Whitening composition and preparation method and application thereof
Technical Field
The invention belongs to the field of daily chemicals, and particularly relates to a whitening composition and a preparation method and application thereof.
Background
The safety and efficacy of cosmetics are issues of intense interest to consumers and skin care product developers. At present, a plurality of whitening methods exist, and if the skin problems are rapidly and effectively improved in a short time and the face appearance is improved, scientific beautifying and skin care products are needed.
Most of the whitening products on the market take nicotinamide and VC as main whitening agents. Niacinamide is a water-soluble vitamin and is a member of the vitamin B group. Nicotinamide can inhibit the transfer of melanin, and make skin slowly whiten and more bright. The high-content nicotinamide product has a certain sensitization rate. Meanwhile, melanin produced by the skin has a protective effect on the skin, and excessive inhibition of the production or formation of melanin may have an adverse effect on the skin. For example, the "chalking event by creosol" in Japan is the chalking phenomenon caused by severe destruction of skin melanin after the skin care agent is used. In addition, the other main whitening component VC is easy to discolor and unstable.
The realization of low whitening efficiency through a single approach is one of the problems of the conventional whitening products. Therefore, the development of safe and efficient skin whitening compositions is a problem to be solved in the art.
Disclosure of Invention
The skin-care product takes the nicotinamide as a main whitening agent, is compounded with the plant components of the giant knotweed extract and the pepper extract, and synergistically achieves the skin-care effects of removing free radicals, improving red blood streak, balancing skin color and balancing grease. According to the invention, the plant components such as the giant knotweed extract and the pepper extract are compounded with the nicotinamide, so that the problem of sensitization risk caused by large whitening amount of the nicotinamide is solved, the product safety is improved, the components are synergistic, and the problems of single whitening way and unobvious effect are solved.
In order to solve the problems, the invention adopts the following technical scheme:
the invention provides a whitening composition, which comprises the following raw materials in parts by weight: 1-10 parts of nicotinamide, 0.01-5 parts of giant knotweed extract and 0.01-0.2 part of pepper extract.
According to the invention, the nicotinamide is used in an amount of 1 to 10 parts by weight, and may be, for example, 1, 1.1, 1.5, 2, 2.2, 2.3, 2.4, 2.5, 2.8, 3, 3.1, 3.2, 3.5, 3.8, 4, 4.5, 5, 5.5, 5.8, 6, 6.4, 6.5, 7, 7.4, 7.6, 7.8, 8, 8.8, 9, 9.5, 10 parts by weight, and also values between the aforementioned values.
According to the invention, the polygonum cuspidatum extract is used in an amount of 0.01-5 parts by weight, such as 0.01, 0.02, 0.03, 0.05, 0.1, 0.2, 0.3, 0.6, 0.8, 0.9, 1, 1.1, 1.5, 2, 2.2, 2.3, 2.4, 2.5, 2.8, 3, 3.1, 3.2, 3.5, 3.8, 4, 4.5, 5 parts by weight, and values in between.
According to the invention, the pepper extract is used in an amount of 0.01-0.2 parts by weight, which may be, for example, 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.08, 0.09, 0.1, 0.15, 0.2 parts by weight, and values in between.
According to some embodiments of the invention, the composition comprises the following raw materials in parts by weight: 2-6 parts of nicotinamide, 0.1-4 parts of giant knotweed extract and 0.1-0.2 part of pepper extract.
According to some embodiments of the invention, the composition further comprises 1, 3-propanediol.
According to some embodiments of the invention, the composition further comprises PEG-40 hydrogenated castor oil.
According to some further embodiments of the present invention, the composition comprises the following raw materials in parts by weight: 1-10 parts of nicotinamide, 0.01-5 parts of giant knotweed extract, 0.01-0.2 part of pepper extract, 0.01-5 parts of 1, 3-propylene glycol and 0.02-0.5 part of PEG-40 hydrogenated castor oil.
According to the invention, the 1, 3-propanediol is used in an amount of 0.01 to 5 parts by weight, and may be, for example, 0.01, 0.05, 0.1, 0.2, 0.3, 0.5, 0.6, 0.8, 0.9, 1, 1.1, 1.5, 1.8, 2, 2.2, 2.3, 2.4, 2.5, 2.8, 3, 3.1, 3.2, 3.5, 3.8, 4, 4.5, 5 parts by weight, and also values in between the above-mentioned values.
According to the invention, the PEG-40 hydrogenated castor oil is used in an amount of 0.02 to 0.5 parts by weight, and may be, for example, 0.02, 0.03, 0.05, 0.06, 0.1, 0.15, 0.2, 0.25, 0.3, 0.33, 0.35, 0.4, 0.45, 0.47, 0.48, 0.5 parts by weight, and points between the above values.
According to some preferred embodiments of the present invention, the composition comprises 2-6 parts by weight of nicotinamide, 0.1-4 parts by weight of giant knotweed rhizome extract, 0.1-0.2 part by weight of pepper extract, 0.25-5 parts by weight of 1, 3-propanediol and 0.2-0.4 part by weight of PEG-40 hydrogenated castor oil.
In a second aspect of the present invention, there is provided a method for preparing the whitening composition of the first aspect.
According to some embodiments of the invention, the method of preparing comprises the steps of:
(1) mixing fructus Piperis extract with 1, 3-propylene glycol and PEG-40 hydrogenated castor oil, and dissolving to obtain mixed solution;
(2) adding nicotinamide and giant knotweed rhizome extract into the mixed solution obtained in the step (1), and uniformly stirring.
According to the present invention, the whitening composition is prepared at normal temperature.
In a third aspect of the present invention, there is provided a whitening composition according to the first or second aspect of the present invention for use in a skin care product.
According to the invention, the whitening composition can be applied to various formulations of skin care products such as cream, emulsion, facial mask, gel, water aqua, eye cream and the like, and a proper amount of the whitening composition can be added according to the formula and efficacy requirements.
According to the present invention, the suggested addition amount of the whitening composition in the skin care product is 0.5 wt% to 20wt%, preferably 3 wt% to 10wt%
According to some embodiments of the invention, the skin care product is a cream.
According to some embodiments of the invention, the cream comprises the following raw materials in parts by weight:
according to the invention, the cream can be prepared by a method comprising the following steps:
step 1: adding water, xanthan gum, dipropylene glycol, glycerol, carbomer and EDTA disodium into an emulsification tank, heating to 80-85 deg.C, mixing and stirring;
step 2: adding arachidyl alcohol/behenyl alcohol/arachidyl alcohol glucoside, glycerol tri (ethyl hexanoate), pentaerythritol distearate, and cyclopenta dimethyl silicone into an oil phase tank, heating to 80-85 deg.C, mixing, and stirring;
and step 3: pumping the oil phase in the oil phase tank into an emulsification tank, starting homogenization at the rotation speed of 2500-.
And 4, step 4: cooling to 45-60 deg.C, and adding sodium hydroxide;
and 5: adding the composition of the invention and stirring uniformly.
According to the skin care composition, the skin care composition is formed by compounding the nicotinamide with the plant source polygonum cuspidatum extract and the plant source pepper extract, so that the problems of poor whitening effect and safety possibly caused by the independent use of the nicotinamide are solved, the effects of removing free radicals, removing red blood streaks and controlling oil can be achieved while whitening through the compounding and synergistic effect of various components, and the skin care composition has a wide application prospect in skin care products.
