CN113080110B - Method for promoting gonad maturation of parent fish of spotted-head fish - Google Patents
Method for promoting gonad maturation of parent fish of spotted-head fish Download PDFInfo
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- CN113080110B CN113080110B CN202110347020.6A CN202110347020A CN113080110B CN 113080110 B CN113080110 B CN 113080110B CN 202110347020 A CN202110347020 A CN 202110347020A CN 113080110 B CN113080110 B CN 113080110B
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- 210000002149 gonad Anatomy 0.000 title claims abstract description 13
- 238000000034 method Methods 0.000 title claims abstract description 12
- 230000001737 promoting effect Effects 0.000 title claims abstract description 7
- 241000251468 Actinopterygii Species 0.000 title abstract description 32
- 230000035800 maturation Effects 0.000 title description 3
- 238000005286 illumination Methods 0.000 claims abstract description 60
- 230000003203 everyday effect Effects 0.000 claims abstract description 32
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 27
- 241000269821 Scombridae Species 0.000 claims description 6
- 241000238557 Decapoda Species 0.000 claims description 5
- 206010021033 Hypomenorrhoea Diseases 0.000 claims description 5
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 4
- 229910052760 oxygen Inorganic materials 0.000 claims description 4
- 239000001301 oxygen Substances 0.000 claims description 4
- 239000001963 growth medium Substances 0.000 claims description 2
- 235000015170 shellfish Nutrition 0.000 claims description 2
- 238000004140 cleaning Methods 0.000 claims 1
- 230000003247 decreasing effect Effects 0.000 abstract 1
- 238000009364 mariculture Methods 0.000 abstract 1
- 235000019688 fish Nutrition 0.000 description 27
- 210000004392 genitalia Anatomy 0.000 description 8
- 239000011148 porous material Substances 0.000 description 8
- 210000001015 abdomen Anatomy 0.000 description 6
- 238000001816 cooling Methods 0.000 description 6
- 235000013601 eggs Nutrition 0.000 description 6
- 238000009395 breeding Methods 0.000 description 5
- 230000001488 breeding effect Effects 0.000 description 5
- 241000791868 Selene orstedii Species 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 230000009467 reduction Effects 0.000 description 3
- 241001249573 Hexagrammos Species 0.000 description 2
- 241001249555 Hexagrammos otakii Species 0.000 description 2
- 241001441728 Molidae Species 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 230000002354 daily effect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000026109 gonad development Effects 0.000 description 2
- 235000020640 mackerel Nutrition 0.000 description 2
- 238000003825 pressing Methods 0.000 description 2
- 230000005070 ripening Effects 0.000 description 2
- 239000013535 sea water Substances 0.000 description 2
- 210000000582 semen Anatomy 0.000 description 2
- 241000195493 Cryptophyta Species 0.000 description 1
- 241001417941 Hexagrammidae Species 0.000 description 1
- 241001442213 Hexagrammos agrammus Species 0.000 description 1
- 241000243827 Nereis Species 0.000 description 1
- 241000276618 Perciformes Species 0.000 description 1
- 241000867416 Pseudomorpha Species 0.000 description 1
- 241001481818 Sebastes Species 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 238000010923 batch production Methods 0.000 description 1
- 230000006399 behavior Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000009182 swimming Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention relates to a method for promoting gonad maturity of parent fish of spotted-head fish, belonging to the field of mariculture. The conventional culture water temperature is reduced to 17 ℃ by 0.5 ℃ every day, the illumination period starts from the conventional 12-hour illumination and 12-hour darkness every day, the illumination time is reduced by 20 minutes and the darkness is increased by 20 minutes every day, and when the illumination period of every day is changed into the 10-hour illumination and 14-hour darkness period, the illumination period is maintained for 5 to 10 days; reducing the temperature by 0.5 ℃ to 14 ℃ every day, reducing the illumination time by 30 minutes and increasing the darkness by 30 minutes every day, and maintaining for 5-10 days when the illumination period of each day is changed into the 16-hour illumination dark period; reducing the temperature by 1 ℃ to 12 ℃ every day, and maintaining the temperature of 12 ℃ all the time; the light time per day was decreased by 60 minutes and the darkness increased by 60 minutes, and continued when the light cycle per day was changed to 6 hours of light and 18 hours of darkness.
Description
Technical Field
The invention relates to a method for promoting gonad maturity of parent fish, in particular to a method for promoting gonad maturity of spotted head fish, which is used for increasing fertilization rate and hatchability of the spotted head fish.
Background
The spotted-head fish (Hexagrammos agrammus) belongs to the sebastes pseudomorpha (Scorpaeniformes), the hexagrammodae (Hexagrammodae) and the Hexagrammos (Hexagrammos), is offshore cold-temperature bottom fish, is mainly distributed in Japan, Korean peninsula, Bohai sea, yellow sea and east sea in the northwest Pacific ocean, has obvious aggregation behaviors, is offshore cold-temperature bottom fish, is commonly mixed with Hexagrammos otakii to live around rocky rocks or in shallow sea algae areas, and is one of economic fish species with great development prospects in China sea areas. The greenling is tender in meat quality, delicious in taste and high in economic value, is deeply favored by wide consumers, is the ideal kind of cage culture in northern China like hexagrammos otakii, and is also the ideal variety for developing fishery proliferation and releasing and developing swimming fishing industry. As the natural spawning season is from autumn to winter, the temperature rise cost is not high in the artificial breeding process in the north of China, the production cost of enterprises can be greatly saved, and the method has wide popularization prospect.
At present, domestic spotted head fishes basically depend on fishing, the related research of the spotted head fishes mostly focuses on the aspects of biology and fishery resources such as growth, ingestion, nutrition and the like, and the technology for artificially breeding the spotted head fish fry is not reported. As the natural resources of the spotted-head fishes are few, the artificial breeding and fry technology is not broken through, the cage culture in the north can not be carried out, and the development of the fishing industry is limited. The technical bottleneck of artificial breeding of spotted-head fish fry is mainly that parent fish with mature gonad can not be obtained artificially, so that enough fertilized eggs can not be obtained. The reason for this is that most of the organisms in the nature lay eggs in spring with the temperature being warmed up, and the mackerel lays eggs in autumn and winter, and the traditional fish gonad maturing mode is not suitable for the mackerel.
Disclosure of Invention
Compared with the situation that the gonads of the spawning fishes in spring are promoted by temperature rise, the natural spawning season of the spotted-head fishes is from autumn to winter, and the gonad maturation presents different characteristics along with the change of temperature and illumination period.
The technical scheme adopted by the invention for solving the technical problems is as follows: a method for promoting gonad maturity of parent mackerel fish is characterized by comprising the following steps of putting mackerel fish with the age of not less than 2 years into a culture medium with the salinity of 29-31, pH: 7.8-8.1, in a culture pond with the water temperature of 20-23 ℃, the density of 0.5-2 tails/M3, the illumination intensity of 300-500 lx, the illumination period of 12 hours and the darkness of 12 hours, the running water culture, the running water keeps the dissolved oxygen above 6mg/L, the bait is any one of benthic shrimps, crabs, small-sized shellfish and clamworms, and the bottom is cleaned regularly; the method is characterized in that a, the water temperature of the first stage culture is reduced by 0.5 ℃ to 17 ℃ every day and maintained for 5-10 days, the water temperature of the second stage culture is reduced by 0.5 ℃ to 14 ℃ every day and maintained for 5-10 days, and the water temperature of the third stage culture is reduced by 1 ℃ to 12 ℃ every day and maintained for 12 ℃; b. corresponding to the reduction of the water temperature of the first stage cultivation, the illumination period starts from the conventional 12-hour illumination and 12-hour darkness every day, the illumination time is reduced by 20 minutes and the darkness is increased by 20 minutes every day, and when the illumination period of each day is changed into the 10-hour illumination and 14-hour darkness period, the illumination period is maintained for 5 to 10 days; corresponding to the reduction of the water temperature of the second stage culture, the illumination time is reduced by 30 minutes and the darkness is increased by 30 minutes every day, and when the illumination period of every day is changed into the illumination period of 8 hours and 16 hours, the illumination period is maintained for 5 to 10 days; c. corresponding to the reduction of the water temperature of the third stage culture, the daily illumination time is reduced by 60 minutes and the darkness is increased by 60 minutes, and when the daily illumination period is changed into a 6-hour illumination 18-hour darkness period, the maintenance is continued until the gonads are mature.
The invention has the beneficial effects that: the method has the advantages that the full-artificial cultivation of batch production of the spotted-head fish fries is realized, new species are added for cage culture fish in northern China, the development of the fishing industry can be facilitated through releasing, and the problems that 1) mature eggs and mature sperms cannot be obtained through artificial breeding of the spotted-head fish are solved; 2) the limitations of the traditional fish gonad maturing mode; 3) only the uncertainty of the one-factor control of temperature or photoperiod is considered.
The present invention will be further described with reference to the following examples.
Detailed Description
The first embodiment is as follows:
2-year-old parent fish, 200-230g of body weight, 1 tail/M3 of density, and culture conditions: salinity of 29-31, pH: 7.8-8.1, illumination 400lx, illumination period: 12 hours of illumination and 12 hours of darkness, culturing in running water, keeping the dissolved oxygen above 6mg/L, feeding: the benthic shrimps are matched with matched bait, the bottom is cleaned regularly, the conventional culture temperature is about 22 ℃, the first-stage cooling and light-adjusting illumination period is started for 10 months and 20 days to promote the maturity, the water temperature is reduced by 0.5 ℃ every day, the temperature is reduced to about 17 ℃ in 10 months and 29 days, the illumination time is reduced by 20 minutes and the darkness is increased by 20 minutes every day, and the 10-month 26 sunlight illumination period is a 10-hour illumination 14-hour dark illumination period; starting the second stage of cooling and adjusting the illumination period to promote ripening at 6 days in 11 months, reducing the temperature by 0.5 ℃ every day, reducing the temperature to about 14 ℃ in 11 months and 11 days in 11 months, reducing the illumination time by 30 minutes in each day, increasing the darkness by 30 minutes, and changing the illumination period of 10 months in 11 months into the illumination period of 16 hours in 8 hours and darkness; starting a third-stage cooling and light-adjusting period to promote maturity at 20 days in 11 months, reducing the temperature by 1 ℃ every day, reducing the temperature by about 12 ℃ at 21 days in 11 months, reducing the light time by 60 minutes every day, increasing the darkness by 60 minutes, changing the light period of 22 days in 11 months into 6 hours, changing the light period into 18 hours of darkness, continuously maintaining, starting taking a parent fish for examination at 1 day in 12 months, finding female fish at 5 days in 12 months, expanding and soft abdomen, red and swollen genital pores and good gonad development, wiping the fish body dry, pressing the abdomen in the direction of the genital pores, and allowing fish eggs to flow out; the male fish rubs off the seawater near the genital pore, then squeezes the abdomen towards the genital pore, and the semen flows out.
The second embodiment: 7.8-8.1, illumination 400lx, illumination period: 12 hours of illumination and 12 hours of darkness, culturing in running water, keeping the dissolved oxygen above 6mg/L, feeding: the nereis are matched with matched bait, the bottom is cleaned regularly, the conventional culture temperature is about 22 ℃, the first-stage cooling and light-adjusting illumination period is started for 10 months and 18 days to promote maturity, the water temperature is lowered by 0.5 ℃ every day, the temperature is lowered to about 17 ℃ in 10 months and 27 days, the illumination time is reduced by 20 minutes every day, the darkness is increased by 20 minutes, and the 10-month 24-day illumination period is a 10-hour illumination 14-hour dark illumination period; starting the second stage of cooling and adjusting the illumination period to promote ripening at 5 days in 11 months, reducing the temperature by 0.5 ℃ every day, reducing the temperature to about 14 ℃ in 10 days in 11 months, reducing the illumination time by 30 minutes in each day, increasing the darkness by 30 minutes, and changing the sunlight period of 9 months in 11 months into the sunlight period of 16 hours in 8 hours and darkness; starting a third-stage cooling and light-adjusting period to promote maturity at 11-18 months, reducing the temperature by 1 ℃ every day, reducing the temperature by about 12 ℃ at 11-19 months, reducing the light time by 60 minutes every day, increasing the darkness by 60 minutes, changing the light period of 11-20 months into 6-hour light, changing the light period into 18-hour darkness, continuously maintaining, starting taking a parent fish at 11-28 months for examination, finding female fish at 2 months at 12-2 days, expanding and soft abdomen, red and swollen genital pores and good gonad development, wiping the fish body dry, pressing the abdomen in the direction of the genital pores, and allowing fish eggs to flow out; the male fish rubs off the seawater near the genital pore, then squeezes the abdomen towards the genital pore, and the semen flows out.
Claims (1)
1. A method for promoting gonad maturity of parent mackerel fish is characterized by comprising the following steps of putting mackerel fish with the age of not less than 2 years into a culture medium with the salinity of 29-31, pH: 7.8-8.1, water temperature of 20-23 ℃ and density of 0.5-2 tails/M3The illumination intensity is 300-500 lx, the illumination period is 12 hours, the illumination is 12 hours and the illumination is dark, the culture is carried out in running water, the dissolved oxygen is kept above 6mg/L in the running water, and the bait is addedMatching any one of benthic shrimps, crabs, small-sized shellfish and clamworms with a matched bait, and periodically cleaning the bottom; the method is characterized in that:
a. the culture water temperature is reduced by 0.5 ℃ every day until the culture water temperature is reduced to 17 ℃, meanwhile, the illumination period starts from the conventional 12-hour illumination and 12-hour darkness every day, the illumination time is reduced by 20 minutes and the darkness is increased by 20 minutes every day until the illumination period is changed into the 10-hour illumination and 14-hour darkness period every day, and the culture water temperature is maintained for 5-10 days;
b. the culture water temperature is reduced by 0.5 ℃ every day until the culture water temperature is reduced to 14 ℃, meanwhile, the illumination time is reduced by 30 minutes every day, the darkness is increased by 30 minutes until the illumination time is changed to 8 hours, and the culture water temperature is maintained for 5-10 days when the dark light period is changed to 16 hours;
c. the culture water temperature is reduced by 1 ℃ every day until the culture water temperature is reduced to 12 ℃, and the culture water temperature of 12 ℃ is maintained all the time, meanwhile, the illumination time is reduced by 60 minutes every day, the darkness is increased by 60 minutes, and the culture water temperature is maintained continuously until the period of 6-hour illumination and 18-hour darkness is changed.
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| CN202110347020.6A CN113080110B (en) | 2021-03-31 | 2021-03-31 | Method for promoting gonad maturation of parent fish of spotted-head fish |
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| CN202110347020.6A CN113080110B (en) | 2021-03-31 | 2021-03-31 | Method for promoting gonad maturation of parent fish of spotted-head fish |
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| CN114128663B (en) * | 2021-11-24 | 2023-02-10 | 大连海洋大学 | Thermo-optical regulation and control method for anti-season propagation of parent fish of takifugu rubripes and application of thermo-optical regulation and control method |
| CN115443930B (en) * | 2022-08-31 | 2023-09-05 | 渤海水产育种(海南)有限公司 | Parent shrimp cultivation method for litopenaeus vannamei with stable spawning period |
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| KR20110106172A (en) * | 2010-03-22 | 2011-09-28 | 한국해양연구원 | Identifying method of marine fishes in southern sea of korea, polynucleotide probe, dna chip and kit for identifying the same |
| CN105075947A (en) * | 2015-08-26 | 2015-11-25 | 山东省海洋生物研究院 | Hexagrammos otakii Jordan et Starks seedling intermediate cultivation method |
| CN105638525A (en) * | 2016-01-06 | 2016-06-08 | 山东东方海洋科技股份有限公司 | Closed type circulating water breeding method of hexagrammos otakii |
| CN111248119A (en) * | 2020-03-25 | 2020-06-09 | 山东省海洋生物研究院 | Artificial insemination device and artificial insemination method for spotted-head fish |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US20100037330A1 (en) * | 2008-06-11 | 2010-02-11 | Kannika Siripattarapravat | Efficient Somatic Cell Nuclear Transfer In Fish |
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| CN102077802B (en) * | 2010-12-31 | 2012-09-05 | 烟台大学 | Parent fish breeding and hatching method for greenlings |
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| CN105325751B (en) * | 2015-09-22 | 2016-10-19 | 山东省海洋生物研究院 | A kind of greenling parent fish accelerating bait and preparation method thereof |
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- 2021-03-31 CN CN202110347020.6A patent/CN113080110B/en active Active
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| KR20110106172A (en) * | 2010-03-22 | 2011-09-28 | 한국해양연구원 | Identifying method of marine fishes in southern sea of korea, polynucleotide probe, dna chip and kit for identifying the same |
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| CN105638525A (en) * | 2016-01-06 | 2016-06-08 | 山东东方海洋科技股份有限公司 | Closed type circulating water breeding method of hexagrammos otakii |
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