CN111758856A - Method for producing micro-ecological nutrient solution by using aquatic product processing by-products - Google Patents

Method for producing micro-ecological nutrient solution by using aquatic product processing by-products Download PDF

Info

Publication number
CN111758856A
CN111758856A CN202010664032.7A CN202010664032A CN111758856A CN 111758856 A CN111758856 A CN 111758856A CN 202010664032 A CN202010664032 A CN 202010664032A CN 111758856 A CN111758856 A CN 111758856A
Authority
CN
China
Prior art keywords
nutrient solution
micro
fermentation
treatment
yield
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202010664032.7A
Other languages
Chinese (zh)
Inventor
林斐
林恒贵
林丽钦
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fujian Hengxiang Fishery Co ltd
Original Assignee
Fujian Hengxiang Fishery Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fujian Hengxiang Fishery Co ltd filed Critical Fujian Hengxiang Fishery Co ltd
Priority to CN202010664032.7A priority Critical patent/CN111758856A/en
Publication of CN111758856A publication Critical patent/CN111758856A/en
Pending legal-status Critical Current

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05B—PHOSPHATIC FERTILISERS
    • C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80—Soil conditioners
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00—Fertilisers characterised by their form
    • C05G5/20—Liquid fertilisers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish
    • Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Microbiology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Polymers & Plastics (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Pest Control & Pesticides (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Virology (AREA)
  • Animal Husbandry (AREA)
  • Food Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Botany (AREA)
  • Physiology (AREA)
  • Soil Sciences (AREA)
  • Insects & Arthropods (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Birds (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Fertilizers (AREA)

Abstract

The invention discloses a method for producing a micro-ecological nutrient solution by using aquatic product processing byproducts, belonging to the technical field of fermentation engineering. The method for producing the micro-ecological nutrient solution by using the aquatic product processing by-products disclosed by the invention biologically converts the aquatic product processing by-products into the micro-ecological nutrient solution with high added value through the high-efficiency biological metabolism of the microbial ecological flora, thereby realizing the comprehensive utilization of resources. The produced microecological nutrient solution is directly used as a nutrient of a leaf fertilizer for crops, so that fruits or leaves of the fruits, the vegetables and the like are bright in color, and the yield and the quality are improved.

