CN112715489A - Breeding method of ladybug with baitiao - Google Patents
Breeding method of ladybug with baitiao Download PDFInfo
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- CN112715489A CN112715489A CN202011603506.3A CN202011603506A CN112715489A CN 112715489 A CN112715489 A CN 112715489A CN 202011603506 A CN202011603506 A CN 202011603506A CN 112715489 A CN112715489 A CN 112715489A
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/05—Fruit crops, e.g. strawberries, tomatoes or cucumbers
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
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Abstract
The invention discloses a method for breeding ladybug with botrytis cinerea, which uses pumpkin powdery mildew as the living feed of the larva and seed worm of ladybug with botrytis cinerea, and then uses artificial feed as the main food of adult worm for breeding. The pumpkin powdery mildew is from pumpkin leaves infected with powdery mildew; the artificial feed is prepared from the following components in percentage by mass: 20% of glucose, 20% of fructose, 5% of soybean meal, 15% of silkworm chrysalis meal, 0.3% of feed-grade vitamin and 39.7% of sterile water, and the ingredients are cheap and easy to obtain and are rich in nutrition. By adopting the method to feed the ladybug, the adult acquisition rate is high, the egg laying period of the female beetles is long, and the egg laying amount is large. The obtained ladybug with baitiao bacteria is used for preventing and controlling plant powdery mildew, and has ideal effect, economy and environmental protection.
Description
Technical Field
The invention relates to the technical field of ladybug breeding, in particular to a method for breeding white streak fungus ladybug.
Background
Powdery mildew, belonging to the family of powdery mildew of the order erysiphe of the class sclerotinia of the subdivision ascomycete, is a pathogenic fungus which is obligately parasitic on the surface of plants and produces powdery mildew. When powdery mildew harms plants, the powdery mildew stretches into host cells by a sucker to absorb nutrient substances and continuously expands under epidermis, and the damaged parts of the plants gradually turn green and yellow, even wither and die of whole leaves. Generally speaking, powdery mildew has higher host specificity and can be divided into powdery mildew fungi which are specially infected with economic forest plants, such as roxburgh rose powdery mildew, apple powdery mildew, medlar powdery mildew and the like; the special infected crops comprise powdery mildew of cucumber, powdery mildew of wheat, powdery mildew of pepper and the like; the powdery mildew of Chinese rose, the powdery mildew of chrysanthemum, the powdery mildew of rose and the like which are specially infected with flowers. All the types of powdery mildew can occur year after year, which not only destroys the ecological balance, but also seriously damages the economic benefit of growers. As for the control technology of powdery mildew, chemical pesticides are mostly adopted for treatment at present, and because chemical agents are used for control scientifically for a long time, the environmental pollution is caused, the powdery mildew generates resistance to the chemical pesticides, and the pesticide effect is greatly reduced.
A Laggera albuginea (Halyzia hauseri Mader) belongs to the genus of yellow fungus ladybug belonging to the family of Coleoptera ladybug, and is a gram of powdery mildew of plants such as Rosa roxburghii Tratt and pumpkin. The ladybug is bred on a large scale, so that a large amount of natural enemy insects of powdery mildew are obtained, and then the natural enemy insects are utilized to treat plant powdery mildew, so that the use of pesticides can be effectively reduced, and the method is ideal in effect, economical and environment-friendly.
At present, the quantity and the scale of the brevibacillus bailii in natural environment are not large, and the research report about indoor breeding of the brevibacillus bailii is also few. To realize large-scale breeding of the ladybug, the food problem needs to be solved firstly, but the powdery mildew is a fungus which can not be cultured in vitro, so that the problem of how to simply, rapidly and massively breed the powdery mildew is difficult. The existing scheme is that the method for breeding the ladybug with the pathogenic leaves in a moisture-keeping mode is utilized, but the method has many limitations, a large amount of breeding time and labor are wasted, the movable area of the ladybug with the pathogenic leaves is small, and the phenomenon of self-disabled people is easily caused if the leaves are not replaced immediately. In conclusion, the conventional breeding method for breeding the brevibacterium sp has various defects, and the defects limit the large-scale breeding of the brevibacterium sp and are not beneficial to the industrialization of the brevibacterium sp.
Disclosure of Invention
The invention aims to provide a method for breeding ladybug with aschersonia aleyrodis, which solves the problem that the ladybug with aschersonia aleyrodis cannot be cultured simply, conveniently, rapidly and massively in the prior art.
