TWI822711B - 非對稱pcr方法、引子及套組 - Google Patents

非對稱pcr方法、引子及套組 Download PDF

Info

Publication number
TWI822711B
TWI822711B TW107141708A TW107141708A TWI822711B TW I822711 B TWI822711 B TW I822711B TW 107141708 A TW107141708 A TW 107141708A TW 107141708 A TW107141708 A TW 107141708A TW I822711 B TWI822711 B TW I822711B
Authority
TW
Taiwan
Prior art keywords
primer
region
pcr
sample
temperature
Prior art date
Application number
TW107141708A
Other languages
English (en)
Chinese (zh)
Other versions
TW201925480A (zh
Inventor
尼可拉斯 史密特
索妮雅 班納
侯格 克拉普
凱文 博毅特恩
Original Assignee
英商安全保護生技系統公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 英商安全保護生技系統公司 filed Critical 英商安全保護生技系統公司
Publication of TW201925480A publication Critical patent/TW201925480A/zh
Application granted granted Critical
Publication of TWI822711B publication Critical patent/TWI822711B/zh

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6848Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10Modifications characterised by
    • C12Q2525/131Modifications characterised by incorporating a restriction site
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10Modifications characterised by
    • C12Q2525/151Modifications characterised by repeat or repeated sequences, e.g. VNTR, microsatellite, concatemer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00Reactions demanding special reaction conditions
    • C12Q2527/101Temperature
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00Reactions demanding special reaction conditions
    • C12Q2527/107Temperature of melting, i.e. Tm
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00Reactions of nucleic acids characterised by
    • C12Q2531/10Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/107Probe or oligonucleotide ligation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Bridges Or Land Bridges (AREA)
  • Application Of Or Painting With Fluid Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Materials For Medical Uses (AREA)
  • Joining Of Glass To Other Materials (AREA)
TW107141708A 2017-11-22 2018-11-22 非對稱pcr方法、引子及套組 TWI822711B (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US15/820,475 US10513730B2 (en) 2017-11-22 2017-11-22 Asymmetric PCR methods, primers and kits
US15/820,475 2017-11-22

Publications (2)

Publication Number Publication Date
TW201925480A TW201925480A (zh) 2019-07-01
TWI822711B true TWI822711B (zh) 2023-11-21

Family

ID=64559658

Family Applications (1)

Application Number Title Priority Date Filing Date
TW107141708A TWI822711B (zh) 2017-11-22 2018-11-22 非對稱pcr方法、引子及套組

Country Status (17)

Country Link
US (3) US10513730B2 (https=)
EP (1) EP3714065A1 (https=)
JP (1) JP7334154B2 (https=)
KR (1) KR20200088447A (https=)
CN (1) CN111727261B (https=)
AU (1) AU2018373766A1 (https=)
BR (1) BR112020009782A2 (https=)
CA (1) CA3082426A1 (https=)
IL (1) IL274638B2 (https=)
MX (1) MX2020005245A (https=)
MY (1) MY200344A (https=)
PH (1) PH12020550654A1 (https=)
RU (1) RU2767061C2 (https=)
SG (1) SG11202004284PA (https=)
TW (1) TWI822711B (https=)
WO (1) WO2019101796A1 (https=)
ZA (1) ZA202002570B (https=)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX2022000628A (es) 2019-07-19 2022-03-11 Safeguard Biosystems Holdings Ltd Deteccion de secuencias genomicas utilizando combinaciones de sondas, moleculas de sonda y arrays que comprenden las sondas para la deteccion especifica de organismos.
CN113046420B (zh) * 2019-12-26 2022-10-04 厦门大学 一种不对称扩增多个靶核酸的方法
CN113046421B (zh) * 2019-12-26 2022-09-30 厦门大学 一种不对称扩增靶核酸的方法
JP2024504966A (ja) 2021-01-20 2024-02-02 セイフガード バイオシステムズ ホールディングズ リミテッド 改善されたゲノム配列検出およびそのためのプローブ分子
CN116745434A (zh) 2021-01-20 2023-09-12 保障生物系统控股有限公司 基因组序列的改进的检测及其探针分子
CN115747328A (zh) * 2022-08-19 2023-03-07 福建师范大学 一种提高单链dna产量和改善产物比例的方法
CN121464225A (zh) * 2023-06-08 2026-02-03 威廉马歇莱思大学 被配置用于具有高动态范围的含核酸样品的高通量探询的方法和试剂盒
CN120138110B (zh) * 2025-03-14 2026-04-07 湖南大地同年生物科技有限公司 多重pcr检测反应中代替高温变性产生单链的方法和提高多重pcr产物液相杂交检测灵敏性和准确性的方法及配套的试剂盒

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1629305A (zh) * 2004-08-26 2005-06-22 北京博奥生物芯片有限责任公司 一种不对称pcr扩增方法及其专用引物与应用

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5066584A (en) 1988-09-23 1991-11-19 Cetus Corporation Methods for generating single stranded dna by the polymerase chain reaction
US7435541B2 (en) * 2000-05-23 2008-10-14 Sequenom, Inc. Restriction enzyme genotyping
US6887664B2 (en) * 2000-06-06 2005-05-03 Applera Corporation Asynchronous primed PCR
AUPS076802A0 (en) * 2002-02-26 2002-03-21 Commonwealth Scientific And Industrial Research Organisation Selective nucleic acid amplification
WO2004068112A2 (en) * 2003-01-28 2004-08-12 Gorilla Genomics, Inc. Hairpin primer amplification
JP4322554B2 (ja) * 2003-05-19 2009-09-02 株式会社ニチレイフーズ Dna増幅反応の効率向上方法
CN100494399C (zh) * 2003-06-30 2009-06-03 清华大学 一种基于dna芯片的基因分型方法及其应用
ES2665690T3 (es) 2007-02-02 2018-04-26 Genera Biosystems Limited Generación de moléculas de ácido nucleico
CN103459612A (zh) * 2010-12-03 2013-12-18 布兰代斯大学 用于检测野生型群体中的核酸突变体的方法和试剂盒
WO2014052487A1 (en) * 2012-09-28 2014-04-03 Cepheid Two-primer pcr for microrna multiplex assay
WO2014160059A1 (en) * 2013-03-13 2014-10-02 Gen9, Inc. Compositions and methods for synthesis of high fidelity oligonucleotides
NO337188B1 (no) * 2013-04-08 2016-02-08 Niva COMPAS-PCR fremgangsmåte og fremgangsmåter for påvisning, identifisering eller overvåking av laksearter, kit, samt anvendelse av metoden og kit
CA2911303C (en) * 2013-05-07 2021-02-16 Micronics, Inc. Methods for preparation of nucleic acid-containing samples using clay minerals and alkaline solutions
CN104293794B (zh) 2014-09-24 2017-04-12 南方医科大学 一种与β‑淀粉样前体蛋白裂解酶1特异性结合的核酸适配子及其应用
EP3181700A1 (en) 2015-12-18 2017-06-21 Safeguard Biosystems Holdings Ltd. Three-dimensional polymer networks with channels situated therein
EP3199629A1 (en) 2016-01-30 2017-08-02 Safeguard Biosystems Holdings Ltd. Bead beating tube and method for extracting deoxyribonucleic acid and/or ribonucleic acid from microorganisms

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1629305A (zh) * 2004-08-26 2005-06-22 北京博奥生物芯片有限责任公司 一种不对称pcr扩增方法及其专用引物与应用

Also Published As

Publication number Publication date
CA3082426A1 (en) 2019-05-31
US20200308629A1 (en) 2020-10-01
US20250290125A1 (en) 2025-09-18
US20190153508A1 (en) 2019-05-23
RU2020119664A (ru) 2021-12-23
AU2018373766A1 (en) 2020-05-28
IL274638B2 (en) 2025-03-01
IL274638A (en) 2020-06-30
CN111727261A (zh) 2020-09-29
IL274638B1 (en) 2024-11-01
KR20200088447A (ko) 2020-07-22
BR112020009782A2 (pt) 2020-10-13
JP2021503903A (ja) 2021-02-15
EP3714065A1 (en) 2020-09-30
MX2020005245A (es) 2020-08-24
RU2020119664A3 (https=) 2021-12-23
RU2767061C2 (ru) 2022-03-16
MY200344A (en) 2023-12-20
SG11202004284PA (en) 2020-06-29
US10513730B2 (en) 2019-12-24
TW201925480A (zh) 2019-07-01
CN111727261B (zh) 2024-10-29
PH12020550654A1 (en) 2021-04-19
ZA202002570B (en) 2021-08-25
JP7334154B2 (ja) 2023-08-28
WO2019101796A1 (en) 2019-05-31

Similar Documents

Publication Publication Date Title
TWI822711B (zh) 非對稱pcr方法、引子及套組
US9057110B2 (en) Method for direct amplification from crude nucleic acid samples
CN103608467B (zh) 用于核酸的振荡扩增反应
CN107849603B (zh) 利用有限核苷酸组成的引物扩增
JP6970205B2 (ja) Dnaおよびrnaの同時濃縮を含むプライマー伸長標的濃縮およびそれに対する向上
JP5938348B2 (ja) 反応特異性の増加のための非標準塩基を含む増幅プライマー
CN104093850B (zh) 用于减少非特异性核酸扩增的方法和试剂盒
MX2007010875A (es) Oligonucleiotidos de especifidad dual y procesos que hacen uso de oligonucleotidos de especificidad dual.
JP2022518917A (ja) 核酸の検出方法及びプライマーの設計方法
Dada et al. Polymerase chain reaction
KR102106040B1 (ko) 자가혈당측정기를 활용한 표적핵산 검출방법
EP2935622A1 (en) Nucleic acid amplification method
KR20240152864A (ko) 고- 및 저-Tm 프라이머를 사용하는 베이스 2 초과의 지수적 베이스의 핵산 증폭
Kanu Jain et al. Polymerase Chain Reaction: A Powerful Diagnostic and Research Tool