CN105906731A - Production method and use of natural tremella polysaccharide - Google Patents
Production method and use of natural tremella polysaccharide Download PDFInfo
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- CN105906731A CN105906731A CN201610259099.6A CN201610259099A CN105906731A CN 105906731 A CN105906731 A CN 105906731A CN 201610259099 A CN201610259099 A CN 201610259099A CN 105906731 A CN105906731 A CN 105906731A
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
- C08B37/0087—Glucomannans or galactomannans; Tara or tara gum, i.e. D-mannose and D-galactose units, e.g. from Cesalpinia spinosa; Tamarind gum, i.e. D-galactose, D-glucose and D-xylose units, e.g. from Tamarindus indica; Gum Arabic, i.e. L-arabinose, L-rhamnose, D-galactose and D-glucuronic acid units, e.g. from Acacia Senegal or Acacia Seyal; Derivatives thereof
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Abstract
The invention discloses a production method of natural tremella polysaccharide, wherein the production method comprises the following steps: selecting dry pest-free and non-toxic tremella, and crushing for standby application; adding distilled water into a container containing the dry tremella, extracting, adjusting the pH to 9.0 or more with an alkali, and extracting for 24-28 h at room temperature; after completion of the extraction, adding cation resin, washing, and adjusting the pH to 7.0 or less; next, intercepting high-molecular-weight impurities with an ultrafiltration film; concentrating the filtrate with a single-effect concentrator to the proportion of 1:3-1:5; carrying out alcohol precipitation of the concentrated liquid with ethyl alcohol, stirring for 1-2 h, and allowing to stand for 12-18 h; then using a plate type filter press, and collecting a white solid, namely the tremella polysaccharide. The invention also discloses an application of the natural tremella polysaccharide in production of cosmetic raw materials. The natural tremella polysaccharide has excellent antioxidant property; compared with ordinary tremella polysaccharide, although the final concentrations of the tremella polysaccharides are the same, the natural tremella polysaccharide has more excellent antioxidant property and has the effect of preventing skin aging.
Description
Technical field
The present invention relates to the production method of a kind of natural silver fungus polysaccharides, the invention still further relates to the application in cosmetic material produces of a kind of natural silver fungus polysaccharides.
Background technology
White fungus, for the fructification of door Basidiomycota fungi white fungus, white fungus is made up of the pancake limb of more than the 10 thin and many gauffers of sheet.Tremella fructification is pure white to milky, is typically chrysanthemum shape or cockscomb shape, diameter 5～10 centimetres, soft pure white, translucent, and high resilience is made up of several pieces, likeness in form cosmos, tree peony shape or silk ball shape, diameter 3～15 centimetres.Dry after-contraction, cutin is hard and crisp, white or ecru.The raw limb surface of hymenium.Load is subsphaeroidal or nearly oval, vertical separates, and is born in summer and autumn on broad leaf tree corruption wood.
Wild white fungus rare numbers, belongs to famous and precious tonic in ancient times.White fungus is a kind of acid heteroglycan by obtaining tremella polysaccharides, its backbone structure is the mannosan connected by α-(1 → 3) glycosidic bond, side chain is made up of glucuronic acid and wood sugar, and its activated centre is this common structure part of α-(1 → 3)-mannosan.
Extracting the production method of tremella polysaccharides at present, preparation amount is low, and yield is low, is unfavorable for industrialized production.With regard to a kind of method extracting tremella polysaccharides and white fungus albumen of Shandong Light Ind College invention, what they produced is artificial tremella polysaccharides rather than natural wood fungus polysaccharides.
Accordingly, it is desirable to provide a kind of new technical scheme solves the problems referred to above.
Summary of the invention
The technical problem to be solved in the present invention is to provide the production method of a kind of natural silver fungus polysaccharides.It disclosure satisfy that the demand produced above and in scientific research, improves yield, simplifies operation, reduces cost.
The present invention also to solve the technical problem that the purposes being to provide a kind of natural silver fungus polysaccharides in cosmetic material.Have and preferably prevent skin aging effect and moistening effect.
For solving above-mentioned technical problem, the production method of a kind of natural silver fungus polysaccharides of the present invention, comprise the following steps:
(1) selecting without insect pest, nontoxic white fungus dry grinding is standby；
(2) extracting with distilled water to the container dry equipped with white fungus, regulate PH to more than 9.0 with alkali, normal temperature extracts 24-28h；
(3) add resin cation washing after extracting, regulate PH to less than 7.0；
(4) HMW impurity is retained with milipore filter again；
(5) filtrate uses haplo-effect concentrator to be concentrated into proportion is between 1:3-1:5；
(6) concentrate ethanol alcohol precipitation, stirs 1-2h, stands 12-18h；
(7) again with plate type pressure filter, collect white solid, be tremella polysaccharides.
Alkali used is NaOH；Mass concentration 60%-90% that ethanol alcohol precipitation uses.
The application in cosmetic material produces of a kind of natural silver fungus polysaccharides.
Beneficial effects of the present invention: the extraction process of tremella polysaccharides of the present invention is simple, preparation amount is high, yield is high, the common tremella polysaccharides more compared with impurity, tremella polysaccharides purity of the present invention is high, the tremella polysaccharides mentioned in the present invention has antioxidant effect, plays the effect preventing skin aging, although the ultimate density of tremella polysaccharides is the same, but have more excellent anti-oxidant, there is the effect preventing skin aging.
Detailed description of the invention
In order to deepen the understanding of the present invention, below the invention will be further described, this embodiment be only used for explain the present invention, be not intended that the restriction to protection scope of the present invention.
Embodiment 1
The production method of a kind of natural silver fungus polysaccharides of the present invention, comprises the following steps:
(1) selecting without insect pest, nontoxic white fungus dry grinding is standby；
(2) extracting with distilled water to the container dry equipped with white fungus, regulate PH to more than 9.0 with alkali, normal temperature extracts 24h；
(3) add resin cation washing after extracting, regulate PH to less than 7.0；
(4) HMW impurity is retained with milipore filter again；
(5) filtrate uses haplo-effect concentrator to be concentrated into proportion is 1:3；
(6) concentrate ethanol alcohol precipitation, stirs 1h, stands 12h；
(7) again with plate type pressure filter, collect white solid, be tremella polysaccharides.
Alkali used is NaOH；The mass concentration 60% that ethanol alcohol precipitation uses.
Embodiment 2
The production method of a kind of natural silver fungus polysaccharides of the present invention, comprises the following steps:
(1) selecting without insect pest, nontoxic white fungus dry grinding is standby；
(2) extracting with distilled water to the container dry equipped with white fungus, regulate PH to more than 9.0 with alkali, normal temperature extracts 26h；
(3) add resin cation washing after extracting, regulate PH to less than 7.0；
(4) HMW impurity is retained with milipore filter again；
(5) filtrate uses haplo-effect concentrator to be concentrated into proportion is 1:4；
(6) concentrate ethanol alcohol precipitation, stirs 1h, stands 15h；
(7) again with plate type pressure filter, collect white solid, be tremella polysaccharides.
Alkali used is NaOH；The mass concentration 75% that ethanol alcohol precipitation uses.
Embodiment 3
The production method of a kind of natural silver fungus polysaccharides of the present invention, comprises the following steps:
(1) selecting without insect pest, nontoxic white fungus dry grinding is standby；
(2) extracting with distilled water to the container dry equipped with white fungus, regulate PH to more than 9.0 with alkali, normal temperature extracts 28h；
(3) add resin cation washing after extracting, regulate PH to less than 7.0；
(4) HMW impurity is retained with milipore filter again；
(5) filtrate uses haplo-effect concentrator to be concentrated into proportion is 1:5；
(6) concentrate ethanol alcohol precipitation, stirs 2h, stands 18h；
(7) again with plate type pressure filter, collect white solid, be tremella polysaccharides.
Alkali used is NaOH；The mass concentration 90% that ethanol alcohol precipitation uses.
