CN112365920A - 一种鉴定蜜蜂分化关键基因的方法及鉴定得到的基因和应用 - Google Patents
一种鉴定蜜蜂分化关键基因的方法及鉴定得到的基因和应用 Download PDFInfo
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Abstract
本发明涉及一种鉴定蜜蜂分化关键基因的方法及鉴定得到的基因和应用。所述方法包括获得多个蜜蜂96h幼虫的全基因组信息，通过三维基因组技术进行分析得到多个蜜蜂基因拓扑相关结构域，根据蜂王浆主蛋白家族成员在所述多个蜜蜂基因拓扑相关结构域的分布确定和蜜蜂分化相关的关键基因。本发明以蜂王浆主蛋白家族成员在蜜蜂基因拓扑相关结构域中的分布为基础，通过三维基因技术分析得到多个与蜜蜂96h幼虫级型分化密切相关的蛋白及其编码基因。本发明发现这些与蜜蜂级型分化相关的蛋白在蜂王和工蜂幼虫级型分化阶段表达水平差异显著，对养蜂过程中96h幼虫分化提供了基因水平上的依据，对于诱导96h幼虫分化为蜂王或工蜂具有重要指导意义。
Description
技术领域
本发明涉及蜜蜂养殖技术领域，尤其涉及一种鉴定蜜蜂分化关键基因的方法及鉴定得到的基因和应用。
背景技术
蜜蜂属于膜翅目，蜜蜂科，是维持生态环境持续发展不可或缺的物种，是植物、特别是新兴的设施作物的主要授粉昆虫。
蜜蜂是社会化昆虫，有级型分化现象。级型分化是蜜蜂生殖、劳动分工的基础。在正常蜂群中，通常有一只蜂王，数百、数千、甚至数万只工蜂。蜂王和工蜂都是雌性蜂，由遗传物质完全相同的受精卵发育而来，但是蜂王和工蜂在生理、行为方面差异很大。相对于工蜂，蜂王口器退化、体型更大、腹部更长，腿部没有花粉筐，蛰针更短，无咽下腺，下颌腺更发达，发育周期比工蜂提前5天。蜂王有150～200根卵巢管，在蜂群中担任繁殖后代的任务。工蜂只有1～20根卵巢管，正常情况下不具备繁殖能力。蜂王的自然寿命可达5～6年。工蜂的寿命，在采集季节只有5～6个月。在自然蜂群中，交配之后的蜂王就专职产卵并通过蜂王信息素调控蜂群成员的行为和生理变化。工蜂在蜂群中承担保温孵育、清理蜂房、调剂花粉与蜂蜜、饲喂幼虫及蜂王、分泌王浆、蜜蜡造脾、采集花蜜、花粉和蜂胶及巢门防卫等任务。
96h幼虫是蜜蜂级型分化的关键时期。96h工蜂幼虫移至王台可发育成蜂王，大于96h的工蜂幼虫在王台中培育只能发育成工蜂。近几年，研究人员对蜜蜂96h幼虫的RNA进行了研究，利用两两差异比较的方法，得到了96h蜂王和工蜂幼虫中表达水平发生改变的基因，认为这些基因与蜜蜂幼虫级型分化有关。但是这种方法因为主观上对差异表达的显著性的设置，而出现假阳性或假阴性的结果，使得真正影响级型分化的基因被漏掉，或者得到许多并不是真正影响级型分化的基因。
发明内容
为了至少解决一种现有技术存在的问题，本发明提供一种鉴定蜜蜂分化关键基因的方法及鉴定得到的基因和应用。本发明通过三维基因组技术，以蜂王浆主蛋白家族成员为依据，鉴定得到多个与蜜蜂分化密切相关的基因，其在蜂王和工蜂幼虫级型分化阶段表达水平差异显著。
鉴定蜜蜂级型分化关键基因对揭示蜜蜂级型分化分子机制有重要意义，为调控蜜蜂级型分化奠定基础。因此，准确、客观的鉴定蜜蜂级型分化的关键基因十分重要。
据此，第一方面，本发明提供一种鉴定蜜蜂分化关键基因的方法，包括：
获得多个蜜蜂96h幼虫的全基因组信息，通过三维基因组技术进行分析得到多个蜜蜂基因拓扑相关结构域，根据蜂王浆主蛋白家族成员在所述多个蜜蜂基因拓扑相关结构域的分布确定和蜜蜂分化相关的关键基因。
进一步地，所述蜂王浆主蛋白家族成员包括：所述蜂王浆主蛋白家族成员包括：MRJP1、MRJP2、MRJP3、MRJP4、MRJP5、MRJP6、MRJP7、MRJP8和MRJP9。蜂王浆主蛋白(Majorroyal jelly proteins,MRJPs)家族主要有9个成员，其中MRJP1/7是弱酸性蛋白，水溶性蛋白。MRJP2、3、4、5、6、8、9是弱碱性蛋白
进一步地，所述获得多个蜜蜂96h幼虫的全基因组信息为：
获得多个蜜蜂96h幼虫的组织，经过预处理、酶切、生物素标记、平末端连接及DNA纯化提取后得到Hi-C样本，使用所述Hi-C样本建立蜜蜂全基因信息文库并测序用于后续的三维基因组技术分析。
更进一步，所述预处理为将所述组织置于1％～3％的甲醛DMEM培养基中进行交联处理，后加入甘氨酸溶液混匀反应充分后离心去上清。
进一步地，所述通过三维基因组技术进行分析得到多个蜜蜂基因拓扑相关结构域为使用80～100kb的分辨率对染色体进行bin的划分，后通过Insulation算法或directionality index算法鉴定TAD的边界得到多个蜜蜂基因拓扑相关结构域。
进一步地，所述确定和蜜蜂分化相关的关键基因为：
统计蜂王浆主蛋白家族成员在所有拓扑相关结构域中的分布，确定所述蜂王浆主蛋白家族成员最多的拓扑相关结构域中的基因为和蜜蜂分化相关的关键基因。
目前，基于线性DNA序列的测序技术仍难以明确整合蜜蜂级型分化发生发展的机制。因此本发明假设蜜蜂级型分化发生阶段基因组三维空间结构发生变化，可能导致基因组原有的拓扑相关结构域(Topologically associated domains，TAD)的破坏以及基因之间交互发生改变，从而导致基因组上在线性距离相距较远但在三维结构相互靠近交互强烈的相关基因及通路的调控发生改变，这些基因及基因通路是与级型分化的发生密切相关。
因此本发明应用Hi-C技术，可以克服以往测序仅从线性水平对级型分化发生过程中基因组变化进行解读的缺点，能从更高层次地三维基因组水平来研究蜜蜂级型分化过程中基因组空间的改变，对级型分化的发生机制进行更深入的研究。
本发明进一步提供所述方法在辅助蜜蜂级型分化中的应用。
本发明进一步提供L-木酮糖还原酶、LOC724335蛋白或LOC724286蛋白在鉴定分化为蜂王或者工蜂的蜜蜂幼虫应用，所述L-木酮糖还原酶含有如SEQ ID NO:1所示的氨基酸序列，所述LOC724286蛋白含有如SEQ ID NO:3所示的氨基酸序列，所述LOC724335蛋白含有如SEQ ID NO:5和SEQ ID NO:6所示的氨基酸序列。
进一步地，所述应用为：
对蜜蜂幼虫的L-木酮糖还原酶进行检测，L-木酮糖还原酶表达量高的分化为蜂王，L-木酮糖还原酶表达量低的分化为工蜂；和/或，
对蜜蜂幼虫的LOC724286蛋白进行检测，LOC724286蛋白表达量高的分化为工蜂，LOC724335蛋白表达量低的分化为蜂王；和/或，
对蜜蜂幼虫的LOC724335蛋白进行检测，LOC724335蛋白表达量高的分化为蜂王，表达量低的分化为工蜂。
本发明进一步提供L-木酮糖还原酶、LOC724286蛋白或LOC724335蛋白在调控蜜蜂分化中的应用。
编码所述L-木酮糖还原酶的基因包括如SEQ ID NO:2所示的核苷酸序列，和/或，编码所述LOC724286蛋白的基因包括如SEQ ID NO:4所示的核苷酸序列，和/或，编码所述LOC724335蛋白的基因包括如SEQ ID NO:7所示的核苷酸序列。
本发明具备如下有益效果：
本发明通过三维基因组技术对蜜蜂96h幼虫的基因组信息进行统计分析，并划分为多个TAD，后根据蜂王浆主蛋白家族成员在所有TAD中的分布，确定多个和蜜蜂96h幼虫分化相关的关键基因。本发明经实验验证，分析得到的L-木酮糖还原酶、LOC724335蛋白或LOC102654673蛋白在蜂王和工蜂幼虫级型分化阶段表达水平差异显著，对于诱导96h幼虫分化为蜂王或工蜂具有重要指导意义。
具体实施方式
以下实施例用于说明本发明，但不用来限制本发明的范围。
实施例1
1、样品采集：采集蜜蜂蜂王和工蜂新鲜96h幼虫样品；
2、样品交联：
(1)将新鲜手术样本放入装有>20mL冰PBS的50mL离心管中，转移至具备通风橱或生物安全柜的实验室；
(2)在冰PBS溶液中涮洗组织样本，使用无尘纸吸取组织表面的PBS液体；
(3)将吸干的组织样本放入无菌的培养皿中，使用移液器吸头或注射器吸头，将组织样本机械切割，每1.5mL离心管装100mg切割后的样本；
(4)向1.5mL离心管中加入1mL的2％甲醛DMEM培养基，将离心管置于混匀仪上旋转，或者平置在生物安全柜桌面；
(5)室温精确交联10min，在2.5min、5Min和7.5min时，颠倒混匀，保证交联充分；
(6)交联到10min后，立刻加入108uL的2.5M甘氨酸溶液，颠倒混匀，培养基会由粉红色变为亮黄色；
(7)将离心管置于混匀仪上中和5min，在2.5min时，颠倒混匀一次；
(8)中和到5min后，立刻将离心管转移至冰上；
(9)冰上终止至少15min，保证终止反应彻底；
(10)4℃离心机800g离心10min，离心结束后缓慢弃掉上清，注意避免将组织颗粒弃掉；
(11)加入1mL冰PBS溶液充分清洗组织颗粒，4℃离心机800g离心10min；
(12)离心结束后，使用1mL移液器缓慢吸弃上清，然后使用200uL移液器充分去除离心管中的残留液；
(13)使用封口膜密封离心管口，液氮速冻后-80℃冰箱储存，以备后用。
3、HiC片段制备流程：使用限制性内切酶(HindIII/MboI)进行染色质消化，并取样检测酶切效果。之后经生物素标记、平末端连接及DNA纯化提取，制备Hi-C样本，并取样进行DNA质量检测。检测合格后进入标准文库构建流程。Hi-C片段经去除末端标记的生物素，超声打断，末端修复，加碱基A，钓取含有生物素的片段，加测序接头形成加接头产物，之后进行PCR条件的筛选并扩增获得文库产物。
构建好的文库经过文库质控合格后，使用IlluminaPlatform进行测序，测序策略一般使用PE150。
4、数据分析：对原始下机数据过滤，获得高质量的Reads。本实施例使用HiC-Pro的比对策略，调用bowtie2进行数据比对比对到基因组，参考基因组为apis melliferareference genome(4.5)。
5、TAD分析：本实施例使用100kb的分辨率将染色体划分成2205个bin，之后选用Insulation算法鉴定TAD的边界。
