CN112243462B - 产生核酸文库的方法以及用于实践所述方法的组合物和试剂盒 - Google Patents

产生核酸文库的方法以及用于实践所述方法的组合物和试剂盒 Download PDF

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CN112243462B
CN112243462B CN201980038046.3A CN201980038046A CN112243462B CN 112243462 B CN112243462 B CN 112243462B CN 201980038046 A CN201980038046 A CN 201980038046A CN 112243462 B CN112243462 B CN 112243462B
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ssna
ssb
oligonucleotide
bound
nucleic acid
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CN112243462A (zh
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R·格林
J·卡普
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University of California, San Diego
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2533/00—Reactions characterised by the enzymatic reaction principle used
    • C12Q2533/10—Reactions characterised by the enzymatic reaction principle used the purpose being to increase the length of an oligonucleotide strand
    • C12Q2533/107—Probe or oligonucleotide ligation

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
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  • Engineering & Computer Science (AREA)
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  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201980038046.3A 2018-06-06 2019-06-05 产生核酸文库的方法以及用于实践所述方法的组合物和试剂盒 Active CN112243462B (zh)

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US201862681524P 2018-06-06 2018-06-06
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PCT/US2019/035617 WO2019236726A1 (en) 2018-06-06 2019-06-05 Methods of producing nucleic acid libraries and compositions and kits for practicing same

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EP (1) EP3802864A1 (enExample)
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US20230014607A1 (en) 2019-10-09 2023-01-19 Claret Bioscience, Llc Methods and compositions for analyzing nucleic acid
WO2022076574A1 (en) 2020-10-08 2022-04-14 Claret Bioscience, Llc Methods and compositions for analyzing nucleic acid
GB2606158B (en) * 2021-04-26 2023-12-27 Wobble Genomics Ltd Amplification of single stranded DNA
JP7846205B2 (ja) * 2021-07-22 2026-04-14 深▲セン▼華大智造科技股▲ふん▼有限公司 ニック－連結ｓｔｌｆｒ
EP4457356A4 (en) * 2021-12-24 2025-10-29 Pacbridge Partners Ii Invest Co Ltd COMPOSITIONS AND METHODS FOR IDENTIFYING GENE FUSIONS

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