A beaker adds the peony seed oil of recipe quantity, excellent olive emulsifing wax and MEIGUI CHUNLU;
(3) in B beaker, add the fragrant cananga hydrosol of recipe quantity, deionized water, vitamin B5And vitamin B3, stirring is to being completely dissolved;
(4) A beaker and B beaker are heated in a water bath, heat while stirring, until decorating film dissolves, temperature stops heating after reaching 80 DEG C, the solution in B beaker is poured in A beaker according to a small amount of mode repeatedly and quickly stirs;
(5) when the temperature of liquid to be mixed is down to 50 DEG C, it is sequentially added into the vitamin E of recipe quantity, little molecule squalane, biological polyoses glue, glabridin solution, Fructus Piperis extract solution, Rhizoma Curcumae Longae extract solution, Radix Ginseng extract solution, full effect extracellular polysaccharide and pentanediol, often adds a kind of component and stir 1-3 minute;
(6) it is eventually adding the antibacterial of recipe quantity, stirs.
It should be noted that, the preparation method delaying all natural ingredients compositions facial cream of face skin aging as mentioned above, excellent olive emulsifing wax therein is the excellent olive liquid crystal Fructus Canarii albi emulsifing wax of the celestial graceful production of France, English name OLDERMCRYSTALMOIST, INCI is sorbitol olive oil acid esters, the hard ester group glucoside of spermaceti;Described full effect extracellular polysaccharide imitates extracellular polysaccharide 4.0 entirely for France codif;Biological polyoses glue-2 rhamnose that described biological polyoses glue is the celestial graceful production of France is releived agent;Described Rhizoma Curcumae Longae extract is commercially available prod, and trade name is Sabiwhite, U.S. SABINSA produce, and purity is 95%Sabiwhite;Described Fructus Piperis extract is commercially available prod, and trade name is Cosmoperine, U.S. SABINSA produce, purity >=98% tetrahydrochysene piperine;Described fragrant cananga hydrosol is commercially available Madagascar's fragrant cananga hydrosol, and Cananga odorata quintessence oil content is 0.025%.
Preferably, the preparation method delaying all natural ingredients compositions facial cream of face skin aging as mentioned above, Radix Ginseng extract therein prepares as follows: using Radix Ginseng powder as raw material, butanediol and water are pressed after equal-volume mixes as Extraction solvent, room temperature lucifuge lixiviate, extraction time is 30-60d, filters, and filter vacuum concentrates and get final product.
Find according to antioxygenic property experimental study, contain the non-oxidizability of the sample 1 of peony seed oil and Radix Ginseng extract to be significantly higher than and only add the Radix Ginseng extract sample 2 without peony seed oil simultaneously, and be significantly higher than and only add peony seed oil without the sample 3 of Radix Ginseng extract, this illustrates peony seed oil and Radix Ginseng extract are used in combination according to a certain percentage has the collaborative effect removing skin histology excessive free radicals in skin care item.Therefore, present invention also offers the new opplication of two kinds of specific components couplings, it may be assumed that the application that peony seed oil is removed in skin histology in the skin care item of excessive free radicals in preparation with Radix Ginseng extract;Or peony seed oil and Radix Ginseng extract application in preparation non-oxidizability skin care item.It is further preferred that described skin care item are facial cream.
Compared with prior art, the pure natural compositions facial cream of the present invention has technique effect useful as follows:
(1) there is whitening, moisturizing, antioxidation, desalination mottle, reduce wrinkle, anti-inflammation, uvioresistant and maintenance face skin are persistently moistened and waits multi-efficiency, not only can effectively moisten face skin, and it is good with skin cell affinity, permeability is strong, very easily utilized by face percutaneous absorption, various nutrition required for face skin can be provided all sidedly, more can repair rapidly damaged cell, recover skin of face cytoactive, smooth out wrinkles, delay face old and feeble, keep the persistently pale colour of skin, allow face reappear at an early age delicate moisten, antagonism face skin is aging.
(2) face skin occurs under the serious water shortage state of dry decortication in the winter time; as long as applying the pure natural antioxidation whitening multiple-effect washing cream that last layer contains peony seed oil gently; and massaged gently; just can intimately merge with face skin, permeate rapidly skin and protecting film can be formed at face; moisture is allowed to stay veritably on skin; the coarse decortication occurred away from the reason lacked because of stimulation and internal water, the vitamin etc. of contact wind, dirt, ultraviolet and color make-up residual and the puzzlement that causes to skin of lost its bounce, make that face skin recovers elastic force, compact He Shuirun.
(3) compositions saves effective active composition well, the nutrient required for face skin can be supplemented, its major function is in that protection and repairs, for suppressing face melanin formation, delaying skin aging, moisturizing, minimizing wrinkle, compact delicacy and nutrition etc. can play good effect, accurately act on face skin cell, permeability is absorbed and used by force and very easily, rich in multiple effective ingredient, can effectively active cell metabolism, nourish skin, repair damaged cell, smooth years vestige, desalination mottle, and lasting stability effect can be kept.
(4) one layer of anti-oxidation protection film can be formed on face skin surface and can fully penetrate into skin depths imparting skin vigor;With the addition of and there is heigh antioxidation and alimentary peony seed oil and Radix Ginseng extract;With the addition of simultaneously and there is height moist type, humidity-preserving type and infiltrative natural small molecule squalane composition;Also added and there is the MEIGUI CHUNLU nourishing skin, activating cell and natural lock water effect.
(5) after smearing face skin a period of time, face presents the tender skin of pale water as baby, and can keep persistently moistening, tender skin highlight the whitening colour of skin.Natural black whitening material glabridin, Rhizoma Curcumae Longae extract and Fructus Piperis extract is taken off because which are added, can effectively suppress the activity of the key enzyme tryrosinase that dermal melanin synthesizes, thus stoping melanin to be formed, extremely strong with the affinity of skin, face skin can be played long-time moist tender white effect.
(6) compositions uses pure natural antibacterial composition, reduce and add skin stimulation and the allergic symptom that synthetic preservative brings, add the natural moisture preserving agent that pentanediol is excellent performance and there is anti-corrosion function, the filtrate that Radix Dauci Sativae antibacterial is Carrot Roots tunning added, there is very strong sterilization sterilization functions, it is all natural anticorrosion composition, skin is non-stimulated.
(7) to be that multiple component natural, green, safe is composite form compositions facial cream, have skin care moisturizing, Whitening, spot, antioxidation, nontoxic, resist the feature of ultraviolet etc., pure natural antioxidation whitening multiple-effect washing cream containing peony seed oil can be not only used for that face skin dries, the jaundice of pigmentation, skin, skin darkening, wrinkle are deepened and colonization when following the string, also can as preventing the anti-aging product that face skin is aging, the development trend of facial-care product will be become, there is wide market prospect and economic worth.
Detailed description of the invention
The following is specific embodiments of the invention, technical scheme is done to be described further, but protection scope of the present invention is not limited to these embodiments.Every change without departing substantially from present inventive concept or equivalent replacement are included within protection scope of the present invention.
The preparation of embodiment 1-3 facial cream
Formula is in Table 1:
The formula of table 1 facial cream compositions
It should be noted that the excellent olive liquid crystal Fructus Canarii albi emulsifing wax that excellent olive emulsifing wax is the celestial graceful production of France, English name OLDERMCRYSTALMOIST, INCI are sorbitol olive oil acid esters, the hard ester group glucoside of spermaceti;The organic MEIGUI CHUNLU of Bulgaria that described MEIGUI CHUNLU is commercially available, Flos Rosae Rugosae quintessence oil content is 0.05%;Described full effect extracellular polysaccharide imitates extracellular polysaccharide 4.0 entirely for France codif;Biological polyoses glue-2 rhamnose that described biological polyoses glue is the celestial graceful production of France is releived agent;Described Rhizoma Curcumae Longae extract is commercially available prod, and trade name is Sabiwhite, U.S. SABINSA produce, and purity is 95%Sabiwhite;Described Fructus Piperis extract is commercially available prod, and trade name is Cosmoperine, U.S. SABINSA produce, purity >=98% tetrahydrochysene piperine;Described fragrant cananga hydrosol is commercially available Madagascar's fragrant cananga hydrosol, and Cananga odorata quintessence oil content is 0.025%.
The preparing process of above-mentioned formula is as follows:
(1) preparation of Radix Ginseng extract
Take Radix Ginseng 15g → cleaning → pulverizing → 100ml butanediol+100ml distilled water lixiviate (extraction time is 45d, and front 7d often rocks and shakes up for solid-liquid ratio 3:20, room temperature lucifuge lixiviate) → filtering vacuum concentration → Radix Ginseng extract finished product.