The feeding method provided by the invention utilizes the characteristics of short growth cycle and low production environment requirement of the pumpkin to provide a food foundation for large-scale feeding of the ladybug with the aid of the pumpkin; the characteristics of low price, easy obtaining, easy preparation and easy storage of artificial feeding are utilized to provide nutrition for the large-scale feeding of the ladybug adults of the aschersonia aleyrodis; meanwhile, the invention also designs a collecting net for the eggs of the ladybug, which can simply and quickly collect the eggs of the ladybug. The technical scheme provided by the invention mainly comprises the following steps:
(1) hatching the eggs of the ladybug with the white streak: the collected eggs of ladybug were incubated in a petri dish (diameter 90mm) on which a piece of moist filter paper (diameter 70mm) was placed.
(2) Breeding of ladybug larvae by using the following steps: once the eggs of the ladybug are found to be hatched, all the eggs in the culture dish are immediately transferred to potted pumpkin leaves in the breeding box, the powdery mildew incidence area on the pumpkin leaves is checked every 24 hours, and the pumpkin potted plant with large incidence area is immediately replaced if 80% of the incidence area of the leaves is lower than 20%.
(3) And (3) breeding of the white streak fungus ladybug adults: the adult is mainly fed with artificial feed, and the adult is mainly fed with pumpkin powdery mildew (Podosphaera xanthothia).
(4) Collecting eggs of the ladybug with the white fungus: and (3) putting the 4 pairs of male and female seed worms and the egg collecting net into an egg laying box, feeding fresh leaves infecting pumpkin powdery mildew every 12 hours, and collecting worm eggs from the egg collecting net once every 24 hours.
Further, the method also comprises the step (5) of indoor potting planting of the pumpkins: the pumpkin is planted in an indoor pot culture mode, leaf fertilizer is sprayed once a week, when 8-10 leaves grow out, powdery mildew is inoculated on healthy pumpkin leaves by a leaf smearing method or a spore suspension method, the healthy pumpkin leaves are placed in a non-ventilated environment to be favorable for breeding the powdery mildew, and when 80% of the leaves are attacked, the pumpkin leaves can be used for feeding the ladybug larvae.
Further, the method also comprises the step (6) of preparing the artificial feed: the preparation method comprises the following steps of: 20% of glucose, 20% of fructose, 5% of soybean meal, 15% of silkworm chrysalis meal, 0.3% of feed-grade vitamin and 39.7% of sterile water.
The silkworm chrysalis powder and the soybean meal are dried, smashed and sieved by a 80-mesh screen, the soybean meal, the silkworm chrysalis powder and feed-grade vitamins are mixed and stirred uniformly, then the mixture is placed into a sterilization pot for sterilization for 15min, the mixture is taken out and cooled to room temperature, then sterile water and 0.2-0.4% of sorbic acid (preservative) are added, and the mixture is stirred uniformly.
The method induces the ladybug with the egg collection net to lay eggs on the egg collection net, and is used for collecting the eggs of the ladybug; in the larva breeding process, live pumpkins infected with powdery mildew are directly fed, the operation is simpler compared with moisturizing feeding by using infected leaves, the effect is better, and the survival rate of the ladybug is further improved.
Furthermore, in the step (1), the incubation environment temperature of the ladybug is 25-28 ℃, the humidity is 70-80%, and the illumination condition is 12L: 12D; the breeding environment temperature of the ladybug larvae and imagoes in the steps (2) and (3) is 25-28 ℃, the humidity is 70-80%, and the illumination condition is 14L: 10D; and (4) enabling the egg laying environment temperature of the ladybug with the brevibacterium sp to be 25-28 ℃, the humidity to be 70-80% and the illumination condition to be 14L: 10D.
Furthermore, the method for manufacturing the egg collecting net in the step (4) comprises the following steps: folding a white gauze (PP material) with the size of A4 paper and the aperture of about 1.2-1.4 mm from the middle to ensure that the included angle after the gauze is normally unfolded is preferably 30-90 degrees, soaking the gauze for 30s by using 10% of honey water, and naturally airing the gauze for use.
Furthermore, the culture dish, the breeding box, the spawning box and other high-temperature resistant appliances need to be sterilized by a steam sterilizer before use, and the sterilization conditions are as follows: the temperature is 120 ℃, the time is 15-30 min, and instruments which cannot be sterilized at high temperature need to be sterilized by using 75% alcohol for more than 30 s.
