CN112753474A - Taxus chinensis cutting cultivation method and application thereof - Google Patents

Taxus chinensis cutting cultivation method and application thereof Download PDF

Info

Publication number
CN112753474A
CN112753474A CN202110102942.0A CN202110102942A CN112753474A CN 112753474 A CN112753474 A CN 112753474A CN 202110102942 A CN202110102942 A CN 202110102942A CN 112753474 A CN112753474 A CN 112753474A
Authority
CN
China
Prior art keywords
cutting
parts
seedlings
taxus chinensis
cultivation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202110102942.0A
Other languages
Chinese (zh)
Inventor
姚炳矾
黎德科
潘杰
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Libo Huangjiang River National Wetland Park Management Station
Original Assignee
Libo Huangjiang River National Wetland Park Management Station
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Libo Huangjiang River National Wetland Park Management Station filed Critical Libo Huangjiang River National Wetland Park Management Station
Priority to CN202110102942.0A priority Critical patent/CN112753474A/en
Publication of CN112753474A publication Critical patent/CN112753474A/en
Pending legal-status Critical Current

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005Cultivation methods
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G2/00Vegetative propagation
    • A01G2/10Vegetative propagation by means of cuttings
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/10Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/10Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
    • A01G24/12Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/08Oxygen or sulfur directly attached to an aromatic ring system
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/08Oxygen or sulfur directly attached to an aromatic ring system
    • A01N31/16Oxygen or sulfur directly attached to an aromatic ring system with two or more oxygen or sulfur atoms directly attached to the same aromatic ring system
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/10Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/34Nitriles
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/36Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
    • A01N43/38Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings

Abstract

The invention provides a taxus chinensis cuttage cultivation method and application thereof, and relates to the technical field of plant cuttage cultivation. The cutting cultivation method of the taxus chinensis comprises the following steps: firstly, selecting proper cutting seedlings in proper cutting time, preparing rooting liquid required by cutting, soaking the treated cutting seedlings in self-made rooting liquid for soaking treatment, preparing a matrix required by cutting cultivation, cultivating the treated cutting seedlings in the matrix, watering and fertilizing properly, and transplanting the survival Chinese yew seedlings cultivated in the matrix after one month. According to the invention, the rooting solution and the cutting medium are self-prepared, so that the survival rate of the cutting cultivation of the taxus chinensis is higher, and the seedling growth in the later period is more robust.

