CN112243462B - 产生核酸文库的方法以及用于实践所述方法的组合物和试剂盒 - Google Patents

产生核酸文库的方法以及用于实践所述方法的组合物和试剂盒 Download PDF

Info

Publication number
CN112243462B
CN112243462B CN201980038046.3A CN201980038046A CN112243462B CN 112243462 B CN112243462 B CN 112243462B CN 201980038046 A CN201980038046 A CN 201980038046A CN 112243462 B CN112243462 B CN 112243462B
Authority
CN
China
Prior art keywords
ssna
ssb
oligonucleotide
bound
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201980038046.3A
Other languages
English (en)
Chinese (zh)
Other versions
CN112243462A (zh
Inventor
R·格林
J·卡普
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of California San Diego UCSD
Original Assignee
University of California San Diego UCSD
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of California San Diego UCSD filed Critical University of California San Diego UCSD
Publication of CN112243462A publication Critical patent/CN112243462A/zh
Application granted granted Critical
Publication of CN112243462B publication Critical patent/CN112243462B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2533/00—Reactions characterised by the enzymatic reaction principle used
    • C12Q2533/10—Reactions characterised by the enzymatic reaction principle used the purpose being to increase the length of an oligonucleotide strand
    • C12Q2533/107—Probe or oligonucleotide ligation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201980038046.3A 2018-06-06 2019-06-05 产生核酸文库的方法以及用于实践所述方法的组合物和试剂盒 Active CN112243462B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862681524P 2018-06-06 2018-06-06
US62/681,524 2018-06-06
PCT/US2019/035617 WO2019236726A1 (en) 2018-06-06 2019-06-05 Methods of producing nucleic acid libraries and compositions and kits for practicing same

Publications (2)

Publication Number Publication Date
CN112243462A CN112243462A (zh) 2021-01-19
CN112243462B true CN112243462B (zh) 2025-02-25

Family

ID=67211813

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201980038046.3A Active CN112243462B (zh) 2018-06-06 2019-06-05 产生核酸文库的方法以及用于实践所述方法的组合物和试剂盒

Country Status (8)

Country Link
US (2) US11629345B2 (enExample)
EP (1) EP3802864A1 (enExample)
JP (2) JP7537748B2 (enExample)
KR (1) KR102767028B1 (enExample)
CN (1) CN112243462B (enExample)
AU (1) AU2019280712B2 (enExample)
CA (1) CA3100983A1 (enExample)
WO (1) WO2019236726A1 (enExample)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019236726A1 (en) 2018-06-06 2019-12-12 The Regents Of The University Of California Methods of producing nucleic acid libraries and compositions and kits for practicing same
WO2020206143A1 (en) * 2019-04-05 2020-10-08 Claret Bioscience, Llc Methods and compositions for analyzing nucleic acid
AU2020363787A1 (en) 2019-10-09 2022-04-21 Claret Bioscience, Llc Methods and compositions for analyzing nucleic acid
WO2022076574A1 (en) 2020-10-08 2022-04-14 Claret Bioscience, Llc Methods and compositions for analyzing nucleic acid
GB2606158B (en) * 2021-04-26 2023-12-27 Wobble Genomics Ltd Amplification of single stranded DNA
IL310293A (en) * 2021-07-22 2024-03-01 Mgi Tech Co Ltd STLFR cut-tie
EP4457356A4 (en) * 2021-12-24 2025-10-29 Pacbridge Partners Ii Invest Co Ltd COMPOSITIONS AND METHODS FOR IDENTIFYING GENE FUSIONS

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103354840A (zh) * 2011-01-31 2013-10-16 欧凌科公司 核酸外切酶可实现的邻近延伸分析
WO2016058517A1 (en) * 2014-10-14 2016-04-21 Bgi Shenzhen Co., Limited Mate pair library construction
WO2016156529A1 (en) * 2015-03-31 2016-10-06 Qiagen Gmbh Efficiency improving ligation methods

