CN112899354A - 直接核酸扩增试剂盒、试剂和方法 - Google Patents

直接核酸扩增试剂盒、试剂和方法 Download PDF

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Publication number
CN112899354A
CN112899354A CN202110196732.2A CN202110196732A CN112899354A CN 112899354 A CN112899354 A CN 112899354A CN 202110196732 A CN202110196732 A CN 202110196732A CN 112899354 A CN112899354 A CN 112899354A
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China
Prior art keywords
nucleic acid
amplification
reagent
cyclodextrin
acid amplification
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Pending
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CN202110196732.2A
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English (en)
Chinese (zh)
Inventor
J.K.霍尔顿
P.J.塔特内尔
K.L.拉梅顿
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Global Life Sciences Solutions Operations UK Ltd
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GE Healthcare UK Ltd
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Publication of CN112899354A publication Critical patent/CN112899354A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/125—Specific component of sample, medium or buffer

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
CN202110196732.2A 2012-10-24 2013-10-23 直接核酸扩增试剂盒、试剂和方法 Pending CN112899354A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GB1219137.5 2012-10-24
GB201219137A GB201219137D0 (en) 2012-10-24 2012-10-24 Direct nucleic acid amplification kit, reagent and method
US13/799800 2013-03-13
US13/799,800 US20140113294A1 (en) 2012-10-24 2013-03-13 Direct nucleic acid amplification kit, reagent and method
CN201380055445.3A CN104736726A (zh) 2012-10-24 2013-10-23 直接核酸扩增试剂盒、试剂和方法

Related Parent Applications (1)

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CN201380055445.3A Division CN104736726A (zh) 2012-10-24 2013-10-23 直接核酸扩增试剂盒、试剂和方法

Publications (1)

Publication Number Publication Date
CN112899354A true CN112899354A (zh) 2021-06-04

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CN201380055445.3A Pending CN104736726A (zh) 2012-10-24 2013-10-23 直接核酸扩增试剂盒、试剂和方法
CN202110196732.2A Pending CN112899354A (zh) 2012-10-24 2013-10-23 直接核酸扩增试剂盒、试剂和方法

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CN201380055445.3A Pending CN104736726A (zh) 2012-10-24 2013-10-23 直接核酸扩增试剂盒、试剂和方法

Country Status (7)

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US (2) US20140113294A1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
EP (1) EP2912191B1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
JP (2) JP2015533507A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
CN (2) CN104736726A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
GB (1) GB201219137D0 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
IN (1) IN2015DN02793A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
WO (1) WO2014064169A1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)

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GB201219137D0 (en) 2012-10-24 2012-12-05 Ge Healthcare Uk Ltd Direct nucleic acid amplification kit, reagent and method
JP5904153B2 (ja) * 2013-03-29 2016-04-13 ソニー株式会社 核酸増幅反応用試料の調製方法、核酸増幅方法、固相状核酸増幅反応用試薬及びマイクロチップ
CN106715706B (zh) 2014-09-30 2022-08-09 环球生命科技咨询美国有限责任公司 直接从未纯化的生物样本分析核酸的方法
JP2018506020A (ja) * 2014-12-18 2018-03-01 ジーイー・ヘルスケア・ユーケイ・リミテッド 核酸増幅と組合せたイムノアッセイによる固体支持体上の被検体検出
US10858694B2 (en) 2014-12-23 2020-12-08 Global Life Sciences Solutions Operations UK Ltd Methods and reagents for reverse-transcription polymerase chain reaction
WO2016106111A1 (en) 2014-12-23 2016-06-30 Ge Healthcare Uk Limited Methods and reagents for reverse-transcription polymerase chain reaction
US10822645B1 (en) 2014-12-23 2020-11-03 Global Life Sciences Solutions Operations UK Ltd Methods and reagents for reverse-transcription polymerase chain reaction
GB201515355D0 (en) * 2015-08-28 2015-10-14 Ge Healthcare Ltd A method and kit for analyte detection
US20180320227A1 (en) * 2015-11-18 2018-11-08 Japan Agency For Marine-Earth Science And Technology Method for quantifying target nucleic acid and kit therefor
GB201602880D0 (en) 2016-02-18 2016-04-06 Ge Healthcare Uk Ltd Method and composition for biomolecule stabilization
US9617587B1 (en) 2016-04-04 2017-04-11 Nat Diagnostics, Inc. Isothermal amplification components and processes
CN106755414B (zh) * 2016-12-23 2020-09-01 宁波海尔施基因科技有限公司 一种检测dna遗传标记的方法
CA3059977C (en) * 2017-05-19 2024-02-13 Gen-Probe Incorporated Dried compositions containing flap endonuclease
CA3155871A1 (en) * 2017-07-10 2019-01-17 Gen-Probe Incorporated Analytical systems and methods for nucleic acid amplification using sample assigning parameters
CN115279901A (zh) * 2020-03-11 2022-11-01 花王株式会社 来自皮肤表面脂质的rna的制备方法
CN111593112A (zh) * 2020-05-12 2020-08-28 深圳市星蝶科技有限公司 一种用于检测β-地中海贫血的PCR试剂及试剂盒
US20250154607A1 (en) * 2021-03-19 2025-05-15 Becton, Dickinson And Company Isothermal amplification of pathogens
CN114317700B (zh) * 2021-12-28 2024-01-30 无锡科智达科技有限公司 荧光pcr试剂冻干保护剂、冻干方法及应用
WO2025049920A1 (en) * 2023-09-01 2025-03-06 Biomeme, Inc. Methods and compositions for sample processing

Citations (5)

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US5705345A (en) * 1991-01-10 1998-01-06 Amersham International Plc Methods and kits for preparing nucleic acids using cyclodextrin
CN101516337A (zh) * 2006-09-18 2009-08-26 通用电气医疗集团生物科学公司 用玻璃覆盖的生物学试剂的制备
WO2010002938A2 (en) * 2008-06-30 2010-01-07 Life Technologies Corporation Method for direct amplification from crude nucleic acid samples
CN102018672A (zh) * 2010-11-29 2011-04-20 广州朗圣药业有限公司 一种水溶性药物的脂质体冻干组合物及其制备方法
CN102317472A (zh) * 2008-12-12 2012-01-11 欧洲基因技术股份有限公司 使用环糊精改进核酸扩增反应的特异性、灵敏度和产率

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US5565318A (en) * 1994-09-02 1996-10-15 Pharmacia Biotech, Inc. Room temperature stable reagent semi-spheres
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JP2005532827A (ja) * 2002-07-12 2005-11-04 ブリティッシュ・バイオセル・インターナショナル・リミテッド 側方フローアッセイ用のデバイスおよびその方法
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US8153401B2 (en) * 2007-05-17 2012-04-10 Applied Biosystems, Llc Method for direct amplification from crude nucleic acid samples
GB201219137D0 (en) 2012-10-24 2012-12-05 Ge Healthcare Uk Ltd Direct nucleic acid amplification kit, reagent and method
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5705345A (en) * 1991-01-10 1998-01-06 Amersham International Plc Methods and kits for preparing nucleic acids using cyclodextrin
CN101516337A (zh) * 2006-09-18 2009-08-26 通用电气医疗集团生物科学公司 用玻璃覆盖的生物学试剂的制备
WO2010002938A2 (en) * 2008-06-30 2010-01-07 Life Technologies Corporation Method for direct amplification from crude nucleic acid samples
JP2011526788A (ja) * 2008-06-30 2011-10-20 ライフ テクノロジーズ コーポレーション 粗製核酸サンプルからの直接増幅のための方法
CN102317472A (zh) * 2008-12-12 2012-01-11 欧洲基因技术股份有限公司 使用环糊精改进核酸扩增反应的特异性、灵敏度和产率
CN102018672A (zh) * 2010-11-29 2011-04-20 广州朗圣药业有限公司 一种水溶性药物的脂质体冻干组合物及其制备方法

Also Published As

Publication number Publication date
CN104736726A (zh) 2015-06-24
US20170152546A1 (en) 2017-06-01
JP7009011B2 (ja) 2022-02-10
WO2014064169A1 (en) 2014-05-01
JP2018183156A (ja) 2018-11-22
GB201219137D0 (en) 2012-12-05
IN2015DN02793A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) 2015-09-04
EP2912191A1 (en) 2015-09-02
EP2912191B1 (en) 2019-09-04
US20140113294A1 (en) 2014-04-24
US10907201B2 (en) 2021-02-02
JP2015533507A (ja) 2015-11-26

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