CN111990255B - 一种葛根组培苗叶片愈伤组织诱导及再生方法 - Google Patents
一种葛根组培苗叶片愈伤组织诱导及再生方法 Download PDFInfo
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- CN111990255B CN111990255B CN202010901257.XA CN202010901257A CN111990255B CN 111990255 B CN111990255 B CN 111990255B CN 202010901257 A CN202010901257 A CN 202010901257A CN 111990255 B CN111990255 B CN 111990255B
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Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
Abstract
Description
组别 | 污染率(%) | 死亡率(%) | 愈伤组织诱导率(%) |
第一组 | 0 | 1.0 | 99.0 |
第二组 | 0 | 0.5 | 99.5 |
第三组 | 0 | 1.0 | 99.0 |
第四组 | 12.5 | 7.0 | 80.5 |
组别 | 愈伤组织诱导率(%) | 愈伤组织分化率(%) |
第一组 | 99.0 | 75.0 |
第二组 | 12.5 | 0 |
第三组 | 25.0 | 2.0 |
第四组 | 85.0 | 70.0 |
第五组 | 65.0 | 50.0 |
第六组 | 100 | 32.5 |
第七组 | 99.0 | 65.0 |
第八组 | 100 | 55.0 |
第九组 | 100 | 20.5 |
组别 | 愈伤组织诱导率(%) | 愈伤组织分化率(%) |
第一组 | 99.0 | 75.0 |
第二组 | 41.0 | 6.0 |
第三组 | 65.0 | 55.0 |
第四组 | 50.0 | 33.5 |
第五组 | 65.0 | 10.5 |
第六组 | 75.0 | 61.0 |
第七组 | 55.0 | 40.0 |
Claims (6)
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CN202010901257.XA CN111990255B (zh) | 2020-08-31 | 2020-08-31 | 一种葛根组培苗叶片愈伤组织诱导及再生方法 |
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CN112715358B (zh) * | 2020-12-29 | 2022-11-01 | 贵州大学 | 一种粉葛愈伤组织的诱导方法 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102144555A (zh) * | 2011-01-27 | 2011-08-10 | 中国科学院武汉植物园 | 一种葛根的快速繁殖方法 |
CN108064699A (zh) * | 2018-02-09 | 2018-05-25 | 周长生 | 一种葛根植物的组织离体培养繁殖方法 |
CN111149704A (zh) * | 2020-03-13 | 2020-05-15 | 广西壮族自治区农业科学院 | 一种粉葛单芽茎段增殖及一步成苗培养方法 |
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2020
- 2020-08-31 CN CN202010901257.XA patent/CN111990255B/zh active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102144555A (zh) * | 2011-01-27 | 2011-08-10 | 中国科学院武汉植物园 | 一种葛根的快速繁殖方法 |
CN108064699A (zh) * | 2018-02-09 | 2018-05-25 | 周长生 | 一种葛根植物的组织离体培养繁殖方法 |
CN111149704A (zh) * | 2020-03-13 | 2020-05-15 | 广西壮族自治区农业科学院 | 一种粉葛单芽茎段增殖及一步成苗培养方法 |
Non-Patent Citations (4)
Title |
---|
In vitro propagation of Pueraria tuberosa (Roxb.ex Willd.)DC.;Bindu T.K.等;《Tropical plant research》;20171231;第4卷(第3期);第480-485页 * |
三裂野葛的组织培养和植株再生;施和平等;《植物生理学通讯》;19981231;第34卷(第6期);第444页 * |
野葛叶片和茎段高频再生体系的建立;洪森荣等;《植物研究》;20080731;第28卷(第4期);第458-464,508页 * |
野葛愈伤组织诱导及分化的研究;洪森荣等;《安徽农业科学》;20071231;第35卷(第12期);第3493-3494页 * |
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Application publication date: 20201127 Assignee: Guangxi Hongge Biotechnology Co.,Ltd. Assignor: GUANGXI ZHUANG AUTONOMOUS REGION ACADEMY OF AGRICULTURAL SCIENCES Contract record no.: X2023980044716 Denomination of invention: A Method for Inducing and Regenerating Callus from Leaves of Kudzu Root Tissue Culture Seedlings Granted publication date: 20211102 License type: Common License Record date: 20231030 |
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Application publication date: 20201127 Assignee: Fuchuan Taro Union Planting and Breeding Professional Cooperative Assignor: GUANGXI ZHUANG AUTONOMOUS REGION ACADEMY OF AGRICULTURAL SCIENCES Contract record no.: X2023980046561 Denomination of invention: A Method for Inducing and Regenerating Callus from Leaves of Kudzu Root Tissue Culture Seedlings Granted publication date: 20211102 License type: Common License Record date: 20231108 Application publication date: 20201127 Assignee: Guangxi Fuchuan Dexin Ecological Agriculture Development Co.,Ltd. Assignor: GUANGXI ZHUANG AUTONOMOUS REGION ACADEMY OF AGRICULTURAL SCIENCES Contract record no.: X2023980046559 Denomination of invention: A Method for Inducing and Regenerating Callus from Leaves of Kudzu Root Tissue Culture Seedlings Granted publication date: 20211102 License type: Common License Record date: 20231108 Application publication date: 20201127 Assignee: Guilin Guanyang SHANGNONG Agricultural Development Co.,Ltd. Assignor: GUANGXI ZHUANG AUTONOMOUS REGION ACADEMY OF AGRICULTURAL SCIENCES Contract record no.: X2023980046558 Denomination of invention: A Method for Inducing and Regenerating Callus from Leaves of Kudzu Root Tissue Culture Seedlings Granted publication date: 20211102 License type: Common License Record date: 20231108 Application publication date: 20201127 Assignee: Guangxi Shunteng Agricultural Products Co.,Ltd. Assignor: GUANGXI ZHUANG AUTONOMOUS REGION ACADEMY OF AGRICULTURAL SCIENCES Contract record no.: X2023980046557 Denomination of invention: A Method for Inducing and Regenerating Callus from Leaves of Kudzu Root Tissue Culture Seedlings Granted publication date: 20211102 License type: Common License Record date: 20231108 Application publication date: 20201127 Assignee: Guangxi Hezhou Funong Ecological Agriculture Co.,Ltd. Assignor: GUANGXI ZHUANG AUTONOMOUS REGION ACADEMY OF AGRICULTURAL SCIENCES Contract record no.: X2023980046556 Denomination of invention: A Method for Inducing and Regenerating Callus from Leaves of Kudzu Root Tissue Culture Seedlings Granted publication date: 20211102 License type: Common License Record date: 20231108 |