CN112494414B - Traditional Chinese medicine compound with whitening and anti-aging effects and preparation method thereof - Google Patents
Traditional Chinese medicine compound with whitening and anti-aging effects and preparation method thereof Download PDFInfo
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- 238000002360 preparation method Methods 0.000 title claims abstract description 18
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
- A61K8/981—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of mammals or bird
- A61K8/986—Milk; Derivatives thereof, e.g. butter
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
- A61K8/981—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of mammals or bird
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
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Abstract
The invention discloses a traditional Chinese medicine compound with whitening and anti-aging effects, which comprises the following components: cornu Cervi Pantotrichum, radix asparagi, glycyrrhrizae radix, rhizoma Bletillae, radix Ampelopsis, atractylodis rhizoma, rhizoma Ligustici Chuanxiong, semen Armeniacae amarum, and Lac bovis Seu Bubali. The invention also discloses a preparation method of the traditional Chinese medicine compound, which comprises the steps of material selection, cleaning, drying, grinding, primary water bath enzymolysis, secondary water bath enzymolysis and preparation. According to the invention, cornu cervi pantotrichum is adopted to replace cornu cervi degelatinatum in the traditional antler paste, bletilla striata is adopted to replace radix angelicae, ligusticum wallichii is adopted to replace asarum, and liquorice is added to enhance the whitening and anti-aging effects and simultaneously harmonize the other medicines. Improves the whitening and anti-aging effects on the basis of eliminating the toxic and side effects. The mode of firstly carrying out enzymolysis on the compound raw materials by using the biological enzyme to break cell walls and then extracting is more advanced and reasonable, the extraction efficiency of each raw material is improved, and the dissolution of active substances is more facilitated.
Description
Technical Field
The invention relates to the field of cosmetics, in particular to a traditional Chinese medicine compound with whitening and anti-aging effects and a preparation method thereof.
Background
Antler paste, originated from the Taiping Shenghui Fang, is a facial cream prepared by taking antler powder as a main drug. Cornu Cervi Degelatinatum is the residue left after decocting cornu Cervi, and has effects of warming, entering liver and kidney channels, invigorating essence and blood, nourishing blood, promoting facial blood circulation, and enhancing skin nutrition, thereby playing the role of retaining youthful looks and caring skin. The cornu Cervi paste is prepared from cornu Cervi Degelatinatum, lac bovis seu Bubali, radix Angelicae Dahuricae, rhizoma Ligustici Chuanxiong, herba asari, radix Angelicae Dahuricae, rhizoma Typhonii, atractylodis rhizoma, semen Armeniacae amarum, radix asparagi and butyrum. Contains a large amount of animal protein, fat, various vitamins and minerals, and is necessary for human body to nourish skin. Bighead atractylodes rhizome and bletilla striata also contain glucose, protein, fat and vitamins and have the function of nourishing the skin. The ampelopsis japonica has different degrees of inhibition effects on dermatophytes; the hemlock parsley, the angelica dahurica, the almond and the like have the effect of resisting tyrosinase, can reduce the generation of age pigment melanin, can tender the face and resist wrinkles, and can whiten and fine the skin, thereby being a good cosmetic product commonly used in traditional Chinese medicines. The medicines are combined and decocted to form the ointment for coating the face, so that the nutrition of the face and the skin can be improved, the wrinkles can be eliminated, and the youthful appearance can be restored. "Chinese medicine beauty paper selection
Although traditional antler paste has such good cosmetic efficacy, the formulation has some disadvantages due to its historical limitations. For example, the cornu cervi degelatinatum of the formula has very limited efficacy as bone residues after the deer-horn glue is decocted; the formula adopts a large amount of radix angelicae, asarum and rhizoma typhonii, the radix angelicae has photosensitivity, and the asarum and the rhizoma typhonii have large irritation to skin, and the asarum and the rhizoma typhonii are forbidden in modern cosmetic raw material list tests in China.
Disclosure of Invention
In order to solve the defects, the invention aims to provide a traditional Chinese medicine compound with whitening and anti-aging effects, and the whitening and anti-aging effects are improved on the basis of eliminating toxic and side effects of the traditional Chinese medicine compound.
The invention also aims to provide a preparation method of the traditional Chinese medicine compound with the whitening and anti-aging effects, the preparation method is simple to operate, and the biological enzyme is reasonably used for carrying out the mode of firstly carrying out enzymolysis on cell walls and then extracting, so that the dissolution of active substances is facilitated.
In order to achieve the purpose, the invention provides a traditional Chinese medicine compound with whitening and anti-aging effects, which comprises the following components in parts by weight: 3-6 parts of pilose antler, 2-4 parts of asparagus, 2-4 parts of liquorice, 1-2 parts of bletilla striata, 1-2 parts of ampelopsis japonica, 1-2 parts of bighead atractylodes rhizome, 1-2 parts of ligusticum wallichii, 1-2 parts of almond and 6-12 parts of cow milk.
The invention also provides a preparation method of the traditional Chinese medicine compound with whitening and anti-aging effects, which comprises the following steps:
(1) Selecting materials: selecting high-quality medicinal materials as raw materials;
(2) Cleaning: cleaning cornu Cervi Pantotrichum, radix asparagi, glycyrrhrizae radix, rhizoma Bletillae, radix Ampelopsis, atractylodis rhizoma, rhizoma Ligustici Chuanxiong, and semen Armeniacae amarum;
(3) And (3) drying: drying the cleaned medicinal materials in the step (2) at a low temperature to obtain a dry mixture;
(4) Grinding: grinding the dried mixture to a fine powder of 20-100 mesh;
(5) Primary water bath enzymolysis: weighing 200-400 parts of the fine powder, adding 100-200 parts of cow milk, 0.2-0.4 part of cellulase and 1000-2000 parts of pure water, and performing enzymolysis in water bath at 40-50 ℃ for 1-2h;
(6) Secondary water bath enzymolysis: adding 0.1-0.2 part of protease, and performing enzymolysis in water bath at 40-50 deg.C for 1-2h;
(7) Preparation: adding 1000-2000 parts of 95% ethanol, refluxing at 60-70 deg.C for 4-6 hr, filtering, concentrating the filtrate until no ethanol exists, adding 1,3-butanediol, dissolving completely, and filtering to obtain the Chinese medicinal composition.
Further, in the step (7), the filtered filtrate is heated to 60-70 ℃ for concentration.
Further, in the step (7), 1,3-butanediol is added in an amount equal to the mass of the concentrated solution.
According to the invention, cornu cervi pantotrichum is adopted to replace cornu cervi degelatinatum in the traditional antler paste, and angelica dahurica is replaced by bletilla with good whitening effect; herba asari with warming and invigorating yang effects is replaced by rhizoma Ligustici Chuanxiong, and Glycyrrhrizae radix is added to enhance its whitening and antiaging effects and simultaneously harmonize the above drugs; the compound raw materials are subjected to enzymolysis firstly to break cell walls and then to extraction by using the biological enzyme, so that the extraction efficiency of each raw material is improved; the cow milk in the compound is subjected to enzymolysis by using specific protease, so that macromolecular proteins are decomposed into micromolecular amino acids and polypeptides, and the absorption and the permeation of skin are facilitated.
The invention has the beneficial effects that: raw materials with toxic and side effects and irritation to the skin are reasonably replaced, and protein components which are difficult to absorb by the skin are converted into amino acids and polypeptides which are easier to absorb, so that the whitening and anti-aging effects of the skin-care product are improved on the basis of eliminating the toxic and side effects. Compared with the traditional rough boiling mode, the invention reasonably uses the biological enzyme to carry out the mode of firstly carrying out enzymolysis on cell walls and then extracting, and the preparation method is more advanced and reasonable and is more beneficial to dissolving out active substances.
Drawings
FIG. 1 is a graph of the results of a conventional antler paste removal DPPH test.
FIG. 2 is a graph of the DPPH clearance test results of the herbal composition of example 1.
FIG. 3 is a graph showing the results of inhibition of tyrosinase activity by conventional antler paste.
FIG. 4 is a graph showing the results of the inhibition of tyrosinase activity by the herbal composition of example 2.
Detailed Description
The present invention will now be described in detail with reference to the drawings and specific embodiments, wherein the exemplary embodiments and descriptions of the present invention are provided to explain the present invention without limiting the invention thereto.
Example 1:
a traditional Chinese medicine compound with whitening and anti-aging effects comprises the following components in parts by weight: 3 parts of pilose antler, 2 parts of cochinchnese asparagus root, 2 parts of liquorice, 1 part of bletilla striata, 1 part of ampelopsis japonica, 1 part of bighead atractylodes rhizome, 1 part of szechuan lovage rhizome, 1 part of almond and 6 parts of cow milk.
A preparation method of a traditional Chinese medicine compound with whitening and anti-aging effects comprises the following steps:
(1) Selecting materials: selecting high-quality medicinal materials as raw materials;
(2) Cleaning: cleaning cornu Cervi Pantotrichum, radix asparagi, glycyrrhrizae radix, rhizoma Bletillae, radix Ampelopsis, atractylodis rhizoma, rhizoma Ligustici Chuanxiong, and semen Armeniacae amarum;
(3) And (3) drying: drying the cleaned medicinal materials in the step (2) at a low temperature to obtain a dry mixture;
(4) Grinding: grinding the dried mixture to a fine powder of 20 mesh;
(5) Primary water bath enzymolysis: weighing 200 parts of the fine powder, adding 100 parts of cow milk, 0.2 part of cellulase and 1000 parts of pure water, and carrying out water bath enzymolysis for 1h at 40 ℃;
(6) Secondary water bath enzymolysis: adding 0.1 part of protease, and carrying out enzymolysis for 1h in water bath at 40 ℃;
(7) Preparation: adding 1000 parts of 95% ethanol, condensing and refluxing for 4h at 60 ℃, filtering, heating the filtrate to 60 ℃, concentrating until no alcohol exists, adding 1,3-butanediol with the same mass as the concentrated solution, completely dissolving, and filtering to obtain the traditional Chinese medicine compound.
Example 2:
a traditional Chinese medicine compound with whitening and anti-aging effects comprises the following components in parts by weight: 6 parts of pilose antler, 4 parts of cochinchnese asparagus root, 4 parts of liquorice, 2 parts of bletilla striata, 2 parts of ampelopsis japonica, 2 parts of largehead atractylodes rhizome, 2 parts of szechuan lovage rhizome, 2 parts of almond and 12 parts of cow milk.
A preparation method of a traditional Chinese medicine compound with whitening and anti-aging effects comprises the following steps:
(1) Selecting materials: selecting high-quality medicinal materials as raw materials;
(2) Cleaning: cleaning cornu Cervi Pantotrichum, radix asparagi, glycyrrhrizae radix, rhizoma Bletillae, radix Ampelopsis, atractylodis rhizoma, rhizoma Ligustici Chuanxiong, and semen Armeniacae amarum;
(3) And (3) drying: drying the cleaned medicinal materials in the step (2) at a low temperature to obtain a dry mixture;
(4) Grinding: grinding the dried mixture to a fine powder of 100 mesh;
(5) Primary water bath enzymolysis: weighing 400 parts of the fine powder, adding 200 parts of cow milk, 0.4 part of cellulase and 2000 parts of pure water, and carrying out enzymolysis in a water bath at 50 ℃ for 2 hours;
(6) Secondary water bath enzymolysis: adding 0.2 part of protease, and carrying out enzymolysis for 2h in water bath at 50 ℃;
(7) Preparation: adding 2000 parts of 95% ethanol, condensing and refluxing at 70 ℃ for 6 hours, filtering, heating the filtrate to 70 ℃, concentrating until no alcohol exists, adding 1,3-butanediol with the same mass as the concentrated solution, completely dissolving, and filtering to obtain the traditional Chinese medicine compound.
The efficacy test of the herbal composition prepared in this example 1 is performed as follows.
1. Comparative test for DPPH radical scavenging of traditional antler paste and the herbal composition prepared in example 1:
1. the purpose is as follows: the DPPH radical scavenging effect of the traditional antler paste and the traditional Chinese medicine composition of example 1 was measured.
2. Sample preparation: traditional antler paste, the traditional Chinese medicine composition of example 1.
3. Reagent:
(1) 3.943mgDPPH was made up to 50mL with 95% ethanol
(2) About 10mg of Trolox is added with 95% ethanol to be 100mL.
4. Experimental procedure
(1) UV zero calibration, 95% ethanol as blank
(2) Preliminary experiments (DPPH concentration selection)
Shaking 500 μ L DPPH +1000 μ L95% ethanol for 10s, standing for 10min, and measuring absorbance (absorbance at 520nm of 0.45-0.55, can be used as DPPH solution for the following experiment)
(3) Determination of Trolox
| Sample | Trolox（μL） | 95% ethanol (μ L) | DPPH（μL） |
| |
100 | 900 | 500 |
| |
100 | 900+500 | 0 |
| |
0 | 100+900 | 500 |
| |
0 | 100+900+500 | 0 |
Adding DPPH or 95% ethanol, shaking for 10s, standing for 10min, and measuring absorbance
(4) Experiment of
S:
SB: 
C: 
CB: 
Adding DPPH or 95% ethanol, shaking for 10s, and standing for 10min
(5) Measuring absorbance at 520nm, making table, and plotting to obtain IC50 value
Calculating the formula: inhibition (%) = [1- (S-SB)/(C-CB) ] + 100.
5. Results
Conventional antler paste cleared DPPH results table:
S：Sample SB：Sample Blank C：Control CB：Control Blank
conventional antler paste removal DPPH test results: as shown in fig. 1.
The results of the removal of DPPH for the herbal composition of example 1 are shown in the table:
the graph of the DPPH test results for the clearance of the herbal combination of example 1: as shown in fig. 2.
And (4) conclusion: the traditional antler paste and the traditional Chinese medicine compound in the embodiment 1 have good effect of eliminating DPPH, and the DPPH eliminating, namely the anti-oxidation and anti-aging effect of the traditional Chinese medicine compound in the embodiment 1 is better than that of the traditional antler paste.
2. Test comparison of tyrosinase inhibition by traditional antler paste and the traditional Chinese medicine composition of example 2:
1. the purpose is as follows: the inhibitory effect of the antler paste and the Chinese medicinal composition of example 2 on tyrosinase activity was measured.
2. Materials: traditional antler paste, the traditional Chinese medicine composition of example 2.
3. Sample preparation:
buffer solution: k2HPO4 (8.71 g/500 mL) + KH2PO4 (6.805 g/500 mL) → pH =6.80 (± 0.02)
Substrate: about 0.3mg/mL of L-tyrosine
A spectrophotometer: uv, 480nm.
4. The method comprises the following steps:
(1) Enzyme concentration selection:
taking a small amount of enzyme, dissolving the enzyme by using a buffer solution, wherein the color is brown; adding 500. Mu.L of (L-tyrosine (0.3 mg/mL)), 500. Mu.L of buffer solution, 450. Mu.L of pure water and 50. Mu.L of enzyme into a 1.5mL centrifuge tube; before adding enzyme, water bath is carried out for 20min at 37 ℃; after the addition of the enzyme, the absorbance was measured at a wavelength of 480nm in a spectrophotometer at 37 ℃ in a water bath (enzyme addition-test) for 10 min. The enzyme concentration was adjusted to give an Absorbance (ABS) of 0.45-0.55. The enzyme solution at this concentration was used as an experimental enzyme solution.
(2) Experimental addition
(3) Absorbance at 480nm was measured, and a table and a graph were drawn to obtain IC50.
Calculating the formula: inhibition rate (%) = [1- (S-SB)/(C-CB) ] = 100
Note that: before adding enzyme, water bath is carried out at 37 ℃ for 20min; after adding enzyme, water bath at 37 ℃ (adding enzyme-testing time) for 10min for light splitting
The absorbance was measured at a wavelength of 480nm. Without enzyme, in 37 deg.C water bath for 20min.
5. Results
The results of the inhibition of tyrosinase activity by traditional antler paste are shown in the table:
| amount used (μ L) | Concentration (μ L/mL) | Inhibition ratio% |
| 90 | 60 | 59.89% |
| 60 | 40 | 42.13% |
| 30 | 20 | 26.85% |
Results of tyrosinase activity inhibition by traditional antler paste: as shown in fig. 3.
The results of the inhibition of tyrosinase activity by the herbal compound of this example 2 are shown in the table:
S：Sample SB：Sample Blank C：Control CB：Control Blank
| amount of use (mu L) | Concentration (μ L/mL) | |
| 10 | 7 | 18.41% |
| 20 | 13 | 39.57% |
| 30 | 20 | 62.59% |
The result of the tyrosinase activity inhibition by the Chinese herbal compound of the embodiment 2 is shown in the figure: as shown in fig. 4.
And (4) conclusion: the traditional antler paste and the traditional Chinese medicine compound in the embodiment 2 have good tyrosinase inhibition effect, and the traditional Chinese medicine compound in the embodiment 2 has better inhibition effect on tyrosinase than the traditional antler paste, namely, the traditional Chinese medicine compound in the embodiment 2 has better whitening effect than the traditional antler paste.
Claims (3)
1. The traditional Chinese medicine compound with the effects of whitening and resisting aging is characterized by comprising the following components in parts by weight: 3-6 parts of pilose antler, 2-4 parts of asparagus, 2-4 parts of liquorice, 1-2 parts of bletilla striata, 1-2 parts of ampelopsis japonica, 1-2 parts of bighead atractylodes rhizome, 1-2 parts of ligusticum wallichii, 1-2 parts of almond and 6-12 parts of cow milk,
the preparation method of the traditional Chinese medicine compound with whitening and anti-aging effects comprises the following steps:
(1) Selecting materials: selecting high-quality medicinal materials as raw materials;
(2) Cleaning: cleaning cornu Cervi Pantotrichum, radix asparagi, glycyrrhrizae radix, rhizoma Bletillae, radix Ampelopsis, atractylodis rhizoma, rhizoma Ligustici Chuanxiong, and semen Armeniacae amarum;
(3) And (3) drying: drying the cleaned medicinal materials in the step (2) at a low temperature to obtain a dry mixture;
(4) Grinding: grinding the dried mixture to a fine powder of 20-100 mesh;
(5) Primary water bath enzymolysis: weighing 200-400 parts of the fine powder, adding 100-200 parts of cow milk, 0.2-0.4 part of cellulase and 1000-2000 parts of pure water, and performing enzymolysis in water bath at 40-50 ℃ for 1-2h;
(6) Secondary water bath enzymolysis: adding 0.1-0.2 part of protease, and performing enzymolysis in water bath at 40-50 deg.C for 1-2h;
(7) Preparation: adding 1000-2000 parts of 95% ethanol, condensing and refluxing at 60-70 deg.C for 4-6h, filtering, concentrating the filtrate until no alcohol is present, adding 1,3-butanediol, dissolving completely, and filtering to obtain the Chinese medicinal composition.
2. The traditional Chinese medicine compound with whitening and anti-aging effects as claimed in claim 1, is characterized in that: in the step (7), the filtered filtrate is heated to 60-70 ℃ for concentration.
3. The traditional Chinese medicine compound with whitening and anti-aging effects as claimed in claim 1, is characterized in that: in the step (7), the added 1,3-butanediol has the same mass as the concentrated solution.
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| 鹿角膏对老龄小鼠皮肤超氧化物歧化酶活力影响的实验研究;李凌霞等;《中国中医药科技》;20100731;第17卷(第4期);第347页 * |
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