CN106265362A - A kind of whitening sun protection skin care compositions and preparation method and application - Google Patents
A kind of whitening sun protection skin care compositions and preparation method and application Download PDFInfo
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- CN106265362A CN106265362A CN201610862056.7A CN201610862056A CN106265362A CN 106265362 A CN106265362 A CN 106265362A CN 201610862056 A CN201610862056 A CN 201610862056A CN 106265362 A CN106265362 A CN 106265362A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/533—Leonurus (motherwort)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/75—Rutaceae (Rue family)
- A61K36/754—Evodia
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/898—Orchidaceae (Orchid family)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/04—Topical preparations for affording protection against sunlight or other radiation; Topical sun tanning preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/10—General cosmetic use
Abstract
The invention belongs to natural product extraction and separation technology field, be specifically related to a kind of whitening sun protection skin care compositions and preparation method and application.Described whitening sun protection skin care compositions is passed through CO by raw material components Fructus Evodiae, Radix Hamamelidis Mollis, Herba Leonuri and the Pseudobulbus Bletillae (Rhizoma Bletillae)2The steps such as supercritical fluid extraction, supertension assisted extraction and microwave radiation exaraction prepare.The present invention passes through reasonable compatibility, different plant effective active compositions is made to produce synergistic function, excite the property of medicine of effective active matter greatly, the whitening sun protection skin care compositions prepared has sun-proof, whitening, speckle dispelling, antiallergic, the effect such as antibacterial, can be widely applied to skin care field.
Description
Technical field
The invention belongs to natural product extraction and separation technology field, be specifically related to a kind of whitening sun protection skin care compositions and
Its preparation method and application.
Background technology
Along with improving constantly of modern life level, increasing consumer starts to pay attention to i.e. being harmful to human health,
There are again the natural skin care cosmetics of beauty and health care functions.Utilize Chinese herbal medicine extract as the interpolation of beauty and skin care cosmetics
Agent, has efficacy stability lasting, not only produces effect but also do not have the advantage of toxic and side effects, deeply favored by people.At present, Chinese herbal medicine
Contained various active skull cap components, are widely used in modern beauty skin protection cosmetics, and play various effect.
Chinese herbal medicine extract can be explored from 3 directions by compounding in beauty and skin care cosmetic field: (1) with
The additive compound of non-plant origin, to reaching intended effect；(2) compound based on vegetarian purified thing, so
Formula, be chemically clear and definite on composition；(3) continue the tradition of Chinese herbal medicine, based on compound Chinese herb medical material, carry out multiple
Side extracts.Although Chinese herbal medicine extract can be further strengthened for beauty and skin care cosmetic formulations, but have to time compounding
Consider its limitation used, including its purity, safety, effectiveness and dosage form etc..
Additionally, the extraction separation of Chinese herbal medicine effective ingredients is the key link of beauty and skin care Cosmetics Production, its work
Skill feature, the selection of technological process and equipment configure and are all directly connected to be extracted the quality and quantity of effective ingredient, thus shadow
Ring stability and the effectiveness etc. arriving product quality.And, the development of vegetalization cosmetic has become world today's innovation and development
Trend, the common concern of each pharmacy big country of the world is screened effective ingredient from natural plants and is made up for development of new beauty and skin care
Product.Therefore verify Chinese herbal medicine extracting, the mechanism of isolation technics, Optimized Extraction Process parameter, specify application prospect etc. and be increasingly becoming
Beauty and skin care Cosmetic Manufacture and the key content of research.Owing to various crude drug cell structures are different, the difference of ingredient with
And extraction process differs, often need to extract Properties of Objects according to difference and select suitable extraction separation method.At present in sky
So in the extracting method of product effective ingredient, conventional traditional extraction process such as infusion process, steam or hydrodistillation, milling process,
The extraction efficiency such as THROUGH METHOD and surname extraction is the highest, loss of effective components is many, cycle length, operation are many.Some novel extractive techniques
As ultrasonic assistant extracts (ultrasound-assisted extraction, UAE), supercritical fluid extraction
(SupercriticalFluidExtraction, SFE), accelerated solvent extraction (pressurized liquid
Extraction, PLE), microwave radiation exaraction (microwave-assisted extraction, MAE), etc. the application of technology
More and more extensive.But said method is typically limited to be applied to the extraction of single Chemical Constituents of Chinese Traditional And Folk Medicine.These extracting method
For the suitability of compound Chinese herb and optimization of operating condition and extraction mechanism of mass transmission research, pilot scale and commercial scale plant
Research and development etc. are current problem demanding prompt solutions.
