CN105247076A - 使用拼装序列扩增片段化的目标核酸的方法 - Google Patents
使用拼装序列扩增片段化的目标核酸的方法 Download PDFInfo
- Publication number
- CN105247076A CN105247076A CN201480027411.8A CN201480027411A CN105247076A CN 105247076 A CN105247076 A CN 105247076A CN 201480027411 A CN201480027411 A CN 201480027411A CN 105247076 A CN105247076 A CN 105247076A
- Authority
- CN
- China
- Prior art keywords
- nucleic acid
- sequence
- target nucleic
- group
- assembled sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 169
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 119
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 119
- 238000000034 method Methods 0.000 title claims abstract description 61
- 230000003321 amplification Effects 0.000 claims abstract description 39
- 238000003199 nucleic acid amplification method Methods 0.000 claims abstract description 39
- 238000013467 fragmentation Methods 0.000 claims description 26
- 238000006062 fragmentation reaction Methods 0.000 claims description 26
- 238000012360 testing method Methods 0.000 claims description 15
- 230000000295 complement effect Effects 0.000 claims description 13
- 238000000137 annealing Methods 0.000 claims description 11
- 238000001514 detection method Methods 0.000 claims description 8
- 230000009977 dual effect Effects 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 7
- 238000007689 inspection Methods 0.000 claims 1
- 239000012634 fragment Substances 0.000 abstract description 12
- 230000007614 genetic variation Effects 0.000 abstract 1
- 239000000523 sample Substances 0.000 description 20
- 108020004414 DNA Proteins 0.000 description 17
- 239000002773 nucleotide Substances 0.000 description 16
- 125000003729 nucleotide group Chemical group 0.000 description 16
- 108091028043 Nucleic acid sequence Proteins 0.000 description 13
- 238000009396 hybridization Methods 0.000 description 10
- 238000012986 modification Methods 0.000 description 9
- 230000004048 modification Effects 0.000 description 9
- 238000003752 polymerase chain reaction Methods 0.000 description 9
- 238000004458 analytical method Methods 0.000 description 8
- 238000006243 chemical reaction Methods 0.000 description 8
- 102000004190 Enzymes Human genes 0.000 description 6
- 108090000790 Enzymes Proteins 0.000 description 6
- 108091034117 Oligonucleotide Proteins 0.000 description 6
- 229940088598 enzyme Drugs 0.000 description 6
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 5
- 108091093088 Amplicon Proteins 0.000 description 5
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 5
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 5
- 238000009415 formwork Methods 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 description 4
- 108060002716 Exonuclease Proteins 0.000 description 4
- 101710163270 Nuclease Proteins 0.000 description 4
- CZPWVGJYEJSRLH-UHFFFAOYSA-N Pyrimidine Chemical compound C1=CN=CN=C1 CZPWVGJYEJSRLH-UHFFFAOYSA-N 0.000 description 4
- 102000013165 exonuclease Human genes 0.000 description 4
- 210000002700 urine Anatomy 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- LTMHDMANZUZIPE-AMTYYWEZSA-N Digoxin Natural products O([C@H]1[C@H](C)O[C@H](O[C@@H]2C[C@@H]3[C@@](C)([C@@H]4[C@H]([C@]5(O)[C@](C)([C@H](O)C4)[C@H](C4=CC(=O)OC4)CC5)CC3)CC2)C[C@@H]1O)[C@H]1O[C@H](C)[C@@H](O[C@H]2O[C@@H](C)[C@H](O)[C@@H](O)C2)[C@@H](O)C1 LTMHDMANZUZIPE-AMTYYWEZSA-N 0.000 description 3
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 3
- 108091028664 Ribonucleotide Proteins 0.000 description 3
- -1 cell Substances 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 238000013461 design Methods 0.000 description 3
- LTMHDMANZUZIPE-PUGKRICDSA-N digoxin Chemical compound C1[C@H](O)[C@H](O)[C@@H](C)O[C@H]1O[C@@H]1[C@@H](C)O[C@@H](O[C@@H]2[C@H](O[C@@H](O[C@@H]3C[C@@H]4[C@]([C@@H]5[C@H]([C@]6(CC[C@@H]([C@@]6(C)[C@H](O)C5)C=5COC(=O)C=5)O)CC4)(C)CC3)C[C@@H]2O)C)C[C@@H]1O LTMHDMANZUZIPE-PUGKRICDSA-N 0.000 description 3
- 229960005156 digoxin Drugs 0.000 description 3
- LTMHDMANZUZIPE-UHFFFAOYSA-N digoxine Natural products C1C(O)C(O)C(C)OC1OC1C(C)OC(OC2C(OC(OC3CC4C(C5C(C6(CCC(C6(C)C(O)C5)C=5COC(=O)C=5)O)CC4)(C)CC3)CC2O)C)CC1O LTMHDMANZUZIPE-UHFFFAOYSA-N 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000002777 nucleoside Substances 0.000 description 3
- 108091033319 polynucleotide Proteins 0.000 description 3
- 102000040430 polynucleotide Human genes 0.000 description 3
- 239000002157 polynucleotide Substances 0.000 description 3
- 239000011541 reaction mixture Substances 0.000 description 3
- 239000002336 ribonucleotide Substances 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- 102000053602 DNA Human genes 0.000 description 2
- 101150039808 Egfr gene Proteins 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 108010090804 Streptavidin Proteins 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 229930003756 Vitamin B7 Natural products 0.000 description 2
- 230000000692 anti-sense effect Effects 0.000 description 2
- XYOVOXDWRFGKEX-UHFFFAOYSA-N azepine Chemical compound N1C=CC=CC=C1 XYOVOXDWRFGKEX-UHFFFAOYSA-N 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- MCWXGJITAZMZEV-UHFFFAOYSA-N dimethoate Chemical class CNC(=O)CSP(=S)(OC)OC MCWXGJITAZMZEV-UHFFFAOYSA-N 0.000 description 2
- 125000004119 disulfanediyl group Chemical group *SS* 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 108700021358 erbB-1 Genes Proteins 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- DRAVOWXCEBXPTN-UHFFFAOYSA-N isoguanine Chemical compound NC1=NC(=O)NC2=C1NC=N2 DRAVOWXCEBXPTN-UHFFFAOYSA-N 0.000 description 2
- 210000003734 kidney Anatomy 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- YACKEPLHDIMKIO-UHFFFAOYSA-N methylphosphonic acid Chemical class CP(O)(O)=O YACKEPLHDIMKIO-UHFFFAOYSA-N 0.000 description 2
- 238000010369 molecular cloning Methods 0.000 description 2
- 210000003205 muscle Anatomy 0.000 description 2
- 230000035772 mutation Effects 0.000 description 2
- 244000045947 parasite Species 0.000 description 2
- 230000004224 protection Effects 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 125000001424 substituent group Chemical group 0.000 description 2
- 230000001629 suppression Effects 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 238000013519 translation Methods 0.000 description 2
- 239000011735 vitamin B7 Substances 0.000 description 2
- 235000011912 vitamin B7 Nutrition 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- XBYRMPXUBGMOJC-UHFFFAOYSA-N 1,2-dihydropyrazol-3-one Chemical class OC=1C=CNN=1 XBYRMPXUBGMOJC-UHFFFAOYSA-N 0.000 description 1
- XQCZBXHVTFVIFE-UHFFFAOYSA-N 2-amino-4-hydroxypyrimidine Chemical compound NC1=NC=CC(O)=N1 XQCZBXHVTFVIFE-UHFFFAOYSA-N 0.000 description 1
- BXJHWYVXLGLDMZ-UHFFFAOYSA-N 6-O-methylguanine Chemical compound COC1=NC(N)=NC2=C1NC=N2 BXJHWYVXLGLDMZ-UHFFFAOYSA-N 0.000 description 1
- ZAOGIVYOCDXEAK-UHFFFAOYSA-N 6-n-methyl-7h-purine-2,6-diamine Chemical compound CNC1=NC(N)=NC2=C1NC=N2 ZAOGIVYOCDXEAK-UHFFFAOYSA-N 0.000 description 1
- 102000007469 Actins Human genes 0.000 description 1
- 108010085238 Actins Proteins 0.000 description 1
- 108700028369 Alleles Proteins 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- HMFHBZSHGGEWLO-SOOFDHNKSA-N D-ribofuranose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H]1O HMFHBZSHGGEWLO-SOOFDHNKSA-N 0.000 description 1
- 230000006820 DNA synthesis Effects 0.000 description 1
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 1
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 1
- 102000016911 Deoxyribonucleases Human genes 0.000 description 1
- 108010053770 Deoxyribonucleases Proteins 0.000 description 1
- 108700039887 Essential Genes Proteins 0.000 description 1
- 102100031181 Glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 description 1
- UGQMRVRMYYASKQ-KQYNXXCUSA-N Inosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(O)=C2N=C1 UGQMRVRMYYASKQ-KQYNXXCUSA-N 0.000 description 1
- 229930010555 Inosine Natural products 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 238000002944 PCR assay Methods 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 108091093037 Peptide nucleic acid Proteins 0.000 description 1
- 206010036790 Productive cough Diseases 0.000 description 1
- PYMYPHUHKUWMLA-LMVFSUKVSA-N Ribose Natural products OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 description 1
- 108020004682 Single-Stranded DNA Proteins 0.000 description 1
- 239000003570 air Substances 0.000 description 1
- 125000000217 alkyl group Chemical group 0.000 description 1
- HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 238000000429 assembly Methods 0.000 description 1
- 230000000712 assembly Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 230000001427 coherent effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000013211 curve analysis Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 229940109357 desoxyribonuclease Drugs 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000002405 diagnostic procedure Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000000835 electrochemical detection Methods 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000001036 exonucleolytic effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 210000001508 eye Anatomy 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 125000001153 fluoro group Chemical group F* 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 description 1
- 229910052736 halogen Inorganic materials 0.000 description 1
- 150000002367 halogens Chemical class 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 229960003786 inosine Drugs 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 125000000468 ketone group Chemical group 0.000 description 1
- 210000002751 lymph Anatomy 0.000 description 1
- 238000011880 melting curve analysis Methods 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 230000000869 mutational effect Effects 0.000 description 1
- 210000001989 nasopharynx Anatomy 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- IJGRMHOSHXDMSA-UHFFFAOYSA-N nitrogen Substances N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 150000003833 nucleoside derivatives Chemical class 0.000 description 1
- 125000003835 nucleoside group Chemical group 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 238000011020 pilot scale process Methods 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 150000003212 purines Chemical class 0.000 description 1
- BIJRUEBMNUUNIJ-UHFFFAOYSA-N pyridine-4-carboximidamide Chemical compound NC(=N)C1=CC=NC=C1 BIJRUEBMNUUNIJ-UHFFFAOYSA-N 0.000 description 1
- AYKQFJLUQSGQHU-UHFFFAOYSA-N pyrimidine-4-sulfonic acid Chemical compound OS(=O)(=O)C1=CC=NC=N1 AYKQFJLUQSGQHU-UHFFFAOYSA-N 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 210000000664 rectum Anatomy 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 125000002652 ribonucleotide group Chemical group 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 210000003802 sputum Anatomy 0.000 description 1
- 208000024794 sputum Diseases 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 125000000446 sulfanediyl group Chemical group *S* 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 210000001138 tear Anatomy 0.000 description 1
- 239000005451 thionucleotide Substances 0.000 description 1
- 210000001541 thymus gland Anatomy 0.000 description 1
- 239000001226 triphosphate Substances 0.000 description 1
- 235000011178 triphosphate Nutrition 0.000 description 1
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 description 1
- 239000005418 vegetable material Substances 0.000 description 1
- 230000004304 visual acuity Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
Claims (3)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201361798984P | 2013-03-15 | 2013-03-15 | |
US61/798,984 | 2013-03-15 | ||
PCT/US2014/029893 WO2014145176A1 (en) | 2013-03-15 | 2014-03-15 | Methods for amplifying fragmented target nucleic acids utilizing an assembler sequence |
Publications (2)
Publication Number | Publication Date |
---|---|
CN105247076A true CN105247076A (zh) | 2016-01-13 |
CN105247076B CN105247076B (zh) | 2021-06-18 |
Family
ID=51537894
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201480027411.8A Active CN105247076B (zh) | 2013-03-15 | 2014-03-15 | 使用拼装序列扩增片段化的目标核酸的方法 |
Country Status (6)
Country | Link |
---|---|
EP (1) | EP2971134B1 (zh) |
JP (2) | JP2016512696A (zh) |
CN (1) | CN105247076B (zh) |
CA (1) | CA2904863C (zh) |
HK (1) | HK1219117A1 (zh) |
WO (1) | WO2014145176A1 (zh) |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5871911A (en) * | 1992-12-07 | 1999-02-16 | Wisconsin Alumni Research Foundation | Method of site-specific nucleic acid cleavage |
US20060057567A1 (en) * | 2001-05-23 | 2006-03-16 | Hong Soon G | Method for constructing a chimeric dna library using a single strand speific dnase |
US20100151477A1 (en) * | 2002-01-31 | 2010-06-17 | Richard Cawthon | Reducing non-target nucleic acid dependent amplifications: amplifying repetitive nucleic acid sequences |
WO2010080910A1 (en) * | 2009-01-08 | 2010-07-15 | Bio-Rad Laboratories, Inc. | Methods and compositions for improving efficiency of nucleic acids amplification reactions |
CN101889096A (zh) * | 2007-10-04 | 2010-11-17 | 联邦科学及工业研究组织 | 核酸扩增 |
Family Cites Families (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5585481A (en) | 1987-09-21 | 1996-12-17 | Gen-Probe Incorporated | Linking reagents for nucleotide probes |
US5378825A (en) | 1990-07-27 | 1995-01-03 | Isis Pharmaceuticals, Inc. | Backbone modified oligonucleotide analogs |
ATE515510T1 (de) | 1991-12-24 | 2011-07-15 | Isis Pharmaceuticals Inc | Durch dna-abschnitte unterbrochene modifizierte oligonukleotide |
US5928905A (en) * | 1995-04-18 | 1999-07-27 | Glaxo Group Limited | End-complementary polymerase reaction |
JPH10500310A (ja) | 1994-05-19 | 1998-01-13 | ダコ アクティーゼルスカブ | 淋菌及びトラコーマ クラミジアの検出のためのpna プローブ |
US6153410A (en) * | 1997-03-25 | 2000-11-28 | California Institute Of Technology | Recombination of polynucleotide sequences using random or defined primers |
US6949367B1 (en) | 1998-04-03 | 2005-09-27 | Epoch Pharmaceuticals, Inc. | Modified oligonucleotides for mismatch discrimination |
EP1381680A1 (en) * | 2001-04-25 | 2004-01-21 | Proteus | Template-mediated, ligation-oriented method of nonrandomly shuffling polynucleotides |
US20040241732A1 (en) * | 2003-04-11 | 2004-12-02 | Chu-An Chang | Method of generating long nucleic acid molecules of defined sequence |
US6878531B1 (en) * | 2003-11-10 | 2005-04-12 | Medical College Of Georgia Research Institute | Method for multiple site-directed mutagenesis |
GB0500703D0 (en) * | 2005-01-14 | 2005-02-23 | Bioinvent Int Ab | Molecular biology method |
EP2430180A4 (en) * | 2009-05-11 | 2012-11-07 | Agency Science Tech & Res | gene synthesis |
CN101899434A (zh) * | 2010-06-18 | 2010-12-01 | 南方医科大学 | 一种可连接多片段的重叠延伸pcr方法 |
KR20210056458A (ko) | 2011-05-04 | 2021-05-18 | 바이오셉트 인코포레이티드 | 핵산 서열 변이체를 검출하는 방법 |
-
2014
- 2014-03-15 JP JP2016503272A patent/JP2016512696A/ja active Pending
- 2014-03-15 WO PCT/US2014/029893 patent/WO2014145176A1/en active Application Filing
- 2014-03-15 EP EP14765053.5A patent/EP2971134B1/en active Active
- 2014-03-15 CA CA2904863A patent/CA2904863C/en active Active
- 2014-03-15 CN CN201480027411.8A patent/CN105247076B/zh active Active
-
2016
- 2016-06-21 HK HK16107182.5A patent/HK1219117A1/zh unknown
-
2019
- 2019-05-22 JP JP2019095840A patent/JP2019176860A/ja active Pending
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5871911A (en) * | 1992-12-07 | 1999-02-16 | Wisconsin Alumni Research Foundation | Method of site-specific nucleic acid cleavage |
US20060057567A1 (en) * | 2001-05-23 | 2006-03-16 | Hong Soon G | Method for constructing a chimeric dna library using a single strand speific dnase |
US20100151477A1 (en) * | 2002-01-31 | 2010-06-17 | Richard Cawthon | Reducing non-target nucleic acid dependent amplifications: amplifying repetitive nucleic acid sequences |
CN101889096A (zh) * | 2007-10-04 | 2010-11-17 | 联邦科学及工业研究组织 | 核酸扩增 |
WO2010080910A1 (en) * | 2009-01-08 | 2010-07-15 | Bio-Rad Laboratories, Inc. | Methods and compositions for improving efficiency of nucleic acids amplification reactions |
Non-Patent Citations (1)
Title |
---|
I NASIDZE ET AL.: "Construction of Larger- Size Sequencing Templates from Degraded DNA", 《BIOTECHNIQUES》 * |
Also Published As
Publication number | Publication date |
---|---|
HK1219117A1 (zh) | 2017-03-24 |
EP2971134A4 (en) | 2017-01-18 |
JP2019176860A (ja) | 2019-10-17 |
CA2904863C (en) | 2022-12-13 |
CA2904863A1 (en) | 2014-09-18 |
WO2014145176A1 (en) | 2014-09-18 |
EP2971134B1 (en) | 2023-10-25 |
CN105247076B (zh) | 2021-06-18 |
EP2971134A1 (en) | 2016-01-20 |
JP2016512696A (ja) | 2016-05-09 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN103328654B (zh) | 立足点引物双链体的组合物及其使用方法 | |
EP3350732B1 (en) | Method for preparing a next generation sequencing (ngs) library from a ribonucleic acid (rna) sample and kit for practicing the same | |
CN107429296B (zh) | 捕获、检测和定量小rna的方法、组合物与试剂盒 | |
JP6335918B2 (ja) | 制限酵素を用いない標的富化 | |
RU2732589C2 (ru) | Композиции и способы для количественного определения последовательности нуклеиновой кислоты в образце | |
US11130991B2 (en) | Method for highly sensitive DNA methylation analysis | |
CN107868816B (zh) | 确定未处理的样品中感兴趣的核酸的量的方法 | |
US11499180B2 (en) | Primer extension target enrichment and improvements thereto including simultaneous enrichment of DNA and RNA | |
CN107406842A (zh) | 检测基因组编辑 | |
WO2012097353A9 (en) | Methods, compositions, and kits for detecting rare cells | |
WO2007053491A2 (en) | Method and kit for evaluating rna quality | |
US20070048757A1 (en) | Methods for characterizing cells using amplified micro rnas | |
KR102648647B1 (ko) | 짧은 호모폴리머릭 반복서열의 개선된 검출법 | |
JP3909010B2 (ja) | 高度ダイナミックレンジを有する定量的多重pcr | |
JP2023175685A (ja) | 尿路微生物検出のための組成物、方法、およびキット | |
MXPA06012557A (es) | Controles de ensayo de metilacion de gen. | |
CN105209640A (zh) | 使用发卡寡核苷酸扩增核酸的方法 | |
CN105907878A (zh) | 基于高分辨熔解曲线技术的结核分枝杆菌耐药性基因突变检测 | |
JP7004570B2 (ja) | 基質分子 | |
KR20210080220A (ko) | 극소량의 희귀 단일 염기 변이체 검출용 프라이머 및 이를 이용하여 극소량의 희귀 단일 염기 변이체를 특이적이고 민감하게 검출하는 방법 | |
EP2585615B1 (en) | Highly sensitive method for detection of a target nucleic acid in a sample | |
JP2022516250A (ja) | 固相モログラフィーによる標的核酸の検出 | |
CN105247076A (zh) | 使用拼装序列扩增片段化的目标核酸的方法 | |
CN113302301A (zh) | 检测分析物的方法及其组合物 | |
KR102678676B1 (ko) | 인공핵산을 이용한 표적핵산의 검출 방법 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
REG | Reference to a national code |
Ref country code: HK Ref legal event code: DE Ref document number: 1219117 Country of ref document: HK |
|
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right |
Effective date of registration: 20240306 Address after: California, USA Patentee after: ARNOLD LYLE Country or region after: U.S.A. Address before: California, USA Patentee before: ARNOLD LYLE J. Country or region before: U.S.A. Patentee before: NELSON NORMAN C. |
|
TR01 | Transfer of patent right |