Description of the drawings:
FIG. 1 is a graph showing the radical scavenging effect of example 4 and comparative examples 1 to 5;
FIG. 2 is a chart of the results of the VISIA test of skin color after the subject uses the sample of example 5;
FIG. 3 is a chart of the results of the VISIA test of red blood streak status after a subject has tried a sample of example 5;
FIG. 4 is a chart of the results of the VISIA test of skin color after the subject uses the sample of example 6;
FIG. 5 is a chart of the results of the VISIA test of red blood streak status after the subject has tried the sample of example 6;
FIG. 6 is a chart of the results of the VISIA test of skin color after the subject uses the sample of example 7;
FIG. 7 is a chart of the results of the VISIA test of red blood streak status after a subject has tried a sample from example 7;
FIG. 8 is a chart of the results of the VISIA test of skin color after the subject uses the sample of example 8;
FIG. 9 is a chart of the results of the VISIA test of red blood streak status after a subject has tried a sample from example 8;
FIG. 10 is a chart of the results of the VISIA test of skin color after the subject tried the comparative example 6 sample;
FIG. 11 is a chart showing the results of the VISIA test on red blood streak following the trial of comparative example 6 by a subject;
FIG. 12 is a chart of the results of the VISIA test of skin color after the subject uses the comparative example 8 sample;
FIG. 13 is a chart of the VISIA test results of red blood streak following the trial of comparative example 8 by a subject;
FIG. 14 is a chart of the results of the VISIA test of skin color after the subject uses the comparative example 9 sample;
FIG. 15 is a chart of the VISIA test results of red blood streak following the trial of comparative example 9 by a subject;
FIG. 16 is a chart of the results of the VISIA test of skin color after the subject uses the comparative example 10 sample;
FIG. 17 is a chart of the VISIA test results of red blood streak following the trial of comparative example 10 by a subject;
FIG. 18 is a chart showing the results of the VISIA test on oil and fat secretion by a subject after sampling the sample of example 8;
FIG. 19 is a VISIA test result chart of the oil secretion of the blank control sample after the test of the subject.
The specific implementation mode is as follows:
the present invention will be described in detail with reference to the following embodiments, which will become more apparent by describing the embodiments and processes of the present invention. It should be understood by those skilled in the art that the detailed description is only a part of the examples, but not all examples, and is intended to illustrate the invention and not to limit the scope of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention. The examples were carried out under the conventional conditions, unless otherwise specified. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.
Table main raw material and source
Example 1
Weighing the following raw materials in parts by weight:
1 part by weight of nicotinamide, 5 parts by weight of giant knotweed extract, 0.01 part by weight of pepper extract, 0.01 part by weight of 1, 3-propylene glycol and 0.02 part by weight of PEG-40 hydrogenated castor oil;
mixing the above fructus Piperis extract with 1, 3-propylene glycol and PEG-40 hydrogenated castor oil, and dissolving to obtain mixed solution;
adding nicotinamide and giant knotweed rhizome extract into the mixed solution, and uniformly stirring to obtain the whitening composition A.
Example 2
Weighing the following raw materials in parts by weight:
10 parts of nicotinamide, 0.01 part of giant knotweed rhizome extract, 0.2 part of pepper extract, 5 parts of 1, 3-propylene glycol and 0.4 part of PEG-40 hydrogenated castor oil.
Mixing the above fructus Piperis extract with 1, 3-propylene glycol and PEG-40 hydrogenated castor oil, and dissolving to obtain mixed solution;
and adding the nicotinamide and the polygonum cuspidatum extract into the obtained mixed solution, and uniformly stirring to obtain the whitening composition B.
Example 3
Weighing the following raw materials in parts by weight:
3 parts of nicotinamide, 3 parts of giant knotweed rhizome extract, 0.1 part of pepper extract, 2.5 parts of 1, 3-propylene glycol and 0.2 part of PEG-40 hydrogenated castor oil,
mixing the above fructus Piperis extract with 1, 3-propylene glycol and PEG-40 hydrogenated castor oil, and dissolving to obtain mixed solution;
adding nicotinamide and giant knotweed rhizome extract into the mixed solution, and uniformly stirring to obtain the whitening composition C.
