CN106085874A - A kind of preparation method of Rhizoma Gastrodiae association Armillaria mellea - Google Patents
A kind of preparation method of Rhizoma Gastrodiae association Armillaria mellea Download PDFInfo
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- CN106085874A CN106085874A CN201610472356.4A CN201610472356A CN106085874A CN 106085874 A CN106085874 A CN 106085874A CN 201610472356 A CN201610472356 A CN 201610472356A CN 106085874 A CN106085874 A CN 106085874A
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- armillaria mellea
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Abstract
The present invention proposes the preparation method of a kind of Rhizoma Gastrodiae association Armillaria mellea, and by the concentrate of additional proportion formula at bottleneck, Rhizomorph of Armillaria mellea can cover with whole bottleneck, improves strain utilization rate；The strain processed by which, after shoestring covers with, no matter growth early stage and later stage, seldom understands bacteria infection；This concentrate is nutritious, is equivalent to a rejuvenation in the bacterial initial species of access growth course in the early stage, improves strain purity, and late growing stage shoestring is sturdy then；Strain after this PROCESS FOR TREATMENT, survival rate reaches more than 99%, and without the strain of this PROCESS FOR TREATMENT, survival rate is about 95%.
Description
Technical field
The present invention relates to the cultivation preparation field of Armillaria mellea, particularly relate to the preparation method of a kind of Rhizoma Gastrodiae association Armillaria mellea.
Background technology
Armillaria mellea and Rhizoma Gastrodiae are symbiosis, and Rhizoma Gastrodiae growth be unable to do without the Armillaria mellea of high-quality.Generally Armillaria mellea kind producer exists
During producing Armillaria mellea, process procedure is for feeding, pour water, filling tampon, sterilizing, inoculation.Bottle after culture medium is prepared and fill
Water, directly fills tampon, the Armillaria mellea grown under the method, and bottleneck end meeting large area hydropenia, shoestring growth is thin and delicate, have impact on bacterium
Planting quality, bottleneck end strain can only be lost because growing way is weak simultaneously, and waste is big.
Summary of the invention
In view of this, the present invention proposes the preparation method of high, the sturdy Rhizoma Gastrodiae association Armillaria mellea of a kind of strain survival rate.
The technical scheme is that and be achieved in that: the preparation method of a kind of Rhizoma Gastrodiae association Armillaria mellea, including following step
Suddenly,
S1, by 60～80 parts of wood flours, 10～20 parts of Testa Tritici, 10～20 parts of Semen Maydis flour, 0.5～1.5 part of white sugar, 0.5～1.5
Part Gypsum Fibrosum, 0.5～1.5 part of potassium dihydrogen phosphate, 0.5～1.5 part of magnesium sulfate, weigh and mix homogeneously in proportion, is prepared as cultivating
Base, loads in tight mouth bottle；
S2, adding water to water content is 50～70%, and the culture medium at the bottom of guaranteeing bottle the most fully absorbs water；
S3, is deployed into concentrate by Semen Maydis flour and Testa Tritici by 1:0.8～1.2 part by weight, adds water, make water content be 50～
70%, this concentrate is loaded bottleneck by funnel, then with smashing wood compacting；
S4,90～110 DEG C of sterilizings 8～12h under normal pressure, or under 80～120kPa pressure, 110～130 DEG C of sterilizings 3～5 are little
Time, boil in a covered pot over a slow fire 2～6 hours after closing fire, the place that the culture bottle that sterilizing is good moves to aeration-drying is cooled down；
S5, is placed on superclean bench inoculation by the compost after cooling；
S6, at 20～25 DEG C, light culture 45 days, i.e. obtain Armillaria mellea kind after cleaning miscellaneous bacteria.
On the basis of above technical scheme, it is preferred that in described step S1, nutrient media components is 70 parts of wood flours, 15 portions of wheats
Bran, 15 parts of Semen Maydis flour, 1 part of white sugar, 1 part of Gypsum Fibrosum, 1 part of potassium dihydrogen phosphate, 1 part of magnesium sulfate.
