CN110755345A - Prebiotic composition and application thereof - Google Patents
Prebiotic composition and application thereof Download PDFInfo
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- CN110755345A CN110755345A CN201911046600.0A CN201911046600A CN110755345A CN 110755345 A CN110755345 A CN 110755345A CN 201911046600 A CN201911046600 A CN 201911046600A CN 110755345 A CN110755345 A CN 110755345A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
- A61K8/606—Nucleosides; Nucleotides; Nucleic acids
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/005—Preparations for sensitive skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
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Abstract
The invention discloses a prebiotic composition and application thereof, wherein the prebiotic composition comprises, by mass, 5-15 parts of dendrobium officinale extract, 0.5-2 parts of ribose and 0.5-2 parts of β glucan, and the prebiotic composition comprises the dendrobium officinale extract, the ribose and β glucan, and the dendrobium officinale has the effects of scavenging free radicals, improving the activity of antioxidase and inhibiting lipid peroxidation and protecting a biological membrane.
Description
Technical Field
The invention relates to the technical field of cosmetics, and particularly relates to a prebiotic composition and application thereof.
Background
Under normal conditions, a large number of microorganisms exist on the surface of human skin, including bacteria, fungi, viruses, chlamydia, certain protozoa and the like, microbial flora forms a microecological system similar to an ecosphere, and different strains are mutually dependent and restricted to form a unique ecological structure, namely the normal skin microbial flora. According to the research of skin microecology, the skin resident bacteria have the effect of occupying space and protecting, and are orderly planted on the skin like a biological barrier, so that the pathogenic bacteria and the foreign nations cannot stand on the skin. However, long-term, repeated and excessive internal and external environmental stimulation acts on the skin, and when the compensation capacity of the skin is exceeded, the ecological balance of the skin is broken, so that various skin diseases are caused. Most of the existing cosmetics on the market are designed artificially, although the effect is good, the cosmetics often contain various components harmful to human cells, such as heavy metals, hormones and the like, the effects of the cosmetics are mostly limited to moisturizing, whitening and the like, and dependence and damage to the body can be caused after long-term use. The skin moisturizing and whitening cream has no good regulating effect on skin micro-ecological balance and skin state, cannot well solve the skin problem caused by skin micro-ecological imbalance, has only surface layer effect, cannot achieve deep and continuous skin care effect, and also does not have the functions of cell repairing and cutin barrier repairing.
Prebiotics refer to a combination of substances that can be utilized by a selected microorganism (e.g., a skin commensal microorganism or a gastrointestinal microorganism) as a nutrient, can induce growth activity and replication of the selected microorganism, can be utilized by the selected microorganism as an energy source, and can be used by the selected microorganism for the production of biomolecules (i.e., RNA, DNA, and proteins). Non-limiting examples of prebiotics include mucopolysaccharides, oligosaccharides such as galactooligosaccharides ("GOS"), polysaccharides, amino acids, vitamins, nutrient precursors, metabolites of the collected organism, lipids, proteins, and the like. There are also studies in the present study relating to the regulation of skin micro-ecology and prebiotic compositions, but prebiotic compositions are often used for the treatment of skin inflammation and the like. CN110179743A discloses a composition for regulating skin micro-ecological balance and a preparation method thereof, wherein the composition regulates flora growth and skin pH through the combined action of prebiotics, metagens and biological polypeptides, but the composition has no direct effect on skin micro-ecological regulation, and only subjective relativity evaluation can not confirm whether the composition can effectively regulate the balance of beneficial flora and harmful flora in the skin ecosystem and can synergistically promote related skin care functions.
Disclosure of Invention
The invention aims to solve the technical problems that the existing skin care products do not have the cell repair capability and the function of repairing cutin barriers and cannot solve the defects and the defects of skin problems caused by the unbalance of skin microecology, and provides a prebiotic composition.
The invention also aims to provide the application of the prebiotic composition in preparing a preparation with the effect of regulating the ecological balance of skin.
The invention also aims to provide application of the prebiotic composition in preparing a preparation with the effects of inhibiting the reproduction of staphylococcus aureus and escherichia coli and promoting the reproduction of staphylococcus epidermidis and bifidobacterium.