Description

Taxus chinensis cutting cultivation method and application thereof
Technical Field
The invention relates to the technical field of plant cuttage cultivation, in particular to a taxus chinensis cuttage cultivation method and application thereof.
Background
Taxus chinensis belongs to shallow root plants, has unobvious main roots and developed lateral roots, is a natural rare anti-cancer plant which is known to be endangered to extinct in the world, is an ancient wiggle tree species which is left over by the fourth glacier, and has a history of 250 ten thousand years on the earth. Because the growth speed of the taxus chinensis is slow and the regeneration capability is poor under natural conditions, a large-scale taxus chinensis raw material forest base, evergreen trees or shrubs have not been formed in the world for a long time; the small branches are irregularly grown, most or few bud scales lodged at the base part drop off; the winter bud scales are arranged in a shingled shape, and the longitudinal ridges on the back are obvious or not obvious. The leaf strips are spirally grown, the base parts are twisted and arranged into two rows, the two rows are straight or sickle-shaped, the two rows grow downwards, the upper part of the middle vein is raised, the lower part of the middle vein is provided with two light gray, gray green or light yellow stomatal bands, and no resin channel exists in the leaves. Male and female heterozygote, the unigenetic axillae of the ball flower; the male ball flower is spherical and has a stalk, the base part of the male ball flower is provided with bracts arranged in a covering tile shape, 6-14 stamens are arranged in a shield shape, 4-9 anthers are arranged in a radiation manner; the female ball flower has no stem, the base part has a plurality of bracts arranged in a covering tile shape, the upper ends of 2-3 pairs of bracts are crossed and oppositely arranged, ovules are upright and singly grow on bract armpits at the top end of the short axis on the upper part of the total flower axis, the base part is supported by a disc-shaped pearl support, and the pearl support develops into a fleshy, cupped and red aril after fertilization. The seeds are nut-shaped, mature in the year, grow in the pseudoseed coat with cup-shaped meat quality, and are sparsely grown on a seed tray (namely a bead tray which does not develop into the meat pseudoseed coat) with a disk shape close to membranous texture, the hilum is obvious, and the meat pseudoseed coat is red in color and has short stalks or almost no stalks when mature; 2 cotyledons, which come out of the soil when sprouting.
In order to promote the propagation and growth of the Taxus chinensis, a treasure plant, namely Taxus chinensis, needs to be subjected to cuttage breeding, but in the prior art, the cuttage breeding efficiency of Taxus chinensis is low, and the survival rate is not high.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides a taxus chinensis cuttage cultivation method and application thereof, and solves the problem of low cuttage survival rate of taxus chinensis.
(II) technical scheme
In order to achieve the purpose, the invention is realized by the following technical scheme: a cutting cultivation method of Chinese yew comprises the following steps: firstly, selecting proper cutting seedlings in proper cutting time, preparing rooting liquid required by cutting, soaking the treated cutting seedlings in self-made rooting liquid for soaking treatment, preparing a matrix required by cutting cultivation, cultivating the treated cutting seedlings in the matrix, watering and fertilizing properly, and transplanting the survival Chinese yew seedlings cultivated in the matrix after one month.
Preferably, the cutting cultivation time is 3, 7 and 11 months per year, an annual branch is selected from healthy and strong yew trees as a cutting shoot, the yew cutting shoot is cut into small sections of about 15 cm, and the top of each small section is provided with a plurality of leaves to prepare a cutting seedling.
Preferably, the rooting solution is weighed according to the following formula: c12H10O220-30 parts of C10H9NO215-20 parts of C12H13NO28-9 parts of C12H10O310-12 parts of phenol compound, 2-3 parts of phenol compound, 1000 parts of water and 1200 parts of C12H10O2、C10H9NO2、C12H13NO2、C12H10O3The phenol compound and water are fully mixed and stirred to obtain rooting liquid.
Preferably, the matrix required by the cutting cultivation comprises 15-20 parts of plant ash, 700 parts of crude river sand and 15-20 parts of farmyard manure, and the specific preparation method comprises the following steps of separating the crude river sand into A parts and B parts, wherein the A parts account for one fifth of the total amount of the crude river sand, the B parts account for four fifths of the total amount of the crude river sand, soaking the crude river sand of the A parts in a rooting solution, fully stirring and mixing the crude river sand of the A parts and the crude river sand of the B parts to obtain a matrix sand base, adding the plant ash and the farmyard manure into the matrix sand base, fully mixing and stirring to obtain a cultivation matrix, adding water to enable the matrix to be gripped by hands, tightly gripping the matrix, preventing water from dripping at finger joints, and preventing the matrix from scattering after the hands are loosened.
Preferably, the specific cuttage method of the taxus chinensis is as follows:
checking the cutting seedlings to ensure that the cutting seedlings have activity and are healthy and strong, and soaking the qualified cutting seedlings in a rooting solution for 14-17 hours;
inserting the cutting seedlings soaked with the rooting solution into the prepared culture medium, wherein the insertion depth is four to five centimeters, placing the plants in a cool and ventilated place after the cutting, spraying a certain amount of water by using a spray can, and moving the plants to a light-scattering place for maintenance after one week;
and step three, cutting the yew seedlings cut in the step two for one month, digging the cut seedlings for inspection, and taking the healthy and alive yew seedlings for transplanting and planting.
The application of the taxus chinensis cutting cultivation method of any one of claims 1 to 5, which is applied to the technical field of plant cutting propagation.
(III) advantageous effects
The invention provides a taxus chinensis cutting cultivation method and application thereof. The method has the following beneficial effects:
according to the invention, the rooting solution and the cutting medium are self-prepared, so that the survival rate of the cutting cultivation of the taxus chinensis is higher, and the seedling growth in the later period is more robust.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The first embodiment is as follows:
the embodiment of the invention provides a taxus chinensis cutting cultivation method, which comprises the following steps: firstly, selecting proper cutting seedlings in proper cutting time, preparing rooting liquid required by cutting, soaking the treated cutting seedlings in self-made rooting liquid for soaking treatment, preparing a matrix required by cutting cultivation, cultivating the treated cutting seedlings in the matrix, watering and fertilizing properly, and transplanting the survival Chinese yew seedlings cultivated in the matrix after one month.
The cutting cultivation time is selected in 3, 7 and 11 months per year, an annual branch is selected from a healthy and strong yew tree as a cutting shoot, the yew cutting shoot is cut into small sections of about 15 cm, and the top of each small section is provided with a plurality of leaves to prepare a cutting seedling. Weighing the raw materials of the rooting solution in parts by weight according to the following formula: c12H10O220 parts of C10H9NO215 parts of C12H13NO228 parts of C12H10O310 parts of phenol compound, 2 parts of water and 1000 parts of C12H10O2、C10H9NO2、C12H13NO2、C12H10O3The phenol compound and water are fully mixed and stirred to obtain rooting liquid.
The substrate required by cutting cultivation comprises 15 parts of plant ash, 500 parts of crude river sand and 15 parts of farmyard manure, and the specific preparation method comprises the following steps of separating the crude river sand into A parts and B parts, wherein the A parts account for one fifth of the total amount of the crude river sand, the B parts account for four fifths of the total amount of the crude river sand, soaking the A parts of the crude river sand in rooting liquid, fully stirring and mixing the A parts of the crude river sand and the B parts of the crude river sand to obtain a substrate sand base, adding the plant ash and the farmyard manure into the substrate sand base, fully mixing and stirring to obtain a cultivation substrate, adding water to enable a user to grasp one substrate by hand, grasping the substrate tightly, preventing water from dripping at finger joints, and preventing the substrate from scattering after the user loosens the hand.
The specific cuttage method of the taxus chinensis comprises the following steps:
checking the cutting seedlings to ensure that the cutting seedlings have activity and are healthy and strong, and soaking the qualified cutting seedlings in a rooting solution for 14-17 hours;
inserting the cutting seedlings soaked with the rooting solution into the prepared culture medium, wherein the insertion depth is four to five centimeters, placing the plants in a cool and ventilated place after the cutting, spraying a certain amount of water by using a spray can, and moving the plants to a light-scattering place for maintenance after one week;
and step three, cutting the yew seedlings cut in the step two for one month, digging the cut seedlings for inspection, and taking the healthy and alive yew seedlings for transplanting and planting.
The application of the taxus chinensis cutting cultivation method of any one of claims 1 to 5, wherein the taxus chinensis cutting cultivation method is applied to the technical field of plant cutting propagation.
Example two:
weighing the raw materials of the rooting solution in parts by weight according to the following formula: c12H10O222 parts of C10H9NO217 parts of C12H13NO228 parts of C12H10O311 parts of phenol compound 2.5 parts, 1150 parts of water and12H10O2、C10H9NO2、C12H13NO2、C12H10O3the phenol compound and water are fully mixed and stirred to obtain rooting liquid.
The matrix required by cutting cultivation comprises 16 parts of plant ash, 500 parts of crude river sand and 18 parts of farmyard manure.
Example three:
weighing the raw materials of the rooting solution in parts by weight according to the following formula: c12H10O230 portions of C10H9NO217 parts of C12H13NO217 portions of、C12H10O311 parts of phenol compound, 3 parts of water 1200 parts, and12H10O2、C10H9NO2、C12H13NO2、C12H10O3the phenol compound and water are fully mixed and stirred to obtain rooting liquid.
The matrix required by cutting cultivation comprises 20 parts of plant ash, 500 parts of crude river sand and 17 parts of farmyard manure.
Survival rate
Conventional technique 23%
Example one 83%
Example two 85%
EXAMPLE III 80%
In conclusion, the survival rate of the taxus chinensis is greatly improved compared with the traditional technology.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (6)

1. A cutting cultivation method of Chinese yew is characterized by comprising the following steps: firstly, selecting proper cutting seedlings in proper cutting time, preparing rooting liquid required by cutting, soaking the treated cutting seedlings in self-made rooting liquid for soaking treatment, preparing a matrix required by cutting cultivation, cultivating the treated cutting seedlings in the matrix, watering and fertilizing properly, and transplanting the survival Chinese yew seedlings cultivated in the matrix after one month.
2. The cutting cultivation method of taxus chinensis as claimed in claim 1, wherein the cutting cultivation time is selected from 3, 7 and 11 months per year, an annual branch is selected from healthy and strong taxus chinensis trees as a cutting shoot, the cutting shoot is cut into small sections of about 15 cm, and the top of each small section is provided with a plurality of leaves to prepare a cutting seedling.
3. The cutting cultivation method of taxus chinensis as claimed in claim 1, wherein the raw materials of the rooting solution in parts by weight are weighed according to the following formula: c12H10O220-30 parts of C10H9NO215-20 parts of C12H13NO28-9 parts of C12H10O310-12 parts of phenol compound, 2-3 parts of phenol compound, 1000 parts of water and 1200 parts of C12H10O2、C10H9NO2、C12H13NO2、C12H10O3The phenol compound and water are fully mixed and stirred to obtain rooting liquid.
4. The taxus chinensis cuttage cultivation method as claimed in claim 1, wherein the substrate required for cuttage cultivation comprises 15-20 parts of plant ash, 700 parts of crude river sand and 15-20 parts of farmyard manure, and the specific preparation method comprises the steps of separating crude river sand into A parts and B parts, wherein the A parts account for one fifth of the total amount of the crude river sand, the B parts account for four fifths of the total amount of the crude river sand, soaking the A parts of the crude river sand in rooting liquid, fully stirring and mixing the A parts of the crude river sand and the B parts of the crude river sand to obtain a substrate sand base, adding the plant ash and the farmyard manure into the substrate sand base, fully mixing and stirring to obtain a cultivation substrate, adding water to enable the substrate to be grabbed by hands, tightly holding the substrate, preventing water from dripping at finger joints, and preventing the substrate from scattering after the hands are loosened.
5. The cuttage cultivation method for taxus chinensis as claimed in claim 1, wherein the specific cuttage method for taxus chinensis is as follows:
checking the cutting seedlings to ensure that the cutting seedlings have activity and are healthy and strong, and soaking the qualified cutting seedlings in a rooting solution for 14-17 hours;
inserting the cutting seedlings soaked with the rooting solution into the prepared culture medium, wherein the insertion depth is four to five centimeters, placing the plants in a cool and ventilated place after the cutting, spraying a certain amount of water by using a spray can, and moving the plants to a light-scattering place for maintenance after one week;
and step three, cutting the yew seedlings cut in the step two for one month, digging the cut seedlings for inspection, and taking the healthy and alive yew seedlings for transplanting and planting.
6. The application of the taxus chinensis cutting cultivation method according to any one of claims 1 to 5, characterized in that the taxus chinensis cutting cultivation method is applied to the technical field of plant cutting propagation.
CN202110102942.0A 2021-01-26 2021-01-26 Taxus chinensis cutting cultivation method and application thereof Pending CN112753474A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202110102942.0A CN112753474A (en) 2021-01-26 2021-01-26 Taxus chinensis cutting cultivation method and application thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202110102942.0A CN112753474A (en) 2021-01-26 2021-01-26 Taxus chinensis cutting cultivation method and application thereof