6、蜜蜂级型分化相关TAD的筛选：TAD为基因精细表达调控提供合适的微环境。TAD内部相互作用频率非常高，但不同TAD之间即使线性距离非常靠近，相互作用也非常弱。蜂王浆主蛋白是目前发现并公认的在蜜蜂级型分化过程中发挥主要作用的基因。
本实施例发现蜂王浆主蛋白家族成员主要集中在一个TAD内，这个TAD位于染色体的(NC_007080.3:2540000-2700000)位置处。根据TAD的性质，本实施例将这个TAD作为蜜蜂级型分化的关键TAD。
7、蜜蜂级型分化关键基因的鉴定：在目标TAD内部共发现了20个转录本。其中，15个基因是蜂王浆主蛋白家族成员，2个是未知的蛋白(LOC724335蛋白和LOC724286蛋白)，1个是非编码RNA，还有1个是L-xylulose reductase(gene id:413567)。因此，本实施例确定L-xylulose reductase(gene id:413567)、LOC724335和LOC724286是影响蜜蜂级型分化的关键基因。
8、蜜蜂级型分化关键基因的验证：本实施例采用转录组测序的方法对这21个基因进行表达水平的验证，发现L-xylulose reductase(gene id:413567)、LOC724335和LOC724286在蜂王和工蜂中幼虫级型分化阶段表达水平差异显著。
表1目标TAD中各转录组的信息
虽然，上文中已经用一般性说明及具体实施方案对本发明作了详尽的描述，但在本发明基础上，可以对之作一些修改或改进，这对本领域技术人员而言是显而易见的。因此，在不偏离本发明精神的基础上所做的这些修改或改进，均属于本发明要求保护的范围。
序列表
<110> 中国农业科学院蜜蜂研究所
<120> 一种鉴定蜜蜂分化关键基因的方法及鉴定得到的基因和应用
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Val Asn Pro Thr Val Ile Leu Thr Glu Met Gly Lys Leu Gly Trp Ser
180 185 190
Asp Pro Lys Lys Ala Arg Thr Met Leu Asp Lys Ile Pro Leu Gly Arg
195 200 205
Phe Gly Glu Val Ser Glu Val Val Asp Ala Ile Val Tyr Leu Leu Ser
210 215 220
Asn His Ser Ser Met Ile Asn Gly Ile Thr Leu Pro Val Asp Gly Gly
225 230 235 240
Phe Leu Ala Thr
<210> 2
<211> 3328
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 2
agatagttga tggttattgg tggggttaaa tggaataaat atatttaaca agcaatattt 60
cacagtagtt gataacgcat tcgtagccat gaacatcaat tttgtgggaa aacgtattct 120
tgtaacagga gctggacgag gtatgttata tatttgatat tatatcataa ttcagtaaac 180
tttctttcta tttaaatttt aattgttgat aaagataatt attaaatatt attttaaatt 240
cttttctatt ttaaatccac attgtttatt cacatttctt tattgagaaa gaatttaaat 300
tgaaccaatc agaaagagct atatattttt tgtttgataa aagttggtta tgtaataata 360
tttatcttga aatacaataa ttccattgaa aacaattttt catgaatata ttgtgatatt 420
tgtaaaaaat ttcgattata ttaattctat taattatatt aatcataaat aattatatta 480
attatgatta taatataact ataatatgat tataatatga ttataatata aatgtaatta 540
aatcataaat ttataagatt cgtattatga gtcagattta tctgaatgtt tcaatattta 600
ttttttttat tttttaatat ctttctaata aatattctta taaaagaata tctatctctt 660
cttttataat tgtttttaat tatcttacat ttcatttaca aaatatttca aattattata 720
ttttaaattg ataagtattt caactctatc atttcaattg tatcatgatt ataaatcaca 780
ttcattcata ttcatttcat gctaagtatg taattatatt cataattatt tttcttcaga 840
aaacattatc taacatttta aaacattatt taacatatta aacattttaa atcataggta 900
ttggcaaaga tttagctctt cgtctttcca aatatgaagg tcaagtaata gcgttgtcca 960
agaaaaaaga gaatctagat aagctatgta aagaagatcc tcgtattcag tttatttgcg 1020
tcgatctcag cgattggaac gccaccagga aagccgttga aagtgtgtta cctatagatc 1080
tcttagtaaa taatgccggt gttgcacatc ttaattcttt cttcgatgct acaccagaag 1140
attttgattt aacatttaca gtaaatgtga aagctatatt gaatgtttct cagatagtcg 1200
caaaaaatat gattgaaaga aaagttggtg gtagtattgt taatatttct tcgcaagcca 1260
gccaagcagc tttgaaggat catgttgtct attgcgcttc gaaaggagca gtagatatgt 1320
tgtctaagta agaagaaatc agttttttat ttatttattt atttattatt atataaataa 1380
aaaaatgaat tgctcttata acataatttt taattaagac ttaaaaaata tttaaaaact 1440
taagttatat ctatgctata atttatatat atatctatta tacgtaatct attttaccta 1500
ccttttttta acagctcata aaatttatta aataaatttc atttttaata attatttctt 1560
tccagaacaa tggctcttga gcttggtccc tataatattc gagtgaatac cgtgaatcct 1620
acagtgattt tgacggaaat gggaaaatta ggttggagtg atccgaaaaa agcacgaact 1680
atgttagaca aaataccatt aggtcgattc ggcggtaggt aatcatttat ggaatatatt 1740
tttggaatag atttctacca gtttttatta tttatattct tatattctat tttcttatat 1800
attttgcaga agtgtccgag gtagtggatg cgatagtgta tttattaagc aatcacagct 1860
cgatgattaa tggaattaca ttgccagtgg atggtggatt tttagcaaca taattgcata 1920
aatcaatatc atttattttt atatttatat tatttttata taatatatat catatattta 1980
tattatttat ataaatttta tatatttcat atttttttaa ttttagaaaa aaataatttt 2040
ataagataaa aaaatattat atacaattga aatacaattc aaataagtat ctttgcgttt 2100
aatattataa cttttgtttt acgttttgag tcttattaaa attttatcga atattgaata 2160
tatattgaat ataggagaga aaacaaggtt ttgaatgaaa agaatgaaag agaagagaga 2220
aaaaaaaaag atcaacacag ttattcgttt atctcgttta atttaaaaaa cgcttcgttt 2280
cttctttttt cattatttaa ctagaattcg gtgagcaaac ggtgagggaa cggcgttgaa 2340
acaatgaacg tctcgccgtc gaaagaaaac tcgtttattt cccgaaaata tttaaagacg 2400
agacgtgtca ttgtttcatc gaagtccccc cccatcgccg ccatttccgg tttaaaacaa 2460
acatttcgtt tttttttttg ggctctcttt ctaattcaat aattatttga atccctacag 2520
tgaacataaa gtggcggaga aaaacgcgaa attaaacaac tgcctgcaca ggtaagaaac 2580
tggtttcaaa ctgattcaga cattgttgca tctgattcga ttcacatctc ctttcgcgca 2640
tcaacgttac gagcgaagca tttccatttc caaaattaac cctttatgcc attaataata 2700
attcttaatc gttttcgcaa aaattgtttc gcagccactc gatcaaacgg gggaaaaaag 2760
attgactaaa taaaatgaaa ataaaaaaaa aaaggaataa gaaaaaagaa agcaaaaaaa 2820
aaattaacaa gtaaaacgga cgatcgcctt agtttcactt ttctcacttt ccttaagagt 2880
ttttttatat agccttctct tttgtattat ataaaaaatt aattatagac catatcaagt 2940
gtctttcgta aatttcaaaa aatacatgat tacacgttcg cagattaaga atcactattc 3000
tacgattcga tagagtacaa gtattaaaat cgattaacac gtagaaacgc ttgtttcatc 3060
ctcaatgcac cttcgtgtta ttcacgctac tcgggaatct ccaaagagta cggcggttat 3120
taaatagctg ccttattttt gattattatt attattatta ttattattat tattattatt 3180
atgttcggag aatgtcattt tgtgtacgca ctgttcttat ggcccttgtt tccatcactc 3240
atcataaagg attaacttca agacatgcat taggctatca aaagaaaaaa aaaaaaagaa 3300
aaagagagag aagaaacgga gaaaaata 3328
<210> 3
<211> 167
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 3
Met Gln Leu Thr Thr Cys Cys Lys Cys Tyr Ser Leu Lys Thr Gly Thr
1 5 10 15
Leu Phe Thr Gly Ile Leu Gly Ile Val Leu Ser Ile Ile Ser Leu Ile
20 25 30
Leu Ile Phe Thr Leu Asn Val Glu Trp Lys Thr Ile Leu Ile Asp Ile
35 40 45
Val Asp Gln Ser Ile Val Arg Ile Ile Phe Ala Ile Asn Leu Cys Met
50 55 60
Thr Ile Leu Ile Ser Thr Leu Leu Ile Val Gly Ala Leu Lys Lys Lys
65 70 75 80
Thr Phe Leu Met Leu Pro Trp Val Val Leu Gly Leu Ile Leu Ala Val
85 90 95
Gly Leu Leu Val Ser Val Leu Tyr Thr Ser Ile Met Phe Phe Ile Tyr
100 105 110
His Asp Val Ile Ile Gly Ile Leu Trp Leu Ile Ile Gly Leu Leu Thr
115 120 125
Ile Val Ile Tyr Val Tyr Leu Trp Leu Val Val Tyr Ser Tyr Phe Gln
130 135 140
Gln Leu Arg Tyr Asp Lys Met Ser Ser Arg Ile Asp Pro Tyr Gly Arg
145 150 155 160
Pro Tyr Asn Tyr Arg Arg Pro
165
<210> 4
<211> 12960
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 4
aacttcgcgg tctgccacgg cgacgtactg ttcttggcac agtttctgac catctcctat 60
tgcgagcgga tcgcgctgga cctaatatca tttgcgcctc gcgccaccca aatttctgcg 120
aggaaaccct ccgaaatctc tcttccggtt gtctgacatt tggaaaaccg cgcaccccat 180
tgcctagagg cgacatcttc gatctttcga agccaacgaa tttcctcctt tgaaagtgag 240
taaaatcgaa gaaattccgc gagttttctt ccgattttca aaaaaggtga aaaaaaatcg 300
aaagatatgg aaaatttttt ttttgatttc ttaacaaaac catttttcac aagtgaatat 360
atcgatgatt ttttaaaagt gaacaagcaa gatggaagat ttttcaaaag tgaatatatc 420
ggatggttcg gaagattttt cgaaagtgag ataaatcgaa gaagatttga aaaactttat 480
caataatttt ttcgattttt taaaagcaaa tatgtcgata gaattttgaa gattttttta 540
aaagtgaaat aaatcgaaga tttgaaaaat ttcgttagcg atttttcgat tagcgatttt 600
cttgactttt tagaaataag aagtaagtaa aaaaagaaaa aaatttttaa gatttcataa 660
ttaacgagag ttcataaaaa aaattttatt tgtaatcaac ataaaatacg acattacaaa 720
tattgatata cgagaaaaga taaaaatgaa gaagtacgtt tcagaagaat aaaagtttaa 780
gagaagagga tgatttctta aatttcatta tcaaaaagat tcgaagataa aaattgaaaa 840
tttttattaa ctctcctcgg tgcttcttaa attttttgag atccgtttta gaattctaat 900
taattcttaa acatattttt atatgtaaca aaatatattt taatttttca atttaaaaac 960
ttcatcacta ctatatttaa acttaaactc cagatatatt tatgagaaat taaaattcat 1020
atttattaaa aaaatataca aaatatccaa attagatttc tcatttataa tgttcaaaag 1080
acgaaatgaa tctccattta gactccattc ttttaattga attcatgaaa atataaattt 1140
gtataaatac aatccacaat ctatttacat tttctctaat ctcctctatt atatccgcca 1200
attatcgaat aaaaacattg agacttgaga caagaagaaa ttgtgtaaaa atttgatgaa 1260
tatcgaacac aatcattttc catttggtaa ataaaattca aagcgatata ttcaataatc 1320
gttatcgtta cagaagtata tatatattat ttttaaatat cataaagatt atttagagtt 1380
tagtcgtaaa aaactcgaaa cgaaggacga gcttcgagtt tatgacgcga aatacaaata 1440
taaaactatc cattattcat gctaattggc gataatcgcg aagaattgta taatcggcga 1500
ttccttcgat tcactcgcgc gatgaaagtg caacgcgcaa ctgccacaac acacggggca 1560
aaaattgcca tggccaatgt ttaccatgga ccatgcaaga tgtgtaaata acgctaaaaa 1620
ttcttcacca agcgttagga aacaggccga gataacgagc ctctgcgtta cgcagcgaac 1680
gcgtaatatt ttaaagattt tacttacgaa cttttgaatt tttcgaaatt ttaaattttc 1740
cgccatttgt ttacgcttac attttgtatt acatataaga acagagaaag gaaaagattt 1800
cgtttttttt ttttaatttt tgaattcgtt cgcattttca aattttcaaa tttcctatct 1860
ttcgatcata ttggtttttt taattacgat tttatctttt tttaatcgaa aactttcgaa 1920
ttctttttat cctcgcctct tcgaatttca tatttcccgc ttttcgatcg cgttaataat 1980
ttttcgttta ctattttatc ttataatatt tcataattct acgattgtat ctaaagtcat 2040
tcggaatgat taaatatatt tcgttgaata atatatgcat ttaaagttta aaatttttta 2100
tatatctttc tttgtgtttt ttttattcat tcgtttatta tgtttcgggc agattgttta 2160
tcgaaacgaa cgatgagtca acattaaatt ccaatacgtt cctcgtaaaa aaaaaaaact 2220
tcaatacaac aacgtaaaaa ttgctcgtaa aattcataca cagatttcga aaaaaaaaat 2280
ataagatacg aaaaatttaa tttcatgaga acttgataag aatttttctt tttttctttt 2340
ccagaaattt ttattacaat attataatat gatattaatt aaaatttgtt ttgtaaatat 2400
tttaattata agttaattat aatctaaaaa aagaaaatca gcatgtattc aatttattat 2460
ttattaattt taattcaatt tgttaataaa atagttataa aagtttgaag tttgagagac 2520
cgtcaatcat tttatctgca acgatctaac gatctaagtc acgtagcaaa gcaaaagcaa 2580
aattctacgg aacaaaaaaa cgaaacgttt gcagcttgaa ttactgaaat cgagacgagc 2640
tccatgaacg ataattcaaa gaatcgtttg gtacaaaatg aaatttttta tgaaaacttt 2700
tttatctaaa attttgcatt attattatta cagatatcac agaaagatat aaatagatta 2760
tatcatttca ttagtgttat ttacataata aaatctatta aaagtttgtc aaactttttt 2820
ttttttaatt tttcattatc aacaaacgag gctaattatt gtagaaaaaa ttgtgagaat 2880
tatatgataa ctttaataac tgaaaataat agcacgcata tcgcataact gtcgtttata 2940
tgctggccta atttgccaaa gaataatttt tttcagagaa aaatttcgca aattgcttgt 3000
atttatttat ttttttttta atttttcttt agttttgtct attcttatta atattaattc 3060
tttaatattt cgtgatacac aggatattaa tttttttaaa aaatcttata ataatagatt 3120
tatgcacaga ttaaagaaat catttgtaag atctttccat aatacaataa aaatatatat 3180
tataattcaa aagaaaatta tatggcctcc aaaaatttga ttttctaaaa gtttcatttt 3240
cactttaaaa agaaaaaatt aatttttctt tcaaatatgt aaaataagtt acaataaaca 3300
tattgattaa tattttgata aataattaca atgaaattta tatgatttta aataaataca 3360
acctaaccta tttatctata ggacacattt gaaaaattat atataaccat taattaaata 3420
tatttttgta tgtaaaattc attttctgat agcattttaa taagatttca aatctatttt 3480
attaatgatt gacttgttag aaaaatattt taaatacctt actttttcta tgtttacgaa 3540
acactttatt ttgctgcgca ctttgtgcat acatattttc atggttcaca gtcgtgatcg 3600
cgactgttgg cctcctttta cttttcgttc gccgtgatca tccttttgct tttttctacg 3660
attttcgtgg tccattcacg aatgatcatt tctatttttc cctcttgtat ctctctatct 3720
tatttatcta aatcaggtca tccataaatt tctttctctt tgcttctatt ttttttttca 3780
tttatcattc gaagaaatca taatatataa ttaagaaaac aaaaatagag atgaatctga 3840
gaaaaaatag caaaaatatc gtcgccaatt tgttaaaatt ctggcaataa tcattaaaga 3900
aagattgtta aaatttattt ttaaaatata aaattcgtta aatgattcat taaattttat 3960
ataataaaac aacttaaatt aaaacttaaa ttttaaaaaa atgactaata ttatttgtaa 4020
ttatattagg attatataaa aataagaaaa atttaataat aactgaaagc aataaaattc 4080
taagtaataa atacacaata ataaaaaagt gatagaattt ctcatatttt atctaaaaaa 4140
attttcaaag ataaaattaa agatcaaaaa tttaatatca taaatgatga atcgaaattc 4200
aagaacgaca catggaaaat atttttctcc tttcttcgcg aattaaagaa aatgtaattc 4260
catttatttt tcttgtttct ttagtgaacg cggaaatcgg ttacgaaaga agcgacacgc 4320
gacaagatat cggtattcca agacgctgcg acgctgcacc tgtcttgatc gtgacgaatg 4380
ctgcacaaat aaaggaatgt cgtgataaag atctgaattg gaaattctga tgacgactta 4440
ctatcactac gtgatcctct ttattgtatg acatgttcga tacaatagac tttaaactag 4500
tatatgaaaa aacttacatt agaattcata acatacatta cattacatta gaatttttca 4560
ccattatatg tagatcagtc aataagtgct aactttttta ttctaaaaga ttttgcgatt 4620
ttgcattgaa gtttatttaa agaaattgaa caaaactgtg tatctaatag atcaattcaa 4680
atgtggtatc atgtaatggt tgtatattgt aacttttata ttttataaaa aaacaatgat 4740
atttgatcca tcgattatac tttgttcatg tatccatatt tatttcaaag attttaaagg 4800
tacagccttc atagatcaat gatgactcgt tatagattta atcagcacct aatctctata 4860
gaaactatta attagtcaca gaatgcatgt taatattttt gatgtaagag aaatttttct 4920
aacatcatta cagattctac aagattctaa cagattcttt ccatatttat ttcaaagatt 4980
ttaaatacgg ccttcataga tcagtgatga ctcgtcgtac atttaatcag cacataacct 5040
ctatagaaac tattaattag tcacagaatg catgttaata tttttgatct aaaagaaatt 5100
tttctaacat cattacagat tttacaagtt gacattcatt caaactgaaa aatgcatcaa 5160
tatatatatt aacaaaatta tgaatttaaa gaatggttaa aatggatata aagacaaaaa 5220
ttaattccac aaagctaaaa ctgatgacaa aaaaagaaaa tataatttct ataagttatg 5280
tttgtgtgtc aaataatggc aataaatatt ataactttaa aaaaaaacta ttacacatgc 5340
tgataattat taattacaac tgctaataat taaatttgtt tgtataatat catcaattaa 5400
tatataaaga tagctgcata acttttcttt tctctttcta gtttcattac catatctaaa 5460
ggtatttcat ataatgtcta tatttatgta taatattatt gatttctaag attaatttgt 5520
catgaaattt aatcactaat aagatccttg tatatcttaa catgagatca atttagatac 5580
atggaattat catttctcaa aggtttctag atgaaaagat tttatgcatt atccttctcc 5640
attatctaaa agagatattt agagaaacat aaaataaaat aatgaaaaaa aaatgaacat 5700
acaatttgta agtagctttt ttcactagct tttattttaa aaaaagattt ttatttatca 5760
ttagagtatg ttacatatac aatattgtat aataagttga tttatcaaat caatttcatt 5820
aatcaatctt atattacaat atactgaaca taatacatat aataaatgca tctttgagat 5880
tttcttgcct ttgaatttaa ttgagaatca aaaaatgctt tttaatatta ttgaatcaaa 5940
aataaaataa taatataata ataaataata aaataaaaaa gataaaaata ttcattaata 6000
atttcacagt atataataaa atttacgata aaagctttca aaattgcatt atgtttacta 6060
gttttgttgg aaattcataa catttaaaaa tattactgtg agatattaat tagatattaa 6120
tctttataaa tttatgaata gaaatctatt cgtatgtata ttggttgtat ctcgtgatag 6180
aaattttcat tatgtgaacg aatcataaat aaaaagctcg cgtgatattg gtatccattt 6240
catgaaaggg cagtgctcta atctaataag aaaaggaagt atttgctaag cccgcccttt 6300
gcttatatct aggtcaagaa taattgagta ggcgtcgcgt aaacacgtgt atcaagtggt 6360
tttctatttt ttttcagtca atcatcgcca tggttcgtct aaatttaata cggattctac 6420
ttattttatc aatgtcttga gctcgttcaa tgtgattaga ttcatatttt caaaatttaa 6480
atatttttta tatttgatat ttatatatta tttttcttgt tgtagaaaat attctcattt 6540
acattctatt taatatggat ataatatata tataattaat tttctaatta attgatacat 6600
atcaaattct tcattttcat atttctaatt atatatttat tatttgaatc gtgcaaatta 6660
gtactctaaa aaaaaaaatt ctcatttgtt tatataagag aaacaacttc aattaagatt 6720
ataatattat ataagatata ataattaata taaataaaat aatattaata ttaatacaat 6780
aatataataa agtaaatttc aataattcaa tgataaataa atacaatcaa gattaaaaaa 6840
tataaaataa tcctacatgt tataaaatag gtaattttta taaaaattac ctatttagat 6900
ataaatataa tatatagata taaatcttaa aatcactctt aactcaactt tggcaacata 6960
ctttctcaat taaatttcaa agcagcattt tgcatttcct aaatagtact tgaaagaaaa 7020
cattgtaagt aaaccatctt cgtatccaca agccttctcg tggaaaacaa cctctcgagg 7080
ccgagcgtga tctcttgcta gatgatgcaa cgtgtttttt tgcaacgaat actgcgaaca 7140
gactgacaaa gtcacttcgt gcatacatca gaacccgcaa ttttaatctt ctcttagact 7200
cgcaatcgat gaaacgcaat taaaactaat taaaaataaa aaaaaaatta aaggttaagt 7260
cgcgtttaga tattataacg ataagtgcaa tcgcgatttt tccacaattc tgaatggttc 7320
acctcatgcg accttatttc atatgtcatc gctcgttata gaacacaagt tgtgagcaaa 7380
tgttgttatt ctactaatga atactattaa tgttatttta atcaaaagat gataaaaatt 7440
cataaaataa aataataagt gcgtaattca ttaatagtgc caaagttata catattgaaa 7500
ttcttatgaa tataattaaa agaatgaaat tcaaatatta attttcattc tttattataa 7560
cttgtacttt attatatttt atttttttta ttttgctata tttagatttt tcacaaatac 7620
atgagcattc atagtatttt atatatatta tatatattag tgtactttat atataaattt 7680
aattttcaat ttatttattt attattgaaa tattacatta tatatattat atatatttta 7740
atttgtatat caatttattg cacattaata ttataaataa aatattgaaa taattctttt 7800
ccaagataca aaaagtatga aatttagata ggttaggatc cgtgaattat tattatacgt 7860
actttgatcg aatatagaca gtcaaatgtc atttaatagt taaataaatc acgaattcac 7920
gcgttctaaa aacaagatag aagtggaacg aaagcaaaac accgagttgc ggccaatgtc 7980
atccgacaag ttcttcatag acgacacgtt tcctttttac acgaggtgaa ccagagaaat 8040
ctcaggaaca agggatcgag atgcgtttcc ttatggggct cgtgagacct cgtcttagct 8100
tcgtattcac catgaccgtc agggtcgctc gtatcgatcg aatctcatgg cgatcttctt 8160
ttcctgtctc tcactttttt ttgtttcttt tttaagcgat atttaatttc atttcttaat 8220
ttatcaatta tcaattttct tatgatgatt atttttttcg aaggatatga aatatttatt 8280
attgaataat atttaataat attgatatta atattgatta aattttacga taataatttg 8340
ttgaaaggaa tattgtttaa tctaaattct caataatcat aaagttaaaa gtcaatttta 8400
aaataaatta ataattataa atataaaaat ttatttttaa cacaaattat ataatactta 8460
aagaaactgg aatccattaa tttaattaat taaaaaaatt tattaaatca atttaatcta 8520
attagtctat taatctattt gttattttct tcgattaaaa gattgaagat taaatttttt 8580
agtaatattt aaaaatatat taggaccaaa ttaatgtttt ttaaatataa aacgaaatca 8640
ttagtcctct ttcgaaattt ttgaaaaaaa taaactttaa attaataaaa taataaatgt 8700
aaataatata atttacataa taaaaattta aagatttaaa ttaatcaaga ttttcgcgat 8760
taatttattt tatcataaaa attgattaat ccattaaagt ctcaattgat aaaagaggtc 8820
ggtaatttat ttagcagggt tattcagtta agttgatgtg gtagtatggc gaccggatat 8880
cacaatctga aggaataaca aacgtgaaaa gaaaggtcag caatggatag accaaagagg 8940
cgaatcgaca aacaggcgaa aatagatgct gaccacctat gccaatgacc catattctct 9000
ctattctcgg cgtgccgcga attaaacatc gtcgttttaa tgccacatat tcaagattat 9060
gatcattgtt gccaatccgc tcgacttcga aaaaattaaa agaacaaaat tattggcaat 9120
agtatgaaaa atttaaatca ataaattaag aagttattaa tatataaaag attttaatac 9180
ctcactttcc aatagataaa atgaacaaaa aattaaattt attaaatcga actttatcat 9240
tttcaaaaaa agtataaaaa ctttattaac gattcgattt tatctaataa ttcaatatct 9300
taatttcata tcttcgtatc tttactttaa atttcataat tacattttaa tttaaagaaa 9360
aacaattgtt caaattctga atattgtatt tcttgtacat ttatatttca ctaaaataaa 9420
cgtatctttg atgatgcaac aaatcgaatg ttctcgtttg cataatagca acatttagaa 9480
atttttatgt cgtgctcgaa ctgaaataaa aatgagtaag gttaggtgta atgtagcgtc 9540
acctttgatg taatatcgcg tatgctaatg cgttgatcac tactatatat atatgggtat 9600
gaaaacatag ttgatgttat atgacgactc gaagaaagga aaaggaaacg tttcgtgaca 9660
tttcattcaa taaaaatgac ttaaacgatg aaattgaacc gttgagtcat aattttaata 9720
aaaaatataa agaaagaagt taaaaatatt tataatggat aatataataa actttaatgt 9780
ttttggaatt tttttttatt taagaataaa ttaatataaa aattttagaa aatctaacac 9840
ttattttcgt gatgcatgta taaaaagttt tcttgctaat cataaaaatg aaaaacatta 9900
aaaaattttg aaaaattcaa tattgaaatt ttacacaact cgtaagtaat aggtcataaa 9960
taaaatggaa ttcatgaaga gaaaacaaga aatgttattg tatcaggaat gagcaattgg 10020
ttgtcgatca attttaaaaa tacgcaagca tcacagattc taattctacc tgtgatctaa 10080
attctattag aatacctgtt tgatatttga aagaagaaag aacagatcga taaaataatt 10140
aatcttataa tacaaatttg agacacttgt tgaaggttat aaaaattaaa aatttttaat 10200
tttaattaaa aataatctta caaatcttac aaatgctttt tcttcaatta taatttagct 10260
ttccttaatt ttcttttttt ttttattttt atttcattaa ataagcaatt cttaaatttt 10320
tttaacacat ttaattgaat atattatatt cagttaaaaa tataaatata tatatatata 10380
tatatatata tatataaaaa taaatttttc atatcaaatt tatcgaaaat attaaaattt 10440
gttaatcttt atttttatga tatatttttt gcaaaagatt ataaaaataa gagaaagatt 10500
aaaaacatct taattttttt ttaaaacatc atttaatatt atttttcttt tttcagaatg 10560
caactcacga cgtgttgcaa atgttattcg ttaaaaacgg ggacattgtt cactggaata 10620
ttgggcatag taagtatatg ataaaaaaat taattagttt tttattacat ttaatcaaaa 10680
aaaaaaaaaa aaaatttttt atagaaacat atttattcga ttcttattag gttttatcaa 10740
ttatctcatt gatcttaatc tttactttaa atgtcgaatg gaaaacaata ttgatcgaca 10800
ttgtagacca gagcatcgtc aggatcattt ttgcgataaa tttatgcatg acgattctga 10860
tttcaacatt gcttatagtt ggcgctctta aggtaaaatc taaatcaatt tcatgtttat 10920
aaaataaaat tttttattat aaatattttt cgaatttgtt tgatttattg gttatttaaa 10980
tttatataaa ttaaaacatt tcttttttag aaaaaaacat ttttaatgct accatgggta 11040
gttttgggtt taatcttagc agttggttta ttggtgagtg tcctgtatac gtccatcatg 11100
ttcttcatat atcatgacgt aataattgga attctgtggc tcatcattgg ccttctaacc 11160
attggtgagt agcgtatagt tacttttata ttttgtattt tatattttca tttattttgt 11220
tttcttgtga atttttattt gaatatcttc ttagaaatta ttatttataa attaaaaaca 11280
tttaaagtac tttaaaaaat acaatcgaat cttgatattt cgaatcgcaa aagaggttat 11340
gggaatttta tcttcgaact ttcctaagaa tgagttattc atacgacagc cgtgataaaa 11400
atttgacttg tagaattgta gagaaaggac tacttcgact tcaaatttca aatcaaatat 11460
agtaaaattc gagttatata aatttatatc ttctattgta taagttttta tagaaatgaa 11520
cacgaataag caaaattgta atattttaga gaatttctag ttacagattt aatatttaaa 11580
atataaaaaa aaaaattttg ttatattaat aatcttcaat tttttaaaca caataagttg 11640
atcattttaa aaaatgtttt aaatataaat tggtttataa ataatcttaa tctttttttt 11700
ttttttcagt gatctatgta tatttgtggt tggtagtata tagttatttc caacaattaa 11760
ggtatgataa gatgagcagc cgaatcgatc catatggaag accttataat tatcgaagac 11820
cttaaaaaca aatcgataat ttcacaaaga agaaaatgat cgagatgttt tctcttcgca 11880
aaataattag actatttttt tcttttaaat aaaaaatcaa aaaaaaaaaa taaattatat 11940
gaaagaatag tctaattact ttgagagaat ttatatactt aatgatcaat tagcacaatc 12000
gcaccaaagc atgagagaca acgcctttcg tcgataaaga atttatttat cgaaaactgc 12060
agaatcatat ttgtaaaata agattaatag tcatgagtag attagaatat atttatggca 12120
ccaacatgct ccctcgaatg atattcgata ttttttatat atttattttt aacaattttt 12180
tttgtataaa aacttgaaac tttatatcac atatatatat ttcgtatttt taaaataaaa 12240
attaaattat taaacataag aaatatataa tacataatca aaatgaatca ttcatacatc 12300
aaaaaatatc aaaaaatttt tattcatata gatgagatta taaataagat aaaactttag 12360
ttatattttc actgctggaa acagaatttt aacctcgttt catgtttacc aacctttaac 12420
ctaataattg taaagaatgt cagcgaaaga cgaggagaga ttgagaagta ttaaagaaat 12480
ttattgtgaa ctattgtatc gctgtggaat tattcgcttc actgggttca aaggagactc 12540
tcgatcatat atccgcgata acagcatatc acgaataaat aatataatag tgaatcgaaa 12600
aacgagcatc gttcgagggt gtaaaaacac aattgtgaaa cgcgttttct ttttttcttc 12660
gagaaccgac actattgtcg gttcgttatt gcgatatcaa aaagacgagg ctgatcccta 12720
gcgggatgaa aatagattcc attttcaagg ataacgaaaa tattaagcgt atgtaaaaat 12780
atacgcgttg ccacattgca gtgaaccagt ctcctacgca aggttaacga ctgactttat 12840
ttatttttcc tgctcctccc cacttttatt tctcttctat tctttctata ccgcctataa 12900
tataccattt gtaattattg ttacaaactg tgagaaataa attattatta tttgtttgta 12960
<210> 5
<211> 370
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 5
Met Cys Met Glu Met Leu Arg Lys Phe Ile Trp Ala Ile Leu Leu Phe
1 5 10 15
Cys Asp Phe Pro Pro Trp Ser Cys Gly Asn Phe Ala Ser Lys Asn Phe
20 25 30
Glu Glu Lys Leu Val Lys Leu Ser Lys Val Ile Glu Tyr Ile His Gln
35 40 45
Arg Pro Gln Gln Met Asn Ile Asp Val Thr Leu Ser Leu Thr Ile Val
50 55 60
Gln Gly Lys Thr Ile Lys Ile Leu Gln Ile Tyr Asn Phe Asn Lys His
65 70 75 80
Arg Tyr Phe Pro Asn Ile Glu Tyr Phe Thr Ser Met Leu Phe Phe Thr
85 90 95
Ala Asn Ile Ala Ala Ile Phe Leu His Lys Asn Ala Arg Phe Leu Ala
100 105 110
Asp Lys His Arg Asp Ile Leu Val Thr Ile Leu Lys Leu Cys Asp Leu
115 120 125
Thr Arg Arg Asp Leu Leu Asn Lys Ile Val Leu Glu Asn Glu Asp Ile
130 135 140
Arg Leu Leu His Glu Thr Ile Asn Tyr Pro Asn Leu Trp Met Lys Lys
145 150 155 160
Ile Ser Trp Arg His Gly Ala Leu Ala Lys Gly Arg Ala Asn Ile Gly
165 170 175
Leu Pro Tyr Arg Asp Ile Arg Asp Leu Val Met Gln Gly Val Pro Asn
180 185 190
Glu Glu Glu Ser Asp Arg Cys Leu Ala Glu Ile Val Arg Asn Lys Phe
195 200 205
Asn Ser Asp His Arg Ile Pro Gly Leu Cys Leu Glu Ile Leu Thr Thr
210 215 220
Arg Glu Ser Ser Lys Gly Tyr Pro Leu Thr His Arg Leu Leu Ile Val
225 230 235 240
Gln Ile Ala Lys Ile Met Glu Cys Asp Gln Gly Leu Pro Ser Ser Glu
245 250 255
Leu Ile Leu Phe Tyr Cys Ser Ala Ile Phe Gln Asp Leu Ile Asp Ile
260 265 270
Glu Ile Ala Gly Phe Pro Tyr Gln Thr Pro Asp Leu Met Met Glu Gln
275 280 285
Val Val Leu Cys Gly Met Glu Gly Phe Leu Glu Phe Thr Asp Lys His
290 295 300
Tyr Gln Arg Leu Ile Leu Asp Trp Ser His Pro Ser Gly Cys Phe Ser
305 310 315 320
Ser Phe Gly Asn Lys Phe Leu Asn Asn Lys Met Arg Val Ile Arg Arg
325 330 335
Thr Ser Met Gln Thr Asp Phe Gly Cys Asp Asn His Ala Thr Gly Leu
340 345 350
Ala Ala Ala Ser Leu Ser Leu Phe Ile Arg Lys Ser Val Glu Asn Thr
355 360 365
Tyr Glu
370
<210> 6
<211> 339
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 6
Met Cys Met Glu Met Leu Arg Lys Phe Ile Trp Ala Ile Leu Leu Phe
1 5 10 15
Cys Asp Phe Pro Pro Trp Ser Cys Gly Asn Phe Ala Ser Lys Asn Phe
20 25 30
Glu Glu Lys Leu Val Lys Leu Ser Lys Val Ile Glu Tyr Ile His Gln
35 40 45
Arg Pro Gln Gln Met Asn Ile Asp Val Thr Leu Ser Leu Thr Ile Val
50 55 60
Gln Ala Asn Ile Ala Ala Ile Phe Leu His Lys Asn Ala Arg Phe Leu
65 70 75 80
Ala Asp Lys His Arg Asp Ile Leu Val Thr Ile Leu Lys Leu Cys Asp
85 90 95
Leu Thr Arg Arg Asp Leu Leu Asn Lys Ile Val Leu Glu Asn Glu Asp
100 105 110
Ile Arg Leu Leu His Glu Thr Ile Asn Tyr Pro Asn Leu Trp Met Lys
115 120 125
Lys Ile Ser Trp Arg His Gly Ala Leu Ala Lys Gly Arg Ala Asn Ile
130 135 140
Gly Leu Pro Tyr Arg Asp Ile Arg Asp Leu Val Met Gln Gly Val Pro
145 150 155 160
Asn Glu Glu Glu Ser Asp Arg Cys Leu Ala Glu Ile Val Arg Asn Lys
165 170 175
Phe Asn Ser Asp His Arg Ile Pro Gly Leu Cys Leu Glu Ile Leu Thr
180 185 190
Thr Arg Glu Ser Ser Lys Gly Tyr Pro Leu Thr His Arg Leu Leu Ile
195 200 205
Val Gln Ile Ala Lys Ile Met Glu Cys Asp Gln Gly Leu Pro Ser Ser
210 215 220
Glu Leu Ile Leu Phe Tyr Cys Ser Ala Ile Phe Gln Asp Leu Ile Asp
225 230 235 240
Ile Glu Ile Ala Gly Phe Pro Tyr Gln Thr Pro Asp Leu Met Met Glu
245 250 255
Gln Val Val Leu Cys Gly Met Glu Gly Phe Leu Glu Phe Thr Asp Lys
260 265 270
His Tyr Gln Arg Leu Ile Leu Asp Trp Ser His Pro Ser Gly Cys Phe
275 280 285
Ser Ser Phe Gly Asn Lys Phe Leu Asn Asn Lys Met Arg Val Ile Arg
290 295 300
Arg Thr Ser Met Gln Thr Asp Phe Gly Cys Asp Asn His Ala Thr Gly
305 310 315 320
Leu Ala Ala Ala Ser Leu Ser Leu Phe Ile Arg Lys Ser Val Glu Asn
325 330 335
Thr Tyr Glu
<210> 7
<211> 2891
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 7
attaaaatct catggactta tgggggtttg cggtggggtt gtccctataa aatccttgga 60
aaatcgagat cccgtttctt cacagctgtg aacgaatgtg tatggaaatg cttagaaagt 120
tcatttgggc gattctgctt ttctgcgatt ttccaccatg gagctgtgga aactttgctt 180
ccaagaattt tggtaagatt ggaaaaatct tgaaatcttt cctaatttat ttttatttat 240
tatctttttt aacaattaaa atgatacaaa aaattattcg aaaaatcaaa atatgaatta 300
taaatttgaa tttgacagat tagatttaga atttaaaatt ttatttattt atcatattat 360
ttatcaataa cagatttaat aaaatggaaa ctcttgaaat ttttgatatt ttaaaaaaaa 420
aatataagat ttaattaatt ttatctgttg taaaatcaaa ataggaataa taaataaaat 480
aatttctatt cttggaaatg tcaagaacat ttttctttgt acatttattt tttttttcaa 540
atgataaaaa ttattctaat tcattgttaa aaatgcaaaa catttataat aatttactat 600
ttataataaa ttatgtaaat ttaaattatt tataattaat atattaatta atatttataa 660
tacatttata attatattta tatcaaatat cctaaatgtt tctatatatt tattaaaaat 720
tagataaaaa aattgtataa ttcagtgacc tgtttaaatt tgttgttttt ttaatcatta 780
aatccgtcac tgtttattac agaagaaaaa ttagtcaagt tgtctaaagt gatcgaatac 840
attcatcaaa gacctcaaca aatgaatatc gacgttacgt tatctttaac tattgtccaa 900
ggtaaaacaa taaaaatatt gcaaatttac aattttaaca agcatcgtta ctttccaaac 960
attgaatatt tcacttcaat gttatttttt acagctaaca ttgctgctat ttttttacac 1020
aaaaatgcac gatttttagc cgataaacat cgagatatac ttgtaacgat tttaaagctt 1080
tgtgatttaa cgagacggga tttattgaat aaaatcgttt tagagaacga agatatccga 1140
ttgtgtaaga aaatttaatt ttaacgaaag aaaaaaaaat ttattttcga atacaataat 1200
gaaaacatag tcaattcata gtgcacgaaa cgataaatta tcccaattta tggatgaaaa 1260
agatatcatg gcggcatggc gctctagcaa aagggagagc caatatcgga ttaccttatc 1320
gagatatacg agatctggta atgcaaggag tgcctaacga ggaagaaagc gatcgatgcc 1380
tcgctgaaat cgttcgaaat aagttcaatt cggatcatag aattcctggt ttatgcctgg 1440
aaatattaac tactcgagaa tcaagcaaag gatatcctct cactcatcga ttgttaatcg 1500
ttcaaattgc caaaatagtt agtatatcat cacaaaattt ttgttcttcc acacgtagga 1560
ttataagtat ccaaaaattt gaaaaattat tcttcaaacg aaaataaaaa ttatatttct 1620
ggttttatta tgaataataa aaaaaaattt ttatttttat tttatttttg cttaaagaat 1680
tattcttttt gacgacactg tgtatatctt taattaaacg taatttcttt tcagatggaa 1740
tgtgaccaag gtcttccatc ttcagaatta atactttttt attgttctgc aatttttcaa 1800
gatctgatcg atatcgagat agctggattt ccttatcaaa caccagattt aatgatggaa 1860
cagggtaaag aatttaaaaa atacttcgaa attttttata attttgtata attattttac 1920
acaatttctt ttctttcgtg cgtttctttg aaagttgttt tatgcggcat ggaaggtttc 1980
cttgaattca ctgataagca ttatcaacga ttaatattgg actggtcaca tcctagcggt 2040
tgcttcagtt ctttcgggta cgtatctgat agattacact gtttttctcg catctcacct 2100
ttgactttgc ttacaatcga tcacaaaaat atgtaatatt ctttttcaca gaaataaatt 2160
tcttaataac aaaatgcgtg tgatacgaag aacttcgatg caaactgatt ttggctgcga 2220
taatcatgct actggtttag ccgctgcttc tctttctttg tttattcgca aaagtgtaga 2280
gaatacgtac gaatgaacaa cacaatcgtt tgcacacata tgtactgact atatatatat 2340
agatacaata taatatatat agaataaaat ctaaagtcgc gttttaagag aaaaataata 2400
aaattcttgt agatatttta tattgcttaa aaaacatttc gaatattgcg aatgtttcgc 2460
gaaatatctc acgaaatatc gaacattttc tataaataat actgtcaata aaaagtttct 2520
tgttatcact tgaaaacgcg gctgaacgaa aaaatgaaaa gaatgcgaaa gaagaagaaa 2580
ataataaaat gaaactacaa acaaataata ataatttatt tctcacagtt tgtaacaata 2640
attacaaatg gtatattata ggcggtatag aaagaataga agagaaataa aagtggggag 2700
gagcaggaaa aataaataaa gtcagtcgtt aaccttgcgt aggagactgg ttcactgcaa 2760
tgtggcaacg cgtatatttt tacatacgct taatattttc gttatccttg aaaatggaat 2820
ctattttcat cccgctaggg atcagcctcg tctttttgat atcgcaataa cgaaccgaca 2880
atagtgtcgg t 2891
Claims (10)
1.一种鉴定蜜蜂分化关键基因的方法，其特征在于，包括：
获得多个蜜蜂96h幼虫的全基因组信息，通过三维基因组技术进行分析得到多个蜜蜂基因拓扑相关结构域，根据蜂王浆主蛋白家族成员在所述多个蜜蜂基因拓扑相关结构域的分布确定和蜜蜂分化相关的关键基因。
2.根据权利要求1所述的方法，其特征在于，所述蜂王浆主蛋白家族成员包括：MRJP1、MRJP2、MRJP3、MRJP4、MRJP5、MRJP6、MRJP7、MRJP8和MRJP9。
3.根据权利要求1或2所述的方法，其特征在于，所述获得多个蜜蜂96h幼虫的全基因组信息为：
获得多个蜜蜂96h幼虫的组织，经过预处理、酶切、生物素标记、平末端连接及DNA纯化提取后得到Hi-C样本，使用所述Hi-C样本建立蜜蜂全基因信息文库并测序用于后续的三维基因组技术分析。
4.根据权利要求1或2所述方法，其特征在于，所述通过三维基因组技术进行分析得到多个蜜蜂基因拓扑相关结构域为使用80～100kb的分辨率对染色体进行bin的划分，后通过Insulation算法或directionality index算法鉴定TAD的边界得到多个蜜蜂基因拓扑相关结构域。
5.根据权利要求1或2所述的方法，其特征在于，所述确定和蜜蜂分化相关的关键基因为：
统计蜂王浆主蛋白家族成员在所有拓扑相关结构域中的分布，确定所述蜂王浆主蛋白家族成员最多的拓扑相关结构域中的基因为和蜜蜂分化相关的关键基因。
6.权利要求1-5任一项所述方法在辅助蜜蜂级型分化中的应用。
7.L-木酮糖还原酶、LOC724335蛋白或LOC724286蛋白在鉴定分化为蜂王或者工蜂的蜜蜂幼虫中的应用，其特征在于，所述L-木酮糖还原酶含有如SEQ ID NO:1所示的氨基酸序列，所述LOC724286蛋白含有如SEQ ID NO:3所示的氨基酸序列，所述LOC724335蛋白含有如SEQ ID NO:5和SEQ ID NO:6所示的氨基酸序列。
8.根据权利要求7所述的应用，其特征在于，所述应用为：
对蜜蜂幼虫的L-木酮糖还原酶进行检测，L-木酮糖还原酶表达量高的分化为蜂王，L-木酮糖还原酶表达量低的分化为工蜂；和/或，
对蜜蜂幼虫的LOC724286蛋白进行检测，LOC724286蛋白表达量高的分化为蜂王，LOC724335蛋白表达量低的分化为工蜂；和/或，
对蜜蜂幼虫的LOC724335蛋白进行检测，LOC724335蛋白表达量高的分化为工蜂，表达量低的分化为蜂王。
9.L-木酮糖还原酶、LOC724286蛋白或LOC724335蛋白在调控蜜蜂分化中的应用，其特征在于，所述L-木酮糖还原酶含有如SEQ ID NO:1所示的氨基酸序列，所述LOC724286蛋白含有如SEQ ID NO:3所示的氨基酸序列，所述LOC724335蛋白含有如SEQ ID NO:5和SEQ IDNO:6所示的氨基酸序列。
10.根据权利要求7或9所述的应用，其特征在于，编码所述L-木酮糖还原酶的基因包括如SEQ ID NO:2所示的核苷酸序列，和/或，编码所述LOC724286蛋白的基因包括如SEQ IDNO:4所示的核苷酸序列，和/或，编码所述LOC724335蛋白的基因包括如SEQ ID NO:7所示的核苷酸序列。
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