Summary of the invention
In order to overcome whitening sunlight screening skin-protecting product whitening sun protection weak effect in prior art, cost congruence high, uneasy not enough with
Shortcoming, the primary and foremost purpose of the present invention is to provide a kind of whitening sun protection skin care compositions.
Another object of the present invention is to provide the preparation method of above-mentioned whitening sun protection skin care compositions.
It is still another object of the present invention to provide the application of above-mentioned whitening sun protection skin care compositions.
The purpose of the present invention is achieved through the following technical solutions:
A kind of whitening sun protection skin care compositions, is prepared by following raw material components in parts by mass:
Described whitening sun protection skin care compositions, is preferably prepared by following raw material components in parts by mass:
The preparation method of described whitening sun protection skin care compositions, comprises the steps of:
(1) Fructus Evodiae, Radix Hamamelidis Mollis, Herba Leonuri and the Pseudobulbus Bletillae (Rhizoma Bletillae) are separately dried, pulverize and sieve, obtain Fructus Evodiae coarse powder, gold thread
Prunus mume (sieb.) sieb.et zucc. coarse powder, Herba Leonuri coarse powder and Pseudobulbus Bletillae (Rhizoma Bletillae) coarse powder；
(2) Fructus Evodiae coarse powder, Radix Hamamelidis Mollis coarse powder and Herba Leonuri coarse powder are mixed, be placed in supercritical CO2In extraction kettle, carry out
CO2Supercritical fluid extraction；Wherein, Extracting temperature is 40～50 DEG C, extract pressure be 30～40Mpa, extraction time be 2～
3h, CO2Flow velocity is 15～20L/h, and separation temperature is 40～50 DEG C, and separating pressure is 5～10Mpa；Obtain extract one；
(3) plant residue after step (2) being extracted is placed in supercritical CO2In extraction kettle, carry out secondary CO2Shooting flow
Body extracts；Wherein, ethanol is as entrainer, and Extracting temperature is 40～50 DEG C, and extracting pressure is 20～30Mpa, and extraction time is 2
～3h, CO2Flow velocity is 10～15L/h, and separation temperature is 40～50 DEG C, and separating pressure is 5～10Mpa；Obtain extract two；
(4) plant residue after step (3) being extracted mixes with ethanol water, inserts in high-pressure bottle, at normal temperatures
Apply 600～800MPa pressure to extract, pressurize 10～20min, filter after release, filtrate is concentrated, obtains extract three；
(5) being mixed with water by Pseudobulbus Bletillae (Rhizoma Bletillae) coarse powder and carry out microwave radiation exaraction, wherein, extracting power is 500～600W, solid-to-liquid ratio
For (1:3)～(1:20), extraction time is 2～4 times, and each extraction time is 8～15min, merging filtrate, concentrates, is carried
Take thing four；
(6) extract mix homogeneously step (2), (3), (4) and (5) prepared, obtains whitening sun protection skin care compositions；
10～40 mesh sieves that sieved preferably described in step (1)；
CO described in step (2)2The condition of supercritical fluid extraction is preferably: Extracting temperature is 45 DEG C, extracts pressure
For 35Mpa, extraction time is 2.5h, CO2Flow velocity is 16L/h, and separation temperature is 50 DEG C, and separating pressure is 7Mpa；
CO described in step (3)2The condition of supercritical fluid extraction is preferably: volume fraction is the ethanol conduct of 95%
Entrainer, volume fraction is that the consumption of the ethanol of 95% is equivalent to quality of material 8% in extraction kettle, and Extracting temperature is 48 DEG C, carries
Pressure power is 25Mpa, and extraction time is 2.5h, CO2Flow velocity is 12L/h, and separation temperature is 48 DEG C, and separating pressure is 8Mpa；
The solid-to-liquid ratio of the extraction described in step (4) is preferably (1:3)～(1:20)；
The condition of the extraction described in step (4) is preferably 700MPa pressurize 15min；