Example 4
Weighing the following raw materials in parts by weight:
6 parts of nicotinamide, 5 parts of giant knotweed rhizome extract, 0.2 part of pepper extract, 5 parts of 1, 3-propylene glycol, 0.4 part of PEG-40 hydrogenated castor oil,
mixing the above fructus Piperis extract with 1, 3-propylene glycol and PEG-40 hydrogenated castor oil, and dissolving to obtain mixed solution;
and adding the nicotinamide and the polygonum cuspidatum extract into the obtained mixed solution, and uniformly stirring to obtain the whitening composition D.
Comparative example 1
Weighing the following raw materials in parts by weight:
2 parts of nicotinamide, 9 parts of giant knotweed rhizome extract, 0.2 part of pepper extract, 5 parts of 1, 3-propylene glycol, 0.4 part of PEG-40 hydrogenated castor oil,
mixing the above fructus Piperis extract with 1, 3-propylene glycol and PEG-40 hydrogenated castor oil, and dissolving to obtain mixed solution;
adding nicotinamide and rhizoma Polygoni Cuspidati extract into the mixed solution, and stirring to obtain skin care composition.
Comparative example 2
Weighing the following raw materials in parts by weight:
3 parts of nicotinamide, 8 parts of giant knotweed rhizome extract, 0.2 part of pepper extract, 5 parts of 1, 3-propylene glycol, 0.4 part of PEG-40 hydrogenated castor oil,
mixing the above fructus Piperis extract with 1, 3-propylene glycol and PEG-40 hydrogenated castor oil, and dissolving to obtain mixed solution;
adding nicotinamide and rhizoma Polygoni Cuspidati extract into the mixed solution, and stirring to obtain skin care composition.
Comparative example 3
Weighing the following raw materials in parts by weight:
6 parts of nicotinamide, 5 parts of pseudo-ginseng extract, 0.2 part of pepper extract, 5 parts of 1, 3-propylene glycol, 0.4 part of PEG-40 hydrogenated castor oil,
mixing the above fructus Piperis extract with 1, 3-propylene glycol and PEG-40 hydrogenated castor oil, and dissolving to obtain mixed solution;
adding nicotinamide and Notoginseng radix extract into the mixed solution, and stirring to obtain skin care composition.
Comparative example 4
Weighing the following raw materials in parts by weight:
6.0 parts of nicotinamide, 5.2 parts of giant knotweed extract, 5 parts of 1, 3-propylene glycol, 0.4 part of PEG-40 hydrogenated castor oil,
mixing the above fructus Piperis extract with 1, 3-propylene glycol and PEG-40 hydrogenated castor oil, and dissolving to obtain mixed solution;
adding nicotinamide and rhizoma Polygoni Cuspidati extract into the mixed solution, and stirring to obtain skin care composition.
Comparative example 5
Weighing the following raw materials in parts by weight:
VC ethyl ether 0.2 weight parts, giant knotweed rhizome extract 8.2 weight parts, pepper extract 0.3 weight parts, 1, 3-propylene glycol 7.5 weight parts, PEG-40 hydrogenated castor oil 0.6 weight parts,
mixing the above fructus Piperis extract with 1, 3-propylene glycol and PEG-40 hydrogenated castor oil, and dissolving to obtain mixed solution;
adding VC ethyl ether and rhizoma Polygoni Cuspidati extract into the mixed solution, and stirring to obtain skin care composition.
Efficacy evaluation test
Experiment for eliminating free radicals in vitro
1. Experimental methods
(1) DPPH free radical (DPPH) scavenging experiments
DPPH is a very stable nitrogen-centered radical, is very stable in organic solvents, is purple in color, has a characteristic absorption peak at 517nm, and when it encounters a radical scavenger, the lone electron of DPPH is paired, resulting in a lighter color and a lower absorbance at the maximum absorption wavelength. Therefore, the effect of removing DPPH can be evaluated by measuring the change in absorbance.
20mg of DPPH (Sigma Co.) was weighed out accurately, and the volume was adjusted to a 250ml volumetric flask using absolute ethanol to obtain a 20mmol/L DPPH solution, and the compositions prepared in example 4 and comparative example were diluted with distilled water to obtain test solutions having different concentrations, respectively. Respectively taking 2ml of test solution with different concentrations and 2ml of 20mmol/L DPPH, uniformly mixing, reacting for 30min, measuring the change of absorbance at the wavelength of 517nm, replacing a contrast solvent with absolute ethyl alcohol, and calculating the inhibition rate according to the following formula.
Inhibition (%) = [1- (Ai-Aj)/Ac ] × 100.
In the formula, Ai is the absorbance of a mixed solution consisting of 2ml of DPPH solution and 2ml of test solutions with different concentrations; aj is the absorbance of a mixed solution consisting of 2ml of test solutions with different concentrations and 2ml of absolute ethyl alcohol; ac was the absorbance of a mixture of 2ml of DPPH solution and 2ml of absolute ethanol.
(2) Hydroxy radical (. OH) scavenging experiments
The hydroxyl free radical is the most active free radical in active oxygen, can almost react with any biomacromolecule in living cells, has extremely high reaction speed, and is the free radical which has the greatest harm to organisms. The clearance rate of the hydroxyl radical is determined by measuring the product obtained by salicylic acid capturing the hydroxyl radical, and if a substance with the function of clearing the hydroxyl radical is added into the reaction system, the absorbance value of the reaction liquid can be reduced. The amount of hydroxyl radicals and the ability of the substance to be determined to scavenge hydroxyl radicals can therefore be described spectrophotometrically. The specific experimental process is as follows:
the compositions prepared in the above examples and comparative examples were diluted 50-fold. Hydroxyl radicals are generated by the Fenton reaction, OH oxidizes salicylic acid to generate 2, 3-dihydroxybenzoic acid with characteristic absorption at 510nm, and the OH clearance is determined by measuring the product obtained by capturing OH with salicylic acid. 3ml of 2mmol/L FeSO4 and 3ml of 1mmol/LH2O2 are added into a 25ml colorimetric tube, the mixture is shaken up, then 3ml of 6mmol/L salicylic acid is added, the mixture is shaken up, the mixture is taken out after being heated in a water bath at 37 ℃ for 15min, and the absorbance A0 is measured. Then adding the above composition with concentration of 2% 0.2ml, 0.4ml, 0.6ml, 0.8ml and 1.0ml respectively, then adding distilled water 0.8ml, 0.6ml, 0.4ml, 0.2ml and 0ml respectively, shaking, heating in water bath for 15min, taking out and measuring absorbance Ax. In order to eliminate the decrease of absorbance value of the system caused by adding 1.0ml of the composition and distilled water, the same method is adopted, the absorbance value A00 is measured after keeping the temperature for 15min, then 1ml of distilled water is added, the absorbance Axx is measured after shaking up, and the decrease of A is A00-Axx.
The composition has the following clearance rate to OH: OH clearance (%) =100(a 0-Ax-a decrease)/a 0.
(3) Superoxide anion radical scavenging experiment
Taking 4.5ml of 0.05mol/L Tris-HCl buffer solution with pH 8.2, placing the solution in a water bath at 25 ℃ for preheating for 20min, respectively adding 1ml of the compositions prepared in the above examples and comparative examples and 0.4ml of 25mmol/L pyrogallol solution, uniformly mixing, reacting in the water bath at 25 ℃ for 5min, adding 1.0ml of 8mol/L HCl to terminate the reaction, taking the Tris-HCl buffer solution as a reference, measuring absorbance at 299nm, and calculating the clearance rate. Blank controls replaced the sample with 1ml of deionized water and three replicates for each treatment.
Formula for calculating clearance: superoxide anion radical clearance (%) =100(a1-a2)/a1, wherein a1 is the average absorbance of the blank and a2 is the average absorbance of the whitening composition.
2. The results of the experiment are shown in FIG. 1.
The results shown in the figure demonstrate that the examples of the present invention have a certain scavenging capacity for DPPH, hydroxyl radicals and superoxide anion radicals. The inventors repeated the above experiments with other examples to arrive at the same conclusion.
Example 5 preparation of skin cream
Raw materials and proportion:
the preparation method comprises the following steps:
step 1: adding water, xanthan gum, dipropylene glycol, glycerol, carbomer and EDTA disodium into an emulsification tank, heating to 80-85 ℃, and mixing and stirring uniformly;
step 2: adding arachidyl alcohol/behenyl alcohol/arachidyl alcohol glucoside, glycerol tri (ethyl hexanoate), pentaerythritol distearate, and cyclopenta dimethyl silicone into an oil phase tank, heating to 80-85 deg.C, mixing, and stirring;
and step 3: pumping the oil phase in the oil phase tank into an emulsification tank, starting homogenization at the rotation speed of 2500-.
And 4, step 4: cooling to 45-60 ℃, and adding sodium hydroxide for neutralization;
and 5: adding the whitening composition, stirring, and discharging.
Example 6 skin cream preparation
Example 6 cream composition a skin care composition was prepared using example 2, the rest being the same as in example 5.
Example 7 skin cream preparation
Example 7 cream composition a skin care composition was prepared using example 3, the rest being the same as in example 5.
Example 8 skin cream preparation
Example 8 cream composition a skin care composition was prepared using example 4, the rest being the same as in example 5.
Comparative example 6 preparation of skin cream
Comparative example 6 cream composition a skin care composition was prepared using comparative example 1, the rest being the same as in example 5.
Comparative example 7 skin cream preparation
Comparative example 7 cream composition a skin care composition was prepared using comparative example 2, the rest being the same as in example 5.
Comparative example 8 skin cream preparation
Comparative example 8 cream composition a skin care composition was prepared using comparative example 3, the rest being the same as in example 5.
Comparative example 9 preparation of skin cream
Comparative example 9 cream composition a skin care composition was prepared using comparative example 4, the rest being the same as in example 5.
Comparative example 10 skin cream preparation
Comparative example 10 cream composition a skin care composition was prepared using comparative example 5, the rest being the same as in example 5.
Evaluation experiment:
first, patch test
30 eligible subjects were selected for testing and informed consent was filled out.
Selecting qualified spot test materials. Putting the tested object into a spot tester, wherein the dosage is about 0.020 g to 0.025 g; where # 0 is a blank control and no sample is present in the plaque tester.
The spot test device with the tested object is applied to the back or the curved side of the forearm of the tested object by using a non-irritating adhesive tape, and is lightly pressed by the palm to be uniformly applied to the skin for 24 hours. And (5) removing the tested substance spot tester for 30min, and observing skin reaction after the indentation disappears. If the result is negative, the result is observed once more after 24 h and 48 h of the patch test respectively, and the reflection result is recorded.
0.5 hour observation of the surface despeckle tester
24-hour observation result of surface despeckle tester
According to the result of the spot sticking test, after 0.5 hour of a spot removal tester, 1 case of 1-grade adverse reaction appears in the No. 2 group, 2 cases of 1-grade adverse reaction appears in the No. 3 group, 2 cases of 1-grade adverse reaction appears in the No. 4 group, 1 case of 1-grade adverse reaction appears in the No. 5 group, 1 case of 1-grade adverse reaction appears in the No. 7 group, 1 case of 1-grade adverse reaction appears in the No. 8 group, and 4 cases of 1-grade adverse reaction appears in the No. 9 group; the adverse reactions of each group disappeared after 24 hours of removal of the plaque tester.
According to the results, the cream sample is evaluated and tested to have no adverse reaction on human skin.
