CN113207551A - Armillaria mellea artificial culture medium and preparation method thereof - Google Patents
Armillaria mellea artificial culture medium and preparation method thereof Download PDFInfo
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- CN113207551A CN113207551A CN202110515894.8A CN202110515894A CN113207551A CN 113207551 A CN113207551 A CN 113207551A CN 202110515894 A CN202110515894 A CN 202110515894A CN 113207551 A CN113207551 A CN 113207551A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
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Abstract
The invention belongs to the technical field of gastrodia elata symbiotic strain culture and discloses an Armillaria mellea artificial culture medium and a preparation method thereof, wherein the Armillaria mellea artificial culture medium is prepared from a base material 1: base material 2: 60 parts of auxiliary materials and base materials: 90 parts of: 15 parts of: 1 root or 90 parts: 60 parts of: 15 parts of: 1 root of the Chinese medicinal herbs. Drying the base material, the auxiliary material and the base material; respectively pulverizing the base materials and adjuvants, mixing the two base materials, adding appropriate amount of water, stirring, and packaging into culture bag; drilling a hole downwards from the center of the upper part of the culture generation to half depth, and placing one base material in the hole; filling the mixture with auxiliary materials, inoculating Armillaria mellea, and culturing. The culture medium is easy for growth and propagation of armillaria mellea rhizomes, the culture period is shortened, the risk of infectious microbe infection is reduced, and the yield of the armillaria mellea is improved. The culture medium has the advantages of wide raw material source, simplicity, easy obtainment, high safety, large and stout cultured armillaria mellea mycelium and obviously improved quality.
Description
Technical Field
The invention belongs to the technical field of gastrodia elata symbiotic strain culture, and particularly relates to an artificial culture medium for armillaria mellea and a preparation method of the artificial culture medium.
Background
At present: armillaria mellea belongs to the genus Armillaria (Trichollmatae) of the family Burleyaceae. The Armillaria mellea is a high-quality nutrient supply body required by the growth of the Gastrodia elata, and dried tubers of the Gastrodia elata are rare Chinese medicinal materials, and can normally grow and develop only by supplying nutrients to the Armillaria mellea due to the symbiotic relationship with the Armillaria mellea. At present, the gastrodia elata medicinal material is mainly from artificial cultivation. When the gastrodia elata is artificially cultivated, gastrodia elata seeds, shoestring fungi and wood sticks (providing nutrients for shoestring fungi) are cultivated together, so that the gastrodia elata can grow under the condition of continuous nutrient supply. However, the method and conditions for artificially culturing shoestring fungus of shoestring fungus and the conditions for establishing symbiotic relationship between shoestring fungus and rhizoma Gastrodiae need to be studied in depth.
The existing culture medium of halimasch has complex material, complex process, easy existence of mixed bacteria and high cost.
Through the above analysis, the problems and defects of the prior art are as follows: the existing culture medium of halimasch has complex material, complex process, easy existence of mixed bacteria and high cost.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides an Armillaria mellea artificial culture medium and a preparation method thereof.
The invention is realized in such a way that the Armillaria mellea artificial culture substrate is prepared from a base material 1: base material 2: 60 parts of auxiliary materials and base materials: 90 parts of: 15 parts of: 1 root or 90 parts: 60 parts of: 15 parts of: 1 root and the balance of water.
Further, the ratio of the base material 1 to the base material 2 in the culture medium is 60: 90 or 90: 60.
another object of the present invention is to provide a method for preparing an artificial culture substrate for armillaria mellea, comprising:
step one, drying a base material, auxiliary materials and the base material;
step two, respectively crushing the base material and the auxiliary material;
step three, according to the base material 1: base 2 ═ 60: 90 or 90: 60, mixing the two base materials, adding a proper amount of water, uniformly stirring, and filling into a culture bag (about 150 mL);
drilling a hole (phi 3cm) downwards to half depth from the center of the upper part of the culture generation, and placing one base material in the hole;
and step five, filling the mixture with auxiliary materials, wherein the dosage of the auxiliary materials is 10 percent of the sum of the volumes of the base materials 1 and 2, inoculating the armillaria mellea, and then culturing.
By combining all the technical schemes, the invention has the advantages and positive effects that: the invention has simple ingredients, simple and convenient process, difficult mixed bacteria and low cost.
The culture medium is easy for growth and reproduction of armillaria mellea rhizomorph, and reduces the risk of infectious microbe infection. The culture medium has the advantages of wide raw material source, simplicity, easy obtainment, low cost and high safety. The armillaria mellea mycelium cultured by the culture medium has a large and robust mycelium, and the quality is obviously improved.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings needed to be used in the embodiments of the present application will be briefly described below, and it is obvious that the drawings described below are only some embodiments of the present application, and it is obvious for those skilled in the art that other drawings can be obtained from the drawings without creative efforts.
FIG. 1 is a flow chart of the method for preparing an Armillaria mellea artificial culture substrate according to the embodiment of the present invention.
FIG. 2 is a flow chart of a method for performing the preparation of white clover extract as provided in the examples of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Aiming at the problems in the prior art, the invention provides an Armillaria mellea artificial culture substrate and a preparation method thereof, and the invention is described in detail with reference to the accompanying drawings.
The Armillaria mellea artificial culture medium provided by the embodiment of the invention is prepared from a base material 1: base material 2: 60 parts of auxiliary materials and base materials: 90 parts of: 15 parts of: 1 root or 90 parts: 60 parts of: 15 parts of: 1 root and the balance of water.
The culture medium provided by the embodiment of the invention is composed of a base material 1, a base material 2, auxiliary materials and a base material.
As shown in fig. 1, the preparation method of the artificial culture substrate for armillaria mellea provided by the embodiment of the invention comprises the following steps:
s101, drying the base material, the auxiliary materials and the base material;
s102, respectively crushing the base material and the auxiliary material;
s103, according to the base material 1: base 2 ═ 60: 90 or 90: 60, mixing the two base materials, adding a proper amount of water, uniformly stirring, and filling into a culture bag (about 150 mL);
s104, drilling a hole (phi 3cm) downwards to half depth from the center of the upper part of the culture generation, and placing one base material in the hole;
s105, filling the mixture with auxiliary materials, wherein the dosage of the auxiliary materials is 10% of the sum of the volumes of the base materials 1 and 2, inoculating the armillaria mellea, and then culturing.
As shown in fig. 2, in step S101, the preparation of the culture medium according to the embodiment of the present invention includes:
s201, drying the base material, the auxiliary materials and the base material in an electric heating oven at 50 ℃ to constant weight to obtain a dry raw material;
s202, respectively crushing the base material and the auxiliary materials.
The above description is only for the purpose of illustrating the present invention and the appended claims are not to be construed as limiting the scope of the invention, which is intended to cover all modifications, equivalents and improvements that are within the spirit and scope of the invention as defined by the appended claims.
Claims (7)
1. An Armillaria mellea artificial culture medium is characterized by comprising a base material 1: base material 2: 60 parts of auxiliary materials and base materials: 90 parts of: 15 parts of: 1 root or 90 parts: 60 parts of: 15 parts of: 1 root of the Chinese medicinal herbs.
2. The Armillaria mellea artificial culture medium of claim 1, wherein the culture medium comprises a base material 1, a base material 2, auxiliary materials and a base material.
3. The Armillaria mellea artificial culture medium of claim 1, wherein the ratio of the base material 1 to the base material 2 in the culture medium is 60: 90 or 90: 60.
4. a process for preparing an artificial culture medium for Armillaria mellea according to claims 1-3, which comprises:
step one, drying a base material, auxiliary materials and the base material;
step two, respectively crushing the base material and the auxiliary material;
step three, according to the base material 1: base 2 ═ 60: 90 or 90: 60, mixing the two base materials, adding a proper amount of water, uniformly stirring, and filling into a culture bag;
drilling a hole downwards from the center of the upper part of the culture generation to half depth, and placing one base material in the hole;
and step five, filling the mixture with auxiliary materials, wherein the dosage of the auxiliary materials is 10% of the sum of the volumes of the base materials 1 and 2, inoculating the armillaria mellea, and then culturing.
5. The method for preparing Armillaria mellea artificial culture substrate according to claim 4, wherein the hole is phi 3 cm.
6. The preparation method of Armillaria mellea artificial culture medium of claim 4, wherein the amount of the auxiliary materials is as follows:
the dosage of the auxiliary materials is 10 percent of the sum of the volumes of the base materials 1 and 2.
7. The method for preparing Armillaria mellea artificial culture substrate according to claim 4, wherein the volume of the culture bag is 150 mL.
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| CN202110515894.8A CN113207551A (en) | 2021-05-12 | 2021-05-12 | Armillaria mellea artificial culture medium and preparation method thereof |
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| CN202110515894.8A CN113207551A (en) | 2021-05-12 | 2021-05-12 | Armillaria mellea artificial culture medium and preparation method thereof |
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Citations (11)
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| CN104611237A (en) * | 2015-01-30 | 2015-05-13 | 麻城市天源中药材种植专业合作社 | Culture method of symbiotic amillariella mellea for gastrodia elata planting |
| CN105660143A (en) * | 2016-02-18 | 2016-06-15 | 云南省农业科学院药用植物研究所 | Production and application technology of symbiotic armillaria mellea artificial fungus stick for Gastrodia elata Bl. |
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| CN106085874A (en) * | 2016-06-23 | 2016-11-09 | 宜昌鸿升生物科技有限公司 | A kind of preparation method of Rhizoma Gastrodiae association Armillaria mellea |
| CN107439350A (en) * | 2017-08-25 | 2017-12-08 | 道真仡佬族苗族自治县联合中药材种植科技服务中心 | Armillaria mellea accreting with Rhizoma Gastrodiae breeding method |
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2021
- 2021-05-12 CN CN202110515894.8A patent/CN113207551A/en active Pending
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| JPH0662661A (en) * | 1992-08-19 | 1994-03-08 | Hokkaido Prefecture | Medium for culturing basidiomycete belonging to genus armillariella |
| JP2001314123A (en) * | 2000-05-08 | 2001-11-13 | Kiyoshi Fukuda | Mycorrhiza bed log for cultivating armillaria sinapina and method for cultivating armillaria sinapina |
| CN102742452A (en) * | 2011-04-19 | 2012-10-24 | 重庆市药物种植研究所 | Manufacture method of corncob halimasch production seeds |
| KR20130098946A (en) * | 2013-07-24 | 2013-09-05 | (사)무주천마사업단 | A method for cultivating gastrodia elata seeds |
| CN103688751A (en) * | 2013-12-12 | 2014-04-02 | 重庆市福鑫洋食用菌有限公司 | Method for preparing armillaria mellea strains by using cut logs |
| CN104611237A (en) * | 2015-01-30 | 2015-05-13 | 麻城市天源中药材种植专业合作社 | Culture method of symbiotic amillariella mellea for gastrodia elata planting |
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