Publications (1)

Publication Number Publication Date
CN112753474A true CN112753474A (en) 2021-05-07

Family

ID=75705663

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202110102942.0A Pending CN112753474A (en) 2021-01-26 2021-01-26 Taxus chinensis cutting cultivation method and application thereof

Country Status (1)

Country Link
CN (1) CN112753474A (en)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102960174A (en) * 2012-12-11 2013-03-13 雷学军 Method for breeding and cultivating Taxus chinensis var mairei through asexual cuttage
CN104396491A (en) * 2014-10-31 2015-03-11 江苏红豆杉生物科技股份有限公司 Taxus chinensis var. mairei cutting seedling-raising method
CN104885842A (en) * 2015-04-28 2015-09-09 江苏省中国科学院植物研究所 Method for cutting and breeding Taxus baccata
CN108522046A (en) * 2017-03-01 2018-09-14 长沙县俊武种养专业合作社 A kind of cuttage breeding method of Chinese yew nursery stock

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102960174A (en) * 2012-12-11 2013-03-13 雷学军 Method for breeding and cultivating Taxus chinensis var mairei through asexual cuttage
CN104396491A (en) * 2014-10-31 2015-03-11 江苏红豆杉生物科技股份有限公司 Taxus chinensis var. mairei cutting seedling-raising method
CN104885842A (en) * 2015-04-28 2015-09-09 江苏省中国科学院植物研究所 Method for cutting and breeding Taxus baccata
CN108522046A (en) * 2017-03-01 2018-09-14 长沙县俊武种养专业合作社 A kind of cuttage breeding method of Chinese yew nursery stock

Similar Documents

Publication Publication Date Title
CN1922987B (en) Multiple-ear rock lithocarpus glaber dwarfing rapid reproduction method
CN105613062B (en) A kind of rubber select tree tender tip grafting childrenization method for culturing seedlings
CN103125244A (en) Cutting propagation method of golden camellia plants
CN104380994A (en) Bletilla striata large-scale high-yield cultivation method
CN101960954A (en) Liquorice planting method
CN103563628A (en) Chinese torreya seedling raising method
CN104686274A (en) Efficient apocarya propagation method
CN109247238A (en) A kind of five leaflet maple tissue culture outside sprout-cultivating-bottle radication methods
CN100421542C (en) Grafting cultivation method for chrysanthemum morifolium
CN103109747B (en) Rapid pseudolarix propagation method based on stem node propagation
CN111685127B (en) Inducer for promoting vegetative propagation of plants and application thereof
CN105359972A (en) Wheat mature embryo callus induction culture and plant regeneration system construction method
CN106718763B (en) Sedum short-shoot cutting cultivation method
CN103039265B (en) Culture method for improving appearance quality of maca rootstocks
CN101564010B (en) Method for rapidly propagating tupelos
Pagani et al. BAP spray and plastic container responses on Asparagus officinalis L. crown growth
CN102893722A (en) Arborous Cercis canadensis seedling cultivation method
CN105875322A (en) Tea tree nutrient pot clonal rapid propagation method
CN103069980B (en) Cultivation method of dalmatian chrysan themum
CN105359905A (en) Caryaillinoensis high pressure seedling growing method
CN1234266C (en) Water moss implantation method
CN108112479A (en) A kind of stem section of papaya sprout Bud Differentiation vacantly plants leaf promoting root growth method
EP3089576B1 (en) Methods and compositions for production of watermelon fruit
CN112243791A (en) Method for cultivating clump sapium sebiferum
CN112753474A (en) Taxus chinensis cutting cultivation method and application thereof

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination