CN111956562B

CN111956562B - Moisturizing liquid and mask moisturizing cotton batten

Info

Publication number: CN111956562B
Application number: CN202011025256.XA
Authority: CN
Inventors: 肖慧; 郭学平; 杨桂兰; 蔺虓霄; 邵萌
Original assignee: Shandong Bloomage Hyinc Biopharm Co Ltd
Current assignee: Bloomage Biotech Co Ltd
Priority date: 2020-09-25
Filing date: 2020-09-25
Publication date: 2023-03-21
Anticipated expiration: 2040-09-25
Also published as: CN111956562A

Abstract

The invention provides a moisturizing liquid which comprises high molecular weight hyaluronic acid, low molecular weight hyaluronic acid, hydrolyzed hyaluronic acid, ectoin, caffeine, tartaric acid, chamomile extract and ethylenediaminetetraacetic acid. The invention also provides a mask moisturizing cotton batten made of the moisturizing liquid. Compared with the prior art, the invention has the following beneficial effects: according to the mask moisturizing cotton batten provided by the invention, high molecular weight hyaluronic acid, low molecular weight hyaluronic acid and hydrolyzed hyaluronic acid are combined according to a certain proportion for compounding, the proportion of molecules is scientific, the macromolecular hyaluronic acid can form a film and can prevent water from evaporating, the molecular weight of the low molecular weight hyaluronic acid is relatively small, and the low molecular weight hyaluronic acid is compounded with low-concentration tartaric acid in a specific proportion, so that the use amount of the tartaric acid is reduced, the permeability can be improved, the transdermal absorption of hyaluronic acid, ectoine and chamomile extracts is promoted, the skin repair is accelerated, and the red swelling and allergic symptoms are relieved.

Description

Moisturizing liquid and mask moisturizing cotton batten

Technical Field

The invention belongs to the field of biological medicines, and particularly relates to moisturizing liquid and a mask moisturizing cotton batten.

Background

In spring and autumn, respiratory infectious diseases such as influenza are popular, and a mask with the capability of preventing viruses and bacteria is very necessary to be worn in public places. In addition, it is also necessary for medical staff, patients, relatives of exploratory diseases, and all people in special periods to wear masks to maintain the hygiene and safety of individuals and others, and to avoid viral infection in the air. The mask is scientifically worn, so that the influenza and epidemic can be effectively prevented, the mask can protect oneself and is beneficial to public health, and particularly, the mask needs to be worn by people in a very period.

Most of protective masks commonly used at present are attached to the face, pressure injury and red swelling of the face, local skin and subcutaneous tissues are easily caused after medical workers and part of people with sensitive skin wear the masks for a long time, and the protective masks belong to pressure injury related to medical instruments and mainly appear at long-time pressed parts, such as the nose, cheeks or the back of auricles. When the mask is worn for a long time, skin allergy is easily caused, and facial skin is damaged. Moreover, the mask mainly plays a role of separation, and if the mask is poor in sealing performance, the separation effect is greatly reduced.

Patent CN104510031A discloses a humidifying sheet and a humidifying mask containing the same, which is prepared by including a moisturizing component in the humidifying sheet, wherein the moisturizing component is one or more of hyaluronic acid, hydroxyethyl cellulose, trimethylglycine and ethyl p-hydroxybenzoate, and optionally adding a medicinal component, and the medicinal component is one of peppermint, eucalyptus oil or natural borneol. This humidification gauze mask through put the humidification piece that contains the moisturizing composition in the interlayer of gauze mask body for provide the required moisture of human nasal cavity, keep the humidity of nasal cavity and oral cavity, reach the purpose of continuously administering, avoided the nasal cavity mucous membrane dry, be fit for wearing under air drying's environment or various rhinitis, nasal cavity operation after.

Patent CN111387591A discloses a liquid mask patch, a preparation method and an application thereof, wherein the preparation raw materials comprise the following components: maltodextrin, sodium alginate, bacterial cellulose, hexanediol, xanthan gum, laurocapram, propylene glycol, cyclodextrin, hydrogenated coconut oil, glycerol, sodium hyaluronate, nicotinamide, vitamin C, traditional Chinese medicine extract, functional particles and purified water. The liquid mask paste can be quickly solidified to form a film on the inner side surface of the conventional mask, the facial skin is directly contacted with the liquid mask paste, the liquid mask paste can be matched with any conventional mask for use, and only the liquid mask paste needs to be directly coated or sprayed on the inner side surface of the conventional mask to be solidified to form a film during use.

The existing mask moisturizing products have insufficient moisturizing and skin care performances and use comfort, and if the film moisturizing substances cling to the surface of the skin for a long time, unsmooth skin breathing and uncomfortable feeling are easily caused, so that the mask which can protect the skin for a long time and moisturize the skin, has no obvious indentation and no damage after being worn for a long time, can reduce the pressure of the unit area of the nose and improve the sealing effect needs to be developed.

Disclosure of Invention

Aiming at the problems in the prior art, the invention provides moisturizing liquid and a mask moisturizing cotton batten.

Specifically, the present invention relates to the following aspects:

1. a moisturizing solution, comprising high molecular weight hyaluronic acid, low molecular weight hyaluronic acid, hydrolyzed hyaluronic acid, ectoin, caffeine, tartaric acid, chamomile extract, and ethylenediaminetetraacetic acid.

2. The moisturizing solution according to item 1, wherein the mass ratio of the high molecular weight hyaluronic acid is 0.05 to 0.2%, preferably 0.1 to 0.2%, the mass ratio of the low molecular weight hyaluronic acid is 0.05 to 0.2%, preferably 0.1 to 0.2%, and the mass ratio of the hydrolyzed hyaluronic acid is 0.05 to 0.1%, preferably 0.06 to 0.08%.

3. The moisturizing solution according to item 2, wherein the high molecular weight hyaluronic acid has a molecular weight of 1500-2000 kDa, the low molecular weight hyaluronic acid has a molecular weight of 50-100 kDa, and the hydrolyzed hyaluronic acid has a molecular weight of 8-10 kDa.

4. The moisturizing solution according to any one of items 1 to 3, wherein the mass ratio of the ectoin in the moisturizing solution is 0.1 to 0.3%, preferably 0.2 to 0.3%.

5. The moisturizing solution according to any one of claims 1 to 4, wherein the caffeine is contained in the moisturizing solution in a mass ratio of 0.05 to 0.2%, preferably 0.1 to 0.2%.

6. The moisturizing solution according to any one of claims 1 to 5, wherein the fruit acid is present in the moisturizing solution in a mass ratio of 0.3 to 0.6%, preferably 0.5 to 0.6%.

7. The moisturizing liquid according to any one of claims 1 to 6, wherein the weight ratio of the chamomile extract in the moisturizing liquid is 1 to 5%, preferably 2 to 3%.

8. The moisturizing solution according to any one of claims 1 to 7, wherein the ethylenediaminetetraacetic acid is present in the moisturizing solution in a mass ratio of 0.01 to 0.1%, preferably 0.04 to 0.06%.

9. The moisturizing solution according to any one of claims 1 to 8, further comprising 0.1 to 1% by mass of a preservative selected from one or more of citric acid, potassium sorbate and sodium benzoate.

10. A mask moisturizing cotton strip is prepared from the moisturizing liquid described in any one of the cotton strip spraying items 1-9. Compared with the prior art, the invention has the following beneficial effects: according to the mask moisturizing cotton batten provided by the invention, high molecular weight hyaluronic acid, low molecular weight hyaluronic acid and hydrolyzed hyaluronic acid are combined according to a certain proportion for compounding, the proportion of molecules is scientific, the macromolecular hyaluronic acid can form a film and can prevent water from evaporating, the molecular weight of the low molecular weight hyaluronic acid is relatively small, and the low molecular weight hyaluronic acid is compounded with low-concentration tartaric acid in a specific proportion, so that the use amount of the tartaric acid is reduced, the permeability can be improved, the transdermal absorption of hyaluronic acid, ectoine and chamomile extracts is promoted, the skin repair is accelerated, and the red swelling and allergic symptoms are relieved. According to the mask moisturizing cotton batten provided by the invention, the tartaric acid with extremely low concentration is a skin moisturizing agent, so that the cells on the surface layer of the skin contain sufficient moisture, and the fruit acid, the compound hyaluronic acid, the ectoin and the caffeine have a synergistic effect, so that a long-acting moisturizing barrier is formed on the nasal wing part, the long-acting moisturizing effect of the moisturizing cotton batten is obviously improved, a good moisturizing effect can be achieved after 8-hour testing, the long-acting moisturizing effect is better, and meanwhile, the red swelling caused by wearing the mask for a long time can be relieved.

According to the mask moisturizing cotton strip provided by the invention, due to the viscoelasticity and profiling performance of the compound hyaluronic acid, other large and small molecular components in the moisturizing liquid can be well dispersed in the structure of the compound hyaluronic acid, the stability and activity of the moisturizing liquid are improved, the moisturizing performance is improved, and the storage life of the mask moisturizing cotton strip is prolonged.

Detailed Description

The present invention is further illustrated by the following examples, which are intended to be purely exemplary of the invention and are not intended to be limiting.

Unless defined otherwise, technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. Although methods and materials similar or equivalent to those described herein can be used in experimental or practical applications, the materials and methods are described below. In case of conflict, the present specification, including definitions, will control, and the materials, methods, and examples are illustrative only and not intended to be limiting. The present invention is further illustrated by the following examples, which are not intended to limit the scope of the invention.

The invention provides a moisturizing liquid which comprises high molecular weight hyaluronic acid, low molecular weight hyaluronic acid, hydrolyzed hyaluronic acid, ectoin, caffeine, tartaric acid, chamomile extract and ethylenediaminetetraacetic acid.

Hyaluronic Acid (HA) is a natural mucopolysaccharide formed by alternating units of D-glucuronic Acid and N-acetylglucosamine in a linear chain. Hyaluronic acid shows a plurality of important physiological functions in organisms by virtue of unique molecular structures and physicochemical properties, and particularly has a special water retention effect, is a substance which is found to be the best in moisture retention in nature at present and is called ideal natural moisture retention factor (NMF). And the hyaluronic acid molecules are in a rigid spiral column shape in space, the inner side of the column generates strong water absorption due to the existence of a large number of hydroxyl groups, and on the other hand, due to the continuous directional arrangement of the hydroxyl groups, a hydrophobic region is formed on the molecular chain of the hyaluronic acid, so that the hyaluronic acid can form a three-dimensional network structure.

The hydrolyzed hyaluronic acid is a product prepared from hyaluronic acid by adopting a unique enzyme digestion technology, the molecular weight is about thousands to ten thousand, the molecular weight is the minimum, and the size is in a nanometer level. The hydrolyzed hyaluronic acid can rapidly permeate into skin, deeply supplement water of skin, repair damaged cells, and improve inflammation. In a specific embodiment, the mass ratio of the high molecular weight hyaluronic acid is 0.05 to 0.2%, for example, 0.05%, 0.06%, 0.07%, 0.08%, 0.09%, 0.1%, 0.11%, 0.12%, 0.13%, 0.14%, 0.15%, 0.16%, 0.17%, 0.18%, 0.19%, 0.2%, preferably 0.1 to 0.2%. The mass ratio of the low molecular weight hyaluronic acid is 0.05 to 0.2%, and may be, for example, 0.05%, 0.06%, 0.07%, 0.08%, 0.09%, 0.1%, 0.11%, 0.12%, 0.13%, 0.14%, 0.15%, 0.16%, 0.17%, 0.18%, 0.19%, 0.2%, and preferably 0.1 to 0.2%. The mass ratio of the hydrolyzed hyaluronic acid is 0.05 to 0.1%, and may be, for example, 0.05%, 0.06%, 0.07%, 0.08%, 0.09%, 0.1%, and preferably 0.06 to 0.08%.

The high molecular weight hyaluronic acid has a molecular weight of 1500kDa to 2000kDa, and may be 1500kDa, 1600kDa, 1700kDa, 1800kDa, 1900kDa or 2000kDa, for example. The low molecular weight hyaluronic acid has a molecular weight of 50kDa to 100kDa, and may be, for example, 50kDa, 60kDa, 70kDa, 80kDa, 90kDa or 100kDa. The molecular weight of the hydrolyzed hyaluronic acid is 8kDa to 10kDa, and may be 8kDa, 9kDa or 10kDa, for example.

Ectoine (Ectoine), also known as tetrahydropyrimidine (chemical name is 1,4,5,6-tetrahydro-2-methyl-4-pyrimidinecarboxylic acid, english name is 1,4,5,6-tetrahydro-2-methyl-4-pyrimidinecarboxylic acid), is a cyclic amino acid derivative, and can be used as a protective agent and a stabilizing agent of enzyme, nucleic acid, membrane and cells under extreme environments of high salt, thermal denaturation, drying, freezing and the like, so that the Ectoine has wide application prospects in the fields of enzyme preparation production, medicine, health food, cosmetic industry and the like.

In one specific embodiment, the weight ratio of ectoin in the moisturizing liquid is 0.1 to 0.3%, for example, 0.1%, 0.15%, 0.2%, 0.25%, 0.3%. Preferably 0.2 to 0.3%.

Caffeine, a xanthine alkaloid compound, is a central nervous stimulant that temporarily drives drowsiness and restores energy, and is used clinically to treat neurasthenia and coma resuscitation. In addition, the caffeine has high antioxidant activity, can promote metabolism, eliminate free radicals harmful to skin, prevent ultraviolet rays and promote the overall health of skin.

In one specific embodiment, the weight ratio of caffeine in the moisturizing liquid is 0.05 to 0.2%, for example, 0.05%, 0.1%, 0.15%, 0.2%, and preferably 0.1 to 0.2%.

Alpha Hydroxy Acid (AHA) is an effective component existing in various natural fruits or yoghurt as the name suggests, and is various organic acids extracted from fruits. Contains gluconic acid, malic acid, citrus acid, lactic acid, etc., and is called fruit acid because most of them are extracted from fruits. Among them, glycolic acid extracted from sugarcane is most widely used. Very low concentration of fruit acid, and has moisture keeping effect.

In one specific embodiment, the fruit acid may be contained in the moisturizing liquid in a mass ratio of 0.3 to 0.6%, for example, 0.3%, 0.4%, 0.5%, 0.6%, and preferably 0.5 to 0.6%.

The flos Matricariae Chamomillae extract is dry whole plant extract of Matricaria chamomilla L. Of Compositae, mainly contains flavone, saponin, polysaccharide, volatile oil, etc., and has antiinflammatory, fungi inhibiting, and spasmolytic effects; owing to their various medicinal effects, they have been used as cosmeceutical ingredients, and ointments, creams and lotions containing essential oil of chamomile are used in europe for the treatment of various skin diseases.

In one specific embodiment, the weight ratio of the chamomile extract in the moisturizing liquid is 1 to 5%, for example, 1%, 2%, 3%, 4%, 5%, preferably 2 to 3%.

In one moisturizing liquid, the Ethylene Diamine Tetraacetic Acid (EDTA) may be 0.01 to 0.1% by mass, for example, 0.01%, 0.02%, 0.03%, 0.04%, 0.05%, 0.06%, 0.07%, 0.08%, 0.09%, 0.1%, and preferably 0.04 to 0.06%.

The moisturizing liquid of the present invention may further include a preservative, and the preservative may be present in the moisturizing liquid in an amount of 0.1 to 1% by mass, for example, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, or 1%. The preservative is one or more than two of citric acid, potassium sorbate and sodium benzoate.

In a specific embodiment, the moisturizing liquid is composed of high molecular weight hyaluronic acid, low molecular weight hyaluronic acid, hydrolyzed hyaluronic acid, ectoin, caffeine, tartaric acid, chamomile extract, ethylenediaminetetraacetic acid, a preservative, and water. Further, the content of each substance may be as described above.

The invention also provides a mask moisturizing cotton strip which is prepared by spraying the moisturizing liquid on a cotton strip.

The moisturizing liquid provided by the invention combines hyaluronic acid, fruit acid, ectoin, chamomile extract and caffeine with different molecular weights according to a certain proportion for compounding, has better high-temperature stability and low irritation, and can be suitable for a moisturizing cotton strip of a mask; the moisturizing liquid has good moisturizing performance and long-acting moisturizing performance, the moisturizing rates of 6h and 12h are respectively as high as 80% and more than 70%, especially the moisturizing liquid prepared in examples 1-4, the moisturizing rate of 6h is more than 90%, and the moisturizing rate of 12h is also more than 80%, compared with the moisturizing liquid without hyaluronic acid or fruit acid, the moisturizing rate is improved by nearly 1 time, and especially the long-acting moisturizing performance is optimal; can increase the penetration and absorption of components such as ectoin in the moisturizing liquid, accelerate the fading of skin red and swollen, and is beneficial to relieving the skin red and swollen; the skin moisture content test, the percutaneous moisture loss and the indentation condition are ideal.

Examples

Example 1

(1) Fully dissolving and dispersing 0.2g of citric acid and 0.5g of potassium sorbate with purified water to obtain a solution 1;

(2) Dissolving 0.15g of caffeine, 0.25g of ectoin, 0.08g of hydrolyzed hyaluronic acid (9 kD), 0.15g of high molecular weight hyaluronic acid (1800 kDa) and 0.15g of low molecular weight hyaluronic acid (80 kDa) in purified water, and mixing to obtain solution 2;

(3) Adding the solution 1 into the solution 2, slowly, fully and uniformly stirring to obtain a solution 3;

(4) Adding the dissolving solution 3 into 0.05g of ethylenediamine tetraacetic acid, 2.5g of chamomile extract and 0.5g of tartaric acid while slowly stirring, supplementing water to 100ml, fully and uniformly stirring, and then preserving heat for 1.5 hours to obtain the moisturizing liquid.

The moisturizing cotton batten is prepared by using the prepared moisturizing liquid, and the specific steps are as follows:

(1) Manufacturing moisturizing cotton battens with uniform size strip structures;

(2) And slowly spraying the prepared moisturizing liquid to a cut and formed moisturizing cotton pressing strip with uniform size (the pressing strip is sterilized by ethylene oxide sterilizing gas) in an aseptic workshop environment, wherein the moisturizing cotton pressing strip rotates in the spraying process to fully ensure uniform absorption of the moisturizing liquid, and the moisturizing liquid is sprayed until the moisturizing liquid is not thrown out by rotation.

(2) And (3) sealing and aseptically packaging the humidified cotton sliver obtained in the step (2) to obtain the mask moisturizing cotton layering.

Examples 2 to 8

Examples 2 to 8 are different from example 1 in the amount of addition and molecular weight of high molecular weight hyaluronic acid, low molecular weight hyaluronic acid, hydrolyzed hyaluronic acid, and the amount of addition of fruit acid, and the other steps are the same. Specific conditions are shown in table 1.

Comparative example 1

Comparative example 1 differs from example 1 in that the other operation steps are the same without adding hydrolyzed hyaluronic acid.

Comparative example 2

Comparative example 2 is different from example 1 in that low molecular weight hyaluronic acid was not added and the other operation steps were the same.

Comparative example 3

Comparative example 3 is different from example 1 in that high molecular weight hyaluronic acid was not added and the other operation steps were the same.

Comparative example 4

Comparative example 4 differs from example 1 in that no fruit acid was added and the other operation steps were the same.

TABLE 1 different kinds of hyaluronic acid addition amounts and molecular weights of examples and comparative examples

Verification example

Verification example 1 high temperature Low temperature storage stability test

The moisturizing solutions prepared in examples 1 to 8 and comparative examples 1 to 4 were stored at-5 ℃ and 40 ℃ for 3 times in a circulating manner, each time for 24 hours, namely, after being stored at-5 ℃ for 24 hours, the moisturizing solutions were stored at room temperature for 24 hours, and then stored in a thermostat at 40 ℃ for 24 hours, and the storage was sequentially circulated for 3 times, and the moisturizing solutions were evaluated for stability by observing whether discoloration, delamination, caking, and the like were present, and the results were as shown in table 3 below.

TABLE 2 stability scoring criteria

TABLE 3 high and Low temperature storage stability test results

The moisturizing liquids prepared in the embodiments of the present invention have good stability, especially in embodiments 1 to 4, and the obtained moisturizing liquids have the highest stability, and have no color, delamination, and odor changes visible to the naked eye, and no caking phenomenon. In comparative examples 1 to 3, the stability of the moisturizing liquid was inferior to that of the examples, and delamination and blocking were observed, since the content, kind, etc. of hyaluronic acid were different from those of the examples.

Verification example 2 skin Patch test

12 groups of moisturizing samples were placed in 12 sample wells of the patch, and the sample wells were controlled with purified water, and had an area of 1cm × 1cm. The sample was attached to the arm flexor side of the volunteer for patch test. And observing the irritability of the sample tried by the volunteer, and respectively measuring the irritability of 5min, 30min, 2h, 12h and 24 h. The skin irritation score criteria are shown in table 4:

TABLE 4 skin irritation Scoring criteria

The skin irritation scores for the different samples are shown in table 5:

TABLE 5 Patch test results

	5min	30min	2h	12h	24h
						Example 1	0	0	0	0	0
Example 2	0	0	0	0	0
						Example 3	0	0	0	0	0
Example 4	0	0	0	0	0
						Example 5	0	0	0	0	0
Example 6	0	0	0	0	0
						Example 7	0	0	0	0	0
Example 8	0	0	0	0	1
						Comparative example 1	0	0	0	0	1
Comparative example 2	0	0	0	0	1
						Comparative example 3	0	0	0	0	1
Comparative example 4	0	0	0	0	1

The above experiments show that the moisturizers prepared in examples 1 to 7 of the present invention do not cause any skin reaction within 24 hours and are not irritating to the skin. Example 8 is slightly irritating to the skin due to the higher levels of fruit acids. Comparative examples 1-4 patch experiments 24h all had some irritation to the skin.

Proof example 3 product moisturizing Performance test study (in vitro weighing method)

1ml of the moisturizing solutions prepared in examples 1 to 8 and comparative examples 1 to 4 were weighed, respectively, and uniformly coated on 5cm × 5cm glass plates to which the microporous vent tapes were attached, respectively, and the glass plates were placed in a constant-temperature and constant-humidity dryer, and the mass of the glass plates after being placed for 6 hours and 12 hours was measured, respectively, and the moisturizing rates thereof were calculated. The moisture retention rate calculation formula is as follows: moisture retention/% = (M2-M0)/(M1-M0) × 100%.

Wherein M0 is the mass/g of the glass plate, M1 is the mass/g of the glass plate after sample loading, and M2 is the mass/g of the glass plate after being placed in a dryer for a plurality of hours.

The results of the in vitro weighing method for measuring the moisturizing rates of the moisturizing solutions prepared in examples 1 to 8 of the present invention and the moisturizing solutions prepared in comparative examples 1 to 4 are shown in the following table 6:

TABLE 6 test results of moisture retention Properties

	Moisture retention ratio (%) for 6h	Moisture retention rate (%) for 12h
			Example 1	93.4	84.6
Example 2	90.3	80.2
			Example 3	92.8	83.7
Example 4	91.8	82.5
			Example 5	83.1	70.8
Example 6	85.5	72.6
			Example 7	86.3	73.4
Example 8	86.4	73.6
			Comparative example 1	64.5	43.7
Comparative example 2	65.2	42.8
			Comparative example 3	63.4	41.0
Comparative example 4	68.7	45.0

The experiments show that the moisturizing liquid prepared in the embodiment of the invention has good moisturizing performance and long-acting moisturizing performance, the moisturizing rates of 6h and 12h are respectively as high as 80% and more than 70%, especially the moisturizing liquids prepared in the embodiments 1-4 have the moisturizing rates of 6h and 12h of more than 90% and more than 80%. The moisturizing rate and the moisturizing stability of the moisturizing liquids prepared in the comparative examples 1-4 are not ideal, and the results show that the compounded hyaluronic acid and the fruit acid in the moisturizing liquid are matched with each other, so that the moisturizing liquid has a synergistic effect, has an excellent moisturizing effect, can fundamentally relieve the problems of skin dryness and water loss, further improves the skin firmness and elasticity, and can rapidly promote the repair of skin redness and swelling.

Verification example 4 skin redness and swelling repair test

1. Skin red swelling mouse model

60 healthy mice are taken, half of the mice are female and half of the mice are depilated on the back of each group of mice, the depilated area is about 2cm x 2cm, the skin after depilation is cleaned by clean water, the mice are placed under an ultraviolet lamp (5 UVA lamp tubes of 40W and 1 UVB lamp tube of 36W) after 24 hours and are irradiated at the distance of 35cm from the mice, the irradiation time is 30min, and the continuous irradiation is carried out for 1 week.

2. Administration and assay

The mice are randomly divided into 12 groups, and under the environment with the same humidity and temperature, the mask moisturizing liquids prepared in examples 1-8 and comparative examples 1-4 are respectively and uniformly smeared on the red and swollen parts, and are smeared once every 4 hours for 7 days continuously. The days of disappearance of the red swelling were observed and recorded, and the results of the experiment are shown in Table 7.

TABLE 7 skin redness and swelling repair test results

	Skin red and swollen repair time
		Example 1	2d
Example 2	4d
		Example 3	2d
Example 4	3d
		Example 5	6d
Example 6	6d
		Example 7	6d
Example 8	6d
		Comparative example 1	The red swelling did not subside within the observation period
Comparative example 2	The red swelling did not subside within the observation period
		Comparative example 3	The red swelling did not subside within the observation period
Comparative example 4	Red in observation periodThe swelling did not subside

The moisturizing liquid prepared by the embodiment of the invention is compounded by hyaluronic acid and tartaric acid with different molecular weights, so that the penetration and absorption of components such as ectoin in the moisturizing liquid can be increased, the fading of red and swollen skin is accelerated, and the moisturizing liquid is favorable for quickly relieving the red and swollen skin. In the comparative example, the ratio of each component was not properly selected, and the red swelling subsided more slowly, and no red swelling subsided during the observation period.

Verification example 5 human wear test

65 volunteers, male and female halves, no skin problems such as allergy and pox on the face, were collected and divided into 13 groups of 5 persons each. In 12 groups of experimental groups, the mask moisturizing cotton strips prepared in examples 1-8 and comparative examples 1-4 are directly adhered to the inner side surface of a non-woven fabric mask, the non-woven fabric mask is worn for 8 hours every day and is continuously worn for 7 days, and a control group wears the non-woven fabric mask.

1. Skin moisture determination

Under the environment with the same humidity and temperature, the skin moisture content of the tested part of the test subject before wearing and the tested part of the test subject wearing the No. 7d are tested by a skin moisture tester, and the average value is obtained by repeating the measurement for 3 times. The results are shown in Table 8.

2. Determination of amount of moisture loss through skin

Transdermal water loss (TEWL) is closely related to the barrier function of skin and is also an important index for evaluating the barrier function of skin. The unit of measurement is g/m ² h, i.e., how many grams of water are lost per square meter area per hour. The transdermal water loss test reflects the water permeability barrier of the skin by measuring the water vapor pressure gradient at the skin surface, indicating the loss of water. When the barrier function is damaged, the TEWL value is increased; conversely, TEWL decreases, suggesting barrier repair.

The amount of percutaneous water loss at the test site was measured before wearing and after wearing a 7d skin water loss meter Tewameter TM300 (Courage + Khazaka Co., germany) under a ring mirror of the same humidity and temperature, and the measurement was repeated 3 times to obtain an average value. The results of this experiment are shown in table 8.

After the test is finished, the skin feeling of the wearer is investigated, and the skin indentation condition of the nose and wing parts of the wearer is observed, and the result is shown in table 8.

TABLE 8 skin moisture, transdermal water dispersion loss test results

The mask moisturizing cotton strips of examples 1 to 8 and comparative examples 1 to 4 are all performed according to set preparation parameters, according to the test results in table 8, the mask moisturizing cotton strips prepared in the examples can significantly improve the skin water content, reduce the transdermal water loss and repair the skin barrier, and no indentation phenomenon or no obvious indentation occurs to a wearer in the test period, while the mask moisturizing cotton strips prepared in the comparative examples 1 to 4 have better effects than the control group, but the skin water content test, the transdermal water loss and the indentation condition are still not ideal, which shows that the compounding of the high molecular weight hyaluronic acid, the low molecular weight hyaluronic acid, the hydrolyzed hyaluronic acid, the fruit acid and other components in the composition prepared in the invention produces the effects of synergy and long-term moisturizing, and the compounding of the hyaluronic acid and the fruit acid with different molecular weights can increase the permeation and absorption of the components in the moisturizing liquid, accelerate the repair of the skin barrier and avoid red swelling and indentation.

Claims

1. A moisturizing liquid is characterized in that the moisturizing liquid comprises high molecular weight hyaluronic acid, low molecular weight hyaluronic acid, hydrolyzed hyaluronic acid, ectoin, caffeine, tartaric acid, chamomile extract and ethylenediaminetetraacetic acid,

in the moisturizing liquid, the mass ratio of the high molecular weight hyaluronic acid is 0.05-0.2%, the mass ratio of the low molecular weight hyaluronic acid is 0.05-0.2%, the mass ratio of the hydrolyzed hyaluronic acid is 0.05-0.1%, the mass ratio of the fruit acid is 0.3-0.6%,

the molecular weight of the high molecular weight hyaluronic acid is 1500-2000 kDa, the molecular weight of the low molecular weight hyaluronic acid is 50-100 kDa, and the molecular weight of the hydrolyzed hyaluronic acid is 8-10 kDa.

2. The moisturizing solution according to claim 1, wherein the mass ratio of the high molecular weight hyaluronic acid to the low molecular weight hyaluronic acid is 0.1 to 0.2%, the mass ratio of the low molecular weight hyaluronic acid to the moisturizing solution is 0.1 to 0.2%, and the mass ratio of the hydrolyzed hyaluronic acid is 0.06 to 0.08%.

3. The moisturizing solution according to claim 1 or 2, wherein the ectoin is present in an amount of 0.1 to 0.3% by mass.

4. The moisturizing solution as claimed in any one of claims 1 to 3, wherein the ectoin is present in an amount of 0.2 to 0.3% by mass in the moisturizing solution.

5. The moisturizing solution according to any one of claims 1 to 3, wherein the caffeine is present in the moisturizing solution in a mass ratio of 0.05 to 0.2%.

6. The moisturizing solution according to claim 5, wherein the caffeine is present in the moisturizing solution in a mass ratio of 0.1 to 0.2%.

7. The moisturizing solution according to any one of claims 1 to 3, wherein the fruit acid is present in the moisturizing solution in a mass ratio of 0.5 to 0.6%.

8. The moisturizing solution according to any one of claims 1 to 3, wherein the weight ratio of the chamomile extract in the moisturizing solution is 1 to 5%.

9. The moisturizing solution according to claim 8, wherein the weight ratio of the chamomile extract in the moisturizing solution is 2 to 3%.

10. The moisturizing solution as claimed in any one of claims 1 to 3 and 9, wherein the weight ratio of ethylenediaminetetraacetic acid in the moisturizing solution is 0.01 to 0.1%.

11. The moisturizing solution as claimed in claim 10, wherein the ethylenediaminetetraacetic acid is present in the moisturizing solution in an amount of 0.04 to 0.06% by mass.

12. The moisturizing solution according to any one of claims 1 to 3, 9 and 11, further comprising 0.1 to 1% by mass of a preservative selected from one or more of citric acid, potassium sorbate and sodium benzoate.

13. A moisture-retaining tampon for a mask, prepared by spraying the moisture-retaining solution of any one of claims 1 to 12 onto a tampon.