KR20130098946A - A method for cultivating gastrodia elata seeds - Google Patents

Abstract

PURPOSE: A method for cultivating high quality Gastrodia elata seedlings through sexual reproduction is provided to produce a large amount of Gastrodia elata seedlings using Gastrodia elata seeds produce by sexual reproduction and to overcome atropy of Gastrodia elata caused by continuous asexual reproduction. CONSTITUTION: A method for cultivating high quality Gastrodia elata seedlings through sexual reproduction comprises the steps of: culturing commensal bacterial in a potato dextrose agar (PDA) medium at 24-26°C; inoculating a fallen leaf mixture 15-20 days after culture; inoculating Armillaria spp. and sawdust spawn into a mixture and culturing Armillaria spp. at 24-30°C for 4-5 weeks; adding the fallen leaf mixture, scattering Gastrodia elata seeds, and germinating at 23-26°C for eight weeks; moving Gastrodia elata seeds to a medium containing Armillaria spp. when the germinated Gastrodia elata seeds are grown in a length of 2-3 cm; and applying perlite, sand, and sawdust onto the medium and growing Gastrodia elata seeds in a length of 5 cm. The fallen mixture contains 85 % of fallen leaves, 7 % of wheat bran, 5 % of grain powder, and 3 % of sugar and is sterilized at 121°C for 80-100 minutes. The mixture contains sawdust, grain powder, and branch crushed material in a weight ratio of 80:10:10 and is sterilized at 121°C for 80-100 minutes.

Description

A method for cultivating gastrodia elata seeds}

The present invention relates to a method of proliferating superior follicles through sexual reproduction, and more particularly, discloses a method of proliferating superior follicles by sexual reproduction by inducing germinal seed germination using commensal bacteria.

Chunma is a perennial higher plant belonging to the Orchidaceae family, but it is a plant with reduced photosynthetic capacity. Chunma, known to be native to high altitude areas such as Korea, China and Japan, has been used as a precious herbal medicine since ancient times. Tuber stems are effective for stroke, hypertension, dizziness, and neurological diseases, come.

Since the first time it has been found that Chimma can not grow independently and coexist through symbiosis with Mulberry Mushroom, in 1995, the mulberry mushroom strain, Chumma gum 1, became available and mass cultivation became possible. However, There are rapid changes in productivity and economic losses of cultivators due to the degradation of quality and yield due to continuous silent breeding, lack of misuse of germ strains and lack of culture technology, .

One of the most effective methods to prevent the degeneration of chamai caused by continuous silent breeding is the oily breeding method using the seed of chimaese.

According to the National Forest Research Institute, the continuous breeding of the horses produces only juvenile tubers and no mature tubers.

In the case of cultivating a horse stallion or a jamae by 5 or 6 generations in a silent breeding, the mature stalk is harvested in a small and long shape, so there is no commerciality and the productivity is also lowered, resulting in a drastic decrease in productivity.

Conventional cultivation of horse chestnuts mainly uses silent breeding to cause degeneration of rice varieties, resulting in a thin and long chunmean produced, resulting in low product value.

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made in view of the above problems, and it is an object of the present invention to provide a method for mass production of high quality horse chunks through good breeding by breeding.

Means for achieving the above object of the present invention is to provide a method for cultivating germinated seeds, which comprises culturing a symbiotic germ for promoting germination of the germinated seeds, inoculating the cultured symbionts to the leaves, seeding the germinated seeds, To a culture medium in which the bacteria are cultured.

The cultivation method of the gum seeds according to the present invention not only enables the mass production of the jasmine using the seed of the gum produced by the reproductive propagation but also overcomes the degradation of the gum caused by the continuous germination.

Hereinafter, embodiments of the present invention will be described in detail.

1. Cultivation of symbionts promoting germination of seeds of ginseng:

The symbiotic bacterium used for germination of the sperm seed is selected from either white mushrooms (Mycena osmundicola) or mushrooms (Mycena KFR11212).

The symbiotic bacteria are cultured on a PDA (Potato Dextrose agar) medium at a temperature of 24 to 26 ° C.

The mixture of the leaf litter used in the PDA medium cultivated with the symbiotic bacteria was inoculated into the medium, and the ratio of the litter, bran, grain powder and sugar was 85%, 7%, 5% and 3% Oak leaves and Quercus variabilis are preferred.

In the preparation of the leaves mixture, the leaves should be cut into squares of 10-15% of the bottom width of the container and then mixed with the mixture to be a nutrient source of germination symbiosis. After sterilization at 121 ° C for 80-100 minutes, And then sealed.

In particular, PDA culture symbiotics grown at 70 ~ 80% are used when inoculating the germination symbiotic bacteria cultured in the PDA medium. Therefore, it is preferable that the culture is carried out for 15 to 20 days since the bactericidal activity is most active at this time.

2. Culturing of Chumma germ:

Mulberry fungi are preferably used, utilizing the sawdust spawn on the market. The culture vessel uses a sealed container such as plastic, and preferably has a size of 15-40 cm X 10-20 cm X 10-30 cm, which is larger than the conventional method.

The mixture is prepared by mixing sawdust, grain powder and twigs with a weight ratio of 80, 10 and 10, then sterilized at 121 ° C for 80-100 minutes and then cooled to 20 ° C or lower. .

The incubation temperature is in the range of 24-30 ° C and 4-5 week incubation is the principle.

3. Sowing seeds of seeds:

One to four sheets of flat leaves spread on the agar medium and spreading the germic symbiotic are spread on the bottom of the container and germinated at 23 to 26 ° C for 8 weeks.

During the germination process, water droplets are formed due to the nature of the agar medium.

4. Planting stage of germinated seeds:

The germinated seeds were grown in a mixture of the leaves of the above-mentioned rhizomes. When they were grown on the agar medium and branched to 2 to 3 cm in length, the seeds were transferred to the medium of No. 2 chimpanzee and the perlite, Apply 2cm to prevent water evaporation.

When grown more than 5cm, it grows in the field for 1 year and is used as a good jima.

Claims (5)

In the superior growth method through sexual reproduction,
The symbiotic fungi were cultured in a PDA (Potato Dextrose agar) medium at 24 to 26 ° C., and then inoculated with the mixture of leaves. The percentage of litter, wheat bran, grain powder and sugar was 85% %, 5% and 3%, and the leaf blend mixture is sterilized at 121 ° C. for 80 to 100 minutes. When the leaf bladder mixture is inoculated into the germicidal culture cultivated in the PDA medium, A step of culturing germs inoculated after incubation for one day;
A predetermined closed container was prepared, and a mixture obtained by mixing sawdust, grain powder and twigs with a weight ratio of 80: 10: 10 was sterilized by heating at 121 占 폚 for 80 to 100 minutes, placed in a container, Incubating the vessel, sealing the vessel, incubating the cells for 4 to 5 weeks while maintaining the temperature at 24 to 30 ° C;
Seedling seedling seeding step in which the leaf litter mixture in which the germ bacteria are spread in the agar medium is sprinkled with germinated seeds and cultured at 23 to 26 ° C for about 8 weeks; And
When the germinated cheonma seed grows 2 to 3 cm, transfer it to a medium in which cheonma bacteria are infested, and then apply pearlite, sand and sawdust to 1 to 2 cm, and grow the cheonma seed about 5 cm to grow in the open field for 1 year. Superior growth by sexual reproduction, characterized in that it comprises a step of using.
The method of claim 1,
The symbiotic bacteria excellent growth method through sexual reproduction, characterized in that to take one of the white wrinkle mushroom or the wrinkle mushroom.
The method of claim 1,
The deciduous leaves used in the deciduous mixture is a deciduous tree or a deciduous deciduous tree, and superior follicle growth method through sexual reproduction, characterized in that using a mixture thereof.
The method of claim 1,
The cheonma bacteria is a superior growth method through sexual reproduction, characterized in that the mulberry mushroom bacteria are used.
The method of claim 1,
The predetermined hermetically sealed container is a superior growth by sexual reproduction, characterized in that the width, length and height are 15-40 cm, 10-20 cm and 10-30 cm, respectively.