CN111727261B - 非对称pcr方法 - Google Patents
非对称pcr方法 Download PDFInfo
- Publication number
- CN111727261B CN111727261B CN201880075613.8A CN201880075613A CN111727261B CN 111727261 B CN111727261 B CN 111727261B CN 201880075613 A CN201880075613 A CN 201880075613A CN 111727261 B CN111727261 B CN 111727261B
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- primer
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- pcr
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/131—Modifications characterised by incorporating a restriction site
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/151—Modifications characterised by repeat or repeated sequences, e.g. VNTR, microsatellite, concatemer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/101—Temperature
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/107—Temperature of melting, i.e. Tm
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2531/00—Reactions of nucleic acids characterised by
- C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
- C12Q2531/107—Probe or oligonucleotide ligation
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Bridges Or Land Bridges (AREA)
- Application Of Or Painting With Fluid Materials (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Materials For Medical Uses (AREA)
- Joining Of Glass To Other Materials (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US15/820,475 US10513730B2 (en) | 2017-11-22 | 2017-11-22 | Asymmetric PCR methods, primers and kits |
| US15/820,475 | 2017-11-22 | ||
| PCT/EP2018/082086 WO2019101796A1 (en) | 2017-11-22 | 2018-11-21 | Asymmetric pcr methods |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111727261A CN111727261A (zh) | 2020-09-29 |
| CN111727261B true CN111727261B (zh) | 2024-10-29 |
Family
ID=64559658
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201880075613.8A Active CN111727261B (zh) | 2017-11-22 | 2018-11-21 | 非对称pcr方法 |
Country Status (17)
| Country | Link |
|---|---|
| US (3) | US10513730B2 (https=) |
| EP (1) | EP3714065A1 (https=) |
| JP (1) | JP7334154B2 (https=) |
| KR (1) | KR20200088447A (https=) |
| CN (1) | CN111727261B (https=) |
| AU (1) | AU2018373766A1 (https=) |
| BR (1) | BR112020009782A2 (https=) |
| CA (1) | CA3082426A1 (https=) |
| IL (1) | IL274638B2 (https=) |
| MX (1) | MX2020005245A (https=) |
| MY (1) | MY200344A (https=) |
| PH (1) | PH12020550654A1 (https=) |
| RU (1) | RU2767061C2 (https=) |
| SG (1) | SG11202004284PA (https=) |
| TW (1) | TWI822711B (https=) |
| WO (1) | WO2019101796A1 (https=) |
| ZA (1) | ZA202002570B (https=) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MX2022000628A (es) | 2019-07-19 | 2022-03-11 | Safeguard Biosystems Holdings Ltd | Deteccion de secuencias genomicas utilizando combinaciones de sondas, moleculas de sonda y arrays que comprenden las sondas para la deteccion especifica de organismos. |
| CN113046420B (zh) * | 2019-12-26 | 2022-10-04 | 厦门大学 | 一种不对称扩增多个靶核酸的方法 |
| CN113046421B (zh) * | 2019-12-26 | 2022-09-30 | 厦门大学 | 一种不对称扩增靶核酸的方法 |
| JP2024504966A (ja) | 2021-01-20 | 2024-02-02 | セイフガード バイオシステムズ ホールディングズ リミテッド | 改善されたゲノム配列検出およびそのためのプローブ分子 |
| CN116745434A (zh) | 2021-01-20 | 2023-09-12 | 保障生物系统控股有限公司 | 基因组序列的改进的检测及其探针分子 |
| CN115747328A (zh) * | 2022-08-19 | 2023-03-07 | 福建师范大学 | 一种提高单链dna产量和改善产物比例的方法 |
| CN121464225A (zh) * | 2023-06-08 | 2026-02-03 | 威廉马歇莱思大学 | 被配置用于具有高动态范围的含核酸样品的高通量探询的方法和试剂盒 |
| CN120138110B (zh) * | 2025-03-14 | 2026-04-07 | 湖南大地同年生物科技有限公司 | 多重pcr检测反应中代替高温变性产生单链的方法和提高多重pcr产物液相杂交检测灵敏性和准确性的方法及配套的试剂盒 |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1629305A (zh) * | 2004-08-26 | 2005-06-22 | 北京博奥生物芯片有限责任公司 | 一种不对称pcr扩增方法及其专用引物与应用 |
| CN104293794B (zh) * | 2014-09-24 | 2017-04-12 | 南方医科大学 | 一种与β‑淀粉样前体蛋白裂解酶1特异性结合的核酸适配子及其应用 |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5066584A (en) | 1988-09-23 | 1991-11-19 | Cetus Corporation | Methods for generating single stranded dna by the polymerase chain reaction |
| US7435541B2 (en) * | 2000-05-23 | 2008-10-14 | Sequenom, Inc. | Restriction enzyme genotyping |
| US6887664B2 (en) * | 2000-06-06 | 2005-05-03 | Applera Corporation | Asynchronous primed PCR |
| AUPS076802A0 (en) * | 2002-02-26 | 2002-03-21 | Commonwealth Scientific And Industrial Research Organisation | Selective nucleic acid amplification |
| WO2004068112A2 (en) * | 2003-01-28 | 2004-08-12 | Gorilla Genomics, Inc. | Hairpin primer amplification |
| JP4322554B2 (ja) * | 2003-05-19 | 2009-09-02 | 株式会社ニチレイフーズ | Ｄｎａ増幅反応の効率向上方法 |
| CN100494399C (zh) * | 2003-06-30 | 2009-06-03 | 清华大学 | 一种基于dna芯片的基因分型方法及其应用 |
| ES2665690T3 (es) | 2007-02-02 | 2018-04-26 | Genera Biosystems Limited | Generación de moléculas de ácido nucleico |
| CN103459612A (zh) * | 2010-12-03 | 2013-12-18 | 布兰代斯大学 | 用于检测野生型群体中的核酸突变体的方法和试剂盒 |
| WO2014052487A1 (en) * | 2012-09-28 | 2014-04-03 | Cepheid | Two-primer pcr for microrna multiplex assay |
| WO2014160059A1 (en) * | 2013-03-13 | 2014-10-02 | Gen9, Inc. | Compositions and methods for synthesis of high fidelity oligonucleotides |
| NO337188B1 (no) * | 2013-04-08 | 2016-02-08 | Niva | COMPAS-PCR fremgangsmåte og fremgangsmåter for påvisning, identifisering eller overvåking av laksearter, kit, samt anvendelse av metoden og kit |
| CA2911303C (en) * | 2013-05-07 | 2021-02-16 | Micronics, Inc. | Methods for preparation of nucleic acid-containing samples using clay minerals and alkaline solutions |
| EP3181700A1 (en) | 2015-12-18 | 2017-06-21 | Safeguard Biosystems Holdings Ltd. | Three-dimensional polymer networks with channels situated therein |
| EP3199629A1 (en) | 2016-01-30 | 2017-08-02 | Safeguard Biosystems Holdings Ltd. | Bead beating tube and method for extracting deoxyribonucleic acid and/or ribonucleic acid from microorganisms |
-
2017
- 2017-11-22 US US15/820,475 patent/US10513730B2/en active Active
-
2018
- 2018-11-21 KR KR1020207018029A patent/KR20200088447A/ko active Pending
- 2018-11-21 BR BR112020009782-9A patent/BR112020009782A2/pt not_active Application Discontinuation
- 2018-11-21 US US16/765,601 patent/US20200308629A1/en not_active Abandoned
- 2018-11-21 WO PCT/EP2018/082086 patent/WO2019101796A1/en not_active Ceased
- 2018-11-21 MX MX2020005245A patent/MX2020005245A/es unknown
- 2018-11-21 JP JP2020528180A patent/JP7334154B2/ja active Active
- 2018-11-21 CA CA3082426A patent/CA3082426A1/en active Pending
- 2018-11-21 IL IL274638A patent/IL274638B2/en unknown
- 2018-11-21 AU AU2018373766A patent/AU2018373766A1/en not_active Abandoned
- 2018-11-21 SG SG11202004284PA patent/SG11202004284PA/en unknown
- 2018-11-21 MY MYPI2020002329A patent/MY200344A/en unknown
- 2018-11-21 EP EP18811474.8A patent/EP3714065A1/en active Pending
- 2018-11-21 CN CN201880075613.8A patent/CN111727261B/zh active Active
- 2018-11-21 RU RU2020119664A patent/RU2767061C2/ru active
- 2018-11-22 TW TW107141708A patent/TWI822711B/zh active
-
2020
- 2020-05-08 ZA ZA2020/02570A patent/ZA202002570B/en unknown
- 2020-05-18 PH PH12020550654A patent/PH12020550654A1/en unknown
-
2025
- 2025-04-07 US US19/171,538 patent/US20250290125A1/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1629305A (zh) * | 2004-08-26 | 2005-06-22 | 北京博奥生物芯片有限责任公司 | 一种不对称pcr扩增方法及其专用引物与应用 |
| CN104293794B (zh) * | 2014-09-24 | 2017-04-12 | 南方医科大学 | 一种与β‑淀粉样前体蛋白裂解酶1特异性结合的核酸适配子及其应用 |
Also Published As
| Publication number | Publication date |
|---|---|
| CA3082426A1 (en) | 2019-05-31 |
| US20200308629A1 (en) | 2020-10-01 |
| US20250290125A1 (en) | 2025-09-18 |
| US20190153508A1 (en) | 2019-05-23 |
| RU2020119664A (ru) | 2021-12-23 |
| AU2018373766A1 (en) | 2020-05-28 |
| IL274638B2 (en) | 2025-03-01 |
| IL274638A (en) | 2020-06-30 |
| CN111727261A (zh) | 2020-09-29 |
| IL274638B1 (en) | 2024-11-01 |
| KR20200088447A (ko) | 2020-07-22 |
| BR112020009782A2 (pt) | 2020-10-13 |
| TWI822711B (zh) | 2023-11-21 |
| JP2021503903A (ja) | 2021-02-15 |
| EP3714065A1 (en) | 2020-09-30 |
| MX2020005245A (es) | 2020-08-24 |
| RU2020119664A3 (https=) | 2021-12-23 |
| RU2767061C2 (ru) | 2022-03-16 |
| MY200344A (en) | 2023-12-20 |
| SG11202004284PA (en) | 2020-06-29 |
| US10513730B2 (en) | 2019-12-24 |
| TW201925480A (zh) | 2019-07-01 |
| PH12020550654A1 (en) | 2021-04-19 |
| ZA202002570B (en) | 2021-08-25 |
| JP7334154B2 (ja) | 2023-08-28 |
| WO2019101796A1 (en) | 2019-05-31 |
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| GR01 | Patent grant |