(2) solution preparation
A, glabridin (90%Glabridin) solution preparation
Accurately weigh glabridin powder (production of SABINSA company of the U.S.) 0.3g, dissolve with the mass ratio of 1:9 with 1,3 butanediol, be configured to alcoholic solution standby;
B, Rhizoma Curcumae Longae extract solution preparation
Accurately weighing Rhizoma Curcumae Longae extract (SABINSA company of the U.S. produces, trade name SabiWhite, purity 95%) 0.3g, dissolve with the mass ratio of 1:8 with 1,3 butanediol, pH value controls, at 6.5-7, to be configured to alcoholic solution standby;
C, Fructus Piperis extract solution preparation
Accurately weigh Fructus Piperis extract (SABINSA company of the U.S. produces, trade name Cosmoperine, purity 95%) each 0.3g, dissolve with the mass ratio of 1:9 with 1,3 butanediol, be configured to alcoholic solution standby;
D, Radix Ginseng extract solution preparation
Accurately weigh Radix Ginseng extract 0.3g, dissolve with 1,3 butanediol of surplus, be configured to alcoholic solution standby.
(3) deionized water processing procedure:
1. impurities in water is filtered;
2. high pressure passes through reverse osmosis membrane;
3. ultraviolet-sterilization;
4. ion exchange process maximum resistance rate 18,000,000 is carried out.
(4) beaker taking A, B2 only clean is stand-by, adds the peony seed oil of recipe quantity, excellent olive emulsifing wax and MEIGUI CHUNLU in A beaker;
(5) adding the fragrant cananga hydrosol of recipe quantity, deionized water, vitamin B5 and vitamin B3 in B beaker, stirring is to being completely dissolved;
(6) beaker A and the beaker B heating that is placed on electromagnetic oven constant temperature water proof, heat while stirring, until decorating film dissolves, temperature is lifted down beaker A, B after reaching 80 DEG C immediately, water preparation in B beaker is poured in beaker A according to a small amount of method repeatedly and quickly stirs, approximate time about 30 minutes;
Etc. (7) when the temperature of mixture drops to 50 DEG C, add the vitamin E of recipe quantity, little molecule squalane, biological polyoses glue, glabridin, Fructus Piperis extract, Rhizoma Curcumae Longae extract, Radix Ginseng extract, full effect extracellular polysaccharide and pentanediol according to this, often add a kind of additive and stir 2 minutes;
(8) being eventually adding the Radix Dauci Sativae antibacterial of recipe quantity, the sterilization filling that stirs both may be used;
(9) facial cream that Packs sterilized installs seals and preserves under room temperature, 12 months effect duration.
The pure natural antioxidation whitening multiple-effect washing cream containing peony seed oil prepared by above-mentioned formula and technique is utilized to have following effect:
(1) full nutrition face skin, repair damaged cell, recover skin of face cytoactive, keeps the persistently pale colour of skin, allow face reappear at an early age delicate moisten, resist face skin aging;
(2) rapid infiltration face skin and can form protecting film, nourishes face skin, makes that face skin recovers elastic force, compact He Shuirun;
(3) effectively prevention stops that melanin is formed, and prevents pigementation;
(4) effectively facilitate cell regeneration, reduce wrinkle, delaying skin aging;
(5) allergy that effectively prevention face skin causes because of wind, dirt, color make-up etc.;
(6) injury effectively stoping ultraviolet that skin is caused, has sun-proof function.
Embodiment 4 facial cream moisture-absorbing moisture-keeping performance research
Embodiment 1-3, glycerol, hyaluronic acid are respectively taken 6 parts and is divided into 2 groups, respectively each sample is contained on the surface plate that diameter is 4cm, be precisely weighed after first group of sample is put into the exsiccator process 24h filling silica gel and measure for hydroscopicity under high humidity.Put into the vacuum desiccator that vacuum is-0.1MPa is precisely weighed after dried 24h by second group of sample equally and measure for hydroscopicity under low-moisture conditions.Under room temperature (25 DEG C), relative humidity (RH) is the exsiccator of the saturated ammonium sulfate aqueous solution of 81% is super-humid conditions, and relative humidity (RH) is the exsiccator of 43% is low humidity conditions.Sample accurately weighs each sample mass after the moisture absorption of 24h, by sample of poor quality before and after moisture absorption, obtains hydroscopicity according to following formula:
Hydroscopicity=(Mn-M0)/M0× 100% formula (1)
In formula (1): M0For quality before sample moisture absorption, Mn is quality after moisture absorption 24h.Continue to place 24h after the moisture absorbed reaches capacity, accurately weigh each sample mass, of poor quality by before and after sample, obtain moisturizing rate according to following formula:
Moisturizing rate=Hn/H0× 100% formula (2)
In formula (2): HnFor sample containing water quality, H0For sample after placing 24h containing water quality
Sample moisture-absorbing moisture-keeping type result of the test is in Table 2.
Table 2 sample moisture absorbability and moisture retentivity result of the test
From the test statistics result of table 2 it can be seen that high humidity the hygroscopicity of embodiment 1-3 do not highlight, but moistening effect is apparently higher than glycerol and hyaluronic acid;Under low humidity conditions, embodiment 1-3 moisture absorption, moisture retention are very prominent, are better than glycerol and hyaluronic acid, especially have good hygroscopicity and moisture-absorbing moisture-keeping effect stability.Hence for the weather that winter is relatively dry, embodiment facial cream can play good moistening effect, is desirable preserving moisture and protecting skin facial cream.
Embodiment 5 whitening performance test
Test arranges 3 samples, sample 1,2 is respectively embodiment 1 and embodiment 2 facial cream, sample 3 (positive controls) is vitamin C, it is configured to the aqueous solution of 50 μ g/L respectively, take 2ml test sample (blank sample replaces with phosphate buffer) and the mixed liquor of 1ml tryrosinase (100u), hatch 10min for 37 DEG C, add 0.003mol L-1Dopa reaction liquid 1ml, continues to hatch 2min at 37 DEG C, measures absorbance at 475nm place, and each sample repeats for 3 times, and inhibitory activity against tyrosinase is calculated as follows:
Suppression ratio={ [(A-B)-(C-D)]/(A-B) } × 100%
Wherein, A does not add sample and adds the mixed liquor of the tryrosinase absorbance at 475nm place;B does not add sample and does not add the mixed liquor of the tryrosinase absorbance at 475nm place;C is the mixed liquor adding sample and the tryrosinase absorbance at 475nm place;D adds sample not add the mixed liquor of the tryrosinase absorbance at 475nm place.Suppression ratio shows that more greatly whitening capability is more strong.Result of the test is in Table 3.
Table 3 sample whitening performance test results
Can be seen that from the test statistics result of table 3, embodiment 1 and embodiment 2 are when concentration is 50 μ g/L, to the suppression ratio of network tryrosinase respectively 47.86% and 43.90%, as can be seen here, embodiment 1 and embodiment 2 are respectively provided with stronger tyrosinase inhibitory action, and suppression ratio is above positive control VC, it is shown that whitening potentiality preferably.
Embodiment 6 antioxygenic property is tested
Test arranges 3 samples, sample 1 is embodiment 1 facial cream, sample 2 is replace peony seed oil (other components are with embodiment 1), sample 3 to replace Radix Ginseng extract (other components are with embodiment 1) for deionized water with oil-soluble VE, and the ethanol compound concentration with 95% is 0.04mg/mlDPPH solution.Pipetting 1ml sample solution and 3mlDPPH solution in tool plug test tube, fully after mixing, under room temperature, lucifuge measures absorbance A after standing 30min under 517nm1.Sample solution is replaced to make blank determination absorbance A with 1ml ethanol0, the absorbance A of DPPH measured in solution sample solution is replaced with 3ml ethanol2.Calculate the clearance rate (experimental result is in Table 4) of DPPH free radical according to the following formula:
The clearance rate of DPPH free radical=(1-(A1-A2)/A0) × 100%
Table 4 each sample clearance rate to DPPH free radical
DPPH is a kind of very stable free radical, this be due to the Resonance Stabilization action of its phenyl ring and spatial obstacle effect to make to be on the nitrogen-atoms of central authorities lonely to electronics can not be paired.DPPH is dark purple in alcoholic solution, when there being free radical scavenger to exist, in DPPH, the lone pair electrons on nitrogen are paired and solution colour are shoaled, absorbance at maximum absorption wavelength 517nm place diminishes, therefore absorbance is more little, clearance rate is more big, it was shown that the oxidation resistance of scavenger is more strong.Can be seen that from the test statistics result of table 4, the clearance rate to DPPH free radical of sample 1 solution and sample 2, sample 3 significant difference, the clearance rate of sample 1 is up to 83.19%, show stronger oxidation resistance, sample 2 and sample 3 are notable to the clearance rate difference of DPPH free radical, and it is to the clearance rate of DPPH free radical respectively 32.65% and 45.56%.Component from 3 samples, sample 1 is containing peony seed oil and Radix Ginseng extract, sample 2 is without peony seed oil, all the other components are with sample 1, sample 3 is without Radix Ginseng extract, all the other components are with sample 1, it is not difficult to find out to the clearance rate of DPPH free radical, contain the non-oxidizability of the sample 1 of peony seed oil and Radix Ginseng extract to be significantly higher than and only add Radix Ginseng extract and without the sample 2 of peony seed oil and only add the peony seed oil sample 3 without Radix Ginseng extract simultaneously, and the non-oxidizability of sample 1 is noticeably greater than sample 2 and sample 3, this illustrates peony seed oil and Radix Ginseng extract are used in combination according to a certain percentage has the collaborative effect removing skin histology excessive free radicals in skin care item.