Description

Method for producing micro-ecological nutrient solution by using aquatic product processing by-products
Technical Field
The invention relates to the technical field of fermentation engineering, in particular to a method for producing a micro-ecological nutrient solution by using aquatic product processing byproducts.
Background
With the development of the aquaculture industry, the aquatic product processing byproducts including fish processing wastes are increasing, and if the aquatic product processing byproducts are not effectively treated, the wastes are easy to decay, generate foul smell and cause serious environmental pollution. Meanwhile, aquatic product processing byproducts such as fish processing waste and the like contain nutrients such as protein, phospholipid, chondroitin, vitamins, calcium and the like, and a large amount of nutrients are wasted while being discarded; the added value of fish processing can be improved by fully utilizing the wastes, and good economic and social benefits are obtained.
Therefore, it is an urgent problem to those skilled in the art to provide a method for producing a micro-ecological nutrient solution using aquatic product processing by-products.
Disclosure of Invention
In view of the above, the invention provides a method for producing a micro-ecological nutrient solution by using aquatic product processing byproducts, which biologically converts the aquatic product processing byproducts into the micro-ecological nutrient solution with high added value through the high-efficiency biological metabolism of microbial ecological flora, thereby realizing the comprehensive utilization of resources.
In order to achieve the purpose, the invention adopts the following technical scheme:
a method for producing a micro-ecological nutrient solution by using aquatic product processing byproducts comprises the following specific steps:
(1) preparation of seed liquid
Activating strains: activating and culturing spore bacteria, yeast and lactobacillus (common strain) under aseptic condition.
A. Activating spore bacteria: the stored spore strain is firstly subjected to streak activation on a beef extract peptone solid culture medium (beef extract 3g/L, peptone 10g/L, sodium chloride 5g/L, agar 20g/L, pH7.0-7.2, sterilization at 121 ℃ for 20min), and the temperature is 35 ℃ for 12-16 h; inoculating activated strain to 250mL (100 mL) triangular flask containing beef extract peptone liquid medium, and shake-culturing at 35 deg.C and 250r/min for 24 hr; then the culture is expanded and activated according to the inoculation amount of 10 percent.
B. Activation of yeast: the method comprises the following steps of firstly, marking and activating a yeast strain on a yeast powder peptone glucose solid culture medium YPD (peptone 20g/L, glucose 20g/L, yeast extract 10g/L, agar 20g/L, sterilizing at 121 ℃ for 20min), and carrying out 28 ℃ and 24 h; inoculating activated strain to 250mL (100 mL) triangular flask containing yeast powder peptone glucose liquid culture medium, and shake-culturing at 28 deg.C and 250r/min for 36 h; then the culture is expanded and activated according to the inoculation amount of 10 percent.
C. Activating lactic acid bacteria: firstly, streaking and activating the stored lactobacillus strains on an MRS solid culture medium (10 g/L of peptone, 8g/L of beef extract, 4g/L of yeast extract, 20g/L of glucose, 2g/L of dipotassium phosphate, 2g/L of diammonium hydrogen citrate, 5g/L of sodium acetate, 0.2g/L of magnesium sulfate, 0.04g/L of manganese sulfate, 801.0mL/L of tween, 20g/L of calcium carbonate, 20g/L of agar, pH 6.5 and high-pressure steam sterilization at 121 ℃ for 20min), and performing high-pressure steam sterilization at 40 ℃ for 24 h; inoculating activated strain to 250mL (loading 100mL) triangular flask containing MRS liquid culture medium, and shake-culturing at 40 deg.C and 250r/min for 40 h; then the culture is expanded and activated according to the inoculation amount of 10 percent.
Preparing seed suspension: and (3) performing amplification culture on the activated spore bacteria, saccharomycetes and lactic acid bacteria according to the ratio of 2: 1: 3, mixing the raw materials in proportion to prepare seed suspension;
③ expanding and culturing seeds: inoculating the prepared seed suspension into a 100L primary seed tank containing a primary seed culture medium with the inoculation amount of 10%, wherein the loading volume of the primary seed culture medium is 65-75%, the stirring speed is 80-100r/min at 30-35 ℃, the ventilation volume is 1L/min, the tank pressure is 0.10-0.13Mpa, and the culture is carried out for 36 h; taking fermentation liquor to detect pH and viable count every 6h to obtain qualified first-grade seed culture solution; continuing expanding culture: inoculating the primary seed culture solution into a 500L secondary seed tank containing a secondary seed culture medium with the inoculation amount of 10%, the loading volume of the secondary seed culture medium is 65-75%, the stirring speed is 90-120r/min at 30-35 ℃, the ventilation volume is 1L/min, the tank pressure is 0.10-0.13Mpa, and continuously culturing for 36 h; taking fermentation liquor to detect pH and viable count every 6h to obtain qualified secondary seed culture solution;
(2) pretreatment of raw materials
Cooking the aquatic product processing by-product according to a solid-to-liquid ratio of 2:1, boiling for 30-45min to obtain leftovers; the main purpose is to kill the mixed bacteria and soften the solid, if the aquatic product processing by-product contains more crustacean raw material, it is better to crush and cook in advance, which is more beneficial to the utilization of microorganism;
(3) preparation of fermentation Medium
Adjusting pH of 35% leftover, 10% brown sugar, 0.1% potassium dihydrogen phosphate, 0.05% magnesium sulfate, 0.1% ammonium sulfate, and water for 7.0 with NaOH, and sterilizing for 20-35min to obtain fermentation culture medium; the introduction of mixed bacteria is reduced as much as possible, and the influence of chlorine in the industrial water on the growth of microorganisms is reduced;
(4) fermentation production: adopting a 5000L fermentation tank for production or adopting a 15000L fermentation tank for production;
① is produced by 5000L fermentation tank by introducing fermentation medium into 5000L fermentation tank with a loading of 70-80%, inoculating the second-stage seed culture solution prepared in step (1) into the fermentation tank with an inoculation amount of 10-20%, controlling the temperature at 32 + -1 deg.C, stirring at 100-8Stopping fermentation after the product index reaches the standard per mL;
② is produced by adopting a 15000L fermentation tank, introducing the fermentation medium into the 15000L fermentation tank with a loading of 70% -80%, inoculating the second-stage seed culture solution prepared in step (1) into the fermentation tank with an inoculation amount of 10-20%, controlling the temperature at 32 + -1 deg.C, stirring at 100-8Stopping fermentation after the product index reaches the standard per mL;
(5) fermentation liquor blending and bottling:
blending the fermentation liquor to obtain a micro-ecological nutrient solution; the quality of the micro-ecological nutrient solution meets the requirement of people's republic of ChinaCompound microbial fertilizer (NY/T798-2015) according to national agricultural industry standard, and viable count>108The content of amino acid is more than or equal to 10.0g/L, the content of organic matters is more than or equal to 150g/L, and the content of Ca is more than or equal to 5.0 g/L.
Further, the formula of the primary seed culture medium in the step (1) is as follows: 3g/L beef extract, 10g/L peptone, 5g/L sodium chloride, pH7.0-7.2, sterilizing at 121 deg.C for 20 min.
Further, the formula of the secondary seed culture medium in the step (1) is as follows: 100g/L of brown sugar, 5g/L of dipotassium phosphate, 5g/L of ammonium sulfate, 1g/L of magnesium sulfate, 7.0-7.2 of pH value and 20min of sterilization at 110 ℃.
Further, the quality requirements of the qualified first-stage seed culture solution and the qualified second-stage seed culture solution in the step (1) are as follows: pH of 4.0-4.5, viable count > 109No peculiar smell, light sweet taste and wine fragrance.
