CN112753495A

CN112753495A - Pteridium aquilinum bulbil propagation method

Info

Publication number: CN112753495A
Application number: CN202110001224.4A
Authority: CN
Inventors: 陈小红; 郭泽娟; 陈俐洁; 陈浩杰; 宋子衿; 李曜汐
Original assignee: Sichuan Agricultural University
Current assignee: Sichuan Agricultural University
Priority date: 2021-01-04
Filing date: 2021-01-04
Publication date: 2021-05-07

Abstract

The invention provides a method for propagating bulbil buds of ophiocarpium incarnatum, which comprises the following steps: mature bulbels are selected and sowed in moist habitat soil or nutrient soil with loose soil and good drainage. Covering soil mainly by broadcasting or covering by moss; placing the seeds in an incubator with the culture conditions of illumination intensity of 400lux, illumination time of 7h and temperature of 18-23 ℃; watering once a day, and ventilating every 2-3 days. By using the cultivation method, after two different kinds of soil cultivation, the bulbil starts to germinate after 10-15 days, the top of the bulbil starts to form the fist bud after 20-25 days, and the fist bud gradually grows to form small leaves and is robust after 30-45 days, so that the expected effect is achieved.

Description

Pteridium aquilinum bulbil propagation method

Technical Field

The invention belongs to the technical field of pteridophyte propagation and cultivation, and particularly relates to a pteridophyte bulbil propagation method.

Background

Pteridium aquilinum (Cystoathyrium chinense) is a rare fern plant of the genus of Athyriaceae (Athyriaceae), and is a first-class important protection plant of China. At present, the pteris ophioides is only found in Erlangshan and Mesempervirens in Yaan, the total number of plants is less than 100, the number of wild plants is extremely small, the distribution area is extremely narrow, and the natural updating capability is extremely weak. In 2018, when the special investigation of the plant resources of the ophiocordus glabripes is carried out, the fact that some papillae exist on the back of the phyllopodium glabripes, the papillae are located at the top of the lateral vena cava of the phyllopodium and grow in the same period as the sporangium in the middle of the lateral vena cava, the root system of the mature spores can be seen finally, and new juvenile sporophytes are found in the primordial environment, which shows that the pteropodium glabripes has the possibility of bulbar propagation besides spore propagation. Since the pteridium aquilinum is newly discovered, related departments perform certain plant rescue plans such as ecological protection and plant breeding measures, but the substantial progress is not achieved, especially the breakthrough is not achieved in the aspect of plant breeding, and the artificial spore propagation technology is not successful so far. Sichuan is used as the only distribution area of the pteridium aquilinum, how to expand the plant quantity and the population area, and how to carry out rescue protection on the pteridium aquilinum is very slow, so the research on the field rescue and breeding technology of the pteridium aquilinum is urgently needed to be carried out.

At present, the pteris vittata is only found in the Erlangshan and the Emei mountain, and has the problems of difficult natural updating, slow propagation speed, fragile ecological environment and the like. Through the breeding research of the pteridium aquilinum for a plurality of times by relevant departments, no breakthrough progress is made on the aspect of spore breeding. The invention carries out propagation culture through the bulbil for the first time so as to provide certain help for the propagation work of the ophiocarpium.

Disclosure of Invention

The invention provides a method for breeding ophiocordum latinosum, which aims to improve the species quantity of the ophiocordum latinosum by artificially cultivating the bulbil generated by the leaf back of the ophiocordum latinosum.

In order to achieve the purpose, the invention adopts the following technical scheme:

a method for reproducing bulbil buds of ophiocarpium incarnatum comprises the following steps:

s1: selecting mature bulbils, sowing the bulbils in moist habitat soil or nutrient soil with loose soil and good drainage, and covering soil with broadcast application as a main part or with moss;

s2: and (3) putting the sowed nutrient soil into an incubator, watering every day, and ventilating.

Further, in the step S2, the culture conditions in the incubator are 400lux of light intensity, 7h of light time and 18-23 ℃.

Further, in the step S1, the mature bulblets are collected generally in 8-9 months.

Further, in the step S1, the nutrient soil is prepared from sand: 1 part of loam: 1 in sandy loam.

Further, in the step S2, the water is applied once a day, and the air is ventilated every 2 to 3 days.

Furthermore, each watering is mainly carried out, and the water is slowly sprayed along the periphery of the plant; in the seedling period, the fertilizer can be directly sprayed; the watering amount is preferably 60 to 80 percent of soil humidity; the watering time follows the set light time of the incubator, and watering is generally carried out during dark culture.

Furthermore, the ventilation is carried out at night under the condition that the indoor is completely dark, and the incubator is opened for 10-12 hours generally.

Further, in the step S1, covering soil to ensure that the morphological lower end of the bulbil is contacted with soil or moss; the thickness of the covering soil is about 0.1-0.2cm, and a layer of sphagna cannot be excessively thick or directly laid on the bulbils.

The invention has the following beneficial effects:

by using the cultivation method, after two different kinds of soil cultivation, the bulbil starts to germinate after 10-15 days, the top of the bulbil starts to form the fist bud after 20-25 days, and the fist bud gradually grows to form small leaves and is robust after 30-45 days, so that the expected effect is achieved.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are a part of the embodiments of the present invention, but not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

(1) selecting mature bulbils, sowing the bulbils in moist habitat soil or nutrient soil with loose soil and good drainage, and covering soil with broadcast application as a main part or with moss;

(2) and (3) putting the sowed nutrient soil into an incubator, watering every day, and ventilating.

In this example, in the step (2), the culture conditions in the incubator are 400lux of light intensity, 7h of light time and 18-23 ℃.

In this embodiment, in step (1), the mature bulblets are collected, typically in months 8-9.

In this embodiment, in the step (1), the nutrient soil is prepared from sand soil: 1 part of loam: 1 in sandy loam.

In this embodiment, in the step (2), watering is performed once a day, and ventilation is performed every 2 to 3 days; spraying is mainly carried out in each watering, and the water is slowly sprayed along the periphery of the plant; in the seedling period, the fertilizer can be directly sprayed; the watering amount is preferably 60 to 80 percent of soil humidity; watering time follows the set illumination time of the incubator, and watering is generally carried out during dark culture; ventilating, opening the incubator at night under the condition of ensuring that the room is completely dark, wherein the time is generally 10-12 h.

In this embodiment, in the step (1), the soil is covered to ensure that the morphological lower end of the bulbil is contacted with the soil or moss; the thickness of the covering soil is about 0.1-0.2cm, and a layer of sphagna cannot be excessively thick or directly laid on the bulbils.

Example 1

10 mature bulblets (the tops of the bulblets are all protruded) are collected from the primordial habitat and cultivated by the method. Cultivating in native soil: sowing the bulbils in moist habitat soil, and covering soil mainly by broadcasting or by covering moss; placing the seeds in an incubator with the culture conditions of illumination intensity of 400lux, illumination time of 7h and temperature of 18-23 ℃; watering once a day, and ventilating every 2-3 days.

In the embodiment, the specific scheme is as follows: spraying is mainly carried out in each watering, and the water is slowly sprayed along the periphery of the plant; in the seedling period, the fertilizer can be directly sprayed; the watering amount is preferably 60 to 80 percent of soil humidity; the watering time follows the set light time of the incubator, and watering is generally carried out during dark culture.

In the embodiment, the specific scheme is as follows: ventilating every 2-3 days, and opening the incubator at night for 10-12h under the condition of ensuring that the room is completely dark.

In the embodiment, the specific scheme is as follows: covering soil to ensure that the morphological lower end of the bulbil is contacted with soil or moss; the thickness of the covering soil is about 0.1-0.2cm, and a layer of moss cannot be laid on the bulbil or directly.

As a result: under the method, the bulbule starts to germinate after 10 to 15 days, the top of the bulbule starts to form a fist bud after 20 to 25 days, and the fist bud gradually grows to form small leaves and is robust after 30 to 45 days, so that the expected effect is achieved.

Example 2

10 mature bulblets (the tops of the bulblets are all raised) are collected from the primordial habitat, and the cultivation is carried out by applying the method of the invention: and (3) cultivating in a cultivation medium: sowing the bulbels in moist nutrient soil with loose soil and good drainage, and covering soil mainly by broadcasting or by moss; placing the seeds in an incubator with the culture conditions of illumination intensity of 1000px, illumination time of 7h and temperature of 18-23 ℃; watering once a day, and ventilating every 2-3 days.

In the embodiment, the specific scheme is as follows: the nutrient soil is prepared from sandy soil and loam 1: 1 in sandy loam.

In the embodiment, the specific scheme is as follows: covering soil to ensure that the morphological lower end of the bulbil is contacted with soil or water moss; the thickness of the covering soil is about 0.1-0.2cm, and a layer of moss cannot be laid on the bulbil or directly.

Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments or portions thereof without departing from the spirit and scope of the invention.

Claims

1. A method for reproducing bulbil buds of ophiocarpium incanus is characterized by comprising the following steps:

2. The method for propagating bulbus bud of Pteris folioides according to claim 1, wherein in the step S2, the culture conditions in the incubator are 400lux of light intensity, 7h of light time and 18-23 ℃.

3. The method for propagating bulbil of ophiocordyceps nitidus as claimed in claim 1, wherein in step S1, mature bulbil is collected generally in 8-9 months.

4. The method for propagating bulbil of pteridium aquilinum according to claim 1, wherein in the step S1, the nutrient soil is prepared from sand soil: 1 part of loam: 1 in sandy loam.

5. The method for propagating bulbil of ophiocordyceps nitidus as claimed in claim 1, wherein in the step S2, watering is performed once a day, and ventilation is performed every 2-3 days.

6. The method for propagating bulbil of Pteridium aquilinum according to claim 5, wherein each watering is mainly carried out by spraying, and the spraying is carried out slowly along the periphery of the plant; in the seedling period, the fertilizer can be directly sprayed; the watering amount is preferably 60 to 80 percent of soil humidity; the watering time follows the set light time of the incubator, and watering is generally carried out during dark culture.

7. The method for propagating bulbophyllum radiatum Linn according to claim 5, wherein the cultivation box is opened at night to ensure the room is completely dark, and the time is generally 10-12 h.

8. The method for propagating bulbophyllum japonicum according to claim 1, wherein in step S1, the soil is covered to ensure that the morphological lower end of bulbophyllum is in contact with soil or moss; the thickness of the covering soil is about 0.1-0.2cm, and a layer of sphagna cannot be excessively thick or directly laid on the bulbils.