In ethanol water described in step (4), ethanol content is 50%～70% (v/v)；
The relative density of the extract three described in step (4) is 1.10～1.20；
The condition of the microwave radiation exaraction described in step (5) is preferably: extraction power is 580W, and solid-to-liquid ratio is 1:5, carries
Taking number of times is 3 times, and each extraction time is 10min；
The relative density of the extract four described in step (5) is 1.10～1.20；
Described whitening sun protection skin care compositions is applied in skin care field；
In described skin care item, the consumption of whitening sun-proof skin care composition is 0.01～10% (w/w), preferable amount be 1～
5% (w/w)；
In described skin care item, in addition to above-mentioned required composition, can coordinate as suitably desired generally at cosmetics
The composition of middle use, such as, the matrix components such as wetting agent, antioxidant, surfactant, alcohols, water；
The dosage form of described skin care item is preferably solution, suspension, emulsion, cream, paste, gel, milk, skin protection
Liquid, powder, fancy soap, oil, dry powder, foundation emulsion, wet-milling or spray；
The principle of the present invention:
Fructus Evodiae has the effects such as dispersing cold for relieving pain, the soothing the liver therapeutic method to keep the adverse QI flowing downwards, warming middle-JIAO dampness, includes volatile oil, alkaloid, polysaccharide, ammonia
The plurality of active ingredients such as base acid and flavone, have good sun-proof performance, and have bacteriostasis UVA and UVB；In Radix Hamamelidis Mollis
Containing multiple tannin matter, sebum secretion can be regulated, there is moisturizing and Milk Protein, lymph blood circulation can be promoted, can special gram
Take eye bladder in morning and black eye, the effect there is calmness, pacifying, be full of cracks, sunburn, acne are had and improves effect；Herba Leonuri extracts
Thing has the function of promoting blood flow to regulate menstruation, promoting tissue regeneration by removing blood stasis, inducing diuresis to remove edema, heat-clearing and toxic substances removing, and tyrosinase activity and B-16 can be suppressed black
The propagation of element oncocyte；The Pseudobulbus Bletillae (Rhizoma Bletillae) contains abundant Pseudobulbus Bletillae (Rhizoma Bletillae) colloid, and it is mainly composed of Pseudobulbus Bletillae polysaccharose, it is possible to be effectively prevented skin
Chilblain and chapping, has blood circulation promoting and improves the effects such as skin-nourishing situation, and the present invention is reasonably joined by each raw material
5, use CO2Lipotropy, relative molecular mass in supercritical liquid extraction technique extraction Fructus Evodiae, Radix Hamamelidis Mollis, Herba Leonuri are little
Material, the material big for the polarity in Fructus Evodiae, Radix Hamamelidis Mollis, Herba Leonuri, relative molecular mass is big, then add ethanol and carry agent
Again carry out CO2Supercritical fluid extraction and extracting the most further, makes having in Fructus Evodiae, Radix Hamamelidis Mollis, Herba Leonuri
Effect skin care ingredient maximizes the use.For the Pseudobulbus Bletillae (Rhizoma Bletillae), then use its Pseudobulbus Bletillae (Rhizoma Bletillae) colloid of microwave radiation exaraction, there is selectivity
Height, rate of extraction are fast, the biological of favorable reproducibility, time-saving energy-saving, extraction ratio height, good product quality, survivable Pseudobulbus Bletillae polysaccharose is lived
Property with the feature such as chemical constitution.The present invention passes through reasonable compatibility, makes different effective active compositions produce synergistic function, pole
The big property of medicine exciting effective active matter, the whitening sun protection skin care compositions prepared have sun-proof, whitening, speckle dispelling,
The effects such as antiallergic, antibacterial, moisturizing.