According to the technical scheme, compared with the prior art, the invention discloses a method for producing the microecological nutrient solution by using the aquatic product processing byproducts, the microecological nutrient solution contains the microecological flora of microorganisms, mainly comprises lactic acid bacteria, saccharomycetes, bacillus and the like, and the viable count reaches 108More than one/mL; also contains abundant nutrient components, mainly including protein peptide, small molecular amino acid, fatty acid, vitamin and organic calcium; has strong microorganism ecological regulation function and can provide rich low molecular weight active substances. The micro-ecological nutrient solution can promote the growth of cultured aquatic organisms, purify water quality, improve the culture water environment and reduce diseases; the fertilizer can also be applied to planting of tea trees and famous and high-quality fruit trees, improve soil environment through root irrigation, provide nutrients, improve growth speed and increase yield; after the foliar spraying, the accumulation of secondary metabolites of the tea can be effectively improved, the quality of the tea is improved, and the economic benefit is improved; can be directly used as the nutrient of the foliar fertilizer for crops, so that the color of fruits or leaves of fruits, vegetables and the like is lightened, and the yield and the quality are improved.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.
FIG. 1 is a flow chart of the method for producing the micro-ecological nutrient solution by using aquatic product processing byproducts according to the invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
Treatment of pre-production fermentation equipment: checking the air tightness of the fermentation tank; cleaning a fermentation tank system by using hot water, and opening a stirrer; the boiler equipment provides steam to sterilize the whole fermentation tank system, and the action time of the steam is at least 1 h; ensuring no mixed bacteria pollution in the subsequent fermentation production.
A method for producing a micro-ecological nutrient solution by using aquatic product processing byproducts comprises the following specific steps:
(1) preparation of seed liquid
Preparing a seed suspension: and (3) performing amplification culture on the activated spore bacteria, saccharomycetes and lactic acid bacteria according to the ratio of 2: 1: 3, mixing the raw materials in proportion to prepare seed suspension;
seed expanding culture: inoculating the prepared seed suspension into a 100L primary seed tank containing a primary seed culture medium at an inoculum size of 10%, loading volume of 65-75%, stirring at 30-35 deg.C and rotation speed of 80-100r/min, ventilating 1L/min, and tank pressure of 0.10-0.13Mpa, and culturing for 36 h; taking fermentation liquor to detect pH and viable count every 6h to obtain qualified first-grade seed culture solution; continuing expanding culture: inoculating the primary seed culture solution into a 500L secondary seed tank containing a secondary seed culture medium at an inoculum size of 10%, loading volume of 65-75%, stirring at 30-35 deg.C and rotation speed of 90-120r/min, ventilating 1L/min, and tank pressure of 0.10-0.13Mpa, and continuously culturing for 36 h; taking fermentation liquor to detect pH and viable count every 6h to obtain qualified secondary seed culture solution;
(2) pretreatment of raw materials
Cooking the aquatic product processing by-product according to a solid-to-liquid ratio of 2:1, boiling for 30-45min to obtain leftovers;
(3) preparation of fermentation Medium
Adjusting pH of 35% leftover, 10% brown sugar, 0.1% potassium dihydrogen phosphate, 0.05% magnesium sulfate, 0.1% ammonium sulfate, and water for 7.0 with NaOH, and sterilizing for 20-35min to obtain fermentation culture medium;
(4) production is carried out by adopting a 5000L fermentation tank: introducing the fermentation medium into a 5000L fermentation tank with the loading of 70%; inoculating the second-stage seed culture solution prepared in the step (1) into a fermentation tank according to the inoculation amount of 20%, controlling the temperature to be 32 +/-1 ℃, stirring at the speed of 100-120r/min, ventilating at the speed of 20L/min, and actually keeping the tank pressure at 0.11-0.12 Mpa; detecting the pH value, the residual sugar concentration (%), and the viable count of the fermentation liquor every 24 hours; continuously fermenting for 5 days until pH of the fermentation liquid is 4-5.5, residual sugar content is less than 2%, and viable count exceeds 108Stopping fermentation when the strain is per mL;
(5) fermentation liquor blending and bottling:
blending the fermentation liquor to obtain a micro-ecological nutrient solution; the quality of the microecological nutrient solution meets the agricultural industry standard of the people's republic of China, namely Compound microbial fertilizer (NY/T798-2015), and the number of viable bacteria>108The content of amino acid is more than or equal to 10.0g/L, the content of organic matters is more than or equal to 150g/L, and the content of Ca is more than or equal to 5.0 g/L.
The flow chart is shown in figure 1.
Amino acid is more than or equal to 100g/L, Cu + Fe + Mn + Zn is more than or equal to 20g/L, water aqua
Test example 1 fertilizer efficiency test of micro-ecological nutrient solution on tea leaves
Firstly, a test specimen: tea of Tieguanyin variety
A soil fertilizer technology popularization station in Huaan county and a soil fertilizer technology station in Zhangzhou are entrusted by Hengxiang fishery limited company in Fujian province, and a fertilizer efficiency test of the micro-ecological nutrient solution (the micro-ecological nutrient solution prepared in example 1) is carried out in Hua-an county Huafeng town Huafeng village according to the requirements of general fertilizer effect test and evaluation requirements (NY/T2544-. The purpose of the test is to verify the fertilizer efficiency of the micro-ecological nutrient solution produced by Hengxiang fishery limited company in Fujian province on the tea, namely the influence of the tested fertilizer on the growth condition, yield and output value of the tea.
(1) Test soil: the soil of the test field is a yellow mud field, the organic matter of the soil is 18.1g/kg, the total nitrogen is 0.98g/kg, the alkaline hydrolysis nitrogen is 65.2mg/kg, the available phosphorus is 19.6mg/kg, the quick-acting potassium is 74.5mg/kg, and the pH value is 4.3.
(2) Design of experiments
The experiment was set to 3 treatments:
treatment 1: spraying a micro-ecological nutrient solution and applying fertilizer conventionally;
and (3) treatment 2: spraying equivalent clear water and applying fertilizer conventionally;
and (3) treatment: and (5) applying fertilizers conventionally.
The test was repeated 3 times, and was arranged in random blocks with a cell area of 30 square meters.
(3) Fertilizing and managing
The spraying method of the micro-ecological nutrient solution on the tea (autumn tea) comprises the following steps: after the summer-heat tea is harvested, the autumn tea buds are respectively used for 1 time before germination, initial germination and growth period, namely, the first spraying is started in 9-15 months, the second spraying is started in 22 months in 9 months, the third spraying is started in 29 months in 9 months, and the spraying is totally carried out for 3 times. Using 800 times of dilution solution, each 666.7m2Spraying 200ml microecological nutrient solution mixed with 160kg water uniformly, and converting into spraying 9.0ml microecological nutrient solution mixed with 7.2kg water uniformly in every plot (30 square meters), and spraying every 666.7m2The total dosage is 600ml (total amount of 3 times of spraying). And (4) spraying the same amount of clear water in the same period of the treatment 2.
Fertilizing method (namely conventional fertilizing): 666.7m each day in 20 days of 9 months2Tea formula fertilizer (N: P) for applying potassium sulfate2O5∶K2O is 21-6-13)60kg, and applying potassium sulfate tea formula fertilizer (N: P) in each plot (30 square meters)2O5∶K2O is 21-6-13) and 2.7 kg.
The experimental autumn tea starts to be sprayed with the micro-ecological nutrient solution in 2016, 9 and 15 days, is harvested in 2016, 10 and 15 days, and the yield is actually measured. In the test, the field management measures are consistent except for different fertilization.
(4) Analysis of results
Influence of different treatments on growth condition of tea
The field survey shows that after the tea leaves are sprayed with the micro-ecological nutrient solution in the treatment 1, the plant leaves grow faster than those in the treatment 2 and the treatment 3, and the leaf color is dark green; the germination time of the bud heads in the treatment 1 is about 3 to 5 days earlier than that of the bud heads in the treatment 2 and the treatment 3. The weight of the different treated tea plant buds is shown in table 1.
TABLE 1 different treatment tea Tree weight of hundred buds (1 bud 3 leaves)
Figure BDA0002579676700000071
The results in table 1 show that the average weight of the tea buds in the treatment 1 is 96.5g, 5.93% higher than that in the treatment 2 and 6.87% higher than that in the treatment 3, which indicates that the tea shoots can be obviously increased by spraying the micro-ecological nutrient solution on the tea.
② influence of different treatments on tea (fresh tea) yield
The yield of the tea leaves (fresh tea) treated differently is shown in table 2.
TABLE 2 different treatment of tea (fresh tea) yields
Figure BDA0002579676700000072
Treatment A averages 666.7m each2The yield was 282.2kg, which was averaged over treatment 2 per 666.7m2The yield of 262.2kg is increased by 20.0kg, and the yield is increased by 7.63%; average of 666.7m per treatment 32The yield of 257.8kg is increased by 24.4kg, and the yield is increased by 9.46%.
For analysis of variance of the yield of the experimental treatment cell, F is 11.85 & gtF0.0110.92, indicating that the yield difference between treatments reached a very significant level.
The results in table 2 show that spraying of the micro-ecological nutrient solution on the conventional fertilization treatment can significantly improve the tea yield, which is probably that the amino acids and the trace element nutrients of Fe, Cu, Zn, B and Mo in the tested fertilizer are effectively absorbed by the tea leaves, thereby promoting the tea growth; the yield difference between the equal amount of clear water spraying and the conventional fertilization treatment is not obvious, which shows that the tea yield cannot be obviously improved by spraying clear water on the conventional fertilization treatment.
③ output and ratio of fresh tea leaves in different treatment rooms
The economic benefit and the yield-to-input ratio of the different treated tea (fresh tea) are shown in table 3.
TABLE 3 economic benefit to production to input ratio of different treated tea leaves (fresh tea)
Figure BDA0002579676700000081
The different treatment output values are calculated according to the price of tea (fresh tea) of 16 yuan/kg and the microecological nutrient solution of 8.0 yuan/L. Treatment 1 averages each 666.7m2The yield is increased by 20kg compared with treatment 2, the yield value is increased by 320 yuan, and the fertilizer cost is deducted by 4.8 yuan (treatment 1 is 666.7m per treatment)2The application of 600ml of microecological nutrient solution needs 4.8 yuan), and the dosage is 666.7m2Increasing income by 315.2 yuan; treatment 1 averages each 666.7m2The yield is increased by 24.4kg compared with treatment 3, the yield value is increased by 390.4 yuan, and the fertilizer cost is deducted by 4.8 yuan (treatment 1 is 666.7m per treatment)2The application of 600ml of the micro-ecological nutrient solution needs 4.8 yuan) and the spraying of the micro-ecological nutrient solution needs 60 yuan (every 666.7 m)2Applying 1 time 20 yuan, spraying 3 times 60 yuan in total), every 666.7m2Increase income 325.6 yuan.
Treatment 1 vs. treatment 2 at 666.7m intervals2Adding 4.8 yuan of micro-ecological nutrient solution, increasing the output value by 320 yuan, and the output-input ratio is 66.7: 1; treatment 1 vs. treatment 3 at 666.7m intervals2The total amount of the micro-ecological nutrient solution and the industrial money is 64.8 yuan, the output value is increased by 390.4 yuan, and the output-input ratio is 6.0: 1.
The results show that the application of the micro-ecological nutrient solution on tea (Tieguanyin) can increase both production and income.
II, test work: tea leaf of Minbei narcissus
The soil and fertilizer station and the seed station of the Ouqu city are entrusted by Hengxiang fishery limited company of Fujian province, and the fertilizer effect test of the micro-ecological nutrient solution (the micro-ecological nutrient solution prepared in example 1) on the tea leaves is carried out in the village under the small bridge town of the Ouqu city according to the requirements of fertilizer effect test and general evaluation requirements (NY/T2544-. The purpose of the test is to verify the fertilizer efficiency of the micro-ecological nutrient solution produced by Hengxiang fishery limited company in Fujian province on the tea, namely the influence of the tested fertilizer on the growth condition, yield and output value of the tea.
(1) Test soil: the soil type of the test field is mountain red soil, 18.0g/kg of organic matters of a plough layer, 73.00mg/kg of alkaline hydrolysis nitrogen, 9.5mg/kg of quick-acting phosphorus, 77mg/kg of quick-acting potassium, 5.5 of pH value and uniform soil fertility.
(2) Design of experiments
The experiment was carried out with 3 treatments, 3 replicates and a test cell area of 26m2The length is 20.0m and the width is 1.3 m.
Treatment 1: conventional fertilization and spraying of a micro-ecological nutrient solution;
and (3) treatment 2: conventional fertilization and clear water spraying contrast;
and (3) treatment: and (5) applying fertilizers conventionally.
(3) Fertilizing and managing
The spraying method of the micro-ecological nutrient solution on the tea (autumn tea) comprises the following steps: after harvesting summer tea, before germination, initial germination and growth period of autumn tea buds are respectively used for 1 time, namely, spraying is carried out for the first time in 10 months in 9 months, spraying is carried out for the second time in 20 months in 9 months, spraying is carried out for the third time in 30 months in 9 months, and spraying is carried out for 3 times totally. Using 800 times of dilution solution, each 666.7m2Adding 200ml of micro-ecological nutrient solution into 150kg of water, and uniformly spraying, namely adding 7.8ml of product into 5.8kg of water into each plot (26 square meters), wherein each volume is 666.7m2The total amount is 600 ml. And (4) spraying the same amount of clear water in the same period of the treatment 2.
Fertilizing method (namely conventional fertilizing): 666.7m each day in 20 days of 9 months2Tea formula fertilizer (N: P) for applying potassium sulfate2O5∶K2O is 21-6-13) and 50kg, and applying potassium sulfate tea formula fertilizer (N: P) in each plot (26 square meters)2O5∶K2O is 21-6-13) and 1.9 kg.
The experimental autumn tea starts to be sprayed with the micro-ecological nutrient solution in 2016, 9 and 10 days, is harvested in 2016, 10 and 15 days, and the yield is actually measured. In the test, the field management measures are consistent except for different fertilization.
(4) Analysis of results
Influence of micro-ecological nutrient solution on growth condition of tea
Through field observation and record, from the growth vigor of the tea, the leaf color of the treated tea 1 is obviously larger and thicker than the area of the treated tea 2 and the treated tea 3, the leaf color is dark green, and the plants are stronger. The results of field observations of the growth of the stem and leaf are shown in Table 4.
TABLE 4 field observations of tea growth
Figure BDA0002579676700000091
The average stem and leaf length of treatment 1 was 17.7cm, the average stem and leaf length of treatment 2 was 16.2cm, and the average stem and leaf length of treatment 3 was 14.9 cm. The average length of stem and leaf is increased by 1.5cm in the treatment 1 compared with the treatment 2, and the average length is increased by 2.8cm in the treatment 3. Average leaf area of 32.8cm for treatment 12Treatment 2 average leaf area 30.0cm2Treatment 3 average leaf area 28.2cm2The average leaf area of the treatment 1 is increased by 2.8cm compared with that of the treatment 22Increase by 4.6cm compared with treatment 32. Compared with the blank control of spraying clear water, the method for spraying the micro-ecological nutrient solution on the leaf surface can effectively increase the length of the stem leaves of the tea leaves and the area of the tea leaves.
② influence of micro-ecological nutrient solution on fresh tea yield
The tea yield for the different treatments is shown in table 5.
TABLE 5 different treatment of tea yield
Figure BDA0002579676700000101
Table 5 shows that by measuring the yield, the average of the treated fresh tea is 666.7m per fresh tea 12The yield is 694.9kg, and the average of fresh tea processed by the method 2 is 666.7m2The yield is 607.7kg, and the average of processed 3 fresh tea is 666.7m2The yield was 579.5 kg. That is, compared with the blank control treatment 2 of spraying clear water and the conventional fertilization treatment 3, the fresh tea has the average length of 666.7m for the treatment 1 of spraying the micro-ecological nutrient solution on the leaf surfaces2The yield is increased by 87.2kg and 115.4 kg; the amplification rates are 14.3% and 19.9%, respectively. Therefore, compared with the control, the micro-ecological nutrient solution for spraying on the tea has better effect. The results of the test were analyzed for variance and multiple comparisons, F ═ 14.42 > F0.05At 6.94, the inter-treatment differences reached a significant level. The above results show that: the micro-ecological nutrient solution is sprayed on the conventional fertilization treatment, so that the tea yield can be obviously improved, and the amino acid and the trace element nutrients of Fe, Cu, Zn, B and Mo in the fertilizer to be tested can be effectively absorbed by the tea leaves, so that the tea growth is promoted; the yield difference between the equal amount of clear water spraying and the conventional fertilization treatment is not obvious, which shows that the tea yield cannot be obviously improved by spraying clear water on the conventional fertilization treatment.
Impact of spraying micro-ecological nutrient solution on economic benefit of tea
The economic benefit to production to yield ratio for the different treatments are shown in table 6.
TABLE 6 economic benefit to production to input ratio for different treatments
Figure BDA0002579676700000111
The price of the fresh tea leaves is as follows: 6.0 yuan/kg and 8.0 yuan/L of the micro-ecological nutrient solution. Treatment 1 averages each 666.7m2The yield is increased by 87.2 kg/666.6 m on average compared with the yield increased by 2 spray water2Increasing yield 523.2 yuan/666.6 m2Deducting 4.8 yuan of fertilizer cost (treating 1 per 666.7 m)2The application of 600ml of microecological nutrient solution needs 4.8 yuan), and the dosage is 666.7m2Increase income 518.4 yuan; treatment 1 averages each 666.7m2The yield is increased by 115.4 kg/666.6 m on average compared with that of the conventional fertilization treated by 32Increasing yield 692.4 yuan/666.6 m2Deducting 4.8 yuan of fertilizer cost (treating 1 per 666.7 m)2The application of 600ml of the micro-ecological nutrient solution needs 4.8 yuan) and the spraying of the micro-ecological nutrient solution needs 60 yuan (every 666.7 m)2Applying 1 time 20 yuan, spraying 3 times 60 yuan in total), every 666.7m2Increase yield to 627.6 yuan.
Treatment 1 vs. treatment 2 at 666.7m intervals2Adding 4.8 yuan of micro-ecological nutrient solution, increasing the yield value to 523.2 yuan, wherein the yield-to-feed ratio is 109: 1; treatment 1 vs. treatment 3 at 666.7m intervals2The total amount of the added micro-ecological nutrient solution and the industrial money is 64.8 yuan, the output value is increased by 692.4 yuan, the output-to-input ratio is 10.7: 1, and the method has good economic benefit and high output-to-input ratio.
Test example 2 fertilizer efficiency test of micro-ecological nutrient solution on pakchoi
Firstly, a test specimen: chinese cabbage of Degao Xiaohuang Bai
A soil fertilizer technology popularization station in Huaan county and a soil fertilizer technology station in Zhangzhou are entrusted by Hengxiang fishery limited company in Fujian province, and fertilizer efficiency tests containing the micro-ecological nutrient solution (the micro-ecological nutrient solution prepared in example 1) are developed in Hua-an county in Huafeng town of Huafeng according to the requirements of general fertilizer effect tests and evaluation requirements (NY/T2544-. The purpose of the test is to verify the fertilizer efficiency of the micro-ecological nutrient solution produced by Hengxiang fishery limited company in Fujian province on the pakchoi, namely the influence of the tested fertilizer on the growth condition, yield and output value of the pakchoi.
(1) Test soil: the soil of the test field is retention type rice soil (lime mud field), the organic matter of the soil is 23.1g/kg, the total nitrogen is 1.08g/kg, the alkaline hydrolysis nitrogen is 76.4mg/kg, the available phosphorus is 29.8mg/kg, the quick-acting potassium is 81.3mg/kg, and the pH value is 5.7.
(2) Design of experiments
The experiment was set to 3 treatments:
treatment 1: spraying a micro-ecological nutrient solution and applying fertilizer conventionally;
and (3) treatment 2: spraying equivalent clear water and applying fertilizer conventionally;
and (3) treatment: and (5) applying fertilizers conventionally.
The test was repeated 3 times, and was arranged in random blocks with a cell area of 30 square meters.
(3) Fertilizing and managing
The method for spraying the micro-ecological nutrient solution on the pakchoi comprises the following steps: the treatment 1 is carried out by spraying for the first time at 10 days after seedling transplantation, namely 10 months and 30 days, spraying for the second time at 11 months and 10 days, and spraying for 2 times in total. Using 800 times of dilution solution, each 666.7m2Spraying 200ml of product mixed with 160kg of water uniformly, and converting into spraying with 9.0ml of product mixed with 7.2kg of water uniformly in each district (30 square meters), wherein each spraying is carried out at a rate of 666.7m2The total dosage is 400 ml. And (4) spraying the same amount of clear water in the same period of the treatment 2.
Fertilizing method (namely conventional fertilizing): applying 1.35 kg and 2.25 kg of domestic compound fertilizer (23:7:15) respectively in 10 months and 25 days and 11 months and 10 days (the total amount of the two times is 666.7m each)280 kg applied).
The test is transplanted in 2016, 10 months and 20 days, and is harvested in 2016, 11 months and 25 days, and the yield is actually measured. In the test, the field management measures are consistent except for different fertilization.
(4) Analysis of results
Influence of different treatments on growth conditions of pakchoi
The field survey shows that the stem and leaf growth of the pakchoi can be promoted after the treatment 1 is sprayed with the micro-ecological nutrient solution, and the leaf color is dark green. The measurement of the pakchoi when being harvested in 11 months and 25 days shows that the leaf of the pakchoi treated by 1 is wider than that of the pakchoi treated by 2 and 3, the average plant height of the pakchoi treated by 1 is 33.1cm, and the average plant height of the pakchoi treated by 2 and 3 is respectively increased by 1.9cm and 2.3 cm.
② influence of different treatments on yield of pakchoi
The yield of the cabbages treated differently is shown in table 7.
TABLE 7 yield of Chinese cabbage treated differently
Figure BDA0002579676700000121
Treatment 1 averages each 666.7m2The yield was 1807kg, which was averaged over treatment 2 per 666.7m2The yield of 1665kg is increased by 142kg, and the yield is increased by 8.53%; average of 666.7m per treatment 32The yield of 1660kg is increased by 147kg, and the yield is increased by 8.86%; treatment 2 averaged 666.7m more than treatment 32The yield is increased by 5kg and increased by 0.30 percent.
For analysis of variance of the yield of the experimental treatment cell, F is 8.16 & gtF0.05＝5.14，F＜F0.0110.92, indicating that the yield difference between treatments is significant.
Table 7 the results show that: the yield of the pakchoi can be obviously improved by spraying the micro-ecological nutrient solution on the conventional fertilization treatment, which is probably that the amino acid and the trace element nutrients of Fe, Cu, Zn, B and Mo in the fertilizer to be tested are effectively absorbed by the leaves of the pakchoi, so that the growth of the pakchoi is promoted; the yield difference between the equivalent amount of clear water sprayed and the conventional fertilization treatment is not obvious, which shows that the yield of the pakchoi cannot be obviously improved by spraying the clear water on the conventional fertilization treatment.
③ the yield and the yield-to-investment ratio of the pakchoi in different processing rooms
The economic benefit and yield-to-input ratio of the cabbages treated differently are shown in table 8.
TABLE 8 economic benefit to production to input ratio for different treatments of pakchoi
Figure BDA0002579676700000131
The different processing output values are calculated according to the selling price of the pakchoi of 6.0 yuan/kg and the microecological nutrient solution of 8.0 yuan/L. Treatment 1 averages each 666.7m2The yield of the pakchoi is increased by 142kg compared with the yield of the pakchoi treated by 2, the yield value is increased by 852 yuan, and the increased fertilizer cost is deducted by 3.2 yuan (the treatment cost is 1 per 666.7 m)2The dosage of the micro-ecological nutrient solution is 3.2 yuan per 666.7m2Increasing yield 848.8 yuan; treatment 1 averages each 666.7m2The yield of the pakchoi is increased by 147kg compared with the yield of the pakchoi treated by 3, the yield is increased by 882 Yuan, and the increased fertilizer cost is deducted by 3.2 Yuan (the treatment cost is 1 per 666.7 m)2The amount of the micro-ecological nutrient solution is 3.2 yuan for applying 400ml of the micro-ecological nutrient solution) and 40 yuan for spraying the micro-ecological nutrient solution (every 666.7 m)21 application of 20 yuan), each 666.7m2The yield is 838.8 yuan.
Treatment 1 vs. treatment 2 at 666.7m intervals2Adding 3.2 yuan of micro-ecological nutrient solution, increasing the output value to 852 yuan, and increasing the output-to-input ratio to 266: 1; treatment 1 vs. treatment 3 at 666.7m intervals2The total amount of the micro-ecological nutrient solution and the industrial money is 43.2 yuan, the output value is increased by 882 yuan, and the output-input ratio is 20.4: 1.
The results show that the yield and income can be increased by applying the micro-ecological nutrient solution on the Chinese cabbage (Degaohuahuang).
II, test work: chinese cabbage of the species ilex purpurea
The soil fertilizer station and the house way agricultural technology station of the Jianzu city are entrusted by Hengxiang fishery limited company of Fujian province, and the fertilizer effect test of the micro-ecological nutrient solution (the micro-ecological nutrient solution prepared in the example 1) is carried out in seven villages of the house way and town of the Jianzu city according to the requirements of general fertilizer effect test and evaluation requirements (NY/T2544-. The purpose of the test is to verify the fertilizer efficiency of the micro-ecological nutrient solution produced by Hengxiang fishery limited company in Fujian province on the pakchoi, namely the influence of the tested fertilizer on the growth condition, yield and output value of the pakchoi.
(1) Test soil: the soil type is a plaster field, the organic matter of a plough layer is 30.0g/kg, the alkaline hydrolysis nitrogen is 129mg/kg, the quick-acting phosphorus is 17.5mg/kg, the quick-acting potassium is 112mg/kg, the pH value is 5.8, and the soil fertility is uniform.
(2) Design of experiments
The experiment was carried out with 3 treatments, 3 replicates, a test cell area of 21m2。
Treatment 1: conventional fertilization and foliar spraying of a micro-ecological nutrient solution;
and (3) treatment 2: conventional fertilization and clear water spraying contrast;
and (3) treatment: and (5) applying fertilizers conventionally.
(3) Fertilizing and managing
Uniformly spraying 800 times of diluent of the microecological nutrient solution on the leaf surface 7 days after the Chinese cabbage is sown, wherein the thickness of the diluent is 666.7m2The dosage is 200ml each time, the water is added by 120kg, the amount is converted into that 6.3ml of micro-ecological nutrient solution is uniformly sprayed by adding 3.8kg of water in each district (21 square meters), the spraying is carried out once every 10 days, the spraying is carried out for 2 times totally, and each time is 666.7m2The total dosage is 400 ml. And (3) spraying equal amount of clear water in the same period of the treatment 2, wherein other management methods are consistent in the growth period.
And (3) conventional fertilization: 115kg/666.7m 29% compound fertilizer2(90 kg of base fertilizer and 15kg of additional fertilizer). The base fertilizer is applied before sowing, and the additional fertilizer is applied 15 days after sowing. Sowing in 10 months and 30 days, fertilizing for the first time in 10 months and 30 days, fertilizing for the second time in 11 months and 15 days, and harvesting in 12 months and 10 days. Treating 1, spraying the micro-ecological nutrient solution for 2 times, and applying the micro-ecological nutrient solution respectively in 11 months, 10 days and 11 months, 20 days; and (4) spraying the same amount of clear water in the same period of the treatment 2. And single yield and single production are measured and calculated in the community.
(4) Analysis of results
Influence of micro-ecological nutrient solution on growth condition of pakchoi
Through field observation and record, from the growth vigor of the pakchoi, the leaf color of the treated 1 is obviously larger and thicker than the leaf areas of the treated 2 and the treated 3, the leaf color is dark green, and the plants are stronger. The average plant height of the treatment 1 is 40cm, the average plant height of the treatment 2 is 37cm, and the average plant height of the treatment 3 is 36 cm. The plant height of the treatment 1 is increased by 3cm on average compared with that of the treatment 2, and is increased by 4cm compared with that of the treatment 3. The difference between the height of the treated 2 plants and the height of the treated 3 plants is 1cm, and the difference is not obvious. Compared with the blank control of spraying clear water, the method for spraying the microecological nutrient solution on the leaf surfaces can effectively increase the plant height of the pakchoi.
② influence of micro-ecological nutrient solution on yield of pakchoi
The yield of the cabbages treated differently is shown in table 9.
TABLE 9 Chinese cabbage yield for different treatments
Figure BDA0002579676700000151
Treatment 1 averages each 666.7m2The yield was 1200.1kg, treatment 2 averaged 666.7m2The yield was 1098.5kg, treatment 3 averaged 666.7m2The yield was 1092.1 kg. Namely, compared with the blank control treatment 2 of spraying clear water and the conventional fertilization treatment 3, the treatment 1 of spraying the microecological nutrient solution on the leaf surfaces of the pakchoi has the average of 666.7m2The yield is increased by 101.6kg, the amplification is 8.47 percent and the average grain size is 666.7m2The yield is increased by 108.0kg and the amplification is increased by 9.0 percent. It can be seen that the spraying of the micro-ecological nutrient solution on the pakchoi has better effect compared with the contrast.
For analysis of variance of the yield of the experimental treatment cell, F is 86.18 & gtF0.0118.00, indicating that the yield difference between treatments reached a very significant level. Table 9 the results show that: the yield of the pakchoi can be obviously improved by spraying the micro-ecological nutrient solution on the conventional fertilization treatment, which is probably that the amino acid and the trace element nutrients of Fe, Cu, Zn, B and Mo in the fertilizer to be tested are effectively absorbed by the leaves of the pakchoi, so that the growth of the pakchoi is promoted; the yield difference between the equivalent amount of clear water sprayed and the conventional fertilization treatment is not obvious, which shows that the yield of the pakchoi cannot be obviously improved by spraying the clear water on the conventional fertilization treatment.
Influence of spraying of micro-ecological nutrient solution on economic benefit of pakchoi
The economic benefit to production to yield ratio for the different treatments are shown in table 10.
TABLE 10 economic benefit to production to input ratio for different treatments of pakchoi
Figure BDA0002579676700000152
The selling price of the pakchoi is 6.0 yuan/kg, the microecological nutrient solution is 8.0 yuan/L, and the average value is 1Each 666.7m2Compared with the yield of 2-treated pakchoi, the yield is increased by 609.6 yuan, and the increased fertilizer cost is deducted by 3.2 yuan (every 666.7m for 1-treated pakchoi)2The dosage of the micro-ecological nutrient solution is 3.2 yuan per 666.7m2Increasing yield 606.4 yuan; treatment 1 averages each 666.7m2The yield of pakchoi is increased by 648.0 yuan compared with that of treatment 3, and the increased fertilizer cost is deducted by 3.2 yuan (treatment 1 is 666.7m per unit)2The amount of the micro-ecological nutrient solution is 3.2 yuan for applying 400ml of the micro-ecological nutrient solution) and 40 yuan for spraying the micro-ecological nutrient solution (every 666.7 m)21 application of 20 yuan), each 666.7m2Increase yield to 604.8 yuan.
Treatment 1 vs. treatment 2 at 666.7m intervals23.2 yuan of micro-ecological nutrient solution is added, the output value is increased by 609.6 yuan, and the output-input ratio is 190: 1; treatment 1 vs. treatment 3 at 666.7m intervals2The total amount of amino acid-containing water-soluble fertilizer and industrial money is 43.2 yuan, the yield is increased by 648 yuan, the yield-to-input ratio is 15: 1, and the method has good economic benefit and high yield-to-input ratio.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims (4)

1. A method for producing a micro-ecological nutrient solution by using aquatic product processing byproducts is characterized by comprising the following specific steps:
(1) preparation of seed liquid
Preparing a seed suspension: and (3) performing amplification culture on the activated spore bacteria, saccharomycetes and lactic acid bacteria according to the ratio of 2: 1: 3, mixing the raw materials in proportion to prepare seed suspension;
seed expanding culture: inoculating the prepared seed suspension into a 100L primary seed tank containing a primary seed culture medium at an inoculum size of 10%, loading volume of 65-75%, stirring at 30-35 deg.C and rotation speed of 80-100r/min, ventilating 1L/min, and tank pressure of 0.10-0.13Mpa, and culturing for 36 h; taking fermentation liquor to detect pH and viable count every 6h to obtain qualified first-grade seed culture solution; continuing expanding culture: inoculating the primary seed culture solution into a 500L secondary seed tank containing a secondary seed culture medium at an inoculum size of 10%, loading volume of 65-75%, stirring at 30-35 deg.C and rotation speed of 90-120r/min, ventilating 1L/min, and tank pressure of 0.10-0.13Mpa, and continuously culturing for 36 h; taking fermentation liquor to detect pH and viable count every 6h to obtain qualified secondary seed culture solution;
(2) pretreatment of raw materials
Cooking the aquatic product processing by-product according to a solid-to-liquid ratio of 2:1, boiling for 30-45min to obtain leftovers;
(3) preparation of fermentation Medium
Adjusting pH of 35% leftover, 10% brown sugar, 0.1% potassium dihydrogen phosphate, 0.05% magnesium sulfate, 0.1% ammonium sulfate, and water for 7.0 with NaOH, and sterilizing for 20-35min to obtain fermentation culture medium;
(4) fermentation production: adopting a 5000L fermentation tank for production or adopting a 15000L fermentation tank for production;
① is produced by 5000L fermentation tank by introducing fermentation medium into 5000L fermentation tank with a loading of 70-80%, inoculating the second-stage seed culture solution prepared in step (1) into the fermentation tank with an inoculation amount of 10-20%, controlling the temperature at 32 + -1 deg.C, stirring at 100-8Stopping fermentation when the strain is per mL;
② is produced by adopting a 15000L fermentation tank, introducing the fermentation medium into the 15000L fermentation tank with a loading of 70% -80%, inoculating the second-stage seed culture solution prepared in step (1) into the fermentation tank with an inoculation amount of 10-20%, controlling the temperature at 32 + -1 deg.C, stirring at 100-8Stopping fermentation when the strain is per mL;
(5) fermentation liquor blending and bottling:
blending the fermentation liquor to obtain a micro-ecological nutrient solution; the quality of the microecological nutrient solution meets the agricultural industry standard of the people's republic of China, namely Compound microbial fertilizer (NY/T798-2015), and the number of viable bacteria>108The content of amino acid is more than or equal to 10.0g/L, the content of organic matters is more than or equal to 150g/L, and the content of Ca is more than or equal to 5.0 g/L.
2. The method for producing a micro-ecological nutrient solution by using aquatic product processing byproducts as claimed in claim 1, wherein the primary seed culture medium formula in the step (1) is as follows: 3g/L beef extract, 10g/L peptone, 5g/L sodium chloride, pH7.0-7.2, sterilizing at 121 deg.C for 20 min.
3. The method for producing a micro-ecological nutrient solution by using aquatic product processing byproducts as claimed in claim 1, wherein the secondary seed culture medium formula in step (1) is as follows: 100g/L of brown sugar, 5g/L of dipotassium phosphate, 5g/L of ammonium sulfate, 1g/L of magnesium sulfate, 7.0-7.2 of pH value and 20min of sterilization at 110 ℃.
4. The method for producing micro-ecological nutrient solution by using aquatic product processing byproducts, according to claim 1, wherein the quality requirements of the qualified primary seed culture solution and qualified secondary seed culture solution in the step (1) are as follows: pH of 4.0-4.5, viable count > 109No peculiar smell, light sweet taste and wine fragrance.
CN202010664032.7A 2020-07-10 2020-07-10 Method for producing micro-ecological nutrient solution by using aquatic product processing by-products Pending CN111758856A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202010664032.7A CN111758856A (en) 2020-07-10 2020-07-10 Method for producing micro-ecological nutrient solution by using aquatic product processing by-products

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202010664032.7A CN111758856A (en) 2020-07-10 2020-07-10 Method for producing micro-ecological nutrient solution by using aquatic product processing by-products

Publications (1)

Publication Number Publication Date
CN111758856A true CN111758856A (en) 2020-10-13

Family

ID=72724970

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202010664032.7A Pending CN111758856A (en) 2020-07-10 2020-07-10 Method for producing micro-ecological nutrient solution by using aquatic product processing by-products

Country Status (1)

Country Link
CN (1) CN111758856A (en)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102860408A (en) * 2012-09-24 2013-01-09 江西昌丰由由生物科技有限公司 Preparation method of multifunctional live microorganism preparation for forage
CN105053026A (en) * 2015-09-02 2015-11-18 福建省恒祥渔业有限公司 Method for producing plant growth regulator by utilizing aquatic products processing waste
CN105505823A (en) * 2015-12-31 2016-04-20 北京沃土天地生物科技有限公司 Compound microbial agent, water-soluble fertilizer containing same and applications of compound microbial agent and water-soluble fertilizer
CN105861615A (en) * 2016-04-22 2016-08-17 闫大凤 Production method of amino acid bioactive peptide for aquatic products
CN107312735A (en) * 2017-09-04 2017-11-03 天津市农业生物技术研究中心 One Yeasts and lactic acid bacteria mixed synchronization fermentation prepare method and the application of probiotics
CN109123076A (en) * 2018-08-30 2019-01-04 辽宁威兰生物技术有限责任公司 A kind of production method of livestock and poultry vitamin B2 auxotype probiotics

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102860408A (en) * 2012-09-24 2013-01-09 江西昌丰由由生物科技有限公司 Preparation method of multifunctional live microorganism preparation for forage
CN105053026A (en) * 2015-09-02 2015-11-18 福建省恒祥渔业有限公司 Method for producing plant growth regulator by utilizing aquatic products processing waste
CN105505823A (en) * 2015-12-31 2016-04-20 北京沃土天地生物科技有限公司 Compound microbial agent, water-soluble fertilizer containing same and applications of compound microbial agent and water-soluble fertilizer
CN105861615A (en) * 2016-04-22 2016-08-17 闫大凤 Production method of amino acid bioactive peptide for aquatic products
CN107312735A (en) * 2017-09-04 2017-11-03 天津市农业生物技术研究中心 One Yeasts and lactic acid bacteria mixed synchronization fermentation prepare method and the application of probiotics
CN109123076A (en) * 2018-08-30 2019-01-04 辽宁威兰生物技术有限责任公司 A kind of production method of livestock and poultry vitamin B2 auxotype probiotics

Similar Documents

Publication Publication Date Title
CN102010240B (en) Plant enzyme foliar fertilizer and preparation method thereof
CN101786914B (en) Microorganism fertilizer for promoting rice production, preparation method and application thereof
CN102010241A (en) Enzyme microbial organic fertilizer and preparation method thereof
CN110129215B (en) Compound microbial preparation and application thereof
CN107580818B (en) A kind of integrated approach of soil conditioning and reparation
CN105272409A (en) Cherry tomato culture medium and preparation method thereof
CN101948780B (en) Antagonist bacterium for preventing and treating continuous cropping hot pepper epidemic disease and microbial organic fertilizer thereof
CN103194410B (en) Paenibacillus mucilaginosus and method for producing compound microorganism bacterium agent by utilizing same
CN101475809A (en) Soil conditioner for lon-cultivated land of ginseng
CN105441360A (en) Organic fertilizer fermentation inoculant
CN104744182A (en) Tobacco water retention biological organic fertilizer and preparation method thereof
CN105601347A (en) Preparation method for crop organic fertilizer
CN102249752A (en) Microbial fertilizer and preparation method and application thereof
CN112205274A (en) Rice seedling raising matrix based on various agricultural wastes and preparation method thereof
CN107338056B (en) Green microbial soil conditioner and preparation method thereof
CN114702357B (en) High-activity compound microbial fertilizer and preparation method thereof
CN103351188B (en) Preparation method of agricultural organic fertilizer by treating pure straws as raw material
CN106518527A (en) Biological fertilizer prepared from tea leaves and preparation method of biological fertilizer
CN109836298A (en) A kind of biological humic acid ferment liquid fertilizer and the preparation method and application thereof
CN102276337A (en) Re-cultivation long-acting bio-organic fertilizer
CN108383653A (en) A kind of method and bio-organic fertilizer preparing fertilizer using stalk
CN109160862A (en) A kind of amino acid bio-organic fertilizer and the preparation method and application thereof
CN112655719A (en) Microbial preparation for promoting rice rooting and preparation method and application thereof
CN103964928A (en) Preparation method of microorganism soil restoration agent
CN108101707B (en) Total nutrient microbial amino acid water-soluble fertilizer and preparation method thereof

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination