CN111773161A - Prebiotic composition and preparation method and application thereof - Google Patents

Prebiotic composition and preparation method and application thereof Download PDF

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CN111773161A
CN111773161A CN202010547832.0A CN202010547832A CN111773161A CN 111773161 A CN111773161 A CN 111773161A CN 202010547832 A CN202010547832 A CN 202010547832A CN 111773161 A CN111773161 A CN 111773161A
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prebiotic composition
skin
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CN111773161B (en
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李占鸿
夏亚平
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Guangzhou Unes Biotechnology Co ltd
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    • A61K2800/00Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
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Abstract

The invention provides a prebiotic composition and a preparation method and application thereof, wherein the prebiotic composition comprises the following raw materials in parts by mass: 10-50 parts of chlorella extract, 0.5-5 parts of tetrahydro-methyl pyrimidine carboxylic acid, 10-40 parts of inulin, 5-30 parts of oat coarse powder extract, 10-40 parts of fructo-oligosaccharide, 3-20 parts of wild soybean fiber and 3-20 parts of glycosaminoglycans. According to the invention, the multiple components are combined to prepare the prebiotic composition, so that the vitality of probiotics can be increased while the skin is helped to generate more probiotics, harmful bacteria can be better inhibited, a healthy and harmonious microbial group is constructed on the surface of the skin, the defense capability and the metabolic capability of the skin are increased, and various skin problems caused by the harmful bacteria, such as improvement of skin problems of acne, hormone-dependent dermatitis and the like, are avoided and relieved.

Description

Prebiotic composition and preparation method and application thereof
Technical Field
The invention belongs to the technical field of beauty and skin care products, and particularly relates to a prebiotic composition and a preparation method and application thereof.
Background
Prebiotics are organic substances that promote the metabolism and proliferation of beneficial bacteria in the body, thereby improving health. The prebiotics can not be decomposed, absorbed and utilized by human body, and after reaching the colon through the digestive tract, some prebiotics can be decomposed and utilized by colon flora to promote the growth of the colon flora, thus having important significance in improving intestinal microecology and promoting lipid, protein and mineral metabolism.
With the improvement of living standard, people pay more and more attention to skin health, and the demand of skin care products is gradually increased. The micro-ecological barrier is an important barrier for maintaining the health of the skin, and when the micro-ecology of the skin is unbalanced, various skin problems are caused. In view of the improvement of the microbial balance of prebiotics, some manufacturers attempt to add prebiotics to skin care products to regulate the microbial balance of the skin surface, ensure better absorption of nutrients by the skin, maintain normal physiological functions, resist external irritation and invasion, help reduce melanin formation, and help maintain the skin in a healthy state. However, the existing prebiotics skin care product generally uses single prebiotics, so that the skin care product has a single effect and cannot meet the requirements of consumers.
Disclosure of Invention
In view of the problems in the prior art, a first object of the present invention is to provide a prebiotic composition capable of comprehensively improving various skin problems caused by harmful bacteria.
The inventor finds that when the human microbiome is disordered, skin problems such as allergy, inflammation, acne, barrier damage and the like are caused, beneficial bacteria in the skin are damaged if means such as sterilization and anti-inflammation are indiscriminately used, drug resistance is generated, and super bacteria are cultured. The introduction of prebiotics has long been used medically to interfere with the ecological balance of the colonies in the body, and this is also effective on the microbiome of the skin. In order to construct a more harmonious microbial group, the inventor respectively carries out comprehensive consideration on the aspects of nutrition supply, competitive mechanism, renewal promotion, immunity activation and the like, and selects and combines several components such as chlorella extract, tetrahydro-methyl pyrimidine carboxylic acid, inulin, oat coarse powder extract, fructo-oligosaccharide, wild soybean fiber, glucosamino glucose and the like, the action mechanisms of the components are different, but the components can cooperate with each other to protect probiotics, inhibit harmful bacteria, synergize and jointly create a healthy skin micro-ecology, so that various skin problems caused by the harmful bacteria are comprehensively improved.
The invention provides a prebiotic composition which comprises the following raw materials in parts by mass:
10-50 parts of chlorella extract
0.5-5 parts of tetrahydro-methyl-pyrimidine carboxylic acid
10-40 parts of inulin
5-30 parts of oat coarse powder extract
10-40 parts of fructo-oligosaccharide
3-20 parts of wild soybean fiber
3-20 parts of glucosaminoglucan
Preferably, the prebiotic composition comprises the following raw materials in parts by mass:
15-45 parts of chlorella extract
0.5-5 parts of tetrahydro-methyl-pyrimidine carboxylic acid
10-30 parts of inulin
5-15 parts of oat coarse powder extract
10-30 parts of fructo-oligosaccharide
Wild soybean fiber 5-15 parts
5-10 parts of glucosaminoglucan
The second purpose of the invention is to provide a preparation method of the prebiotic composition, which comprises the following steps:
(1) mixing Chlorella extract and herba Avenae Fatuae coarse powder extract, and drying;
(2) dissolving inulin, fructo-oligosaccharide and wild soybean fiber in water, freezing, and drying;
(3) and (3) uniformly mixing the dried powder obtained in the steps (1) and (2) with the tetrahydro-methyl pyrimidine carboxylic acid and the glucosamino glucan.
The preparation method of the chlorella extract comprises the following steps:
1) soaking chlorella to obtain a soaking solution;
2) carrying out suction filtration on the soak solution, taking supernatant of the filtrate, and adding protease for enzymolysis;
3) carrying out suction filtration on the liquid obtained in the step 2), and adding pectinase and cellulase into the supernatant of the filtrate for enzymolysis;
4) centrifuging to obtain Chlorella extract.
In the step 1), the chlorella is powdery or muddy; the soaking temperature is 60-70 ℃; the soaking time is 2-4 h.
In the step 2), the enzymolysis temperature is 50-60 ℃; the enzymolysis time is 1-2 h.
In the step 3), the enzymolysis temperature is 40-50 ℃; the enzymolysis time is 1-2 h.
The oat meal extract is prepared by a common enzymatic hydrolysis method.
A third object of the present invention is to provide the use of the prebiotic composition described above in skin care products.
The skin care product comprises prebiotics facial cleanser, prebiotics balance water, prebiotics balance milk and prebiotics balance cream.
The prebiotics facial cleanser comprises the following raw materials in parts by mass:
1-3 parts of prebiotic composition
15-20 parts of cocoyl sodium glycinate
15-20 parts of glycerol
3-6 parts of sodium lauroyl glutamate
5-10 parts of cocamidopropyl betaine
0.5-1 part of sodium isostearoyl lactylate
0.3-3 parts of auxiliary components.
The auxiliary components comprise at least one of thickening agent, pearling agent, preservative and essence.
The preparation method of the prebiotics facial cleanser comprises the following steps:
1) mixing water, glycerol, sodium cocoyl glycinate, sodium lauroyl glutamate, cocamidopropyl betaine and sodium isostearoyl lactylate, and stirring for dissolving;
2) adding prebiotic composition and adjuvant, and stirring.
In the step 1), the stirring and dissolving temperature is 80-100 ℃, and the preferred temperature is 80 ℃; and preserving heat for 20-30min after complete dissolution.
In step 2), cooling to 45 ℃ or below, and then adding the prebiotic composition and the auxiliary ingredients.
The prebiotics balance water comprises the following raw materials in parts by mass:
1-5 parts of prebiotic composition
3-6 parts of glycerol
3-6 parts of butanediol
Saccharide isomerate 0.3-0.8 parts
0.5-2 parts of dendrobium officinale extract
0.5-3 parts of beta-glucan
0.2-2 parts of auxiliary components.
The auxiliary components comprise preservative, essence, solubilizer and the like.
The preparation method of the prebiotics equilibrium water comprises the following steps:
1) mixing water, glycerol, butanediol, and saccharide isomerate, stirring and dissolving;
2) adding prebiotics composition, herba Dendrobii extract, beta-dextran and adjuvant, and stirring.
In the step 1), the stirring and dissolving temperature is 80-100 ℃, and the preferred temperature is 80 ℃; and preserving heat for 20-30min after complete dissolution.
In the step 2), cooling to 45 ℃ or below, and then adding the prebiotic composition, the dendrobium officinale extract, the beta-glucan and the auxiliary components.
The prebiotics balance milk comprises the following raw materials in parts by mass:
2-5 parts of prebiotic composition
3-6 parts of glycerol
Isononyl isononanoate 3-8 parts
2-5 parts of caprylic/capric triglyceride
1-2 parts of polydimethylsiloxane
0.5 to 2 portions of squalane
0.5 to 1.5 portions of methyl glucose sesquistearate
PEG-20 methyl glucose sesquistearate 0.5-1.5 parts
0.5-2 parts of composition of cetearyl olive oleate and sorbitan olive oleate
0.2-2 parts of cetearyl alcohol
0.2-2 parts of PEG/PPG-17/6 copolymer
0.5-3 parts of auxiliary components.
The auxiliary components comprise a thickening agent, a preservative, essence and the like.
The preparation method of the prebiotics balance milk comprises the following steps:
1) mixing water, glycerol, PEG/PPG-17/6 copolymer to obtain water phase;
2) mixing isononyl isononanoate, caprylic/capric triglyceride, polydimethylsiloxane, squalane, methyl glucose sesquistearate, PEG-20 methyl glucose sesquistearate, a composition of cetearyl olive oleate and sorbitan olive oleate, and cetearyl alcohol to obtain an oil phase;
3) mixing the water phase and the oil phase, and emulsifying and homogenizing;
4) adding prebiotic composition and adjuvant, and stirring.
The mixing temperature in step 1) and step 2) is 80-100 ℃, preferably 80 ℃.
The emulsifying and homogenizing temperature in the step 3) is 75-80 ℃, and the emulsifying and homogenizing time is 2-5 min; emulsifying, homogenizing, and keeping the temperature for 20-30 min.
In step 4), cooling to 45 ℃ or below, and then adding the prebiotic composition and the auxiliary ingredients.
The prebiotics balance cream comprises the following raw materials in parts by mass:
3-8 parts of prebiotic composition
3-6 parts of glycerol
Isononyl isononanoate 5-10 parts
3-8 parts of caprylic/capric triglyceride
1-2 parts of polydimethylsiloxane
0.5-2 parts of jojoba oil
0.5-2 parts of shea butter
1-2 parts of octyl polymethylsiloxane
0.5-2 parts of methyl glucose sesquistearate
0.5-2 parts of PEG-20 methyl glucose sesquistearate
1-4 parts of composition of cetearyl alcohol olive oleate and sorbitan olive oleate
0.5-3 parts of cetostearyl alcohol
0.3-1.5 parts of acryloyl dimethyl ammonium taurate/VP copolymer
0.2 to 2 portions of trehalose
0.01-0.1 part of sodium hyaluronate
0.5-5 parts of auxiliary components.
The auxiliary components comprise a thickening agent, a preservative, essence and the like.
The preparation method of the prebiotics balance milk comprises the following steps:
1) mixing water, glycerol, trehalose and sodium hyaluronate to obtain a water phase;
2) mixing isononyl isononanoate, caprylic/capric triglyceride, polydimethylsiloxane, jojoba oil, shea butter, octyl methicone, methylglucose sesquistearate, PEG-20 methylglucose sesquistearate, a composition of cetearyl olive oleate and sorbitan olive oleate, and cetearyl alcohol to obtain an oil phase;
3) mixing the water phase and the oil phase, and emulsifying and homogenizing;
4) adding acryloyl dimethyl ammonium taurate/VP copolymer, homogenizing;
5) adding prebiotic composition and adjuvant, and stirring.
The mixing temperature in step 1) and step 2) is 80-100 ℃, preferably 80 ℃.
The emulsifying and homogenizing temperature in the step 3) is 75-80 ℃, and the emulsifying and homogenizing time is 2-5 min; emulsifying, homogenizing, and keeping the temperature for 20-30 min.
Cooling to 70 ℃ or below in the step 4), and then adding the acryloyl dimethyl ammonium taurate/VP copolymer; and 4) homogenizing for 2-5 min.
In step 5), cooling to 45 ℃ or below, and then adding the prebiotic composition and the auxiliary ingredients.
Compared with the prior art, the invention has the following beneficial effects:
the invention prepares the prebiotic composition by combining a plurality of components, provides necessary food for probiotics by utilizing inulin, oat coarse powder extract, fructo-oligosaccharide and wild soybean fiber, and promotes the growth and proliferation of skin probiotics; meanwhile, chlorella extract, tetrahydro-methyl pyrimidine carboxylic acid and glucoseamine are used for promoting cell renewal and enhancing immunity. Therefore, the prebiotic composition can help the skin to generate more probiotics and simultaneously increase the activity of the probiotics, so that harmful bacteria can be better inhibited, a healthy and harmonious microbial group is constructed on the surface of the skin, the defense capability and the metabolic capability of the skin are increased, and various skin problems caused by the harmful bacteria, such as improvement of skin problems of acne, hormone-dependent dermatitis and the like, are avoided and relieved.
Detailed Description
The present invention will be described in further detail with reference to specific examples. The starting materials in the following examples are all commercially available in a conventional manner unless otherwise specified.
The invention provides a prebiotic composition, which comprises the following raw materials in proportion as shown in table 1:
TABLE 1 raw materials and amounts of prebiotic compositions
Figure BDA0002541379390000061
Figure BDA0002541379390000071
The preparation method of the prebiotic composition comprises the following steps:
(1) mixing Chlorella extract and herba Avenae Fatuae coarse powder extract, and drying;
(2) dissolving inulin, fructo-oligosaccharide and wild soybean fiber in water, freezing, and drying;
(3) and (3) uniformly mixing the dried powder obtained in the steps (1) and (2) with the tetrahydro-methyl pyrimidine carboxylic acid and the glucosamino glucan.
The preparation method of the chlorella extract comprises the following steps:
1) making Chlorella into powder or paste, soaking in 70 deg.C warm water, and keeping the temperature for 4 hr to obtain soaking solution.
2) And (3) carrying out suction filtration on the soak solution, taking supernatant of the filtrate, adding protease, and keeping the temperature at 60 ℃ for 1 h.
3) And (4) performing suction filtration again to obtain supernatant, continuously adding pectinase and cellulase, and keeping the temperature at 50 ℃ for 1 h.
4) Centrifuging to obtain Chlorella extract.
The oat meal extract is prepared by a common enzymatic hydrolysis method.
The invention also utilizes the prebiotics to respectively prepare a prebiotics cleansing milk, a prebiotics balancing water, a prebiotics balancing milk and a prebiotics balancing cream, and the raw materials and the mixture ratio of various skin care products are as follows:
the prebiotics facial cleanser comprises the following raw materials:
prebiotic composition 2 parts
18 parts of cocoyl sodium glycinate
16 portions of glycerol
4 parts of sodium lauroyl glutamate
6 parts of cocamidopropyl betaine
Sodium isostearoyl lactylate 0.8 parts
Auxiliary ingredient 1.6 parts
And water is added to make up to 100 parts.
Wherein the auxiliary components comprise thickening agent, preservative and essence.
The prebiotics facial cleanser can be prepared according to the following method:
1) adding water, glycerol, sodium cocoyl glycinate, sodium lauroyl glutamate, cocamidopropyl betaine, and sodium isostearoyl lactylate into stirring pot, stirring and heating to 80 deg.C, dissolving completely, and keeping the temperature for 20min
2) Cooling to 45 deg.C, adding prebiotic composition and adjuvant, and stirring.
A prebiotics balance water comprises the following raw materials:
prebiotic composition 2 parts
3 portions of glycerin
Butanediol 5 parts
Saccharide isomerate 0.5 part
2 parts of dendrobium officinale extract
2 portions of beta-glucan
0.8 part of auxiliary component
And water is added to make up to 100 parts.
Wherein the auxiliary components comprise thickening agent, preservative and essence.
The prebiotics balance water can be prepared according to the following method:
1) putting water, glycerol, butanediol, and saccharide isomerate into a stirring pot, stirring and heating to 80 deg.C, dissolving completely, and keeping the temperature for 20 min.
2) Cooling to 45 deg.C, adding prebiotic composition, herba Dendrobii extract, beta-dextran and adjuvant, and stirring.
A prebiotics balance milk comprises the following raw materials:
prebiotic composition 3 parts
5 parts of glycerin
Isononyl isononanoate 6 parts
Caprylic/capric triglyceride 3 parts
Polydimethylsiloxane 2 parts
1 part of squalane
1 part of methyl glucose sesquistearate
PEG-20 methyl glucose sesquistearate 1 part
Composition of cetearyl olive oleate and sorbitan olive oleate 1.5 parts
Cetostearyl alcohol 1 part
1 part of PEG/PPG-17/6 copolymer
Auxiliary ingredient 2 parts
And water is added to make up to 100 parts.
Wherein the auxiliary components comprise thickening agent, preservative and essence.
The prebiotics balance milk can be prepared according to the following method:
1) putting water, glycerol and PEG/PPG-17/6 copolymer into a water phase pot, and heating to 80 deg.C to obtain water phase for use.
2) Adding isononyl isononanoate, caprylic/capric triglyceride, polydimethylsiloxane, squalane, methyl glucose sesquistearate, PEG-20 methyl glucose sesquistearate, a composition of cetearyl olive oleate and sorbitan olive oleate, and cetearyl alcohol into an oil phase pot, heating to 80 deg.C, and melting to obtain an oil phase for later use.
3) Starting the emulsifying pot, stirring, sequentially pumping the water phase and the oil phase into the emulsifying pot, homogenizing for 5min, and keeping the temperature at 80 deg.C for 20 min.
4) Cooling to 45 deg.C, adding prebiotic composition and adjuvant, and stirring.
A prebiotics balance cream comprises the following raw materials:
prebiotic composition 6 parts
5 parts of glycerin
Isononyl isononanoate 8 parts
Caprylic/capric triglyceride 6 parts
Polydimethylsiloxane 2 parts
Jojoba oil 1 part
Shea butter 1 part
Octyl polymethylsiloxane 2 parts
1.5 parts of methyl glucose sesquistearate
PEG-20 methyl glucose sesquistearate 1.5 parts
Composition of cetearyl olive oleate and sorbitan olive oleate 3.5 parts
Cetostearyl alcohol 2.5 parts
1 part of acryloyl dimethyl ammonium taurate/VP copolymer
Trehalose 0.8 part
0.05 part of sodium hyaluronate
Auxiliary ingredient 2.6 parts
And water is added to make up to 100 parts.
Wherein the auxiliary components comprise thickening agent, preservative and essence.
The prebiotics balance cream can be prepared according to the following method:
1) putting water, glycerol, trehalose and sodium hyaluronate into a water phase pot, heating to 80 ℃, and dissolving to obtain a water phase for later use.
2) Adding isononyl isononanoate, caprylic/capric triglyceride, polydimethylsiloxane, jojoba oil, shea butter, octyl methicone, methyl glucose sesquistearate, PEG-20 methyl glucose sesquistearate, composition of cetearyl olive oleate and sorbitan olive oleate, and cetearyl alcohol into an oil phase pot, heating to 80 deg.C, and melting to obtain oil phase.
3) Starting the emulsifying pot, stirring, sequentially pumping the water phase and the oil phase into the emulsifying pot, homogenizing for 5min, and keeping the temperature at 80 deg.C for 20 min.
4) Cooling to 70 deg.C, adding acryloyl dimethyl ammonium taurate/VP copolymer, homogenizing for 5min
5) Continuously cooling to 45 deg.C, adding prebiotic composition and adjuvant, and stirring.
Wherein the prebiotic composition used for the prebiotic facial cleanser, the prebiotic balance water, the prebiotic balance milk and the prebiotic balance cream is selected from examples 1-5 and comparative examples 1-7.
Performance testing
1. Treatment and inhibition of acne
(1) Testing materials: a cleanser and balance water prepared using the prebiotic compositions of examples 1-5 and comparative examples 1-7.
(2) Test objects: 20 volunteers with acne aged between 17-32 years, 14 men and 6 women.
(3) The test method comprises the following steps: the above volunteers were divided into 4 groups, and skin improvement was tested after 4 weeks using a facial cleanser containing the compositions of example 1, example 3, comparative example 2 and comparative example 3 and a balance of water (after washing the face with the facial cleanser each day, the face was rubbed with the balance of water).
(4) The test results are shown in the following table:
Figure BDA0002541379390000111
according to the test results, the prebiotic composition can be added into the skin care product to effectively reduce skin redness, blister infection and inflammation and improve the skin hydration degree. When the prebiotic composition omits the tetrahydro-methyl pyrimidine carboxylic acid or the inulin, the skin care product has nearly half effect on reducing skin redness and pimple infection, and has obviously reduced effects on improving skin inflammation and increasing skin hydration degree.
2. Ameliorating effect on hormone-dependent dermatitis
(1) Testing materials: a cleanser, balance water and balance cream prepared using the prebiotic compositions of examples 1-5 and comparative examples 1-7.
(2) Test objects: 16 volunteers with hormone-dependent dermatitis aged between 38-60 years, female.
(3) The test method comprises the following steps: the above volunteers were divided into 2 groups, and used face cleansers, balance water and balance cream containing the compositions of example 4 and comparative example 4, respectively (after washing the face with the face cleansers every day, the face was rubbed with the balance water and balance cream), and skin conditions were tested after 8 weeks, and questionnaires were filled.
(4) The test results are shown in the following table:
Figure BDA0002541379390000112
Figure BDA0002541379390000121
according to the test results in the table above, it can be found that the addition of the prebiotic composition to the skin care product can effectively improve the hormone-dependent dermatitis; and the improvement effect of the skin care product on the hormone-dependent dermatitis is obviously reduced after the oat extract is omitted from the prebiotic composition.
3. Improving effect on skin
(1) Testing materials: face cleansers, balance water, balance milk and balance cream prepared using the prebiotic compositions of examples 1-5 and comparative examples 1-7.
(2) Test objects: 36 volunteers aged 18-60 years, 10 males and 26 females.
(3) The test method comprises the following steps: the above volunteers were divided into 6 groups, and used face toilet, balance water, balance milk and balance cream containing compositions of example 2, example 5, comparative example 1, comparative example 5, comparative example 6 and comparative example 7, respectively (the face was rubbed with the balance water, balance milk and balance cream after washing the face with the face toilet daily), and skin conditions were tested after 6 weeks, and questionnaires were filled.
(4) The test results are shown in the following table:
Figure BDA0002541379390000122
when the chlorella extract is omitted from the prebiotic composition (comparative example 1), although the skin care product can effectively improve the moisture retention of the skin, reduce pores and improve the skin luster, the skin elasticity is difficult to improve; after fructo-oligosaccharide (comparative example 5) or glucosaminoglycan (comparative example 7) is omitted, the skin care product cannot effectively improve the moisture retention, the fineness, the glossiness and the elasticity of the skin; when the soybean fibers were omitted (comparative example 6), the skin care product was excellent in moisturizing effect, but poor in the smoothness, glossiness and elasticity.
In conclusion, the chlorella extract, the tetrahydro-methyl pyrimidine carboxylic acid, the inulin, the oat coarse powder extract, the fructo-oligosaccharide, the wild soybean fiber and the glucosaminodextran are jointly prepared into the prebiotic composition and are used for preparing the skin care product, so that the skin quality can be comprehensively improved, particularly, the skin with the problems of acne, hormone-dependent dermatitis and the like with the damaged microbial ecosystem can be excellently improved, and the comprehensive improvement effect cannot be realized after certain components are omitted from the prebiotic composition.
The above embodiments are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be regarded as equivalent replacements within the protection scope of the present invention.

Claims (10)

1. A prebiotic composition, comprising: the composite material comprises the following raw materials in parts by mass:
10-50 parts of chlorella extract
0.5-5 parts of tetrahydro-methyl-pyrimidine carboxylic acid
10-40 parts of inulin
5-30 parts of oat coarse powder extract
10-40 parts of fructo-oligosaccharide
3-20 parts of wild soybean fiber
3-20 parts of glucosaminoglucan.
2. A process for the preparation of a prebiotic composition according to claim 1 which comprises: the method comprises the following steps:
(1) mixing Chlorella extract and herba Avenae Fatuae coarse powder extract, and drying;
(2) dissolving inulin, fructo-oligosaccharide and wild soybean fiber in water, freezing, and drying;
(3) and (3) uniformly mixing the dried powder obtained in the steps (1) and (2) with the tetrahydro-methyl pyrimidine carboxylic acid and the glucosamino glucan.
3. The method of claim 2, wherein: the preparation method of the chlorella extract comprises the following steps:
1) soaking chlorella to obtain a soaking solution;
2) carrying out suction filtration on the soak solution, taking supernatant of the filtrate, and adding protease for enzymolysis;
3) carrying out suction filtration on the liquid obtained in the step 2), and adding pectinase and cellulase into the supernatant of the filtrate for enzymolysis;
4) centrifuging to obtain Chlorella extract.
4. The method according to claim 3, wherein: in the step 2), the enzymolysis temperature is 50-60 ℃.
5. The method according to claim 3, wherein: in the step 3), the enzymolysis temperature is 40-50 ℃.
6. Use of the prebiotic composition of claim 1 in a skin care product.
7. A prebiotics facial cleanser is characterized in that: the composite material comprises the following raw materials in parts by mass:
1-3 parts of prebiotic composition according to claim 1
15-20 parts of cocoyl sodium glycinate
15-20 parts of glycerol
3-6 parts of sodium lauroyl glutamate
5-10 parts of cocamidopropyl betaine
0.5-1 part of sodium isostearoyl lactylate
0.3-3 parts of auxiliary components.
8. A prebiotics balance water is characterized in that: the composite material comprises the following raw materials in parts by mass:
1-5 parts of prebiotic composition according to claim 1
3-6 parts of glycerol
3-6 parts of butanediol
Saccharide isomerate 0.3-0.8 parts
0.5-2 parts of dendrobium officinale extract
0.5-3 parts of beta-glucan
0.2-2 parts of auxiliary components.
9. A prebiotics balance milk, which is characterized in that: the composite material comprises the following raw materials in parts by mass:
2-5 parts of prebiotic composition according to claim 1
3-6 parts of glycerol
Isononyl isononanoate 3-8 parts
2-5 parts of caprylic/capric triglyceride
1-2 parts of polydimethylsiloxane
0.5 to 2 portions of squalane
0.5 to 1.5 portions of methyl glucose sesquistearate
PEG-20 methyl glucose sesquistearate 0.5-1.5 parts
0.5-2 parts of composition of cetearyl olive oleate and sorbitan olive oleate
0.2-2 parts of cetearyl alcohol
0.2-2 parts of PEG/PPG-17/6 copolymer
0.5-3 parts of auxiliary components.
10. A prebiotic balance cream is characterized in that: the composite material comprises the following raw materials in parts by mass:
3-8 parts of prebiotic composition according to claim 1
3-6 parts of glycerol
Isononyl isononanoate 5-10 parts
3-8 parts of caprylic/capric triglyceride
1-2 parts of polydimethylsiloxane
0.5-2 parts of jojoba oil
0.5-2 parts of shea butter
1-2 parts of octyl polymethylsiloxane
0.5-2 parts of methyl glucose sesquistearate
0.5-2 parts of PEG-20 methyl glucose sesquistearate
1-4 parts of composition of cetearyl alcohol olive oleate and sorbitan olive oleate
0.5-3 parts of cetostearyl alcohol
0.3-1.5 parts of acryloyl dimethyl ammonium taurate/VP copolymer
0.2 to 2 portions of trehalose
0.01-0.1 part of sodium hyaluronate
0.5-5 parts of auxiliary components.
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