CN110559229A

CN110559229A - Night cream containing cannabidiol CBD nano-emulsion and preparation method thereof

Info

Publication number: CN110559229A
Application number: CN201910895708.0A
Authority: CN
Inventors: 李映波; 冯红; 宋小雪; 孙笠雯; 罗凡雅
Original assignee: Yunnan Chenjinwei Biotechnology Co Ltd
Current assignee: Yunnan Chenjinwei Biotechnology Co Ltd
Priority date: 2019-09-21
Filing date: 2019-09-21
Publication date: 2019-12-13

Abstract

the invention relates to night cream containing cannabidiol CBD nano-emulsion and a preparation method thereof, wherein the night cream is prepared from the following raw materials in percentage by mass: the composition comprises, by weight, 100% of sibiraca DG-3051-10%, 0.1-1% of callicarpa maxima extract, 0.1-1% of herba cepbalanoplosis segeti extract, 0.1-1% of campsis grandiflora extract, 1-15% of grape seed oil, 0.1-0.5% of phenoxyethanol, 0.1-3% of collagen, 0.05-1% of tocopherol, 1-3% of cetyl alcohol, 0.1-1% of xanthan gum, 1-5% of sodium lactate, 5-50% of CBD nano-emulsion and the balance of water. The cannabidiol CBD is dissolved in the oil phase, the cannabidiol CBD nanoemulsion with good stability and transdermal absorption is prepared, and then the synergistic effect of the mineral concentrate, the callicarpa macrophylla, the herba cepbalanoplosis segeti and the campsis grandiflora extract is matched, so that the damaged cells are repaired, the skin moisture is kept, the skin is relieved and whitened, the skin becomes more elastic and finer, the effect is obvious, and the application is safe.

Description

Night cream containing cannabidiol CBD nano-emulsion and preparation method thereof

Technical Field

The invention relates to the field of cosmetics, and particularly relates to night cream containing cannabidiol CBD nano-emulsion and a preparation method thereof

Background

the night cream is used for maintaining the damaged skin in the daytime at night after cleaning the skin at night, nourishing the skin, accelerating cell metabolism, enhancing the elasticity and smoothness of the skin, helping the skin to maintain a barrier, preventing the skin from being lack of water and supplementing nutrition. Most of the existing night cream products only have the effects of nourishing and moisturizing, a small part of night cream products have the repairing effect, the transdermal effect of the medicine is poor, the macromolecular raw materials are adopted, the night cream products can only stay on the surface layer of the skin, the skin is difficult to absorb, a large amount of effective substances are lost, and the requirements on deep care and nutrition supplement of the face cannot be met.

Cannabidiol (CBD) is an extract of dry matter of flowers and leaves of industrial cannabis sativa, is a non-addictive ingredient in cannabis sativa, is currently in the early stages of development and application, and has great potential and market for product development. The skin whitening and nourishing essence is applied to the field of cosmetics, can mainly whiten and repair damaged cells in the daytime, has pharmacological activities of resisting aging, resisting oxidation, deeply nourishing and tightening skin and the like, and can relieve the skin after being taken into a human body through the skin, so that the skin quality is improved from inside to outside, and the skin whitening and nourishing essence has a wide development and application prospect.

In recent years, cannabidiol has been widely used and has been reported. Patent CN 107982080 a discloses the use of cannabidiol or cannabis sativa extract in the preparation of whitening products, and finds that cannabidiol or cannabis sativa leaf extract can effectively inhibit the formation of melanin, and the prepared skin cream has whitening effect; patent CN 108685729a discloses a face cream and a preparation method thereof, and the prepared medicinal preparation can improve the absorption of human skin to effective components thereof, and realize the effects of soothing and nursing facial skin, whitening and beautifying, fading fine wrinkles, resisting oxidation and repairing, and delaying aging. Patent CN 107811904A discloses a

The anti-inflammatory and freckle-removing vanishing cream is added with the special cannabis sativa leaf extract rich in bisabolol and cannabidiol, has obvious anti-inflammatory and freckle-removing effects and the like, can exert stronger anti-inflammatory and freckle-removing effects for a longer time, and is synergistically enhanced in anti-aging and anti-wrinkle effects by the added vitamin E. Patent CN109939021A discloses an anti-aging composition, an essence stock solution and a preparation method thereof, the composition is composed of cannabidiol and oligopeptide-1, and under the synergistic effect of the cannabidiol and the oligopeptide-1, the composition can deeply nourish skin, improve cell anti-inflammatory reaction, promote cell metabolism, and further achieve the purpose of eliminating or relieving wrinkles.

however, because of the extremely poor water solubility of the cannabidiol CBD, the prior art mostly directly adds the cannabidiol CBD into the product, which causes low bioavailability and poor transdermal absorption, and limits the diversified application of the cannabidiol CBD to a certain extent. Therefore, the cannabidiol CBD needs to be specially treated, so that the solubility of the cannabidiol CBD is changed, the bioavailability is improved, and the maximum curative effect is achieved.

Disclosure of Invention

Aiming at the defects in the prior art, the invention provides night cream containing cannabidiol CBD nano-emulsion and mineral substances and a preparation method thereof, and particularly relates to a compound formula mainly containing cannabidiol CBD, so as to solve the problems in the background technology.

The invention is realized by the following technical scheme: night cream containing cannabidiol CBD nano-emulsion is prepared from the following raw materials in percentage by mass:

The sum of the above components is 100%.

the sibic DG-305 is an emulsifier, and belongs to a cosmetic grade product.

The grape seed oil is used as a sealing agent and belongs to a cosmetic grade product.

the phenoxyethanol is a preservative, and belongs to cosmetic products.

The collagen and the sodium lactate are used as moisturizers and belong to cosmetic products.

The cetyl alcohol and the xanthan gum are thickening agents and belong to cosmetic grade products.

The tocopherol is an antioxidant and belongs to cosmetic grade products.

the CBD nanoemulsion is prepared by the following method:

(1) preparing materials according to the following mass ratio:

the sum of the components is 100 percent;

(2) Heating oil phase shea butter, MCT oil and soybean oil at 30-45 ℃ for 10-30 min, adding cannabidiol, stirring at 30-45 ℃ until the cannabidiol is completely dissolved, and cooling to room temperature to obtain an oil solution;

(3) Stirring the mineral concentrated solution and water until the mineral concentrated solution and the water are completely mixed and dissolved to obtain a water-soluble substance;

(4) Heating and dissolving an emulsifier EL-40, Tween-80, lecithin, poloxamer and triglycerin at 40-50 ℃, cooling to room temperature, adding glycerol, uniformly mixing, dropwise adding an oil soluble substance, dropwise adding a water soluble substance, stirring while dropwise adding, rotating speed of 400-600 r/min, and obtaining the CBD nano-emulsion when the solution turns turbid from clear to clear.

The Callicarpa macrophylla extract is extracted from leaves or tender branches with leaves of Callicarpa macrophylla, is of Verbenaceae, contains sitosterol and apigenin, and has effects of removing blood stasis, stopping bleeding, relieving swelling and pain, etc.

the callicarpa macrophylla extract is prepared by the following steps: crushing the dried callicarpa macrocarpa, sieving with a 20-mesh sieve, adding 75-90% ethanol according to the feed liquid mass ratio of 1:6-10, carrying out ultrasonic extraction for 3 times and 1-3 h/time, filtering, taking filtrate, combining the 3 times of filtrate, carrying out suction filtration after overnight in an ice bath to obtain an extracting solution, carrying out reduced pressure recovery on the ethanol at room temperature, and concentrating at 40-50 ℃ to obtain a thick paste to obtain the callicarpa macrocarpa extract.

The herba Cephalanoploris extract is extracted from tender stem and leaf of herba Cephalanoploris, is perennial herb of Compositae, contains multiple chemical components such as protocatechin, chlorogenic acid, beta-sitosterol, etc., and has antibacterial, tranquilizing, blood cooling, hemostasis, blood stasis dispelling, and repercussive effects.

The herba Cirsii extract is prepared by the following steps: crushing dried common cephalanoplos herb, sieving the powder by a sieve of 25-40 meshes, adding 75% ethanol by mass according to 8-15 times of the mass of the common cephalanoplos herb powder, performing reflux extraction for 1-3 times, each time for 30-120 minutes, filtering, combining extracting solutions, performing overnight cooling in an ice bath, taking supernate, recovering ethanol under reduced pressure at the conditions of 30-60 ℃, rotation speed of 30-60 r/min and 0.06-0.08 Mpa, and concentrating the supernate to be thick paste at 45 ℃ to obtain the common cephalanoplos herb extract.

The campsis grandiflora extract is extracted from campsis grandiflora of Bignoniaceae, and has the effects of promoting blood circulation, removing blood stasis, cooling blood, dispelling pathogenic wind, relieving skin itching, treating acne and the like.

the campsis grandiflora extract is prepared by the following steps: pulverizing flos Campsis, extracting with distilled water by conventional cold soaking, warm soaking, and ultrasonic wave, filtering, collecting filtrate, and spray drying or freeze drying to obtain flos Campsis extract; wherein the adding amount of the distilled water is 10-50 times of the mass of the trumpetcreeper pollen, the cold soaking is carried out for 1-10 days at room temperature, the warm soaking is carried out for 1-3 hours at 40-60 ℃, and the extraction times are 1-3 times; the ultrasonic-assisted extraction is carried out at the temperature of 40-60 ℃, the ultrasonic power of 100-650W, the ultrasonic frequency of 19-23 KHz and the ultrasonic output power of 70-100%, the extraction times are 1-3, and each time lasts for 30-60 minutes; pre-freezing for 3-16 hours at the condensation temperature of-20 to-50 ℃ in vacuum freeze drying, wherein the vacuum degree is 5-20 KPa; the air inlet temperature of spray drying is 100-300 ℃, and the air outlet temperature is 30-140 ℃.

The cannabidiol CBD is a commercial product extracted from industrial cannabis sativa with THC lower than 0.3%, belongs to a non-addictive active substance, has the main effect of repairing damaged cells, and has the effects of resisting inflammation, allergy, aging, bacteria, oxidation and ultraviolet rays and the like.

The mineral concentrated solution is a commercial product, contains various natural mineral elements, is easy to be absorbed by human bodies, is convenient to use, can be used for supplementing minerals to skin, and is a safe, sanitary and healthy inorganic salt nutrient.

The castor oil polyoxyethylene ether EL-40, the Tween-80, the lecithin and the poloxamer are used as emulsifiers and belong to commercially available cosmetic products.

The glycerol and the triglycerol are used as the auxiliary emulsifier and belong to commercially available cosmetic products.

The shea butter, MCT oil and soybean oil are used as oil phases and belong to commercially available cosmetic-grade products.

The night cream containing cannabidiol CBD nano-emulsion and mineral substances provided by the invention is prepared by the following steps:

Preparing materials according to the following mass ratio:

The sum of the components is 100 percent;

adding a proper amount of water into a moisturizing agent collagen and sodium lactate, fully dissolving at 40-60 ℃, adding a callicarpa bodinieri extract, a herba cepbalanoplosis segeti extract and a campsis grandiflora extract, stirring to promote dissolution, adding a thickening agent cetyl alcohol and xanthan gum, fully dissolving, maintaining the temperature at 35-45 ℃, performing ultrasonic treatment under the conditions that the ultrasonic power is 10-20%, the ultrasonic starting time is 5-10 s, the ultrasonic closing time is 5-10 s and the total duration is 5-15 min to obtain a material B;

secondly, mixing grape seed oil serving as a sealing agent, tocopherol serving as an antioxidant and a proper amount of water, heating at 40-60 ℃ for 5-15 min until the mixture is fully dissolved, cooling at room temperature, adding an emulsifier, namely, sibirak DG-305, fully stirring and uniformly mixing at 30-40 ℃, dropwise adding a preservative, namely, phenoxyethanol, and fully stirring at the stirring speed of 300-500 r/min to obtain a material C;

And thirdly, adding the material B into the material C, heating for 5-10 min at 30-40 ℃, adding the CBD nano-emulsion, and fully stirring and mixing for 5-15 min to obtain the night cream containing the cannabidiol CBD nano-emulsion.

The invention has the following advantages and effects: by adopting the scheme, the cannabidiol CBD is firstly dissolved in the oil phase, and is prepared into the cannabidiol CBD nanoemulsion with good stability and transdermal absorbability, and then the synergistic effect of the mineral concentrate, the callicarpa macrophylla, the herba cepbalanoplosis segeti and the campsis grandiflora extracts is matched, so that the damaged cells are repaired, the skin moisture is kept, the skin is relieved and whitened, the skin becomes more elastic and finer, the effect is obvious, and the medicine is safe to take.

Detailed description of the invention

The present invention will be further described with reference to the following examples.

Example 1

night cream containing cannabidiol CBD nano-emulsion and a preparation method thereof, the night cream comprises the following steps:

(1) Preparing night cream from the following raw materials in percentage by mass:

(2) Adding a proper amount of water into a moisturizing agent collagen and sodium lactate, fully dissolving in a water bath kettle at 60 ℃, adding a callicarpa bodinieri extract, a herba cepbalanoplosis segeti extract and a campsis grandiflora extract, stirring for promoting dissolution, adding a thickening agent cetyl alcohol and xanthan gum, fully dissolving, maintaining the temperature at 35 ℃, performing ultrasonic treatment under the conditions that the ultrasonic power is 20%, the ultrasonic starting time is 5s, the ultrasonic shutdown time is 10s, and the total duration time is 7min to obtain a material B;

(3) Mixing grape seed oil serving as a sealing agent, tocopherol serving as an antioxidant and a proper amount of water, heating at 60 ℃ for 5min to fully dissolve, cooling at room temperature, adding an emulsifier, namely, sibirak DG-305, fully stirring and uniformly mixing at 30 ℃, dropwise adding a preservative, namely, phenoxyethanol, and fully stirring at the stirring speed of 300r/min to obtain a material C;

(4) Adding the material B into the material C, heating at 30 deg.C for 10min, adding CBD nanoemulsion, stirring and mixing for 5min to obtain night cream containing cannabidiol CBD nanoemulsion.

The CBD nanoemulsion is prepared by the following method:

(1) preparing materials according to the following mass ratio:

(2) heating oil phase Shea butter, MCT oil and soybean oil at 30 deg.C for 30min, adding cannabidiol, stirring at 30 deg.C until cannabidiol is completely dissolved, and cooling to room temperature to obtain oil solution;

(4) Heating and dissolving the emulsifier EL-40, Tween-80, lecithin, poloxamer and triglycerin at 40 ℃ to dissolve, cooling to room temperature, adding glycerol, mixing uniformly, dropwise adding an oil soluble substance, dropwise adding a water soluble substance, stirring while dropwise adding, rotating speed of 400r/min, and obtaining the CBD nano-emulsion when the solution turns turbid from clear to clear from turbid.

the callicarpa macrophylla extract is prepared by the following steps: crushing the dried callicarpa macrocarpa, sieving with a 20-mesh sieve, adding 75% ethanol according to the feed liquid mass ratio of 1:8, carrying out ultrasonic extraction for 3 times and 2 h/time, filtering, taking filtrate, combining the 3 times of filtrate, carrying out suction filtration for obtaining an extracting solution after overnight cooling in an ice bath, carrying out reduced pressure recovery on the ethanol at room temperature, and concentrating at 40 ℃ to obtain a thick paste, thus obtaining the callicarpa macrocarpa extract.

the herba Cirsii extract is prepared by the following steps: pulverizing dried herba Cephalanoploris, sieving with 25 mesh sieve, adding 75% ethanol 8 times the weight of herba Cephalanoploris powder, reflux extracting for 3 times (30 min each time), filtering, mixing extractive solutions, standing overnight in ice bath, collecting supernatant, recovering ethanol at 30 deg.C under 30 rpm/min and 0.06Mpa under reduced pressure, and concentrating at 45 deg.C to obtain herba Cephalanoploris extract.

The campsis grandiflora extract is prepared by the following steps: pulverizing flos Campsis, extracting with distilled water by conventional cold soaking, warm soaking or ultrasonic wave, filtering, collecting filtrate, and spray drying or freeze drying to obtain flos Campsis extract; wherein, the adding amount of the distilled water is 35 times of the weight of the trumpetcreeper pollen, the cold soaking is to soak for 10 days at room temperature, the warm soaking is to soak for 2 hours at 55 ℃, and the extraction times are 2 times; the ultrasonic auxiliary extraction is carried out at the temperature of 45 ℃, the ultrasonic power of 550W, the ultrasonic frequency of 21KHz and the ultrasonic output power of 85 percent, the extraction times are 2 times, and each time lasts for 35 minutes; pre-freezing for 10 hours at the condensation temperature of-35 ℃ and the vacuum degree of 10KPa in vacuum freeze drying; the air inlet temperature of spray drying is 250 ℃, and the air outlet temperature is 120 ℃.

example 2

night cream containing cannabidiol CBD nano-emulsion and a preparation method thereof, the night cream comprises the following steps: (1) preparing night cream from the following raw materials in percentage by mass:

(2) adding a proper amount of water into a moisturizing agent collagen and sodium lactate, fully dissolving in a water bath kettle at 40 ℃, adding a callicarpa bodinieri extract, a herba cepbalanoplosis segeti extract and a campsis grandiflora extract, stirring for promoting dissolution, adding a thickening agent cetyl alcohol and xanthan gum, fully dissolving, maintaining the temperature at 40 ℃, performing ultrasonic treatment under the conditions that the ultrasonic power is 10%, the ultrasonic starting time is 10s, the ultrasonic shutdown time is 5s, and the total duration time is 5min to obtain a material B;

(3) Mixing grape seed oil serving as a sealing agent, tocopherol serving as an antioxidant and a proper amount of water, heating at 40 ℃ for 10min to fully dissolve, cooling at room temperature, adding an emulsifier, namely, sibirak DG-305, fully stirring and uniformly mixing at 35 ℃, dropwise adding a preservative, namely, phenoxyethanol, and fully stirring at the stirring speed of 500r/min to obtain a material C;

(4) Adding the material B into the material C, heating at 40 deg.C for 5min, adding CBD nanoemulsion, stirring and mixing thoroughly for 15min to obtain night cream containing cannabidiol CBD nanoemulsion.

the CBD nanoemulsion is prepared by the following method:

(1) Preparing materials according to the following mass ratio:

(2) heating oil phase Shea butter, MCT oil and soybean oil at 45 deg.C for 10min, adding cannabidiol, stirring at 45 deg.C until cannabidiol is completely dissolved, and cooling to room temperature to obtain oil solution;

(4) Heating and dissolving the emulsifier EL-40, Tween-80, lecithin, poloxamer and triglycerin at 50 ℃ to dissolve, cooling to room temperature, adding glycerol, mixing uniformly, dropwise adding an oil soluble substance, dropwise adding a water soluble substance, stirring while dropwise adding, rotating speed of 400r/min, and obtaining the CBD nano-emulsion when the solution turns turbid from clear to clear from turbid.

The callicarpa macrophylla extract is prepared by the following steps: crushing the dried callicarpa macrocarpa, sieving with a 20-mesh sieve, adding 90% ethanol according to the feed liquid mass ratio of 1:8, carrying out ultrasonic extraction for 3 times and 3 h/time, filtering, taking filtrate, combining the 3 times of filtrate, carrying out suction filtration for obtaining an extracting solution after overnight cooling in an ice bath, carrying out reduced pressure recovery on the ethanol at room temperature, and concentrating at 45 ℃ to obtain a thick paste, thus obtaining the callicarpa macrocarpa extract.

The herba Cirsii extract is prepared by the following steps: pulverizing dried herba Cephalanoploris, sieving with 30 mesh sieve, adding 75% ethanol 10 times the weight of herba Cephalanoploris powder, reflux extracting for 1 time (60 min each time), filtering, mixing extractive solutions, standing overnight in ice bath, collecting supernatant, recovering ethanol at 35 deg.C under 40 r/min and 0.08Mpa under reduced pressure, and concentrating at 45 deg.C to obtain herba Cephalanoploris extract.

The campsis grandiflora extract is prepared by the following steps: pulverizing flos Campsis, extracting with distilled water by conventional cold soaking, warm soaking or ultrasonic wave, filtering, collecting filtrate, and spray drying or freeze drying to obtain flos Campsis extract; wherein, the adding amount of the distilled water is 25 times of the weight of the trumpetcreeper pollen, the cold soaking is to soak for 5 days at room temperature, the warm soaking is to soak for 1 hour at 45 ℃, and the extraction times are 3 times; the ultrasonic-assisted extraction is carried out at 50 ℃, 500W of ultrasonic power, 21KHz of ultrasonic frequency and 90 percent of ultrasonic output power, the extraction times are 1 time, and each time lasts for 50 minutes; pre-freezing for 6 hours at-45 ℃ in the condensation temperature of vacuum freeze drying, wherein the vacuum degree is 10 KPa; the air inlet temperature of spray drying is 300 ℃, and the air outlet temperature is 120 ℃.

Example 3

(2) Adding a proper amount of water into a moisturizing agent collagen and sodium lactate, fully dissolving at 45 ℃, adding a callicarpa bodinieri extract, a herba cepbalanoplosis segeti extract, stirring to promote dissolution, then adding a thickening agent cetyl alcohol and xanthan gum, fully dissolving, maintaining the temperature at 45 ℃, performing ultrasonic treatment under the conditions that the ultrasonic power is 15%, the ultrasonic starting time is 7s, the ultrasonic closing time is 10s, and the total duration is 15min to obtain a material B;

(3) Mixing grape seed oil serving as a sealing agent, tocopherol serving as an antioxidant and a proper amount of water, heating at 45 ℃ for 7min until the mixture is fully dissolved, cooling at room temperature, adding an emulsifier, namely, sibirak DG-305, fully stirring and uniformly mixing at 40 ℃, dropwise adding a preservative, namely, phenoxyethanol, and fully stirring at the stirring speed of 400r/min to obtain a material C;

(4) adding the material B into the material C, heating at 35 deg.C for 7min, adding CBD nanoemulsion, stirring and mixing for 8min to obtain night cream containing cannabidiol CBD nanoemulsion.

The CBD nanoemulsion is prepared by the following method:

(1) preparing materials according to the following mass ratio:

(2) heating oil phase Shea butter, MCT oil and soybean oil at 35 deg.C for 20min, adding cannabidiol, stirring at 35 deg.C until cannabidiol is completely dissolved, and cooling to room temperature to obtain oil solution;

(4) Heating and dissolving the emulsifier EL-40, Tween-80, lecithin, poloxamer and triglycerin at 45 ℃ to dissolve, cooling to room temperature, adding glycerol, mixing uniformly, dropwise adding an oil soluble substance, dropwise adding a water soluble substance, stirring while dropwise adding, rotating speed of 500r/min, and obtaining the CBD nanoemulsion when the solution turns turbid from clear to clear.

the callicarpa macrophylla extract is prepared by the following steps: crushing the dried callicarpa macrocarpa, sieving with a 20-mesh sieve, adding 85% ethanol according to the feed liquid mass ratio of 1:8, carrying out ultrasonic extraction for 3 times and 1 h/time, filtering, taking filtrate, combining the 3 times of filtrate, carrying out suction filtration for obtaining an extracting solution after overnight cooling in an ice bath, carrying out reduced pressure recovery on the ethanol at room temperature, and concentrating at 50 ℃ to obtain a thick paste to obtain the callicarpa macrocarpa extract.

The herba Cirsii extract is prepared by the following steps: pulverizing dried herba Cephalanoploris, sieving with 35 mesh sieve, adding 75% ethanol 15 times the weight of herba Cephalanoploris powder, reflux extracting for 1 time (90 min each time), filtering, mixing extractive solutions, standing overnight in ice bath, collecting supernatant, recovering ethanol at 45 deg.C under 50 rpm/min and 0.065Mpa under reduced pressure, and concentrating at 45 deg.C to obtain herba Cephalanoploris extract.

The campsis grandiflora extract is prepared by the following steps: pulverizing flos Campsis, extracting with distilled water by conventional cold soaking, warm soaking or ultrasonic wave, filtering, collecting filtrate, and spray drying or freeze drying to obtain flos Campsis extract; wherein, the adding amount of the distilled water is 50 times of the weight of the trumpetcreeper pollen, the cold soaking is to soak for 1 day at room temperature, the warm soaking is to soak for 3 hours at 50 ℃, and the extraction times are 1 time; the ultrasonic-assisted extraction is carried out at the temperature of 60 ℃, the ultrasonic power of 100W, the ultrasonic frequency of 23KHz and the ultrasonic output power of 100 percent, and the extraction times are 3 times and 40 minutes each time; pre-freezing for 16 hours at the condensation temperature of-50 ℃ in vacuum freeze drying, wherein the vacuum degree is 20 KPa; the air inlet temperature of spray drying is 220 ℃, and the air outlet temperature is 30 ℃.

Example 4

(2) Adding a proper amount of water into a moisturizing agent collagen and sodium lactate, fully dissolving at 55 ℃, adding a callicarpa bodinieri extract, a herba cepbalanoplosis segeti extract, stirring to promote dissolution, then adding a thickening agent cetyl alcohol and xanthan gum, fully dissolving, maintaining the temperature at 35 ℃, performing ultrasonic treatment under the conditions that the ultrasonic power is 10%, the ultrasonic starting time is 5s, the ultrasonic closing time is 7s, and the total duration is 10min to obtain a material B;

(3) Mixing grape seed oil serving as a sealing agent, tocopherol serving as an antioxidant and a proper amount of water, heating at 55 ℃ for 10min to fully dissolve, cooling at room temperature, adding an emulsifier, namely, sibirak DG-305, fully stirring and uniformly mixing at 35 ℃, dropwise adding a preservative, namely, phenoxyethanol, and fully stirring at the stirring speed of 400r/min to obtain a material C;

(4) Adding the material B into the material C, heating at 35 deg.C for 7min, adding CBD nanoemulsion, stirring and mixing for 10min to obtain night cream containing cannabidiol CBD nanoemulsion.

the CBD nanoemulsion is prepared by the following method:

(1) preparing materials according to the following mass ratio:

(2) Heating oil phase Shea butter, MCT oil and soybean oil at 40 deg.C for 25min, adding cannabidiol, stirring at 40 deg.C until cannabidiol is completely dissolved, and cooling to room temperature to obtain oil solution;

(4) heating and dissolving the emulsifier EL-40, Tween-80, lecithin, poloxamer and triglycerin at 40 ℃ to dissolve, cooling to room temperature, adding glycerol, mixing uniformly, dropwise adding an oil soluble substance, dropwise adding a water soluble substance, stirring while dropwise adding, rotating speed of 450r/min, and obtaining the CBD nanoemulsion when the solution turns turbid from clear to clear.

The callicarpa macrophylla extract is prepared by the following steps: crushing the dried callicarpa macrocarpa, sieving with a 20-mesh sieve, adding 80% ethanol according to the feed liquid mass ratio of 1:8, carrying out ultrasonic extraction for 3 times and 1 h/time, filtering, taking filtrate, combining the filtrate for 3 times, carrying out suction filtration for overnight in an ice bath to obtain an extracting solution, carrying out reduced pressure recovery on the ethanol at room temperature, and concentrating at 45 ℃ to obtain a thick paste, thus obtaining the callicarpa macrocarpa extract.

the herba Cirsii extract is prepared by the following steps: pulverizing dried herba Cephalanoploris, sieving with 40 mesh sieve, adding 75% ethanol 15 times the weight of herba Cephalanoploris powder, reflux extracting for 2 times (120 min each time), filtering, mixing extractive solutions, standing overnight in ice bath, collecting supernatant, recovering ethanol at 50 deg.C, rotation speed of 60 r/min and 0.075Mpa under reduced pressure, and concentrating at 45 deg.C to obtain herba Cephalanoploris extract.

The campsis grandiflora extract is prepared by the following steps: pulverizing flos Campsis, extracting with distilled water by conventional cold soaking, warm soaking or ultrasonic wave, filtering, collecting filtrate, and spray drying or freeze drying to obtain flos Campsis extract; wherein, the adding amount of the distilled water is 10 times of the weight of the trumpetcreeper pollen, the cold soaking is to soak for 10 days at room temperature, the warm soaking is to soak for 3 hours at 40 ℃, and the extraction times are 1 time; the ultrasonic-assisted extraction is carried out at the temperature of 55 ℃, the ultrasonic power of 650W, the ultrasonic frequency of 19KHz and the ultrasonic output power of 85 percent, the extraction times are 1 time, and each time lasts for 30 minutes; pre-freezing for 5 hours at the condensation temperature of-20 ℃ in vacuum freeze drying, wherein the vacuum degree is 10 KPa; the air inlet temperature of spray drying is 300 ℃, and the air outlet temperature is 140 ℃.

Example 5

(2) adding a proper amount of water into a moisturizing agent collagen and sodium lactate, fully dissolving at 45 ℃, adding a callicarpa bodinieri extract, a herba cepbalanoplosis segeti extract, stirring to promote dissolution, then adding a thickening agent cetyl alcohol and xanthan gum, fully dissolving, maintaining the temperature at 45 ℃, performing ultrasonic treatment under the conditions that the ultrasonic power is 15%, the ultrasonic starting time is 10s, the ultrasonic closing time is 5s, and the total duration is 15min to obtain a material B;

(3) Mixing grape seed oil serving as a sealing agent, tocopherol serving as an antioxidant and a proper amount of water, heating at 50 ℃ for 10min to fully dissolve, cooling at room temperature, adding an emulsifier, namely, sibirak DG-305, fully stirring and uniformly mixing at 40 ℃, dropwise adding a preservative, namely, phenoxyethanol, and fully stirring at the stirring speed of 400r/min to obtain a material C;

(4) adding the material B into the material C, heating at 35 deg.C for 6min, adding CBD nanoemulsion, stirring and mixing thoroughly for 15min to obtain night cream containing cannabidiol CBD nanoemulsion.

the CBD nanoemulsion is prepared by the following method:

(1) preparing materials according to the following mass ratio:

(2) Heating oil phase Shea butter, MCT oil and soybean oil at 42 deg.C for 28min, adding cannabidiol, stirring at 42 deg.C until cannabidiol is completely dissolved, and cooling to room temperature to obtain oil solution;

The callicarpa macrophylla extract is prepared by the following steps: crushing the dried callicarpa macrocarpa, sieving with a 20-mesh sieve, adding 75% ethanol according to the feed liquid mass ratio of 1:8, carrying out ultrasonic extraction for 3 times and 3 h/time, filtering, taking filtrate, combining the 3 times of filtrate, carrying out suction filtration for obtaining an extracting solution after overnight cooling in an ice bath, carrying out reduced pressure recovery on the ethanol at room temperature, and concentrating at 40 ℃ to obtain a thick paste, thus obtaining the callicarpa macrocarpa extract.

the herba Cirsii extract is prepared by the following steps: pulverizing dried herba Cephalanoploris, sieving with 25 mesh sieve, adding 75% ethanol 10 times the weight of herba Cephalanoploris powder, reflux extracting for 3 times (60 min each time), filtering, mixing extractive solutions, standing overnight in ice bath, collecting supernatant, recovering ethanol at 60 deg.C under 45 rpm and 0.08Mpa under reduced pressure, and concentrating at 45 deg.C to obtain herba Cephalanoploris extract.

The campsis grandiflora extract is prepared by the following steps: pulverizing flos Campsis, extracting with distilled water by conventional cold soaking, warm soaking or ultrasonic wave, filtering, collecting filtrate, and spray drying or freeze drying to obtain flos Campsis extract; wherein, the adding amount of the distilled water is 20 times of the weight of the trumpetcreeper pollen, the cold soaking is to soak for 5 days at room temperature, the warm soaking is to soak for 1 hour at 60 ℃, and the extraction times are 2 times; the ultrasonic auxiliary extraction is carried out at 40 ℃, the ultrasonic power is 350W, the ultrasonic frequency is 20KHz, and the ultrasonic output power is 70%, the extraction times are 1 time, and each time lasts for 60 minutes; pre-freezing for 3 hours at the condensation temperature of-30 ℃ in vacuum freeze drying, wherein the vacuum degree is 5 KPa; the air inlet temperature of spray drying is 100 ℃, and the air outlet temperature is 90 ℃.

Example 6

A night cream containing cannabidiol CBD nano-emulsion and mineral substances and a preparation method thereof are disclosed, which comprises the following steps:

(2) Adding a proper amount of water into a moisturizing agent collagen and sodium lactate, fully dissolving at 42 ℃, adding a callicarpa bodinieri extract, a herba cepbalanoplosis segeti extract, stirring to promote dissolution, then adding a thickening agent cetyl alcohol and xanthan gum, fully dissolving, maintaining the temperature at 42 ℃, performing ultrasonic treatment under the conditions that the ultrasonic power is 18%, the ultrasonic starting time is 8s, the ultrasonic closing time is 8s, and the total duration is 12min to obtain a material B;

(3) Mixing grape seed oil serving as a sealing agent, tocopherol serving as an antioxidant and a proper amount of water, heating at 50 ℃ for 12min until the mixture is fully dissolved, cooling at room temperature, adding an emulsifier, namely, sibirak DG-305, fully stirring and uniformly mixing at 40 ℃, dropwise adding a preservative, namely, phenoxyethanol, and fully stirring at the stirring speed of 400r/min to obtain a material C;

(4) adding the material B into the material C, heating at 40 deg.C for 7min, adding CBD nanoemulsion, stirring and mixing for 10min to obtain night cream containing cannabidiol CBD nanoemulsion.

The CBD nanoemulsion is prepared by the following method:

(1) Preparing materials according to the following mass ratio:

(2) Heating oil phase Shea butter, MCT oil and soybean oil at 40 deg.C for 20min, adding cannabidiol, stirring at 40 deg.C until cannabidiol is completely dissolved, and cooling to room temperature to obtain oil solution;

(4) heating and dissolving the emulsifier EL-40, Tween-80, lecithin, poloxamer and triglycerin at 40 ℃ to dissolve, cooling to room temperature, adding glycerol, mixing uniformly, dropwise adding an oil soluble substance, dropwise adding a water soluble substance, stirring while dropwise adding, rotating speed of 500r/min, and obtaining the CBD nanoemulsion when the solution turns turbid from clear to clear.

example 7

A night cream containing nano-emulsion and mineral substances and a preparation method thereof are disclosed, which comprises the following steps:

(2) adding a proper amount of water into a moisturizing agent collagen and sodium lactate, fully dissolving at 50 ℃, adding a callicarpa bodinieri extract, a herba cepbalanoplosis segeti extract and a campsis grandiflora extract, stirring to promote dissolution, adding a thickening agent cetyl alcohol and xanthan gum, fully dissolving, maintaining the temperature at 45 ℃, performing ultrasonic treatment under the conditions that the ultrasonic power is 16%, the ultrasonic starting time is 7s, the ultrasonic closing time is 10s and the total duration is 12min to obtain a material B;

(3) mixing grape seed oil serving as a sealing agent, tocopherol serving as an antioxidant and a proper amount of water, heating at 50 ℃ for 8min until the mixture is fully dissolved, cooling at room temperature, adding an emulsifier, namely, sibirak DG-305, fully stirring and uniformly mixing at 40 ℃, dropwise adding a preservative, namely, phenoxyethanol, and fully stirring at the stirring speed of 350r/min to obtain a material C;

(4) Adding the material B into the material C, heating at 35 deg.C for 10min, adding CBD nanoemulsion, stirring and mixing for 10min to obtain night cream containing cannabidiol CBD nanoemulsion.

The nano-emulsion is prepared by the following method:

(1) Preparing materials according to the following mass ratio:

Examples of the experiments

The invention is tested by the following test examples to test the safety of the product

Experimental example I, examination of microorganisms

The detection method comprises the following steps: refer to technical Specification for safety of cosmetics 2015 year edition

Evaluation indexes are as follows: microbiological examination method according to technical Specification for safety of cosmetics 2015

Detection indexes are as follows:

And (3) detection results:

evaluation criteria

the detection result of the microorganism shows that all indexes of the microorganism accord with the technical standard for cosmetic safety, and the judgment is as follows: the night cream preparation containing cannabidiol CBD nano-emulsion and mineral substances in examples 1-6 and the night cream preparation containing only nano-emulsion and mineral substance in example 7 meet the national standard on the detection results of total bacteria number technology detection and heat-resistant coliform group, staphylococcus aureus, pseudomonas aeruginosa, mould and yeast. The test results of example 7, which does not contain CBD as an active ingredient, are lower in the indexes of bacteria, mold and yeast than those of other examples, indicating that CBD also has bactericidal effect.

Second example of experiment, skin irritation experiment

the experimental method comprises the following steps: refer to technical Specification for safety of cosmetics 2015 edition-skin irritation experiment

Evaluation indexes are as follows: refer to technical Specification for safety of cosmetics 2015 edition-evaluation index of skin irritation experiment

The main reagents are as follows: night cream samples containing cannabidiol CBD nanoemulsion and minerals of examples 1-6, night cream samples containing nanoemulsion and minerals of example 7, normal saline, 75% alcohol

the main materials are as follows: scissors, spreader, dropper;

Experimental animals: white rabbits were kept in a single cage of 6 rabbits (adult, healthy, intact skin, no pregnancy and no farrowing) per example

The specific implementation method comprises the following steps:

Multiple skin irritation experiments

adaptive feeding should be carried out for 3d before the experiment, the hair on two sides of the spine of the experimental animal is cut off about 24h before the experiment, the epidermis cannot be damaged, and the hair removing range is about 3cm multiplied by 3cm respectively on the left and right.

About 0.5mL (g) of the test substance was applied directly to the skin 1 time per day for 14 days. Shearing hairs from the next day before each smearing, removing residual test substance with water or 75% alcohol solvent, observing skin reaction of the smeared part after one hour, and scoring the skin reaction of the tested animals according to the table 1 till the end of 14d experiment.

evaluation indexes are as follows: the average integral per animal per day was calculated according to the following formula, and the skin irritation intensity was determined as shown in Table 2.

results of the experiment

Evaluation results were as follows: in each experimental example, in multiple administration stimulation experiments for 14 consecutive days, an eye observation administration group and a control group are graded and graded according to a response grading standard, and the skin stimulation index of the administration group is less than 0.44; the control group had a skin irritation index of <0.15, met the criteria in table 2, and was a non-irritating product. The test results of example 7, which does not contain CBD as an active ingredient, showed higher skin irritation index than other examples, indicating that CBD also has certain anti-inflammatory, skin-repairing, and skin-soothing effects.

experimental example III statistical evaluation of human body trial experiment

The main reagents are as follows: night cream samples containing cannabidiol CBD nano-emulsion and minerals of examples 1-6, night cream samples containing nano-emulsion and minerals of example 7, and common night cream samples purchased in the market

the subject claims: subject selection according to technical Specification for cosmetic safety 2002

The specific experimental method comprises the following steps:

160 women aged 25-60 are selected, randomly divided into 8 groups, namely an experimental group I, an experimental group II, an experimental group III, an experimental group IV, an experimental group V, an experimental group VI, an experimental group VII and a control group, and the gender and the age degree of the women are statistically processed without significant difference (P >0.05)

② the treatment method comprises: the patients are respectively subjected to trial experience, night cream containing cannabidiol CBD nano-emulsion and mineral substances obtained in examples 1-5 and night cream containing nano-emulsion and mineral substances obtained in example 7 are applied, and the night cream is used for 30 days and is applied 1 time a day after skin is thoroughly cleaned at night; the control group used a commercial night cream product for 30 days, and applied 1 time a day after thoroughly cleaning the skin at night. (wherein experiment group 1 is applied with experiment example 1, experiment group 2 is applied with experiment example 2, and so on)

Evaluation index: comfort, moisturization, whitening effect, smoothness, moisture retention, reduction of skin wrinkles, degree of skin elasticity

Number of effective cases (good), number of effective cases (general), number of ineffective cases (poor/ineffective)

the total effective rate is as follows: (number of effective cases + number of effective cases)/number of experiments

The experimental results are as follows:

evaluation results were as follows: in each experimental example, the satisfaction degree of the samples in the examples 1 to 6 is 95%, 95.7%, 97.14%, 98.54%, 99.29% and 99.29% higher than the satisfaction degree of the control group of 67.14% in a statistical evaluation experiment of 30 consecutive days, wherein the tocopherol (antioxidant) is not added in the example 6, and the investigation result shows that the result is the same as the result in the example 5, and the effect of the tocopherol on the samples is not large.

The satisfaction of the investigation result of the example 7 without cannabidiol CBD active ingredient is lower than that of other examples, which shows that the product prepared by the method of the invention not only can enhance the moisturizing and nourishing effects on the skin, but also can improve the phenomena of darkness, roughness, relaxation, whitening and the like, has certain anti-aging, firming and repairing effects, and the obvious effect can be increased by the persistent application.

Claims

1. night cream containing cannabidiol CBD nano-emulsion is prepared from the following raw materials in percentage by mass:

Saebick DG-3051-10%

Folium Callicarpae Formosanae extract 0.1-1%

herba Cephalanoploris extract 0.1-1%

0.1-1% of campsis grandiflora extract

1 to 15 percent of grape seed oil

0.1 to 0.5 percent of phenoxyethanol

Collagen 0.1-3%

Tocopherol 0.05-1%

1-3% of cetyl alcohol

Xanthan gum 0.1-1%

Sodium lactate 1-5%

CBD nanoemulsion 5-50%

balance of water

the sum of the above components is 100%.

2. Night cream comprising cannabidiol CBD nanoemulsion according to claim 1, characterised in that the CBD nanoemulsion was prepared by the following method:

(1) preparing materials according to the following mass ratio:

cannabidiol CBD 1-15% Tween-801-5%

EL-401-5% lecithin 1-5%

shea butter 1-10% and poloxamer 1-5%

MCT oil 1-10% and triglycerol ester 1-5%

soybean oil 1-5% and mineral concentrated solution 1-20%

1-5% of glycerin with the balance of water

The sum of the components is 100 percent;

3. night cream containing cannabidiol CBD nanoemulsion of claim 1, characterized in that the beautyberry extract is prepared by: crushing the dried callicarpa macrocarpa, sieving with a 20-mesh sieve, adding 75-90% ethanol according to the feed liquid mass ratio of 1:6-10, carrying out ultrasonic extraction for 3 times and 1-3 h/time, filtering, taking filtrate, combining the 3 times of filtrate, carrying out suction filtration after overnight in an ice bath to obtain an extracting solution, carrying out reduced pressure recovery on the ethanol at room temperature, and concentrating at 40-50 ℃ to obtain a thick paste to obtain the callicarpa macrocarpa extract.

4. The night cream comprising cannabidiol CBD nanoemulsion of claim 1, wherein the extract of herba Cirsii is obtained by: crushing dried common cephalanoplos herb, sieving the powder by a sieve of 25-40 meshes, adding 75% ethanol by mass according to 8-15 times of the mass of the common cephalanoplos herb powder, performing reflux extraction for 1-3 times, each time for 30-120 minutes, filtering, combining extracting solutions, performing overnight cooling in an ice bath, taking supernate, recovering ethanol under reduced pressure at the conditions of 30-60 ℃, rotation speed of 30-60 r/min and 0.06-0.08 Mpa, and concentrating the supernate to be thick paste at 45 ℃ to obtain the common cephalanoplos herb extract.

5. The night cream containing cannabidiol CBD nanoemulsion of claim 1, wherein the trumpet creeper extract is prepared by: pulverizing flos Campsis, extracting with distilled water by conventional cold soaking, warm soaking, and ultrasonic wave, filtering, collecting filtrate, and spray drying or freeze drying to obtain flos Campsis extract; wherein the adding amount of the distilled water is 10-50 times of the mass of the trumpetcreeper pollen, the cold soaking is carried out for 1-10 days at room temperature, the warm soaking is carried out for 1-3 hours at 40-60 ℃, and the extraction times are 1-3 times; the ultrasonic-assisted extraction is carried out at the temperature of 40-60 ℃, the ultrasonic power of 100-650W, the ultrasonic frequency of 19-23 KHz and the ultrasonic output power of 70-100%, the extraction times are 1-3, and each time lasts for 30-60 minutes; pre-freezing for 3-16 hours at the condensation temperature of-20 to-50 ℃ in vacuum freeze drying, wherein the vacuum degree is 5-20 KPa; the air inlet temperature of spray drying is 100-300 ℃, and the air outlet temperature is 30-140 ℃.

6. the night cream containing cannabidiol CBD nanoemulsion of claim 1, prepared by the following steps:

1) Preparing materials according to the following mass ratio:

Saebick DG-3051-10%

Folium Callicarpae Formosanae extract 0.1-1%

Herba Cephalanoploris extract 0.1-1%

0.1-1% of campsis grandiflora extract

1 to 15 percent of grape seed oil

0.1 to 0.5 percent of phenoxyethanol

collagen 0.1-3%

Tocopherol 0.05-1%

1-3% of cetyl alcohol

xanthan gum 0.1-1%

Sodium lactate 1-5%

CBD nanoemulsion 5-50%

Balance of water

The sum of the components is 100 percent;

2) Adding a proper amount of water into a moisturizing agent collagen and sodium lactate, fully dissolving at 40-60 ℃, adding a callicarpa bodinieri extract, a herba cepbalanoplosis segeti extract, stirring to promote dissolution, adding a thickening agent cetyl alcohol and xanthan gum, fully dissolving, maintaining the temperature at 35-45 ℃, performing ultrasonic treatment under the conditions that the ultrasonic power is 10-20%, the ultrasonic starting time is 5-10 s, the ultrasonic closing time is 5-10 s, and the total duration is 5-15 min to obtain a material B;

3) mixing grape seed oil serving as a sealing agent, tocopherol serving as an antioxidant and a proper amount of water, heating at 40-60 ℃ for 5-15 min until the mixture is fully dissolved, cooling at room temperature, adding an emulsifier, namely, sibirak DG-305, fully stirring and uniformly mixing at 30-40 ℃, dropwise adding a preservative, namely, phenoxyethanol, and fully stirring at the stirring speed of 300-500 r/min to obtain a material C;

4) and adding the material B into the material C, heating at 30-40 ℃ for 5-10 min, adding the CBD nanoemulsion, and fully stirring and mixing for 5-15 min to obtain the night cream containing the cannabidiol CBD nanoemulsion.