MX2020005245A - Metodos de pcr asimetrica. - Google Patents

Metodos de pcr asimetrica.

Info

Publication number
MX2020005245A
MX2020005245A MX2020005245A MX2020005245A MX2020005245A MX 2020005245 A MX2020005245 A MX 2020005245A MX 2020005245 A MX2020005245 A MX 2020005245A MX 2020005245 A MX2020005245 A MX 2020005245A MX 2020005245 A MX2020005245 A MX 2020005245A
Authority
MX
Mexico
Prior art keywords
asymmetric pcr
pcr methods
asymmetric
primers
preparation
Prior art date
Application number
MX2020005245A
Other languages
English (en)
Inventor
Holger Klapproth
Sonja Bednar
Nicolaas Smit
Kevin Boynton
Original Assignee
Safeguard Biosystems Holdings Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Safeguard Biosystems Holdings Ltd filed Critical Safeguard Biosystems Holdings Ltd
Publication of MX2020005245A publication Critical patent/MX2020005245A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/131—Modifications characterised by incorporating a restriction site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/151—Modifications characterised by repeat or repeated sequences, e.g. VNTR, microsatellite, concatemer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/101—Temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/107—Temperature of melting, i.e. Tm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/107—Probe or oligonucleotide ligation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Bridges Or Land Bridges (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Materials For Medical Uses (AREA)
  • Joining Of Glass To Other Materials (AREA)
  • Application Of Or Painting With Fluid Materials (AREA)

Abstract

La descripción proporciona un método de amplificación por PCR asimétrica para la preparación de productos monocatenarios y cebadores y kits útiles para los mismos.
MX2020005245A 2017-11-22 2018-11-21 Metodos de pcr asimetrica. MX2020005245A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US15/820,475 US10513730B2 (en) 2017-11-22 2017-11-22 Asymmetric PCR methods, primers and kits
PCT/EP2018/082086 WO2019101796A1 (en) 2017-11-22 2018-11-21 Asymmetric pcr methods

Publications (1)

Publication Number Publication Date
MX2020005245A true MX2020005245A (es) 2020-08-24

Family

ID=64559658

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2020005245A MX2020005245A (es) 2017-11-22 2018-11-21 Metodos de pcr asimetrica.

Country Status (15)

Country Link
US (2) US10513730B2 (es)
EP (1) EP3714065A1 (es)
JP (1) JP7334154B2 (es)
KR (1) KR20200088447A (es)
AU (1) AU2018373766A1 (es)
BR (1) BR112020009782A2 (es)
CA (1) CA3082426A1 (es)
IL (1) IL274638A (es)
MX (1) MX2020005245A (es)
PH (1) PH12020550654A1 (es)
RU (1) RU2767061C2 (es)
SG (1) SG11202004284PA (es)
TW (1) TWI822711B (es)
WO (1) WO2019101796A1 (es)
ZA (1) ZA202002570B (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3999658A1 (en) 2019-07-19 2022-05-25 Safeguard Biosystems Holdings Ltd. Detection of genomic sequences using combinations of probes, probe molecules and arrays comprising the probes for the specific detection of organisms
CN113046420B (zh) * 2019-12-26 2022-10-04 厦门大学 一种不对称扩增多个靶核酸的方法
CN113046421B (zh) * 2019-12-26 2022-09-30 厦门大学 一种不对称扩增靶核酸的方法
WO2022157248A1 (en) 2021-01-20 2022-07-28 Safeguard Biosystems Holdings Ltd Improved detection of genomic sequences and probe molecules therefor
JP2024504966A (ja) 2021-01-20 2024-02-02 セイフガード バイオシステムズ ホールディングズ リミテッド 改善されたゲノム配列検出およびそのためのプローブ分子

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5066584A (en) 1988-09-23 1991-11-19 Cetus Corporation Methods for generating single stranded dna by the polymerase chain reaction
US7435541B2 (en) * 2000-05-23 2008-10-14 Sequenom, Inc. Restriction enzyme genotyping
US6887664B2 (en) * 2000-06-06 2005-05-03 Applera Corporation Asynchronous primed PCR
AUPS076802A0 (en) * 2002-02-26 2002-03-21 Commonwealth Scientific And Industrial Research Organisation Selective nucleic acid amplification
US20040259116A1 (en) * 2003-01-28 2004-12-23 Gorilla Genomics, Inc. Hairpin primer amplification
JP4322554B2 (ja) * 2003-05-19 2009-09-02 株式会社ニチレイフーズ Ｄｎａ増幅反応の効率向上方法
CN100494399C (zh) * 2003-06-30 2009-06-03 清华大学 一种基于dna芯片的基因分型方法及其应用
CN1280422C (zh) 2004-08-26 2006-10-18 北京博奥生物芯片有限责任公司 一种不对称pcr扩增方法及其应用
BRPI0807826A2 (pt) 2007-02-02 2014-08-05 Genera Biosystems Ltd " geração de moléculas de ácido nucleico ".
EP2646576B1 (en) * 2010-12-03 2015-10-28 Brandeis University Methods and kits for detecting nucleic acid mutants in wild-type populations
CA2886605A1 (en) * 2012-09-28 2014-04-03 Cepheid Two-primer pcr for microrna multiplex assay
WO2014160059A1 (en) * 2013-03-13 2014-10-02 Gen9, Inc. Compositions and methods for synthesis of high fidelity oligonucleotides
CA2911303C (en) * 2013-05-07 2021-02-16 Micronics, Inc. Methods for preparation of nucleic acid-containing samples using clay minerals and alkaline solutions
CN104293794B (zh) 2014-09-24 2017-04-12 南方医科大学 一种与β‑淀粉样前体蛋白裂解酶1特异性结合的核酸适配子及其应用
EP3181700A1 (en) 2015-12-18 2017-06-21 Safeguard Biosystems Holdings Ltd. Three-dimensional polymer networks with channels situated therein
US10036054B2 (en) 2016-01-30 2018-07-31 Safeguard Biosystems Holdings Ltd. Bead beating tube and method for extracting deoxyribonucleic acid and/or ribonucleic acid from microorganisms

Also Published As

Publication number Publication date
IL274638A (en) 2020-06-30
JP2021503903A (ja) 2021-02-15
US20190153508A1 (en) 2019-05-23
RU2767061C2 (ru) 2022-03-16
CN111727261A (zh) 2020-09-29
PH12020550654A1 (en) 2021-04-19
ZA202002570B (en) 2021-08-25
CA3082426A1 (en) 2019-05-31
RU2020119664A (ru) 2021-12-23
US20200308629A1 (en) 2020-10-01
KR20200088447A (ko) 2020-07-22
JP7334154B2 (ja) 2023-08-28
WO2019101796A1 (en) 2019-05-31
BR112020009782A2 (pt) 2020-10-13
TWI822711B (zh) 2023-11-21
SG11202004284PA (en) 2020-06-29
US10513730B2 (en) 2019-12-24
RU2020119664A3 (es) 2021-12-23
AU2018373766A1 (en) 2020-05-28
TW201925480A (zh) 2019-07-01
EP3714065A1 (en) 2020-09-30

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