Still another object of the present invention is to provide a use of prebiotic composition for preparing a preparation having an effect of increasing hyaluronic acid content in skin.
Still another object of the present invention is to provide a preparation having the effect of regulating the ecological balance of the skin.
The above purpose of the invention is realized by the following technical scheme:
the prebiotic composition comprises, by mass, 5-15 parts of a dendrobium officinale extract, 0.5-2 parts of ribose and 0.1-0.5 part of β glucan.
The main performance parameters of the ribose of the invention are as follows: is derived from corn seed extract, and the content of effective components is more than or equal to 99%.
β the content of active components of dextran is not less than 99%.
The prebiotic composition comprises the dendrobium officinale extract, the main component of the dendrobium officinale extract is dendrobium officinale polysaccharide, the polysaccharide substance in the traditional cosmetics generally has the main function of moisturizing, the anti-allergy, anti-inflammation and damaged cell repairing effects are not obvious, and the prebiotic composition has no promotion effect on the growth of probiotics. The dendrobium candidum extract is preferably extracted from dendrobium candidum in Yunnan, is rich in various active ingredients such as polysaccharide, and the polysaccharide has the activities of removing free radicals, improving the activity of antioxidant enzyme and inhibiting lipid peroxidation, and plays a role in protecting a biological membrane. Moreover, the dendrobium officinale can improve the contents of superoxide dismutase (SOD), Peroxidase (POD) and Catalase (CAT), the superoxide dismutase (SOD) is one of the most effective antioxidant enzymes, superoxide anions and hydrogen peroxide can be removed, and the damage to a system is reduced; peroxidase (POD) mainly has the functions of eliminating active oxygen and hydrogen peroxide damage; catalase (CAT) is an enzyme scavenger, also known as a catalyst. He can promote H2O2Breakdown and thus is one of the key enzymes of the biological defense system. The dendrobium officinale extract has strong oxidation resistance, and the dendrobium officinale polysaccharide has good oxidation resistance activity under alkaline conditions. Long-term experiments of the inventor show that the dendrobium officinale can obviously improve the levels of superoxide dismutase (SOD) and serum prolyl-N-Hydroxyzine (HYP), and obviously reduce lipid peroxide (LOP) and monoamine oxide (MAO), thereby playing the role of protecting a biological membrane.
The dendrobium candidum polysaccharide is soluble in water, is compounded with β glucan and ribose, has the effects of resisting allergy, diminishing inflammation and repairing damaged cells when being applied to a composition for regulating the ecological balance of skin, keeps moisture, and can regulate the micro-ecological balance environment of the skin, increase the number of beneficial bacteria in the skin and inhibit the growth of harmful bacteria when being compounded with metabolites of probiotics.
The quality compatibility of the dendrobium officinale extract, the ribose and the β glucan in the prebiotic composition is crucial, wherein the skin feeling of the composition is poor due to excessive quality of any component, and the production cost is increased.
The dendrobium officinale extract, the ribose and the β glucan are subjected to synergistic compounding of specific components, so that nutrients can be provided for probiotics on the surface of the skin, the number of beneficial bacteria on the skin can be increased, the growth of harmful bacteria can be inhibited, and active oxygen free radicals on the surface of the skin can be eliminated, so that the problem of the skin caused by the unbalance of skin micro-ecology can be solved, and the skin can be smooth, bright and white while cells are self-repaired and the skin barrier is enhanced.
Preferably, the prebiotic composition comprises, by mass, 8-12 parts of dendrobium officinale extract, 0.8-1.2 parts of ribose and 0.1-0.3 part of β glucan.
For example, the extract can be 10 parts of dendrobium officinale extract, 1 part of ribose and 0.2 part of β glucan;
further preferably, the prebiotic composition comprises the following components, by mass, 10 parts of dendrobium officinale extract, 1 part of ribose and 0.2 part of β glucan.
Preferably, the mass content of the dendrobium officinale polysaccharide in the dendrobium officinale extract is 20-30%.
The dendrobium officinale polysaccharide is a main active ingredient in the dendrobium officinale extract, and the inventor finds out by accident that the dendrobium officinale polysaccharide has a good biological membrane protection effect within 20-30% of the content of the dendrobium officinale polysaccharide, and well promotes the growth of probiotics.
The dendrobium officinale extract can improve the activities of superoxide dismutase, peroxidase and catalase.
The superoxide dismutase has the following functions: one of the most effective antioxidant enzymes is superoxide dismutase (SOD), which is a biological enzyme widely existing in the natural world, and can remove superoxide anions and hydrogen peroxide to reduce damage to the system. According to the aging free radical theory, aging is a result of a dysfunction in the production and clearance of free radicals. Under normal conditions, the generation and elimination of free radicals are in a balanced state, but the balance is broken along with the aging of the organism and the influence of external factors, and redundant free radicals can damage the organism through various channels. For example, oxygen radicals can cause lipid peroxidation. The peroxidized lipids will crosslink with the protein, resulting in an insoluble protein. This change is most pronounced with collagen in the connective tissue, which causes the collagen to become tough, shorten in length, lose its turgor, and cause wrinkles. In addition, lipid peroxides are degraded by the action of oxidase to produce chemical substances such as malondialdehyde, which rapidly cross-links with phosphatidylethanolamine in vivo to produce yellow pigments, which are then combined with proteins, nucleic acids, lipids, etc. to produce physiologically inactive tan pigments, so-called age spots. Clinical verification and long-term use show that superoxide dismutase (SOD) is a gram of oxygen free radical, and has the effects of resisting wrinkle, removing speckle and pigment, resisting inflammation, preventing sunburn, delaying skin aging and the like.
More preferably, the mass content of the dendrobium officinale polysaccharide in the dendrobium officinale extract is 25%.
In the application of the prebiotic composition described above in particular,
the application of the prebiotic composition in preparing a preparation with the effect of regulating the ecological balance of the skin is within the protection scope of the invention.
The prebiotic composition comprises dendrobium officinale extract, ribose and β glucan, can inhibit the reproduction of potential pathogenic bacteria such as staphylococcus aureus, escherichia coli and the like, promotes the growth of probiotics such as staphylococcus epidermidis, bifidobacterium and the like, obviously prevents and regulates the flora imbalance and regulates the skin ecological balance.
The prebiotic composition can also promote the restoration of cells, when the skin barrier function is damaged, the division and renewal speed of keratinocytes can be reduced, so that the problems of incomplete peeling of the horny layer or thinning of the horny layer and the like can be solved, and the dendrobium officinale extract in the prebiotic composition can accelerate the renewal speed of the keratinocytes and restore the damaged horny layer, so that the effects of restoring the horny barrier and moisturizing can be achieved.
The application of the prebiotic composition in preparing the preparation with the efficacies of inhibiting the reproduction of staphylococcus aureus and escherichia coli and promoting the reproduction of staphylococcus epidermidis and bifidobacterium is also within the protection scope of the invention.
The application of the prebiotic composition in the preparation of the preparation for improving the effect of the hyaluronic acid content in the skin is also within the protection scope of the invention.
Hyaluronic acid serves not only as a water absorbent in the epidermis, but also functions of regulating the diffusion and movement of proteins and water electrolytes, promoting wound healing, etc., and serves as a natural moisturizing factor in the epidermis, so that when the keratinous barrier is damaged, the expression of hyaluronic acid is reduced, and the barrier function is damaged. The prebiotic composition can improve the content of hyaluronic acid in skin, and achieves the effects of moisturizing and barrier strengthening.
The invention also protects a preparation with the effect of regulating the ecological balance of skin, which comprises the prebiotic composition and other auxiliary materials acceptable for the preparation.
In the production of the actual preparation, the preparation with the effect of regulating the ecological balance of the skin comprises cosmetics and external pharmaceutical preparations, and the preparation forms comprise lotions, emulsions, creams and sprays.
The prebiotic composition formulations of the present invention may also include infant washing and skin care products.
Compared with the prior art, the invention has the beneficial effects that:
(1) the invention provides a prebiotic composition, which comprises dendrobium officinale extract, ribose and β glucan, wherein the polysaccharide has the activities of scavenging free radicals, improving the activity of antioxidant enzyme and inhibiting lipid peroxidation, and has the effect of protecting a biological membrane.
(2) The synergistic compounding of the prebiotic composition disclosed by the invention can not only provide nutrients for probiotics on the surface of the skin, but also increase the number of beneficial bacteria on the skin, inhibit the growth of harmful bacteria and clear away active oxygen radicals on the surface of the skin, so that the skin problem caused by the skin micro-ecological imbalance is solved, cells are self-repaired, and the skin barrier is enhanced.
(3) The prebiotic composition can also improve the content of hyaluronic acid in skin, and achieves the effects of moisturizing and barrier strengthening.
Drawings
Fig. 1 shows that the prebiotic composition was subjected to a cell scratch test to verify cell repair ability.
Fig. 2 is a graph of the effect of prebiotic compositions on the face of a subject to verify their modulating effects on skin microorganisms.
Detailed Description
The present invention will be further described with reference to specific embodiments, but the present invention is not limited to the examples in any way. The starting reagents employed in the examples of the present invention are, unless otherwise specified, those that are conventionally purchased.
Example 1
A prebiotic composition comprises, by mass, 15 parts of a dendrobium officinale extract, 2 parts of ribose and 0.5 part of β glucan.
Wherein the content of polysaccharide in the dendrobium officinale extract is 25 percent,
the sources of the dendrobium officinale extract in the embodiment are as follows: extract of stem of Dendrobium officinale Kimura et Migo in Yunnan province
The ribose source is: activecoverts Inc. of USA
β the source of glucan is IFF-Lucas Meyer Cosmetics, USA
Example 2
A prebiotic composition comprises, by mass, 5 parts of a dendrobium officinale extract, 0.5 part of ribose and 0.1 part of β glucan.
Wherein the content of polysaccharide in the dendrobium officinale extract is 25 percent,
the sources of the dendrobium officinale extract in the embodiment are as follows: extract of stem of Dendrobium officinale Kimura et Migo in Yunnan province
The ribose source is: activecoverts Inc. of USA
β the source of glucan is IFF-Lucas Meyer Cosmetics, USA
Example 3
A prebiotic composition comprises, by mass, 12 parts of a dendrobium officinale extract, 1.2 parts of ribose and 0.3 part of β glucan.
Wherein the content of polysaccharide in the dendrobium officinale extract is 25 percent,
the sources of the dendrobium officinale extract in the embodiment are as follows: extract of stem of Dendrobium officinale Kimura et Migo in Yunnan province
The ribose source is: activecoverts Inc. of USA
β the source of glucan is IFF-Lucas Meyer Cosmetics, USA
Example 4
A prebiotic composition comprises, by mass, 8 parts of a dendrobium officinale extract, 0.8 part of ribose and 0.1 part of β glucan.
Wherein the content of polysaccharide in the dendrobium officinale extract is 25 percent,
the sources of the dendrobium officinale extract in the embodiment are as follows: extract of stem of Dendrobium officinale Kimura et Migo in Yunnan province
The ribose source is: activecoverts Inc. of USA
β the source of glucan is IFF-Lucas Meyer Cosmetics, USA
Example 5
A prebiotic composition comprises, by mass, 10 parts of a dendrobium officinale extract, 1 part of ribose and 0.2 part of β glucan.
Wherein the content of polysaccharide in the dendrobium officinale extract is 25 percent,
the sources of the dendrobium officinale extract in the embodiment are as follows: extract of stem of Dendrobium officinale Kimura et Migo in Yunnan province
The ribose source is: activecoverts Inc. of USA
β the source of glucan is IFF-Lucas Meyer Cosmetics, USA
Comparative example 1
A prebiotic composition comprises, by mass, 1 part of ribose and 0.2 part of β glucan.
The ribose source of this example is:
the ribose source is: activecoverts Inc. of USA
β the source of glucan is IFF-Lucas Meyer Cosmetics, USA
Comparative example 2
A prebiotic composition comprises, by mass, 10 parts of a dendrobium officinale extract, 0.01 part of ribose and 0.2 part of β glucan.
Wherein the content of polysaccharide in the dendrobium officinale extract is 25 percent,
the sources of the dendrobium officinale extract in the embodiment are as follows: extract of stem of Dendrobium officinale Kimura et Migo in Yunnan province
The ribose source is: activecoverts Inc. of USA
β the source of glucan is IFF-Lucas Meyer Cosmetics, USA
Comparative example 3
A prebiotic composition comprises, by mass, 10 parts of Dendrobium officinale extract, 1 part of ribose, and 0.01 part of β glucan
Wherein the content of polysaccharide in the Dendrobium officinale extract is 25%.
The sources of the dendrobium officinale extract in the embodiment are as follows: extract of stem of Dendrobium officinale Kimura et Migo in Yunnan province
The ribose source is: activecoverts Inc. of USA
β the source of glucan is IFF-Lucas Meyer Cosmetics, USA
Comparative example 4
A prebiotic composition comprises, by mass, 10 parts of a dendrobium officinale extract, 0.01 part of ribose and 0.01 part of β glucan.
Wherein the content of polysaccharide in the Dendrobium officinale extract is 25%.
The sources of the dendrobium officinale extract in the embodiment are as follows: extract of stem of Dendrobium officinale Kimura et Migo in Yunnan province
The ribose source is: activecoverts Inc. of USA
β the source of glucan is IFF-Lucas Meyer Cosmetics, USA
Result detection
(1) Detection of the modulation of skin microorganisms
The detection method comprises the following steps: the prebiotic compositions of examples 1-5 and comparative examples 1-4 were tested for their modulating effects on the skin harmful flora staphylococcus aureus, escherichia coli, the beneficial flora staphylococcus epidermidis and bifidobacteria by the following specific procedures:
the experimental method comprises the following steps: inoculating Staphylococcus epidermidis, Bifidobacterium adolescentis, Staphylococcus aureus and Escherichia coli with glass culture dish and conventional culture medium at an amount of 1 × 105Examples 1 to 5 and comparative examples 1 to 4 were then coated on the inoculation surface, and the number of colonies was examined after 24 hours.
The results are shown in table 1:
TABLE 1
As can be seen from the experimental results of table 1 above,
as can be seen from the above table, the compositions prepared in embodiments 1 to 5 of the present invention can effectively promote the proliferation of staphylococcus epidermidis and bifidobacteria in normal skin flora, and have an inhibitory effect on staphylococcus aureus and escherichia coli, which are skin pathogenic bacteria, and particularly, embodiment 5 has a positive effect on enhancing skin micro-ecology.
In contrast, in comparative examples 1 to 4, which do not contain or are not used in the recommended range, the composition for regulating the skin microecological balance according to the present invention has no proliferation effect on staphylococcus epidermidis and bifidobacterium adolescentis, has no inhibitory effect on staphylococcus aureus and escherichia coli, and is less likely to cause skin discomfort due to the fact that the skin is not in the microecological balance after being applied to a human body.
The composition prepared in the comparative example 1 without the dendrobium officinale extract has poor proliferation effect on staphylococcus epidermidis and bifidobacterium adolescentis compared with the composition prepared in the examples 1-5, and the dendrobium officinale extract is used as an antioxidant and plays a certain role in promoting the growth of beneficial bacteria
(2) Moisturizing Performance and skin Barrier repair test
The related moisturizing performance and the skin barrier repairing function are detected through a human body water content experiment and a human body percutaneous water loss experiment.
The specific detection method of the human body water content experiment is as follows:
the moisture retention performance is detected by a skin moisture content tester (Cormeter CM825, Germany CK), the MMV value index of the skin moisture content is measured, and the moisture content of the horny layer of the human skin and the MMV value of the skin moisture content are measured by a capacitance method: the MMV value characterizes skin moisture, the greater the MMV value, the higher the stratum corneum moisture content.
The specific detection method of the human body percutaneous water loss experiment is as follows:
the protective skin barrier is measured by a percutaneous water loss tester (Tewameter TM 300; German CK) and a TEWL value index of percutaneous water loss, the water vapor pressure gradient of different points formed by the water loss of an angle layer of the epidermis is measured by two groups of sensors with different temperatures and humidity, the water content evaporated by the epidermis is measured, the TEWL value is used for evaluating the skin barrier function, the important parameter for inspecting the skin repair after the product is used, the smaller the value is, the better the skin barrier is, and the better the repair is.
The specific detection method comprises the following steps: the inside skin of the arm was cleaned using a cleaning product, sitting still in the laboratory for about 20min, during which the test area was marked on the inside of the arm by the laboratory. And after the test preparation process is finished, acquiring initial skin value data for the first time. And after the collection of the skin initial value data is finished, smearing the test sample. A tester uses a pipette to quantitatively sample (0.02g) in a corresponding area and smear a sample in the same direction, data are collected 2h and 4h after the sample is smeared, the data are measured 3 times at different positions in each area, and the measurement results are expressed by average values of 3 times.
The specific detection results are shown in the following table 2:
TABLE 2
As can be seen from the data in the table above,
examples 1-5 human body moisture content and transdermal water loss were improved, demonstrating that our compositions have a barrier moisturizing and repairing effect, and experiments have demonstrated that the barrier moisturizing and repairing effect is parabolic with composition concentration, i.e., when the composition concentration reaches a certain point, the barrier moisturizing and repairing effect no longer increases with increasing concentration. The results demonstrate the best results of example 5.
Fig. 1 shows the prebiotic composition of example 5 subjected to a cell scratch test to verify cell repair. From the left and right graph changes, the scratch is reduced, and the cells are well repaired.
In order to apply the prebiotic composition to the face of a subject and verify the skin barrier regulation effect of the prebiotic composition on skin microorganisms, a specific effect graph is shown in fig. 2, the effect of the sensitive skin volunteers is evaluated by using example 5, and the results show that the composition has the effects of moisturizing, repairing barriers and improving the skin of the volunteers.
(3) Detection of content of hyaluronidase inhibition
The detection method for inhibiting the content of hyaluronidase comprises the following steps: taking 0.1mL of CaCI, reacting the solution (0.25mmol/L) and 0.5mL of hyaluronidase solution (100U/mL) in water bath at 37 ℃ for 20 min; adding 0.5mL of the composition, and keeping the temperature for 20 min; then adding 0.5mL of sodium hyaluronate solution (0.5g/L), reacting for 30min, taking out and cooling for 5 min; adding 0.1mL of sodium hydroxide solution (0.4mol/L) and 0.5mL of acetylacetone solution (3.5mL of acetylacetone dissolved in 5mL of 1.0mol/L sodium carbonate solution), boiling in water bath for 15min, and immediately transferring to ice water bath for 5 min; 1mL of Ellisib reagent (0.8g p-dimethylaminobenzaldehyde dissolved in 15mL concentrated hydrochloric acid and 15mL absolute ethanol) was added dropwise, and diluted with 3mL absolute ethanol, and the mixture was left at room temperature for 20min to develop color, and the absorbance was measured at 540 nm.
The results are shown in table 3:
TABLE 3
| Serial number | Hyaluronidase inhibition% | Hyaluronidase inhibition% | |
| Example 1 | 90.2％ | Comparative example 1 | 0.2％ |
| Example 2 | 69.3％ | Comparative example 2 | 60.1％ |
| Example 3 | 88.7％ | Comparative example 3 | 62.4％ |
| Example 4 | 75.4％ | Comparative example 4 | 59.6％ |
| Example 5 | 89.2％ |
From the data in the table above, it can be seen that the composition has an inhibitory effect on hyaluronidase, which is beneficial to the production of hyaluronic acid.
It should be understood that the above-described embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. Any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the claims of the present invention.
Claims (10)
1. The prebiotic composition is characterized by comprising, by mass, 5-15 parts of a dendrobium officinale extract, 0.5-2 parts of ribose and 0.1-0.5 part of β glucan.
2. The prebiotic composition of claim 1, which comprises the following components, by mass, 8-12 parts of dendrobium officinale extract, 0.8-1.2 parts of ribose and 0.1-0.3 part of β glucan.
3. The prebiotic composition of claim 2, which comprises the following components, by mass, 10 parts of dendrobium officinale extract, 1 part of ribose and 0.2 part of β glucan.
4. The prebiotic composition of claim 1, wherein the dendrobium officinale polysaccharide in the dendrobium officinale extract is 20-30% by mass.
5. The prebiotic composition of claim 4, wherein the dendrobium officinale polysaccharide content of the dendrobium officinale extract is 25% by weight.
6. Use of prebiotic composition according to any one of claims 1 to 5 in the preparation of a formulation for the regulation of skin homeostasis.
7. Use of the prebiotic composition of any one of claims 1 to 5 in the preparation of a formulation having the efficacy of inhibiting the proliferation of staphylococcus aureus and escherichia coli and promoting the proliferation of staphylococcus epidermidis and bifidobacterium.
8. Use of the prebiotic composition of any one of claims 1 to 5 in the manufacture of a formulation effective to increase the level of hyaluronic acid in skin.
9. A preparation with the effect of regulating the ecological balance of skin, which is characterized by comprising the prebiotic composition of any one of claims 1-5 and other auxiliary materials acceptable for the preparation.
10. The preparation with skin ecological balance regulating effect according to claim 9, wherein the preparation comprises cosmetic and external pharmaceutical preparations, and the dosage forms of the preparation comprise lotions, creams and sprays.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911046600.0A CN110755345A (en) | 2019-10-30 | 2019-10-30 | Prebiotic composition and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911046600.0A CN110755345A (en) | 2019-10-30 | 2019-10-30 | Prebiotic composition and application thereof |
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| CN110755345A true CN110755345A (en) | 2020-02-07 |
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| CN112043645A (en) * | 2020-10-06 | 2020-12-08 | 青岛姿之妍化妆品科技有限责任公司 | Skin care composition |
| CN112156051A (en) * | 2020-08-24 | 2021-01-01 | 四川康养健生物科技有限公司 | Biomass graphene mask and preparation method thereof |
| CN112294719A (en) * | 2020-11-04 | 2021-02-02 | 嘉文丽（福建）化妆品有限公司 | Prebiotic composition for repairing skin barrier and application of prebiotic composition in cosmetics |
| CN112516214A (en) * | 2020-12-20 | 2021-03-19 | 江西兼济堂农业开发有限公司 | Antibacterial and anti-inflammatory composition and preparation method and application thereof |
| CN113397999A (en) * | 2020-03-16 | 2021-09-17 | 无限极（中国）有限公司 | Polysaccharide composition with skin barrier repairing function and application thereof |
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| FR3135899A1 (en) | 2022-03-23 | 2023-12-01 | Basf Beauty Care Solutions France Sas | Cosmetic or dermatological use of a Dendrobium officinale extract to maintain and/or increase the thickness of the skin |
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| CN113397999A (en) * | 2020-03-16 | 2021-09-17 | 无限极（中国）有限公司 | Polysaccharide composition with skin barrier repairing function and application thereof |
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| CN112156051A (en) * | 2020-08-24 | 2021-01-01 | 四川康养健生物科技有限公司 | Biomass graphene mask and preparation method thereof |
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| CN112294719A (en) * | 2020-11-04 | 2021-02-02 | 嘉文丽（福建）化妆品有限公司 | Prebiotic composition for repairing skin barrier and application of prebiotic composition in cosmetics |
| CN112516214A (en) * | 2020-12-20 | 2021-03-19 | 江西兼济堂农业开发有限公司 | Antibacterial and anti-inflammatory composition and preparation method and application thereof |
| WO2023180661A1 (en) | 2022-03-23 | 2023-09-28 | Basf Beauty Care Solutions France Sas | Cosmetic or dermatological use of an extract of dendrobium officinale for maintaining and/or increasing the thickness of the skin |
| FR3135899A1 (en) | 2022-03-23 | 2023-12-01 | Basf Beauty Care Solutions France Sas | Cosmetic or dermatological use of a Dendrobium officinale extract to maintain and/or increase the thickness of the skin |
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