Second, red melanin, skin color, red blood streak and oil secretion test
The test is carried out by adopting a single-center, random, double-blind and self-face left-right contrast method, and 68 subjects with dark and yellow skin color are screened for the test. The subject needs to use different tested whitening creams on the left face and the right face respectively in the morning and at the evening within 6 weeks, and the dosage is based on the actual dosage required for applying the whitening creams on the face. VISIA-CR, facial image capture, and MX18 instrumental measurements were performed 3 times before and at weeks 2 and 6 after the start of the test. Before each test, a subject needs to clean the face with clear water and then sits in a room with constant temperature and humidity for 30min, the face cannot be wiped with paper, and the test is carried out after the face is naturally dried; analyzing the improvement condition of the facial erythema and melanin of the subjects through the measurement result of the MX18 instrument; and analyzing the complexion condition and the improvement condition of the red blood streak before and after the test of the whitening cream by using a VISIA-CR skin test result graph.
1. Melanin content MI value
The experimental method comprises the following steps: the melanin content MI values were measured 3 times in the cheek regions of the subjects at weeks 0, 2 and 6, respectively, and averaged.
The apparatus used was: mexameter MX18
And (3) testing period: 6 weeks
The test results are given in the following table:
3. EI value of red pigment content
The experimental method comprises the following steps: at weeks 0, 2 and 6, 3 measurements of the EI value of the red pigment content were made in the cheek regions of the subjects, and averaged.
The apparatus used was: mexameter MX18
The test results are given in the following table:
the results show that the embodiment of the invention has improved content of skin melanin and haematochrome, and particularly, the embodiment 8 has more obvious improvement on the content of skin melanin and haematochrome; comparative example 7 did not significantly improve red and melanin.
Skin tone and redness testing
Experimental methods
VISIA-CR was used to observe the skin color and red blood streak improvement in subjects at 0 week 2 week 6 week.
The apparatus used was: VISIA-CR skin tester
Introduction of corresponding light sources:
skin color condition: standard Natural light 2
Red blood streak: red Areas Red region
And (3) testing period: 6 weeks
Results of the experiment
The experimental results of examples 5-8, comparative example 6, and comparative example 8-10 VISIA are shown in the attached figures 2-17.
As shown in the attached figures 2-17, the cream of examples 5-8 has certain effect on improving skin color and red blood streak after 6 weeks.
The VISIA picture results show that the examples of the invention have certain improvement effect on the skin color condition and the red blood streak of the subjects, wherein the effect of the example 8 is better, and the overall improvement effect of the comparative example is not good enough.
Third, oil secretion test
In addition, the inventor also unexpectedly finds that the cream has a certain inhibiting effect on skin oil secretion, so that 30 subjects with relatively strong skin oil secretion are screened out and divided into 2 groups, and 15 persons in each group; the test samples were used face-wide using a single-center, randomized, double-blind, placebo-controlled approach. One group used the example 8 sample and the other group used a blank sample (same as example 8 except that the whitening composition of the present invention was not added). The medicine is used once in the morning and at night every day, and the dosage is based on the actual dosage required for applying the medicine on the face. VISIA-CR was taken 3 times before the start of the test and at weeks 2 and 6 after the start of the test. Before each test, a subject needs to clean the face with clear water, sits still in a room with constant temperature and humidity for 30min, cannot wipe the face with paper, and shoots the face with a VISIA-CR skin tester after naturally drying; the oil secretion of the subjects before and after the whitening cream was applied was analyzed by a VISIA-CR skin test result chart.
The apparatus used was: VISIA-CR skin tester
Introduction of corresponding light sources:
oil secretion: porphyrin light
And (3) testing period: 6 weeks
Results of the experiment
Example 8, blank control sample VISIA experimental results are shown in fig. 18 and 19.
As shown in FIGS. 18 and 19, the cream of example 8 was used for 6 weeks, and then it was found to be effective in improving the secretion of skin oil.
As can be seen from the results of VISIA pictures, example 8 of the present invention has a certain effect of improving the secretion of oil in the subject, whereas comparative example 11 has a poor effect of improving the secretion of oil.
In conclusion, the whitening composition has the advantages that the ingredients are synergistic, the whitening effect is good, the effects of controlling oil, removing red blood streaks and improving skin color are good, and the whitening composition has a good application value in the field of skin care products.