Claims (10)

1. delaying all natural ingredients compositions that face skin is old and feeble, it is by peony seed oil, little molecule squalane, excellent olive emulsifing wax, MEIGUI CHUNLU, vitamin E, vitamin B5, vitamin B3, biological polyoses glue, entirely imitate extracellular polysaccharide, glabridin, Rhizoma Curcumae Longae extract, Fructus Piperis extract, Radix Ginseng extract, fragrant cananga hydrosol, deionized water, pentanediol, 1,3 butanediols and antibacterial composition.
2. delay all natural ingredients compositions that face skin is old and feeble according to claim 1, it is characterised in that what described each component was counted by weight consists of:
3. delay all natural ingredients compositions that face skin is old and feeble according to claim 2, it is characterised in that what described each component was counted by weight consists of:
4. according to claim 1 or 2 or 3, delay all natural ingredients compositions that face skin is old and feeble, it is characterised in that described antibacterial is Radix Dauci Sativae antibacterial.
5. according to Claims 2 or 3, delay all natural ingredients compositions that face skin is old and feeble, it is characterised in that described compositions is facial cream.
6. the preparation method of all natural ingredients compositions facial cream delaying face skin aging according to claim 5, it is characterised in that the method comprises the steps of:
(1) weigh the glabridin of recipe quantity, Rhizoma Curcumae Longae extract, Fructus Piperis extract, Radix Ginseng extract, dissolve with 1,3 butanediol respectively, standby;
(2) take A, B, C totally 3 beakers, A beaker adds the peony seed oil of recipe quantity, excellent olive emulsifing wax and MEIGUI CHUNLU;
(3) in B beaker, add the fragrant cananga hydrosol of recipe quantity, deionized water, vitamin B5And vitamin B3, stirring is to being completely dissolved;
(4) A beaker and B beaker are heated in a water bath, heat while stirring, until decorating film dissolves, temperature stops heating after reaching 80 DEG C, the solution in B beaker is poured in A beaker according to a small amount of mode repeatedly and quickly stirs;
(5) when the temperature of liquid to be mixed is down to 50 DEG C, it is sequentially added into the vitamin E of recipe quantity, little molecule squalane, biological polyoses glue, glabridin solution, Fructus Piperis extract solution, Rhizoma Curcumae Longae extract solution, Radix Ginseng extract solution, full effect extracellular polysaccharide and pentanediol, often adds a kind of component and stir 1-3 minute;
(6) it is eventually adding the antibacterial of recipe quantity, stirs.
7. the preparation method delaying all natural ingredients compositions facial cream of face skin aging according to claim 6, it is characterized in that, described Radix Ginseng extract prepares as follows: using Radix Ginseng powder as raw material, butanediol and water are pressed after equal-volume mixes as Extraction solvent, room temperature lucifuge lixiviate, extraction time is 30-60d, filters, and filter vacuum concentrates and get final product.
8. the application that peony seed oil is removed in skin histology in the skin care item of excessive free radicals in preparation with Radix Ginseng extract.
9. peony seed oil and the Radix Ginseng extract application in preparation non-oxidizability skin care item.
10. application according to claim 8 or claim 9, it is characterised in that described skin care item are facial cream.
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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108309828A (en) * 2018-03-19 2018-07-24 恒天量子(厦门)科技有限公司 One kind heal-care essential oil containing peony seed oil and preparation method thereof, cosmetics
CN111297761A (en) * 2020-04-13 2020-06-19 北京悠悦百仕科技发展有限公司 Whitening composition and preparation method and application thereof
CN113413353A (en) * 2021-08-12 2021-09-21 青海湖宝生物科技有限公司 Plant extract essence milk containing plateau sea buckthorn and preparation method thereof
CN115887246A (en) * 2022-12-01 2023-04-04 美出莱(杭州)化妆品有限责任公司 Novel microcapsule composition with multiple-effect anti-aging effect and preparation method and application thereof
CN115887246B (en) * 2022-12-01 2023-09-15 美出莱(杭州)化妆品有限责任公司 Novel microcapsule composition with multiple effects of resisting aging and preparation method and application thereof

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