CN1131320C - 带发光标记的低核苷酸探针的溶液中的淬火方法 - Google Patents

带发光标记的低核苷酸探针的溶液中的淬火方法 Download PDF

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CN1131320C
CN1131320C CN95121850A CN95121850A CN1131320C CN 1131320 C CN1131320 C CN 1131320C CN 95121850 A CN95121850 A CN 95121850A CN 95121850 A CN95121850 A CN 95121850A CN 1131320 C CN1131320 C CN 1131320C
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oligonucleotide
mark
nucleic acid
probe
luminous
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Expired - Lifetime
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CN95121850A
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CN1133888A (zh
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T·K·H·皮孔
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CN95121850A 1994-11-23 1995-11-23 带发光标记的低核苷酸探针的溶液中的淬火方法 Expired - Lifetime CN1131320C (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US347657 1994-11-23
US08/347,657 US5571673A (en) 1994-11-23 1994-11-23 Methods for in-solution quenching of fluorescently labeled oligonucleotide probes

Publications (2)

Publication Number Publication Date
CN1133888A CN1133888A (zh) 1996-10-23
CN1131320C true CN1131320C (zh) 2003-12-17

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CN95121850A Expired - Lifetime CN1131320C (zh) 1994-11-23 1995-11-23 带发光标记的低核苷酸探针的溶液中的淬火方法

Country Status (14)

Country Link
US (1) US5571673A (fr)
EP (1) EP0713921B1 (fr)
JP (1) JP3600333B2 (fr)
KR (1) KR100431421B1 (fr)
CN (1) CN1131320C (fr)
AT (1) ATE204333T1 (fr)
AU (1) AU700885B2 (fr)
CA (1) CA2163388C (fr)
DE (1) DE69522176T2 (fr)
DK (1) DK0713921T3 (fr)
ES (1) ES2162630T3 (fr)
NZ (1) NZ280506A (fr)
PT (1) PT713921E (fr)
RU (1) RU2149186C1 (fr)

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Also Published As

Publication number Publication date
JP3600333B2 (ja) 2004-12-15
DE69522176D1 (de) 2001-09-20
RU2149186C1 (ru) 2000-05-20
CA2163388A1 (fr) 1996-05-24
ATE204333T1 (de) 2001-09-15
ES2162630T3 (es) 2002-01-01
EP0713921B1 (fr) 2001-08-16
DK0713921T3 (da) 2001-11-12
EP0713921A3 (fr) 1996-09-11
AU700885B2 (en) 1999-01-14
KR960017858A (ko) 1996-06-17
EP0713921A2 (fr) 1996-05-29
US5571673A (en) 1996-11-05
PT713921E (pt) 2002-02-28
AU3901095A (en) 1996-05-30
KR100431421B1 (ko) 2004-12-03
DE69522176T2 (de) 2002-05-29
CN1133888A (zh) 1996-10-23
JPH08220092A (ja) 1996-08-30
CA2163388C (fr) 2008-01-15
NZ280506A (en) 1997-07-27

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