Family Cites Families (104)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989001526A1 (en) 1987-08-07 1989-02-23 Genelabs Incorporated Coincidence cloning method and library
US6261774B1 (en) 1990-06-11 2001-07-17 Gilead Sciences, Inc. Truncation selex method
US5744306A (en) 1993-04-19 1998-04-28 Emory University Methods for nucleic acid detection, sequencing, and cloning using exonuclease
US5871697A (en) 1995-10-24 1999-02-16 Curagen Corporation Method and apparatus for identifying, classifying, or quantifying DNA sequences in a sample without sequencing
ATE346165T1 (de) 1995-11-29 2006-12-15 Anthony Nolan Bone Marrow Trus Verfahren zur trennung und/oder erkennung von dns-molekülen
US5861246A (en) 1996-01-24 1999-01-19 Yale University Multiple selection process for binding sites of DNA-binding proteins
AU2324997A (en) 1996-03-15 1997-10-01 Penn State Research Foundation, The Detection of extracellular tumor-associated nucleic acid in blood plasma or ser um using nucleic acid amplification assays
PL331513A1 (en) 1996-06-06 1999-07-19 Lynx Therapeutics Method of sequencing, by a ligand effect, specific encoded adapters and composition containing double-string oligonucleotidic adapters
US6083693A (en) 1996-06-14 2000-07-04 Curagen Corporation Identification and comparison of protein-protein interactions that occur in populations
US6013438A (en) 1996-11-27 2000-01-11 Baylor College Of Medicine Assay for detecting apoptotic cells
WO1999002725A1 (en) 1997-07-11 1999-01-21 Brax Group Limited Categorising nucleic acid
US6607878B2 (en) 1997-10-06 2003-08-19 Stratagene Collections of uniquely tagged molecules
NO986133D0 (no) 1998-12-23 1998-12-23 Preben Lexow FremgangsmÕte for DNA-sekvensering
WO2000039333A1 (en) 1998-12-23 2000-07-06 Jones Elizabeth Louise Sequencing method using magnifying tags
JP2002540802A (ja) 1999-04-06 2002-12-03 イェール ユニバーシティ 配列標識の固定されたアドレス分析
WO2001012855A2 (en) 1999-08-13 2001-02-22 Yale University Binary encoded sequence tags
WO2001049882A2 (en) 1999-12-29 2001-07-12 Keygene N.V. METHOD FOR GENERATING OLIGONUCLEOTIDES, IN PARTICULAR FOR THE DETECTION OF AMPLIFIED RESTRICTION FRAGMENTS OBTAINED USING AFLP$m(3)
US7300751B2 (en) 2000-06-30 2007-11-27 Syngenta Participations Ag Method for identification of genetic markers
US6596490B2 (en) 2000-07-14 2003-07-22 Applied Gene Technologies, Inc. Nucleic acid hairpin probes and uses thereof
JP4340779B2 (ja) 2000-10-05 2009-10-07 独立行政法人理化学研究所 可変突出部位を含むオリゴヌクレオチドリンカー及び前記リンカーを用いたポリヌクレオチドライブラリーの調製方法
MXPA02012739A (es) 2001-03-09 2004-04-20 Nugen Technologies Inc Metodos y composiciones para amplificacion de secuencias de arn.
US9261460B2 (en) 2002-03-12 2016-02-16 Enzo Life Sciences, Inc. Real-time nucleic acid detection processes and compositions
US20040006033A1 (en) 2001-08-06 2004-01-08 Zhu York Yuan-Yuan Methods for identifying low-abundance polynucleotides and related compositions
US6927028B2 (en) 2001-08-31 2005-08-09 Chinese University Of Hong Kong Non-invasive methods for detecting non-host DNA in a host using epigenetic differences between the host and non-host DNA
US20030219878A1 (en) 2002-03-13 2003-11-27 Large Scale Biology Corporation Sticky rice
EP2348124B1 (en) 2002-10-30 2013-12-11 Nuevolution A/S Synthesis of a bifunctional complex
US8114978B2 (en) 2003-08-05 2012-02-14 Affymetrix, Inc. Methods for genotyping selected polymorphism
ATE435301T1 (de) 2003-10-16 2009-07-15 Sequenom Inc Nicht invasiver nachweis fötaler genetischer merkmale
US8192937B2 (en) 2004-04-07 2012-06-05 Exiqon A/S Methods for quantification of microRNAs and small interfering RNAs
JP5149013B2 (ja) 2004-11-29 2013-02-20 セクエノム，インコーポレイティド メチル化ｄｎａを検出する手段、及び方法
US7169561B2 (en) 2004-12-29 2007-01-30 Applera Corporation Methods, compositions, and kits for forming self-complementary polynucleotides
WO2007018601A1 (en) 2005-08-02 2007-02-15 Rubicon Genomics, Inc. Compositions and methods for processing and amplification of dna, including using multiple enzymes in a single reaction
MY143596A (en) 2005-08-11 2011-06-15 Synthetic Genomics Inc In vitro recombination method
WO2007024653A2 (en) 2005-08-19 2007-03-01 Bioventures, Inc. Method and substances for isolating micro rnas
WO2007037678A2 (en) 2005-09-29 2007-04-05 Keygene N.V. High throughput screening of mutagenized populations
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
GB0524069D0 (en) 2005-11-25 2006-01-04 Solexa Ltd Preparation of templates for solid phase amplification
EP3617321B1 (en) 2006-05-31 2024-10-23 Sequenom, Inc. Kit for the extraction and amplification of nucleic acid from a sample
JP2009540802A (ja) 2006-06-16 2009-11-26 セクエノム， インコーポレイテッド サンプルからの核酸を増幅、検出および定量するための方法および組成物
US8932994B2 (en) 2006-08-24 2015-01-13 Illumina, Inc. Method for retaining even coverage of short insert libraries
US20100311602A1 (en) 2006-10-13 2010-12-09 J. Craig Venter Institute, Inc. Sequencing method
WO2008093098A2 (en) 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
WO2009032781A2 (en) 2007-08-29 2009-03-12 Sequenom, Inc. Methods and compositions for universal size-specific polymerase chain reaction
WO2009032779A2 (en) 2007-08-29 2009-03-12 Sequenom, Inc. Methods and compositions for the size-specific seperation of nucleic acid from a sample
AU2009205523A1 (en) 2008-01-14 2009-07-23 Applied Biosystems, Llc Compositions, methods, and kits for detecting ribonucleic acid
US8034568B2 (en) 2008-02-12 2011-10-11 Nugen Technologies, Inc. Isothermal nucleic acid amplification methods and compositions
CA2718905A1 (en) 2008-03-17 2009-09-24 Expressive Research B.V. Expression-linked gene discovery
WO2009120808A2 (en) 2008-03-26 2009-10-01 Sequenom, Inc. Restriction endonuclease enhanced polymorphic sequence detection
US9115352B2 (en) 2008-03-31 2015-08-25 Sloning Biotechnology Gmbh Method for the preparation of a nucleic acid library
US8029993B2 (en) 2008-04-30 2011-10-04 Population Genetics Technologies Ltd. Asymmetric adapter library construction
US8476013B2 (en) 2008-09-16 2013-07-02 Sequenom, Inc. Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
EP3103871B1 (en) 2008-09-16 2020-07-29 Sequenom, Inc. Processes for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for fetal nucleic acid quantification
EP3514244B1 (en) 2009-04-03 2021-07-07 Sequenom, Inc. Nucleic acid preparation methods
US9631227B2 (en) 2009-07-06 2017-04-25 Trilink Biotechnologies, Inc. Chemically modified ligase cofactors, donors and acceptors
GB0912909D0 (en) 2009-07-23 2009-08-26 Olink Genomics Ab Probes for specific analysis of nucleic acids
WO2011143659A2 (en) 2010-05-14 2011-11-17 Fluidigm Corporation Nucleic acid isolation methods
US8828688B2 (en) 2010-05-27 2014-09-09 Affymetrix, Inc. Multiplex amplification methods
EP2580378A4 (en) 2010-06-08 2014-01-01 Nugen Technologies Inc MULTIPLEX SEQUENCING METHODS AND COMPOSITION
US8575071B2 (en) 2010-11-03 2013-11-05 Illumina, Inc. Reducing adapter dimer formation
WO2012103154A1 (en) 2011-01-24 2012-08-02 Nugen Technologies, Inc. Stem-loop composite rna-dna adaptor-primers: compositions and methods for library generation, amplification and other downstream manipulations
SG10201605049QA (en) 2011-05-20 2016-07-28 Fluidigm Corp Nucleic acid encoding reactions
WO2013019361A1 (en) 2011-07-07 2013-02-07 Life Technologies Corporation Sequencing methods
JP6430253B2 (ja) 2011-11-05 2018-11-28 プレジデント アンド フェローズ オブ ハーバード カレッジ 相互作用を検出および測定するための核酸ベースのリンカー
WO2013102091A1 (en) 2011-12-28 2013-07-04 Ibis Biosciences, Inc. Nucleic acid ligation systems and methods
US10227587B2 (en) 2012-01-10 2019-03-12 Berry Genomics Co., Ltd. Method for constructing a plasma DNA sequencing library
EP2802666B1 (en) 2012-01-13 2018-09-19 Data2Bio Genotyping by next-generation sequencing
EP2820129A1 (en) 2012-03-02 2015-01-07 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
KR101922124B1 (ko) 2012-08-27 2018-11-26 삼성전자주식회사 시료 중 ｒｎａ로부터 ｄｎａ를 증폭하는 방법
CN104781421B (zh) 2012-09-04 2020-06-05 夸登特健康公司 检测稀有突变和拷贝数变异的系统和方法
DK2914741T3 (da) 2012-11-02 2017-11-20 Life Technologies Corp Hidtil ukendte sammensætninger og fremgangsmåder til forbedring af PCR-specificitet
WO2014110272A1 (en) 2013-01-09 2014-07-17 The Penn State Research Foundation Low sequence bias single-stranded dna ligation
US9982255B2 (en) 2013-03-11 2018-05-29 Kailos Genetics, Inc. Capture methodologies for circulating cell free DNA
RU2694686C2 (ru) 2013-03-12 2019-07-16 Е.И.Дюпон Де Немур Энд Компани Способы идентификации вариантных сайтов распознавания для редкощепящих сконструированных средств для индукции двунитевого разрыва, и композиции с ними, и их применения
US9255265B2 (en) 2013-03-15 2016-02-09 Illumina, Inc. Methods for producing stranded cDNA libraries
WO2014145078A1 (en) 2013-03-15 2014-09-18 Verinata Health, Inc. Generating cell-free dna libraries directly from blood
WO2014151117A1 (en) 2013-03-15 2014-09-25 The Board Of Trustees Of The Leland Stanford Junior University Identification and use of circulating nucleic acid tumor markers
AU2014302277A1 (en) 2013-06-27 2015-12-24 10X Genomics, Inc. Compositions and methods for sample processing
US9765375B2 (en) 2013-06-28 2017-09-19 General Electric Company Methods for developing binding-elements and uses thereof
ES2764096T3 (es) 2013-08-19 2020-06-02 Abbott Molecular Inc Bibliotecas de secuenciación de próxima generación
WO2015073711A1 (en) 2013-11-13 2015-05-21 Nugen Technologies, Inc. Compositions and methods for identification of a duplicate sequencing read
WO2015094861A1 (en) 2013-12-17 2015-06-25 Clontech Laboratories, Inc. Methods for adding adapters to nucleic acids and compositions for practicing the same
EP3363904B1 (en) 2014-01-31 2019-10-23 Swift Biosciences, Inc. Improved methods for processing dna substrates
CA2931140C (en) 2014-02-05 2022-08-30 Fraunhofer-Gesellschaft Zur Forderung Der Angewandten Forschung E.V. Error-free sequencing of dna
EP3114231B1 (en) 2014-03-03 2019-01-02 Swift Biosciences, Inc. Enhanced adaptor ligation
EP2921556A1 (en) * 2014-03-21 2015-09-23 Lexogen GmbH Copy number preserving RNA analysis method
EP4358097A1 (en) 2014-07-25 2024-04-24 University of Washington Methods of determining tissues and/or cell types giving rise to cell-free dna, and methods of identifying a disease or disorder using same
DK3192869T3 (da) 2014-09-12 2019-05-20 Mgi Tech Co Ltd Isoleret oligonukleotid og anvendelse deraf i nukleinsyresekvensering
CA2965500A1 (en) 2014-10-24 2016-04-28 Abbott Molecular Inc. Enrichment of small nucleic acids
EP3221813A4 (en) 2014-11-20 2018-04-18 Children's Medical Center Corporation Methods relating to the detection of recurrent and non-specific double strand breaks in the genome
EP3237616A1 (en) 2014-12-24 2017-11-01 Keygene N.V. Backbone mediated mate pair sequencing
ES2870626T3 (es) 2016-05-24 2021-10-27 Translational Genomics Res Inst Métodos de marcado molecular y bibliotecas de secuenciación
ES2898088T3 (es) 2016-06-01 2022-03-03 Hoffmann La Roche Inmuno-PETE
WO2017218979A1 (en) * 2016-06-17 2017-12-21 The Broad Institute, Inc. Unbiased detection of nucleic acid modifications
US10144962B2 (en) 2016-06-30 2018-12-04 Grail, Inc. Differential tagging of RNA for preparation of a cell-free DNA/RNA sequencing library
US10590451B2 (en) 2016-07-01 2020-03-17 Personal Genomics, Inc. Methods of constructing a circular template and detecting DNA molecules
JP7048105B2 (ja) 2016-07-15 2022-04-05 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 核酸ライブラリを生成する方法
US10011870B2 (en) 2016-12-07 2018-07-03 Natera, Inc. Compositions and methods for identifying nucleic acid molecules
AU2018212756B2 (en) 2017-01-27 2021-02-25 Integrated Dna Technologies, Inc. Construction of next generation sequencing (NGS) libraries using competitive strand displacement
CN110612351B (zh) 2017-03-20 2023-08-11 Illumina公司 用于制备核酸文库的方法和组合物
IL269431B2 (en) 2017-03-23 2025-05-01 Univ Washington Methods for targeted nucleic acid sequence enrichment with applications to error corrected nucleic acid sequencing
US11198865B2 (en) 2017-11-02 2021-12-14 Amanda Raine Splinted ligation adapter tagging
AU2019207900B2 (en) 2018-01-12 2025-07-10 Claret Bioscience, Llc Methods and compositions for analyzing nucleic acid
WO2019236726A1 (en) 2018-06-06 2019-12-12 The Regents Of The University Of California Methods of producing nucleic acid libraries and compositions and kits for practicing same
WO2020206143A1 (en) 2019-04-05 2020-10-08 Claret Bioscience, Llc Methods and compositions for analyzing nucleic acid

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103354840A (zh) * 2011-01-31 2013-10-16 欧凌科公司 核酸外切酶可实现的邻近延伸分析
WO2016058517A1 (en) * 2014-10-14 2016-04-21 Bgi Shenzhen Co., Limited Mate pair library construction
WO2016156529A1 (en) * 2015-03-31 2016-10-06 Qiagen Gmbh Efficiency improving ligation methods

Also Published As

Publication number Publication date
JP7537748B2 (ja) 2024-08-21
US11629345B2 (en) 2023-04-18
AU2019280712B2 (en) 2025-11-20
KR102767028B1 (ko) 2025-02-13
WO2019236726A1 (en) 2019-12-12
US20210222161A1 (en) 2021-07-22
CN112243462A (zh) 2021-01-19
JP2021526798A (ja) 2021-10-11
CA3100983A1 (en) 2019-12-12
JP2024161413A (ja) 2024-11-19
EP3802864A1 (en) 2021-04-14
US20230242907A1 (en) 2023-08-03
KR20210016560A (ko) 2021-02-16
AU2019280712A1 (en) 2021-01-07

Similar Documents

Publication Publication Date Title
CN112243462B (zh) 产生核酸文库的方法以及用于实践所述方法的组合物和试剂盒
CN106715706B (zh) 直接从未纯化的生物样本分析核酸的方法
EP4090766B1 (en) Methods of targeted sequencing
EP3485035B1 (en) Methods of producing nucleic acid libraries
WO2020132316A2 (en) Target enrichment
JP6971276B2 (ja) クランプオリゴヌクレオチドを利用する核酸増幅方法
US20190169603A1 (en) Compositions and Methods for Labeling Target Nucleic Acid Molecules
US11174511B2 (en) Methods and compositions for selecting and amplifying DNA targets in a single reaction mixture
CN115279918A (zh) 用于测序的新型核酸模板结构
US20260049303A1 (en) Di-Modal DNA Libraries and Methods of Preparation and Uses Thereof
US9428747B2 (en) Methods for amplification of nucleic acids utilizing hairpin loop or duplex primers
US20250305040A1 (en) Methods for Producing Circular Deoxyribonucleic Acids
CN118401675A (zh) 制备dna文库的方法及其用途
US20190284550A1 (en) Methods of depleting or isolating target rna from a nucleic acid sample
HK1219117B (zh) 使用拼装序列扩增片段化的目标核酸的方法
CN105247076A (zh) 使用拼装序列扩增片段化的目标核酸的方法

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant