CN112029733B - 一株高裂解性溶藻弧菌噬菌体rdp-vp-19001及其应用 - Google Patents

一株高裂解性溶藻弧菌噬菌体rdp-vp-19001及其应用 Download PDF

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CN112029733B
CN112029733B CN202011015636.5A CN202011015636A CN112029733B CN 112029733 B CN112029733 B CN 112029733B CN 202011015636 A CN202011015636 A CN 202011015636A CN 112029733 B CN112029733 B CN 112029733B
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bacteriophage
vibrio alginolyticus
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杜新永
李霞
江守杰
罗成盛
李先胜
赵丹丹
张庆
刘玉庆
盖春云
马如霞
刘爽
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Rec Union Qingdao Bioengineering Co ltd
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Abstract

本发明公开了一株高裂解性溶藻弧菌噬菌体RDP‑VP‑19001及其应用,其宿主为沙门氏菌S6，透过电镜观察，该噬菌体头部为廿面体，且立体对称，短尾，噬菌体长度为78.889nm，为有尾噬菌体目短尾噬菌体科，基因全长74730bp，不具有毒力基因和溶原性基因，保藏于中国微生物菌种保藏管理委员会普通微生物学中心，保藏日期为2020年8月27日，保藏编号CGMCC No.19980。溶藻弧菌噬菌体RDP‑VP‑19001对水体中溶藻弧菌具有强裂解作用，具有较高的温度耐受性和较宽的酸碱耐受范围，可以高效治疗该病原菌在水产养殖中所引起的溶藻弧菌疾病，以及可以作为消毒剂，为工业化、规模化、程序化生产噬菌体及治疗溶藻弧菌引起的水产养殖动物细菌病提供了噬菌体来源。

Description

一株高裂解性溶藻弧菌噬菌体RDP-VP-19001及其应用
技术领域
本发明涉及微生物领域，特别是涉及一株高裂解性溶藻弧菌噬菌体RDP-VP-19001及其应用。
背景技术
溶藻弧菌为革兰氏阴性短杆菌，具有嗜盐性，无芽孢、荚膜，隶属于弧菌科、弧菌属，是一种常见海洋致病菌，广泛分布于世界各地的海水和河口处，在海水类弧菌中数量最多。溶藻弧菌是一种重要的伺机性病原体和人畜共患病原菌，在人体和养殖水产动物上能够引起各种不同症状的弧菌病，溶藻弧菌在鱼、虾、贝等海水养殖动物中是一种条件致病菌，当环境恶化和机体免疫机能下降时，溶藻弧菌病容易爆发，多发生在夏季，爆发时往往会造成水产养殖业巨大经济损失。同时，溶藻弧菌对人的健康也易造成伤害，可以引起伤口感染、食物中毒、中耳炎等疾病。噬菌体是一类以细菌为宿主的病毒，广泛存在于自然界，专一性、自我裂解能力强，具备裂解细菌和控制细菌生长的功能，可以调节自然界中细菌的消长，用溶藻弧菌噬菌体作为生物杀菌剂预防、治疗和控制在水产养殖过程中的溶藻弧菌细菌病，具有低污染、高效率的优势，在一定程度上减少人为活动对自然界的影响。
本发明通过致病性溶藻弧菌的分离，进而分离出一株高裂解性溶藻弧菌噬菌体，并研究了其生物学特性，扩充了噬菌体储备库为开发治疗溶藻弧菌的抗生素替代品奠定了基础。
发明内容
本发明的目的之一在于提供一株治疗溶藻弧菌病、且安全可靠的高裂解性溶藻弧菌噬菌体RDP-VP-19001，提供一株生产该噬菌体的病原菌溶藻弧菌，同时提供了一种该噬菌体的分离方法。该噬菌体RDP-VP-19001保藏于中国微生物菌种保藏管理委员会普通微生物中心，北京市朝阳区北辰西区1号院3号，保藏日期为2020年8月27日，保藏编号CGMCCNo.19980。
本发明的另一目的在于提供该噬菌体在治疗人体或水产溶藻弧菌病中的应用，RDP-VP-19001具有较高的温度耐受性和较宽的酸碱耐受范围，效价较高，安全有效，有利于工业化生产。
为实现上述目的，本发明提供如下技术方案：
一株高裂解型溶藻弧菌噬菌体RDP-VP-19001，其宿主为沙门氏菌S6，透过电镜观察，该噬菌体头部为廿面体，且立体对称，短尾，噬菌体长度为78.889nm，为有尾噬菌体目短尾噬菌体科，基因全长74730bp，不具有毒力基因和溶原性基因，保藏编号CGMCC No.19980。
进一步地，溶藻弧菌噬菌体RDP-VP-19001对水体中溶藻弧菌具有强裂解作用，裂解率达98%，为工业化、规模化、程序化生产噬菌体及治疗溶藻弧菌引起的水产养殖动物细菌病提供了噬菌体来源。
进一步地，噬菌体RDP-VP-19001在pH为 6-9范围内作用1小时，其效价在5.9×106pfu/mL之上，适合生长在中性环境中。
进一步地，噬菌体RDP-VP-19001在pH=12时，效价达到103pfu/mL，在pH=4时，仍有噬菌体存活，噬菌体的酸碱耐受范围宽。
进一步地，噬菌体RDP-VP-19001的最适温度为37℃，效价最高达到109 pfu/mL.
进一步地，该噬菌体在-80℃的条件下可长期保存，效价维持稳定；在70℃的条件下作用20min，效价维持在102 pfu/mL，依然具有生物活性。
进一步地，该噬菌体可高效感染溶藻弧菌，其最佳感染复数为10。
进一步地，该噬菌体RDP-VP-19001用于水产养殖环境的消毒。
进一步地，该噬菌体作为消毒剂用于水体环境消毒。
进一步地，水体中加入噬菌体RDP-VP-19001后，3小时内有明显的裂解效果。
本发明的有益效果体现在：
（1）本发明发现并分离了一种溶藻弧菌，并以该溶藻弧菌为宿主分离得到了噬菌体RDP-VP-19001，该噬菌体RDP-VP-19001对水产养殖环境中溶藻弧菌具有强裂解效用，为工业化生产噬菌体用于养殖环境中溶藻弧菌的防治提供了噬菌体来源；
（2）在本发明中，噬菌体RDP-VP-19001在pH为 6-9范围内作用1小时，其效价在5.9×106pfu/mL之上，适合生长在中性环境中；在pH=12时，效价达到103pfu/mL，在pH=4时，仍有噬菌体存活，噬菌体的酸碱耐受范围宽。
（3）该噬菌体在-80℃的条件下可长期保存，效价维持稳定；在70℃的条件下作用20min，效价维持在102 pfu/mL，依然具有生物活性。
（4）当水体中加入噬菌体RDP-VP-19001后，3小时内有明显的裂解效果，可作为消毒剂用于水体环境的消毒。
（5）通过对本发明分离得到的噬菌体的生物学特性研究，为下一步研发新的抑菌药物提供理论指导意义。
附图说明
本发明上述的以及其他的特征、性质和优势将通过下面结合附图和实施例的描述而变的更加明显：
图1为电镜观察本发明分离得到的噬菌体的形态示意图；
图2为本发明噬菌体一步生长曲线示意图；
图3为本发明噬菌体pH稳定性实验的示意图
图4为本发明噬菌体热稳定性实验的示意图。
具体实施方式
本发明使用从青岛的海参池污泥中分离的致病性溶藻弧菌BV-19002，通过实验确定BV-19002的致病性，以BV-19002为宿主，从环境中分离得到一株裂解性溶藻弧菌噬菌体，并通过全基因组序列确定了RDP-VP-19001的基因型。
下面将结合附图和实施例对本发明的实施方案进行详细描述，但是本领域技术人员将会理解，下面实施例仅用于说明本发明，而不应视为限制本发明的范围。实施例中未注明具体条件者，按照常规条件或制造商建议的条件进行。所用试剂或仪器未注明生产厂商者，均为可以通过市售购买获得的常规产品。
实施例1 溶藻弧菌的分离鉴定
从具有溶藻弧菌细菌性疾病的海参池的污泥中取样，采用无菌操作的方法，在TCBS琼脂培养基中划线，37℃培养19h-23h后，在培养基中呈现圆形且边缘整齐的绿色光滑菌落，直径约2-4 mm；然后挑取典型菌落在科玛嘉弧菌显色培养基上划线，37℃培养19-23h后，在科玛嘉弧菌显色培养基上呈现无色菌落，挑取典型菌落继续划线纯化4次，然后挑取单菌落接种于5mL的2216E液体培养基中，37℃、170rpm振荡培养4h，得到均匀浑浊的细菌增殖液。再通过16sRNA分子鉴定和血清型鉴定，确定为溶藻弧菌，将其中一株命名为BV-19002。
实施例2 溶藻弧菌噬菌体RDP-VP-19001的分离鉴定
本发明实验用污泥样品为2018年从青岛海参养殖场中的海参池中采集，作为噬菌体分离的样品。
（1）取污泥5g加入10g已灭菌的纯化水，10000rpm离心5min，取上清液用0.22μm微孔滤膜过滤除菌。取3ml滤液和3ml处于对数生长期的宿主菌培养液，一起加入到20ml高压灭菌处理的2216E液体培养基中，静置于37℃培养箱中，过夜培养，对可能存在的噬菌体进行增殖。取样品培养液3ml，10000rpm离心5min, 取上清液用0.22μm微孔滤膜过滤除菌。假设上述滤液中含有噬菌体。用2216E液体培养基进行梯度稀释，与处于对数生长期的宿主增殖液制备双层平板，在37摄氏度下培养19-23h。双层平板上出现透明的噬菌斑。
（2）从有明显单个噬菌斑的双平板中挑取噬菌斑透亮、圆滑、小的噬菌斑，置于生理盐水中，用移液枪经过反复吹打，用0.22μm微孔滤膜过滤除菌，用2216E液体培养基进行梯度稀释，选择可以得到单个噬菌斑的梯度制备双层平板。以上步骤重复4-5次，直到得到的双平板上的噬菌斑大小、形态和清晰度相同。
（3）挑取已纯化完成的噬菌体的单个噬菌斑，加入到2216E液体培养基中，同时加入处于对数生长期的宿主菌培养液，37℃、170rpm振荡培养6h。取噬菌体增殖液5ml10000rpm离心5min，取上清液用0.22μm微孔滤膜过滤除菌。用2216E液体培养基做10倍梯度稀释至109 倍，取107、108、109倍稀释度的稀释液0.1ml和宿主菌增殖液0.1ml制备双层平板，测定噬菌体的效价。噬菌体效价（pfu/mL）=噬菌斑数*稀释倍数*10。结果表明，噬菌斑形态、大小和清晰度一致，为典型的裂解性噬菌体。该噬菌体的效价可达109fu/mL。
实施例3 噬菌体的形态观察
运用磷钨酸负染法：将噬菌体增殖液ml（效价109pfu/mL）滴在石腊片上，将铜网放在上面10min后取下，以吸附噬菌体。在自然室温下干燥2-3min, 用２％磷钨酸水液染色,2min后吸干水分，自然干燥5min,用电镜观察噬菌体形态。如图1所示。
由图1可知，噬菌体RDP-VP-19001头部为廿面体，且立体对称，短尾，噬菌体长度为78.889nm，根据噬菌体最新分类与命名，属于有尾噬菌体目短尾噬菌体科。
噬菌体RDP-VP-19001全基因组测序及分析：采用Illumina TruSeq™ Nano DNASample Prep Kit方法构建文库；具体步骤为：
1. 以1μg DNA起始量建库；
2. Covaris M220超声打断DNA到300-500bp；
3. 补平、3’端加A、连接index接头（TruSeq™ Nano DNA Sample Prep Kit）；
4. 文库富集，PCR扩增8个cycles；
5. 2%琼脂糖胶回收目的条带（Certified Low Range Ultra Agarose）；
6. TBS380（Picogreen）定量，按数据比例混合上机；
7. cBot固相载体上进行桥式PCR扩增，生成clusters；
8. Illumina Hiseq测序平台，进行2×150bp测序。
实施例4 噬菌体最佳感染复数(MOI)实验
将噬菌体增殖液（效价109pfu/mL）用2216E液体培养基进行10倍梯度稀释，按照MOI值为100、10、1、0.1、0.01、0.001，分别取每个梯度0.1ml稀释液和0.1ml的宿主菌增殖液（106fu/mL）加至5ml的2216E液体培养基中，37℃、170rpm振荡培养6h。将培养液10000rpm离心5min,采用双层平板法测其效价，效价最高的组合为其最佳感染复数。结果如表1所示。
表1
Figure DEST_PATH_IMAGE001
由表1可知，当感染复数为10时，培养6h后，增殖液较为清澈，效价最高，说明RDP-VP-19001的最佳感染复数为10。
实施例5 噬菌体一步生长曲线的测定
在最佳MOI条件下，将噬菌体和宿主菌混合，37℃培养20min,10000r/min离心5min,弃上清，用适量的2216E液体培养基洗涤沉淀。加入40ml2216E液体培养基，37℃、170rpm震荡培养，从0时刻起每10min取一次样，10000r/min离心5min,上清液过滤，测其效价。计算公式：裂解量=裂解末期噬菌体效价/感染初期宿主菌浓度；结果如图2所示。
由图2可知，噬菌体感染宿主菌后的40min 内效价没有明显变化，表明其潜伏期约40min，感染后50-110min 内噬菌体的滴度明显上升，之后稍有下降，表明噬菌体裂解期约为50 min。通过计算，噬菌体裂解量约为196PFU/感染细胞，表明噬菌体RDP-VP-19001具有潜伏期短、裂解力强的特点。
实施例6 热稳定性的测定
将效价为108pfu/mL噬菌体增殖液分装到EP管中，每管中加入噬菌体增殖液1ml，分别在40℃、50℃、60℃、70℃的水浴锅中孵育10min、20min和30min，然后用LB液体培养基10倍比稀释后，取样品和宿主增殖液各0.1ml铺双层平板计数。由图3可知，随温度的升高和时间的延长，噬菌体的效价越来越低。但是在70℃的条件下作用20min，效价维持在102pfu/mL，噬菌体依然具有生物活性，另外该噬菌体在-80℃的条件下可长期保存，效价维持稳定。
实施例7 酸碱稳定性的测定
用稀盐酸和稀NaOH溶液调节生理盐水的酸碱度，配成PH值为4、5、6、7、8、9、10、11、12、13的缓冲液，再将配置好的缓冲溶液将噬菌体稀释成1*107pfu/mL，将稀释液在37℃下，水浴1h，用LB液体培养基10倍比稀释后铺双层平板。37℃倒置培养4-6h后计数。结果如图4所示。
由图4可知，噬菌体RDP-VP-19001在pH为 6-9范围内作用1小时，其效价在5.9×106pfu/mL之上，表明该噬菌体适合生长在中性环境中；在pH=12时，效价达到103pfu/mL，在pH=4时，仍有噬菌体存活，说明噬菌体的酸碱耐受范围宽。
应当指出的是，具体实施方式只是本发明比较由代表性的例子，显然本发明的技术方案不限于上述实施例，还可以有很多变形。本领域的普通技术人员，以本发明所明确公开的或根据文件的书面描述毫无异议的得到的，均应认为是本专利所要保护的范围。
序列表
<110> 瑞科盟（青岛）生物工程有限公司
<120> 一株高裂解性溶藻弧菌噬菌体RDP-VP-19001及其应用
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 74730
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 1
ccggtaaacc ggtacgtgaa ccttcaagta aagcttgaac catttcttct ggtagtgaac 60
tcggttccat ctgtgcagca cgagctagtt cttgtacttg gcgagtagtc tcagccggac 120
gtagttcgaa ctcaggatac ttaacgatac gttgctcaga tgcaaagctc caaccagtac 180
gtagaataac tgtaccttca tcgacacatt tacgcacata gcgatcaatg aaagatactt 240
tatcaatctt agtgttcagt tggttgttga gaaccaattc attcatctta gcagcatgaa 300
catcttccga agtaaccggg tcaatattga agatatccgg ggtattcaaa aatggttcag 360
agaacgatgt ataacgccat tcgttattac gtcgaataac ttttggctga attgatgaac 420
gacctgcttt ctttttctcg tcttcagact taatatgtag gttctcaagc cattcggcaa 480
cacgagcttt ctgtgcatta tgtggactgc gagccagttc cagatcatgt tgcagatcag 540
ttaaagacgg tggattcttc cactctgtct taatattttc cgtcatgggg tgtccctcat 600
taatgattaa tcgtatagga gtgtattatg tacgaaacac aagagcaatg gtataagcgt 660
atgtacgaag aaaccaaaga tccaaattac ctaaagcttt tgaagctcgt cacaaacgaa 720
gaagagaccc cgaaggatct ctctaatttc cttatgcctt ctgaataggc ttttgtttag 780
caggcggcac cttcccaaat ggatcttgct gccttgccca cttctcttga cttgaaccac 840
tcattatacc accactctat tttcacacgg tggaagcgga tactcacaat ccaaatagtt 900
tttaaataac tcttccaacg ttaaaggccc aactgctcca gtagtattta ctgtaccagc 960
acgtttgtat tgacgacgat tatccagaat ctctactagt agaatatctt ctttcaaagt 1020
gaagtcatat gaaccagaga gatctgtctg agttgaacca gacaaacccc aaagagaatc 1080
accgttagtt actggtgtaa ttcgaatctt agcattagca actgcattgc ctaaagggtc 1140
gctaagtact cctgtaattt gaaccatttc ttactcctaa acatttccaa aactaggata 1200
aatatcatag tagtttgtat caattacggg aagtctcggt gcagcctgtg gataccaagc 1260
tgtattagta ggtgccgcag tagactccgt tgctgccttc aatactccat tagcataggc 1320
taaccaagtc aaataaatga ttgtgcctaa tggtgcaccg ccttgggcct gaatgccacg 1380
aggagttaat ggggtagcat aacccgcagc aggaagtgtt gagttagcca aataagtgct 1440
gttatctggt tcaaaaatct ctaacactct gagtagtggt ctggtcgaat gaaagcgagg 1500
aactccatta gtatcataga gggccaagcc ccaatcacct gaaccaattg gtacatgttg 1560
tgccggtaca aatacataaa gatctagtgt atgggccaca cctcctgtac ttccatattc 1620
taattctaaa attgaaccac tagagatgat tgctgcatgt ttagcaaagg tacttgtatt 1680
tgtgttacta gaaataccaa acactaaagg gttccagctc atcggaatat tgaagattgg 1740
tgtactcgtc ttagtagtat ttgaaatagt aatagagaac ttgccccaat atagataagt 1800
atcctctgta ccatattgca cgtactgtcc tgcttgattg taaatctcga atccaaaagc 1860
cattatgaag ttctccgtgc ataaagcatg aacacaccac cttcagcatt tgcgttcgat 1920
gaagtattag ttgttgtata cgtcaacgtg ttacctgcta ccgatacgct atcgagatac 1980
aaagcatcgc ctgttcctcg tgagaaatca ccgaagtcat aagtgtattc aagtactccc 2040
tgtaagtcag gaattgtaat ttgatgtgtg ccaagctgca ttggaaatac ccctaacaaa 2100
ttgtaagggg cataatccac aatgatctct cggcctgctt catcataagt agctaagcca 2160
taagccatta taatctccca agtctaactc gtaagtttgc accatcatat acttcaatac 2220
gatcgcctac aatacgtagt gaaccaccag tggcttgttc cagtacttca agtgaaccac 2280
gatagatacc attattgaac tctgcgttac cattcacatc aatagcccaa cctgctgaac 2340
cagatacata gttaccagat ttgatctgaa tctgtgactg gtctgggtag atacggttct 2400
ctgtccaatc cttagcagct tgttccgcgc tatccgcagc tccatcggca tatgtcttgg 2460
cattgttctc cgcagtgttt gccttattag tagcatctgt agctgctgta ctaatagcat 2520
cactctctgc tgcatcagct ttagcctgtg cacctgctgg agtttcagca ccgatagatg 2580
aagtagttac gttactgttg tcgatactcg ctggaattga accaccaaga accaaagcat 2640
ttaagtaaca agtaccatcc cgatctacac ggaatggagc aaagtctgga tcagaagcac 2700
ctgcccagaa acgccagtat tggtaaacac ctgcactagc tgcatcatca ccattcatac 2760
cagcagtaaa tgaaccagaa cctgcaatga ttgtagttgc tgaactgatc tgattacctg 2820
taatggtatt agcaacaagt cgatcaccag agattgtacc tcgtgcaatc aagtcacctt 2880
gaataaccaa agcagggtct gaccaagtaa cagggctacc ttctggcgag ttacaacgct 2940
tcacttctga gttagtaacc aaagacgagt ctgagttatt cacgtaagtc aggtgatcat 3000
ctagtaccgg tgtacgtcca aagttattga taaaatcaat cgttgcctga gcatcactag 3060
ggaagatacc tgtaccgtta ttgatagtgt accaacctga accaggttta cctacgatgc 3120
gataaacaac actaggagag ccgggaacac catcagtgac agttcgatct tgcctccaga 3180
aatgagtatc aagctgagtg ccgccccaat cagtccagtt accatcaata gtctgagttg 3240
ggccataccg agattcttca aagattgtaa taccgtcttc accttgttct ccacgtccaa 3300
ttgggaaagg atcactccac gggccatcct gcacaccatt cacaaacact gctcgacgtt 3360
cccatttgtc agcagggtct aatactgtgt gccagttgta gataccatca atagagtatt 3420
cagagcgagt ttcaatagtc tcaccgtcat cacctacaat acggcccgga tctgaccatg 3480
ggccagcaac accatttgta acgatacgct ctctacggaa cttatcacct gacacaaatg 3540
gttcaggatg ccaagctaca ccgtcaggag agtattggta ttcaatgtag attgtatcgc 3600
ctgagtcacc gtctgaacca tcgaagcctc tgaactgata aggtagtgac catgtttgag 3660
gatcaacaac tccgttaaca ctgtagttct ctctgcgcca atagtgataa ggctgtactt 3720
cgtcttccca cgggccaagg ttactatctg agtattggta tacttggaaa atagtatcgc 3780
catcttccgg tggagtgaga tcaatcggtt gaccgttctc atcaagtagt gaaccaatct 3840
gtaactgccc attaacagtt actttgcctg tatcagtatc aacagtgagt accggactta 3900
cgtctgtctt ggatacacgg aagttatcta caatgatctc atagtcagaa gtagtaccat 3960
cgttggtatg ggttactgaa gtaacttttg aaccaatatc agatacataa gattcttccg 4020
aagagttact tagttctgtg tttttagtca cagagtttga accaaagttc agaaccaagg 4080
cttctttaat agctagggcc agttccttta acttagtaaa tgaaccagaa atagtactag 4140
ctgattcttg tcggcttact gtagtaccta cttgatctgt atcagttaga ttagctgtag 4200
cagtcgtctt agtttcaaca tcactgatct ctgtacctac atctgtatct gtagaaatag 4260
aggcagaaga tgtctcttta attgaaccag tagaagcaaa ctcttcagag taaacacgct 4320
cttggtgttg gatacgtgca gtacctgctt tcacttcatt ggttcgttgg acaccatgtt 4380
cagtacaatc ttccgagtcc caagtctgat ggatatcaac tgagtgggca tctggattgc 4440
ttacctgtac acgaaattct cgagtttttg tcagagaatc ttgaaccaaa gagttccacc 4500
aagttagcca tggttcatca atggtaacag tatctgttgt atctactcgg gttgaataca 4560
tcaagccttc ctcaagagta atactctgag gggtagattg gttcaaaagt agattaccgg 4620
ttagaaggaa ttcgtcacct tcagtattca ttacctcaag tgagtaaaag cctttgagta 4680
agtagaaatc ataggcaccg ttttcatccg tcatgaatgt tacagttgat cctttgatcg 4740
tttggtctgt gtgtaatgct gttacccgaa tagggtgatt aggcattggc acatctaagg 4800
gatcacggag tgtcccttga aaatgcacat ccatagcgtc tcctatagtg cttctaagtc 4860
agctttaaat tctgcgagtc ttactgctga ttctggagat aaacaaacgg agccatcact 4920
cagctccgtc acgttcagat tcgtcggcca agtcggtgta cttcttaccg gacttgtgca 4980
ccctgccacc gttagcaata gtggaagcag ggtcatcagc agcatctctc tttttcttac 5040
gattaattgc atttgcaatt gcctccaaga ttctgagtat gatgcctagt gttttcattt 5100
ggggtttccc ttctgatctg atttagcgtt ttccgcgccc ttatagttac cagcaatctt 5160
attcaaaatt accataaccc aatcaggaat cttctcagtc actgaaacag gtaatactgc 5220
tacaacgtga accagaagaa aagcaaccat tgctaccaaa cctaagtaag gtgcaacaac 5280
agtacctcct agcaagtccc atacgatttg aaccaattcg ttcattattt ccattcctct 5340
agtttagttt ttacgtcaaa ggatggacat gctttattag caccccaatc acgatgacct 5400
ttaatattct cttctttaat gccatataag ccagctaagt aagagatcat gtaacgtagt 5460
gcatcccatt gaacatcggt aaagttgtcc tctgggttaa gatcagagtc tacaccaccg 5520
accaaacaaa caccaatatt gtctttgtta tgaccgtaag tatgtgcgcc ttgtcgagtg 5580
actggacgac cttcttcaat tgaaccatcg cgattgataa cgtagtgata tccaatgtca 5640
ctccaaccat taccattcac gtgccaatca cggataactg cagcatttac ttttgcatcc 5700
ggttgtgtag ctgaacagtg cactgttaga tatttaggat tcatacaaac tccaataagt 5760
taaagcctcc gaagaggctt gttattataa acctagctta gcttcaatag ccgctagtct 5820
agcttctagc tcttggttct tagctttcag ctcatcgttc tcagctttta actgttgaat 5880
tgaaccaaaa gacaatgcgt gtagatcgtt accggtacga agaatagtct tcattccatc 5940
ttcacgctct ttaaccaagt gaggtagaac tttctccatc tcttgagcaa taaagccata 6000
ctcgtacttg tgaaccactt cgtcttgttc agactcctcg ttgtactcca ttacttcttt 6060
taagtaagtg tgtactggaa tctggttcac aattgaaagt gcgtcatcaa cagatttgat 6120
gtttgacttc aatcgttcat cagaagctac agtatctaca taagctttac gtgctgcatg 6180
gtttgctaat gtaggatctc ctaccaaggt taactgtact ctgaaagtag ctgaagttga 6240
acttactaca aaccactgat ttcctgccgt agataagtca ggagcaccac caatagccca 6300
acttccgtat gttttacctg cccaaatatc gctgccagcg ctacctgcat aacgaatgtt 6360
tacattagct tcaccatttg aacggtacaa gtctaattta gtagcaccag acgattctac 6420
actcaatgct tctgtattga agatagcttt agtacggtca cggtaaatac taagtgcacg 6480
tcttacaaca ttgttatcac ggatacttac atagtattca ttgttagctg acgcagcatc 6540
gtaacccatc tccgcaccat aagaaccgtt agattcaaaa caacggaaag ctgaatttac 6600
gtttggttgg tcagcataca aatccatata cattggagtt gtaccatagt aggcaatacg 6660
catttgatgc gattgtaggt aggtaaagtt taagttacca ccttcaggat agaagcgcat 6720
ggtattgcca ccttcaccta acgactgaat cttagcaccg gtgtcaccca tttgaaccat 6780
attataggca aagttagagt ctgtgcctgt cagtttaagt gcaggactga ttgagtgtcc 6840
ccacgaacca cctgctgcac cggatacttg gtatttatct gagatagaag taccactcag 6900
aataatagtg tctcccgcac ctgcatttaa ctgaacatta ccgtcatctc gtaatactac 6960
attagcgttt ccattatcat tgaaccacgt catgttctca ttgccagatg aaccaaaacc 7020
aataaaacca cgtcttgact cgtcggaatg ttgccactca aagtaagaac ttgaaccaac 7080
acctgctgca accgcatcac ggaatcggta tgcagggcca ccagagtgtt catggattac 7140
tgcacccgta ttacctacaa caaatctttt agatgcatca gccaatccta atggtcttaa 7200
ttcaatatta ttgttatcgg cagttacaaa agtacggtta tctgcgtatg catagaaacc 7260
atgctgacct cctgctcggg aaatactgaa gttattactg ccttcatact ccaccagacc 7320
actcggttga aagttgtagt aatctgtatc taaggaaaca tccataccat taggacgaat 7380
ctgaataccc gcaactgaac cactgccacc aatacgagta gaacctccac caaacatcaa 7440
cacgccctga tctgcaccaa cagattgtcg gatagtggta gtaatcacat tagtagtgaa 7500
gatcttatcg cctgagattg attgatcacc agaagtctta accacttcac cgtccaatgt 7560
gttgatctgg ttctgtagtc cagtttcaat agcatccact tgagtagttg tataagcacc 7620
tacatcagaa gcagttggct tgtactcagt agtgtataaa cgtgaccaag caccaattgt 7680
acctgtgttt aaatcagcag cacgaacatg aacctcatga ccaacagtac gagcagctaa 7740
ttgaaaaccg ccttgtgtag tctgcatatg tagtaatgat cgagtaccag ttaggccatt 7800
cacgttacta ttagataggt tcaagattcg gtgatcacca tccgcaaaca atgaatctgc 7860
atctgaaatt acagactgat ctgtggcaga aattttacta tcaatatcag cctgtaatgc 7920
aggaactaca gccaaagtgt cttggtaaga ttgttctgca ttcgctgcac tggtagctgc 7980
gttagtctca cttgtagccg cattgcttgc acttgttgct gcatttgttt cgctagtcgc 8040
ggcattggct tcagaggtag cagcctgact tgcagaagtt tgagctgcac tagcactatt 8100
agctgcatta gtctcagagt tacttgctgc aattgctgaa ccagaagcat cagaagcact 8160
taacgcagca ttagtttcac tattactagc attcgtttca gatgtagctg ccgcattagc 8220
agaagccaaa gcattagcct cagacgtggc tgcattacta gcggatgtcg cagcattcgt 8280
ttccgaagtt gctgcattag tttctgacgt tgcagcgttt gtagcacttg tagaagcact 8340
tgacgcacta gctgccgcat tggtttctga agtagaagca ttgctcgccg atgttgcagc 8400
tgcttgttca ctcgctaaag catttgcctc agaagttgca gcgttagatt cagaagtact 8460
tgcagccgca gcacttgcag cagcattcgt ctcagagatc tgtgcatcat cacgtgccgc 8520
taccgcatcg ttagccgctt gaatagcagt ctgctcaggc acatcccaag ttgcaccatt 8580
ccagaaacgt agatcgttag tagtggtgtt ccagtaggtt gcacctactt gtaacggatt 8640
accatcattg tctactgtag gatcagtcgc tttggcacct aagtattgat cgttaaagtt 8700
atcaaatgca gcctcggcat ccgcagcata accagccgca actacagcag agtttgccgc 8760
agcagattct gacgcacccg cattagcttc gcttgtagca gccgcagaag cactgttagc 8820
agcgtttgtc tctgaagtgg cagcattgga ctcactaaga gccgcagccg tctcagatgc 8880
cttagcagcg ttctcagagg caagagcatt tgcttccgat gtagcggcat tggtttcact 8940
cgttgcagca gcatttgcac tagccagaga gttagcttcc gacaaagcag cattttgctc 9000
agataatagc gcagcagcct cagacgattg ggcattagtc tcagcaagtt cagcagctac 9060
ttgagccaag agagcagcat ctcgtgcagc aacagcagtc tgtgcagctt gaaccacagt 9120
ctcttcatcg acagaaaccc agatcatttc atctgcatcg taatagtcaa gagtattctc 9180
ggttgtattc aggtagaagt tacctgactt tagtggttca ttattccatc cttcagtagg 9240
tggagcagtg aacgcaccta agtataacga aattggttca ataccttccg acacagtaat 9300
cacttcttcg atatgctcag caacaatacg tacattgtca taagcagtac cgatgtgacg 9360
gttcaccatt gaattatgtt catcacactt attcacactt ggtttagcag gcattataca 9420
aatcctcttt gttcaagttt caaattagtg ttgttgttgt agttgttaag tacattacgt 9480
cgttcgatct cttggcagta tgcttcatac ttctgcatca tttcaagtga acgggaaact 9540
gcaccggcat cattactacg cgcatacata cgtgaagcaa catagaataa aactggttca 9600
atacaataat cagggatatc aatctctgta gttgcaggat cagcatttgg ttcaatttta 9660
ggatgattgg cacggtaagt tagaaacacc gtattatcat cagccggatt aacaacctgt 9720
aatgtgcgat agttgttgag ccagtagctg tcacagttat tctcatcgtt caatggaata 9780
ggacaaccta actcatcata tacagcagta atgcccaaca catcatcaat gaaaggacgt 9840
tctggcgtat caatgatgta cttaattggt tcaagagagt tatcgttaga taacgcatag 9900
tctgaactca attcataaaa cgataacgtt tcgtacagtt gaagtgtcaa ttctcgatca 9960
cgcaatggta gtcgagtatg gatagccgtt aaagccttgt tcaagtgtga aacaatagca 10020
gggtaatctt gcgcagtgat tcctgtcatt ggttcatcaa tatccccagt aacagcaaac 10080
tgtgaaagct caccatactg gagatcttga aatagttcct gtaataaaat catgttaact 10140
cctattgatc aaaggagttt cgattataca gtgtaagagt tcattctgtc attaaggaag 10200
tcatctggtt catcccaata gtagacatcc gattgatcat cacgaaccag cataccatta 10260
gcagcaggct taaatggctt cattacagat agcattgaga tggtatccga gaagtcatcg 10320
tgcttagact taaagccgtg taccgataga agcgtaagct catccataca ttgaaccatt 10380
tctggcttct tggctaactc tttggggaac atgattttgt ggttcttaaa cataggaacc 10440
atagtgttga aacgttggaa cttattagtt acaggacgga taccctcttt gttagagtta 10500
ttgtccgaag ctaaagtgaa ccaaattcca gtattaccca ttcgctcttt gatgtaatgg 10560
atgaaaccac cttgctgacc agtaacctcg acacctactt tgattggatt gtacatttga 10620
accaatctga atagatcatc taagttgtag tccatagttt gacgacgaca gattccatct 10680
acccacatcc agtcaccgtt attattgtac gcccaaacag agataaccga gaagtcggca 10740
gactgtttat cagacgttgc aaagtcggta gtgatgtaga agttgtaggc gtgcatgtta 10800
tttagtacgt cattacggtt gtaccacacg atatcgccat catggatcaa tcggtcttct 10860
tccgacatga tctgtagcat aagttcttgg ttaaacgctt cggccttacc agacttaact 10920
gctttggtgt acatcttcat tacagcatcg taatcaaaac gatcctccca tgaacctttg 10980
aactcttcac gtgtacaagg gaatgtttct gctactggat atacgtttac gttgtaggca 11040
ccagattcaa ctgctttgta cagtggatct tttgcgttaa agggtgtccc tgaccaaatc 11100
actttacgac gttgagggtg taacgcatag tcgattgctt tgttgatcgt atcttctaca 11160
gattggatta ctgtcggtga acgtgcatcc tcatccgaga tcaagtcatc gagcaaagct 11220
agtacaggac gtgaaccatt ttcacgagta ccacgaacac cagtcttagc accgtggccc 11280
gatacaacga aagatttacc ttcaatattg ataaactccc aacggatgtc agtaagtttc 11340
attgttggta cgtacttttg caggaatgct gagttgttat agcgatactc aagtgcctta 11400
cgcattttct tcacgccgtt atccattgag tcggatacat acagtgcata aggtactcga 11460
ccaaatcccg gcagctcatt aaataccgcg agatacaaga tcaggtactc tttcagagta 11520
gacttagcaa aaccacggtg acacatgttc aatgtatcta ggccgttatc ctgtacaaag 11580
ttatcgagca ttttaaagtg tactggtgga gttttgttct cattctcacc accatctaca 11640
agcttcatga agttcacaaa ctcaagtgcg aactggctcg gcttatagtt atcaattgcc 11700
gtatagtcca cctcgttgag gtagtcttga acggttttct taatttcgtt ctctagttct 11760
tccattttag cctctaaagg ggagtttcct cccctgattt agatgagtag tagagttgat 11820
actacgatcc cagattaata agaggaatct tcaactcctc gccctttggg gtttgtaata 11880
caagatgttc ctcgttcgag tatacataca gaagtgctcc cttctttaac gagtcagcca 11940
actgtttaac ctcgctaatt tcgtcagaat gtagttgcag caatttgttt gtactctcat 12000
ttgcaagaac aacttgatcc agcttgctat taatatttgc catatttgta gctgctgtcc 12060
acacccccga agcaattgta agtaatccca ccactaacgc gacaagagta ccaaactttt 12120
ggagatcaga catatcttca ccaagttcct caccctgcta taaaaaactg aaccaataga 12180
gcgcagggat aactctaaag gaacagttct ttaattgatt ggtctgaatg cgatgagcaa 12240
atcatctaca gtaagtgtac caacgaagtc tttagcgacc tccatgtaga tgacatcacc 12300
cggattaaac agaccaaaag catttagtgt ataaccaata gatgaagtag tttgacctaa 12360
tgatcgtcca gcagcatatc gcttagttga accagtcatt acaccagatg gttcaaccat 12420
tcgaacaaac aagtggtcgg tatcagtagt actatccgaa tccaacagac cacctacgtt 12480
gatctcatag ccctgcgttt ttgaaccaat ataggtaaac gttgcactat taacatcgaa 12540
tgtccaattg ttcgaatatt caataggagc actaagtgta ttgatcagtg caagagtcca 12600
agtaacgttt tcatcagtaa cggtttgtac agcgttattg ttaccataga tgatatctac 12660
acttaggtta tctttatact gctcatactc aatacgagac acgccctcaa gccaaccacc 12720
aaagatacgt aagtatggta ggccatcagc aggagcatct actttaatag gtgaagcagt 12780
attaactgca atttggttca aaacatcaaa gtaagaaggt acaccttctt tacgagtctc 12840
aatagagaac actgcattgt ttgaaccatt tacaagaata cttagatctg catggtgtcc 12900
gtggcttaac gccactggag tgaatgcgga taaagttcca ccaggaatgg gaatccatcc 12960
tgaaccagta cctgtaccta caacgactgt agctccagca ggaatatcac cgggcacaaa 13020
cgtagtaacg tcggtattac cttcgaaatt taggggatac aggccatccc cttcaatcac 13080
ataattagcc attattgatc cctttgtaag ttttatatgc caagttccag ataacatact 13140
ctaaggtttt tagtttatgt tttggcttct cactgaaagg atcatcttta gttgcaaaat 13200
aatagccatc ttctttttca agaatgaagc gtttctcttt tagatgaata aagtgctcat 13260
aaacactttt ctccatctca gcccattcat taagtgtgaa cttattcgac atcttcaact 13320
tctccttcaa tgatcactga gtgcgctact tctttagcat tatgcattcc agattcaata 13380
gacttctgtt gtgcaatagc caagtcttgt accgcattgc gtagatcttt gagttcagag 13440
ttgtccttgt gagagatttc caattccatc ttgttagtct caggcggctt caagtgcgtt 13500
aacacactgt tcgctgcatc agatcgaacc ttctcggaat tagcattcat catcaagtct 13560
acttgaacgt tcaaagcctt ttgccttgca tcttggttca atacgtgaaa aggaaccatt 13620
gattgttccc agatcaaatt gactagcttg gacttattgt acgcatggac gtattttgca 13680
atatcagcat cagagacgcc agacgttttc cagttagtgt acttgtcagg gaaggtctta 13740
atatatgact tgatgttagt ctcaccacat aacttgtacg agacataacg cacagcatcg 13800
agataggaag ttagtttaaa cttgccttta tcgagtacat ttgtataaga caagaggttc 13860
tcacgataga tctcacgcat ctcaggatct tgaaccaatt ggttcacagt atctactagc 13920
tcttgatcta cttttcctct aatctgtcta ggcaacgctt cagacaacgt tgttactgtt 13980
agatcactca ttagtcttac cttagttgta tttggttcag atagtatatg agttatatag 14040
gagttttaaa agtgttgact gaaaagagca gatgcccaca ttgcggatgg agagattttg 14100
aaatgtgtga ttatcccttc gacagcagtg gttcaaaata ccgagcttgg tgtccgaagt 14160
gtgagaaccc ctttgaattt cgtgaaccaa ttttaacctt agaggaaacc tatgaaatca 14220
acgaaacata acactgtatt ttttgtattc ccttgtggag ccgtgataaa acgttttggt 14280
tcaatgcgag gcatgcgtat aaacggcagt agaccctact gggctgtatt ccacaaaagc 14340
tttgatcctt atctaaaggg cagactacac atgattctcc atcctatgca tggtatgaag 14400
atggagatac ctgacgagtt tgaaccatta atagataaat tcattcaacg tattaaactg 14460
tagttaagag gagccttcgg ctcctttttg cgagtgtagt cacaataatc acttatagag 14520
gtagtcatgc ctgagaaatt caatacaatt agagcttcac ctatgtctct tatagagaat 14580
caggagaaag aagagtgtct aacatattat caaaaagtaa ataaatacaa taaagaatta 14640
gcagaatacc taaatatatt aaactacgac gaaaatccca aagaaatgaa tgttctcttc 14700
gacctaatag atcctcctaa agaggacgag catcttgtca gaacactaat cgcattaagg 14760
ttgatgccat gaatgcatac caaaagatac agtgcttcga gaagacttac caagaatacc 14820
gagacttact tggacaagac gtaagtgaag aagaattgtt accccttatt aaagagcaat 14880
accctaacga tcaaagtcta gtccaatggt ggcatgataa tcatgtcaaa ctacctaagt 14940
ctccatttgc cccgatgaga cacaatggac tacttgatta aaatctactg gaaaagcacg 15000
atcacaggaa gccgatcttc tactgcctat atctcaccta agttggtgcc ccagatactg 15060
aaccacttaa agagcaacgg taaagtgcct actggattca aaaccagaga tcagccgtac 15120
aaggaaattc ctaatgaata ccaccaaact gaccccaata tcaactttat tagtcccgac 15180
ttttaaccaa aagtttgaag attatggttc ataccttgaa accaaaataa ccaacgatca 15240
ggttcgtaac atcctatcca atgaaggtta ttccagttcc atcataaatg attacatcca 15300
ctatcgagat caactagaag ctgctcgtat ggctatggaa gcttcagtga tatctggttc 15360
aatgatcata ggtaactctc ttgttgtaca tgggcctgca cctactataa aggctatgcc 15420
atgaaatcac gttacagcgt tttactaatg cacgcaaagc atcagatcga cgcacaagag 15480
aactccttca tccctattaa actattcgac atgctaattt atgaacgtgg gtttatgggt 15540
atgactcgaa tgctggatct attcgaaagt gaaccacaag cttttggaat ctttgtaatc 15600
ggactaacag gagatctcga tgaactcacg cctcagtaaa ctagtaatag ggtctcgcga 15660
atcagatgac cgaggtatgg tagcgtgcca tcaacttaca cacaaaacgt gcccttctac 15720
tttaaccaaa gaatctgtgg actgctctat atgtgcatta gacgaactaa gcaaatttaa 15780
tagcttatta ggagatctcg atgaaatgct ctagatacct aaagcttgcc gattatctag 15840
aaaatggttt aaaccgtgaa atacagggtg atcttacctt cagtaaattt gaaatggtcg 15900
gtgaacagtg gctaagtgta cgtaaacctt ccactatgaa ccagtggttc aaaatagata 15960
acctacttaa aacaatagga gagatagatg tctcgattaa atacgctgtt tgaacatgct 16020
aaagaagtaa gaaagcatgg ttcacatgga agaggttaca ttcctaacga tttatacgaa 16080
agaaagttaa ttccgggttg tagtaaaata aacatggata cctattctct tgtaaaagtc 16140
atgattgtac taatcataga ggaaggtttg aaccaatgtc tcgattagga actcttctaa 16200
cgtcaggtgc tcaacttggc cctgaataca gagatactgc tttccatttc cttggcctca 16260
aggtcgtata tgtgaagttt ggcctacctg atgaaccact tgtcctgaca accaccatat 16320
atactactaa attgatatgg gagcttaaaa aactatgtcg agattagacc taatagccaa 16380
aggagttctt agaaaccgtt ggttactcat aatgggcgag gtagatctaa gctattcaaa 16440
cgtaaatgtt tacttacctt gccaaattgc cagaaccaac gaagaacata gccgtatgat 16500
ctgccgtgta cgagcagaag tcctcgataa actcaaacca tttaaaggat atctttgaac 16560
catgtccaga ttagacatag tactgcgtaa tgctgggaga ggtagtcgcg tttgtaccta 16620
taaaccctgt ccatctacag gcacacgctc atattactgt accgattgcg cactttctta 16680
cacaaacgaa gagagtagaa aagaactctt taaggatcta ctagcatgtc acgtctagac 16740
accttcttac acaagaccaa agagcttagt cacactgatt ctgtgctgat attcgctcaa 16800
aaccgactat tcaacaatgc aaacagagat ctgttcatta gtggactgcc tttaattacc 16860
cgagctacta tgatcaagat actccgcgca ccatataagg taatgttatg tctcggctta 16920
gtagactttt agaaaatccc cttaattata agaacacctg caacgtattc gaaaaagatg 16980
agtacacctg taaggagtgt gtatgggagg atggcgtatg ctgccaaaat cctttgtatt 17040
ttagatctga cctagaggaa accgttgatg gaattaatcg acttacctga tcatctgatc 17100
actaaagtca acgtaaatga atacgaagct attcagacaa tcgttgcaga acaaaccaaa 17160
gaatactcac agatcctcga tacaaatgag gctgaagagt tgtacccaat ggttcaagaa 17220
caactaccca accttgataa acaagtagca ctagaagtac ttcgtaccat cctaccgcct 17280
atagagaatt gaaccatgtc acggatagat agtctgaacc agatggcatc cgatgcccag 17340
aataccactt cagttgagcg tcatggtgtt atctgttttg tattctttgt agtgaagggc 17400
ggctgggtag taaatgttgt gaaccaacaa ggtaacatga taggtgcact cccaattaac 17460
cgactaagag aactaactaa tgtctagact acaaactgtt atatcagctc acgcgttcta 17520
tatggttcaa cgtgaacact ttccggggcc acgcacgggg atgagtcata cccttttcaa 17580
caaacgctat ctacacacta tttttccaga gatagaaacc aatgacctga tctcaaggat 17640
agcgaatagc tacgataacc tttacttaat attggagaag ttatgtctag actagtaacc 17700
cttgaacaga agcacgatgc tagattacgt accggtggcc tttgtgacat acgctgtcct 17760
gaacaagcac aagataagac accttacctt atatttgcct atggttgtac agactgcgtt 17820
tactcttatg aaaacagaaa ccacttcaga ggaatcactg aatgtctcga ctcactatcc 17880
ttctaacaac ccgtacctat tgtaagatct ttgagagaac acgccgagag tgtacagggt 17940
gcccttggga ctctgatgta gataggtgtg aaatacccct tgttaactct aataacgtac 18000
aggaagtatt gaaatgtctc gaataactac actagaagaa caccacaaaa cctataacac 18060
aaaacgcttt gactttccta tcggcccagt cacctaccta aataactatg tggttcaaaa 18120
tacccctttt gatcgcagtg agcaaaccct cgcaagaaag aaccacgcag gagcagtatt 18180
cttcgactta ccaaacgtag tggagttgat atgtctcgat tagataaact gtatgaaggc 18240
gtgcctacct ttggtttaac actacaccgt aggtggtata aatctgtaac gatcatatac 18300
ctaaactttg aaccatctat taaactgaaa tccagtatga ggatggcatt aaaggagtta 18360
tcggaatgtc tcgactaact atattagagg cgggagtgac ccaaggagag ataaccctct 18420
acctacaacg agctactgcc aaatacaaaa caaccttcat actcaaaaac gcatttgata 18480
gtgctccctt caaaaaggtt cacgcgctat tcagagtaaa acaaggatta ctacgatgtc 18540
tcgactagat aaactactgg atgactttga ttgggcattt atgcaatcct tcatggaacc 18600
aatactagct aagtatgacc gtacagctgt aatcaattta gcaatagata ctactcgtcc 18660
tcccaaatac tcacttaaaa ggggaatact cgaatgtctc gactaagtac acttatggtt 18720
cacttcaaag gatatcgcgg tacagactct gggcctatga ctcatctaag cagaaaggta 18780
ttcaatggga actacataag cgacgtgtac cctcacatga ccatctatga taaacgccac 18840
atagttgaaa ccttccttaa actaccacga gtattggagc aaatatgtcc agactaacta 18900
ccctacttca agccagaaag gaggcagagg agacacttaa cttttcctac ataatgcgta 18960
tacgcctctt taatggtgta ggtatggtag ataagttctc ccacttccca aacatacaca 19020
taatgtacat ccgcagggca tttgtatcag cagctttcat tatactgaaa ttgaaccaga 19080
gagaaccaag atgtcacgac taaacacact ttaccaacat gcagctgatg tcacagcaga 19140
tattaaacct gtaacccaat acggtacagt tccagttgct cagtatgact tggttcggaa 19200
ccaaggaaat gggataacta gcagaatctt agtacgtagt attgaacacc taatctacga 19260
taataatttg gagaaactat gtcacggctt actcgactaa cagacccaaa tgaaccagta 19320
gtgattaatc ctaccttgga ctacccctct gtacaaagtc acccttatga agtcttatct 19380
gcatttgtgg ttcactatag cgataaagag gggtcttata ttagccgtga gatgttactc 19440
ggtacggtgt accttatagc taaaagtagg gaggaagtat gtcacgtcta gatactcttt 19500
taagctatga aagtgtggtt cacattaaaa caactctaca tgtgccttac ccttggctta 19560
gtgcagacaa gtttatgcac aatcaatatc acttctacat acaccacacg gataccacta 19620
agcatctatc ccaagatggg atacttgctc tagttttttc acttatgact tgtgaaccac 19680
ttttataggc aggtttttca gaattttcat aatttagtat gagtgtaata ctacccttct 19740
ttgtgttcgc tgagacgacc tacccccccc ccatagcagg atcactcggt aagtaatgct 19800
tcgcatctgt ccggcattaa tcaaccaacc aagaggattc aactatgtca ctattcaaag 19860
taatcgcacg cttcacatca accattgata atactctagc aatcgctgag aacatcactg 19920
ctgctggtgc taaagcttca gagtcatcca tcactctagc tgagaacttc gacctagaac 19980
agaaggctaa gctcaagcat aaacaagagc agatccaagc taacatcaag gcaggtaagt 20040
tcgacctatg attctatatc acggctatct gttcttctca attccagctc ttatcgcgta 20100
catcaaggaa gctccgtaag gggcttctag tacctaacac aacacaacac agagatagac 20160
tcatcctaag tagaaagagt tcttatagag cagcacagtt ggaattagag tgtattctat 20220
taatcttgct tgttcctcat actctctacg tgagctaccc agaactcata cctgactcat 20280
acaccaagcc atttcatgct gcttcgcagc taagcggatg caataatgta tctagaactt 20340
attaacctta ttggagaact atcatggcac aactagctaa ctcaactgaa tcaacttcac 20400
cacgtcgtaa ctctaaagag cagcaaccag ctcttgctta catcaacatc ggtaagaagc 20460
taccatcaga gacatctgaa tctggtgagt acatgcttaa tccattcttc ggtatcacag 20520
tcactgctga tgctcagaag actcgtttga ataagcaaga gaaatcactg tacgcagcag 20580
tacttgctaa gattgaagaa ctcaaagcaa atggtggtgc aggcaaatct cactcaatca 20640
acttggacat cgagctgtac attccagcag atgagccagc tgaagttgaa gcaggcaatt 20700
tcgacctgta attatcattg gtggccttcg ggtcaccttt ggcttttgaa ccacaaagat 20760
agaatgggct taaaatggat atcatctaca cttgtcagca ttgtcatgca gaatcaacca 20820
gtactatttc aggtagtgag atagaacacg gagctacata ccatcttaca tgctcatgtt 20880
gtgataaaga atcagaacta atgctagcca tttatccttt aaatgaacca caaagataga 20940
acaggaataa accatgtcta aatcacagtt aattaagcaa tacgcagcta aacacaactt 21000
agaatgtatt gatatcaaag cacctagtaa catttacgat aaactagcaa gtgtcgtaaa 21060
gagcaccaaa attgaaccaa aatggcaaat gcctgatgga ctcacaatgt tcacacgtct 21120
tactgctaaa cagcaattaa gcgatctaac tcgtgagtat atggttggac aacttaaatt 21180
aagtgcttat gaaccactat cagatgaatg gtacacaacc attgaatatc tatctgacct 21240
aggcactgac atcattgaat tgaaacaaga aattgagaga tttagctaat gtggaatgta 21300
tgttttgaac cagagtggat cagtgaagaa gagatggaaa tcattgaagc actcactcgt 21360
gaaattgaac cacaacttca agaacttagg tttgaggact agttatgaaa gtattagtag 21420
caggtggacg tgacttcacc gacacacaag taatgactga ctacatcaat gagctaatga 21480
ctaaaggcaa actacctgaa gacatcgact tagaactggt atgtggtatg gcagcaggtg 21540
cagatatcac tgcatatcat ttgtttaaaa acaatggctt agtagtacat gagtttccag 21600
ctgactggca tgacatgact gaaccatgca tacgcaaagt caacaaatat ggtgtgtaca 21660
atgcactagc aggcatgaaa cgtaacagtg aaatggcaca ggaagcagac gtgttaatag 21720
cattctggga tcgtaaatcc aaaggcacaa gagacatgat ctctcaaatg acacgtctca 21780
acaaaccaat attcatctac tactactaga gagcttcggc tctcttttag tttgaaccaa 21840
agagatagaa catggaacat ccatacgatt taaattgtta ttcagtgcct aatgaaatca 21900
tacgcttagg tgctttcgtg ctattatatg catactcacg tagattcata gatcaactac 21960
gtgatccacg tatcacctat aagaaagggt ggtatcagtt cagatatcat aagagtggta 22020
atccaatctg tattagtatt tcttgggacg gtacaaggta tagaaataca cagaagtatt 22080
accgtaagaa taggctacca aaacaagagg gactggtatg cgaactagat acattgtatg 22140
ggttaccgtg cttatctgct ccatcgctac agcagtagca tggggattta tactcgcgtc 22200
ataataagga atttgaacca tgttaaagat attcatcatc ggcttagtac tactattcat 22260
cttcacaaac ccatgggtac tgttgttccc attaaccgca ggattaatct gttttgttgt 22320
acaattattc tacatgtata tgggaaagta gaacttctta gaacttctca tccagacttc 22380
tactcatata ctaaattgaa ccaaatcact gaaccactac catatcggtg tcatactcta 22440
acagggttaa atggcacctt tatcatatcc taaaattgaa ccacaaatga tcactctcac 22500
cgacataatc ctagtcatat tgatatttgt ttacctagaa ggcttcgcct tctcttactg 22560
aattgaacca cttggagata gaaccatgaa aacattaatg cttattccac ttatcgctgt 22620
acttgctggc tgtaactcag gcgatggcaa cacttacgca agtgaaccaa aacaatgctc 22680
agactacgca actgtagtgt gggaagacaa ctcattcgaa gcattcccaa atacctgtaa 22740
tgacatcaaa gcaggaacat ttactgatgg cacactcact gagctatcaa ctgtaacaac 22800
cacattcgga tttgatacta tccagtcact ctacacagat ggtgaattcg actatgcagt 22860
tcgtactgat ctacgtgatc gcacactcaa tatctattgg agtgataaca agcaatgcta 22920
tggcccaaaa gacaacgatg taatcgtatg tgacttgagc tatgacttgg attactcaga 22980
cacactcact cgtgtaaacg atgtacttgg cctttaattg aaccagagaa taaccctatg 23040
actactcgcg tatttactcc attcttcaaa actgttggtc atgctgacca catgactact 23100
caagttatcc aagaagaaca aatgctattt ggtgctacca ttaatcacgt tttaactaac 23160
tttgataaat atcctattac ttttcatata cttaatagaa tacttatgtt ggatgacttc 23220
aaagagctga tcgttgatca agctatgctc ggctatcatc cagtcattga taccaaagta 23280
gttttactta tgcctggaat gtatccgtgt ataggtggtt ggcattgtga tggtgttatc 23340
cgtaaagacc agaactctca acctaatctc gataccctag atgaaccaat tcaacacttc 23400
atctgtacta tctcagatgc aggcgatgag cactgcggta cggatatcct tgcacgtaag 23460
gttatctacg acatcaatga tgatgagaat atttgggcac aagtgaacca gaaagtagaa 23520
atgactggtg acgtgcctat tcatactaca aagtcaggtg aaatcgtgca gtttgatcgt 23580
caaacgttac atcgtggtac agcagcaaca gtacgacaat ggcgttactt cttcagactg 23640
agcttctacc acatgccagc aatgaaccaa gatcgtaaac aagtgcaggt atactctgat 23700
atctcacaag gatggtgata tgagtagcag gctaaagaaa tttgagtaca acgctaaacg 23760
tacttatggc aataattcat tcagtatatc ggaacgtctc tgcttctgta tgggcctcaa 23820
gtatgacaat actactgtca gaggctcatt tcagctatgg aggaagcgta ttcaacctgc 23880
tactgggcta ccatcactgt caaacatagt tcagttgttc cacaaaaaac cttgggagtt 23940
aatagatgag cagactgcaa acgctttcag caaacagaaa tagagctgta ttgttccacc 24000
atgggacaca tagcgcattc atttgtgaat gtatgggtat tcatactaag gagacatttc 24060
gaacagtatt gaaaataatc cctgcaatcc gaaataagcc cagcttacta gctattttac 24120
gcaacgcgtt agaaaacagg ttatgggagg atatctatgt cgagactgag cacctatgaa 24180
gacaaactgt ccagatatga ttcaagctgt gtagcaattt gggtagatga aatatttcca 24240
aatagtgatg cttgggaaat tatagatgaa gtatctgatg cccagtgtga ggttaaagct 24300
tgcattgatt gcaactggca aaagcataac ttagcgaggt tattagatgt cctatctgaa 24360
ccaatggaat aaagaatcgt tgctgaaact aaaggaggtg atccgtgaac agcaggctaa 24420
gcaaagtgga aaagacttgg gcaaacctaa gagacagcac agaagcaaac ggctgtcttg 24480
aagcaatgag ggcatcagcc ggtatcagtt atgatgaagc acatcaattg aaccaaaagc 24540
tatgcccaac ggattacaat ccgggtatcc aatgccatac cgaatgtata tttagctgtg 24600
tcgagtacga tatagagatg atgagtaagg tgcttcgcac cttggcggtc gagtaatttt 24660
aagaataagg atttgaacca tgtcaattga acaagctaaa cagcgttttg ctaaattccg 24720
tgcgctagtt aagaaacaaa tgaagctaga acataaccat gctaagcaga acactacagc 24780
tatggtcaag gtgttcctta aaccgtttca tgctgacttt gtagaagcag cctctaaact 24840
agagcaaatg atcagagcca agaaacaatc tgattacgaa tatgtaacca aaggtggtga 24900
acaacgtgtt cgtgaagatc taaaagaagc acataatgta ctattagagt ctgatattgt 24960
ggatttgatg tatagaatcc actctgtagc atgtacaaag catgagatta ctctgcaaca 25020
ggcagtaggt agtattattg gtaacttctc agagattgag attgttacac gtaagttact 25080
agcttgtaac cttgtgcttg agtactgtcc tcacctaatc atcaacatca ctaaaggtgg 25140
tgagtatggt tatgtcggta gtctaattcg cttagacaaa gaagaggctg acctactgga 25200
tcaacactca gtagcactac catcaatcgt tccactacgc aaagtacgta acaatgctga 25260
tatcggttac cgtacattta agaagtctgc aatcatgggc ggtaagcacc atgacaaaga 25320
catctgtttg aaccacttga acaagcgtaa tgctgtaccg ttcaagatca acaaagaagt 25380
agctggtcgt atccttgcag gcaagttggg ccagttcgat cctgaaccaa agttcaataa 25440
acgtctaggt cgtatcgaga acgaagcaga agttcaagag cgtaaagacg catggcttga 25500
tcgccaaaag catatgaaga gtaaaatccg cacacttgca gaccatatat tctacttctc 25560
tcaccgtaga gataaccgtg gtcgtacata ctgtgaaggt taccacttct cataccagag 25620
cactgacgat caaaaggcaa tcatcaactt ggcgatcgaa gaacatgttg caccagattt 25680
ttagatggat acgccgtact tactacggct acccacactc acgtttacaa accttactag 25740
atgaacgagg cgatgcatgt catatcattg ccttaaacat ctttaaccga gcactatgtg 25800
cgaaccaaga aaccaagtac aagataactg gtgatattgg ttcagcagca gaagcctgct 25860
ttcattgtcc tatccaaaga gattatgaag gccataaatt agatattctc gtaagagaac 25920
ttgaatcaac taaataagga accaaaccat gtcaatgcaa cactttactc ctgcgcaatg 25980
ggcactaatc gacctagcaa ctcaatacgg taatgacaaa gatctgtatg acgatcgtct 26040
acgctttggt cgtgaaattc tcgagtccat taaagaaggc actgttgaac catggatcga 26100
agcagcagag aactcagcac tatttactaa aggtgtattc gctgtagaag agatcctaca 26160
aggcgtacca actagccatg acatcggctt agatgcagca gcatcaggcc cacagctact 26220
atctatccta cttcactgcc gtactggtat ggagaacaca ggtgcactta acactggtcg 26280
agtaccagac gtatacactc gtatctacga gaacactaca atccaaggtg taactcgtaa 26340
ggaagtaaaa gctgcggcag taccattcgc atatggttca gatgctaaac ctctacaagt 26400
attcggtgag gagaaagtag aagaattctg taaagcatat gagcgtacat tgcctgaagc 26460
atactgggca cgtcagactc tgatcaatgc gtggaaccca gaagcgctat tccatgagat 26520
cactctacct gatggttatg tagcacatat ggaatcacgt ggcctagaag aattccgtag 26580
tgagatcttc ggctaccgtt acacatacca aactactgtt aaccgtccac tgaagaaagg 26640
tgagaacaag actaaagctc tagcagctaa cgtaactcat ggctatgatg gctttgtggt 26700
tcgtgaacta gataaccgtt gtaactacaa ccaaaacatt gttcgtttcg ctatcgaagc 26760
aatcgacaac catttggagc acggttcaga gtttgaatat gaaccattcc tgcgtattca 26820
aggtcttgct ctacgcagta actttatctc tgtagctaac ctagagaaca ttcgcatgaa 26880
tggcctagaa ggtgcagacg agtcgtactt gctacgtcta cgtgagcgtg ctgtagatat 26940
gcttatgaat gaatcattcg taacttactc tattcatgat gagttcaagt gtggtttgaa 27000
ccatgttgaa cgactacgtg aagtctacaa ccaaatcatc gctgaatctt acttgtctac 27060
ttggctccta gatgtaatcg aagacctaac aggtgatcgt ttcgaatggg atgaaccaac 27120
agacatggaa atctacaacg aattgctaca gtccaactat gcaatcggtt aatacacagg 27180
ctaccttcgg gtagccttta ttttttgagg ttagtatgag cagactacaa cggatagata 27240
aattaattaa tagccctgcg aaccagattt atgagggaag tgtgcgaacc agaatgaggc 27300
attgtccaca tcaggcagag ctaatgggca gtaaaaccaa aaggtgtatc atgcttgata 27360
aatccgatat gccgtgctca ggatgtccag tagatgaaac aaaagtgttt aacgatttaa 27420
tggaggatat cttcgatgag taaattcgat acatggttag aagcagcacg tctagataaa 27480
ggattacatt tcgctgatca aagttgcttt aaccttaaaa ttgtaactag atccgatttc 27540
aatgagctat gcgccaagtc tgcaccatgc gctacatgtg ccctgtgtaa caacgtaggc 27600
atggattaca tagccttgtt agatttattg gggatacagg atgagtaggc taatcacttt 27660
actagaattc gagaaagtac cttatagctt gaagactgga tttttgtgta ataacccaca 27720
aggtgcagga gatttaaata taccttgtgc aaaagtatta gaaaagacag atatagcaat 27780
aagctgtcgt aactgtccct tagataataa agaaaaattg gttcatttct ggagaaagat 27840
agatgagcag attaaataag atgatcgaac acaataagaa ccaaggaaac atgcctaatg 27900
atccgggaag gttcaacaca tgtgcaggcc atgctaaaaa gttaattgca aattacaagg 27960
atgatgggct ttgcccattg tacggggcta tagacagccc aaattgctta aagtgtcctg 28020
cggattcttc taataaattt ttaagactaa tggtggaatt atgtcccgac tatcaagatt 28080
agtattcctt agaaagaaat atacccgttg gaatgatgca ctttgtaagg ctatgaaagt 28140
acctaatata agatttgcct gtcctaagtt tttgaaccat ttagaggaat ataacgaagt 28200
atctagaata tctctagctg tagctttaga tgatatgctc cttaaaaatg gaggaacact 28260
cgatgagtcg cttaaaaaga ttaatcgaaa tcatattaaa agaggatgaa aacagaactt 28320
actcttgtga agaacactat actggttcag atcatatagt aatttgctta catccaaaat 28380
acataacaac gttagaattt cttgaatgcg ataattgtcc catacaggag atctattttc 28440
taaataaagt attggagatt ctggatgagc agattaagca aactaattaa acagttaaaa 28500
aagaatacct tagatggcga atacccttgt gagctagtct cctacagaat aactgaagac 28560
cgatcggtta caggtacagt ctgtaatgct ccacatcgtc tgactgaggg agaaatatta 28620
tctaacgatg ccttggttag aattaaaaag gatgcctttg taccatcaaa ttgtggtatg 28680
tgtgctttag acagcaatca aatattcaac gaaaccctaa aactacttca ataaggaata 28740
acccatgtca cgcttaaaaa ctttaagaga tgaaaatgct cgcaaacgat atgaaccaaa 28800
cttctgtcgt tatactcgtg gttgtgatcc tgcattaaga ggaatgaacc aagcatgtcg 28860
aacatgtccc cacggaagaa ataacctacc taaactttgg cataaattaa gtggggtaaa 28920
tgatgagtag actcgatgta ttaaaacgca aaaagctttt acttggtgaa tatgcactag 28980
ctatccattg taaattcaaa ggctcatctt gtcctgaaca attaatggat agggcttgtg 29040
tgcattgttt tgcgaataga gacaacttac cggatatgat gaatagacta gatgaggtga 29100
tccatgagcc gaattaatac gctactcact aaacgaattg gtcattataa atattccaac 29160
agtggtgcct gttatgtaga gctagatgct caagaaggac accgtaatct ctgtaccatt 29220
cccggaacca ttacaagttg tagcagatgc ccatggcaac accggtctat cgctaatgaa 29280
ttccttcgga aagtgtttgg caaagacact ccactgctta cttatgaatt gagtgagcgt 29340
gaatcagacg agatctcata ttatgagcaa gctagacaag ctgaattcga cattgaacca 29400
acttgaggaa ttatccttta ttgatcctga tgttccagat gaatatgcaa gctgtgtatt 29460
tgataataca ttgggcttaa ctgacttccc ttattctgtt tgtcctaaag aaatagcatt 29520
gggtgtagaa atggatctaa ataactgtaa ctcatgccct gcatgttcac ctaaacgatt 29580
caacctctta gatagcgaac ttcgttcgct tttatgaaat taaccttaaa taggaaacct 29640
tattatgtca gatacaaaga aagaactacc tgattacttg aagaattcac agatttactc 29700
tctgtacctt attcttgaag caatggaacc agaaccagat gacgctgatg ataagcgtat 29760
gcagctaatc ttctctctag tagaagacgc tgaattgctt cagtgctcac ctgaagaagt 29820
actacaaggc cgtaaggctg cagctaatcc agtaggcgat atccctgttg cagtaaacac 29880
tacccacaac ccgtaattga accaaaggag ttcctcatgt ctcgtaatga agctttcgca 29940
attgtcggta taatgctagt cgtatttggt tcatttatcc tattttccaa aataggacga 30000
gagattgatt tggcaaaagc cgaattaaag ttcaaacgtg ataaagcaca tcaagaacta 30060
gtggttcaaa agattcgtga ggagcgtctg ttactcgaag caaagaacaa acagttagag 30120
cttactggtt cattcattac caaagaaggt attgtgtatg atcagcagat tgacccggtt 30180
aactgaagaa atagttgatg gtgatgttag gtataataga tctatcgttt ataaagccat 30240
taatattatg acgggtaaac ctaatgttgg tgcctgccct cacactatgt tacagactgg 30300
tttaaactgt aacggctgtt cctttacctc tgtagataaa ttcggtcaac tcttagggac 30360
attacactta tgagtaggct tcaaatttta gaaaaagaat atggtctttt aactcaacga 30420
caaataaatc gtgctcagtt atacaccaaa ttaaataagc ttaaaaaaga aggttgtctt 30480
ctacattttg taggatatcc ccaaatacct tgtcgtgaat gtattttaga cagctattcc 30540
caattcaaaa aactcttaat ttctgtagat gccgctttca gcggctctta tgaataatca 30600
accaatctca atggagaata aaatgtattt aggtcaagta gcactagtag gtgcatcagt 30660
aggcgttcct gtaggtaact gggcattagt ttacggcaca atcgtagaga tctgcccaag 30720
cgtatgccac gctgttatcc gtgtagacga tgaggaagtt gtcgttgaaa aaggtaaagt 30780
ccaactagtt gatgtgaacc tagctggttc aacactaatc gacatggacg aaagcaagcg 30840
tgcagtattc cacacattgt gggctaacta ctggattgcg ctacaagtca aagttgacgg 30900
tgtatgggaa gacattcctg aaggtatgcg tcaagttcca ttctctgaac tacaagacga 30960
tgctgaacta cgcgttaagc caagcgacga aatgcttaaa gtgaagcgtg aagaaactct 31020
acgtaagatt cacgagctaa agcaacaacg tgcagcaact aaactaatga ttgaaaagct 31080
tgttgaagcg tctaataaat ctattgacga agaacaaaaa cttcaagcta gtctagctga 31140
catcagctaa taaaccccca ctgaaccagt tagaggtatt ccattaaccg taatgcgata 31200
cgtgtaagct ctgactggtt caatcctttc gagagataga ccaatgaaaa ttcatgaacc 31260
aaactataag attacccgta cccgagatga cgatggtgaa atggtatatt ttgtacatcg 31320
ccctaatcag cgtaaagtga tgatcattaa agctggcaga cttttcaagc ctgtctcacg 31380
taaacaccct tactaccgca cactgcgtga tgcgatcatg ggagtagtga accaatgaga 31440
ttccaatgca ctgaatggtt agacggtttt accgttggtt cggtgtatca cgctgtatat 31500
caattcggta aagccgaata cagaattaat aacgatgaag gtgtcgtaga aacactaact 31560
tcacaagacg ttgctctaca ctttgaagag tgctaatgtc gaatttttgc ccgagagact 31620
cgtatgctaa atgatcgtcc tatttctgaa atcgcagaaa tcatcttcaa catggatgat 31680
tcccgtgtac agcgtgctgc tgaactaaag tctcaaggta tccttgagtt atccaaagta 31740
gaagtacgta aacgtattca agaccgtaaa ttccaacagg aattgaacca aattgagagt 31800
ggagaattgc tgcttaatca tcccattatg gataaaaagt acttcaccct gtaccgagac 31860
taagatattg tatccttgag ttgtttaact aaggaacgtc ttatgtctaa atcaaaacgc 31920
cgcgaacaag aacgtaaacc tactcgatct gagaaagctt tccgtgacta cactccggct 31980
aaacccttac agcctctgaa ccaagcccaa gctgaatata ttcatgctgt cgaatcccat 32040
ccttatatca tggctactgg ctatgcaggc acatccaaaa cctatgtacc cactcgtatt 32100
gcagccaaat ggctaaagca ggaagctatc gacaagattg ttattgtccg tcctgccgct 32160
tcgatgagtg agtcattagg cttcttcaaa ggagatcaca tccagaagat gaaaatgtgg 32220
attgccccag tacttgatac tctcaaagag gagtttaaac cgggttatct cgactatctt 32280
ctatctgaag aaattggtgc tatcgactgt gttccactag agactgctaa aggacgctct 32340
tttaaaaatg cattcatcat cattgatgaa gcagaagacc taacaattga ggaagtgaag 32400
accttgctca cccgattagg cactaattcc actatggtat ttgctggcga cattaagcaa 32460
gtagatatcg ctaaatctgg cttaggtgag ttcttagaat tacgtaagcg cagtgaacgt 32520
ttacagcgtg tagttgatca tatcgacttt gacgactacg acgacattgt tcgtagtggt 32580
gccgttaagg aagtaatcat tggacttgat gaagtagggg cataagccct atactggttc 32640
agtctattaa ccctaattag gagaatataa tggttacgcc aatccctgta gacaaagccg 32700
tgtttgacct tattcagaca cgtaagcaag aaatcaacaa gtatcatgaa gactatttga 32760
aagctctccg tgaacttgaa gtaacagcca ctgtaaccgg tggtactgta gtaggtgatg 32820
tgccagccgc accaccatca ctataaaatt gaaccaataa tacaaagctc cttcgggagc 32880
ttttttcgtt tctggagtaa acaaaatgac caaatttcgt cgtaatgatc gagtgatcgt 32940
aagtaaccct tccttcatct gctacaagcg tgttggtaat atcgaagagt ttaattcaaa 33000
cggtacacct cttgtacgct ttgaagatga caattcatca atgcatatcc gtactgaaaa 33060
ccttgactac ttcaatggtg gttcaaatga taagtacctg ctgtggcaac ctgagtctaa 33120
ccgtccacca caagtaactt atggttcata cggtcaagcc cttaaagtgg ctaaagacat 33180
ggcccaacgt caccgtaaga agttcattat ctgtaaactt gatactgaag tagaactatc 33240
cgactttgta ataaaaaagt tgtaagcgag gaaccagatg tcaaaactac aatggcacgt 33300
tcgcctatgg tcatcaatag cgatgctgat tctgatcgtt tatctatccg taaacgcttt 33360
gctaaattac tcgatagatg gctacacgat aattcgaagc attgatttag catttatcac 33420
tatcatggct atgttgaaca tcattgttga tgacatctgg cccatggctg acaaatatcc 33480
aaagagtgaa ccaaaaaatg aataagcgag tcgtttatga gaacgccact gctaatccta 33540
aagtggtctt ttacacggtg ggagactcaa cggatgagtg taatcagagg gttgaaccaa 33600
aaggaaagat caagctacgc acagatctgg ccttcaacct aatcaaaacg gagactgtag 33660
actaatgcct tacgttatcc tacttactgc tatttggttc attgccgcat gggtgaacca 33720
cctacttgtc tgcttcaatg aagaagcatg gggattcttg attgctggtg caatcttctt 33780
cccaatcggt gttgcccacg gcacttggtc gtggttctaa cgtagaggcg ttccgcctct 33840
ttcatgaatt tgaaccaaac taaaggaatt ccaaagtgaa tctaagcaaa gcaaacccat 33900
ttaaaattgg tgcaatcgct atcagtagtg ttgtagcact atggttagca ctgaacgtgt 33960
ggactatcgt accggctggt cacgtaaaag tacctgtctt attcggtaaa gtttccgacg 34020
atgtacttga agaaggtttt aacattcctg ttaacccatt cacgtcattc gatacaatca 34080
gcattcgtac tgataaatac gaaatcaccg gcctgaatat cccaacacaa gaccgtttca 34140
actctactgg taacgttaca gttatctacg atattaacgc agataaagcg agctacattc 34200
gtaaagagtt cggtaacatt gatcaattta ttgataaaac tttacgccag caactacgtt 34260
caattatccg tagtgaaggc cgtaaacttg ctgactctcg tgcattggcc caagacgtta 34320
acgtatccaa gatgcagatg aatactgcta cacgtttacg tgatgcactt gaaactgccg 34380
gtatcaatat tcaagaagtg ctggttcaag atattacatt tgatcctcgt attgctaagc 34440
aaattctaga tactcaaaaa cgtatccaga acgaagaaca aaagaaatct gatgagcgta 34500
tcgctaaaac tgaagcagct attaagaaag aacaagctat cggtgaaggt aacagaaagc 34560
gtgaagcagc agatgctgaa gcttaccgtg aaaagacaga agcagcaggc cgtaaagaag 34620
ccgctatttc tcgtgcgcaa ggtaaagcag agtctattac tctacaagct aaagcagaag 34680
cagatgctat taaactaatg gctgaagcaa accgtgactt ggctagatca atcacacctg 34740
cactacttga atacaaacga atcgagaatg aagcaattct ttactcgaaa tctcgtggtg 34800
aagtaccaac aactattatt ggtgatactg atctacgtgc aatcggtgtt cctgtagcaa 34860
ccggcactaa ataattgaac cagtccctgc caaatggtgg ggactctttc tttggattat 34920
tcgataaggg aggacttcgt cctcttttat gaaataacct caagaaaact actaaaggag 34980
tgaaccatgt ttcacaattt ccaagaagct gtaatgaaac aaatcgaaaa gatgtctcgc 35040
tttccactat ttgaagtgga ttactctcgt gaggagatct ttgaaaagta ccttaactct 35100
ttccgtccag aagataaccc aatcttccgt gagcgtactg aacatgattg ttcatgctgt 35160
aaatcgttca tccgtcaaat gggtggtatg gtcgctatcg acaaagataa agtgatcaca 35220
atctgggacg ttaaaggtct tgaaccaaaa taccaaatcg ttgctgatgc aatggctctg 35280
tatatccgtg ccctgcctat caaacacgtg ttcaaacgtg ctgaagcaaa aattggcaca 35340
aagcaaaacg tagaggtaac tgaaaatggt tcaattactt gggatcattt ctattctgaa 35400
gtacctatgt ctgcccgttc tcgtgatggt gatcgcttgc gtggcgaatc cgcttctcat 35460
aaagatagtc tggaacgttc tattacagaa ctaaacccag atcatgtgga agaattggtt 35520
cagttgtgta aagacaacaa cctgtactta ggtagccaac aacttcctaa actacagaaa 35580
cttcaatctc tgcaacgcga atacaatgca gctaagaaca aggagctatt cctatgggta 35640
cgctctgtgg agctgggcgc tgcgtctact attcgtaact ctgttatcgg tcaggttcta 35700
gtgaacatgg ctaaaggtga cagcctagag actgctgtag gtaaatatga ggctatgaca 35760
gccccatcta actacaaacg ctctaaagct atcgtatcgg aaggtatgat caagaaagct 35820
caccagacag tcgtacagct tggtctggaa gactctctac ttcgtcgtca tgctactaaa 35880
gacgacatca caatcaataa cgtactttac gctgataact ctatccgtga atcgcttggt 35940
gcattcgata tgttggttgg ttcaacgagc cagtctacag aaatcaaagg cactccaacg 36000
cctatgggta ttgagcagtt cttagaagaa gtactaccta actcaagcaa gcttgaagtc 36060
tacatggaga acagccactc agctaaccta atgagcctag tagctccggt taacgctgat 36120
gcgcctaaca tccttaaatg ggacaacaac ttctcttgga cttacaacgg cgaagtaacc 36180
gactctatct cagagcgtgt taaacgtgcc ggtggtaagg taacaggtaa attgcgtgtg 36240
tctctggcat ggcataacag tgatgacttg gacttaggtc taactgaacc aacaggcgaa 36300
catatttact tcggtcactc acgctcacgt caaggcggtc gtcttgatgt agacatgaat 36360
gccggtggac gtatgaaccc tgtcgatcca gtggaaaaca ttatctacgc agactctgtg 36420
aaagatggtg tctaccaagt caaagtgaac caattctcta aacgcaacaa taacaacgac 36480
aactgtggtt acacaattga agttgagtac gaaggtaaga cctacacgtt tgagtctgcg 36540
gctcaaatgc accaaggtca gacagaccta gtgactgagt ttgaagttaa gggtaacgag 36600
atttctttct ctggttcacc aagctctgct tcgaaagaag tatggggcgt cgctactaag 36660
cagttcacta ctgttaaagc agtatcacta tcacctaact tttgggatga gaaccaaagc 36720
ggtaacaagc actatttctt cttcttagat aattgtgtta acccagaatc agctcgtggc 36780
ttctacaacg aattcctacg tgatgacttg tacgatcacc gtaaagtatt tgaacatctt 36840
ggttcaaaac tgaaagctga atactccaac gatcagatgt ctggtcttgg cttctcatca 36900
accaagcgca acaaacttgt aatgcgcgtc gataaccgtt tatacgaggt acagttctaa 36960
tggaactatt cgcaaaagca acaaaacaac gtttacgttt taaaattggc aaaggtacta 37020
tctctacaga agacctttgg ttcattggca tggtcgagct agataaacta gccgttgctc 37080
tacacaaaga gttggaatct caggaaactg gttcattcct aaatcctaag aagactgacg 37140
ctaaaactca attagagttt gatgtagtta agtacgtact agaccaacgt ctagaaggtg 37200
ctcagcgtgc tgagaaagca gccgttactc gtgctaagaa cgaacgcatc gacaagatca 37260
tccagcgtaa acgcgatgaa gaacttgaaa acatgtctgt tgaagagttg gaaaaactca 37320
aagcagaata atccctaacg agagaggcct acggcctctt ttatgatttg aaccaaaagg 37380
aaactacaat gtttggtcta tttaagaaaa aagctgctgc tgcagcagtc actctacata 37440
aagtagaaaa caaagatcta atggaagcta tcgtagctgg tgctgtactg gttgctaacg 37500
ctgacggaga agcttcggct gaagaactaa ccaagctatc aggcatcatt gagaacaatg 37560
acaacttgaa gcactttggt tcagctatcg gtcagaccat cgacaagtac atgggtatgt 37620
acgagtctgg tcgtcgccta gctaagtcta agctgatgaa agaaatcgct gatgttcagg 37680
cagatgaaga tcagaaagtt gaagcattca tcattgctat cgagatcgct gatgcagacg 37740
gtgagattga tgatgcagag atggctgttc tgcgcgagat tggtaaagtc ttgggcctta 37800
acccagacaa ctacatctaa tgcaggagcg tttactcgcc ctattccttg gactgctatg 37860
tttactagta gtcatcgtag atttcacgag caagatcctg tctatcacgg tagacggggt 37920
tctcgtttgc ggaatcgtcc tcttgatctt tcgtctgcgt aaacttgatc aagaaccaaa 37980
ataatccaat aattcaaagg agagactcat gtctcaagaa caagcaatca cagtaacatc 38040
aaaagcaaca gcatctctag acaaagtggc aacaagcgta gcgaaagcag tagctgacct 38100
acaatctgta actgaagtac acggtgacct tctaggtcaa gtggctgaag cgcaacagaa 38160
cttgaaccaa atcgaagaag atactgcaac taaagtacgt aaagcaaaag ttgaacttga 38220
ccttcgtgta actgagcacg ctgatggtgt tctacagaac cttctagctg agcgtggcct 38280
aacttctatc gacgaagatg cgctagttga gctacgtcat aaagctgatc gcacttcata 38340
cgaagttgat gttgaaatca agaaagcagt taatgcagca cttgcgaaga aagacactga 38400
gcacaaactt gaacaagcag aaactaacca agttaatgca gtacgtgaag ctcaaatgga 38460
atctcgtatc gagtctctag agcaacaact tgaagcatct cgtcgtgaag ttagcaacct 38520
aactgaacaa ctatcagcag aacgtgaagc tggtacagag cgtgcaaaag cagcaggttc 38580
aatgactatc aatgccggtg gtaaataata gtcgccatgg tagggggagc tacgctccct 38640
tttccgaacc ggagactcgg atgagattcg gtatataaaa cggggttgaa ccaacctagg 38700
gcagatgcaa aaggctttgt caggccagct tacatcgttc agtcggagga ctgaattgaa 38760
ccaataaact aagcgaaggg ggacgccaac cccacgacac taactactat tcgatattct 38820
ttctttatgg gacattccag tggcttaaat gcagcatgga attgacaggg catagctagt 38880
cgtagagggc tgatcacctc gtagacacat taccccgacc gtgtagcaat actagttcaa 38940
gcagacgata aaaacagaga gagaataatg aatagccgtt gacatggata ttctgaaata 39000
tgttactcat attttattcc ttatgaagca gcacttcctt gtgctcggca agtacatgct 39060
agaaattggt gtacagggta ggtgagatgc ctaccgtcat tctgaatgtg ttttgaacca 39120
gaatacatcc agaatggcat tgctgttcag tgacattttc taatgtctcc aaagttggaa 39180
tatatctatt tacctcatag tagatcggcg agagtcgcta gaaactggac ttcgattcag 39240
gtgggatgcc tgatacctat tcactacaac acgtcctgat agtctgtcgt tgtagcctgc 39300
cttcgggcag tcggcaagcc gatgctagaa attgatcgga tgagtgagtg agagacttgc 39360
acctaattta actactggaa tttgaaccat gaaaaaagta acccctgaag tggctgaatc 39420
attgaaagct attactggtt gcccaagaag aactgaagac atcgttaaaa acttcgacat 39480
tgatcgtatt tcatttttga atcatattcg atctgctcgt gcttcgggta ttccagtaga 39540
acatattggg ccaatgtccc atcgttattt ctatctccca gccgaatacc gctataacat 39600
caaagaaaca gttgcagcat caattaaaaa gatcatggcg aatgctcggg aagaggatga 39660
cataactgtg gaatacctcg cacaagtatt ggaagtaacc actaatcatg tacgttcagt 39720
attctatgcg tggaagaaga aaggccaagt aatttacagt gcacctactg gtgttggtaa 39780
acaaaagaaa tactggacag tgcctaccga acctgttgaa cgagagtctt gactctggtt 39840
catcgcaaac tctgcgatta ataagtgatg agtgaagtgc actgagagag tgcacaactt 39900
taattaactt agaggaatag gcatggcaca agatacctta gctaaaaaga agcaagcgta 39960
tatcctgttc aaacaaggtt ggactgtgaa gcaagtagcg cagcatatga acgtaagtac 40020
tcgtatggtt taccgctaca aagacgaata tgaattacat actctccgtg agttccgtga 40080
tcaattcaaa gttgctctgg aaatcgcaga caaacaacaa cctttatctg tatttttgca 40140
gatcttaaaa gcacaagcta ataaggaaaa gaacagtgaa aaatccgctt aaatttaaaa 40200
cagtagatga tgtaatgtcg gatactgtcg gtcaactaca agagatcgaa gaacgccaac 40260
agcgaaagtc tgaagcgtta gaaaagcaaa ttgaaaagtt gaaccaacag aaggaaactt 40320
ctgacggtga agttagtaag gccaaaatgg gcattcgaaa ctttaccaaa ctatttactc 40380
aggaattgcc cgacaacgaa gagtaatgac aaaacctaag tctcctgttt aaggggactt 40440
tatttttgtt attgctaacc gcacctctat gctgaaggtg gaaaggttac agttggctgg 40500
gcaactgaga catgcaagtg aaccacctta gtagttttct tttacttgcg tgaccccagc 40560
attattcccg aataaggcgg cttatccttt aatcgtatgc taacacgata tatgccgctg 40620
aagtccccgt ccaagagtag taatcttgcg gggcaaccta tttacacaaa cccccctatg 40680
atctgaatac gccgggttaa tttgttttgc cactgcgtta actaacgaac acctaaacat 40740
gaaaactgag gtgatcgtct atggtcttta ttcagtaggg gttttttatt attgaggcag 40800
gaacatgtct aaagaacttg aattcgattt atttcgtacc tccaaggaaa cacaatttcc 40860
tgctttagta gatttgcagg tactagaccc taattcagca gaaaagctaa tcaacaattg 40920
gttcaatact tcccagtcaa atgaactcct agcgaaagaa atacgtcaaa atcgtatgac 40980
gatgagtcat ttgaaccaag ttattgaaga acagaagcga gctatcaaga aagaaggtat 41040
agctggctta gtgatcggag ccttaatggg tgccgccact gtttctttac tagttatgat 41100
ctttggggga tagaatgcga tcgtttaatc aaaaagagat tgaagacttt ttgaaccaat 41160
ctcctagtaa ggcatacaag ctagtgcctc accaatttaa ttctagtcat gcaggcaaag 41220
cttgctgtac taagtgtggg ctgctcgccc ttcgaaatag agctaccgac tggtgtgttg 41280
aaaaaggatg tgagtttgag aaccaccctg attacaccaa tgcaatgaaa cgtttaacca 41340
aataaccttg agagaacaca atgagtaatt tagattttgc agtaactcca ttgaaagcaa 41400
tcccattgat tgaacgtgca atgcgcaaac gtattgtacc aatcctgaag ggttcaccag 41460
gtatcggtaa atccgatatc atgcgtaaga tcgcacgtaa gcacggtctg aagctagtag 41520
atattcgtct agcacaggtt gacccagtag agcttaatgg cttccctaac ttcactgcgg 41580
acggtaaagc ggtttacgct ccaatggata tcttcccaac taagggacaa gagctaccac 41640
ttctacctga attcgaagac aagaaaggtg aatatgatgc cgtacttggt tttggttcag 41700
aagaagagat caaagaattc cagaaagagt actgttacaa aggttggctt ctattcttcg 41760
atgagattac ttctgctaac cgtcaggtgc aggcagcagc atacaaaatc atcctagacc 41820
acttggtagg ccaattcgat ctacacccta atgcactttg tgtagcagct ggtaacctgt 41880
cttcggataa agctgttgta aacccaatgt ctacagcact tcaatcacgt atgtctcact 41940
tgactctacg tgttgataaa gacgaatggg ttcattgggc aatcgagaac aacgtggatg 42000
ctcgtgttat tgcattcatc gaataccgtg atgatctgct tcacgacttc aatccgaacc 42060
atacctcaga taccttccct tgtcctcgta catggaaatt tatgtctgac cttgtggaag 42120
acgatgaagc aatctctagt tcactaatgc cactattggt tggtactgtg ggcggtggcg 42180
cagcaggcga gttccgtacc tttacagaaa tctggcgaga attgcctagc cttaaagata 42240
tttcagtaag ccctgagcgt actacagtac ctaaagaagg cgctacctgt tacgcaacag 42300
taggtatgtt ggcatcatct gttgacctaa catcaattga accaatctgt aaatacgttc 42360
gtcgtttggg ttcagagttc caactagtgt ttgttcgtat ggcgttcaaa tacaaccgta 42420
ctctaatcca aaacccaaca ttccagcaaa tgttgaaaga cttccgtaag gacttaaaag 42480
acgaggaata aaatgtttga tcgtgataag gctctaagta aagctaaagt tgccattatg 42540
atgaaaccga atactgcatt catttcatct gtattaatgt cacttaaatc taagtgggat 42600
gataaacatg ctacggctta tacagacggc ctaagcatcg gcatttctac tcctttctgg 42660
gaaagactaa caccggggca acgagtattc ttgttgctcc atgaaacttg gcatgtggcg 42720
ttttaccatg taactcgctg caaagaatcc aagaaatatg aaccacagaa atggaattat 42780
gcctgtgact actacatcaa cttgatgttg gtcaaagaag ggtttgaatt catcgaaggt 42840
ggtttattgg actacgccta tgatggtatg tctgtagatg aaatctatga tgcattacct 42900
gagaacctac ctaacaacct gttagatggc gatatggtag aaggtggttc aatggaagaa 42960
gaacagccta cacctgatga cgttaaagca cgtatcactg aaactctatc aagagcaatg 43020
atgcaagcaa acatgtcagg ccaagctggt tcaattcctt tggatattcg acgtgcagtt 43080
gaagagatcc gtaatccacg attaccttgg aatgttgttc ttgagcaata tcttagtgat 43140
cgtatccaag atgaatattc ttggaacaaa cgcaatcgtc gtttctccca cgtgtatctt 43200
ccggcgttag actcagaagg tatgggtgag attcgttgtt atgtggattc ctctggttca 43260
gtgagccaga aagaactatc tcttgaagta aacgaaatgt gttacatcaa agaggtatcg 43320
aatcctacat taatgacact tcgtgccttt gcgtgttctc tgggtaaagt acagcagttt 43380
gaacgagatc aagaaatcga gttcgatgtt gacgtaggtg gtggtacaag tttcggccct 43440
atctatgacg acctgatgga caacccagat actgaagtgg ctgtaatctt tacagacggg 43500
tgcctaaacg tacctgacac atcggccctt ccatgtgacc ttgtatgggt tatcgtgaat 43560
aaccctactt ggacgactaa cacaggccgt gttattcaca tggagataaa acaaggagac 43620
tagaaatggc acgtaatccg cgtaaacgta gtgtgtcttt tcgaaactac gccctgcatc 43680
atactaagtt atgttgcttt tccaaagttg aaccaaagca gctagatctc gtggtgaaac 43740
atggcgcttg ttcatacaaa ggattagact ttgcgactcc ttggcattac ggcgtgattc 43800
caccctgtga tgaagcaaag tgggggcgtg atcttgaaca acttgatcac atctggttca 43860
taactagcga atggaagaag attgaatttc atgtcattca atggttgaac cactgcaata 43920
gtatggtaga catcgttaag ttgttgccag actcaattac cgataagttt gatacaccta 43980
ttactggtga gttaaccctg tcggacgagt ggatagacat ggttcaaaac tctgatgagt 44040
ttaatatgtt agaaacactt gtgttcacta acgagatctt ggagcaatga tgtgccatac 44100
tatttggcct tcatcaaaca gaggtctata tgcgccatct tattcattct gaaaactacg 44160
atatggcagt actcattaaa gatgctgccc ttcgtaactc tgaaattgaa aaacactatt 44220
tgaaccaaaa aggattcaaa gctatctcct tctcacttga ttatggtgga aagaagaagc 44280
cgaccgctac agtggtgaaa gattatcttg atacgttgct tccagtcctt gagcaatgtg 44340
gagtacgaga tctgcttgta tgcgatacgg aatatttcaa aaagctttgt aaggttcaaa 44400
aagctgatcc tcattatggt tacattctgc cagtagctat tcccggctac gagcacatga 44460
atgctgtctt atgtaccaac tatcaagctt tgctattcaa cccgaatatg caagacaaga 44520
tagacctagc tctcggaacc atggaacagc acaaagctgg ttcatacgaa gagattggtt 44580
caaacattat tcagcatgaa gagtacccag atcagtatga tcaaattcag aaatggctcg 44640
atagcctatt acttttgcca gcccttacat gcgatattga ggcttttagt ctcaagcatt 44700
atgacgctgg tattgggagt atttcttttg cttgggacac tagtagcggt attagttttg 44760
ctgttgacta tgtgccaaca ataccccatg aaattaaagt gtggtgtaag aaagacaaga 44820
aatataaatc ccgacaagcg gtaggcaagc agggatacaa tatgcgtatt cgccaaatgc 44880
tcaaagagtt ctttatcaag tatcaaggct ctctgatctg gcacaacgcg tcttacgata 44940
tctcagtgct tatttaccaa ctctggatgg acaacatcat tgatcaagaa ggtatgcttg 45000
aaggacttga agttatgaca cgtaactttg aggacactaa gatcattact tacttggcaa 45060
ccaatagctg tgcaggcaac aagttaggtc tgaaagacca agctcatgag tttgcaggca 45120
actatgccga ggacgacatc aacgatattc gtcttatccc taaagatcag ttgttacgct 45180
acaacctgat tgactgtcta tgtacttggt acgtcatgga caaacactac gacaccatgg 45240
ttctagacga acagtacaat ctgtacgttg acctaatgaa gcctcatttg attgatatca 45300
ttcagatgca gctgaccggt atgtgtttgg atatgaacca agtccataaa gctgagaaag 45360
agttgcaggg tatctacgat actcacttaa atgcgttgat gggccaccca atcatcaaag 45420
agttcactca ccagcttcgc cttgataaga tcgaggcaga caacaagaag ctcaaaagca 45480
aggagcgatc ctatgaagag gtatctcacc ttacctttaa tcctaatagt ggtgtgcagt 45540
tacaacggtt gctctacgag tgcatggagc ttccagaaat tgactacacc aagtcaggtg 45600
ctccagccac cggaggcaag accttaaaga aactctctaa gatgacacag gataaagacg 45660
ctatctttat tttgaaccac ttgagagaat acattggcgt gaataaggta cttacttcgt 45720
tcattcctac atttaaggaa gcacccctag caccagacgg aatgcattac ctatttggtt 45780
cattcattct aggcggtact gtatcgggac gcttatcctc caaaaaccct aaccttcaac 45840
agattccatc tggttcaacc tatgccaaag taattaagaa atgctttgtg gcacctaaag 45900
gttggctatt ctgtggagca gactttaatg ctctagaaga taggattaat actctcttaa 45960
ctcgtgatcc aaacaaagaa aaagttctgc tagatggatt tgacggacac tcgttccgta 46020
cttatcactt ctggcctgaa aagtttcctc acattgatcc tgaagaccct gttagcgtga 46080
acaccattca gaaagactat gatgcagatc gtggtcgatc taaaccggtt cactttgcta 46140
tgcagtacca aggcacttgg gcaacactca tgaagaactg tggctttagt gagcaagaat 46200
ccaaggatat cgaagctaac tatcaagttc tttataagaa gtctttcgat tgggtacatg 46260
cccgaaccaa agaagcttca gacaaaggat acgctactgc tgctttcggc ctacgtatca 46320
gatgcccatt gctaggtaag accatattag gttcaagggc tactttgaac caagcggcag 46380
cagaagcaag aacacttggt aatgctatct caggacaatc ctacggattg ttaaatggtc 46440
gagcatcagt tgaacttatg cagaaagtgc gtaatcatcc taagatgaga acgtgcatta 46500
agcaatgtgc gcatattcat gatgcacagt acttctatgt gaaggatgat ctcgaagcaa 46560
tcgaatggtt gaatcgtgag ctaccgaaag ctatgagatg gcaagaactt ccagaattac 46620
aacatgaccg tattacacta ccggctgaat tagatatctt ctaccctact tgggcagacg 46680
gtttccagct agagaatgat atgactcagg atgaaattgc tgaagtttgt cgagcagagg 46740
ctcaaaaacg aagagagagt gaaaatgggt aaaacctaca aagatcgggc taaccacgcc 46800
ctacatcatg gttatgaacc aaatggtgat aaagagcaag agcttgctga tatcttagac 46860
cgtaatcctc acaagggtct acgccacggc aacaatcgta agagtgaagc caaatctaag 46920
aagatcgaac gccgcaaact tcgtcgccaa aaggagaatt tcaaccatga ttaaaatcaa 46980
taacaagcat caaaatgcta cgttacctca aactgtcgaa aacattgcac gctggcataa 47040
cgagcgtaac ctgatcgacg gaaccacttc gtataagcaa actgaaaagt tgcttgaaga 47100
attcaccgaa ctagtttcag ctcaattggc ccgagacacc ggtaagaaac cttcatctga 47160
attcgtttat gacgaaactc gctcaatgtt agaccgcctt tacgaaggtg gacgaatcaa 47220
aggaattgaa ccagaacatg ctgaagatga acttcaggat gcaattggtg acatgcttgt 47280
ggttcaaact aacattgcag agcgtgaagg tttcactatc tccgatgctg cattcaacgc 47340
ttacctagag atcaaagatc gtaagggtaa gatgattgat ggtacattcg tgaaggaggc 47400
agacctgtga tcaatgtaca aattattgct gactctaaat atggttcaaa tcgtgtaact 47460
acgtttgagc ttgagtatcc gcgatacgtg catagtgagt tgatgactca ccgcgtattc 47520
tcccgtaatg ctgcatcctc tcgtgctatt cccatcgaaa agatgatcga acaggctacc 47580
gagaacacgg ttatcccaat gtggtctaag aaccagaaag gtatgcaagg cgaacttgta 47640
cgtgacctag agactatcaa tgaagcaaac cgtatcttag gtgtgatgct caacgatgtt 47700
gtcgaaggtg tacgagagct aagcgaattg ggtatccata agcagaatgc taaccgctac 47760
cttgaaccat tccagcacat caaaaccatt gttactgcta cggaatttga gaacttcttt 47820
aacttacgtt atcacgaaga tgctcaacca gaaatccgtg cactagctgt cgagatgtat 47880
gctgcgtact gtaagtctaa gcctaagttg ctcaagccga ctgaatggca cctgccttac 47940
gttgatgtac cagtagctta ccctggttca gaatctgata gcagcgatgc tatttcacta 48000
gatgaagcta agaaagtatc agcatcatgc tgtgcccaag ttagctaccg taagaacgat 48060
acttctgttg aaaaggcaga gtttgtctac gatcgtctag taggcggtga accattacat 48120
gcgtctccat ttgaacacgt atgtcgtcct ctagctcaaa gcgaagaaca gcgtggtaac 48180
ctaatcggtt tccgtcagtt acgccagaat attgaggaca aggtatgagc aaatctgtgg 48240
tagatatttt acaactggct caagactgtg tgggtgatcg tgcggcagaa cgagatacag 48300
atgccgaacg tagcatgaag acgtgtattg atgcatttaa tgccatgtat ggcaccgagc 48360
ttaccgaaga acagggatgg atgttcatgg tgttcctgaa ggcttccaga gccaagggag 48420
gtaagttcaa gctggacgac tatgtggata tggcagctta tgcaggttta gcaggagaat 48480
gtcatggctc aaaagaagtt cagtaataat cacggtatca gcctaccctt agcggtatgg 48540
ctggcccgag acgagtatga tcatgatgat agaaccaatg tgatctcagc aactagtttg 48600
ttgaagtcgg ttcgtcagat tgtgctcagt acccgtgtgg tgccaacagc aggtaatatc 48660
gaggatattt ctgataggct tgcttcccgt attggtactg cgttacacaa ctcagtagaa 48720
cacgcatgga cggaaaatta tggacgtgcc cttagtgatc ttggttatcc tcaacatgct 48780
attgatcgtg ttgtggttaa ccctacttcg gttcaaaaga acgatatccc tgtgtatttt 48840
gagcaacgga gtgagaagac ggttggaaaa tggattgtct ctggtcagta cgatctcgtt 48900
tttgacgggg ttgttaatga tgtcaagtct acttctgtat atacgtggat aaacaatacc 48960
aacgacgcta agtataagaa gcaattatct atctaccgat ggcttaaccc tgagattatt 49020
acaaaacctc atggtgcgat ccagttcatc tttaaggact ggaacaaaaa catggcgatg 49080
ggtaaagata aaaaatatcc taaacttcct gtgatggaat acaaactcga actggatcaa 49140
ccatctacca ctgaacagtg ggtgacggac aaattgaacc agatagatga atatttacaa 49200
gtgccggaac ctgagttacc gttgtgtacc ccagaagacc tatggcaatc tgaaccaaag 49260
ttcaagtact attcatcgcc ttcggccaaa cgtgctacca aagtgttcga taacatggcg 49320
gaagcaagac gttacttaat ggttcaaaag aagggaatgg gactagtgaa agaagtatat 49380
ggtgaaccta ctgcttgtaa gttctgtcca tgtgccggta tctgttcaca gttcaaaggg 49440
tttgttaagc aaggtctaat taaataaggg gctatcgccc ctttaatgag tctaacatct 49500
aaggaataac atggaactca aaccattatc cgaggtggac tataatccaa ctcaagagga 49560
tttactccaa gtactgcgaa ccaaaacgca gaacatcgag tcagatctat actttcgtac 49620
aatcatcgct ttctatctat ctcagattgc ttcatcaatg cgtgcaacta ttgttacacc 49680
gcaccgaggc aaactaccag tgaacatgtt tgtatgtgcg ttaatggagt ctggtgccgg 49740
taaaggttat tcaatgaata tcatggaacg agaaatcgta cagggtttca gtgagacttt 49800
ccgtaaacag acattcccaa acgtatctga gcagtctatt gatgacgaag ctcgcagacg 49860
tgcagttcgg aaccaaactg atgaagctga agaacgtgaa gaactctgga aagagtttaa 49920
ctcactaggt gctatgcctt acagctttga ctctggttca ggcccagcct ataagcaagt 49980
gcgaaccaaa gcacagattg ctggtattgg tgccactaac ttcattggtg atgagatcgg 50040
tactaaccta agtagctgga gcgaaatgct tgcagttaac ttggaagctt acgatgttgg 50100
tttaatcaaa cagaaaatca ctaaaaatgg ttcagactct aaacgttatg aagaacgtag 50160
tgacccagta cctgcaaaca tgctggtatt cggtactcct tctaagctgt ttaacggcgg 50220
cattgaagaa cgtgaattca tttcactatt ggagactggt tatggtcgtc gattcctatt 50280
tggtatcggc aagaagactg tagctcagga tatgacagcc gaggagctgt tcgatatctt 50340
aacgaaccac aacagtgata cgctagttac ccaactggct aaccgtttcc aaacactggc 50400
tgacgagatt aattacaatc gcgagattaa aatctctaaa gatgtaacgt tgattaactt 50460
gcagtaccaa ttggactgtg agaaacgtgc atcttctatg tcggtatttg aaccaattcg 50520
taaagcagaa atgcagcacc gttacttcaa agcattgaag ctggctggtg tatacgcatt 50580
cattgattgc acgcctgaga tcacagaaga acaaatgtat gcggctatta agttagttga 50640
agacagtggt aaagcttgtg atcgtatctt gtcgcaggac aagaactatg tacgactagc 50700
gaagtacatt gcagcagttg gtaaagaagt cacatatgct gacctagttg aagaccttcc 50760
attcttcaat ggttcaaaag cagcgaaaga cgatatgatg tcgttggccc gagcatgggg 50820
gcaccgcaac aatgtgatca ttaccaaaaa gtttgatgat ggatttgaga tcttccgagg 50880
tgaaactcta aaagagactg accttaataa cctgatcttc tccgcatcga accacgttgc 50940
ctataactac atgccttcta cgcacaagaa tctcaaggca gtacgctctt gggagaactt 51000
ccatcgtgtt gctacccaag atggtgtgca ttggtgtaat catactttcg aggatgatca 51060
ccgagtagaa gagaaatcta ttcctgaatt caacatggta gttattgatg ttgatgggga 51120
atgttcgtta gagactgcac agatgctcat gcaagagtac aagtgcttct tctacacaac 51180
caaacgtcac actgacgacg agaaccgttt ccgcattgtg gttccactca aataccactt 51240
gaagatgaca gctaatgagt acaaggagtt catgaacaac ttgtttactt ggctaccatt 51300
caaagttgat gagcaaacga accaacgctg taagaagtgg ttaagtcatg caggaggcga 51360
gtcattgtat aacgatggcg aaatcctaga tccaactcag ttcatcccta agacagccaa 51420
aaatgaaaga cgaatggctg aagctcaaga cattggcgat atggataaag ttgaacagtg 51480
gttcgctcgc tctattgaag agggcaaccg caacaacact attttgaaat acgctttaat 51540
gctcctagac gggggcatgg cccctgacga ggctgaaacc aagactattc aattcaacga 51600
taagttgaag aatggtttgt ctaaagatga gttacgaaca acagtcctta aaaccctatg 51660
ggttaaggca caagaacaag ggaaaatcta atggatttat caaataaatc ttactccgaa 51720
ctggttcacc ttcgcgctca aatcaatcaa gcgattgctg agcaagaaac ctctgtcgtt 51780
gaacgtctga acaaaggcga aatcgtaccg aacttccgtt tcaaagaagg tcgtatgaaa 51840
cgtgcagtta cgaaccaagg tatgttggtt caaacgctac gtgacaaagg tctatctaac 51900
gaagacttgt acgaagctaa gttcgtgggc atcccaacgc tagagaaagt gcttaaagct 51960
aaactatctc aagcagatgc tgacaagatc atgaaacgtc atattgaagt ttcatacggt 52020
aatccaactc ttgagtacac aggtgaataa tggctaagca gaacgacaac ttaatcctcg 52080
tagtagggaa gtcggctgct ggtaagtcga tgtgtcttaa agacatcaaa gatcacgaag 52140
gcgtgatgta cttgaactgt gagtctggta agaaattacc attcaaaaac aacttcaagc 52200
gtctaacaat cactgaccct tacatggtat tccaagcttt ccagaaagct gaagaaatgc 52260
cagagattca tacgattgtt attgactccc ttacgtacct aatggatcta tttgagtccc 52320
tatacgtagt acctaaagct ggcaccaaac aaggtatgca agcttggtct gactacgccc 52380
agttctttaa agcattaatg caacaatatg ttgcagcttc tacaaagaac gttatcatga 52440
ctgctcacac tgctgatgaa gttaatgatg acatggtgaa agaaactttg gttaagatca 52500
aaggttctgt catgaacaac ggcgtagagt cttacttctc acaaatcgtg gcatgtaaga 52560
agatgcagct aaagcatcta gacgggtatg aatctgacct gctgaatatc actgaccgtg 52620
agacacgttt gaagcacaag tatgtgttcc agactgacct aacagcagac acgattaacg 52680
aacgtattcg tggcccattt ggaatgtggg aagaaaacga aatcttcatc gacaacaaca 52740
ttcaattagt actcgatcga attcatgact actacggtga gtagtagctg aaccagatta 52800
ccactatggg gactacgtcc ccttttgcca taagtaacac aggaaagaaa caatatgtta 52860
gacgcactaa aactatcacc agatgttgaa ctagaaaatg ataaagatgt acttggtggc 52920
ggtggcccgc ttaccacaga tatctatgca cttacatgtg agtacatgta tctagagcaa 52980
actaaatctg gtgcagttat ggctgtaggt agcttcaaag aagcagactc aggccgcaac 53040
attcgcttca gcgagtgtgt aatgtctaag aaatcaggta cgttgaaagc aacatacact 53100
gacaagaaga ctaagaaaga gcgtccgtta ccgggctaca ctaaggtact acaccttgct 53160
aaagtgattg gcctagacgt agctgactta tctggtctag cgtgtgaagc gaaagttctt 53220
aaactattcg acttcaaaga gcgtaaagaa gtacctcaag agaagaacgt acttactgac 53280
tttaccggtg cagcacttaa agcaggcgtc tacctagttc gtgaatccaa aatggaagaa 53340
agtgaaccag gtaaatggga tcagccgaca ggtgaagagt tcgctaagaa cgatatcgtt 53400
aagtggttca acgaagatgg cctaaccatc gaagaaatga ccaacggtgt tggtgatgaa 53460
ccatttgctg atcagtggcg tgaagctcac aaaggcaaag aacgcgataa ccgtgtgaaa 53520
gacgcagctc ctaagaaagg tgcaccacaa ctaggtggta ctgctccagc aggcggcggt 53580
cttaacttcg actaatgaat aggggagtag ttgtagctcc cctgcgcatt gctaagaata 53640
aaaagggtga taagcagtgg tcagtgaatt tgaaccaata ccggaataca cacttccaaa 53700
gtcttaatag cttgaagaag cgttataaag atctggtata tcttcaagta gatcaccttt 53760
caccctttac ttgtatccga cttaccattc atatctatgc cgccaataaa cgtctattcg 53820
atataggtaa tgttggttca atacacgaga aattcttttt agatgcactc gttgagttgg 53880
ggaaactacc ggatgacgat tacaatcatt gtcccgaaac ccatacgtac tttaaaggga 53940
tcgacaaaga tcaccctaga gtagaattta ttatcgagga aatcccgatg tcattatttc 54000
aaattgacaa gcctgaccgc atcccagtaa acatccgtac cactgaacat gatttggttc 54060
aattggtcaa agaagctatt gccaaagaag atccttctat cgacgtaact gatgttcgtc 54120
tagttggtct tgaagcgatc gagatcgacg catttgtagg cgaggctcct aagaagaaaa 54180
ctacccgtaa gaccactaag aaagctgaac caaagaaaga agaggttccg gctgaagtgt 54240
taaacgaagt agttgaagca caagaaaaga aagaaactga agctgaaaaa ccagttgaga 54300
ctgcggaaga tacagatcta ccgttcgacc ttgacgatgt tacagaccct gaagcttcaa 54360
gtgctgactt aacttttgcc gaccaagagc tatctctgat tgatgaagtt ctagctgaag 54420
actctaacga gggtgcagta gatgaaccaa tcaacactct aagcttcgac acaaatgaag 54480
aagcagaaag tgaaccagaa acaggtggta cgactgtagc tgatatcttt aagtaatcgc 54540
ctatgttcct gaccattccg tttaacccat tcatgatgct cgctttcatg gtgttctgtt 54600
tagtcgtaat cttcttcggc tatcgaatct ttaaatggtt cagaaacgga atgaaacggg 54660
cattcgatga aatagagaaa gaaaagaagg ggcattaagc cccttttttt attagttagt 54720
gaatggtctt agcaatagag cattgctgat catctctaac gggttgtccg gtaatcttac 54780
acgggctgct agagtatcca acgatagtag tgagtcaatc acacttggaa tagctccacc 54840
agtcattgca ttaagtacgt tgaagataat gtttcgtgca gagttctcac ggaagttacg 54900
gataatctgc ttctgaatac ggaagtagta cttagtgaac caaagtacac ccatatcatt 54960
caagtactgt agcaacggtg ttgtcggcat atcgtagttg ataaacacat ccataaccaa 55020
accagcagca tcacgacgac tcataccatc attctctgtc ttgtgcttgt atagcgcgta 55080
acgagataca aagtctgata gctgagctgc ttcattcatg atcttgaata gcttagtgtc 55140
atgtgacatg taaactacct tggcaccatt tcgaatgaat cccggtaagc gtgtagtcaa 55200
cgcttccact ttctgagtta actgtgactt atgagagaat gtatcatcag ccaattccac 55260
atcttctaca atcgactgca acataccatc ttcaatcaag cttccaacag gactagtcgc 55320
taattcgtga cgcagttcag ccatacgctc ttggttctta tccaagttag ctggttcata 55380
ccccaattct acaattttct ctaactcaaa taactcagca gcgtgacgac ggtaacgacg 55440
tgcttctacg taagcctctt tctgccactt caacgcatca ccaatagaca taccttctaa 55500
gaatagctga gaggtatttg atagagcatt catcagggtt actacacccg acttaactac 55560
cacaaattcc ttagtctctt taacaaattc tttccagata tcttctgctt tacgaatcgt 55620
acctgcggct ttcgggccta atacatggtc agcaaattga accaatgctt cacgtaccca 55680
acttgtctga gctttttcac gatcaagcaa gtcagcagca gatagtttac ggttaccgaa 55740
tacaatcgga gccaaatcac gacgaacata catacggctt acaccccaaa catcgcgagc 55800
atatgcttta gcatctggtg gaagagttgc ccagtattct ttcagaactg gatcttccga 55860
cttagccgat acctcaatgt atgcttcaga attagtagca tcgtattctt cgtatagagc 55920
atcaatcacc tgacggttca cttctggtgt tgtgcgttta tccaaagagt atgcagccat 55980
tgcgcccatt acatcatcaa agtggttatg cttcgctagt agagtatcct tagtctcttc 56040
attcatcacg taacggtaag agttgatcgt accatccgca ttgacgttag gaagtaactg 56100
accaccacgt ttagtagttg ggtcaactcg cttagcctga cgcatctcag caatatcttg 56160
ttgcttagaa cctttgatat ccattgggtc aaactcagtt tgagcagaac ctttcgcctt 56220
acgcgaagta agagagatga tacctgccat gtaagcatta gctccaccgt acttattcac 56280
cattaggtat accggttctg aaccaatgat atgagtatct tgttccagtt cacgtactac 56340
ttggtaacct tgcttttcgt attcagcacg ttcagcagaa ttcacttgaa ccacagagat 56400
attgttatct actaggtcac gggtataacc cttaatgaat tttgaaccat ctccatcaaa 56460
tagagttgct cggtcttcct cttgcatctg cttgtgcata tcaagaacgt aaccaacgct 56520
gtgctcgttt tcacgaatca attgaaccaa tgcagcttta tgctctggtg ccgtgtgttt 56580
gatagcgtac agtgaagcaa gcttatcaat cgtaccaaca tggttttccc aatccgcagg 56640
agtagttgtt acgcctagca tcttagcaat gttctcagca tttagcagag tgtgtgcttc 56700
gtacacgtta cctgttgcca tgtagtaacc aagtgcttgt gatgcacgat agtagaaacc 56760
acggttacgg cttgaaccaa gtagacctag agtacgagag atctctttct ctaactcaac 56820
aggactatca agtactgcct cgatttggtt catatctagg taatcccaaa gtgtagccaa 56880
gtcagtctta ataccggctt tagtaattgc tactttttct tcagagttca gtttacgctt 56940
aaatgaacca ttcagtgctt tagctgtatt cgtttcaatc tgcttacgat cgtggtcaag 57000
tagctttttc atctgacgag ctaaatcatg gaatcgtgca gtgctatctg tacgactcat 57060
tgcctcagtc aacagtgaac caaggatcgt ttgacgacca cctttagaat gcaacttagt 57120
ttgggccaag tattccataa agccaaacac accatcgtat gaccaacgca atacgttact 57180
tgctgtacga accactttga agcggttgtt cttcaagaaa ctcttgttac ccatttcggc 57240
agcagtctca cgagtctcat tagtaaactc gccaagtttg ttagccacaa cgtttaccgc 57300
agcatctgca tattgaacca atttgtgctt gttagctgat tcaactccag ccaattccag 57360
tgccaatttc tgaacacctt cagttactgt catatctgat gttaggccag acaagtgacc 57420
atttacccag ttcagagcag tatcaaagat attacgtaga gtctgaaccc aatcaccttc 57480
aacgaataga tctttagaga atacattctt aggcacttga gtacggctaa gtgcattcat 57540
gaacttctcg ttagtcaaag catacgctgc aaactcttgt aggtagttag aacgaccact 57600
tgcttgagca gtagtacgaa tgttcataac gtagttccaa gtgtctttag cacgcttgta 57660
gtcttctgag tccacaccgt tctgtgggtt atccattaag tcttcaacag ttacaagttt 57720
gttctgctta acataacgga acagtttcat tagctcacgt ttagcacgag agttaccgtc 57780
gatagcagca cgtaaaacac tgtgcgtcat ttcgtgagta aacgtctctt gtgcagacat 57840
ttttgaacca tggaacgcag ctacaccaga tggagttgca accgagtcaa ccatgcgtgt 57900
gtaaatgtaa acgtcatcgc ctctgatagc accataagtg ttacctagtg tatctgctag 57960
gtgtaagtta attggcgtca ttacagggcc aaccagcttg cctaataggt cacgtaggtg 58020
ttcacgatga gtagtactct ctgtcttgtt accaaggtta ttcatttggt caaacgtttg 58080
aaccaagttg tcttggctca ccttctgagt attctctcta gcgaagtcac gggtattgaa 58140
tcgtgcagcc gaagcagaga ataagatatc gtctgctgaa agctctgcat caattacttg 58200
aaccgcagtc attactttct caagagcatt gcctttcttc gcatcagtga taccgaacat 58260
ctctaggatt gcacctttaa cccaagcata agcagacttc actttgtttt cgtaagtgat 58320
ctgtgtttga gccaaagcat tctgcatctg ttttgatgtc agagtccaag cgatcatctc 58380
gtcgatgttg tcgatagagt tagccttgaa tgtatcgcgg tgattcttag ccagttcctt 58440
gcggatgtta gccaaatcat tcttagcttt gttcacttca ggattcttag aatctttcat 58500
tcgctcaact agtgcagcgt gaaccaattc gtgagcaata gtttcatcac ttaggccatt 58560
cactgcgtac tcgtcacctg caataagaat caacttctct ttagcaacat gtaggccacg 58620
gctattaacc attgaaccag agtacttcga gtgtttatct gcatcgaaca attctactgt 58680
gtagtcacgt ggtagtaggt cacctagtga tagtagagtc tgacgaatag cacggtcttt 58740
agaatgtttc ttcaagaacg ctacagtctc aacacttgtt tttgaaccag aagctaagaa 58800
ctctttgata ccttcatcca ctggataaat gttctcttgg ttctcagtcg atactgattt 58860
tggttcaggt ttagcagcat acgtatcgtt caaacgagac actacaaact caagttgagt 58920
tggttcaaat acttcaagag cattcttcac tgtaccggca cggtattcag ttttgaagtt 58980
agccaaagca ttagcaaatg cagtagcacg ctcaccacct tgttcggtat aacgatgcat 59040
tagtcgatca acaacaatgt ccatacgacc tttgttacca gtcataccac ggtggatctt 59100
accgtaagct tcttcataag aaatagtctt tggttcagca cctttattac cagttacgta 59160
gccaccattc tccatgtagt actgagacat gtatgtgata cgtggtaata gcgagttacg 59220
gccttcctcg gtatcagtta ctgtatcgtt gattgaagca aggaagttct cccaaccttt 59280
atcagcagca gttaagttag acgctttaac acgatcaata ccgtatcgag tagcttcttc 59340
gttcactacc attaggctac gggcagtttg ttcagagttt agtacgttag taccattctc 59400
ttcatcgaac ttacgtgcag cttctagagc gcgttgtagc gattcattag cttgagccac 59460
gatagagtaa tcgttcataa cttgattaaa gttacggttg attgcttcca tatctgttag 59520
gccattagcc gtaccagtaa agatcgcatc gtgtacgttt agtacgcctg cattttctag 59580
agtaagtagc attgtcgcag catctgtacc ttgtacagaa agtactgttg gacttacacc 59640
accgtctacc ggagtagtta ctcgaccaaa tgcgttacga gtcttagcac catcaattgc 59700
atacttacct ttacgcttag cctctgtacg gaactgagaa gtctccattg ggatattctc 59760
tgtcttacct acaaagatag cttcgttcaa gttaccagat tgtgctgaga aatatgagtg 59820
gattactggc tgtgcctgtg ccaactcttg ttcaacttca cgcatcatac gcagaggtag 59880
tgtctcacgt ttaccagaga tcagaccttt ctcgatagct tcttgagttt tcttctcgat 59940
aagaatatcg cgttgctgtg catacagatc gaatacgact ttcatcatat cgttcacttt 60000
agtacgacga gccataaact cagaatagtt atcttggata gcgttaaata ccgcttcgcc 60060
atagacttct gaaccaaaat taacaatatc gttttcacga cggtatggta gtttgaaatc 60120
caatgggttc ttaactagct cattagtttg agacttagtt agctgaagca ttgaaccaag 60180
attacgaaga tcttgttctg tacgcttgga gtcgccaacg tagtcataca ctttgtctag 60240
tagcatgtgg cccatagctt tagtgatcgc tttctcacct gaaccataga tagctgtcat 60300
cagtgggttc ttagtcaggt tacgacctgc tttagttacc acttcacgat cagtaccact 60360
tagtgcggca gttagctcac ccattactgt gcttagtaga gctgcatcac gttcacttag 60420
gttgtcgata cgtttaacta catcacgagc aattgcttgg tagttatcat cgttcagtgg 60480
atcagaagac ttccaatcac caaactgttg agtatcgcca tctaggttaa tacctacacg 60540
tttcagacgc tcacctacat tgtcatcaga tgcaccgata gcaaactgta gtgaagcaat 60600
agctgggccg ttagtgatac cgtcaccctc gatagagtaa tcagtttcaa actcagtacg 60660
accttggcgt agagccagca tgtactcaga gtaagcaaca agtgcatcca ttgagtgagc 60720
agcttcacct gctttatcaa ctgtagccag aatcttatcc atatcgaatg gcttgtcttc 60780
tagcatcttg attgtctctt ggataccttc attgaatact ggtgtttgaa ccaattcttc 60840
taattcgtca cgagacattt gatcagtctg tttgtcgatg cttacgccaa agccttgagc 60900
aataccaaca agaaacagat cagtattatc catggaaata gactgacgct gatcaccaac 60960
atatagaaga tgacggtgta gcttatcgcc ttgagggtta accgtattgg agacgatacc 61020
gacacgttgg ttcttccact gttcccagtt gaagtagaac ggttgttctt cgttttccaa 61080
gctttcatgg aactctctcc agtatttaac cgagttcatg atttgaccat tcttaccttc 61140
tacaccagca cgttgatgtt gctgaactgt agcagggtct ttaaggccca tcattcgacc 61200
gatttgttca tctgtcattt gagagaatag tttctgttgg ttcttcttaa caaagtgctc 61260
acgttgtgag tgcttcaata agatatcctg tactttctta gccactgact gacgagtacc 61320
tttctgacga gttactgtct tagttggttt ctcagtagtt gggccacggc ggaatgtttc 61380
agttttgaac agacgaccaa gtacatcatt tgaaccagaa gtatcttcac cgaatgaacg 61440
agcattatca gtgtctacca ttgagtagaa aaagatctca cgattctcat caatatcttt 61500
agctgtgttt gtgtcatcca aaccatcaac aatacgtgcc agttcttttt ggttcacttt 61560
gttaagttgg attaggccat taccacgaag catatctaga ccaaatagac ctaaagatgt 61620
ttctaagtta gtttgggcat tgctacctgc atccgggcca gctttgatac caagagttct 61680
aacaatagct gaaccaagtt catcaatgat gtttgactgg tgagtaccga cgttctgtag 61740
tagatcacgt agctcaggag aaacatactg ttcttcttga cgaccaacaa tgttgttgat 61800
gtcttcttgg ctattaaacg tagtctctgg agctttatga gccatccagt tatacatagc 61860
caaagcaaca gatgctttca cgttctcatc aaatgaacca tcgtcattac gtaggtaaag 61920
catgtaatca gccggtgcat aatcacctgc cgcagcttca tcaaactcag cactaaccgc 61980
tttagcgaat tcagtaaatg aaccaattgc acgagactgt tcttcagtta acgtgtcttg 62040
gtctaaatac tcaactgctt tgttcacatc agctgatagt gtttcaactg cgtttggttc 62100
aacttgaagt gggctattag ttagaccttc ttcagcaaca gtagatacga acttctcacg 62160
aaccacattg gtttcagcaa atgctttatc agcttgctca ggtgaacgtt cttctgtagt 62220
tggttcaaca gtacgagtag ttaagcgttc tgccaactta ggagttggtt cagttacttc 62280
agcttgctcg acagtttcac taataggttc cacatcagca gtagtagtaa catcgctagt 62340
aggagcttca tctacagtaa cctctggttc aatagtagtt tcagtttcaa cagcagccgg 62400
agtctctact tctgtttcca caggagcagc cacgctgtca gtagtatcaa cagcaggagt 62460
ttccacagtt ccattttgta cctcacgtaa accatttagc atagtttgta gtgcagtagc 62520
cgcttctgct tcttgttcaa tctggttcac tagagaaggt gcaatacctt ctgtcttcag 62580
atcaatagaa cgcaatagtt gagcacgttc agcatcatca gtcgtattta gagcctgacg 62640
agccacagca gctttgtttt ggcgttgggc tacaaagtta tcaagatctg taacataacg 62700
ctgtacagca gcctcattgc caccttcgat agcattagaa atgatttcct gatactcgtt 62760
caaaccacga taacccggac taccttcaaa cacgtcttta gatacttcac cgatagattt 62820
attcagacgg ttaaagtcac gtgcagcagt tactacagta cgctgttctt cagttaagtt 62880
agttgaacca agtagagctt cagcgttttc atcgcttaga gattctgggt cgattaggat 62940
agatccaata actcgctcag tagctccagt atcgcctgcc accacttgtt gcgttgcttc 63000
ttcgatgttg tttgtttcaa gttgagcctt ttgtagttga gacatgatat tgctgtaacg 63060
acgaccctca gtacgcaatg cgtcagcacg cttctggaat tgagtcgctt gctcaccctc 63120
ggcagcatta gccgcttctt caagtgtagc aatctgttca gcatagtcag tccatacttt 63180
tacagcagct tcatagttct gtactttctc ttcttgggtt acaccttcaa cattgttgcg 63240
agatgcaata ccacgcagag ttccaccaat tgagaagttt ggatcattga catcagttgt 63300
aacgctctct gttccatttt cttcaatatc ctttttagtt gtacggttct gtttagccgc 63360
tttagcttta tcagttactg aaccagtagc ctcaatgatt gaagcagcag tgttcgcagc 63420
aacgccagaa gcaccacctg atactgcggc agcaaccgca ccagtagcag cagcttcacc 63480
aacaccttca gttaggtctt gggtttcatc taccgtttgt tgtttagcaa tgttagtagc 63540
tacttgtgaa ccaccagatg cacctgtctc ttctaatgct tctttagtag caccttgagc 63600
tgtagcagct aggttactta gtgtcttacc gacacgtgaa gcagggttga acaagttagc 63660
agagaattca ccaccaccag ttagacgaga tgatacgcca gcaaatagtg ccgagtaacc 63720
aaatgccaag ttaccagcag catcttgtag acgagctttc gcttcttcat ggcttaggcc 63780
atctgcaata aggttacgat actcaggaga attttgaacc aattcttcct cagtcatctt 63840
ctcgatttca gtaagtactt gtgcttgtga accaagacct tcttgtactg cagtagttgt 63900
tacacccaaa gcagtagcac gtttcttcag ttctttttga cctgcttctg tagcagccca 63960
cttagcaact gactctgcac cttgctcagc aatttctcgt ggcacagcca aagctttacc 64020
gataccacct gttaggaaga tatcagccac gttctcaaat gctaagtcga tagccaaaga 64080
tggacgactt gcgtatgcag caacagtgtt aaagaattca ccagcaactt ctttagcttt 64140
ctcagtacca gataactctc gatcaccgat ttcagcttta cgttcttcgc ggtatgcttc 64200
cagttcttct tggttagcaa agcgtcgagc ctgaattggt tcagactggg tattattgaa 64260
atcgtcaact agagtctctg catctttaag agcagtaaga atagtattac gttcttcttg 64320
gcgttgttga accagatctt cacgagtctg agtttcacga ccttgctctg ccagtgcagc 64380
ttgtgcacgt tgaaagtaag ggttctctag aacgtcatct acttctgcgt caggtaatgc 64440
agcacctaat gctgattgtc ctaaacgaac tgcaccacga cctgcggcaa tagcagcatc 64500
tgaaccaaat tcagttaatg aacgagtacg atcatctcga cgtaaacggt tctcagcagt 64560
attcgcataa tcacgaatct ccttgagagc agccatagtc ggatctaatt ccaattcaat 64620
ttgattagaa cgttctttaa cttgttgttg ttttgaacca cgtttctgag aaagttcagc 64680
ctgtttctga ctagacttac tattgcccga agccaatgca cggaactgtg ctagttggtc 64740
ttcagtagtg gtatttggtt caaatgtaga agccaagttc tgacgacgtt cttccaaacg 64800
ttgttgaact agttggtctt gcgcagattc ttgttcagga gtgaactgac cttcttcagg 64860
taagtcgatt ggattttggc gttcttcagc cataagataa atctccttga attgatccgt 64920
acattttcga tgtagtgtaa tacgttatca aggagaaata caggaaatgt gaaggggaac 64980
acagttcccc ctctatttag ttaatgtcac ggatggtttt acgcatttct tctactttag 65040
aaggaacatt gtaagtacgc gctcgtaatg cagcaggaac atcccaagct ttaccttgac 65100
ggatttgacg agtcattgct gcctcattct ttttgatttg gctctgtaag tcagccatac 65160
cagcatccat cacgtttaga gcaaggtttg ccttacgtac tgcatcttca tcagtgatta 65220
gattgtagta ctcacgggta gcatctcgga tatttgattc agagagccag cctgtcttat 65280
tacgatcagc tcggcctaag atgtaagcca tctcagcacc agttggttca cgatcaccta 65340
gttcctgttt agcaacacct aagtaatcag caaagacttc tttacccgaa tcaagtgccc 65400
actcaagtcg aggtgcataa cgcttagata ctgtctcaag agaagtctct gtatcgttac 65460
gataattgaa tacttctggt cgagcaccaa gtgtttgagc cgcttcttct tgaatagcct 65520
ccatctcacc ttcgaactgt ttaagagcct gttgaccata acgttcttgg gtttcgtatg 65580
tttgacgttg ttcaggtgac cacgagttgt aatactcttc atggaacttc agagcttcag 65640
tacgaacctt gttagcttct tcagtagaca agtcagtacg tttagctaac tgtgtagtca 65700
ggaagtcacc ataagcatta gcatcaccct caaagcgttt aagcgtctct agagtacgat 65760
agcttgaact tagcaagtct ttctgttgct gaatacctgc tgccgtatta gttgtcttag 65820
gcggcaccgt ataattacca atatgttcta ctcggcctgt attcgggtct tcacgtaaga 65880
tctgaccatt ctcatactta aacccatatt ggttagcctg ttgttgttgc aactggagtt 65940
tacgatcatt caaatcgtta ttgatctctt gctgctccaa cacattctga aacttctgtt 66000
gctccagatt acgatcaatc aaagtatctt ggcgtgaacc aaaaacctga ttaattgctg 66060
tctggtctac ttggttaccg aactgactag agtccacacc acttaaagct tcttgtgaag 66120
tagcgcggtt caactgatca ataatattgg cagtgttacg ctctttctgt gcttcatagt 66180
tagtttggtt cacattggtc tgttcttggg ccaaggaacg taagtcatta attgactgag 66240
agatacgatc agcacctgca tcgaaagcac ccaaggcacc acctgcatca ggtacttgaa 66300
cgttatccca tcggattggt cgagccatgg gttactcctt ataaacgact tttcttctgg 66360
ttgttacgta cttcagcata cgcagcttct tccgcagtac ggcgatcacg ttgatactcg 66420
aactcttcac gttgtagttc ccaagcttca cgctgtagac ctaattggtc tttagcaata 66480
cctagttgct ggaagccgag ccaagagtta gcaagtgtac caacggcacc gatagttgga 66540
gccaaagcac cagtagagaa attaccctgc tcatcagtac caccgaacag agtgttaaat 66600
gtactcatag ggttagtacc ttgagtgttt gaaccaattc cacctgtatc gaaactgtaa 66660
cctgagctag agccgacacc gtagttattg ctgtagttat tgtccattgc taggttaaag 66720
ccttgcatag cgtttgaacc taaaccgtaa tgatttggca tatggagatc tcctatactt 66780
taatgcccat gtgggtagct actccctcaa gggaaatcat gttgtctacg tagttttcaa 66840
tatgtgcttt atcaattgga gccaagttgc ccaagtgaat acgagtagta tagtactgtt 66900
ctgctgtctc atttggaaga gccaagccag accttgaatc cgggatacct aacggatcaa 66960
tcagaaatgc agtacttgtc tcagcataga actggttcat ttcttcttcg tactcattca 67020
tctctcgttg taaagcatcg tagtcatcca tgatatcact catttcagta tcaatgtaag 67080
cgttaacgcc tgtttcgata ccagactgta atgcacttaa cgtttgggcc agacctgtct 67140
ggtcaccacc aactgaacca agagcgatcg aagcgaagat agcaaacaca acaccagcag 67200
ctacaccaag ctcatcgacc aaatagttga taccatattt gataccaacc gtaatgaccc 67260
ctagagcgat ggcacctacc acagccgcag tcaaacctgt accaaggcca agccatgcac 67320
ccatagaggc agaggcagta ccaaaggtat aaatggtcag tacaatacca acaaccgcta 67380
caatggctga gaacaagcct gtttcgtacc atttaagctt ctgtttcaca taacagttga 67440
agttgatacg catagcttcg ttcactaagt cagtacggtc tacaacacgc atattttcaa 67500
ctagacgaat gtccaaaggg ataagcatac cgtctgagaa gtcatcttca ttctgtgccg 67560
cgctaactac gttagctaat gaggtatcta ctgtatgtga tcggtaaaca tagttaatgt 67620
ggttcaaacc acgtacttca acttcatcat atgaattggc agtaatctgc ttacgtacaa 67680
cgaaagaact atcttcataa ccgtagtact gaccagtaac ttgaggacga acaatgatct 67740
cagtgttcac atcatctggt tcagcatctg gttcaattac accagttagc aaagtagatt 67800
taatgtagag ccaacctagc tcaacatcat atgaagtacc tgcctcggaa atacgtaagc 67860
tgttaatcgg tggtggagca gtcagtttga aatcttcatc tctgtcattc cactgatcat 67920
atgcttcttt gtcataggta gagttattct ctttatcttt ccagaagtcg tacaggtagc 67980
tttgtgcagc tttaatctgt gtatttggag agatgcccat taatacgtaa gcatgatcaa 68040
tatcaccaat atccgggtta gcattaaccg cactacctag ttcagagata ctcagcttta 68100
actgtcttag taaacgtcga gaagtgttat agcgttctga accatcatct actgtaagat 68160
cagcattgtt ataccgaaga ggaacaacag gaaagtaatt agagtcaaac tcttcgtaag 68220
gttccagtac ttcataggtg ccctcgtcgg gagaatagaa ccagtaataa ataactgaaa 68280
gatctacttc ctcgtttgca tcgtatttgt agtacccaac gtggtaatac ttagcatctt 68340
ctaagattga accagaatag gtatatgtct cagtgaactc atttaagaat tcatctgtat 68400
aggtaagttc aatttggttc gtactaacga tctttgcgta agagaatact gtttcgcctt 68460
gagcacctga agggggatta cttactacta atgtagtttg gttcaatcct cgtgtaccta 68520
ataagtactc aagagcaaag tagtatccat ctgctgtact gtagattgaa aagtctacaa 68580
gcacatcctc tccgatttct tcagagataa cattctgaac ggtagtaggt gaagcacctt 68640
cattgttcat tgaaccagaa ggcactccat aatagtactc ttcagatccg taccggtaat 68700
atctcttcag gttggttccc aatccactaa tggtattggc aatcaaatca tcggtgatac 68760
tacgttcagc acggatagaa gtaagtactg attgttgaac caaattgggt acttcctcaa 68820
ctagcggagt cgagaaggac tccacataaa ttttagtctt tgaaaataat cccattggtt 68880
caatccttat gcttgtacag ttacgccaac accttcacga actttgttca agacatcttt 68940
gatctgtgta cctgttacgg tagcatccag agtttcgttc tcgtttagag actcgataga 69000
tgtacgggtg ttgaatgagt ccataaatag cttagcagct ttctgctcag catcacggat 69060
gtaaccttca gcttggttct catagagagc catttgctta ccgatgatac cacctgccgc 69120
agcaccaagt actgtgtctt tggtttggta ttcttcagta acacgcttct gtgtaagtac 69180
agcagtttct gcatcaagct tagaaccttg tttaacaatg ttagcttgct ctgcttgtag 69240
gttagcttct tgctgaccag ctagagatgc ttgtgcttct gtctgtagga tttgtgcatc 69300
tttaacttct agatctttat ctaggttaag agcttgctta tccagaatca gttcttgctt 69360
aacagcattc tgcagttctt gatccagtag caattgacgc ttcgtttcag aggttgtttg 69420
ctcattaaca aaggcaatat cggcagttag tttatcaatc tgcttttgaa ccaattctac 69480
attcttctcg gcagcaagaa tctgcttgtc caagagtcgg atgttttggt tcgtaatctg 69540
gtgttgaaga aggtagttaa cagagttagc taaagtacta tctaaagcag caatgtatga 69600
cttggtatac tgatcaccag taatacgtcc ggtcttatgt tcttccgcca gatgagactt 69660
aactgcacgc atcaattcgt caaaggcacc tgaaccagtt ctctcaccag aagtaagtga 69720
gtcaatggtt agtacctctg gatcaaagga tgtaatcgag gtcatctttt ttctcctata 69780
aaaaaggctc cacttattgt gaagcccttt ggttcaaaag tctaccggcc ttagtcgata 69840
gcttgacgtt tagcttggtc agcagctagt gcatctaact cttcttgagt caacggatct 69900
aagatctcaa tgttgtactc tttagggaag aagttcttac gatacttacc acctttacca 69960
tctggaacgt ctttagtctg acggaacact ttgttcttca gtgtatcaat catcatttga 70020
gataggtgat actcgtggtc aaacagaacg tatttagtgt ggaagcctag aactgagtta 70080
ccaatcgaca tccactcacc ttgccattca cgcttgtgag ggttcataca ggtgatacga 70140
cagcgaacaa gtttaagttt gtctttacgt agttgattac gtttatccat cttagtcttg 70200
ttaggggatg ctttaacagt atttgcttca gcagcctttt catcgaattc tttaatcttt 70260
tctttgagtt tctctaaaga gattgaagga tggaatttaa tgcccttgtt ctcggcaatt 70320
tgtttaagac tgtctagttc tgtgaattct tcagacataa tatgtacctt ttagttttgt 70380
atccgttcct gatctaagca ggagggtcaa aggctcccct aaagagagga gccatattta 70440
ttacagttca gctactgtct tagctagagc aatacgctct ggacgtagga tcagtgtacc 70500
gtagtaccac tggattgaac tgaagccagt acgaccgaat gggtcgtggt gttgtgcaac 70560
accttcacca ggcatcttag tgataatctt gaacttgaat gacttaccag aagtttggaa 70620
gccgatagtt gtgaacgatt ctgaaccaat acatagcatt gggaagatat cgtatgaacc 70680
accagtctca cggtaaccac cgttagtagt tactgcttta ccagcaccag cccagtgagt 70740
catctcaggc acaactacga tacggaattg accgacttta ccgatctcac cgtgaatcat 70800
gttcacgcct tttttgtagt cgccagcgta tgcgtactgt tctacaggaa caaatgcttt 70860
gttgccaaat gcatcagtca ttttctcgat ttgcatttgt agttctgaac caacgtaaag 70920
aacacgacct gcaccgatag tagaggtatc agtcatacgt gaaccagtta ggatcttcgt 70980
agacttagga gtacggttgt catccaactg gattgacata cgttgtagat cttcgtaagt 71040
gattactgat ttaacgtcag catcttcacc agtcatttcg atgtcttgag ttgcgtcacc 71100
accgaagtag ataacaccag caccgtttag aaggtcaatt tgtagaacgt cttcagtgat 71160
ctcagatgca ccacgaacca attcacgaga aatgtgttgg tataggtcat ccattgtatc 71220
gaagtttaga gcttcatctg tgaactcatg gaagaaacca aggttctcta gattcgcttc 71280
tagcaccaaa cgtgagtaac caacacggtt cacacgacca ccagtttcag aaagaagtgg 71340
tagcttagcg ttgatagtac cgatatcttt gcttgaacca tataggttac catcagcaat 71400
agttgcaccg gcagcgtcga taccttggtc gttcacgtta cgatcatcta gcaatggaag 71460
catatggtat ttcttaattg tcttaccaaa gtgcttaggc attgctgtta catcagcaag 71520
tggttggaag tactgttctc gaacggcttc aatcagagcc attttgtggt aatagtcctt 71580
gcggatctgt tcaccagcag aagagttagt accgtcaccc cacttaatct ggttagtgtc 71640
atttagagcc atagtaatct cctagtaagt ctctattaaa ttaaagtttg atcttctcaa 71700
actcttcatc agacatattc caccaatcat gtgactcagg ttgtggctta cgtcctttgt 71760
tctgagaaga acttgcctta cggcgtttat cttgcagttg cgtttcatca gctttaggct 71820
tcggtttacg aaccactttc ttaggcttct gttgtggctc ctgtggttgg gagtcattga 71880
acgcaccttg agaaaacaat ttgttaccta ctacctgata tgcctctaca tctgaaaggc 71940
cagctaaacc acctaacatt cgttcacgct gcaactcagt ggaaatcttg tcataaatgc 72000
cattggcttt atgttcattc atgattgaca gcaattgggg tttgcttgca agttcacgct 72060
tactagactc atcccagtta gaaacgacat tcattgtgtc tgaaaatgta ggagtcgagc 72120
gaatgttctc gattacttca tccagttcca cctcattgtc gccaactgca tagttactag 72180
gtgtgtactc tttaccttcc tccagttcca taacgtcaat gccgttagtt tgaaccagtt 72240
gggcaatagc gtctttgtta ccgttcatta aatcaatagc gaatgataac ttggactcgt 72300
caagaagctc attgttggca agcatcttaa cgtacttacg ctgttctttg agatctttag 72360
tcttacgggc ataatccaaa cccatcgaca taagtctacg tgcttcagca acgtctttag 72420
gttgaaactc tgaaccatga tgcttcaaag gagacatcag ttcttcgtac aacttatcaa 72480
attcttcttt tgaaccatct tctgtttcgt cgtctgagtc ttcttcagtc tcatcgtcgt 72540
catcagattc ttgatcatca tcttctgaac cagtttcgtc atcttcgtcg tcactgtcat 72600
cttcatcaga ctctgaatca tcttcttcag aatttggttc aaattcgtct tgatctgtat 72660
cttctgtgat ttctagatcg tcgtcaagac cttctgaacc attttcttct tctggttcaa 72720
aatcatcttc actagaattg tctacagcat caagttcatt attcagttca ccgccttctt 72780
ctcgatcacg gatttcagaa tcaacagaag gctcactagg agcctccgcg aataactgat 72840
ctggatctgc gctcaagaaa tcatcgtctg acatagaata aagattgtta ttcgtagttg 72900
tcataggatt actcctcgtt tactaggtct agcttagttt gctctagttc ttcaagtgca 72960
tgtttggcat gttggccttg catgtgtact tggtggaaga acatttctag ctgagcaaca 73020
ccagtcatac ctagctctgc tgctttacgc tgacgctcgt caggagaagc aaatagtgca 73080
gtaagcgtct taggcttatc ggcaatatac ttcttgagga ttagcttttt aaaagcagaa 73140
ctgcgttcta gtttttctag tgcttcagca agttcaactg cttctttagc gtcttcaatg 73200
gatacttcga tttcttgtag atcagtcatg ttaattactc cgtgtccccg gcatcttgct 73260
cgggattatc ttgaggtgtg ttttggttca aagcatgttc gaatgcttta agtttcatgt 73320
taccttccgc ttgagcttgt tgtttctgta ggtcacgtgc atggttcaca ccattctctt 73380
ctgaaacata atccaagttc ttacgatcgg tatcagcttg tttgttacca gtttctgcca 73440
agatcttctg gatctctgct tgcgtcttct ggatttccat ttgctgagcc tgaatgtcaa 73500
gttgtagctt ctgcatctcc atttgttgtt gtggatctgg ttcagcttca tattgacgaa 73560
ttctctctgc tagttctggc attttacgta aatcagcaat ttctgcaaga attgttttat 73620
acatgcctaa accgaagctt tgaccggttg tttgaagcat aaatgacaat tcctgagctt 73680
tttgctcatc tgcctgtgcc gtactgattg ttaatcttag gtcaaattga cccattaagt 73740
catcacgtct tacagtaaca aacttattat ttgtgatacg gatcacttct tcttcgctta 73800
ggaacgtttg gttcatagca ataatctgac gtgcagcttt cttcaagcca ttagctagac 73860
gacgaagaat acccaattca cgacgagctg ctgcatcaag tgcaccttga gcatgtgtag 73920
ctgtctcacc taaaccagca ccagtaatac cttgtgcgaa tgctttaaca ccagataatg 73980
actcagcttc ttgctgttgc attgtaatca tgttgtatgc agacattggt acttctgggt 74040
attggtgcat atagattgag ttctgtgcat caccaacatc gttatattcg tagtctagac 74100
cagcatcaaa acgacgacgg ttcattgggt ctagggcacc tttacgaata cctgtttgac 74160
cgttagcact acgtgccatt aagtcgatca taccacgagt tactgcgcca attactttct 74220
ggttatcagc gataagttca ccatctggtt caccataaac tgaaccatct acaggaagat 74280
agggaactgt cacaaatgga attttaccat gaggatatgg gttctcttcc agacggatga 74340
tagttgaacc aacccaagta caaacgattg ggcgagtttc acctgaacca tctacgtccc 74400
agtaacccca gtattcataa gctactaatt tctgacgagg tttatccgag aacataaatc 74460
cattttcagc ccagttactt gcatggtcac catcagataa cggtgtgttt gaaccagaaa 74520
cgattgaatc cagattctca taaatacctg ctttacgtag ctcagcaagt gaagtttcaa 74580
acgagtagat aacgaactgt gctttctcaa tatcaccatt acaagatgga tcaataatca 74640
cattctcttg gtcacatact tctagagtcg gttggttcat tgttactaca gtttcttctt 74700
cctcgacttc accagtctta tgaggatata 74730

Claims (1)

1.一株高裂解性溶藻弧菌噬菌体(Vibrio Alginolyticus phage)RDP-VP-19001，其宿主为溶藻弧菌(Vibrio Alginolyticus)BV-19002，透过电镜观察，该噬菌体头部为廿面体，且立体对称，短尾，噬菌体长度为78.889nm，为有尾噬菌体目(Caudovirales)短尾噬菌体科(Podoviridae)，基因全长74730bp，不具有毒力基因和溶原性基因，保藏编号CGMCCNo.19980。
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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20190099459A1 (en) * 2016-04-01 2019-04-04 Trustees Of Tufts College Methods and compositions for preventing infection by a Vibrio species
CN110684741A (zh) * 2019-08-13 2020-01-14 广东海大畜牧兽医研究院有限公司 一种弧菌噬菌体及其应用

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20190099459A1 (en) * 2016-04-01 2019-04-04 Trustees Of Tufts College Methods and compositions for preventing infection by a Vibrio species
CN110684741A (zh) * 2019-08-13 2020-01-14 广东海大畜牧兽医研究院有限公司 一种弧菌噬菌体及其应用

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Phage Endolysins as Potential Antimicrobials against Multidrug Resistant Vibrio alginolyticus and Vibrio parahaemolyticus: Current Status of Research and Challenges Ahead;Nandita Matamp and Sarita G. Bhat;《Microorganisms》;20190318;第1-11页 *
溶藻弧菌烈性噬菌体分离鉴定及其抑菌作用研究;欧阳敏 等人;《扬州大学学报（农业与生命科学版）》;20190731;第40卷(第4期);第74-79页 *

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