The present invention has such advantages as relative to prior art and effect:
(1) present invention provide whitening sun protection skin care compositions can be effectively prevented intensive ultraviolet to skin radiate, permissible
Suppression tyrosinase activity and the propagation of B-16 melanoma cells, have sun-proof whitening and spot-removal function.
(2) the whitening sun protection skin care compositions that the present invention provides can help skin to eliminate sensitive response, plays reduction
Stimulate, eliminate edema and the effect of red blood streak.
(3) the whitening sun protection skin care compositions that the present invention provides is rich in materials such as tannins, has the strongest convergence, also may be used
To solidify the protoplasm of microorganism, there is certain antibacterial action.
(4) present invention passes through reasonable compatibility, makes different effective active compositions produce synergistic function, excites greatly
The property of medicine of effective active matter.
(5) present invention uses different extracting method to extract compound raw material so that in composition material
Effective skin care ingredient maximize the use.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited to this.
Embodiment 1
(1) 12 mass parts Fructus Evodiaes, 7 mass parts Radix Hamamelidis Molliss, 10 mass parts Herba Leonuris and the 6 mass parts Pseudobulbus Bletillae (Rhizoma Bletillae)s are done respectively
Dry, pulverized 20 mesh sieves, obtain Fructus Evodiae coarse powder, Radix Hamamelidis Mollis coarse powder, Herba Leonuri coarse powder and Pseudobulbus Bletillae (Rhizoma Bletillae) coarse powder；
(2) Fructus Evodiae coarse powder, Radix Hamamelidis Mollis coarse powder and Herba Leonuri coarse powder are mixed, be placed in supercritical CO2In extraction kettle, carry out
CO2Supercritical fluid extraction；Wherein, Extracting temperature is 45 DEG C, and extraction pressure is 35Mpa, and extraction time is 2.5h, CO2Flow velocity is
16L/h, separation temperature is 50 DEG C, and separating pressure is 7Mpa；Obtain extract one；
(3) plant residue after step (2) being extracted is placed in supercritical CO2In extraction kettle, carry out secondary CO2Shooting flow
Body extracts；Wherein, volume fraction be the ethanol of 95% as entrainer, volume fraction is that the consumption of the ethanol of 95% is equivalent to extraction
Taking quality of material 8% in still, Extracting temperature is 48 DEG C, and extraction pressure is 25Mpa, and extraction time is 2.5h, CO2Flow velocity is 12L/
H, separation temperature is 48 DEG C, and separating pressure is 8Mpa；Obtain extract two；
(4) plant residue after step (3) being extracted and ethanol content are the ethanol water (solid-to-liquid ratio of 60% (v/v)
For 1:5) mixing, insert in high-pressure bottle, apply 700MPa pressure at normal temperatures and extract, pressurize 15min, mistake after release
Filter, concentrating the filtrate to relative density is 1.20, obtains extract three；
(5) being mixed with water by Pseudobulbus Bletillae (Rhizoma Bletillae) coarse powder and carry out microwave radiation exaraction, wherein, extraction power is 580W, and solid-to-liquid ratio is 1:
5, extraction time is 3 times, and each extraction time is 10min, merging filtrate, and being concentrated into relative density is 1.20, obtains extract
Four；
(6) extract mix homogeneously step (2), (3), (4) and (5) prepared, obtains whitening sun protection skin care compositions.
