RU2020119664A3 - - Google Patents

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Publication number
RU2020119664A3
RU2020119664A3 RU2020119664A RU2020119664A RU2020119664A3 RU 2020119664 A3 RU2020119664 A3 RU 2020119664A3 RU 2020119664 A RU2020119664 A RU 2020119664A RU 2020119664 A RU2020119664 A RU 2020119664A RU 2020119664 A3 RU2020119664 A3 RU 2020119664A3
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RU
Russia
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RU2020119664A
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Russian (ru)
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RU2020119664A (ru
RU2767061C2 (ru
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6848Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10Modifications characterised by
    • C12Q2525/131Modifications characterised by incorporating a restriction site
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10Modifications characterised by
    • C12Q2525/151Modifications characterised by repeat or repeated sequences, e.g. VNTR, microsatellite, concatemer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00Reactions demanding special reaction conditions
    • C12Q2527/101Temperature
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00Reactions demanding special reaction conditions
    • C12Q2527/107Temperature of melting, i.e. Tm
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00Reactions of nucleic acids characterised by
    • C12Q2531/10Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/107Probe or oligonucleotide ligation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Bridges Or Land Bridges (AREA)
  • Application Of Or Painting With Fluid Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Materials For Medical Uses (AREA)
  • Joining Of Glass To Other Materials (AREA)
RU2020119664A 2017-11-22 2018-11-21 Методы асимметричной пцр RU2767061C2 (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US15/820,475 US10513730B2 (en) 2017-11-22 2017-11-22 Asymmetric PCR methods, primers and kits
US15/820,475 2017-11-22
PCT/EP2018/082086 WO2019101796A1 (en) 2017-11-22 2018-11-21 Asymmetric pcr methods

Publications (3)

Publication Number Publication Date
RU2020119664A RU2020119664A (ru) 2021-12-23
RU2020119664A3 true RU2020119664A3 (https=) 2021-12-23
RU2767061C2 RU2767061C2 (ru) 2022-03-16

Family

ID=64559658

Family Applications (1)

Application Number Title Priority Date Filing Date
RU2020119664A RU2767061C2 (ru) 2017-11-22 2018-11-21 Методы асимметричной пцр

Country Status (17)

Country Link
US (3) US10513730B2 (https=)
EP (1) EP3714065A1 (https=)
JP (1) JP7334154B2 (https=)
KR (1) KR20200088447A (https=)
CN (1) CN111727261B (https=)
AU (1) AU2018373766A1 (https=)
BR (1) BR112020009782A2 (https=)
CA (1) CA3082426A1 (https=)
IL (1) IL274638B2 (https=)
MX (1) MX2020005245A (https=)
MY (1) MY200344A (https=)
PH (1) PH12020550654A1 (https=)
RU (1) RU2767061C2 (https=)
SG (1) SG11202004284PA (https=)
TW (1) TWI822711B (https=)
WO (1) WO2019101796A1 (https=)
ZA (1) ZA202002570B (https=)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX2022000628A (es) 2019-07-19 2022-03-11 Safeguard Biosystems Holdings Ltd Deteccion de secuencias genomicas utilizando combinaciones de sondas, moleculas de sonda y arrays que comprenden las sondas para la deteccion especifica de organismos.
CN113046420B (zh) * 2019-12-26 2022-10-04 厦门大学 一种不对称扩增多个靶核酸的方法
CN113046421B (zh) * 2019-12-26 2022-09-30 厦门大学 一种不对称扩增靶核酸的方法
JP2024504966A (ja) 2021-01-20 2024-02-02 セイフガード バイオシステムズ ホールディングズ リミテッド 改善されたゲノム配列検出およびそのためのプローブ分子
CN116745434A (zh) 2021-01-20 2023-09-12 保障生物系统控股有限公司 基因组序列的改进的检测及其探针分子
CN115747328A (zh) * 2022-08-19 2023-03-07 福建师范大学 一种提高单链dna产量和改善产物比例的方法
CN121464225A (zh) * 2023-06-08 2026-02-03 威廉马歇莱思大学 被配置用于具有高动态范围的含核酸样品的高通量探询的方法和试剂盒
CN120138110B (zh) * 2025-03-14 2026-04-07 湖南大地同年生物科技有限公司 多重pcr检测反应中代替高温变性产生单链的方法和提高多重pcr产物液相杂交检测灵敏性和准确性的方法及配套的试剂盒

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5066584A (en) 1988-09-23 1991-11-19 Cetus Corporation Methods for generating single stranded dna by the polymerase chain reaction
US7435541B2 (en) * 2000-05-23 2008-10-14 Sequenom, Inc. Restriction enzyme genotyping
US6887664B2 (en) * 2000-06-06 2005-05-03 Applera Corporation Asynchronous primed PCR
AUPS076802A0 (en) * 2002-02-26 2002-03-21 Commonwealth Scientific And Industrial Research Organisation Selective nucleic acid amplification
WO2004068112A2 (en) * 2003-01-28 2004-08-12 Gorilla Genomics, Inc. Hairpin primer amplification
JP4322554B2 (ja) * 2003-05-19 2009-09-02 株式会社ニチレイフーズ Dna増幅反応の効率向上方法
CN100494399C (zh) * 2003-06-30 2009-06-03 清华大学 一种基于dna芯片的基因分型方法及其应用
CN1280422C (zh) 2004-08-26 2006-10-18 北京博奥生物芯片有限责任公司 一种不对称pcr扩增方法及其应用
ES2665690T3 (es) 2007-02-02 2018-04-26 Genera Biosystems Limited Generación de moléculas de ácido nucleico
CN103459612A (zh) * 2010-12-03 2013-12-18 布兰代斯大学 用于检测野生型群体中的核酸突变体的方法和试剂盒
WO2014052487A1 (en) * 2012-09-28 2014-04-03 Cepheid Two-primer pcr for microrna multiplex assay
WO2014160059A1 (en) * 2013-03-13 2014-10-02 Gen9, Inc. Compositions and methods for synthesis of high fidelity oligonucleotides
NO337188B1 (no) * 2013-04-08 2016-02-08 Niva COMPAS-PCR fremgangsmåte og fremgangsmåter for påvisning, identifisering eller overvåking av laksearter, kit, samt anvendelse av metoden og kit
CA2911303C (en) * 2013-05-07 2021-02-16 Micronics, Inc. Methods for preparation of nucleic acid-containing samples using clay minerals and alkaline solutions
CN104293794B (zh) 2014-09-24 2017-04-12 南方医科大学 一种与β‑淀粉样前体蛋白裂解酶1特异性结合的核酸适配子及其应用
EP3181700A1 (en) 2015-12-18 2017-06-21 Safeguard Biosystems Holdings Ltd. Three-dimensional polymer networks with channels situated therein
EP3199629A1 (en) 2016-01-30 2017-08-02 Safeguard Biosystems Holdings Ltd. Bead beating tube and method for extracting deoxyribonucleic acid and/or ribonucleic acid from microorganisms

Also Published As

Publication number Publication date
CA3082426A1 (en) 2019-05-31
US20200308629A1 (en) 2020-10-01
US20250290125A1 (en) 2025-09-18
US20190153508A1 (en) 2019-05-23
RU2020119664A (ru) 2021-12-23
AU2018373766A1 (en) 2020-05-28
IL274638B2 (en) 2025-03-01
IL274638A (en) 2020-06-30
CN111727261A (zh) 2020-09-29
IL274638B1 (en) 2024-11-01
KR20200088447A (ko) 2020-07-22
BR112020009782A2 (pt) 2020-10-13
TWI822711B (zh) 2023-11-21
JP2021503903A (ja) 2021-02-15
EP3714065A1 (en) 2020-09-30
MX2020005245A (es) 2020-08-24
RU2767061C2 (ru) 2022-03-16
MY200344A (en) 2023-12-20
SG11202004284PA (en) 2020-06-29
US10513730B2 (en) 2019-12-24
TW201925480A (zh) 2019-07-01
CN111727261B (zh) 2024-10-29
PH12020550654A1 (en) 2021-04-19
ZA202002570B (en) 2021-08-25
JP7334154B2 (ja) 2023-08-28
WO2019101796A1 (en) 2019-05-31

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