CN111903429A - Phellinus igniarius planting method - Google Patents
Phellinus igniarius planting method Download PDFInfo
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- CN111903429A CN111903429A CN202010901824.1A CN202010901824A CN111903429A CN 111903429 A CN111903429 A CN 111903429A CN 202010901824 A CN202010901824 A CN 202010901824A CN 111903429 A CN111903429 A CN 111903429A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/50—Inoculation of spawn
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/60—Cultivation rooms; Equipment therefor
- A01G18/69—Arrangements for managing the environment, e.g. sprinklers
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Abstract
The invention belongs to the technical field of phellinus igniarius production, and particularly relates to a phellinus igniarius planting method which comprises the following steps of (1) preparing a mother culture medium of phellinus igniarius; (2) culturing the mother strain of phellinus igniarius; (3) preparing phellinus igniarius fungus bags; (4) culturing phellinus igniarius: (5) and (3) rejuvenating and culturing phellinus igniarius: (6) picking phellinus igniarius; the design of the components of the culture medium in the cultivation and planting process of the phellinus igniarius is adopted, so that the nutrient composition structure of the culture medium is obviously improved, long-term nutrient supply support is provided for the growth of the phellinus igniarius, in addition, the antibacterial property in the culture medium is obviously improved through the synergistic effect of the radix angelicae pubescentis, the cortex acanthopanacis and the silkworm excrement in the formula, and the combination of the design of the structure of the cultivation groove and the components of the culture medium is adopted, so that the phellinus igniarius has a shorter planting period, can be cultivated for many years, has stronger antibacterial property, and has.
Description
Technical Field
The invention belongs to the technical field of phellinus igniarius production, and particularly relates to a phellinus igniarius planting method.
Background
Phellinus genus is of Basidiomycotina, Hymenomycetes, Aphyllophorales, Hymenochaetaceae, Phellinus genus, and is a trade name of Phellinus igniarius and Phellinus linteus. The Shennong Bencao Jing and the Bencao gang mu have records on the medicinal efficacy of the phellinus igniarius, and the modern medical research shows that the phellinus igniarius has the obvious effects of resisting tumor, resisting oxidation, improving human immunity and the like, is a rare medicinal fungus with the first biological anti-tumor effect internationally acknowledged at present, and is widely applied to the industries of medicines, foods, daily chemicals, health care products and the like.
The applicant finds that the phellinus linteus in the phellinus linteus contains abundant total flavonoids and total triterpenes, which are the main components of the phellinus linteus for exerting the drug effect, but the artificially planted phellinus linteus often contains lower content of the total flavonoids and the total triterpenes, and cannot meet the market requirements. Meanwhile, the phellinus igniarius is difficult to plant, easy to contaminate and long in culture period, and can be harvested in at least three years. Therefore, the phellinus linteus in China is still obtained mainly by field picking at present, most of the existing perennial phellinus linteus cultivation is directly in contact with soil for cultivation, and various mixed bacteria in the soil are various, so that the survival rate of the phellinus linteus is influenced by the rapid propagation of various mixed bacteria after long-time cultivation, the diseases and the rot of the artificially-planted phellinus linteus in the second year begin to occur in large area due to the change of the environment, and no good solution exists at present.
Disclosure of Invention
The invention aims to provide a phellinus igniarius planting method which has a faster growth cycle, higher contents of total flavonoids and total triterpenoids and stronger antibacterial property.
In order to achieve the above object, an embodiment of the present invention provides a phellinus linteus planting method, including the following steps,
(1) preparing a phellinus igniarius mother culture medium:
the raw material ingredients comprise, by weight, 180 parts of sweet potatoes, 15-20 parts of jujube wood powder, 8-12 parts of corn flour, 7-11 parts of wheat flour, 25-28 parts of agar powder, 6-10 parts of glucose, 15-30 parts of silkworm excrement, 1-1.5 parts of radix angelicae pubescentis powder and 1000 parts of water; cutting sweet potato, decocting in water for 20 min, filtering, adding Zizyphi fructus wood powder, semen Maydis powder, semen Tritici Aestivi powder, agar powder, glucose, faeces Bombycis, and radix Angelicae Pubescentis powder, stirring, and autoclaving;
(2) culturing the mother strain of phellinus igniarius:
inoculating the culture medium obtained in the step (1), and culturing the inoculated culture medium in an environment at 28-30 ℃ until the mother strain of phellinus igniarius grows over a culture dish for later use;
(3) preparing a phellinus igniarius fungus bag:
the raw materials comprise, by weight, 100 parts of red date dregs, 20 parts of mulberry leaves, 10 parts of jujube wood powder, 15 parts of corncobs, 2 parts of gypsum, 2 parts of radix angelicae pubescentis powder, 5 parts of bean cakes, 10 parts of wheat bran and 20 parts of silkworm excrement; adding water into the above materials to make the humidity reach 55-60%, and adjusting pH to 7-7.6; pile fermentation for 3 days, and controlling the temperature not to exceed 60 ℃; placing the fermented culture medium into a fungus bag, and sterilizing at 125 deg.C and 0.6Mpa for 3 hr; after sterilization, when the temperature is reduced to 30 ℃, inoculating the phellinus igniarius mother seeds obtained in the step (2) into a phellinus igniarius fungus bag by using an aseptic inoculator; inoculating the phellinus igniarius fungus bags, wherein only one third of the area of one side of each phellinus igniarius fungus bag is inoculated, and marking is carried out through a marking pen;
(4) culturing phellinus igniarius:
placing the phellinus igniarius fungus bag obtained in the step (3) in a greenhouse with 65-75% of shading, placing the inoculation part upwards, maintaining the temperature at 20-28 ℃ and the humidity at 75-85% for culturing, cutting a small opening with the length of 1-3cm in the phellinus igniarius fungus bag inoculation part by using a knife after 25-30 days, and continuing culturing until the phellinus igniarius grows out of the fungus bag;
(5) and (3) rejuvenating and culturing phellinus igniarius:
digging a cultivation groove with the width of 70-120cm and the depth of 35-65cm in the greenhouse, and then spreading lime in the cultivation groove; next, laying an isolation film; then, a water pipe and a ventilation pipe are laid on the isolation film, the water pipe is provided with a water spraying hole, and the ventilation pipe is provided with a ventilation hole; laying aseptic isolation sponge on the water pipe and the ventilation pipe; laying a 10 cm-thick high-temperature steamed date wood section and a 10 cm-thick cortex acanthopanacis section on the upper part of the aseptic isolation sponge, and then spraying a mixture of sterilized cottonseed hulls and cow dung, wherein the length of the date wood section and the length of the cortex acanthopanacis section are 2-10 cm; uniformly mixing 50 parts of red date residues, 15 parts of mulberry leaves, 30 parts of jujube wood powder, 15 parts of corncobs, 2 parts of radix angelicae pubescentis powder, 5 parts of bean cakes, 10 parts of wheat bran and 20 parts of silkworm excrement, paving the mixture on the jujube wood section and the cortex acanthopanacis section after sterilization, transplanting the fungus bags with the phellinus igniarius grown out obtained in the step (4) into a cultivation groove, tearing off half of the fungus bags at the bottom in the cultivation process, then enabling the surfaces of the labeled inoculated phellinus igniarius mother seeds to face upwards, and burying the phellinus igniarius fungus bags in a culture medium for two-thirds cultivation; the upper part of the cultivation groove is covered with a heat preservation film; the distance between the cultivation grooves is 30-80 cm; controlling the temperature in the greenhouse to be 25 ℃ and the humidity to be 60%, opening the heat-insulating film for 1-2h every three days, and ventilating the greenhouse for 2 h; spraying water through a water pipe 2 times a day for 3min each time; introducing clean air 2-5 times per day through ventilation pipe, 3-10min each time, introducing air at a temperature 2-3 deg.C higher than room temperature;
(6) picking phellinus igniarius:
after the phellinus igniarius pileus turns yellow brown from light yellow, the pileus is tanned and picked; different cultivation times are selected according to the purpose of phellinus igniarius in the picking process.
Further, the step (1) of preparing the phellinus igniarius mother culture medium comprises the following steps: the raw materials comprise, by weight, 200 parts of sweet potatoes, 20 parts of jujube wood powder, 10 parts of corn flour, 11 parts of wheat flour, 25 parts of agar powder, 8 parts of glucose, 25 parts of silkworm excrement, 1.5 parts of radix angelicae pubescentis powder and 1000 parts of water.
Furthermore, the upper edge of the cultivation groove is 5-20cm higher than the phellinus igniarius fungus bag, so that film covering is facilitated, and influence on growth of phellinus igniarius in the film covering process is reduced.
Furthermore, one end of the bottom of the cultivation groove is higher and the other end is lower, and the cultivation groove is inclined at an angle of 13-20 degrees; set up the return water chamber in the one end of lower side for water in the water pipe is spraying the back, and unnecessary water can flow back down the return water chamber along the barrier film in the cultivation groove.
Furthermore, air in the ventilation pipe is introduced from the lower side of the bottom of the cultivation tank.
Further, after 24 months, the liquid nutrient solution is sprayed every 2 months.
Further, an isolation groove is arranged at the position of 10cm away from the edge of the cultivation groove, the width of the isolation groove is 3-5cm, the depth of the isolation groove is 5-10cm, and white lime is scattered in the isolation groove; the white lime is 3-5cm away from the upper edge of the isolation groove.
Furthermore, the isolation groove can accommodate the clamping of the film clamping rod, the film clamping rod is trapezoidal, and the film clamping rod is pressed on the film and is reversely buckled into the isolation groove to cover the film on the upper portion of the cultivation groove.
Further, in the step (5), the mixing proportion of the date wood segment, the cortex acanthopanacis segment, the cottonseed hulls and the cow dung is 10: 5: 6: 3.
further, in the step (5), the mixture of the date wood segments, the cortex acanthopanacis segments, the cottonseed hulls and the cow dung also comprises 3 parts of date leaves.
Compared with the prior art, the embodiment of the invention has the beneficial effects that: through the cooperation of the jujube wood powder, the radix angelicae pubescentis and silkworm excrement, the phellinus igniarius culture medium has a sterilization effect, the propagation of mold in the culture medium is well inhibited, and meanwhile, the phellinus igniarius acid rot is prevented; through the synergistic effect of the red date residues, silkworm excrement, mulberry leaves and the jujube wood powder, the phellinus igniarius can absorb cyclic adenosine monophosphate, polysaccharide, alcohols and other components in the red date residues more efficiently in the growth process, simultaneously provide sufficient ketone and polysaccharide components unique to the mulberry, and effectively improve the content of total flavonoids and total triterpenes in the finished phellinus igniarius; the culture period is shortened, the diseases are fewer, and the culture survival rate is greatly improved; the design of the whole structure of the cultivation tank ensures that the ventilation of the phellinus igniarius is better in the growth process, various germs contained in the soil are effectively isolated, the influence of the soil on the growth of the phellinus igniarius is reduced, the phellinus igniarius can be planted and grown for many years, and the problems of large-area bacterial contamination and acid rot outbreak caused by the deterioration of the planting environment can be solved; meanwhile, local resources are well utilized, the investment cost is lower, the suitable region is wide, the management cost is low, and the nutrition cost supply time is longer; tests show that the phellinus igniarius total flavone and total triterpenes produced by the planting method have higher content.
Detailed Description
In the description of the embodiments of the present invention, the meaning of "a plurality" means two or more unless otherwise specified. For example, a plurality of elements refers to two elements or more.
The term "and/or" herein is an association relationship describing an associated object, and means that there may be three relationships, for example, a display panel and/or a backlight, which may mean: there are three cases of a display panel alone, a display panel and a backlight at the same time, and a backlight alone. The symbol "/" herein denotes a relationship in which the associated object is or, for example, input/output denotes input or output.
In the embodiments of the present invention, words such as "exemplary" or "for example" are used to mean serving as examples, illustrations or descriptions. Any embodiment or design described as "exemplary" or "e.g.," an embodiment of the present invention is not necessarily to be construed as preferred or advantageous over other embodiments or designs. Rather, use of the word "exemplary" or "such as" is intended to present concepts related in a concrete fashion.
Embodiment 1 a phellinus igniarius planting method, comprising the following steps of, (1) preparing a mother culture medium of phellinus igniarius: the raw materials comprise, by weight, 180kg of sweet potatoes, 15kg of jujube wood powder, 8kg of corn powder, 7kg of wheat powder, 25kg of agar powder, 6kg of glucose, 15kg of silkworm excrement, 1kg of radix angelicae pubescentis powder and 1000kg of water; chopping sweet potato, decocting in water for 20 min, filtering, adding Zizyphi fructus wood powder, semen Maydis powder, semen Tritici Aestivi powder, agar powder, glucose, faeces Bombycis, and radix Angelicae Pubescentis powder, stirring, and autoclaving. The addition of the jujube wood powder, the silkworm excrement and the radix angelicae pubescentis powder effectively improves the inhibition effect of a culture medium on mold, increases the resistance of a phellinus igniarius mother seed to germs such as green mold and the like, simultaneously shortens the culture period of the phellinus igniarius mother seed, enables the phellinus igniarius mother seed to grow better, and enables the phellinus igniarius mother seed to fully absorb beneficial ingredients of mulberry in the silkworm excrement to ensure that the later-period beneficial ingredients of the phellinus igniarius reach the standard of.
The radix angelicae pubescentis powder contains the components of saxifragin, sitosterol, bergapten, xanthotoxin and the like, and can effectively kill or inhibit the growth of pseudomonas aeruginosa and neurospora in phellinus igniarius culture. The silkworm excrement contains beta-sitosterol, lupulin, ammonia salt, ammonium salt, antibiotics and the like, so that the silkworm excrement can effectively inhibit the growth of various ova and reduce insect pests, and the silkworm excrement and the radix angelicae pubescentis powder have a synergistic effect to improve the antibacterial property of the silkworm excrement. In addition, silkworm excrement contains abundant microelements, and can promote the growth of phellinus igniarius mother seeds.
(2) Culturing the mother strain of phellinus igniarius: inoculating the culture medium obtained in the step (1), and culturing the inoculated culture medium in a sterile environment at 28-30 ℃ until the mother strain of phellinus igniarius grows over a culture dish for later use.
(3) Preparing a phellinus igniarius fungus bag: the raw materials comprise, by weight, 100kg of red date dregs, 20kg of mulberry leaves, 10 parts of jujube wood powder, 15kg of corncobs, 2kg of gypsum, 2kg of radix angelicae pubescentis powder, 5kg of bean cakes, 10kg of wheat bran and 20kg of silkworm excrement; adding water into the ingredients to make the humidity reach 60%, and adjusting the PH to 7; pile fermentation for 3 days, and controlling the temperature not to exceed 60 ℃; the bactericidal components in the radix angelicae pubescentis powder are thoroughly released through pile fermentation, and the antibacterial property of the whole culture medium is improved. Placing the fermented culture medium into a fungus bag, and sterilizing at 125 deg.C and 0.6Mpa for 3 hr; after sterilization, when the temperature is reduced to 30 ℃, inoculating the phellinus igniarius mother seeds obtained in the step (2) into a phellinus igniarius fungus bag by using an aseptic inoculator; inoculating the phellinus igniarius fungus bags, wherein only one third of the area of one side of each phellinus igniarius fungus bag is inoculated, and marking is carried out through a marking pen; the production of jujube trees around the elm forest has the advantages that the output of red jujubes is in the front of China, and a plurality of red jujube manufacturers exist, so that the red jujube residues are used as cultivation raw materials, on one hand, the phellinus igniarius can repeatedly absorb the beneficial ingredients such as polysaccharide, cyclic adenosine monophosphate and the like in the red jujube residues in the growth process, and meanwhile, the reutilization of wastes is promoted. In addition, silkworm excrement, mulberry leaves and the like provide sufficient substances such as lipid, polysaccharide, triterpene and the like which are unique to mulberry trees for the mulberry trees, so that the content of total triterpene and brass in the later stage of the phellinus igniarius is increased, the resistance of a culture medium to infectious microbes such as green enzyme and neurospora is effectively improved by combining the silkworm excrement and the radix angelicae pubescentis powder, and the survival rate and the environmental adaptability of the phellinus igniarius are improved.
(4) Culturing phellinus igniarius: placing the phellinus igniarius fungus bags obtained in the step (3) in a greenhouse with 75% of shading, placing an inoculation part upwards on a phellinus igniarius culture frame of the greenhouse, wherein the phellinus igniarius culture frame is arranged in a multilayer mode, and therefore space utilization rate is improved; maintaining the temperature at 20-28 deg.C and humidity at 75-85%, ventilating 2 times per day, and spraying water 3 times; cutting a 1-3cm small opening at the inoculated part of the Phellinus igniarius fungus bag by a knife 25 days later, and continuing culturing until Phellinus igniarius grows out of the fungus bag. The phellinus igniarius is cultivated in the greenhouse provided with the cultivation frame for centralized cultivation, so that the space utilization rate is improved, centralized management is realized, and the management cost is reduced.
(5) And (3) rejuvenating and culturing phellinus igniarius: digging a cultivation groove with the width of 90cm and the depth of 50cm in the greenhouse, and then spreading lime in the cultivation groove; next, laying a layer of PE isolation film; the culture medium is isolated from the soil by the PE isolating film, so that the culture medium is effectively prevented from being polluted by mixed bacteria in the soil, and in the long-term cultivation process, nutrient substances and water are permeated into the soil to cause the loss of the nutrient substances and the water, meanwhile, the cultivation cost is reduced, and the water retention is improved; then, a water pipe and a ventilation pipe are laid on the isolation film, the water pipe is provided with a water spraying hole, and the ventilation pipe is provided with a ventilation hole; the water pipe is used for spraying water or nutrient substances from the bottom of the culture medium, so that the water consumption is reduced, the humidity in the culture medium is effectively improved, and the growth of phellinus igniarius is promoted; the ventilation pipe is used for letting in air from the bottom, increases the inside circulation of air of culture medium, and the production of more effectual prevention mould increases the phellinus igniarius oxygen simultaneously and supplies and promote the growth. Sterile isolation sponge is laid on the water pipe and the ventilation pipe, the water retention of the culture medium is effectively improved by the sterile isolation sponge, nutrient substances are locked to prevent loss, and the sterile isolation sponge has good air permeability and promotes bottom air circulation; and then laying a 10 cm-thick high-temperature steamed jujube wood section and a 10 cm-thick cortex acanthopanacis section on the upper part of the aseptic isolation sponge, and then spraying a mixture of sterilized cottonseed hulls and cow dung, wherein the lengths of the jujube wood section and the cortex acanthopanacis section are 5 cm. The jujube wood section and the cortex acanthopanacis section are rich in yield, easy to obtain and low in cost, the jujube wood section and the cortex acanthopanacis section subjected to high-temperature cooking slowly corrode in a humid environment, on one hand, the cortex acanthopanacis has a certain sterilization effect and can prevent germs, on the other hand, the jujube wood is relatively hard and can provide nutrient release for a long time and improve the durability of a culture medium, and the jujube wood has a good supporting effect at the bottom for a long time and promotes the permeability with an upper culture medium, and the combination of the jujube wood section and the cortex acanthopanacis section can provide multiple nutritional ingredients such as N, P, K, polysaccharide organic matters and the like needed for the growth of phellinus igniarius for a long time.
Then, (5) 50kg of red date residues, 15kg of mulberry leaves, 30kg of jujube wood powder, 15kg of corncobs, 2kg of radix angelicae pubescentis powder, 5kg of bean cakes, 10kg of wheat bran and 20kg of silkworm excrement are uniformly mixed, sterilized and then paved on layers of jujube wood segments and cortex acanthopanacis segments to form an upper layer culture medium, then the phellinus igniarius fungus bags growing out of phellinus igniarius obtained in the step (4) are transplanted into a cultivation groove, half of fungus bags at the bottom are torn off in the cultivation process, the culture medium in the cultivation groove is used for fully supplying nutrition, and the water retention property of the phellinus igniarius fungus bags is improved; then, the surface of the mother strain of the marked inoculated phellinus igniarius is upward and exposed outside, the culture medium is buried in phellinus igniarius fungus bags for two thirds of cultivation, and after the cultivation is finished, a heat preservation film is covered on the upper part of the cultivation groove, so that the heat preservation performance and the water retention performance are further improved; the phellinus igniarius fungus bags are arranged at intervals of 60cm between the cultivation grooves, so that management is facilitated, and space is reasonably utilized; controlling the temperature in the greenhouse to be between 25 ℃ and the humidity to be 60%, opening the heat-preservation film for 2 hours every three days 2 months before cultivation, and ventilating the greenhouse for 2 hours; spraying water from the bottom for 2 times every day, 3min each time, and spraying water from the upper part for 1 time; introducing clean air 2-5 times per day through ventilation pipe, 3-10min each time, introducing air at a temperature 2-3 deg.C higher than room temperature; after 2 months of cultivation, water is sprayed from the bottom 2 times, water is sprayed from the upper part once, the bottom is ventilated for 2 times, 3min each time, and the greenhouse is ventilated for 1 time for 2 days, so that air pollution is reduced.
(6) Picking phellinus igniarius:
after the phellinus igniarius pileus turns yellow brown from light yellow, the pileus is tanned and picked; different cultivation times are selected according to the purpose of phellinus igniarius in the picking process. Since high-quality phellinus igniarius needs to grow for many years to meet the content requirement of beneficial substances, phellinus igniarius of different growth years can be selected to be picked according to different requirements.
Through the cooperation of the jujube wood powder, the radix angelicae pubescentis and silkworm excrement, the phellinus igniarius culture medium has a sterilization effect, the propagation of mold in the culture medium is well inhibited, and meanwhile, the phellinus igniarius acid rot is prevented; through the synergistic effect of the red date residues, silkworm excrement, mulberry leaves and the jujube wood powder, the phellinus igniarius can absorb cyclic adenosine monophosphate, polysaccharide, alcohols and other components in the red date residues more efficiently in the growth process, simultaneously provide sufficient ketone and polysaccharide components unique to the mulberry, and effectively improve the content of total flavonoids and total triterpenes in the finished phellinus igniarius; the culture period is shortened, the diseases are fewer, and the culture survival rate is greatly improved; the design of the whole structure of the cultivation tank ensures that the ventilation of the phellinus igniarius is better in the growth process, various germs contained in the soil are effectively isolated, the influence of the soil on the growth of the phellinus igniarius is reduced, the phellinus igniarius can be planted and grown for many years, and the problems of large-area bacterial contamination and acid rot outbreak caused by the deterioration of the planting environment can be solved; meanwhile, local resources are well utilized, the investment cost is lower, the method is suitable for wide regions and low in management cost, and the growth nutrition requirement of phellinus igniarius within 5 years is effectively guaranteed; tests show that the phellinus igniarius total flavone and total triterpenes produced by the planting method have higher content.
In one embodiment of the present invention, the step (1) of preparing the culture medium of phellinus igniarius mother strain comprises: the raw materials comprise 200kg of sweet potato, 20kg of jujube wood powder, 10kg of corn powder, 11kg of wheat powder, 25kg of agar powder, 8kg of glucose, 25kg of silkworm excrement, 1.5kg of radix angelicae pubescentis powder and 1000kg of water.
Illustratively, the upper edge of the cultivation groove is 5cm, 10cm or 20cm higher than the phellinus igniarius fungus bag, so that the film covering is facilitated, the influence on the growth of the phellinus igniarius in the film covering process is reduced, the heat preservation performance is improved, and the growth speed of the phellinus igniarius is further improved.
Preferentially, one end of the bottom of the cultivation groove is higher, the other end of the bottom of the cultivation groove is lower, and the cultivation groove is inclined at 13 degrees; set up the return water chamber in the one end of lower side for water in the water pipe is spraying the back, and unnecessary water can flow back down the return water chamber along the barrier film in the cultivation groove. Effectively reducing the formation of cultivation tank bottom ponding on the one hand, preventing that mould from breeding, on the other hand converges the return water intracavity again through with unnecessary water system to water economy on the one hand, the existence in a plurality of return water chambeies in the on the other hand big-arch shelter can show the humidity that increases in the big-arch shelter, reduces the water spray number of times, improves phellinus igniarius growth speed.
Illustratively, air in the ventilation pipe is introduced from the lower side of the bottom of the cultivation tank, so that the ventilation effect is improved, the air can penetrate through the aseptic isolation sponge, the date wood section and the cortex acanthopanacis section layer as far as possible and enter the upper layer culture medium, the occurrence of rot is prevented, the growth of hyphae is promoted, and the growth speed of phellinus igniarius is increased.
Illustratively, the liquid nutrient solution is sprayed every 2 months after 24 months.
In one embodiment of the invention, an isolation groove is further arranged at the edge 10cm of the cultivation groove, the width of the isolation groove is 3-5cm, the depth of the isolation groove is 5-10cm, and white lime is scattered in the isolation groove; the white lime is 3-5cm away from the upper edge of the isolation groove. Thereby be used for forming certain median between cultivation groove and cultivation groove, be convenient for on the one hand cut apart the management, on the other hand can form the isolation between each cultivation groove, after the disease of phellinus igniarius in a cultivation groove, can play certain isolation effect.
On the basis of the technical scheme, the isolation groove can contain the clamping of the film clamping rods, the film clamping rods are trapezoidal, and the film clamping rods are pressed on the films and are reversely buckled into the isolation groove to cover the films on the upper portion of the cultivation groove. After the film covers, for the fixed of more convenient realization film to utilize a trapezoidal film joint pole, press the film in the isolation tank, because the isolation tank still is provided with the lime, consequently in the cultivation inslot of cover film, also formed the round median in the periphery in other words, thereby the film is fixed more conveniently, also is favorable to the isolation management of isolation tank and isolation tank simultaneously more.
Illustratively, in the step (5), the mixing ratio of the date wood segment, the cortex acanthopanacis segment, the cottonseed hull and the cow dung is 10: 5: 6: 3. therefore, the nutrition collocation of the jujube wood section and the cortex acanthopanacis section layer is more reasonable, a sterilization layer which has better permeability and can provide long-term nutrient supply is formed at the bottom of the upper culture medium, certain sterilization can be realized, the growth of mold is inhibited, the growth of phellinus igniarius is better promoted, and the survival rate is improved. The jujube wood segments are hard, can provide support for a long time and keep permeability.
On the basis of the scheme, in the step (5), 3kg of jujube leaves are further included in the mixture of the jujube wood segments, the cortex acanthopanacis segments, the cottonseed hulls and the cow dung. N, P, K which is weighed and various organic matters and trace elements which are required by growth are provided for the growth of the phellinus igniarius at the early stage of the cattle manure, the cottonseed hulls and the jujube leaves, nutrition is provided for the phellinus igniarius at the later stage of the growth by corrosion of cortex acanthopanacis and jujube wood segments, the nutrition supply is more durable, polysaccharides, cyclic adenosine monophosphate and phenolic acids in the jujube leaves and the jujube wood can also provide sufficient nutritional ingredients for accumulation of total flavonoids and total triterpenes in the growth process of the phellinus igniarius, and the content of the total flavonoids and the total triterpenes in the phellinus igniarius is improved.
In a preferred embodiment, the phellinus igniarius planting method comprises the following steps of (1) preparing a mother culture medium of phellinus igniarius: the raw materials comprise, by weight, 200kg of sweet potatoes, 18kg of jujube wood powder, 12kg of corn powder, 11kg of wheat powder, 28kg of agar powder, 10kg of glucose, 30kg of silkworm excrement, 1.5kg of radix angelicae pubescentis powder and 1000kg of water; chopping sweet potato, decocting in water for 20 min, filtering, adding Zizyphi fructus wood powder, semen Maydis powder, semen Tritici Aestivi powder, agar powder, glucose, faeces Bombycis, and radix Angelicae Pubescentis powder, stirring, and autoclaving. The addition of the jujube wood powder, the silkworm excrement and the radix angelicae pubescentis powder effectively improves the inhibition effect of a culture medium on mold, increases the resistance of a phellinus igniarius mother seed to germs such as green mold and the like, simultaneously shortens the culture period of the phellinus igniarius mother seed, enables the phellinus igniarius mother seed to grow better, and enables the phellinus igniarius mother seed to fully absorb beneficial ingredients of mulberry in the silkworm excrement to ensure that the later-period beneficial ingredients of the phellinus igniarius reach the standard of.
The radix angelicae pubescentis powder contains the components of saxifragin, sitosterol, bergapten, xanthotoxin and the like, and can effectively kill or inhibit the growth of pseudomonas aeruginosa and neurospora in phellinus igniarius culture. The silkworm excrement contains beta-sitosterol, lupulin, ammonia salt, ammonium salt, antibiotics and the like, so that the silkworm excrement can effectively inhibit the growth of various ova and reduce insect pests, and the silkworm excrement and the radix angelicae pubescentis powder have a synergistic effect to improve the antibacterial property of the silkworm excrement. In addition, silkworm excrement contains abundant microelements, and can promote the growth of phellinus igniarius mother seeds.
(2) Culturing the mother strain of phellinus igniarius: inoculating the culture medium obtained in the step (1), and culturing the inoculated culture medium in a sterile environment at 28-30 ℃ until the mother strain of phellinus igniarius grows over a culture dish for later use.
(3) Preparing a phellinus igniarius fungus bag: the raw materials comprise 100kg of red date dregs, 20kg of mulberry leaves, 10kg of jujube wood powder, 15kg of corncobs, 2kg of gypsum, 2kg of radix angelicae pubescentis powder, 5kg of bean cakes, 10kg of wheat bran and 20kg of silkworm excrement; adding water into the ingredients to make the humidity reach 55%, and adjusting the PH to 7; pile fermentation for 3 days, and controlling the temperature not to exceed 60 ℃; placing the fermented culture medium into a fungus bag, and sterilizing at 125 deg.C and 0.6Mpa for 3 hr; after sterilization, when the temperature is reduced to 30 ℃, inoculating the phellinus igniarius mother seeds obtained in the step (2) into a phellinus igniarius fungus bag by using an aseptic inoculator; inoculating the phellinus igniarius fungus bags, wherein only one third of the area of one side of each phellinus igniarius fungus bag is inoculated, and marking is carried out through a marking pen; the production of jujube trees around the elm forest has the advantages that the output of red jujubes is in the front of China, and a plurality of red jujube manufacturers exist, so that the red jujube residues are used as cultivation raw materials, on one hand, the phellinus igniarius can repeatedly absorb the beneficial ingredients such as polysaccharide, cyclic adenosine monophosphate and the like in the red jujube residues in the growth process, and meanwhile, the reutilization of wastes is promoted. In addition, silkworm excrement, mulberry leaves and the like provide sufficient substances such as lipid, polysaccharide, triterpene and the like which are unique to mulberry trees for the mulberry trees, so that the content of total triterpene and brass in the later stage of the phellinus igniarius is increased, the resistance of a culture medium to infectious microbes such as green enzyme and neurospora is effectively improved by combining the silkworm excrement and the radix angelicae pubescentis powder, and the survival rate and the environmental adaptability of the phellinus igniarius are improved.
(4) Culturing phellinus igniarius: placing the phellinus igniarius fungus bags obtained in the step (3) in a greenhouse with 70% of shading, placing an inoculation part upwards on a phellinus igniarius culture frame of the greenhouse, wherein the phellinus igniarius culture frame is arranged in a multilayer mode, and therefore space utilization rate is improved; maintaining the temperature at 27 deg.C and humidity at 85%, ventilating 3 times per day, and spraying water 4 times; after 30 days, a small opening with the length of 3cm is cut at the inoculation part of the phellinus igniarius fungus bag by a knife, and the culture is continued until the phellinus igniarius grows out of the fungus bag. The phellinus igniarius is cultivated in the greenhouse provided with the cultivation frame for centralized cultivation, so that the space utilization rate is improved, centralized management is realized, and the management cost is reduced.
(5) And (3) rejuvenating and culturing phellinus igniarius: digging a cultivation groove with the width of 120cm and the depth of 65cm in the greenhouse, and then spreading lime in the cultivation groove; next, laying a layer of PE isolation film; the culture medium is isolated from the soil by the PE isolating film, so that the culture medium is effectively prevented from being polluted by mixed bacteria in the soil, and in the long-term cultivation process, nutrient substances and water are permeated into the soil to cause the loss of the nutrient substances and the water, meanwhile, the cultivation cost is reduced, and the water retention is improved; one end of the bottom of the cultivation groove is higher and the other end is lower, and the cultivation groove is inclined at an angle of 20 degrees; set up the return water chamber in the one end of lower side for water in the water pipe is spraying the back, and unnecessary water can flow back down the return water chamber along the barrier film in the cultivation groove. Effectively reducing the formation of cultivation tank bottom ponding on the one hand, preventing that mould from breeding, on the other hand converges the return water intracavity again through with unnecessary water system to water economy on the one hand, the existence in a plurality of return water chambeies in the on the other hand big-arch shelter can show the humidity that increases in the big-arch shelter, reduces the water spray number of times, improves phellinus igniarius growth speed. Then, a water pipe and a ventilation pipe are laid on the isolation film, the water pipe is provided with a water spraying hole, and the ventilation pipe is provided with a ventilation hole; the air in the ventilation pipe is introduced from the lower side of the bottom of the cultivation tank. The water pipe is used for spraying water or nutrient substances from the bottom of the culture medium, so that the water consumption is reduced, the humidity in the culture medium is effectively improved, and the growth of phellinus igniarius is promoted; the ventilation pipe is used for letting in air from the bottom, increases the inside circulation of air of culture medium, and the production of more effectual prevention mould increases the phellinus igniarius oxygen simultaneously and supplies and promote the growth. Sterile isolation sponge is laid on the water pipe and the ventilation pipe, the water retention of the culture medium is effectively improved by the sterile isolation sponge, nutrient substances are locked to prevent loss, and the sterile isolation sponge has good air permeability and promotes bottom air circulation; and then laying a 10 cm-thick high-temperature steamed jujube wood section and a 10 cm-thick cortex acanthopanacis section on the upper part of the aseptic isolation sponge, and then spraying a mixture of sterilized cottonseed hulls, cow dung and the cortex acanthopanacis section, wherein the lengths of the jujube wood section and the cortex acanthopanacis section are 5 cm. The mixing proportion of the jujube wood segments, the cortex acanthopanacis segments, the cottonseed hulls, the cow dung and the jujube leaves is 10 parts by weight: 5: 6: 3: 3. the jujube wood section and the cortex acanthopanacis section are rich in yield, easy to obtain and low in cost, the jujube wood section and the cortex acanthopanacis section subjected to high-temperature cooking slowly corrode in a humid environment, on one hand, the cortex acanthopanacis has a certain sterilization effect and can prevent germs, on the other hand, the jujube wood is relatively hard and can provide nutrient release for a long time and improve the durability of a culture medium, and the jujube wood has a good supporting effect at the bottom for a long time and promotes the permeability with an upper culture medium, and the combination of the jujube wood section and the cortex acanthopanacis section can provide multiple nutritional ingredients such as N, P, K, polysaccharide organic matters and the like needed for the growth of phellinus igniarius for a long time.
Then, 50kg of red date residues, 15kg of mulberry leaves, 30kg of jujube wood powder, 15kg of corncobs, 2kg of radix angelicae pubescentis powder, 5kg of bean cakes, 10kg of wheat bran and 20kg of silkworm excrement are uniformly mixed, sterilized and then paved on the jujube wood segment layer and the cortex acanthopanacis segment layer to form an upper layer culture medium, then the phellinus igniarius fungus bags growing out of phellinus igniarius obtained in the step (4) are transplanted into a cultivation groove, half fungus bags at the bottom are torn off in the cultivation process, the culture medium in the cultivation groove is used for realizing full nutrition supply, and the water retention property of the phellinus igniarius fungus bags is increased through the reserved fungus bags; then, the surface of the mother strain of the marked inoculated phellinus igniarius is upward and exposed outside, the culture medium is buried in phellinus igniarius fungus bags for two thirds of cultivation, and after the cultivation is finished, a heat preservation film is covered on the upper part of the cultivation groove, so that the heat preservation performance and the water retention performance are further improved; the phellinus igniarius fungus bags are arranged at intervals of 80cm between the cultivation grooves, so that management is facilitated, and space is reasonably utilized; the upper edge of the cultivation groove is 20cm higher than the phellinus igniarius fungus bag, so that film covering is facilitated, and influence on growth of phellinus igniarius in the film covering process is reduced. An isolation groove is further arranged at the position 10cm away from the edge of the cultivation groove, the width of the isolation groove is 5cm, the depth of the isolation groove is 5-10cm, and white lime is scattered in the isolation groove; the white lime is 3 centimeters away from the upper edge of the isolation groove. The isolation groove can contain the clamping of the film clamping rod, the film clamping rod is trapezoidal, and the film clamping rod is pressed on the film and is reversely buckled into the isolation groove to cover the film on the upper portion of the cultivation groove. Controlling the temperature in the greenhouse to be between 25 ℃ and the humidity to be 60%, opening the heat-preservation film for 2 hours every three days 2 months before cultivation, and ventilating the greenhouse for 2 hours; spraying water from the bottom for 2 times every day, 3min each time, and spraying water from the upper part for 1 time; introducing clean air 2-5 times per day through ventilation pipe, 3-10min each time, introducing air at a temperature 2-3 deg.C higher than room temperature; after 2 months of cultivation, water is sprayed from the bottom 2 times, water is sprayed from the upper part once, the bottom is ventilated for 2 times, each time is 30min, and the greenhouse is ventilated for 1 time for 2 days, so that air pollution is reduced.
(6) Picking phellinus igniarius:
after the phellinus igniarius pileus turns yellow brown from light yellow, the pileus is tanned and picked; different cultivation times are selected according to the purpose of phellinus igniarius in the picking process. Since high-quality phellinus igniarius needs to grow for many years to meet the content requirement of beneficial substances, phellinus igniarius of different growth years can be selected to be picked according to different requirements.
The culture medium disclosed in the prior art is prepared by taking the culture medium formula disclosed in the prior art, wherein the culture medium formula comprises 77% of wood chips, 15% of bran, 5% of corn flour, 1% of five-dimensional glucose, 1% of potassium dihydrogen phosphate and 1% of gypsum, and silkworm excrement, radix angelicae pubescentis, cortex acanthopanacis and jujube wood powder are not added in the culture medium of the embodiment 1, the optimal embodiment and the culture medium of the application. The water content of the compost is 65%, and the compost is used as a reference group for testing. Similarly, 100 bags of culture medium were used as test material to examine the number of Phellinus linteus, the number of survivals, the time for full growth of hyphae, the time for forming fruiting bodies, the production time, the weight of harvested Phellinus linteus, and the weight of total flavonoids and total triterpenes contained in Phellinus linteus of the same weight.
The test results are described in the following table one:
through the test results, the formula of the culture medium has certain antibacterial property compared with the culture medium without experimental objects in the prior art, and the silkworm excrement, the cortex acanthopanacis and the radix angelicae pubescentis are added independently, so that the number of the infected bacteria is relatively low, but the three materials are matched to generate mutual synergistic effect, the antibacterial property is obviously superior to that of the single use, and meanwhile, the whole phellinus igniarius has better growth vigor, the culture medium has certain antibacterial property, and the antibacterial property of the phellinus igniarius is also increased; in addition, through the combination of the formula, the growth vigor of phellinus igniarius hyphae is better, the whole picking period of phellinus igniarius is shortened, and compared with the yield of a single fungus bag in the prior art, the yield of phellinus igniarius hyphae is also obviously improved. The content of total triterpenes in phellinus linteus with the same quality is measured by a vanillin-glacial acetic acid-perchloric acid spectrophotometry method, and the content of total flavonoids is measured by an aluminum nitrate-sodium nitrite chromogenic system ultraviolet spectrophotometry method, as can be seen from table 1, the addition of silkworm excrement has great influence on the content of total triterpenes and total flavonoids in phellinus linteus, and the content of the silkworm excrement is obviously reduced when the silkworm excrement is not added, meanwhile, the jujube wood powder has a certain promotion effect on the content of the jujube wood powder, and analysis shows that the silkworm excrement possibly contains beta-sitosterol, lupulin, ammonia salt, ammonium salt, antibiotics and the like, thereby effectively inhibiting the growth of various ova and reducing insect pests, and in addition, the rich trace elements contained in the silkworm excrement can effectively promote the growth of phellinus igniarius, meanwhile, the synergistic effect of the mulberry leaves and the jujube wood provides nutrient substances of the Chongzi for the phellinus igniarius to promote the synthesis of the total triterpenes and the total flavone content. In addition, the cultivation groove designed by the application is utilized, so that the success rate of cultivation is further improved, the survival rate of phellinus igniarius is higher, the bacterial contamination rate is lower, and the yield is higher. Particularly, after 15 months, the phellinus igniarius cultured in the fungus bag has poor growth vigor due to the consumption of nutrient substances, the phellinus igniarius in the cultivation tank still has good growth vigor, and the phellinus igniarius cultured in the fungus bag is supplied with water and air at the bottom to increase the air circulation at the bottom, so that the growth of hyphae in a culture medium is effectively promoted, sufficient nutrition is provided, the content of total triterpenes and total flavonoids is promoted to be improved, and the production period is shortened. The phellinus igniarius planting method described in the present application produces phellinus igniarius, both in terms of yield, production cycle and total triterpene and total flavone content, which are different from those of the prior art. Compared with the existing phellinus igniarius cultivation, the cultivation period is shortened, the product quality is improved, the content of total triterpenoids and total flavonoids is almost higher than that of wild phellinus igniarius, the medicinal requirements are met, the phellinus igniarius cultivation method has a wide development prospect, the investment cost is low, the phellinus igniarius cultivation method is easy to manage, the phellinus igniarius can be used for many years after being put into use once, most mountainous areas in China can be popularized, the resources of local jujube trees and fruit juice factories can be effectively utilized, and the cost is reduced.
The above embodiments are only used for illustrating the technical solution of the present invention, and not for limiting the same; although the invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes may be made and equivalents may be substituted for elements thereof; such modifications and substitutions do not depart from the spirit and scope of the present invention as set forth in the appended claims.
Claims (10)
1. A phellinus igniarius planting method is characterized by comprising the following steps: comprises the following steps of (a) carrying out,
(1) preparing a phellinus igniarius mother culture medium:
the raw material ingredients comprise, by weight, 180 parts of sweet potatoes, 15-20 parts of jujube wood powder, 8-12 parts of corn flour, 7-11 parts of wheat flour, 25-28 parts of agar powder, 6-10 parts of glucose, 15-30 parts of silkworm excrement, 1-1.5 parts of radix angelicae pubescentis powder and 1000 parts of water; cutting sweet potato, decocting in water for 20 min, filtering, adding Zizyphi fructus wood powder, semen Maydis powder, semen Tritici Aestivi powder, agar powder, glucose, faeces Bombycis and radix Angelicae Pubescentis powder, stirring, and autoclaving;
(2) culturing the mother strain of phellinus igniarius:
inoculating the culture medium obtained in the step (1), and culturing the inoculated culture medium in an environment at 28-30 ℃ until the mother strain of phellinus igniarius grows over a culture dish for later use;
(3) preparing a phellinus igniarius fungus bag:
the raw materials comprise, by weight, 100 parts of red date dregs, 20 parts of mulberry leaves, 10 parts of jujube wood powder, 15 parts of corncobs, 2 parts of gypsum, 2 parts of radix angelicae pubescentis powder, 5 parts of bean cakes, 10 parts of wheat bran and 20 parts of silkworm excrement; adding water into the above materials to make the humidity reach 55-60%, and adjusting pH to 7-7.6; pile fermentation for 3 days, and controlling the temperature not to exceed 60 ℃; placing the fermented culture medium into a fungus bag, and sterilizing at 125 deg.C and 0.6Mpa for 3 hr; after sterilization, when the temperature is reduced to 30 ℃, inoculating the phellinus igniarius mother seeds obtained in the step (2) into a phellinus igniarius fungus bag by using an aseptic inoculator; inoculating the phellinus igniarius fungus bags, wherein only one third of the area of one side of each phellinus igniarius fungus bag is inoculated, and marking is carried out through a marking pen;
(4) culturing phellinus igniarius:
placing the phellinus igniarius fungus bag obtained in the step (3) in a greenhouse with 65-75% of shading, placing the inoculation part upwards, maintaining the temperature at 20-28 ℃ and the humidity at 75-85% for culturing, cutting a small opening with the length of 1-3cm in the phellinus igniarius fungus bag inoculation part by using a knife after 25-30 days, and continuing culturing until the phellinus igniarius grows out of the fungus bag;
(5) and (3) rejuvenating and culturing phellinus igniarius:
digging a cultivation groove with the width of 70-120cm and the depth of 35-65cm in the greenhouse, and then spreading lime in the cultivation groove; next, laying an isolation film; then, a water pipe and a ventilation pipe are laid on the isolation film, the water pipe is provided with a water spraying hole, and the ventilation pipe is provided with a ventilation hole; laying aseptic isolation sponge on the water pipe and the ventilation pipe; laying a 10 cm-thick high-temperature steamed date wood section and a 10 cm-thick cortex acanthopanacis section on the upper part of the aseptic isolation sponge, and then spraying a mixture of sterilized cottonseed hulls and cow dung, wherein the length of the date wood section and the length of the cortex acanthopanacis section are 2-10 cm; uniformly mixing 50 parts of red date residues, 15 parts of mulberry leaves, 30 parts of jujube wood powder, 15 parts of corncobs, 2 parts of radix angelicae pubescentis powder, 5 parts of bean cakes, 10 parts of wheat bran and 20 parts of silkworm excrement, paving the mixture on the jujube wood section and the cortex acanthopanacis section after sterilization, transplanting the fungus bags with the phellinus igniarius grown out obtained in the step (4) into a cultivation groove, tearing off half of the fungus bags at the bottom in the cultivation process, then enabling the surface of the mother strain with the phellinus igniarius inoculated with the mark to be upward, and burying the phellinus igniarius fungus bags in a culture medium for two-thirds cultivation; the upper part of the cultivation groove is covered with a heat preservation film; the distance between the cultivation grooves is 30-80 cm; controlling the temperature in the greenhouse to be 25 ℃ and the humidity to be 60%, opening the heat-insulating film for 1-2h every three days, and ventilating the greenhouse for 2 h; spraying water through a water pipe 2 times a day for 3min each time; introducing clean air 2-5 times per day through ventilation pipe, 3-10min each time, introducing air at a temperature 2-3 deg.C higher than room temperature;
(6) picking phellinus igniarius:
after the phellinus igniarius pileus turns yellow brown from light yellow, the pileus is tanned and picked; selecting Phellinus linteus with different cultivation time according to its application in picking process.
2. The phellinus linteus planting method according to claim 1, wherein: the preparation of the phellinus igniarius mother culture medium in the step (1): the raw materials comprise, by weight, 200 parts of sweet potatoes, 20 parts of jujube wood powder, 10 parts of corn flour, 11 parts of wheat flour, 25 parts of agar powder, 8 parts of glucose, 25 parts of silkworm excrement, 1.5 parts of radix angelicae pubescentis powder and 1000 parts of water.
3. The phellinus linteus planting method according to claim 1, wherein: the upper edge of the cultivation groove is 5-20cm higher than the phellinus igniarius fungus bag, so that film covering is facilitated, and influence on growth of phellinus igniarius in the film covering process is reduced.
4. The phellinus linteus planting method according to claim 1, wherein: one end of the bottom of the cultivation groove is higher and the other end is lower, and the cultivation groove is inclined at an angle of 13-20 degrees; set up the return water chamber in the one end of lower side for water in the water pipe is spraying the back, and unnecessary water can flow back down the return water chamber along the barrier film in the cultivation groove.
5. The Phellinus linteus planting method according to claim 4, wherein: the air in the ventilation pipe is introduced from the lower side of the bottom of the cultivation tank.
6. The phellinus linteus planting method according to claim 1, wherein: after 24 months, the liquid nutrient solution is sprayed every 2 months.
7. The phellinus linteus planting method according to claim 1, wherein: an isolation groove is also arranged at the position of 10cm away from the edge of the cultivation groove, the width of the isolation groove is 3-5cm, the depth of the isolation groove is 5-10cm, and white lime is scattered in the isolation groove; the white lime is 3-5cm away from the upper edge of the isolation groove.
8. The phellinus linteus planting method according to claim 7, wherein: the isolation groove can contain the clamping of the film clamping rod, the film clamping rod is trapezoidal, and the film clamping rod is pressed on the film and is reversely buckled into the isolation groove to cover the film on the upper portion of the cultivation groove.
9. The phellinus linteus planting method according to claim 1, wherein: in the step (5), the mixing proportion of the date wood segment, the cortex acanthopanacis segment, the cottonseed hulls and the cow dung is 10 parts by weight: 5: 6: 3.
10. the phellinus linteus planting method according to claim 8, wherein: and (5) adding 3 parts of jujube leaves into the mixture of the jujube wood segments, the cortex acanthopanacis segments, the cottonseed hulls and the cow dung.
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| CN113016503B (en) * | 2021-03-15 | 2023-04-18 | 广西民族师范学院 | Phellinus igniarius wild-imitating cultivation method |
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