CN107648066A - A kind of anti-aging skin-active shin moisturizer containing growth factor and preparation method thereof - Google Patents
A kind of anti-aging skin-active shin moisturizer containing growth factor and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/494—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with more than one nitrogen as the only hetero atom
- A61K8/4953—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with more than one nitrogen as the only hetero atom containing pyrimidine ring derivatives, e.g. minoxidil
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/4973—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom
- A61K8/498—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom having 6-membered rings or their condensed derivatives, e.g. coumarin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/65—Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
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Abstract
The present invention relates to a kind of anti-aging skin-active shin moisturizer containing growth factor and preparation method thereof, the shin moisturizer includes following component and its 50~70 parts of 0.5~4 part of parts by weight Argireline, 0.1~2 part of carnosine, 0.5~3 part of Ectoin, 0.00001~0.0005 part of recombinant human epidermal growth factor, 0.00001~0.0005 part of recombination human basic fibroblast growth factor, 0.05~1 part of naringenin, 1~4 part of N acetylglucosamines, 0.01~1 part of preservative, 4~8 parts of glycerine, 1~3 part of panthenyl triacetate, 2~6 parts of keratan sulfate oligosaccharide and water.Each composition collocation of shin moisturizer provided by the invention is reasonable, matches science, plays the effect of Synergistic, has the effect of prominent moisturizing, delay skin aging.
Description
Technical field
The present invention relates to cosmetic technical field, and in particular to a kind of anti-aging skin-active shin moisturizer containing growth factor and its
Preparation method.
Background technology
Skin aging is the skin senescence phenomenon as caused by natural cause or non-natural factor.Skin histology day after people's birth
Reach increasingly, function is gradually active.The growth stage of skin histology typically ends at 25 years old or so, enters simultaneously with aging from hereafter growth
OK, elastic fibers of skin is gradually thicker, and 40~50 the beginning of the year old phase, the aging of skin is slowly obvious.The aging of skin be it is a kind of from
Right rule, anti-aging is eternal beauty topic, although we can not prevent the natural aging of skin, can by science and
Effective measures, change the aging state of skin.
Traditional skin nursing is limited only to oil film covering and keeps the physical methods such as moisture, and beauty and skin care theory now
Start to turn to the nursing of cellular level, one bionical cosmetics of forth generation cosmetics (it is manufactured with biotechnology similar with organization of human body,
And the cosmetics of the biological essence substance containing high-affinity) arise at the historic moment, people also gradually recognize biological cosmetics to cosmetic
Product quality brings the change of essence.Biological cosmetics refer to cosmetics made from applied bioengineering technology and its product,
Its main component biologically active polypeptide is largely Porcine HGF, although its addition is atomic, bioactivity is high, right
Various kinds of cell physiological function and metabolic activity play biological regulation effect, can effectively facilitate the function of subcutaneous collagen cell, accelerate
The growth of skin collagen cell, cell is set to accelerate secretion collagen, so as to have the function that crease-resistant and anti-aging.Will at present
EGF (EGF), basic fibroblast growth factor (bFGF), acid fibroblast growth factor (aFGF) etc.
Applied to biological beauty product is prepared, such as：The more vigor emulsions of long-drawn-out Lay of Japanese Shiseido contain epithelical cell growth factor and three
Weight amino acid repair composition, moisturizing, brilliant white, Cucumber Extract, promote the synthesis of collagen, can be effectively improved skin drying,
The problems such as low and deep and wrinkle, while protect skin, extraneous harmful substance is obstructed, make skin youth, brilliant white, gloss and flexible.
And for example：Japanese DHC efficient daily renewal cream contains polypeptide EGF, and anti-aging vitamin, mineral matter and ammonia
The compositions such as base acid, wrinkle can be resisted, prevents skin from following the string, Cucumber Extract, promote the metabolism of cell, strengthen skin bullet
Property and gloss.
Although existing corresponding product occurs on the market, because cell factor is substantially biologically active polypeptide, easily by
Environment influences and inactivated.Prior art is more to be made freeze-dried powder use by Porcine HGF, although freeze-dried powder can be preserved effectively carefully
The activity of intracellular cytokine, but specific skin-protection product is made in cell factor just needs to face stability when cell factor is solution shape
Poor problem.This needs technical staff the formulation and protective agent of selection, to keep factor active according to products characteristics, ensure production
Quality, if the A of Publication No. CN 102988200 Chinese patent application " liposome face nourishing skin care products " is by EGF
(EGF), fat is made in the composition such as human stem cell growth (SCGF), hyaluronic acid, metallothionein, collagen, ursin
Plastid emulsion, by encapsulating above-mentioned material, the stability and its bioavilability of growth factor are improved, achieves preferable shield
Skin effect.A kind of and for example A of Publication No. CN 103860392 Chinese patent application " hydrophobic solid containing EGF
Hydrophobic solid microballoon is made in EGF by microballoon and its preparation method and application ", then containing EGF
Hydrophobic solid microballoon is added in various emulsions, paste or white shape cosmetics as functional additive.Above-mentioned scheme can solve
The problem of growth factor stability, but preparation technology is complex, and cost is higher.
Therefore, it is necessary to provide, a kind of stability is good, has the shin moisturizer containing growth factor of preferable anti-ageing skin-active effect,
To meet the market demand.
The content of the invention
To solve the problems, such as that prior art is present, it is an object of the invention to provide a kind of anti-aging work containing growth factor
Protecting skin liquid and preparation method thereof, each composition of the shin moisturizer is mutually coordinated, and anti-aging effects are notable, no skin irritation, safety
Property it is high, property is stable.
Technical scheme is as follows：
A kind of anti-aging skin-active shin moisturizer containing growth factor, including following component and its parts by weight：Argireline 0.5~
4 parts, 0.1~2 part of carnosine, 0.5~3 part of Ectoin, 0.00001~0.0005 part of recombinant human epidermal growth factor, again
0.00001~0.0005 part of human alkaline fibroblast growth factor of group, 0.05~1 part of naringenin, N-Acetyl-D-glucosamine 1~
50~70 parts of 4 parts, 0.01~1 part of preservative, 4~8 parts of glycerine and water.
Preferably, the anti-aging skin-active shin moisturizer containing growth factor includes following component and its parts by weight：Six
Win 1~2 part of peptide, 0.5~1 part of carnosine, 1~2 part of Ectoin, recombinant human epidermal growth factor 0.0001~0.0005
Part, 0.0001~0.0005 part of recombination human basic fibroblast growth factor, 0.1~1 part of naringenin, N-Acetyl-D-glucosamine
50~60 parts of 2~4 parts, 0.1~1 part of preservative, 6~8 parts of glycerine and water.
Further, the anti-aging skin-active shin moisturizer containing growth factor also includes 1~3 part of panthenyl triacetate.
Further, the anti-aging skin-active shin moisturizer containing growth factor also includes keratan sulfate oligosaccharide 2~6
Part.
It is highly preferred that the anti-aging skin-active shin moisturizer containing growth factor includes following component and its parts by weight：
1~2 part of Argireline, 0.5~1 part of carnosine, 1~2 part of Ectoin, recombinant human epidermal growth factor 0.0001~0.0005
Part, 0.0001~0.0005 part of recombination human basic fibroblast growth factor, 0.1~1 part of naringenin, N-Acetyl-D-glucosamine
2~4 parts, 1~3 part of panthenyl triacetate, 2~6 parts of keratan sulfate oligosaccharide, 0.1~1 part of preservative, 6~8 parts of glycerine and
50~60 parts of water.
Specifically, above-mentioned keratan sulfate oligosaccharide is keratan sulfate disaccharides and keratan sulfate trisaccharide with 1:0.2
~0.4 mass ratio composition.
Preferably, described keratan sulfate oligosaccharide is keratan sulfate disaccharides and keratan sulfate trisaccharide with 1:0.25
Mass ratio composition.
Specifically, described preservative is phenyllactic acid, benzyl carbinol or Sensiva SC50.
In addition, the present invention also provides a kind of preparation method of the described anti-aging skin-active shin moisturizer containing growth factor, its
Comprise the following steps：
Naringenin, glycerine, Ectoin are weighed, water is added after mixing, 75~85 DEG C is stirred and heated to, treats material dissolution
Afterwards, 35~40 DEG C are cooled to, sequentially adds Argireline, carnosine, recombinant human epidermal growth factor, recombination human basic fibroblast
Porcine HGF, N-Acetyl-D-glucosamine, preservative, discharge, produce after stirring.
Further, the preparation method of the above-mentioned anti-aging skin-active shin moisturizer containing growth factor also includes adding
The step of panthenyl triacetate and keratan sulfate oligosaccharide.
In the shin moisturizer of the present invention, Ectoin also known as tetrahydrochysene methylpyrimidine carboxylic acid, a kind of high Halophiles is derived from
In vivo the effect of composition, be cutaneous immunisation conditioning agent, can reduce because dry and ultraviolet Irradiation on Skin injury, have very well
Moisturizing, maintenance, resist microgroove the effect of.By itself and recombinant human epidermal growth factor and recombination human basic fibroblast cell
Growth factor compounds, and can promote the regeneration of skin collagen and the restructuring of fibr tissue, strengthens skin elasticity, and it is quick to reduce skin
Sensitivity, thick face, flush, the capillary of expansion can be made to attenuate and shoal, and reduce pigmentation, go out cutaneous manifestations tender white
Have no time.Three cooperates with other skin conditioning agents in naringenin and system, such as Argireline, carnosine, has more excellent resist
Wrinkle effect is gone in aging, makes skin compact flexible, profile is apparent.
However, due to containing more polypeptides matter, its less stable, in normal temperature bar in shin moisturizer system of the present invention
Under part, some physical and chemical changes easily occur for the aqueous solution, cause the hydrophobic surface of polypeptides matter to accumulate increase and occur poly-
Conjunction behavior, dissolubility reduction is ultimately resulted in, activity is reduced or disappeared, the effect of influenceing shin moisturizer.Although the inventors discovered that according to
The effect of Ke Duoyin addition can effectively improve moisturizing, the delay skin aging of shin moisturizer of the present invention, but because Ectoin contains
There is free carboxyl, influence the ionic strength of shin moisturizer system, obtained shin moisturizer bin stability is deteriorated, store at normal temperatures
After depositing one month, engender the clustering phenomena of polypeptide and be in muddy shape.Surprisingly, the inventors discovered that protecting
Individually compounding panthenyl triacetate or keratan sulfate oligosaccharide, or compound panthenyl triacetate and keratosulfate simultaneously in skin liquid
It plain oligosaccharide, can effectively suppress the clustering phenomena of polypeptide, significantly improve the stability of shin moisturizer storage at normal temperature, make shin moisturizer normal
Temperature storage still keeps homogeneous clear and bright shape for 24 months, moisturizing, the significant effect of anti-aging, especially to compound panthenyl triacetate simultaneously
With the optimal stability of keratan sulfate oligosaccharide, unexpected technique effect is achieved.
Contain more hydroxyl in described panthenyl triacetate molecular structure, have stronger anti-oxidant and stablize pH value
Effect, is effective against influence of the free carboxy to peptide material of Ectoin；And by keratan sulfate disaccharides and sulfuric acid angle
Quality trisaccharide, than the keratan sulfate oligosaccharide formed, can be reduced the noncovalent interaction power between polypeptide, suppressed with certain mass
It is intermolecular to form condensate by hydrophobic forces, so as to improve the stability of peptide material in aqueous.
Keratan sulfate oligosaccharide provided by the invention can be commercially available prod, or made products, its preparation side
Method refers to Publication No. CN 1174557A Chinese patent " keratan sulfate oligosaccharide fraction and the medicament containing the fraction ", tool
Body is：Keratan sulfate 50g is taken, is dissolved in 300ml 0.1M acetate buffer solutions (pH6.0), adds-β-N- acetyl in 25U
Base glucosaminidase type sulfuric acid digestive enzyme, degrade 24h at 37 DEG C.After reaction terminates, the ethanol of 2 times of volumes is added, is stirred,
Placed for 1 night at room temperature, 15min is centrifuged under 4000rpm, take supernatant (supernatant A).300ml distilled water is added into precipitation, is carried out
Dissolving, the ethanol of 3 times of amounts is added, stirring, placed at room temperature for 1 night, 15min is centrifuged under 4000rpm, take supernatant (supernatant B).Will
Supernatant A and supernatant B mixing, is concentrated under reduced pressure, and using Bio-Gel-P-2 posts (3.6 ╳ 134cm), using distilled water as solvent, carries out
Gel filtration, freeze-drying, obtained freeze-dried powder is finally separated, collect keratosulfate respectively using ion-exchange chromatography
The fraction of plain disaccharides, keratan sulfate trisaccharide, mix and produce in proportion after drying.
Compared with prior art, advantage of the invention is that：
(1) each composition collocation of shin moisturizer provided by the invention is reasonable, matches science, plays the effect of Synergistic, efficiently
Moisturizing, reply skin jade-like stone profit gloss, reduce pigmentation, make cutaneous manifestations go out it is tender have no time in vain, for more single aging ingredient,
The effect of with prominent moisturizing, delay skin aging.
(2) shin moisturizer property provided by the invention is gentle, and safely and effectively, and stability is high, in storage at normal temperature 24 months still
The homogeneous clear and bright shape of holding, skin effect are notable.
Embodiment
The present invention is further described below by way of embodiment, but the present invention is not limited only to following examples.
The keratan sulfate oligosaccharide of embodiment 1 prepares and analysis
(1) keratan sulfate 100g is taken, is dissolved in 600ml 0.1M acetate buffer solutions (pH6.0), adds 50U mixing
(keratanase II and N-acetyl-β-D-glucosaminidase are with 1 for enzyme:1 mass ratio composition), degrade 24h at 37 DEG C.Instead
After should terminating, the ethanol of 4 times of volumes is added, stirring, placed for 1 night at room temperature, centrifuges 10min at 3,000 rpm, take supernatant
(supernatant A).600ml distilled water is added into precipitation, is dissolved, the ethanol of 6 times of amounts is added, stirring, places 1 at room temperature
At night, 10min is centrifuged under 3000rpm, takes supernatant (supernatant B).Supernatant A and supernatant B is mixed, is concentrated under reduced pressure, uses Bio-Gel-
P-2 posts (3.6 ╳ 134cm), using distilled water as solvent, gel filtration is carried out, filtrate is freeze-dried, obtains freeze-dried powder.
(2) obtained freeze-dried powder is respectively adopted into ion-exchange chromatography to be separated, obtain respectively keratan sulfate disaccharides,
Keratan sulfate trisaccharide, keratan sulfate tetrose, keratan sulfate pentasaccharides fraction, after freeze-dried, using high-efficient liquid phase color
Spectrum is analyzed, and detects the content of each composition.As a result see the table below shown in 1：
The content of each oligosaccharide in oligosaccharide made from the 100g keratan sulfates of table 1
| Form (content %) | Keratan sulfate disaccharides | Keratan sulfate trisaccharide | Keratan sulfate tetrose | Keratan sulfate pentasaccharides |
| A | 41.8 | 26.2 | 15.0 | 7.5 |
The preparation of the shin moisturizer of embodiment 2
Anti-aging skin-active shin moisturizer of the embodiment 2 containing growth factor includes following component and its parts by weight：Argireline 2
Part, 1 part of carnosine, 1.5 parts of Ectoin, 0.00025 part of recombinant human epidermal growth factor, recombination human basic fibroblast cell
0.0001 part of growth factor, 0.5 part of naringenin, 3 parts of N-Acetyl-D-glucosamine, 1 part of panthenyl triacetate, keratan sulfate oligomerization
55 parts of 5 parts of sugar, 1 part of preservative, 6 parts of glycerine and water；Wherein, keratan sulfate oligosaccharide is keratosulfate made from embodiment 1
Plain disaccharides and keratan sulfate trisaccharide is with 1:0.25 mass ratio composition.
Preparation method：
Naringenin, glycerine, Ectoin, panthenyl triacetate, keratan sulfate oligosaccharide are weighed, water is added after mixing,
80 DEG C are stirred and heated to, after material dissolution, is cooled to 40 DEG C, sequentially adds Argireline, carnosine, the life of recombinant human epidermal cell
The long factor, recombination human basic fibroblast growth factor, N-Acetyl-D-glucosamine, preservative, discharged after stirring, i.e.,
.
The preparation of the shin moisturizer of embodiment 3
Anti-aging skin-active shin moisturizer of the embodiment 3 containing growth factor includes following component and its parts by weight：Argireline 1
Part, 0.5 part of carnosine, 1 part of Ectoin, 0.0003 part of recombinant human epidermal growth factor, recombination human basic fibroblast cell
0.0002 part of growth factor, 1 part of naringenin, 3 parts of N-Acetyl-D-glucosamine, 2 parts of panthenyl triacetate, keratan sulfate oligosaccharide
55 parts of 4 parts, 1 part of preservative, 8 parts of glycerine and water；Wherein, keratan sulfate oligosaccharide is keratan sulfate made from embodiment 1
Disaccharides and keratan sulfate trisaccharide are with 1:0.25 mass ratio composition.
Preparation method reference implementation example 2.
The preparation of the shin moisturizer of embodiment 4
Anti-aging skin-active shin moisturizer of the embodiment 4 containing growth factor includes following component and its parts by weight：Argireline 2
Part, 1 part of carnosine, 1.5 parts of Ectoin, 0.00025 part of recombinant human epidermal growth factor, recombination human basic fibroblast cell
0.0001 part of growth factor, 0.5 part of naringenin, 3 parts of N-Acetyl-D-glucosamine, 1 part of panthenyl triacetate, keratan sulfate oligomerization
55 parts of 5 parts of sugar, 1 part of preservative, 6 parts of glycerine and water；Wherein, keratan sulfate oligosaccharide is keratosulfate made from embodiment 1
Plain trisaccharide and keratan sulfate tetrose is with 1:0.25 mass ratio composition.
Preparation method reference implementation example 2.
The preparation of the shin moisturizer of embodiment 5
Anti-aging skin-active shin moisturizer of the embodiment 5 containing growth factor includes following component and its parts by weight：Argireline 2
Part, 1 part of carnosine, 1.5 parts of Ectoin, 0.00025 part of recombinant human epidermal growth factor, recombination human basic fibroblast cell
0.0001 part of growth factor, 0.5 part of naringenin, 3 parts of N-Acetyl-D-glucosamine, 1 part of panthenyl triacetate, keratan sulfate oligomerization
55 parts of 5 parts of sugar, 1 part of preservative, 6 parts of glycerine and water；Wherein, keratan sulfate oligosaccharide is keratosulfate made from embodiment 1
Plain tetrose and keratan sulfate pentasaccharides is with 1:0.25 mass ratio composition.
Preparation method reference implementation example 2.
The preparation of the shin moisturizer of comparative example 1
The difference of anti-aging skin-active shin moisturizer of the comparative example 1 containing growth factor and embodiment 2 be to be free of according to gram more than
Cause, it includes following component and its parts by weight：2 parts of Argireline, 1 part of carnosine, recombinant human epidermal growth factor 0.00025
Part, 0.0001 part of recombination human basic fibroblast growth factor, 0.5 part of naringenin, 3 parts of N-Acetyl-D-glucosamine, panthenol three
55 parts of 1 part of acetic acid esters, 5 parts of keratan sulfate oligosaccharide, 1 part of preservative, 6 parts of glycerine and water；Wherein, keratan sulfate oligosaccharide
It is keratan sulfate disaccharides made from embodiment 1 and keratan sulfate trisaccharide with 1:0.25 mass ratio composition.
Preparation method reference implementation example 2.
The preparation of the shin moisturizer of comparative example 2
Anti-aging skin-active shin moisturizer of the comparative example 2 containing growth factor and the difference of embodiment 2 are to be free of panthenol three
Acetic acid esters, it includes following component and its parts by weight：2 parts of Argireline, 1 part of carnosine, 1.5 parts of Ectoin, recombinant human epidermal
0.00025 part of Porcine HGF, 0.0001 part of recombination human basic fibroblast growth factor, 0.5 part of naringenin, N- acetyl
55 parts of 3 parts of gucosamine, 5 parts of keratan sulfate oligosaccharide, 1 part of preservative, 6 parts of glycerine and water；Wherein, keratan sulfate oligomerization
Sugar is keratan sulfate disaccharides made from embodiment 1 and keratan sulfate trisaccharide with 1:0.25 mass ratio composition.
Preparation method reference implementation example 2.
The preparation of the shin moisturizer of comparative example 3
The difference of anti-aging skin-active shin moisturizer and embodiment 2 of the comparative example 3 containing growth factor is to replace with trehalose
Keratan sulfate oligosaccharide is changed, it includes following component and its parts by weight：2 parts of Argireline, 1 part of carnosine, Ectoin 1.5
Part, 0.00025 part of recombinant human epidermal growth factor, 0.0001 part of recombination human basic fibroblast growth factor, shaddock ped
0.5 part of element, 3 parts of N-Acetyl-D-glucosamine, 1 part of panthenyl triacetate, 5 parts of trehalose, 1 part of preservative, 6 parts of glycerine and water 55
Part.
Preparation method reference implementation example 2.
The preparation of the shin moisturizer of comparative example 4
Anti-aging skin-active shin moisturizer of the comparative example 4 containing growth factor and the difference of embodiment 2 are to be free of panthenol three
Acetic acid esters and keratan sulfate oligosaccharide, it includes following component and its parts by weight：2 parts of Argireline, 1 part of carnosine, according to gram more than
Because 1.5 parts, 0.00025 part of recombinant human epidermal growth factor, 0.0001 part of recombination human basic fibroblast growth factor,
55 parts of 0.5 part of naringenin, 3 parts of N-Acetyl-D-glucosamine, 1 part of preservative, 6 parts of glycerine and water.
Preparation method reference implementation example 2.
Test example one, skin irritation test
160 female volunteers are selected, 80 male volunteers, are divided into 8 groups, respectively embodiment 2-5 groups, comparative example 1-
4 groups, every group of 20 women and 10 males are corresponding to give shin moisturizer made from 2-5 of the embodiment of the present invention, comparative example 1-4, with note
Emitter takes 0.03ml samples to be placed in the coyote hole of spot examination adhesive tape, and the spot for being equipped with sample is tried into adhesive tape longitudinal patch since bottom immediately
In on the normal skin of left forearm, while light pressing room to drive away air, and is uniformly distributed trier one by one.Do at experiment position
Good mark, to observe.Every 24 hours patch skins once, paster half an hour after is opened, is examined according to the term of CTFA guides
Look into, carry out 6 patch tests altogether, paste the 6th day after skin terminates, in order to observe delayed response, carried out additional inspection.To last
The result once checked is compared, and as a result see the table below shown in 2 and 3.
The skin irritation test standard of perfection of table 2 is judged
| Grade | Symbol | Standard of perfection |
| 0 | - | Negative reaction；It is non-stimulated, without erythema |
| 1 | ± | Suspicious reaction：Slight erythema |
| 2 | + | Weakly positive is reacted：Erythema |
| 3 | ++ | Strong positive reaction：Erythema, papule, blister |
| 4 | +++ | Extremely strong positive reaction：Serious edema, bulla |
The skin irritation test result of table 3
As a result show, the no positive reaction of shin moisturizer made from 2-5 of the embodiment of the present invention and comparative example 1-4 occurs, skin
Excitant grade is 0 grade, illustrates that shin moisturizer provided by the invention is non-stimulated to skin, product safety, gentle.
Test example two, the experiment of shin moisturizer using effect
160 female volunteers are selected, are divided into 8 groups, respectively embodiment 2-5 groups, comparative example 1-4 groups, it is every group 20, right
Shin moisturizer made from 2-5 of the embodiment of the present invention, comparative example 1-4 should be given, it is desirable to which early, middle and late use is once described for each person every day
Shin moisturizer, application method is applied to face in right amount to take, gently loops up to shin moisturizer fully absorbs.Every user is entered
The regular follow-up investigation of row, using skinanalysis apparatus (Derma-Expert MC760), every 10 days are to test once in a stage, test
Position is both sides cheek.Recover natural elasticity, improvement wrinkle of skin and coarse, raising to skin to moisten the several respects such as intensity and carry out
Test and carry out data analysis, as a result see the table below shown in 4 and 5.
The shin moisturizer of table 4 improves skin elasticity and reduces the evaluation result of skin friction power
Note：D0For the 0th day data measured, D10The 10th day data measured, D20For the 20th day data measured.
The shin moisturizer of table 5 improves the evaluation result of skin water content
Note：D0For the 0th day data measured, D10The 10th day data measured, D20For the 20th day data measured.
As a result show, shin moisturizer made from the embodiment of the present invention 2 and 3 is to improving skin elasticity and skin moisture, and drop
Low skin friction power has a significant effect, better than shin moisturizer made from embodiment 4 and 5 and comparative example 1-4.By embodiment 4
Understood with 5, keratan sulfate oligosaccharide is the mixture of keratan sulfate trisaccharide and tetrose, or for keratan sulfate tetrose and
The mixture of pentasaccharides, weaken the using effect of shin moisturizer.From comparative example 1, shin moisturizer is free of Ectoin, and it uses effect
Fruit significantly reduces, and shows that Ectoin recovers natural elasticity, improvement wrinkle of skin and coarse, the moist intensity of raising to skin and had
Important function.From comparative example 2, shin moisturizer is free of panthenyl triacetate, and its using effect slightly reduces, and shows the second of panthenol three
Acid esters can influence the effect of shin moisturizer to a certain extent.From comparative example 3, keratan sulfate oligomerization is replaced with trehalose
Sugar, the using effect of shin moisturizer reduce, and show keratan sulfate oligosaccharide composition compounding each with shin moisturizer of the present invention more preferably, more have
Beneficial to the skin effect for playing shin moisturizer.From comparative example 4, shin moisturizer is free of panthenyl triacetate and keratan sulfate simultaneously
Oligosaccharide, the using effect of shin moisturizer reduce, and show that the interaction of both panthenyl triacetate and keratan sulfate oligosaccharide has
Help improve skin effect.
Test example three, Oxidation Resistance Test
Free radical is that a kind of property is active, has a chemical substance of extremely strong oxidability, in body it is caused it is a large amount of freely
Base can also cause to damage to skin, accelerate the formation of color spot, melanin.DPPH is a kind of long-life free radical of stabilization, when having
In the presence of free radical scavenger, weaken DPPH light absorbs due to being matched with its single electron, evaluate material whereby and remove freely
The ability of base, so as to evaluate its oxidation resistance.The present invention determines absorbance, detection is real by DPPH free radical neutralization tests
Clearance rate of the shin moisturizer made from a 2-5, comparative example 1-4 to DPPH free radicals is applied, to evaluate shin moisturizer produced by the present invention
Antioxidant effect, as a result it see the table below shown in 6.
Clearance rate of each group shin moisturizer of table 6 to DPPH free radicals
| Group | Clearance rate % |
| Embodiment 2 | 81.26 |
| Embodiment 3 | 80.48 |
| Embodiment 4 | 79.54 |
| Embodiment 5 | 79.32 |
| Comparative example 1 | 75.08 |
| Comparative example 2 | 72.67 |
| Comparative example 3 | 75.44 |
| Comparative example 4 | 70.25 |
From upper table 6, shin moisturizer provided by the invention can effectively remove DPPH free radicals, show activity shield of the invention
Skin composition each component compounding is reasonable, mutually coordinated, has preferable antioxidant effect, suppresses the formation of color spot, melanin.
Test example four, stability test
(1) accelerated test：2-5 of the embodiment of the present invention, comparative example 1-4 obtained shin moisturizer are taken, carries out high and low temperature storage
Stability test is investigated, experimental condition：1) thimble test：Placed 1 week in 60 DEG C of electro-heating standing-temperature cultivators, recovery room
Observed after temperature；2) low-temperature stability is tested：Deposited 1 week at 2 DEG C, observe its stability, it is as a result as shown in table 7 below.
The each group shin moisturizer accelerated test Detection of Stability result of table 7
As a result showing, shin moisturizer produced by the present invention has preferable stability, is placed one week in high temperature and cryogenic conditions,
Keep homogeneous clear and bright shape.From embodiment 5, keratan sulfate oligosaccharide is the mixing of keratan sulfate tetrose and pentasaccharides
Thing, the high-temperature stability tool of shin moisturizer is had a certain impact, is placed one week in 60 DEG C of hot conditions, deposited phenomenon occurs, but
It is not layered；And one week is placed under cryogenic, shin moisturizer is relatively stable, keeps homogeneous clear and bright shape.Can by comparative example 1
Know, shin moisturizer is free of Ectoin, no matter in high temperature or cryogenic conditions, keeps preferable stability.Can by comparative example 2
Know, shin moisturizer is free of panthenyl triacetate, and its bad stability is especially more unstable to hot conditions.Can by comparative example 3
Know, keratan sulfate oligosaccharide is replaced with trehalose, the stability of shin moisturizer does not improve, shows keratan sulfate oligosaccharide
It is preferable with the compounding effect of panthenyl triacetate, be advantageous to improve stability.From comparative example 4, shin moisturizer is simultaneously without general
Alcohol triacetate and keratan sulfate oligosaccharide, the stability of shin moisturizer is worst, shows panthenyl triacetate and keratan sulfate
The interaction of both oligosaccharides plays an important roll to the stability for improving shin moisturizer.
(2) normal temperature long term test：2-5 of the embodiment of the present invention, comparative example 1-4 obtained shin moisturizer are taken, carries out normal temperature storage
Deposit stability test investigation, experimental condition：23~28 DEG C are placed at, relative humidity is 30~60%, observes its stability, as a result
It is as shown in table 8 below.
The each group shin moisturizer normal temperature long term test Detection of Stability result of table 8
From upper table 8, panthenyl triacetate and keratan sulfate oligosaccharide have weight to the long-time stability of shin moisturizer
The effect wanted, both interact, and greatly improve the bin stability of shin moisturizer, extend the term of validity that shin moisturizer uses.
It the above is only the preferred embodiment of the present invention, it is noted that above-mentioned preferred embodiment is not construed as pair
The limitation of the present invention, protection scope of the present invention should be defined by claim limited range.For the art
For those of ordinary skill, without departing from the spirit and scope of the present invention, some improvements and modifications can also be made, these change
Enter and retouch and also should be regarded as protection scope of the present invention.
Claims (10)
1. a kind of anti-aging skin-active shin moisturizer containing growth factor, it is characterised in that including following component and its parts by weight：Six
0.5~4 part of peptide of victory, 0.1~2 part of carnosine, 0.5~3 part of Ectoin, recombinant human epidermal growth factor 0.00001~
0.0005 part, 0.00001~0.0005 part of recombination human basic fibroblast growth factor, 0.05~1 part of naringenin, N- acetyl
50~70 parts of 1~4 part of gucosamine, 0.01~1 part of preservative, 4~8 parts of glycerine and water.
2. the anti-aging skin-active shin moisturizer according to claim 1 containing growth factor, it is characterised in that including following component
And its parts by weight：1~2 part of Argireline, 0.5~1 part of carnosine, 1~2 part of Ectoin, recombinant human epidermal growth factor
0.0001~0.0005 part, 0.0001~0.0005 part of recombination human basic fibroblast growth factor, 0.1~1 part of naringenin,
50~60 parts of 2~4 parts of N-Acetyl-D-glucosamine, 0.1~1 part of preservative, 6~8 parts of glycerine and water.
3. the anti-aging skin-active shin moisturizer according to claim 1 or 2 containing growth factor, it is characterised in that also including general
1~3 part of alcohol triacetate.
4. the anti-aging skin-active shin moisturizer according to claim 1 or 2 containing growth factor, it is characterised in that also including sulphur
2~6 parts of tamarind quality oligosaccharide.
5. the anti-aging skin-active shin moisturizer according to claim 4 containing growth factor, it is characterised in that including following component
And its parts by weight：1~2 part of Argireline, 0.5~1 part of carnosine, 1~2 part of Ectoin, recombinant human epidermal growth factor
0.0001~0.0005 part, 0.0001~0.0005 part of recombination human basic fibroblast growth factor, 0.1~1 part of naringenin,
2~4 parts of N-Acetyl-D-glucosamine, 1~3 part of panthenyl triacetate, 2~6 parts of keratan sulfate oligosaccharide, preservative 0.1~1
50~60 parts of part, 6~8 parts of glycerine and water.
6. the anti-aging skin-active shin moisturizer according to claim 4 containing growth factor, it is characterised in that described sulfuric acid angle
Quality oligosaccharide is keratan sulfate disaccharides and keratan sulfate trisaccharide with 1:0.2~0.4 mass ratio composition.
7. the anti-aging skin-active shin moisturizer according to claim 6 containing growth factor, it is characterised in that described sulfuric acid angle
Quality oligosaccharide is keratan sulfate disaccharides and keratan sulfate trisaccharide with 1:0.25 mass ratio composition.
8. the anti-aging skin-active shin moisturizer according to claim 1 or 2 containing growth factor, it is characterised in that described is anti-
Rotten agent is phenyllactic acid, benzyl carbinol or Sensiva SC50.
9. the preparation method of the anti-aging skin-active shin moisturizer according to claim 1 or 2 containing growth factor, its feature exist
In comprising the following steps：
Naringenin, glycerine, Ectoin are weighed, water is added after mixing, is stirred and heated to 75~85 DEG C, after material dissolution,
35~40 DEG C are cooled to, it is thin to sequentially add Argireline, carnosine, recombinant human epidermal growth factor, recombination human basic fibroblast
The intracellular growth factor, N-Acetyl-D-glucosamine, preservative, discharge, produce after stirring.
10. the preparation method of the anti-aging skin-active shin moisturizer according to claim 9 containing growth factor, it is characterised in that
The step of also including adding panthenyl triacetate and keratan sulfate oligosaccharide.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108888527A (en) * | 2018-09-16 | 2018-11-27 | 美尔健（深圳）生物科技有限公司 | It is a kind of to improve dry skin, sensitive freeze dried powder and preparation method thereof |
| CN112603867A (en) * | 2021-01-04 | 2021-04-06 | 广东名兰化妆品制造有限公司 | Skin membrane repairing essence stock solution and preparation method thereof |
| KR102620622B1 (en) * | 2023-09-08 | 2024-01-04 | 주식회사 제이포셀 | Cosmetic composition containing EGF with improved storage stability. |
-
2017
- 2017-11-15 CN CN201711131393.XA patent/CN107648066A/en not_active Withdrawn
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108888527A (en) * | 2018-09-16 | 2018-11-27 | 美尔健（深圳）生物科技有限公司 | It is a kind of to improve dry skin, sensitive freeze dried powder and preparation method thereof |
| CN112603867A (en) * | 2021-01-04 | 2021-04-06 | 广东名兰化妆品制造有限公司 | Skin membrane repairing essence stock solution and preparation method thereof |
| KR102620622B1 (en) * | 2023-09-08 | 2024-01-04 | 주식회사 제이포셀 | Cosmetic composition containing EGF with improved storage stability. |
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Application publication date: 20180202 |