Step (3) uses resin cation to be removed by the alkali in solution, makes solution reduction become colourless transparent liquid.
The mean molecule quantity of the natural silver fungus polysaccharides mentioned in the present invention is 500,000-3,000,000 Da, and its molecular weight is high, is a kind of macromolecule plant source property hyaluronic acid-like, and the water holding capacity of tremella polysaccharides is high, has more preferable skin moisturization.
According to the following experimental example of the present invention, the natural silver fungus polysaccharides preventing skin aging confirmed in the present invention has more excellent moistening effect than common tremella polysaccharides extract.From such experimental result it can be seen that the cosmetic material of the present invention not only has the effect preventing skin aging, and skin moisture-keeping is also had superior effect.(refer to experimental example 5).
Tremella polysaccharides produced according to the invention is made for solution according to muffin 10g/L (ultimate density 1%), and in table 1 below, amount samples (common tremella polysaccharides is filtrate after ultrafiltration).
Experimental example 4: the anti-oxidation efficacy of tremella polysaccharides
According to the present invention, the degree about one-anti-oxidation efficacy in the aging important principle preventing effect of tremella polysaccharides is investigated.1,1-diphenyl-2-picryl hydrazyl (DPPH, Sigma
D9132-1G, USA) make generation free radical in ethanol, compared with common tremella polysaccharides extract, confirm what degree is the amount of the free radical generated be reduced to.In 0.2ml ethanol, tremella polysaccharides in 0.1mM DPPH solution 0.5 that interpolation is prepared with alcohol solvent and the present invention.Then, after above-mentioned solution rotates 10 seconds strongly, 30 minutes the most certainly, under 517nm, measure absorbance with ELISA.
Percentage shown by the degree of the anti-oxidation efficacy of the tremella polysaccharides in the present invention, on the basis of being the photon absorbing intensity of contrast group to use 70% ethanol (without processing group).Its result is as shown in table 2.
[table 2]
The result of 3 groups of Experimental comparison can confirm that anti-oxidation efficacy.For each sample, the anti-oxidation efficacy of embodiment 2-3 is the strongest, and embodiment group 2 is stronger than the anti-oxidation efficacy of embodiment group 1.Experimental result illustrates, containing the anti-oxidation efficacy of tremella polysaccharides of the present invention, has more preferable antioxidant effect than common tremella polysaccharides extract, has the effect preventing skin aging.
Test example 5: tremella polysaccharides moistening effect measures (Corneometer
825)
In order to measure according to above-mentioned table 1 moistening effect, be that object is tested with the men and women 20 (man 5, female 15) of can be affected by experimental result completely 22 ~ 33 years old that does not has dermatopathy.Each experimental subjects, before sample, after first using water cleaning experiment position, then adapts to external environment condition 30 minutes.The environmental condition of experiment is: keeps indoor temperature 22-24 DEG C, indoor humidity 45-50%, and tests in same place.Smear sample each 10 at inboard arm with 2 × 2, and be dried 1 minute.Then respectively coat the ultra-pure water of 20 at the position smeared, be divided into interval (0 point, 1 point, 2 points, 3 points, 4 points, 5 points, 6 points, 7 points, 8 points, 9 points, 10 points) with after towel moisture with 1, use Corneometer CM
825 pairs of each skin surface moisture smearing part are measured.Moistening effect is measured by analyzing.Measurement result such as table 3 so.Unit is Corneometer value (Cv).
[table 3]
As a result of which it is, containing tremella polysaccharides in the present invention, in contrast to common tremella polysaccharides, there is the moistening effect of brilliance.
Claims (3)
1. the production method of a natural silver fungus polysaccharides, it is characterised in that comprise the following steps:
(1) selecting without insect pest, nontoxic white fungus dry grinding is standby；
(2) extracting with distilled water to the container dry equipped with white fungus, regulate PH to more than 9.0 with alkali, normal temperature extracts 24-28h；
(3) add resin cation washing after extracting, regulate PH to less than 7.0；
(4) HMW impurity is retained with milipore filter again；
(5) filtrate uses haplo-effect concentrator to be concentrated into proportion is between 1:3-1:5；
(6) concentrate ethanol alcohol precipitation, stirs 1-2h, stands 12-18h；
(7) again with plate type pressure filter, collect white solid, be tremella polysaccharides.
The production method of a kind of natural silver fungus polysaccharides the most according to claim 1, it is characterised in that: alkali used is NaOH；Mass concentration 60%-90% that ethanol alcohol precipitation uses.
3. the application in cosmetic material produces of a kind of natural silver fungus polysaccharides described in claim 1.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610259099.6A CN105906731A (en) | 2016-04-25 | 2016-04-25 | Production method and use of natural tremella polysaccharide |
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| CN201610259099.6A CN105906731A (en) | 2016-04-25 | 2016-04-25 | Production method and use of natural tremella polysaccharide |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106905440A (en) * | 2017-04-14 | 2017-06-30 | 中国林业科学研究院林产化学工业研究所 | The new method that a kind of tremella polysaccharides is extracted |
| CN107383228A (en) * | 2017-08-18 | 2017-11-24 | 合肥丰洁生物科技有限公司 | A kind of method for extracting high purity of tremella polysaccharides |
| CN108359025A (en) * | 2018-04-13 | 2018-08-03 | 苏州尚格生物科技有限公司 | Tremella polysaccharides and the preparation method and application thereof |
| CN113274333A (en) * | 2021-04-16 | 2021-08-20 | 云南白药集团股份有限公司 | Pineapple extract composition with antioxidation effect, and preparation method and application thereof |
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| CN101434662A (en) * | 2008-12-29 | 2009-05-20 | 河南工业大学 | Method for preparing fungal polysaccharide |
| CN101597339A (en) * | 2009-07-03 | 2009-12-09 | 四川大学 | A kind of is the method for feedstock production tremella polysaccharide with white fungus, defective goods and ear pin |
| US20120009241A1 (en) * | 2010-07-12 | 2012-01-12 | Agricultural Research Institute | Skin wound dressing and preparing method thereof |
| CN104211829A (en) * | 2014-09-17 | 2014-12-17 | 上海交通大学 | Preparation and application of white fungus functional polysaccharide |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101434662A (en) * | 2008-12-29 | 2009-05-20 | 河南工业大学 | Method for preparing fungal polysaccharide |
| CN101597339A (en) * | 2009-07-03 | 2009-12-09 | 四川大学 | A kind of is the method for feedstock production tremella polysaccharide with white fungus, defective goods and ear pin |
| US20120009241A1 (en) * | 2010-07-12 | 2012-01-12 | Agricultural Research Institute | Skin wound dressing and preparing method thereof |
| CN104211829A (en) * | 2014-09-17 | 2014-12-17 | 上海交通大学 | Preparation and application of white fungus functional polysaccharide |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106905440A (en) * | 2017-04-14 | 2017-06-30 | 中国林业科学研究院林产化学工业研究所 | The new method that a kind of tremella polysaccharides is extracted |
| CN106905440B (en) * | 2017-04-14 | 2019-03-19 | 中国林业科学研究院林产化学工业研究所 | A kind of method that tremella polysaccharides extract |
| CN107383228A (en) * | 2017-08-18 | 2017-11-24 | 合肥丰洁生物科技有限公司 | A kind of method for extracting high purity of tremella polysaccharides |
| CN108359025A (en) * | 2018-04-13 | 2018-08-03 | 苏州尚格生物科技有限公司 | Tremella polysaccharides and the preparation method and application thereof |
| CN113274333A (en) * | 2021-04-16 | 2021-08-20 | 云南白药集团股份有限公司 | Pineapple extract composition with antioxidation effect, and preparation method and application thereof |
| CN113274333B (en) * | 2021-04-16 | 2022-07-26 | 云南白药集团股份有限公司 | Pineapple extract composition with antioxidation effect, and preparation method and application thereof |
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