Furthermore, in the step (5), the indoor potted planting of the pumpkin is carried out at the environment temperature of 25-28 ℃, and the pot is cylindrical, the diameter of the pot is about 120mm, and the height of the pot is about 130 mm. Watering once a day after planting, and applying 0.3-0.5% compound fertilizer aqueous solution and 2% pumpkin leaf fertilizer aqueous solution 1 time each week after sprouting; the diseased pumpkin and the healthy pumpkin must be planted separately, so that powdery mildew is prevented from being infected in advance, and normal growth is prevented from being influenced.
Furthermore, the breeding box in the step (2) is rectangular, and made of acryl materials, and the length, the width and the height of the breeding box are 40cm, 30cm and 50cm respectively; the front, back, left and right sides are provided with a vent of 10cm x 10cm, and the vent is sealed by using a gauze (PP material) with a fixed aperture of about 0.5mm and double-sided adhesive tape to prevent larvae from climbing out. The top of the feeding box is detachably designed and has better sealing property; adults successfully eclosion in the rearing boxes were observed and collected every day.
Furthermore, the oviposition box in the step (4) is rectangular, PP material is used, and the length, the width and the height of the oviposition box are respectively 30cm, 20cm and 15 cm; the top of the bag is detachably designed, and is provided with a 2cm multiplied by 2cm air hole, and a gauze (PP material) with the aperture of about 1mm is fixed by double-sided adhesive tape to seal the bag.
Compared with the prior art, the invention has the following remarkable effects:
the method can effectively avoid the defects of complex operation, time and labor consumption, low survival rate and the like in the traditional moisturizing feeding of the leaves infected with powdery mildew. The invention adopts the potted pumpkin living body breeding powdery mildew as the main feed of the ladybug larvae and the seed worms, has mature planting technology, fast growth, large leaves, high morbidity, easy inoculation, low cost, no influence of seasonal factors and capability of stably feeding sufficient food for the ladybug all the year round; the adult of the ladybug is the period of eating the largest amount of powdery mildew, and for the adult without the seed worm, the consumption of the powdery mildew can be reduced to a great extent by using artificial feed instead of the adult, so that the feeding complexity is further reduced; the egg collecting net designed by the method can effectively attract the ladybug to lay eggs on the egg collecting net, reduces the egg collecting difficulty and the artificial damage rate, and can promote the female to lay eggs and avoid the situation that the female eats the eggs by itself by collecting the eggs regularly. In practice, the method has the advantages of simple and convenient operation, ideal development rate of the ladybug, low mortality, high adult yield, long egg laying period of female worms, large egg laying amount and the like.
The specific implementation mode is as follows:
the technical solutions in the embodiments of the present invention are clearly and completely described below. The technical features mentioned in the embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other. The ingredients or materials involved in the following processes, unless otherwise specified, are commercially available. The related experimental methods are conventional methods in the technical field if not specifically stated. The numerical values or numerical proportions, if not indicated, are mass values or mass proportions.
Example 1:
the pumpkin powdery mildew is used as the living feed of the larva and the seed worm of the ladybug, the artificial feed is used as the main food of the adult, and the feeding method comprises the following steps:
(1) preparation before raising
All culture dishes, raising boxes, spawning boxes and other high temperature resistant appliances need to be sterilized by a steam sterilizer before use, and the sterilization conditions are as follows: the temperature is 120 ℃, the time is 15-30 min, and the appliances which cannot be sterilized at high temperature need to be sterilized by using 75% alcohol for more than 30s before use.
Soaking several pumpkin seeds for 4h, and preparing 10% bee water.
An egg collection net was made as designed. The egg collecting net is made of white gauze (PP) with the size of A4 paper, the aperture is about 1.2-1.4 mm, the egg collecting net is folded from the middle, the included angle after the egg collecting net is normally unfolded is preferably 30-90 degrees, the egg collecting net is soaked in 10% of honey water for 30s, and the egg collecting net is obtained after natural drying.
(2) Indoor potted planting of pumpkin
Preparing a plurality of cylindrical basins with the diameter of about 120mm and the height of about 130mm, firstly putting two thirds of soil, slightly compacting, then putting the soaked seeds, and then filling the soil without compacting. And (3) watering for 1 month at the ambient temperature of 25-28 ℃, watering for 1 time every day, and dressing 0.3-0.5% compound fertilizer aqueous solution and 2% pumpkin leaf surface fertilizer aqueous solution for 1 time every 7 days after sprouting. When 8-10 leaves grow out, inoculating powdery mildew by using a leaf smearing method or a spore suspension method, then placing the inoculated powdery mildew in a non-ventilated environment for culturing for 7d, observing the disease occurrence condition, and when about 80% of the disease occurrence area of the leaves exceeds 60%, feeding the ladybug with the fungus; the pumpkin potted plant infected with powdery mildew and the normal pumpkin potted plant are planted in different places to prevent the pumpkin potted plant from being infected with powdery mildew in advance and influencing normal growth.
(3) Making artificial feed
The preparation method comprises the following steps of: 20% of glucose, 20% of fructose, 5% of soybean meal, 15% of silkworm chrysalis meal, 0.3% of feed-grade vitamin and 39.7% of sterile water.
The silkworm chrysalis meal and the soybean meal are dried, smashed and sieved by a 80-mesh screen, the soybean meal, the silkworm chrysalis meal and feed-grade vitamins are mixed and stirred uniformly, then the mixture is put into a sterilization pot for sterilization for 15min, the mixture is taken out and cooled to room temperature, then sterile water and 0.2-0.4% of sorbic acid (preservative) are added, and the mixture is stirred uniformly.
(4) Hatching of white streak fungus ladybug eggs
Wetting a piece of filter paper with the diameter of 70mm by using water, then placing the filter paper into a culture dish with the diameter of 90mm, transferring all eggs on an egg collecting net onto the filter paper in the culture dish, covering the culture dish, and placing the culture dish into an environment with the temperature of 25-28 ℃, the humidity of 70-80% and the illumination condition of 12L:12D, wherein the egg period of the ladybug is about 3D under the condition.
(5) Breeding of ladybug larvae
Once the eggs of the ladybug are found to be hatched, all the eggs in the culture dish are immediately transferred to potted pumpkin leaves in the breeding box, the powdery mildew incidence area on the pumpkin leaves is checked every 24 hours, and if 80% of the incidence area of the leaves is lower than 20%, the pumpkin potted plants with large incidence areas are immediately replaced; the breeding box needs to be placed in an environment with the temperature of 25-28 ℃, the humidity of 70-80% and the illumination condition of 14L: 10D; the larval stage of the ladybug is about 14d under the environment; the feeding box is rectangular, made of acryl material, and has a length, a width and a height of 40cm, 30cm and 50cm respectively; the front, back, left and right sides are provided with a vent of 10cm x 10cm, and the vent is sealed by using a gauze (PP material) with a fixed aperture of about 0.5mm and double-sided adhesive tape to prevent larvae from climbing out. The top of the feeding box is detachably designed and has better sealing property; adults successfully eclosion in the rearing boxes were observed and collected every day.
(6) Breeding of white-striped fungus ladybug adults
The adult is mainly fed with artificial feed, and the adult as the seed insect is mainly fed with pumpkin powdery mildew; the temperature of the environment for raising the imagoes and the seed insects is 25-28 ℃, the humidity is 70-80%, and the illumination condition is 14L: 10D;
(7) collection of eggs of ladybug
Putting 4 pairs of wild harmonia axyridis male and female harmonia axyridis and an egg collecting net into a spawning box, and placing the spawning box in an environment with the temperature of 25-28 ℃, the humidity of 70-80% and the illumination condition of 14L: 10D; feeding fresh leaves of powdery mildew of pumpkin every 12h, and collecting worm eggs from the egg collecting net every 24 h. The oviposition box is cuboid, adopts PP material, and has length, width and height of 30cm, 20cm and 15cm respectively; the top of the bag is detachably designed, and is provided with a 2cm multiplied by 2cm air hole, and a gauze (PP material) with the aperture of about 1mm is fixed by double-sided adhesive tape to seal the bag.
Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes in the embodiments and/or modifications of the invention can be made, and equivalents and modifications of some features of the invention can be made without departing from the spirit and scope of the invention.
Claims (10)
1. A method for breeding ladybug of white streak fungus is characterized in that: the method uses pumpkin powdery mildew as the living feed of the larva and the seed worm of the ladybug, and then uses the artificial feed as the main food of the adult for feeding.
2. The breeding method of ladybug according to claim 1, characterized by comprising the steps of: 1) hatching the eggs of the ladybug with the white streak: putting the collected eggs of the ladybug into a culture dish padded with a piece of wet filter paper for incubation; 2) breeding of ladybug larvae by using the following steps: after hatching the eggs of the ladybug, transferring all the eggs in the culture dish to potted pumpkin leaves in the breeding box, checking powdery mildew incidence areas on the pumpkin leaves every 24 hours, and immediately replacing the potted pumpkin with large incidence areas if 80% of the incidence areas of the leaves are lower than 20%; 3) and (3) breeding of the white streak fungus ladybug adults: the adult is mainly fed with artificial feed, and the adult as the seed insect is mainly fed with pumpkin powdery mildew; 4) collecting eggs of the ladybug with the white fungus: and (3) putting the 4 pairs of male and female seed worms and the egg collecting net into an egg laying box, feeding fresh pumpkin powdery mildew leaves every 12h, and collecting worm eggs from the egg collecting net once every 24 h.
3. The breeding method of ladybug according to claim 1 or 2, characterized by further comprising the steps of: 5) indoor potted planting of pumpkins: the pumpkin is planted in an indoor pot culture mode, compound fertilizer and leaf fertilizer are applied once every week, when 8-10 leaves grow out, powdery mildew is inoculated to the healthy pumpkin leaves by using a leaf smearing method or a spore suspension method, then the healthy pumpkin leaves are placed in a non-ventilated environment for breeding, and when 80% of the leaves reach 60%, the pumpkin leaves can be placed in a breeding box for feeding.
4. The breeding method of ladybug according to claim 1 or 2, characterized by further comprising the steps of: 6) preparing artificial feed: the preparation method comprises the following steps of: 20% of glucose, 20% of fructose, 5% of soybean meal, 15% of silkworm chrysalis meal, 0.3% of feed-grade vitamin and 39.7% of sterile water.
5. The breeding method of ladybug with sakayama according to claim 2, characterized in that: the manufacturing method of the egg collecting net in the step 4) comprises the following steps: folding a PP white gauze with the size of A4 paper and the aperture of 1.2-1.4 mm from the middle to ensure that the included angle after the gauze is normally unfolded is 30-90 degrees, soaking the gauze in 10 percent of honey water for 30s, and naturally drying the gauze.
6. The breeding method of ladybug with sakayama according to claim 2, characterized in that: in the step 1), the incubation environment temperature of the ladybug with the brevibacterium sp is 25-28 ℃, the humidity is 70-80%, and the illumination condition is 12L: 12D; in the step 2) and the step 3), the breeding environment temperature of the ladybug larvae and the imagoes is 25-28 ℃, the humidity is 70-80%, and the illumination condition is 14L: 10D; in the step 4), the egg-laying environment temperature of the ladybug is 25-28 ℃, the humidity is 70-80%, and the illumination condition is 14L: 10D.
7. The breeding method of ladybug according to claim 1 or 2, characterized in that: the culture dish, the breeding box, the spawning box and other high-temperature resistant appliances adopted by the method are sterilized by a steam sterilizer before use, and the sterilization conditions are as follows: the temperature is 120 ℃, the time is 15-30 min, and instruments which cannot be sterilized at high temperature are sterilized by using 75% alcohol for more than 30 s.
8. The breeding method of ladybug with sakayama according to claim 3, characterized in that: step 5), the indoor potted planting environment temperature of the pumpkin is 25-28 ℃; watering once a day after planting, and applying 0.3-0.5% compound fertilizer aqueous solution and 2% pumpkin leaf fertilizer aqueous solution 1 time each week after sprouting; diseased pumpkins and healthy pumpkins are planted separately.
9. The breeding method of ladybug with sakayama according to claim 3, characterized in that: the shape of the feeding box in the step 2) is a cuboid, the feeding box is made of acryl materials, and the length, the width and the height of the feeding box are respectively 40cm, 30cm and 50 cm; the front, back, left and right sides are provided with a vent hole of 10cm multiplied by 10cm, and gauze with the aperture of about 0.5mm is fixed by double-sided adhesive tape to seal the vent hole; the top of the feeding box is detachably designed and has sealing performance; adults successfully eclosion in the rearing boxes were observed and collected every day.
10. The breeding method of ladybug with sakayama according to claim 3, characterized in that: the shape of the oviposition box in the step 4) is a cuboid, and the length, width and height of the oviposition box are respectively 30cm, 20cm and 15 cm; the top of the bag is detachably designed, and is provided with a 2cm multiplied by 2cm air hole, and the bag is sealed by a gauze fixed by double-sided adhesive tape.
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