Embodiment 2
(1) 10 mass parts Fructus Evodiaes, 5 mass parts Radix Hamamelidis Molliss, 9 mass parts Herba Leonuris and the 4 mass parts Pseudobulbus Bletillae (Rhizoma Bletillae)s are separately dried,
Pulverize 40 mesh sieves, obtain Fructus Evodiae coarse powder, Radix Hamamelidis Mollis coarse powder, Herba Leonuri coarse powder and Pseudobulbus Bletillae (Rhizoma Bletillae) coarse powder；
(2) Fructus Evodiae coarse powder, Radix Hamamelidis Mollis coarse powder and Herba Leonuri coarse powder are mixed, be placed in supercritical CO2In extraction kettle, carry out
CO2Supercritical fluid extraction；Wherein, Extracting temperature is 40 DEG C, and extraction pressure is 40Mpa, and extraction time is 3h, CO2Flow velocity is
20L/h, separation temperature is 45 DEG C, and separating pressure is 10Mpa；Obtain extract one；
(3) plant residue after step (2) being extracted is placed in supercritical CO2In extraction kettle, carry out secondary CO2Shooting flow
Body extracts；Wherein, volume fraction be the ethanol of 95% as entrainer, volume fraction is that the consumption of the ethanol of 95% is equivalent to extraction
Taking quality of material 5% in still, Extracting temperature is 40 DEG C, and extraction pressure is 30Mpa, and extraction time is 3h, CO2Flow velocity is 15L/h,
Separation temperature is 40 DEG C, and separating pressure is 10Mpa；Obtain extract two；
(4) plant residue after step (3) being extracted and ethanol content are the ethanol water (solid-to-liquid ratio of 50% (v/v)
For 1:3) mixing, insert in high-pressure bottle, apply 600MPa pressure at normal temperatures and extract, pressurize 20min, mistake after release
Filter, concentrating the filtrate to relative density is 1.10, obtains extract three；
(5) being mixed with water by Pseudobulbus Bletillae (Rhizoma Bletillae) coarse powder and carry out microwave radiation exaraction, wherein, extraction power is 600W, and solid-to-liquid ratio is 1:
3, extraction time is 2 times, and each extraction time is 8min, merging filtrate, and being concentrated into relative density is 1.10, obtains extract
Four；
(6) extract mix homogeneously step (2), (3), (4) and (5) prepared, obtains whitening sun protection skin care compositions.
Embodiment 3
(1) 15 mass parts Fructus Evodiaes, 9 mass parts Radix Hamamelidis Molliss, 12 mass parts Herba Leonuris and the 9 mass parts Pseudobulbus Bletillae (Rhizoma Bletillae)s are done respectively
Dry, pulverized 10 mesh sieves, obtain Fructus Evodiae coarse powder, Radix Hamamelidis Mollis coarse powder, Herba Leonuri coarse powder and Pseudobulbus Bletillae (Rhizoma Bletillae) coarse powder；
(2) Fructus Evodiae coarse powder, Radix Hamamelidis Mollis coarse powder and Herba Leonuri coarse powder are mixed, be placed in supercritical CO2In extraction kettle, carry out
CO2Supercritical fluid extraction；Wherein, Extracting temperature is 50 DEG C, and extraction pressure is 30Mpa, and extraction time is 2h, CO2Flow velocity is
15L/h, separation temperature is 50 DEG C, and separating pressure is 5Mpa；Obtain extract one；
(3) plant residue after step (2) being extracted is placed in supercritical CO2In extraction kettle, carry out secondary CO2Shooting flow
Body extracts；Wherein, volume fraction be the ethanol of 95% as entrainer, volume fraction is that the consumption of the ethanol of 95% is equivalent to extraction
Taking quality of material 10% in still, Extracting temperature is 50 DEG C, and extraction pressure is 20Mpa, and extraction time is 2h, CO2Flow velocity is 10L/
H, separation temperature is 50 DEG C, and separating pressure is 5Mpa；Obtain extract two；
(4) plant residue after step (3) being extracted and ethanol content are the ethanol water (solid-to-liquid ratio of 70% (v/v)
For 1:20) mixing, insert in high-pressure bottle, apply 800MPa pressure at normal temperatures and extract, pressurize 10min, mistake after release
Filter, it is 1.15 that filtrate concentrates relative density, obtains extract three；
(5) being mixed with water by Pseudobulbus Bletillae (Rhizoma Bletillae) coarse powder and carry out microwave radiation exaraction, wherein, extraction power is 500W, and solid-to-liquid ratio is 1:
20, extraction time is 4 times, and each extraction time is 15min, merging filtrate, and being concentrated into relative density is 1.15, obtains extract
Four；
(6) extract mix homogeneously step (2), (3), (4) and (5) prepared, obtains whitening sun protection skin care compositions.
Effect example
(1) respectively the whitening sun protection skin care compositions that embodiment 1～3 prepares being configured to concentration with dimethyl sulfoxide is
The active ingredient solution of mass volume ratio 1%, carries out following experiment:
(1) anti-ultraviolet property measures
In 280～400nm wave-length coverages, it is scanned every 10nm, determines the saturating of active ingredient solution under each wavelength
Light rate, calculates the average absorption ratio of wave-length coverage.
(2) tyrosine vitality test in suppression mouse melanin cell
The B16 mouse melanin cell (Shanghai cell institute of the Chinese Academy of Sciences) being in exponential phase is inoculated in 96 orifice plates,
Culture medium is the calf serum of RPMI1640+ percent by volume 10%, every hole 100 μ L (104Individual cells/well), put 37 DEG C, 5%
CO2After incubator cultivates 24h, add the active ingredient solution 50 μ L after filtration sterilization.After continuing to cultivate 3d, supernatant discarded
Liquid, rinses twice with 0.15% (w/v) poly butylene succinate that pH value is 6.8, and every hole adds 90 μ L containing 1% (volume fraction)
The PBS (0.01M, pH value 7.4) of Triton X-100.Ultrasonication in ice bath, every hole adds the L-3,4 dihydroxyphenylalanine of 10 μ L 10mmol/L
(L-dopa), hatching 60min for 37 DEG C, colorimetric at 475nm, blank well returns to zero, surveys each hole absorbance.
Suppression ratio=(1-medicine group mean absorbance values ÷ matched group mean absorbance values) × 100%
Interpretation of result:
(1) anti-ultraviolet property measures, and result is as shown in table 1:
The whitening sun protection skin care compositions anti-ultraviolet property that table 1 embodiment 1～3 prepares measures
| Embodiment | 280～320nm absorbances (%) | 320～400nm absorbances (%) |
| Embodiment 1 | 100 | 95.6 |
| Embodiment 2 | 100 | 93.5 |
| Embodiment 3 | 100 | 91.2 |
(2) tyrosine vitality test in suppression mouse melanin cell, result is as shown in table 2:
Tyrosine vitality test result in table 2 embodiment 1～3 suppression mouse melanin cell
Application Example
Take the whitening sun protection skin care compositions of 5% (w/w), the glycerol of 15% (w/w), the sorbitol of 10% (w/w) respectively
Trioleate, the purified water of 65% (w/w) make whitening sun protection cosmetic emulsion.
The whitening sun protection cosmetic emulsion prepared is carried out clinic trial observation.
The object of observation: age 20～45 years old middle-aged women.Choosing face has chloasma in various degree, butterfly spot, senile plaque
Female middle-aged 100 example Deng pigment.
Observational technique: before sleep every night after cleaning skin, coat above-mentioned whitening sun protection cosmetic emulsion.Use continuously
It within 28 days, it is a course for the treatment of.
Criterion of therapeutical effect: (1) is effective: melanin disappears and reaches more than 90%, skin has the most moist smooth feeling；(2) effective:
Melanin disappears more than 50%, and skin is relatively moistened smooth；(3) invalid: unchanged before and after treatment.The results are shown in Table 3.
Table 3 normal individual trial experiment effective percentage result
| Number of cases | Effective | Effectively | Invalid | Total effective rate | |
| Embodiment 1 | 100 | 94 | 3 | 3 | 97% |
| Embodiment 2 | 100 | 92 | 4 | 4 | 96% |
| Embodiment 3 | 100 | 91 | 4 | 5 | 95% |
Above-described embodiment is the present invention preferably embodiment, but embodiments of the present invention are not by above-described embodiment
Limit, the change made under other any spirit without departing from the present invention and principle, modify, substitute, combine, simplify,
All should be the substitute mode of equivalence, within being included in protection scope of the present invention.
Claims (10)
1. a whitening sun protection skin care compositions, it is characterised in that prepared by following raw material components in parts by mass:
Whitening sun protection skin care compositions the most according to claim 1, it is characterised in that by following raw material in parts by mass
Component prepares:
3. the preparation method of the whitening sun protection skin care compositions described in claim 1 or 2, it is characterised in that comprise the steps of:
(1) Fructus Evodiae, Radix Hamamelidis Mollis, Herba Leonuri and the Pseudobulbus Bletillae (Rhizoma Bletillae) are separately dried, pulverize and sieve, obtain Fructus Evodiae coarse powder, Radix Hamamelidis Mollis thick
Powder, Herba Leonuri coarse powder and Pseudobulbus Bletillae (Rhizoma Bletillae) coarse powder；
(2) Fructus Evodiae coarse powder, Radix Hamamelidis Mollis coarse powder and Herba Leonuri coarse powder are mixed, be placed in supercritical CO2In extraction kettle, carry out CO2Super
Supercritical fluid extraction；Wherein, Extracting temperature is 40～50 DEG C, and extracting pressure is 30～40Mpa, and extraction time is 2～3h, CO2
Flow velocity is 15～20L/h, and separation temperature is 40～50 DEG C, and separating pressure is 5～10Mpa；Obtain extract one；
(3) plant residue after step (2) being extracted is placed in supercritical CO2In extraction kettle, carry out secondary CO2Supercritical fluid extracts
Take；Wherein, ethanol is as entrainer, and Extracting temperature is 40～50 DEG C, extract pressure be 20～30Mpa, extraction time be 2～
3h, CO2Flow velocity is 10～15L/h, and separation temperature is 40～50 DEG C, and separating pressure is 5～10Mpa；Obtain extract two；
(4) plant residue after step (3) being extracted mixes with ethanol water, inserts in high-pressure bottle, applies at normal temperatures
600～800MPa pressure extract, pressurize 10～20min, filter after release, filtrate are concentrated, obtain extract three；
(5) being mixed with water by Pseudobulbus Bletillae (Rhizoma Bletillae) coarse powder and carry out microwave radiation exaraction, wherein, extracting power is 500～600W, and solid-to-liquid ratio is
(1:3)～(1:20), extraction time is 2～4 times, and each extraction time is 8～15min, merging filtrate, concentrates, is extracted
Thing four；
(6) extract mix homogeneously step (2), (3), (4) and (5) prepared, obtains whitening sun protection skin care compositions.
The preparation method of whitening sun protection skin care compositions the most according to claim 3, it is characterised in that:
CO described in step (2)2The condition of supercritical fluid extraction is: Extracting temperature is 45 DEG C, and extraction pressure is 35Mpa, carries
The time of taking is 2.5h, CO2Flow velocity is 16L/h, and separation temperature is 50 DEG C, and separating pressure is 7Mpa.
The preparation method of whitening sun protection skin care compositions the most according to claim 3, it is characterised in that:
CO described in step (3)2The condition of supercritical fluid extraction is: volume fraction is that the ethanol of 95% is as entrainer, body
Fraction is that the consumption of the ethanol of 95% is equivalent to quality of material 8% in extraction kettle, and Extracting temperature is 48 DEG C, extracts pressure and is
25Mpa, extraction time is 2.5h, CO2Flow velocity is 12L/h, and separation temperature is 48 DEG C, and separating pressure is 8Mpa.
The preparation method of whitening sun protection skin care compositions the most according to claim 3, it is characterised in that:
The condition of the extraction described in step (4) is 700MPa pressurize 15min；
In ethanol water described in step (4), ethanol content is 50%～70% (v/v).
The preparation method of whitening sun protection skin care compositions the most according to claim 3, it is characterised in that:
The condition of the microwave radiation exaraction described in step (5) is: extraction power is 580W, and solid-to-liquid ratio is 1:5, and extraction time is
3 times, each extraction time is 10min.
The preparation method of whitening sun protection skin care compositions the most according to claim 3, it is characterised in that:
The relative density of the extract three described in step (4) is 1.10～1.20；
The relative density of the extract four described in step (5) is 1.10～1.20.
9. the whitening sun protection skin care compositions described in claim 1 or 2 is applied in skin care field.
Whitening sun protection skin care compositions the most according to claim 9 is applied in skin care field, it is characterised in that:
The dosage form of described skin care item is solution, suspension, emulsion, cream, paste, gel, milk, skin care liquid, powder, perfume (or spice)
Soap, oil, dry powder, foundation emulsion, wet-milling or spray.
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Application publication date: 20170104 |