CA3100983A1 - Methods of producing nucleic acid libraries and compositions and kits for practicing same - Google Patents

Methods of producing nucleic acid libraries and compositions and kits for practicing same Download PDF

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Publication number
CA3100983A1
CA3100983A1 CA3100983A CA3100983A CA3100983A1 CA 3100983 A1 CA3100983 A1 CA 3100983A1 CA 3100983 A CA3100983 A CA 3100983A CA 3100983 A CA3100983 A CA 3100983A CA 3100983 A1 CA3100983 A1 CA 3100983A1
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Prior art keywords
ssna
ssb
bound
oligonucleotide
adapter oligonucleotide
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CA3100983A
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English (en)
French (fr)
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Richard Green
Joshua KAPP
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University of California, San Diego
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University of California, San Diego
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2533/00—Reactions characterised by the enzymatic reaction principle used
    • C12Q2533/10—Reactions characterised by the enzymatic reaction principle used the purpose being to increase the length of an oligonucleotide strand
    • C12Q2533/107—Probe or oligonucleotide ligation

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA3100983A 2018-06-06 2019-06-05 Methods of producing nucleic acid libraries and compositions and kits for practicing same Pending CA3100983A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862681524P 2018-06-06 2018-06-06
US62/681,524 2018-06-06
PCT/US2019/035617 WO2019236726A1 (en) 2018-06-06 2019-06-05 Methods of producing nucleic acid libraries and compositions and kits for practicing same

Publications (1)

Publication Number Publication Date
CA3100983A1 true CA3100983A1 (en) 2019-12-12

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Family Applications (1)

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CA3100983A Pending CA3100983A1 (en) 2018-06-06 2019-06-05 Methods of producing nucleic acid libraries and compositions and kits for practicing same

Country Status (8)

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US (2) US11629345B2 (enExample)
EP (1) EP3802864A1 (enExample)
JP (2) JP7537748B2 (enExample)
KR (1) KR102767028B1 (enExample)
CN (1) CN112243462B (enExample)
AU (1) AU2019280712B2 (enExample)
CA (1) CA3100983A1 (enExample)
WO (1) WO2019236726A1 (enExample)

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* Cited by examiner, † Cited by third party
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WO2019236726A1 (en) 2018-06-06 2019-12-12 The Regents Of The University Of California Methods of producing nucleic acid libraries and compositions and kits for practicing same
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US20230014607A1 (en) 2019-10-09 2023-01-19 Claret Bioscience, Llc Methods and compositions for analyzing nucleic acid
WO2022076574A1 (en) 2020-10-08 2022-04-14 Claret Bioscience, Llc Methods and compositions for analyzing nucleic acid
GB2606158B (en) * 2021-04-26 2023-12-27 Wobble Genomics Ltd Amplification of single stranded DNA
JP7846205B2 (ja) * 2021-07-22 2026-04-14 深▲セン▼華大智造科技股▲ふん▼有限公司 ニック-連結stlfr
EP4457356A4 (en) * 2021-12-24 2025-10-29 Pacbridge Partners Ii Invest Co Ltd COMPOSITIONS AND METHODS FOR IDENTIFYING GENE FUSIONS

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AU2019280712A1 (en) 2021-01-07
US12686864B2 (en) 2026-07-21
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