On the basis of above technical scheme, it is preferred that in described step S3, Semen Maydis flour and Testa Tritici press 1:1 part by weight tune
It is made into concentrate.
On the basis of above technical scheme, it is preferred that 100 DEG C of sterilizings of normal pressure 10 hours in described step S4, or
The lower 120 DEG C of sterilizings of 100kPa pressure 4 hours, boil in a covered pot over a slow fire 4 hours after closing fire.
On the basis of above technical scheme, it is preferred that described step S6 obtain the survival rate of Armillaria mellea kind 99% with
On.
The preparation method of the Rhizoma Gastrodiae association Armillaria mellea of the present invention has the advantages that relative to prior art
(1) by the concentrate of additional proportion formula at bottleneck, and with smashing wood by reality so that culture medium is more stepped up in bottle
Real, press close to a bottle wall, after high temperature sterilize, observe bottleneck, culture medium moisturizing and be close to a bottle wall, through 25 degrees Celsius of dark trainings after inoculation
Supporting 45 days, the shoestring of growth is vigorous pure white, and bottleneck end does not exist water shortage problem, and Rhizomorph of Armillaria mellea can cover with whole bottleneck, improves
Strain utilization rate；
(2) strain processed by which, after shoestring covers with, no matter growth early stage and later stage, seldom can be infected
Miscellaneous bacteria, reason is that the culture medium covering with shoestring creates resistancing action to miscellaneous bacteria, and the lifting of survival rate helps manufacturer to reduce
Loss and management cost；
(3) this concentrate is nutritious, is equivalent to a rejuvenation, improves in the bacterial initial species of access growth course in the early stage
Strain purity, late growing stage shoestring is sturdy then；
(4) strain after this PROCESS FOR TREATMENT, survival rate reaches more than 99%, and without the strain of this PROCESS FOR TREATMENT,
Survival rate is about 95%.
Detailed description of the invention
Below in conjunction with embodiment of the present invention, technical scheme is clearly and completely described, it is clear that
Described embodiment is only a part of embodiment of the present invention rather than whole embodiments.Based in the present invention
Embodiment, the every other embodiment party that those of ordinary skill in the art are obtained under not making creative work premise
Formula, broadly falls into the scope of protection of the invention.
Embodiment 1
The preparation method of the Rhizoma Gastrodiae association Armillaria mellea of the present embodiment, comprises the following steps,
1, bottling.By 60 parts of wood flours, 10 parts of Testa Tritici, 10 parts of Semen Maydis flour, 0.5 part of white sugar, 0.5 part of Gypsum Fibrosum, 0.5 part of di(2-ethylhexyl)phosphate
Hydrogen potassium, 0.5 part of magnesium sulfate, weigh in proportion and mix homogeneously, be prepared as culture medium, load in tight mouth bottle.
2, pour water.Adding water to water content is 50%, and the culture medium at the bottom of guaranteeing bottle the most fully absorbs water.
3, concentrate is filled.Semen Maydis flour and Testa Tritici are deployed into concentrate by 1:0.8 part by weight, add water, make the water content be
50%, this concentrate is loaded bottleneck by funnel, then with smashing wood compacting.Concentrate is pressed to a bottle wall by compacting process as far as possible, fills
Concentrate is at bottleneck 1CM.Load onto tampon.
4, sterilizing.The lower 100 DEG C of sterilizing 8h of normal pressure, boil in a covered pot over a slow fire 2 hours after closing fire, the culture bottle that sterilizing is good are moved to aeration-drying
Place cools down.
5, inoculation.Treat that compost temperature is down to less than 28 DEG C, the compost after cooling is placed on superclean bench inoculation；
6, cultivate.At 20 DEG C, light culture 45 days, i.e. obtain Armillaria mellea kind after cleaning miscellaneous bacteria.Strain bottleneck portion substantially without
Hydropenia sign, it is fast that lower soil species planting tall gastrodia tuber sends out shoestring speed.
Raw Armillaria mellea prepared by the present embodiment, Rhizomorph of Armillaria mellea can cover with whole bottleneck, and pure white, sturdy, survival rate reaches
99.3%.
Embodiment 2
The preparation method of the Rhizoma Gastrodiae association Armillaria mellea of the present embodiment, comprises the following steps,
1, bottling.By 70 parts of wood flours, 15 parts of Testa Tritici, 15 parts of Semen Maydis flour, 1 part of white sugar, 1 part of Gypsum Fibrosum, 1 part of potassium dihydrogen phosphate, 1
Part magnesium sulfate, weighs in proportion and mix homogeneously, is prepared as culture medium, loads in tight mouth bottle.
2, pour water.Adding water to water content is 60%, and the culture medium at the bottom of guaranteeing bottle the most fully absorbs water.
3, concentrate is filled.By 1:1 part by weight, Semen Maydis flour and Testa Tritici being deployed into concentrate, adds water, making water content is 60%,
This concentrate is loaded bottleneck by funnel, then with smashing wood compacting.Concentrate is pressed to a bottle wall by compacting process as far as possible, fills concentrate extremely
At bottleneck 1CM.Load onto tampon.
4, sterilizing.120 DEG C of sterilizings 4 hours under 100kPa pressure, close after fire stewing 4 hours, are moved by culture bottle good for sterilizing
Place to aeration-drying cools down.
5, inoculation.Treat that compost temperature is down to less than 28 DEG C, the compost after cooling is placed on superclean bench inoculation；
6, cultivate.At 23 DEG C, light culture 45 days, i.e. obtain Armillaria mellea kind after cleaning miscellaneous bacteria.Strain bottleneck portion substantially without
Hydropenia sign, it is fast that lower soil species planting tall gastrodia tuber sends out shoestring speed.
Raw Armillaria mellea prepared by the present embodiment, Rhizomorph of Armillaria mellea can cover with whole bottleneck, and pure white, sturdy, survival rate reaches
99.5%.
Embodiment 3
The preparation method of the Rhizoma Gastrodiae association Armillaria mellea of the present embodiment, comprises the following steps,
1, bottling.By 80 parts of wood flours, 20 parts of Testa Tritici, 20 parts of Semen Maydis flour, 1.5 parts of white sugar, 1.5 parts of Gypsum Fibrosum, 1.5 parts of di(2-ethylhexyl)phosphates
Hydrogen potassium, 1.5 parts of magnesium sulfate, weigh in proportion and mix homogeneously, be prepared as culture medium, load in tight mouth bottle.
2, pour water.Adding water to water content is 70%, and the culture medium at the bottom of guaranteeing bottle the most fully absorbs water.
3, concentrate is filled.Semen Maydis flour and Testa Tritici are deployed into concentrate by 1:1.2 part by weight, add water, make the water content be
70%, this concentrate is loaded bottleneck by funnel, then with smashing wood compacting.Concentrate is pressed to a bottle wall by compacting process as far as possible, fills
Concentrate is at bottleneck 1CM.Load onto tampon.
4, sterilizing.130 DEG C of sterilizings 3 hours under 120kPa pressure, close after fire stewing 6 hours, are moved by culture bottle good for sterilizing
Place to aeration-drying cools down.
5, inoculation.Treat that compost temperature is down to less than 28 DEG C, the compost after cooling is placed on superclean bench inoculation；
6, cultivate.At 25 DEG C, light culture 45 days, i.e. obtain Armillaria mellea kind after cleaning miscellaneous bacteria.Strain bottleneck portion substantially without
Hydropenia sign, it is fast that lower soil species planting tall gastrodia tuber sends out shoestring speed.
Raw Armillaria mellea prepared by the present embodiment, Rhizomorph of Armillaria mellea can cover with whole bottleneck, and pure white, sturdy, survival rate reaches
99.4%.
The foregoing is only the better embodiment of the present invention, not in order to limit the present invention, all the present invention's
Within spirit and principle, any modification, equivalent substitution and improvement etc. made, should be included within the scope of the present invention.
Claims (5)
1. the preparation method of a Rhizoma Gastrodiae association Armillaria mellea, it is characterised in that: comprise the following steps,
S1, by 60～80 parts of wood flours, 10～20 parts of Testa Tritici, 10～20 parts of Semen Maydis flour, 0.5～1.5 part of white sugar, 0.5～1.5 part of stone
Cream, 0.5～1.5 part of potassium dihydrogen phosphate, 0.5～1.5 part of magnesium sulfate, weigh in proportion and mix homogeneously, be prepared as culture medium, dress
Enter in tight mouth bottle；
S2, adding water to water content is 50～70%, and the culture medium at the bottom of guaranteeing bottle the most fully absorbs water；
S3, is deployed into concentrate by Semen Maydis flour and Testa Tritici by 1:0.8～1.2 part by weight, adds water, and making water content is 50～70%,
This concentrate is loaded bottleneck by funnel, then with smashing wood compacting；
S4,90～110 DEG C of sterilizings 8～12h under normal pressure, or 110～130 DEG C of sterilizings 3～5 hours under 80～120kPa pressure,
Boil in a covered pot over a slow fire 2～6 hours after closing fire, the place that the culture bottle that sterilizing is good moves to aeration-drying is cooled down；
S5, is placed on superclean bench inoculation by the compost after cooling；
S6, at 20～25 DEG C, light culture 45 days, i.e. obtain Armillaria mellea kind after cleaning miscellaneous bacteria.
2. the preparation method of Rhizoma Gastrodiae association Armillaria mellea as claimed in claim 1, it is characterised in that: culture medium in described step S1
Component is 70 parts of wood flours, 15 parts of Testa Tritici, 15 parts of Semen Maydis flour, 1 part of white sugar, 1 part of Gypsum Fibrosum, 1 part of potassium dihydrogen phosphate, 1 part of magnesium sulfate.
3. the preparation method of Rhizoma Gastrodiae association Armillaria mellea as claimed in claim 1, it is characterised in that: Semen Maydis flour in described step S3
It is deployed into concentrate by 1:1 part by weight with Testa Tritici.
4. the preparation method of Rhizoma Gastrodiae association Armillaria mellea as claimed in claim 1, it is characterised in that: at normal pressure in described step S4
100 DEG C of sterilizings 10 hours, or 120 DEG C of sterilizings 4 hours under 100kPa pressure, close after fire stewing 4 hours.
5. the preparation method of Rhizoma Gastrodiae association Armillaria mellea as claimed in claim 1, it is characterised in that: described step S6 obtains honey ring
The survival rate of strain is more than 99%.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110100657A (en) * | 2019-06-03 | 2019-08-09 | 贵州织金毫米农业科技发展有限公司 | A kind of production method of Dictyophora rubrovalvata test tube stock |
| CN113207551A (en) * | 2021-05-12 | 2021-08-06 | 贵州大学 | Armillaria mellea artificial culture medium and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101946639A (en) * | 2010-10-08 | 2011-01-19 | 张光文 | Bagged gastrodia elata armillaria mellea and preparation method thereof |
| CN102342219A (en) * | 2011-08-03 | 2012-02-08 | 宜昌森源食用菌有限责任公司 | Preparation method of bottled armillaria mellea strain |
| CN102742452A (en) * | 2011-04-19 | 2012-10-24 | 重庆市药物种植研究所 | Manufacture method of corncob halimasch production seeds |
-
2016
- 2016-06-23 CN CN201610472356.4A patent/CN106085874B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101946639A (en) * | 2010-10-08 | 2011-01-19 | 张光文 | Bagged gastrodia elata armillaria mellea and preparation method thereof |
| CN102742452A (en) * | 2011-04-19 | 2012-10-24 | 重庆市药物种植研究所 | Manufacture method of corncob halimasch production seeds |
| CN102342219A (en) * | 2011-08-03 | 2012-02-08 | 宜昌森源食用菌有限责任公司 | Preparation method of bottled armillaria mellea strain |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110100657A (en) * | 2019-06-03 | 2019-08-09 | 贵州织金毫米农业科技发展有限公司 | A kind of production method of Dictyophora rubrovalvata test tube stock |
| CN113207551A (en) * | 2021-05-12 | 2021-08-06 | 贵州大学 | Armillaria mellea artificial culture medium and preparation method thereof |
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