CN111529470A

CN111529470A - Gel for removing acne and soothing allergy and preparation method thereof

Info

Publication number: CN111529470A
Application number: CN202010521452.XA
Authority: CN
Inventors: 秦昆明; 董自波; 高珣; 吉敬; 叶金水; 王嘉佳
Original assignee: Jiangsu Ocean University
Current assignee: Jiangsu Ocean University
Priority date: 2020-06-10
Filing date: 2020-06-10
Publication date: 2020-08-14
Anticipated expiration: 2040-06-10
Also published as: CN111529470B

Abstract

The invention discloses an acne-removing and allergy-relieving gel and a preparation method thereof, wherein the acne-removing and allergy-relieving gel is prepared from 1.9-6.74% of an acne-removing and allergy-relieving composition; 10-20% of glycerol; carbomer 0.5-1%; 0.5 to 1 percent of triethanolamine, 0.1 to 0.2 percent of methyl paraben and 71.06 to 87 percent of deionized water. The acne-removing allergy-relieving gel prepared by the invention is light yellow, glittering and translucent, proper in viscosity, good in stability and fresh and pleasant in smell; experimental results show that the gel has the effects of inhibiting propionibacterium acnes, removing acnes and scars and resisting skin allergy.

Description

Gel for removing acne and soothing allergy and preparation method thereof

Technical Field

The invention relates to an acne-removing allergy-relieving skin care product and a preparation method thereof, and in particular relates to an acne-removing allergy-relieving gel and a preparation method thereof.

Background

Acne, also known as face sore or pimple, is an inflammatory disease of skin follicles and commonly occurs in young people. The pox is formed by that sebaceous glands begin to secrete a large amount of grease under the action of male hormone, skin cutin metabolism is abnormal, skin pores are blocked, anaerobic bacteria begin to multiply in a large amount, and the skin has an inflammatory reaction. Brings great negative influence on life, psychology and social contact of patients. The causes of pox are explained by Chinese and western medicine, and the theory of traditional Chinese medicine considers that: acne refers to the condition that the heart, lung, spleen and stomach have heat, and the skin has poor blood circulation due to external infection of toxic factors, or the skin is overeated with fat, sweet, pungent and spicy food. The acne growing on the nose is caused by high stomach fire, the acne growing on the face is caused by liver and lung secretion and toxin expelling, the acne growing on the chin is endocrine dyscrasia, the acne growing on the mouth is accumulated by body toxin, the eating habit is improper, and the like. The western medicine theory considers that: one of the important causes of acne is the massive proliferation of propionibacterium acnes and other microorganisms, the production of lysolipases, the breakdown of sebum, the production of free acids, the resulting damaged rupture of the hair follicle wall, the entry of accumulated sebum into the surrounding dermal tissue, and the consequent clinical manifestations of lesions ranging from inflammatory papules to cysts.

Acne is a disease difficult to be cured radically, and needs to be controlled by long-term use of medicines, some products can be added with harmful components such as metronidazole, chloramphenicol, clindamycin and the like, and the antibiotics have remarkable treatment effect on the acne, but have great influence on skin after long-term use. The traditional Chinese medicine treatment mainly adopts traditional Chinese medicines such as scutellaria baicalensis, coptis chinensis, honeysuckle, witch hazel and the like, and is matched with certain active ingredients for oral administration and external application; the western medicine treatment mainly uses chemical substances such as hormones, antibiotics and the like, and the skin of a patient is greatly damaged by long-term use, so that the skin is stimulated and damaged. The living standard of people is continuously improved, the demand on cosmetics is increased, the demands on safety, efficacy and quality of products are gradually improved, and the traditional Chinese medicine cosmetics have small toxic and side effects, mild effects, stability and safety and are favored by more consumers. The comparative patent cn201410298553.x discloses a cosmetic containing aloe vera extract, natto extract and asiaticoside components, but the cosmetic has a weak inhibitory effect on propionibacterium acnes.

Disclosure of Invention

The purpose of the invention is as follows: aiming at the existing defects, the invention provides the compound acne-removing and allergy-relieving gel and the preparation method thereof, the compound acne-removing and allergy-relieving gel can overcome the problems of high skin irritation, more side effects, easy allergy and unsafety of western medicines, has the effects of clearing damp, relieving itching, clearing heat, cooling blood, promoting blood circulation, removing blood stasis, eliminating swelling, promoting granulation, conditioning and protecting facial skin, promoting cell metabolism and delaying senescence, and does not generate drug dependence.

The technical scheme is as follows: in order to achieve the purpose, the invention adopts the following technical scheme:

an acne-removing allergy-relieving gel is prepared from the following raw materials in percentage by weight:

1.9 to 6.74 percent of acne-removing and allergy-relieving composition;

carbomer 0.5-1%;

0.5 to 1 percent of triethanolamine;

10-20% of glycerol;

0.1 to 0.2 percent of methylparaben;

71.06-87% of deionized water.

The acne-removing and allergy-relieving composition is a multi-component mixture consisting of a centella extract, a bletilla extract, a honeysuckle extract, a algal polysaccharide extract, ascorbic acid, nicotinamide, a witch hazel extract, D-panthenol, potassium glycyrrhetate, bisabolol, tea tree oil and sodium hyaluronate.

0.05-0.1% of centella asiatica extract, 0.05-0.1% of bletilla striata extract, 0.05-0.1% of honeysuckle extract, 0.05-0.1% of algal polysaccharide extract, 0.1-1% of ascorbic acid, 1-3% of nicotinamide, 0.1-1% of hamamelis virginiana extract, 0.1-0.5% of D-panthenol, 0.1-0.2% of potassium glycyrrhetinate, 0.1-0.2% of bisabolol, 0.1-0.2% of tea tree oil, 0.1-0.24% of sodium hyaluronate, 0.5-1% of carbomer, 0.5-1% of triethanolamine, 10-20% of glycerol, 0.1-0.2% of methylparaben and 71.06-87% of deionized water.

The preparation method of the acne-removing and allergy-relieving gel provided by the invention comprises the following steps:

(1) putting the centella asiatica extract, the bletilla striata extract, the honeysuckle extract and the algal polysaccharide extract into a 100mL beaker according to the weight percentage, adding water, stirring until the mixture is dissolved, and filtering to obtain a traditional Chinese medicine extract solution;

(2) adding appropriate amount of water into ascorbic acid, nicotinamide, Hamamelis virginiana extractive solution and D-panthenol, and heating for dissolving; sequentially adding potassium glycyrrhizate, bisabolol and tea tree oil, and continuously stirring for dissolving; adding the traditional Chinese medicine extract solution prepared in the step (1), stopping heating and stirring after the traditional Chinese medicine extract solution is completely dissolved, slowly adding sodium hyaluronate, stirring for dissolving, standing and cooling, and adding methyl paraben to prepare a water phase;

(3) mixing carbomer with water according to the weight percentage, and swelling for 24 hours; after complete swelling, slowly adding triethanolamine dropwise, and stirring continuously to obtain transparent colloid; slowly adding glycerol, and stirring to obtain gel matrix;

(4) and (3) mixing the water phase prepared in the step (2) with the gel matrix prepared in the step (3), uniformly stirring, dropwise adding triethanolamine to adjust the pH value to 6-7, and adding water to a constant volume to obtain the gel.

The glycerin used in the invention has the effects of moistening skin, protecting skin and reducing water loss.

The triethanolamine used in the invention is neutralized with carboxyl of carbomer to form a stable macromolecular structure, so as to achieve the effects of thickening and moisturizing and have moisturizing and oil controlling effects on skin.

The ascorbic acid used in the invention can resist oxidation, improve the immunity of human bodies and improve the emergency protection capability of organisms.

The niacinamide used in the invention can prevent and treat the pellagra, has strong whitening and moisturizing effects, improves the whelk symptom, reduces skin inflammation and acne, and is a skin care component with high safety.

The witch hazel extract specially added in the invention can alleviate and stimulate skin, does not cause skin dryness, is deep and clean, enables skin to be more young and bright, effectively regulates pore relaxation, prevents and treats acne, has unique effects of aging resistance and the like.

The dipotassium glycyrrhizinate used in the invention can prevent allergy, resist bacteria, diminish inflammation, repair skin wound, inhibit bacterial growth and reduce free radicals, and has the characteristics of mild action, safety, stability and the like.

D-panthenol used in the present invention can promote epithelial tissue growth and wound healing as well as anti-inflammation.

The bisabolol used in the invention can resist bacteria, diminish inflammation, relieve itching and resist allergy, and is suitable for being added into cosmetics.

The sodium hyaluronate used in the invention can care skin, keep skin moist and glossy, and has the effects of moisturizing, nourishing, repairing and preventing skin injury, lubricating skin and forming film.

The tea tree oil used in the invention can diminish inflammation, inhibit bacteria, relieve itching, remove acnes, relieve allergy, keep the moisture and oil content balance of skin, promote the growth of tissue cells, help repair damaged cells and keep the skin moist and glossy.

The centella asiatica extract used in the invention has the effects of removing pockmarks, inhibiting bacteria, resisting oxidation, whitening and the like;

the bletilla striata extract used in the invention has the effects of stopping bleeding, resisting oxidation and aging, repairing damaged skin, removing pockmarks and pox marks, inhibiting bacterial growth and the like;

the honeysuckle extract used in the invention has the effects of resisting bacteria and diminishing inflammation, repairing damaged skin, fading scars and the like.

The algal polysaccharide used in the invention can be combined with skin protein to form a moisture-retaining gel, and can also form a protective film on the surface of the skin through the cooperation of water to prevent water evaporation.

Has the advantages that: the invention screens out the acne-removing and allergy-relieving component consisting of multiple components through experiments, and the experimental results show that the acne-removing and allergy-relieving component provided by the invention adopts 12 active components in the optimal weight ratio for compatibility and combination, has obvious acne-removing and allergy-relieving effects, and particularly has a good acne-inhibiting effect. The compatibility of all the components plays a good role in synergy.

In particular, the screening of the components shows that when the effective components are deficient in centella asiatica extract, hamamelis virginiana extract and tea tree oil components, the anti-propionibacterium acnes and the anti-allergic effect are obviously weakened.

According to the acne-removing and allergy-relieving composition, the acne-removing and allergy-relieving composition is prepared into a gel, and components dissolved in a water phase are dissolved in a gel matrix and can be slowly released, so that the effects of removing acne, relieving allergy and the like can be maintained for a long time.

Detailed Description

The present invention is further illustrated by the following specific examples, which are intended to be illustrative only and not limiting.

1.9 to 6.74 percent of acne-removing and allergy-relieving composition;

carbomer 0.5-1%;

0.5 to 1 percent of triethanolamine;

10-20% of glycerol;

0.1 to 0.2 percent of methylparaben;

71.06-87% of deionized water.

(5) putting the centella asiatica extract, the bletilla striata extract, the honeysuckle extract and the algal polysaccharide extract into a 100mL beaker according to the weight percentage, adding water, stirring until the mixture is dissolved, and filtering to obtain a traditional Chinese medicine extract solution;

(6) adding appropriate amount of water into ascorbic acid, nicotinamide, Hamamelis virginiana extractive solution and D-panthenol, and heating for dissolving; sequentially adding potassium glycyrrhizate, bisabolol and tea tree oil, and continuously stirring for dissolving; adding the traditional Chinese medicine extract solution prepared in the step (1), stopping heating and stirring after the traditional Chinese medicine extract solution is completely dissolved, slowly adding sodium hyaluronate, stirring for dissolving, standing and cooling, and adding methyl paraben to prepare a water phase;

(7) mixing carbomer with water according to the weight percentage, and swelling for 24 hours; after complete swelling, slowly adding triethanolamine dropwise, and stirring continuously to obtain transparent colloid; slowly adding glycerol, and stirring to obtain gel matrix;

(8) and (3) mixing the water phase prepared in the step (2) with the gel matrix prepared in the step (3), uniformly stirring, dropwise adding triethanolamine to adjust the pH value to 6-7, and adding water to a constant volume to obtain the gel.

The extraction methods of the centella asiatica extract, the bletilla striata extract, the honeysuckle extract and the algal polysaccharide extract used in the following examples are respectively as follows:

centella asiatica extract: cutting herba Centellae into segments, extracting with 75% ethanol under reflux for 2 times, adding 12 times of water for 1 time, and extracting for 1 hr; adding 10 times of water for the 2 nd time, and extracting for 1 h; filtering with three layers of gauze each time, mixing filtrates, concentrating the filtrate under reduced pressure until no alcohol smell is produced, and adding appropriate amount of water to disperse. Extracting with n-butanol for 2 times, mixing n-butanol layers, and concentrating under reduced pressure to dry to obtain herba Centellae extract.

And (3) common bletilla pseudobulb extract: slicing rhizoma Bletillae, adding water, reflux-extracting for 2 times, adding 12 times of water for 1 time, and extracting for 1.5 hr; adding 8 times of water for 2 times, and extracting for 1.5 hr; filtering with three layers of gauze each time, mixing filtrates, and concentrating the filtrate under reduced pressure to dry to obtain rhizoma Bletillae extract.

And (3) honeysuckle extract: reflux-extracting flos Lonicerae decoction pieces with water for 2 times, adding 15 times of water for 1 time, and extracting for 1.5 hr; adding 8 times of water for 2 times, and extracting for 1.5 hr; filtering with three layers of gauze each time, mixing filtrates, and concentrating the filtrate under reduced pressure to dry to obtain flos Lonicerae extract.

Algal polysaccharide extract: extracting Sargassum decoction pieces with water under reflux for 2 times, and extracting with 15 times of water for 1.5 hr for 1 time; adding 10 times of water for 2 times, and extracting for 1.5 hr; filtering with three layers of gauze each time, mixing filtrates, concentrating the filtrate under reduced pressure to material-liquid ratio of 1/10, adding ethanol until the alcohol concentration is 75%, standing overnight, and filtering to obtain polysaccharide crude extract. Separating and purifying the crude polysaccharide extract with Sephadex-G100 gel chromatography to obtain algal polysaccharide extract.

Example 1

The preparation method of the acne-removing and allergy-relieving gel comprises the following steps:

step (1): preparing a traditional Chinese medicine extract solution, namely putting 0.05 percent of centella extract, 0.05 percent of bletilla striata extract, 0.05 percent of honeysuckle extract and 0.05 percent of algal polysaccharide extract in a 100mL beaker by mass, adding water, stirring until the mixture is dissolved, and filtering to obtain a filtrate for later use;

step (2): taking 0.5% ascorbic acid, 1% nicotinamide, 0.5% hamamelis virginiana extract and 0.1% D-panthenol in percentage by mass, adding a proper amount of water, and heating for dissolving; then sequentially adding 0.1% of potassium glycyrrhizate, 0.1% of bisabolol and 0.1% of tea tree oil, and continuously stirring for dissolving; adding the traditional Chinese medicine extract solution obtained in the step (1), stopping heating and stirring after the traditional Chinese medicine extract solution is completely dissolved, slowly adding 0.1% sodium hyaluronate, stirring for dissolving, standing and cooling, and adding 0.1% methyl paraben to obtain a water phase;

and (3): mixing 0.5% carbomer with water, and swelling for 24 hr; after complete swelling, slowly dripping 0.5 percent triethanolamine, and continuously stirring to form transparent colloid; slowly adding 10% glycerol, and stirring to obtain gel matrix;

and (3) mixing the water phase prepared in the step (2) with the gel matrix prepared in the step (3), uniformly stirring, dropwise adding triethanolamine to adjust the pH value to 6, and adding water to a constant volume to obtain the gel.

Example 2

step (1): dissolving the traditional Chinese medicine extract, namely putting 0.08 percent of centella extract, 0.08 percent of bletilla extract, 0.08 percent of honeysuckle extract and 0.08 percent of algal polysaccharide extract in a 100ml beaker according to mass percentage, adding water, stirring until the mixture is dissolved, and filtering to obtain filtrate for later use;

step (2): taking 0.8% ascorbic acid, 2% nicotinamide, 0.8% hamamelis virginiana extract and 0.3% D-panthenol in percentage by mass, adding a proper amount of water, and heating for dissolving; then sequentially adding 0.2% of potassium glycyrrhizate, 0.2% of bisabolol and 0.2% of tea tree oil, and continuously stirring for dissolving; adding the Chinese medicinal extract solution, stopping heating and stirring after the Chinese medicinal extract solution is completely dissolved, slowly adding 0.2% sodium hyaluronate, stirring for dissolving, standing for cooling, and adding 0.2% methyl hydroxybenzoate to obtain water phase;

and (3): mixing 0.8% carbomer with water, and swelling for 24 hr; after complete swelling, slowly dripping 0.8% triethanolamine, and continuously stirring to obtain transparent colloid; slowly adding 15% glycerol, and stirring to obtain gel matrix;

and (3) mixing the water phase prepared in the step (2) with the gel matrix prepared in the step (3), uniformly stirring, dropwise adding triethanolamine to adjust the pH value to 7, and adding water to a constant volume to obtain the gel.

Example 3 preparation of Multi-component acne removing and allergy relieving gel

1. The formula of the multi-component acne-removing and allergy-relieving gel is given in the table 1, and the dosage of each substance is represented by mass fraction.

TABLE 1 Multi-component anti-acne and anti-allergy gel formula

Name of substance	Mass fraction/%
		Centella asiatica extract	0.05%
Bletilla striata extract	0.05%
		Honeysuckle extract	0.08%
Algal polysaccharide extract	0.08%
		Ascorbic acid	0.08%
Nicotinamide	2%
		Hamamelis virginianaExtract liquid	0.1%
D-panthenol	0.5%
		Glycyrrhiza diacid potassium salt	0.2%
Nipagin methyl ester	0.2%
		Bisabolol	0.2%
Tea tree oil	0.2%
		Hyaluronic acid sodium salt	0.2%
Carbomer	1%
		Triethanolamine	1%
Glycerol	15%
		Deionized water	79.06%

The preparation method of the multi-component acne-removing and allergy-relieving gel comprises the following steps: firstly, dissolving the traditional Chinese medicine extract in the formula shown in the table 1, putting the centella asiatica extract, the bletilla striata extract, the honeysuckle extract and the algal polysaccharide extract into a 100mL beaker, adding water, stirring until the mixture is dissolved, and filtering to obtain filtrate for later use; dissolving ascorbic acid, nicotinamide, Hamamelis virginiana extractive solution, D-panthenol in water under heating; sequentially adding potassium glycyrrhizate, bisabolol and tea tree oil, and continuously stirring for dissolving; adding the Chinese medicinal extract solution, stopping heating and stirring after the Chinese medicinal extract solution is completely dissolved, slowly adding sodium hyaluronate, stirring for dissolving, standing for cooling, and adding methyl hydroxybenzoate to obtain water phase;

mixing carbomer in formula table 1 with water, and swelling for 24 h; after complete swelling, slowly adding triethanolamine dropwise, and stirring continuously to obtain transparent colloid; slowly adding glycerol, and stirring to obtain gel matrix; mixing the water phase and the gel matrix, stirring uniformly, dropwise adding triethanolamine to adjust the pH value to 6, adding water to a constant volume, and preparing the acne-removing and allergy-relieving gel.

Comparative example 1

step (1): dissolving the traditional Chinese medicine extract, namely putting 0.08 percent of bletilla striata extract, 0.08 percent of honeysuckle extract and 0.08 percent of algal polysaccharide extract in a 100ml beaker by mass, adding water, stirring until the mixture is dissolved, and filtering to obtain filtrate for later use;

step (2): taking 0.8 percent of ascorbic acid, 2 percent of nicotinamide and 0.3 percent of D-panthenol by mass percent, adding a proper amount of water, and heating for dissolving; then sequentially adding 0.2% of potassium glycyrrhizate and 0.2% of bisabolol, and continuously stirring for dissolving; adding the Chinese medicinal extract solution, stopping heating and stirring after the Chinese medicinal extract solution is completely dissolved, slowly adding 0.2% sodium hyaluronate, stirring for dissolving, standing for cooling, and adding 0.2% methyl hydroxybenzoate to obtain water phase;

Example 4 acne treatment screening test: inhibition of Propionibacterium acnes

Sample preparation: the samples prepared in example 1, example 2 and comparative example 1 are taken as 1g each, and the comparative patent 201410298553X cosmetic is 2g, and the salicylic acid single component is 20 mg. A certain amount of sample is taken and respectively added into 50mL of deionized water or 50mL of 1, 3-butanediol, and the mixture is uniformly mixed for later use.

Strain culture: selecting 1-2 Propionibacterium acnes colonies, inoculating the Propionibacterium acnes colonies on an LB (Langerhans) plate culture medium, carrying out aerobic culture at 37 ℃ for 24 hours, washing the Propionibacterium acnes colonies with sterile normal saline, and putting washing liquid into a sterile test tube; the turbidimetry method is adopted, the turbiditube is shaken for 10-12 times before use, and the turbidimetry is firstly carried out to 3 x 10⁸/mL, diluted to 10 with physiological saline⁵-10⁶The volume is/mL for standby.

Inoculation of LB plate medium: dipping bacteria liquid with sterilized cotton stick, squeezing off excess bacteria liquid on tube wall, uniformly coating on LB plate culture medium, sweeping a circle along the edge of the plate, covering the plate, and drying for 2-3 min. Placing a sterile steel cup (diameter of 6mm) on a plate culture medium, filling the plate culture medium with a spare sample solution, placing the plate culture medium in a 37 ℃ aerobic culture for 24 hours, finding that no bacteria grow in the plate culture medium, and continuing the 37 ℃ aerobic culture for 24 hours to form a bacteriostatic zone.

Measuring the diameter of the bacteriostatic zone: taking an average value of diameter values of 3 groups of inhibition zones, and grading the antimicrobial susceptibility: the diameter of the antibacterial ring is more than 20mm, the diameter is high sensitivity, the diameter is 10-20mm, the diameter is medium sensitivity, and the diameter is less than 10mm, the diameter is drug resistance.

And (3) test results: see table 2:

TABLE 2 comparison of bacteriostatic results

Group of	Bacteriostatic diameter/mm
		EXAMPLE 1 sample set	18.7
EXAMPLE 2 sample set	21.3
		Comparative example 1	15.5
CN201410298553.X control group	15.8
		Salicylic acid group	13.5
Blank group	5.1

Therefore, the acne-removing and allergy-relieving gel prepared by the invention has an exact inhibition effect on propionibacterium acnes, and the effect is remarkably different from that of salicylic acid (p is less than 0.05). And showed better efficacy in inhibiting propionibacterium acnes than the cn201410298553.x control group and control example 1.

Example 5 anti-allergy screening assay

Taking mouse mastocytoma cells in logarithmic growth phase, and counting 0.6 × 10⁵Each well was suspended in 10% serum-containing RPMI-1640 medium, and the suspension was inoculated into a 24-well plate in a volume of 1 mL. After culturing for 48h, the medium was discarded, and 1ml of tyrode solution was added to each well, and the mixture was incubated with 5% CO₂The conditions were equilibrated for 10 minutes.

Each of the sample groups of Table 3 was added to 50. mu.L of a dilution of the active ingredient at the corresponding concentration. A negative control group (50. mu.L of Tyrode solution was added) and a positive control group (50. mu. L C48/80 stimulation was added) were set. The reaction was terminated after 60 min. The termination method comprises soaking in ice water bath at 0 deg.C for 10 min. Taking the reaction solution, and centrifuging for 10min at 2000r/min at 4 ℃ by a refrigerated centrifuge. The supernatant was collected and the amount of histamine released was measured by ELISA kit. The results are shown in Table 3.

Table 3 effect of antiallergic composition on histamine release amount ((n = 3)

Test grouping	Administration concentration (mg- mL）	Histamine concentration (ng- mL）
			Blank group	—	6.01±0.31
Model set	—	15.82±1.32
			Centella asiatica extract	0.05	11.36±0.89
Bletilla striata extract	0.05	12.71±1.92
			Honeysuckle extract	0.05	10.51±1.81
Hamamelis virginiana extractive solution	0.1	10.21±2.41
			Bisabolol	0.1	10.13±0.91
Tea tree oil	0.1	13.81±1.68
			Glycyrrhizic acid dipotassium salt	0.1	10.48±0.72
Nicotinamide	1	11.56±1.72
			D-panthenol	0.1	10.18±1.82
Hyaluronic acid sodium salt	0.1	12.01±1.15
			Comprises herba Centellae extract 0.05%, rhizoma Bletillae extract 0.05%, flos Lonicerae extract 0.05%, dipotassium glycyrrhizinate 0.1%, bisabolol 0.1%, and sodium hyaluronate 0.1% Mixed solution	0.1	9.39±0.83
Comprises herba Centellae extract 0.05%, rhizoma Bletillae extract 0.05%, flos Lonicerae extract 0.05%, radix Hamamelis mollis extractive solution 0.1%, dipotassium glycyrrhizinate 0.1%, and tea tree 0.1% Mixture of oil and 0.1% D-panthenol	0.1	8.73±0.92
			Comparative example 1	0.1	9.48±0.48
CN201410298553.X control group	0.1	9.61±0.58
			Example 1 sample	0.1	7.68±0.91^*#
Example 2 sample	0.1	7.18±0.73^*#

P < 0.05 in comparison with the model group and P < 0.05 in comparison with comparative example 1

As can be seen from the above table, in terms of inhibiting histamine production, examples 1 and 2, in which centella asiatica extract, bletilla striata extract, honeysuckle extract, algal polysaccharide extract, ascorbic acid, nicotinamide, hamamelis virginiana extract, D-panthenol, potassium glycyrrhizate, bisabolol, tea tree oil, and sodium hyaluronate were used in combination, showed better antiallergic effects than the control group of cn201410298553.x and control example 1 (lacking the cedarwood extract, hamamelis virginiana extract, and tea tree oil). And compared with other 6 or 7 components, the composition has better effect, which shows that the composition of 12 components provided by the invention has the best effect and has good complementary and synergistic effects.

Claims

1. The gel for removing acne and soothing allergy is characterized by being prepared from the following raw materials in percentage by weight:

1.9 to 6.74 percent of acne-removing and allergy-relieving composition;

carbomer 0.5-1%;

0.5 to 1 percent of triethanolamine;

10-20% of glycerol;

0.1 to 0.2 percent of methylparaben;

71.06-87% of deionized water.

2. The gel for removing acne and soothing allergy according to claim 1, wherein the composition for removing acne and soothing allergy is a mixture of centella asiatica extract, bletilla striata extract, honeysuckle extract, algal polysaccharide extract, ascorbic acid, niacinamide, witch hazel extract, D-panthenol, potassium glycyrrhizate, bisabolol, tea tree oil, and sodium hyaluronate.

3. The gel for removing acne and soothing allergy according to claim 2, which is prepared from the following raw materials in percentage by weight:

4. The preparation method of the gel for removing acne and soothing allergy is characterized by comprising the following steps:

(1) placing centella asiatica extract, bletilla striata extract, honeysuckle extract and algal polysaccharide extract in a 100mL beaker according to the weight percentage of the extract in the claim 3, adding water, stirring until the mixture is dissolved, and filtering to obtain a traditional Chinese medicine extract solution;

(2) dissolving ascorbic acid, nicotinamide, Hamamelis virginiana extractive solution, D-panthenol in water according to the weight percentage of claim 3 by heating; sequentially adding potassium glycyrrhizate, bisabolol and tea tree oil, and continuously stirring for dissolving; adding the traditional Chinese medicine extract solution obtained in the step (1), stopping heating and stirring after the traditional Chinese medicine extract solution is completely dissolved, slowly adding sodium hyaluronate, stirring for dissolving, standing and cooling, and adding methyl paraben to prepare a water phase;

(3) mixing carbomer with water, swelling, according to the weight percentage of claim 3; after complete swelling, slowly adding triethanolamine dropwise, and stirring continuously to obtain transparent colloid; slowly adding glycerol, and stirring to obtain gel matrix;

5. The preparation method of the gel for removing acne and soothing allergy according to claim 4, which is characterized in that,

the extraction method of the centella extract comprises the following steps: cutting herba Centellae into segments, extracting with 75% ethanol under reflux for 2 times, adding 10-16 times of water for 1 time, and extracting for 0.5-2 hr; adding 8-12 times of water for 2 times, and extracting for 0.5-2 hr; filtering with three layers of gauze each time, mixing filtrates, concentrating the filtrate under reduced pressure until no alcohol smell exists, and adding appropriate amount of water for dispersing; extracting with n-butanol for 2 times, mixing n-butanol layers, and concentrating under reduced pressure to dry to obtain herba Centellae extract.

6. The preparation method of the gel for removing acne and soothing allergy according to claim 4, wherein the extraction methods of the bletilla striata extracts are respectively as follows: slicing rhizoma Bletillae, adding water, reflux extracting for 2 times, adding 10-12 times of water for 1 time, and extracting for 0.5-1.5 hr; adding 8-10 times of water for 2 times, and extracting for 0.5-1.5 hr; filtering with three layers of gauze each time, mixing filtrates, and concentrating the filtrate under reduced pressure to dry to obtain rhizoma Bletillae extract.

7. The preparation method of the gel for removing acne and soothing allergy according to claim 4, wherein the honeysuckle extract is extracted by the following steps: reflux-extracting flos Lonicerae decoction pieces with water for 2 times, and adding 10-15 times of water for 1 st time for 0.5-1.5 hr; adding 8-12 times of water for 2 times, and extracting for 0.5-1.5 hr; filtering with three layers of gauze each time, mixing filtrates, and concentrating the filtrate under reduced pressure to dry to obtain flos Lonicerae extract.

8. The preparation method of the gel for removing acne and soothing allergy according to claim 4, wherein the extraction methods of the algal polysaccharide extract are respectively as follows: extracting Sargassum decoction pieces with water under reflux for 2 times, and extracting with 10-16 times of water for 1 time for 0.5-2 hr; adding 8-12 times of water for 2 times, and extracting for 0.5-2 hr; filtering with three layers of gauze each time, mixing filtrates, concentrating the filtrate under reduced pressure to material-liquid ratio of 1/10, adding ethanol until the alcohol concentration is 75%, standing overnight, and filtering to obtain polysaccharide crude extract; separating and purifying the crude polysaccharide extract with Sephadex-G100 gel chromatography to obtain algal polysaccharide extract.

9. Use of the acne-removing and allergy-relieving gel as defined in any one of claims 1 to 3 in preparation of antibacterial acne-removing and skin allergy-resisting cosmetics.