NL2033639B1 - Polysaccharide Composition with Moisturizing, Repairing and Anti-aging Functions - Google Patents

Polysaccharide Composition with Moisturizing, Repairing and Anti-aging Functions Download PDF

Info

Publication number
NL2033639B1
NL2033639B1 NL2033639A NL2033639A NL2033639B1 NL 2033639 B1 NL2033639 B1 NL 2033639B1 NL 2033639 A NL2033639 A NL 2033639A NL 2033639 A NL2033639 A NL 2033639A NL 2033639 B1 NL2033639 B1 NL 2033639B1
Authority
NL
Netherlands
Prior art keywords
polysaccharide
migo
batsch
repairing
moisturizing
Prior art date
Application number
NL2033639A
Other languages
Dutch (nl)
Inventor
Zhu Jing
Original Assignee
Wuxi Ary Biotechnology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wuxi Ary Biotechnology Co Ltd filed Critical Wuxi Ary Biotechnology Co Ltd
Priority to NL2033639A priority Critical patent/NL2033639B1/en
Application granted granted Critical
Publication of NL2033639B1 publication Critical patent/NL2033639B1/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Dermatology (AREA)
  • Engineering & Computer Science (AREA)
  • Gerontology & Geriatric Medicine (AREA)
  • Biotechnology (AREA)
  • Botany (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Birds (AREA)
  • Epidemiology (AREA)
  • Cosmetics (AREA)

Abstract

The present disclosure belongs to the field of skin care products, and particularly discloses a polysaccharide composition with moisturizing, repairing and anti-aging functions. The polysaccharide composition includes Dendrobium officinale Kimura et Migo polysaccharide, Prunus persica (L.) Batsch polysaccharide, Polygonatum odoratum (Mill. ) Druce polysaccharide and Glycyrrhiza uralensis Fisch. polysaccharide, wherein the ratio of the Dendrobium officina/e Kimura et Migo polysaccharide to the Prunus persica (L.) Batsch polysaccharide to the Polygonatum odoratum (Mi/l.) Druce polysaccharide to the Glycyrrhiza ura/ensis Fisch. polysaccharide is 1: (0.3-0.6): (0.5-1): (0.8-1.2). The polysaccharide composition with moisturizing, repairing and anti-aging functions provided by the present disclosure includes the Dendrobium officinale Kimura et Migo polysaccharide, the Prunus persica (L.) Batsch polysaccharide, the Polygonatum odoratum (Mi/l.) Druce polysaccharide and the Glycyrrhiza ura/ensis Fisch. polysaccharide; and the polysaccharide composition has a synergistic effect on expression of aquaporin, and can improve water transmission and/or wetting agent transmission in skin, prevent and/or treat diseases caused by skin barrier damage and prevent and/or treat sicca syndrome, thus achieving the effects of repairing mucosa and relieving skin dryness and aging.

Description

Polysaccharide Composition with Moisturizing, Repairing and Anti-aging Functions
Technical Field
The present disclosure relates to the field of skin care products, and particularly relates to a polysaccharide composition with moisturizing, repairing and anti-aging functions.
Background
With the increase of the age and the influence of the external environment, the moisturizing mechanism of the skin will be damaged, and the moisture content among skin tissue cells will be reduced, resulting in tight arrangement of the cells and dehydrating and hardening of collagen. If the moisture content in the cuticle is reduced to 10% or below, the skin will be dry, lose elasticity and wrinkle, and the skin aging process will be accelerated. Therefore, the moisture is crucial to skin health, and water retention and moisturizing are one of the most important research subjects of skin care cosmetics all the time. Research shows that the sufficient moisture content is an important condition for keeping the skin healthy and delaying aging. The moisturizing cosmetics can maintain the skin moisture by simulating a natural skin moisturizing system through different mechanisms, including reducing percutaneous moisture loss, absorbing external moisture, achieving hydration moisturizing, repairing keratinocytes, etc.
At present, the best moisturizing substance in the nature is hyaluronic acid, which is known as the most ideal natural moisturizing factor by the international cosmetic industry, and is an acidic mucopolysaccharide. However, because the source of hyaluronic acid is special, hyaluronic acid cannot meet the market demand, and it is urgently needed to find a suitable substitute to solve this problem. The natural plant polysaccharide is a good substitute product.
Polysaccharide has good film forming property, and can form a layer of uniform film on the surface of skin to reduce moisture evaporation on the surface of the skin. In addition, in the extracellular matrix, the polysaccharide can work together with other polysaccharide components in the skin and fibrous protein to form an extracellular colloidal matrix containing a large amount of moisture, and moisture can also be provided for the skin. The polysaccharide applied to cosmetics has the advantages that on one hand, the polysaccharide is wide in effect and can achieve the highly efficient skin care and skin beautifying effects in multiple aspects.
For example, the polysaccharide has the effects of moisturizing, resisting oxidation, resisting aging, enhancing immunity, blocking sun, repairing skin tissue, promoting skin blood circulation, resisting acne and the like, can achieve the effects of moisturizing, resisting bacteria and preventing ultraviolet radiation when acting on the surface of the skin, and can promote skin microcirculation, resist oxidation, delay skin aging and repair damaged skin when acting on the inner layer of the skin. On the other hand, the polysaccharide is small in toxic and side effects, small in harm to human bodies and high in safety.
The inventor finds that the composition in the present disclosure has obvious effects in the aspects of hydrating and moisturizing, and the effects can be achieved through the synergistic effect among components, so that the composition can be used for providing the skin hydrating effect for mammals.
Summary
The present disclosure aims to provide a polysaccharide compasition with moisturizing, repairing and anti-aging functions to solve the problems in the Background.
In order to achieve above purpose, the present disclosure provides the following technical solution: a polysaccharide composition with moisturizing, repairing and anti-aging functions includes Dendrobium officinale Kimura et Migo polysaccharide, Prunus persica (L.) Batsch polysaccharide, Polygonatum odoratum (Mill) Druce polysaccharide and Glycyrrhiza uralensis
Fisch. polysaccharide, wherein the ratio of the Dendrobium officinale Kimura ef Migo polysaccharide to the Prunus persica (L.) Batsch polysaccharide to the Polygonatum odoratum (Mill) Druce polysaccharide to the Glycyrrhiza uralensis Fisch. polysaccharide is 1: (0.3-0.6): (0.5-1): (0.8-1.2).
Preferably, the ratio of the Dendrobium officinale Kimura et Migo polysaccharide to the
Prunus persica (L.) Batsch polysaccharide to the Polygonatum odoratum (Mill.) Druce polysaccharide to the Glycyrrhiza uralensis Fisch. polysaccharide is 1: (0.3-0.4): (0.6-0.9): (0.8- 1.1).
Preferably, the Dendrobium officinale Kimura et Migo polysaccharide, the Prunus persica (L.) Batsch polysaccharide, the Polygonatum odoratum (Mill) Druce polysaccharide and the
Glycyrrhiza uralensis Fisch. polysaccharide are all large molecular polysaccharide, wherein the
Dendrobium officinale Kimura et Migo polysaccharide is extracted from three-year-old
Dendrobium officinale Kimura et Migo harvested from November to March.
Preferably, a method for extracting the Dendrobium officinale Kimura et Migo polysaccharide includes the steps of: crushing and screening Dendrobium officinale Kimura et
Migo with a 20-40-mesh sieve; putting the powder into water with the amount 10 times that of the powder; adding cellulase and papain; regulating the PH to be 6; performing enzymolysis at the water temperature of 40°C for 3 h; then increasing the temperature to 100°C; performing extraction for 2 h; and performing extraction twice to obtain the Dendrobium officinale Kimura et
Migo polysaccharide.
Preferably, a method for extracting the Prunus persica (L.) Batsch polysaccharide includes the steps of: removing impurities from collected Prunus persica (L.) Batsch; performing rinsing; then performing freeze drying; crushing the Prunus persica (L.) Batsch and screening with the 20-40-mesh sieve; putting the powder into water with the amount 10 times that of the powder; performing extraction at 90°C for 2 h; performing centrifugation and then performing vacuum concentration on supernatant; performing deproteinization on the concentrated solution through a Sevage method; adding an absolute ethyl alcohol solution with the volume 4 times that of deproteinized polysaccharide liquid; placing the mixture overnight at the room temperature; performing centrifugation to obtain a precipitate; and performing vacuum drying to obtain the
Prunus persica {L.) Batsch polysaccharide.
Preferably, a method for extracting and releasing the Polygonatum odoratum (Mill.) Druce polysaccharide includes the steps of: crushing Polygonatum odoratum (Mill) Druce and screening with the 20-40-mesh sieve; performing extraction with an ethanol solution with a volume concentration of 85% at 90°C for 2 h; removing monosaccharide, oligosaccharide and fat; weighing a certain amount of a pre-treated dry sample; putting the sample into a beaker; adding deionized water according to a material-liquid ratio of 1: 40 (g/ml); putting the beaker into an ultrasonic cell disruptor, and performing extraction for 20 min, wherein the ultrasonic power is 400 W; performing high-speed centrifugation, filtration and vacuum concentration on an extracting solution; then precipitating with ethanol with a volume concentration of 85%; and performing freeze drying on the precipitate to obtain the Polygonatum odoratum (Mill) Druce polysaccharide.
Preferably, a method for extracting the Glycyrrhiza uralensis Fisch. polysaccharide includes the steps of: crushing Glycyrrhiza uralensis Fisch. and screening with the 20-40-mesh sieve; putting the powder into water with the amount 10 times that of the powder; performing ultrasonic extraction in a water bath at 45°C for 20 min; performing filtration; putting filter residues into water with the amount 10 times that of the filter residues; repeating ultrasonic extraction twice; combining extraction solutions obtained by three times of extraction; performing reduced pressure concentration on a rotary evaporator to 50 ml; cooling at room temperature; performing centrifugation on the concentrated solution at 5,000 r/min for 5 min; removing impurity precipitates; slowly adding 95% ethanol with the amount 4 times that of the concentration solution; continuously stirring with a glass rod to fully separate out Glycyrrhiza uralensis Fisch. polysaccharide; standing for 24 h; performing centrifugation at 5,000 r/min for 5 min to obtain a Glycyrrhiza uralensis Fisch. polysaccharide precipitate; washing with absolute ethyl alcohol for 2-3 times; and drying at 60°C to obtain the Glycyrrhiza uralensis Fisch. polysaccharide.
Compared with the prior art, the present disclosure has the beneficial effects that the polysaccharide composition with moisturizing, repairing and anti-aging functions provided by the present disclosure includes the Dendrobium officinale Kimura et Migo polysaccharide, the
Prunus persica (L.) Batsch polysaccharide, the Polygonatum odoratum (Mill) Druce polysaccharide and the Glycyrrhiza uralensis Fisch. polysaccharide; and the polysaccharide composition has a synergistic effect on expression of aquaporin, and can improve water transmission and/or wetting agent transmission in skin, prevent and/or treat diseases caused by skin barrier damage and prevent and/or treat sicca syndrome, thus achieving the effects of repairing mucosa and relieving skin dryness and aging.
Detailed Description
The technical solutions in the embodiments of the present disclosure will be described clearly and completely hereinafter with reference to the drawings in the embodiments of the present disclosure. Obviously, the described embodiments are only part of the embodiments of the present disclosure, not all of them. Based on the embodiments of the present disclosure, all other embodiments obtained by ordinary technicians in the art without creative work belong to the scope of the present disclosure.
In the following embodiments, Dendrobium officinale Kimura et Migo polysaccharide,
Prunus persica (L.) Batsch polysaccharide, Polygonatum odoratum (Mill) Druce polysaccharide and Glycyrrhiza uralensis Fisch. polysaccharide are all large molecular polysaccharide; and the
Dendrobium officinale Kimura et Migo polysaccharide is extracted from three-year-old
Dendrobium officinale Kimura et Migo harvested from November to March. The preparation methods of the Dendrobium officinale Kimura et Migo polysaccharide, the Prunus persica (L.)
Batsch polysaccharide, the Polygonatum odoratum (Mill.) Druce polysaccharide and the
Glycyrrhiza uralensis Fisch. polysaccharide are as follows:
A method for extracting the Dendrobium officinale Kimura et Migo polysaccharide includes the steps of: crushing and screening Dendrobium officinale Kimura et Migo with a 20-40-mesh sieve; putting the powder into water with the amount 10 times that of the powder; adding cellulase and papain; regulating the PH to be 8; performing enzymolysis at the water temperature of 40°C for 3 h; then increasing the temperature to 100°C; performing extraction for 2 h; and performing extraction twice to obtain the Dendrobium officinale Kimura et Migo polysaccharide.
A method for extracting the Prunus persica (L.) Batsch polysaccharide includes the steps of: removing impurities from collected Prunus persica (L.) Batsch; performing rinsing; then performing freeze drying; crushing the Prunus persica (L.) Batsch and screening with the 20-40- mesh sieve; putting the powder into water with the amount 10 times that of the powder; performing extraction at 90°C for 2 h; performing centrifugation and then performing vacuum concentration on supernatant; performing deproteinization on the concentrated solution through a Sevage method; adding an absolute ethyl alcohol solution with the volume 4 times that of deproteinized polysaccharide liquid; placing the mixture overnight at the room temperature; performing centrifugation to obtain a precipitate; and performing vacuum drying to obtain the
Prunus persica (L.) Batsch polysaccharide.
A method for extracting and releasing the Polygonatum odoratum (Mill.) Druce polysaccharide includes the steps of: crushing Polygonatum odoratum (Mill.) Druce and screening with the 20-40-mesh sieve; performing extraction with an ethanol solution with a volume concentration of 85% at 90°C for 2 h; removing monosaccharide, oligosaccharide and fat; weighing a certain amount of a pre-treated dry sample; putting the sample into a beaker, adding deionized water according to a material-liquid ratio of 1: 40 (g/ml); putting the beaker into an ultrasonic cell disruptor, and performing extraction for 20 min, wherein the ultrasonic power is 400 W; performing high-speed centrifugation, filtration and vacuum concentration on an extracting solution; then precipitating with ethanol with a volume concentration of 85%; and performing freeze drying on the precipitate to obtain the Polygonatum odoratum (Mill) Druce 5 polysaccharide.
A method for extracting the Glycyrrhiza uralensis Fisch. polysaccharide includes the steps of: crushing Glycyrrhiza uralensis Fisch. and screening with the 20-40-mesh sieve; putting the powder into water with the amount 10 times that of the powder; performing ultrasonic extraction in a water bath at 45°C for 20 min; performing filtration; putting filter residues into water with the amount 10 times that of the filter residues; repeating ultrasonic extraction twice; combining extraction solutions obtained by three times of extraction; performing reduced pressure concentration on a rotary evaporator to 50 ml; cooling at room temperature; performing centrifugation on the concentrated solution at 5,000 r/min for 5 min; removing impurity precipitates; slowly adding 95% ethanol with the amount 4 times that of the concentration solution; continuously stirring with a glass rod to fully separate out Glycyrrhiza uralensis Fisch. polysaccharide; standing for 24 h; performing centrifugation at 5,000 r/min for 5 min to obtain a
Glycyrrhiza uralensis Fisch. polysaccharide precipitate; washing with absolute ethyl alcohol for 2-3 times; and drying at 60°C to obtain the Glycyrrhiza uralensis Fisch. polysaccharide.
Embodiment 1:
A polysaccharide composition with moisturizing, repairing and anti-aging functions included
Dendrobium officinale Kimura et Migo polysaccharide, Prunus persica (L.) Batsch polysaccharide, Polygonatum odoratum (Mill) Druce polysaccharide and Glycyrrhiza uralensis
Fisch. polysaccharide, wherein the ratio of the Dendrobium officinale Kimura et Migo polysaccharide to the Prunus persica (L.) Batsch polysaccharide to the Polygonatum odoratum (Mill) Druce polysaccharide to the Glycyrrhiza uralensis Fisch. polysaccharide was 1: 0.4: 0.6: 1.1.
Embodiment 2:
A polysaccharide composition with moisturizing, repairing and anti-aging functions included
Dendrobium officinale Kimura et Migo polysaccharide, Prunus persica (L.) Batsch polysaccharide, Polygonatum odoratum (Mill) Druce polysaccharide and Glycyrrhiza uralensis
Fisch. polysaccharide, wherein the ratio of the Dendrobium officinale Kimura et Migo polysaccharide to the Prunus persica (L.) Batsch polysaccharide to the Polygonatum odoratum (Mill) Druce polysaccharide to the Glycyrrhiza uralensis Fisch. polysaccharide was 1: 0.3: 0.8: 0.9.
Experimental embodiment:
An experimental product 1 obtained in the Embodiment 1 and an experimental product 2 obtained in the Embodiment 2 were taken to be experimented with a contrast product 1 and a contrast product 2, the contrast product 1 in the Experimental Embodiment was hyaluronic acid with small molecular weight of 1%, and the contrast product 2 was hyaluronic acid with medium molecular weight of 1%. An experimental method included the steps of: screening suitable subjects, cleaning inner sides of forearms, and marking test areas; making the subjects to statically sit under a constant temperature and humidity condition for 20 min, and measuring the initial values of the skin cuticle moisture content and the skin percutaneous moisture loss amount; smearing to-be-tested samples with a concentration of (2.00.1) mg/cm? in the test areas according to a sample random distribution table; and measuring the skin cuticle moisture content and the skin percutaneous moisture loss amount after the polysaccharide composition was used for1h, 2h, 4h, 6h, 8 hand 12 h. The results are shown in the following Table 1 and
Table 2.
Table 1: Rate of change in skin cuticle moisture content
Experimental Experimental Contrast Contrast Blank [sert | ette | pet | te | on
The Table 1 was a table showing the change in moisture content of different samples over time. As shown in the table, the hydrating effects of the experimental product 1 and the experimental product 2 were obviously superior to that of the hyaluronic acid with medium molecular weight of 1% and slightly superior to that of the hyaluronic acid with small molecular weight of 1%, and the hydrating duration of the experimental product 1 and the experimental product 2 was obviously longer than that of the hyaluronic acid with medium molecular weight of 1% and the hyaluronic acid with small molecular weight of 1%.
Table 2: Rate of change in skin cuticle moisture content
Experimental Experimental Contrast Contrast Blank
EEE | ans | on
The Table 2 was a table showing the change in percutaneous moisture loss of different samples over time. As shown in the table, the moisture retention effects of the experimental product 1 and the experimental product 2 were obviously superior to that of the hyaluronic acid with medium molecular weight of 1% and slightly superior to that of the hyaluronic acid with small molecular weight of 1%, and the moisture retention duration of the experimental product 1 and the experimental product 2 was obviously longer than that of the hyaluronic acid with medium molecular weight of 1% and the hyaluronic acid with small molecular weight of 1%.
Although embodiments of the present disclosure have been shown and described, it can be understood for those skilled in the art that a variety of changes, modifications, substitutions and modifications can be made to these embodiments without departing from the principles and spirit of the present disclosure. The scope of the present disclosure is defined by the appended claims and their equivalents.

Claims (7)

CONCLUSIESCONCLUSIONS 1. Een polysacharidesamenstelling met hydraterende, herstellende en anti- verouderende eigenschappen, welke samenstelling Dendrobium officinale Kimura et Migo polysacharide, Prunus persica (L) Batsch polysaccharide, Polygonatum odoratum (Mill) Druce polysaccharide en G/ycyrrhiza uralensis Fisch. polysacharide omvat, waarbij de verhouding tussen het Dendrobium officinale Kimura et Migo polysacharide, Prunus persica (L.) Batsch polysacharide, Polygonatum odoratum (Mill.) Druce polysacharide en Glycyrrhiza uralensis Fisch polysacharide 1 : (0,3 - 0,6) : (0.5-1) : (0.8 - 1.2) bedraagt.1. A polysaccharide composition with moisturizing, repairing and anti-aging properties, which composition contains Dendrobium officinale Kimura et Migo polysaccharide, Prunus persica (L) Batsch polysaccharide, Polygonatum odoratum (Mill) Druce polysaccharide and G/ycyrrhiza uralensis Fisch. polysaccharide, where the ratio between the Dendrobium officinale Kimura et Migo polysaccharide, Prunus persica (L.) Batsch polysaccharide, Polygonatum odoratum (Mill.) Druce polysaccharide and Glycyrrhiza uralensis Fisch polysaccharide 1 : (0.3 - 0.6) : ( 0.5-1) : (0.8 - 1.2). 2. De polysaccharidesamenstelling met hydraterende, herstellende en anti-verouderende eigenschappen volgens conclusie 2, waarbij de verhouding tussen het Dendrobium officinale Kimura et Migo polysacharide, Prunus persica (L) Batsch polysacharide, Polygonatum odoratum (Mill) Druce polysacharide en Glycyrrhiza uralensis Fisch polysacharide 1: (0,3 - 0,4) : (0.6 - 0.9) : (0.8 - 1.1) bedraagt.The polysaccharide composition with moisturizing, repairing and anti-aging properties according to claim 2, wherein the ratio between the Dendrobium officinale Kimura et Migo polysaccharide, Prunus persica (L) Batsch polysaccharide, Polygonatum odoratum (Mill) Druce polysaccharide and Glycyrrhiza uralensis Fisch polysaccharide 1 : (0.3 - 0.4) : (0.6 - 0.9) : (0.8 - 1.1). 3. De polysaccharidesamenstelling met hydraterende, herstellende en anti-verouderende eigenschappen volgens conclusie 1, waarbij het Dendrobium officinale Kimura et Migo polysacharide, Prunus persica (L.) Batsch polysaccharide, Polygonatum odoratum (Mill.) Druce polysaccharide en Glycyrrhiza uralensis Fisch. polysacharide allemaal hoogmoleculaire polysacchariden zijn; en het Dendrobium officinale Kimura et Migo polysacharide geëxtraheerd is uit drie jaar oude Dendrobium officinale Kimura et Migo die tussen november en maart is geoogst.The polysaccharide composition with moisturizing, repairing and anti-aging properties according to claim 1, wherein the Dendrobium officinale Kimura et Migo polysaccharide, Prunus persica (L.) Batsch polysaccharide, Polygonatum odoratum (Mill.) Druce polysaccharide and Glycyrrhiza uralensis Fisch. polysaccharides are all high molecular weight polysaccharides; and the Dendrobium officinale Kimura et Migo polysaccharide has been extracted from three-year-old Dendrobium officinale Kimura et Migo harvested between November and March. 4. De polysaccharidesamenstelling met hydraterende, herstellende en anti-verouderende eigenschappen volgens conclusie 1, waarbij de werkwijze voor het extraheren van het Dendrobium officinale Kimura et Migo polysacharide de volgende stappen omvat: — het malen en zeven van Dendrobium officinale Kimura et Migo met een zeef van 20 - 40 mesh; — het toevoegen van het poeder in water, waarbij de hoeveelheid water 10 keer zo groot is als die van het poeder; — het toevoegen van cellulase en papaïne; — het instellen van de PH regelen op 6; — het gedurende 3 uur uitvoeren van enzymolyse bij een watertemperatuur van 40°C; — vervolgens het verhogen van de temperatuur verhogen tot 100°C; — het gedurende 2 uur uitvoeren van extractie uitvoeren; en — het twee keer uitvoeren van de extractie tweemaal uitvoeren om het Dendrobium officinale Kimura et Migo polysacharide te verkrijgen.The polysaccharide composition with moisturizing, repairing and anti-aging properties according to claim 1, wherein the method of extracting the Dendrobium officinale Kimura et Migo polysaccharide comprises the following steps: - grinding and sieving Dendrobium officinale Kimura et Migo with a sieve from 20 - 40 mesh; — adding the powder in water, the amount of water being 10 times that of the powder, — adding cellulase and papain; — adjusting the PH to 6; — carrying out enzymolysis at a water temperature of 40°C for 3 hours; — then increase the temperature to 100°C; — carry out extraction for 2 hours; and — performing the extraction twice to obtain the Dendrobium officinale Kimura et Migo polysaccharide. 5. De polysaccharidesamenstelling met hydraterende, herstellende en anti-verouderende eigenschappen volgens conclusie 1, waarbij de werkwijze voor het extraheren van de Prunus persica (L.) Batsch polysaccharide de volgende stappen omvat: — het verwijderen van onzuiverheden uit de verzamelde Prunus persica (L.) Batsch; — het spoelen en vervolgens vriesdrogen; — het vermalen van de Prunus persica (L.) Batsch en het zeven met een zeef van 20 — 40 mesh; — het toevoegen van het poeder in water, waarbij de hoeveelheid water 10 keer zo groot is als die van het poeder; — het gedurende twee uur bij 90°C extraheren; — het centrifugeren en vervolgens vacuümconcentratie van de bovenstaande vloeistof; — het uit de geconcentreerde oplossing verwijderen van eiwit volgens de Sevage-methode; — het toevoegen van absolute ethanol, waarbij het volume van het ethanol vier keer zo groot is als dat van de polysacharidevloeistof waaruit het eiwit is verwijderd; — het gedurende een nacht bij kamertemperatuur laten staan van het mengsel; — het centrifugeren om een neerslag te verkrijgen; en — het vacuümdrogen om het Prunus persica (L.) Batsch polysacharide te verkrijgen.The polysaccharide composition with moisturizing, repairing and anti-aging properties according to claim 1, wherein the method of extracting the Prunus persica (L.) Batsch polysaccharide comprises the following steps: - removing impurities from the collected Prunus persica (L. .) Batsch; — rinsing and subsequent freeze-drying; — grinding the Prunus persica (L.) Batsch and sieving with a 20 — 40 mesh sieve; — adding the powder in water, the amount of water being 10 times that of the powder, — extract it at 90°C for two hours; — centrifugation and subsequent vacuum concentration of the supernatant; — removing protein from the concentrated solution by the Sevage method; — adding absolute ethanol, the volume of the ethanol being four times that of the polysaccharide liquid from which the protein has been removed, — leaving the mixture at room temperature overnight; — centrifugation to obtain a precipitate; and — vacuum drying to obtain the Prunus persica (L.) Batsch polysaccharide. 6. De polysaccharidesamenstelling met hydraterende, herstellende en anti-verouderende eigenschappen volgens conclusie 1, waarbij de werkwijze voor het extraheren en vrijmaken van het Polygonatum odoratum (Mill) Druce polysacharide de volgende stappen omvat: — het vermalen van Polygonatum odoratum (Mill.) Druce en het zeven met een zeef van 20 — 40 mesh; — het gedurende 2 uur bij 20°C uitvoeren van extractie met een ethanoloplossing met een volumeconcentratie van 85%; — het verwijderen van monosacharide, oligosacharide en vet; — het wegen van een bepaalde hoeveelheid van een voorbehandeld droog monster; — het overbrengen van het monster in een bekerglas; — het toevoegen van gedeioniseerd water in een verhouding vaste stof - vloeistof van 1 : 40 g/ml; — het plaatsen het bekerglas in een ultrasone celbreker en uitvoeren van extractie gedurende 20 minuten, waarbij het ultrasone vermogen 400 W bedraagt; — het centrifugeren, filteren en vacuümconcentreren van de extractieoplossing met hoge snelheid; vervolgens — het neerslaan met ethanol met een volumeconcentratie van 85%; enThe polysaccharide composition with moisturizing, repairing and anti-aging properties according to claim 1, wherein the method for extracting and releasing the Polygonatum odoratum (Mill.) Druce polysaccharide comprises the following steps: - grinding Polygonatum odoratum (Mill.) Druce and sieving with a 20 — 40 mesh sieve; — performing extraction with an ethanol solution of 85% volume concentration for 2 hours at 20°C; — the removal of monosaccharide, oligosaccharide and fat; — weighing a certain quantity of a pre-treated dry sample; — transferring the sample into a beaker; — adding deionized water in a solid to liquid ratio of 1:40 g/ml; — placing the beaker in an ultrasonic cell crusher and performing extraction for 20 minutes, with the ultrasonic power being 400 W; — high-speed centrifugation, filtering and vacuum concentration of the extraction solution; then — precipitation with ethanol with a volume concentration of 85%; and — het vriesdrogen van het neerslag om het Polygonatum odoratum (Mill) Druce polysaccharide te verkrijgen.— freeze-drying the precipitate to obtain the Polygonatum odoratum (Mill) Druce polysaccharide. 7. De polysaccharidesamenstelling met hydraterende, herstellende en anti-verouderende eigenschappen volgens conclusie 1, waarbij de werkwijze voor het extraheren en vrijmaken van het Glycyrrhiza uralensis Fisch. polysacharide de volgende stappen omvat: — het vermalen van Glycyrrhiza uralensis Fisch. en het zeven met een zeef van 20-40 mesh; — het toevoegen van het poeder in water, waarbij de hoeveelheid water 10 keer zo groot is als die van het poeder; — het gedurende 20 minuten uitvoeren van ultrasone extractie in een waterbad van 45°C; — het uitvoeren van filtratie; — het in water brengen van de filtratieresten, waarbij de hoeveelheid water 10 keer zo groot is als die van de filterresten; — het tweemaal herhalen van de ultrasone extractie; — het combineren van de extractieoplossingen verkregen door driemaal extractie; — het bij verminderde druk in een rotatieverdamper concentreren tot 50 mi; — het afkoelen bij kamertemperatuur; — het centrifugeren van de geconcentreerde oplossing bij 5.000 omw/min gedurende 5 minuten; — het verwijderen van verontreinigende precipitaten; — het langzaam toevoegen van 95% ethanol waarbij de hoeveelheid ethanol 4 keer zo groot is als die van de geconcentreerde oplossing; — het voortdurend roeren met een glazen staaf om het G/ycyrrhiza uralensis Fisch. polysaccharide te scheiden; — het laten staan geduernde 24 uur; — het centrifugeren bij 5.000 omw/min gedurende 5 minuten om een neerslag van Glycyrrhiza uralensis Fisch. polysacharide te verkrijgen; — het 2 - 3 maal wassen met absolute ethanol; en — het drogen bij 60°C om de Glycyrrhiza uralensis Fisch. polysaccharide te verkrijgen.The polysaccharide composition with moisturizing, repairing and anti-aging properties according to claim 1, wherein the method of extracting and releasing the Glycyrrhiza uralensis Fisch. polysaccharide involves the following steps: — grinding Glycyrrhiza uralensis Fisch. and sieving with a 20-40 mesh sieve; — adding the powder in water, the amount of water being 10 times that of the powder, — performing ultrasonic extraction in a water bath at 45°C for 20 minutes; — carrying out filtration; — introducing the filtration residues into water, whereby the amount of water is 10 times that of the filter residues; — repeating the ultrasonic extraction twice; — combining the extraction solutions obtained by three times extraction; — concentrating to 50 ml at reduced pressure in a rotary evaporator; — cooling at room temperature; — centrifuging the concentrated solution at 5,000 rpm for 5 minutes; — the removal of contaminating precipitates; — slowly adding 95% ethanol, the amount of ethanol being 4 times that of the concentrated solution; — continuous stirring with a glass rod to stir the G/ycyrrhiza uralensis Fisch. to separate polysaccharide; — leave it for 24 hours; — centrifugation at 5,000 rpm for 5 minutes to remove a precipitate of Glycyrrhiza uralensis Fisch. to obtain polysaccharide; — washing 2 - 3 times with absolute ethanol; and — drying at 60°C to obtain the Glycyrrhiza uralensis Fisch. to obtain polysaccharide.
NL2033639A 2022-11-30 2022-11-30 Polysaccharide Composition with Moisturizing, Repairing and Anti-aging Functions NL2033639B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
NL2033639A NL2033639B1 (en) 2022-11-30 2022-11-30 Polysaccharide Composition with Moisturizing, Repairing and Anti-aging Functions

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
NL2033639A NL2033639B1 (en) 2022-11-30 2022-11-30 Polysaccharide Composition with Moisturizing, Repairing and Anti-aging Functions

Publications (1)

Publication Number Publication Date
NL2033639B1 true NL2033639B1 (en) 2023-08-15

Family

ID=84569259

Family Applications (1)

Application Number Title Priority Date Filing Date
NL2033639A NL2033639B1 (en) 2022-11-30 2022-11-30 Polysaccharide Composition with Moisturizing, Repairing and Anti-aging Functions

Country Status (1)

Country Link
NL (1) NL2033639B1 (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4179971B2 (en) * 2003-11-17 2008-11-12 小川香料株式会社 Moisturizing plant extract and external preparation, cosmetic, bath preparation and detergent containing the extract
CN103230548A (en) * 2013-05-06 2013-08-07 孙永权 Traditional Chinese medicine composition for treating pulmonary heart disease

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4179971B2 (en) * 2003-11-17 2008-11-12 小川香料株式会社 Moisturizing plant extract and external preparation, cosmetic, bath preparation and detergent containing the extract
CN103230548A (en) * 2013-05-06 2013-08-07 孙永权 Traditional Chinese medicine composition for treating pulmonary heart disease

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
DATABASE GNPD [online] MINTEL; 12 May 2021 (2021-05-12), ANONYMOUS: "Toner", XP093052362, retrieved from https://www.gnpd.com/sinatra/recordpage/8655403/ Database accession no. 8655403 *

Similar Documents

Publication Publication Date Title
CN111407719B (en) Skin barrier repair compound, face cream and preparation method thereof
Liu et al. Antihyperglycemic, antihyperlipidemic and antioxidant activities of polysaccharides from Catathelasma ventricosum in streptozotocin-induced diabetic mice
CN112516067A (en) Composition containing umbilical cord mesenchymal stem cell exosome and application thereof
CN109172432A (en) Moisturizing remediation composition and its application
CN106420500A (en) Composition containing inonotus obliquus extract and application of composition
JPH11313667A (en) Liquid culture of schizophyllum commune fr. for separation of beta-1,6-branched-1,3-glucan, and preparation composition for external use, containing beta-1,6-branched-1,3-glucan produced by the liquid culture
CN108042436B (en) Anti-aging and moisturizing blue copper peptide essence cream
CN112754956A (en) Formula and preparation method of cactus polysaccharide anti-inflammatory moisturizing mask
CN108721139A (en) A kind of moisture-keeping composition containing polysaccharide
CN115137673A (en) Fermented cosmetic composition and preparation method and application thereof
NL2033639B1 (en) Polysaccharide Composition with Moisturizing, Repairing and Anti-aging Functions
CN115154363B (en) Mulberry resource oligosaccharide moisturizing composition and preparation method and application thereof
CN114272165B (en) Skin-whitening and tightening plant essence skin-care emulsion and preparation method thereof
CN113244156B (en) Anti-aging composition and application thereof
CN116270392A (en) Composition with moisturizing effect and application thereof
CN112779305A (en) Preparation method of cistanche enzymolysis oligosaccharide concentrated solution, obtained product and application
CN115887287A (en) Whitening, moisturizing and anti-aging mask liquid containing plant polysaccharide and preparation method thereof
KR102654331B1 (en) Preparation and application of a fermentation composition with anti-photoaging,calming,cooling effects
CN109453104B (en) Spot-fading skin-refreshing essence and preparation process thereof
CN117414312B (en) Preparation method and application of hydrolyzed sclerotium rolfsii with repairing and relieving effects
CN111297737A (en) A Chinese medicinal facial mask containing Sargassum extract for promoting blood circulation and resisting aging, and its preparation method
CN116270357B (en) Rapid brightening skin care composition and application thereof
CN104352424A (en) Cordyceps militaris ingredient containing mask and preparation method thereof
CN118178298B (en) Bletilla striata extract and preparation method and application thereof
TWI802179B (en) Cactus fermentate, topical skin composition including the same with effects of moistening, redness relieving and line refining and use thereof