CN111440896A - 一种新型β冠状病毒变异检测方法、探针和试剂盒 - Google Patents
一种新型β冠状病毒变异检测方法、探针和试剂盒 Download PDFInfo
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Abstract
一种新型β冠状病毒(SARS‑CoV‑2)变异检测试剂盒，其特征在于，包括以下部分：RNA探针组，引物，封闭缓冲液，RNA酶封闭液，杂交缓冲液，结合缓冲液，1倍浓度的SSC的漂洗液，0.1倍浓度的SSC的漂洗液，PCR反应液和TE缓冲液；所述的RNA探针组包括311条RNA探针，所述的RNA探针序列如SEQ ID NO.1～SEQ ID NO.311。本发明提供的一种用于新型β冠状病毒(SARS‑CoV‑2)变异检测试剂盒，可以对文库中的β冠状病毒核酸序列进行富集，提高检测灵敏性，同时检测所有人和动物的β冠状病毒，用于β冠状病毒的检测和遗传变异检测。
Description
技术领域
本发明涉及生物基因检测领域，尤其涉及一种基于液相探针杂交捕获的新型β冠状病毒(SARS-CoV-2)变异检测方法、探针和试剂盒。
背景技术
SARS-CoV-2与SARS冠状病毒和“中东呼吸综合征”MERS冠状病毒，同属于β属冠状病毒，基因组为线性正链RNA。作为RNA病毒，新型冠状病毒具有很高的变异频率，其在野生动物-中间宿主-人类之间传播的过程中会发生较多变异。基于实时荧光定量PCR (RT-qPCR)方法的新型冠状病毒核酸检测试剂盒，只针对新型冠状病毒最初发布的特异保守序列区域或某一病株抗原进行定性检测。该方法对基因变异容忍性低。如果病毒基因变异发生在引物和探针特异结合区域，会造成漏检形成假阴性诊断；同时这种方法仅限定于判断所检特定病毒的有无，无法判断病毒在各物种或人际传播过程中所发生的适应性突变而产生的新种病毒。另外，国家药监局快速批复了一款基于宏基因组建库结合二代测序技术(mNGS)的体外诊断产品。然而该方法所需的测序数据量巨大，测序成本高昂；并且该方法针对隐匿期病人样本或病毒丰度较低的样本，所需数据量并无上限，所得测序结果较难拼接成得到全长序列，较难用于病毒基因序列变异的检测。
因此，本领域需要一种通用冠状病毒属基因组检测方法，以较低成本对SARS-CoV-2 进行全面精准检测，并对动物宿主和病毒携带者进行监控和检测。
发明内容
鉴于现有技术中存在的问题，本发明的目的在于提供一种基于液相探针杂交捕获技术开发的新型β冠状病毒变异检测方法、探针和试剂盒，可以以较低成本对SARS-CoV-2进行全面精准检测，以便于对动物宿主和病毒携带者进行监控和检测，监控病毒的变异和传播路径。
在第一方面，本发明提供了一种新型β冠状病毒(SARS-CoV-2)变异检测序列组合，所述序列组合包括RNA探针组或其对应的DNA探针组，所述的RNA探针组包括311 条RNA探针，所述的RNA探针序列如SEQ ID NO.1～SEQ ID NO.311所示。
在一个实施方案中，所述序列组合还包括接头序列、第一引物组和第二引物组。所述接头序列包括SEQ ID NO.334和SEQ ID NO.335。所述第一引物组选自序列为SEQ IDNO.314～SEQ ID NO.333的引物对，用于文库扩增。其中，SEQ ID NO.314和324、SEQ IDNO.315和325、SEQ ID NO.316和326、SEQ ID NO.317和327、SEQ ID NO.318和328、 SEQ IDNO.319和329、SEQ ID NO.320和330、SEQ ID NO.321和331、SEQ ID NO.322 和332、SEQ IDNO.323和333配对使用。所述第二引物组包括正向引物和反向引物，用于杂交捕获文库序列的扩增。优选地，所述正向引物序列为SEQ ID NO.312，所述反向引物序列为SEQ IDNO.313。
在第二方面，本发明提供了一种新型β冠状病毒(SARS-CoV-2)变异检测试剂盒，所述试剂盒包括RNA探针组或其对应的DNA探针组，所述的RNA探针组包括311条 RNA探针，所述的RNA探针序列如SEQ ID NO.1～SEQ ID NO.311所示。
在一个实施方案中，所述的试剂盒还包括：第一引物对、封闭缓冲液、RNA酶封闭液、杂交缓冲液、结合缓冲液、1倍浓度的SSC漂洗液、0.1倍浓度的SSC漂洗液、PCR 反应液和TE缓冲液。
在一个实施方案中，所述试剂盒还包括接头序列、第一引物组和第二引物组。所述接头序列包括SEQ ID NO.334和SEQ ID NO.335，用于连接文库序列两端。所述第一引物组选自序列为SEQ ID NO.314～SEQ ID NO.333的引物对，用于文库扩增。其中，SEQ IDNO.314和324、SEQ ID NO.315和325、SEQ ID NO.316和326、SEQ ID NO.317和327、 SEQ IDNO.318和328、SEQ ID NO.319和329、SEQ ID NO.320和330、SEQ ID NO.321 和331、SEQ IDNO.322和332、SEQ ID NO.323和333配对使用。所述第二引物组包括正向引物和反向引物，用于杂交捕获文库序列的扩增。优选地，所述正向引物序列为SEQ ID NO.312，所述反向引物序列为SEQ ID NO.313。
在一个实施方案中，所述的RNA探针为生物素标记的RNA探针。
在一个实施方案中，所述的封闭缓冲液包括人cot-1DNA、鲑鱼精DNA和通用型封闭序列。
在一个实施方案中，所述的杂交缓冲液包括SSPE溶液、Denhardt溶液、EDTA、SDS和甲酰胺。
在一个实施方案中，所述的结合缓冲液包括NaCl、Tris-HCl和EDTA。
在一个实施方案中，所述RNA酶封闭液为RNA酶抑制剂。
在一个实施方案中，所述1倍浓度的SSC漂洗液包括1倍浓度的SSC溶液和0.1％SDS；
所述0.1倍浓度的SSC漂洗液包括0.1倍浓度的SSC溶液、0.1％SDS和甲酰胺。
在一个实施方案中，所述PCR反应液包括dNTPs、Taq DNA聚合酶、和PCR反应缓冲液。
在一个实施方案中，所述TE缓冲液包括10mM Tris-HCl(pH 8.0)和1mM EDTA(pH8.0)。
在第三方面，本发明提供了一种新型β冠状病毒(SARS-CoV-2)变异检测方法，所述方法包括：
(1)提取病毒样本的RNA；
(2)构建总RNA文库，总RNA文库包括由RNA逆转录而成的cDNA双链序列和接头；
(3)对所述RNA文库用RNA探针组进行两轮捕获和捕获后扩增，所述的RNA探针组包括311条RNA探针或其对应的DNA探针，所述的RNA探针序列如SEQ ID NO.1～SEQ ID NO.311所示；
(4)对所述两轮捕获后扩增的扩增物进行测序。
在一个实施方案中，所述方法还包括：对所述测序结果进行分析。
在一个实施方案中，所述分析包括：
(1)将测序数据定位到SARS-CoV-2的基因组数据MN908947.3的相应位置上，同时进行各项指标统计，包括比对率、覆盖率、重复率、捕获率、平均深度、均一性指标；
(2)将测序数据比对人类参考基因组，同时分离出比对不上人类参考基因组的结果；
(3)将上一步分离出的结果比对病毒库，选取比对到病毒库的结果，利用Trinity软件进行组装，得到病毒的组装结果；
(4)将组装结果比对病毒库，确定病毒的分型结果是否为MN908947.3；
(5)选取(4)比对到MN908947.3基因组上的结果，进行突变寻找，突变包括SNP 和InDel两种类型，确定突变位点发生的基因、位置、功能、氨基酸改变等。
在第四方面，本发明提供了RNA探针组用于制备检测新型β冠状病毒(SARS-CoV-2)变异的制剂的用途，所述的RNA探针组包括311条RNA探针或其对应的DNA探针，所述的RNA探针序列如SEQ ID NO.1～SEQ ID NO.311所示；所述检测新型β冠状病毒 (SARS-CoV-2)变异通过本发明第三方面的方法进行。
本发明提供的一种用于新型β冠状病毒(SARS-CoV-2)变异检测方法、探针和试剂盒，可以对文库中的β冠状病毒核酸序列进行富集，提高检测灵敏性，同时检测所有人和动物的β冠状病毒，用于β冠状病毒的检测和遗传变异检测。
附图说明
图1示出了生物信息分析流程图。
具体实施方式
需要说明的是，所述的RNA探针组包括311条RNA探针，这些探针可以通过相应的DNA探针转录而来，因此311条RNA探针或者相应的311条DNA探针均在本发明的范围内。提及RNA探针或DNA探针，亦包括对应的DNA探针或RNA探针形式。
实施例
1、材料与方法
1.1试剂
1)病毒RNA提取试剂盒，购自天根生化(北京)科技有限公司，DP315-R；
2)RNA文库构建试剂盒，购自ABclonal，RK20301；
3)探针杂交捕获试剂盒使用艾吉泰康(北京)生物科技有限公司TargetSeq Onekit。
1.2方法
1.2.1探针设计
基于已公布的新型冠状病毒全基因组序列，以及NCBI数据库中已有的相关冠状病毒属序列，设计并合成液相探针检测试剂盒，覆盖目前已知所有的β冠状病毒全基因组序列。用于设计探针的病毒基因组如下：1)SARS-CoV-2：包含32条序列(来源：武汉18条，广州6个，浙江2条，美国2条，泰国暖武里府2条，日本神奈川1条，以及蝙蝠RaTG13)。2)其他β冠状病毒属毒株：共计1122条。涵盖了SARS、MERS、牛冠状病毒、鼠冠状病毒、马冠状病毒、猪冠状病毒、犬冠状病毒。
然后，发明人将所述探针在实验过程中进行逐步筛选和优化，实现以最经济的序列达到最大覆盖，并且考虑病毒的变异快等特点，对RNA探针进行优化和取舍选择。最终选出的RNA探针组包括311条RNA探针，所述的RNA探针序列如SEQ ID NO.1 ～SEQ ID NO.311，所述的RNA探针组对SARS-CoV-2病毒的覆盖度为100％，整体覆盖度为99.7441％。
1.2.2病毒总RNA提取：
参照病毒RNA提取试剂盒(TIANGEN，Cat No:DP315-R)使用说明提取病毒总 RNA。
1.2.3-1.2.9步骤参照RNA逆转录及建库试剂盒说明书进行：也可使用如下试剂盒构建RNA文库：诺唯赞VAHTS Stranded mRNA-seq Library Prep Kit；诺唯赞TruePrep RNALibrary Prep Kit；NEBNext Ultra II文库制备试剂盒；NEBNext Ultra II定向文库制备试剂盒。
1.2.3总RNA打断
按照表1配制RNA打断体系：
表1总RNA打断体系
| 试剂 | 体积(μL) |
| 2×Frag/Elute buffer | 6 |
| 总RNA | 6 |
| 总体积 | 16 |
吹打混匀后，置于94℃，10min打断成300-450nt的片段
1.2.4 cDNA第一链合成
1)取出RT Strand Specificity Reagent室温溶解，冰上配制如下体系：
表2 cDNA第一链合成体系
| 试剂 | 体积(μL) |
| 打断后mRNA | 10 |
| RT Strand Specificity Reagent | 8 |
| First Strand synthesis enzyme mix | 2 |
| 总体积 | 20 |
2)使用移液器吹打混匀，瞬时离心，将体系置于PCR仪：
| 温度 | 时间 |
| 25℃ | 10min |
| 42℃ | 15min |
| 70℃ | 15min |
| 4℃ | 保持 |
1.2.5 cDNA第二链合成
1)将Second strand synthesis reaction buffer从冰箱中取出冰上溶解，并按照表3依次加入各试剂：
表3 cDNA第二链合成配制体系
| 试剂 | 体积 |
| First strand cDNA | 20μL |
| Second strand synthesis reaction buffer with dUTP | 8μL |
| Second strand synthesis enzyme mix | 4μL |
| Nuclease-free水 | 48μL |
| 总体积 | 80μL |
2)使用移液器吹打混匀，瞬时离心后，将样本置于PCR仪(热盖温度关闭)，16℃孵育1h；
3)提前将Agencourt AMPure XP beads从2-8℃取出，静置平衡至室温，使用前涡旋或者震荡混匀；
4)样本孵育结束后，每个样本加入144μL Agencourt AMPure XP beads(1.8×)，枪头吹打混匀；
5)室温静置5min，然后转移至磁力架上～5min，直至溶液变澄清，小心弃除上清；
6)将离心管保持在磁力架上，加入200μL 80％乙醇，静置30s，弃除全部上清；
7)重复3.6，将磁珠用80％乙醇再洗1次后，用10μL枪头将残留液体彻底吸干；
8)干燥磁珠2-3min，待酒精挥发完全后，加入39μL Low EDTA TE，吹打混匀；
9)室温静置2min，磁力架上1min，直到溶液变澄清，小心吸取37μL上清至另一离心管中；
1.2.6末端修复
1)将End-prep buffer从冰箱中取出，冰上溶解后配制如下体系：
表4末端修复配制反应体系
| 试剂 | 体积 |
| 双链cDNA | 37μL |
| End-prep buffer | 10μL |
| End-prep enzyme mix | 3μL |
| 总体积 | 50μL |
2)枪头吹打混匀，瞬时离心，PCR仪上进行如下孵育：
| 温度 | 时间 |
| 20℃ | 30min |
| 65℃ | 30min |
| 4℃ | 保持 |
1.2.7接头连接
1)将Ligation Buffer，Insert UMI Adapter(SEQ ID NO.334和SEQ ID NO.335)取出冰上溶解，在冰上配制接头连接体系：
表5接头连接体系
| 试剂 | 体积 |
| End-prep DNA | 50μL |
| Ligation Buffer | 16.5μL |
| Insert UMI Adapter for Illumina* | 2.5μL |
| Ligase mix | 3μL |
| 总体积 | 70μL |
2)吹打混匀，瞬时离心，PCR仪上进行连接反应(热盖温度关闭)：
| 温度 | 时间 |
| 22℃ | 15min |
1.2.8连接产物纯化
1)提前将Agencourt AMPure XP beads从2-8℃取出，静置平衡至室温，使用前涡旋或者震荡混匀；
2)连接反应结束后，在产物中加入56μL Agencourt AMPure XP beads(0.8×)，吹打混匀；
3)室温静置5min，然后转移至磁力架上～5min，直至溶液变澄清，小心弃除上清；
4)将离心管保持在磁力架上，加入200μL 80％乙醇，静置30s，弃除全部上清；
5)重复6.4，将磁珠用80％乙醇再洗1次，用10μL枪头将残留液体彻底吸干；
6)干燥磁珠2-3min，待酒精挥发完全后，加入21μL Low EDTA TE，吹打混匀；
7)室温静置2min，磁力架上1min，直到溶液变澄清，小心吸取19.5μL上清至另一新的PCR管中备用；
1.2.9 PCR扩增文库
1)将连接纯化后产物，按照下表配制PCR体系：
表6 PCR扩增文库反应体系
SEQ ID NO.314和324、SEQ ID NO.315和325、SEQ ID NO.316和326、SEQ IDNO.317和327、SEQ ID NO.318和328、SEQ ID NO.319和329、SEQ ID NO.320和 330、SEQ IDNO.321和331、SEQ ID NO.322和332、SEQ ID NO.323和333配对使用，用于扩增文库，每组引物用于一个样本，以区分样本。
2)枪头吹打混匀，微离心，PCR仪中按照如下程序进行PCR:
3)提前将Agencourt AMPure XP beads从2-8℃取出，静置平衡至室温，使用前涡旋或者震荡混匀；
4)反应结束后，每个反应管中加入50μL Agencourt AMPure XP beads(1×)，吹打混匀；
5)室温静置5min，然后转移至磁力架上～5min，直至溶液变澄清，小心弃除上清；
6)将离心管保持在磁力架上，加入200μL 80％乙醇，静置30s，弃除全部上清；
7)重复6)，将磁珠用80％乙醇再洗1次，用10μL枪头将残留液体彻底吸干；
8)干燥磁珠2-3min，待酒精挥发完全后，加入31μL Low EDTA TE，吹打混匀；
9)室温静置2min，磁力架上1min，直到溶液变澄清，小心吸取30μL文库至另一新的离心管中，留存备用。
1.2.10-1.2.14参考艾吉泰康(北京)生物科技有限公司TargetSeq One kit说明书进行：
1.2.10文库准备和浓缩
1)预先从﹣20℃保存的捕获试剂盒中，取出下表所示Block试剂，放置于冰盒上溶解，溶解混匀后置于冰上或4℃待用；
2)将样品文库与表7中Block反应体系配制，混匀后标记为B管表7 Block体系(通用Block)
Universal Block推荐使用iGeneTech Universal Blocking Oligo Kit(货号TBLU-i96)。
3)将B管放入真空浓缩离心机，打开PCR管盖，启动离心机，打开真空泵开关，开始浓缩(浓缩之前可以先用相同体积的水进行浓缩时间测试，计算样品单位体积减少的时间，确认浓缩速率，避免因浓缩时间过长导致过度干燥，造成样品损耗)；
4)将B管浓缩至体积小于10μl，之后用Nuclease-free水补足至10μl，轻轻吸打混匀，短暂离心后置于冰上待用。
1.2.11探针杂交
1)将
One Hyb Buffer置于室温融化，混匀后置于50℃恒温混匀仪内预热，完全溶解后(无沉淀及浑浊物)每个反应取20μl
One Hyb Buffer置于新的200μl PCR管内，盖好管盖，置于50℃恒温混匀仪孵育待用，标记为A管；
2)取5μl RNase Block与2μl Probe置于200μl PCR管内，轻轻吸打混匀，短暂离心后置于冰上或4℃待用，标记为C管；
3)从A管吸取13μl与B管10μl加入C管中震荡混匀，短暂离心后置于冰上待用；
4)设置PCR仪参数如下：
Heat lid 85℃
80℃维持5min
50℃保持
5)将PCR管C置于PCR仪上，运行以上程序，孵育2h。
1.2.12捕获磁珠平衡
1)捕获磁珠(Cap beads)从4℃取出，涡旋震荡重悬，置于室温平衡30min；
2)取50μl磁珠置于新的PCR管内，置于磁力架上1min使溶液澄清，移除上清；
3)从磁力架上取下PCR管，加入200μl Binding Buffer轻轻吸打数次混匀，重悬磁珠；
4)置磁力架上1min，移除上清；
5)重复步骤3)-4)两次，共清洗磁珠3次；
6)从磁力架上取下PCR管，加入180μl Binding Buffer轻轻吸打6次重悬磁珠待用。
1.2.13捕获目标区域DNA文库
1)保持杂交产物在PCR仪上，将步骤1.2.12中6)重悬后的180μl Cap beads 加入到杂交产物中，用移液器吸打6次混匀，置于旋转混匀仪上室温结合30min；
2)将PCR管置于磁力架上2min使溶液澄清，移除上清液；
3)向杂交产物内加入200μl的Wash Buffer 1，轻轻吸打6次混匀，置于旋转混匀仪上清洗15min，然后短暂离心，将PCR管放于磁力架上2min，使溶液澄清，移除上清；
4)加入200μl的50℃预热的
One Wash Buffer 2，轻轻吸打6次混匀，置于恒温振荡混匀仪上50℃孵育10min；
5)短暂离心，将PCR管放于磁力架上2min，移除上清。使用
One WashBuffer 2清洗2次，共计3次。最后一次彻底移除
One Wash Buffer 2(可用10μl移液器移除残留)；
6)保持样品在磁力架上，向PCR管内加入200μl 80％乙醇，静置30s后彻底移除乙醇溶液(可用10μl移液器移除残留)，室温晾干；
7)向PCR管加入30μl Nuclease-free water，从磁力架上取下PCR管，轻轻吸打6次重悬磁珠待用。
1.2.14 Post-PCR反应
1.2.14.1实验前准备
1)预先从-20℃保存的试剂盒中取出Post PCR Buffer、Post PCR Primer(25μM，for ILM)试剂，放置冰上溶解，溶解混匀后置于冰上待用；取出Post PCR Polymerase 简短离心后立即使用。
2)捕获后需要对DNA文库进行富集，根据下表配制反应体系：
表8 Post-PCR体系
3)将移液器调至40μl，轻轻吸打混匀6次，然后立即置于PCR仪上。
1.2.14.2 Post-PCR实验
1)运行PCR仪程序：
| Heat lid | 105℃ |
| 95℃ | 持续1min |
N个循环(N参考探针Probe管壁标签参数或探针说明文件)
| 98℃ | 持续20s |
| 60℃ | 持续30s |
| 72℃ | 持续30s |
| 72℃ | 5min |
| 4℃ | 保持 |
2)PCR结束后向样品加入55μl Agencourt AMPure XP磁珠，用移液器轻轻吸打 6次混匀；
3)室温孵育5min，把PCR管置于磁力架上3min使溶液澄清；
4)移除上清，PCR管继续置于磁力架上，加入200μl 80％乙醇，静置30s；
5)移除上清，再向PCR管内加入200μl 80％乙醇，静置30s后彻底移除上清(可用10μl移液器移除底部残留酒精)；
6)室温放置5min，使得残留乙醇彻底挥发；
7)加入25μl Nuclease-free water，将PCR管从磁力架拿下，轻轻吹打混匀重悬磁珠，室温放置2min；
8)将PCR管置于磁力架上2min；
9)用移液器吸23μl上清液转移到1.5ml离心管，标记样品信息；
10)取1μl文库使用Qubit dsDNA HS Assay Kit进行定量，记录文库浓度，文库浓度约在1-20ng/μl；
11)取1μl样品使用Agilent 2100Bioanalyzer system(Agilent DNA 1000Kit)进行片段长度测定，文库长度约在270bp-320bp间；
12)得到文库使用Illumina NovaSeq6000高通量测序平台进行测序。
1.2.15二次捕获
重复1.2.10-1.2.14步骤进行二次捕获，得到文库使用Illumina NovaSeq6000高通量测序平台进行测序。
1.2.16生物信息分析
生物信息学分析包括原始数据质控、参考基因组比对、组装、突变寻找等步骤，主要产出结果为：指标统计结果、病毒基因组组装结果、分型结果、突变结果。详细步骤如图1所示。
图1示出了生物信息分析流程图。简单描述如下：i.获得原始下机数据以后，用Trimmomatic软件对原始数据进行质控，去除测序数据中的接头和低质量数据；ii.把过滤后的结果用bwa软件定位到SARS-CoV-2的基因组数据MN908947.3(从NCBI数据库中下载：https://www.ncbi.nlm.nih.gov/nuccore/MN908947.3/)的相应位置上，同时进行各项指标统计，包括比对率、覆盖率、重复率、捕获率、平均深度、均一性指标；iii.将过滤后的结果用bowtie2软件比对人类参考基因组hg19(GRCh37/hg19，来自http://hgdownload.soe.ucsc.edu/downloads.html)，同时分离出比对不上hg19的结果；iv.将上一步分离出的结果比对病毒库(新冠病毒基因组数据，例如MN908947.3)，选取比对到病毒库的结果，利用Trinity软件进行组装，得到病毒的组装结果；v.将组装结果用blast软件比对病毒库(新冠病毒基因组数据，例如MN908947.3)，确定病毒的分型结果是否为MN908947.3；vi.选取第v步比对到MN908947.3基因组上的结果，利用GATK软件进行突变寻找，突变包括SNP(单核苷酸多态性突变)和InDel (插入突变和缺失突变)两种类型，同时利用SnpEff软件对找出的突变结果进行注释，确定突变位点发生的基因、位置、功能、氨基酸改变等。
1.3本发明的有效性及相较于RT-qPCR的优势
为验证本发明检测新冠病毒的有效性及相较RT-qPCR而有的检测变异优势，我们设计如下试验进行检测比较。使用人总RNA稀释新冠病毒培养物中提取的总RNA，稀释0倍、100和1000倍。稀释不同比例的总RNA构建二次捕获文库上机测序。人总RNA构建的相应文库作为阴性对照。同时，利用RT-qPCR(新冠病毒特异性探针) 方法检测稀释0倍、100倍和1000倍样品。结果见表9，总结如下：
1)对于稀释0倍，100倍，1000倍样品和阴性对照样品，使用本发明和RT-qPCR 方法分别进行检测，Ct值分别为28，33和40，鉴定为新冠病毒阳性样本，并与样品稀释梯度趋势一致。本发明检测结果也为新冠病毒阳性样本，平均覆盖深度趋势也表现出很好的一致性。阴性对照样品，两种方法均鉴定为阴性样本。
2)RT-qPCR仅能定性鉴定新冠病毒的有无，并不能检测变异；并且如果病毒RNA 变异发生在探针和引物区域，会造成假阴性诊断。本发明设计探针区域覆盖≥99％的新冠病毒基因组区域，不仅可以定性鉴定新冠病毒的有无，而且可以检测病毒变异 (表10)，追溯其可能来源。
表9本发明与RT-qPCR检测新冠病毒基因数据
表10本发明鉴定的新冠病毒变异种类和数量
1.4本发明相较于mNGS方法的优势
为阐明本发明相较于mNGS方法的较低检测限和节省成本优势，我们设计如下试验作了对比。使用人总RNA稀释新冠病毒培养物中提取的总RNA，稀释102～108倍。稀释不同比例的总RNA构建宏基因组文库、一次捕获文库和二次捕获文库，分别上机测序。人总RNA构建的相应文库作为阴性对照。结果见表11，总结如下：
1)3种阴性对照文库(人总RNA文库)均没有检测到病毒基因目标区域的序列，表明阴性对照文库合格。
2)mNGS方法(～1G干净测序数据)检测稀释100倍的新冠病毒样品，，目标区域覆盖度仅为1.7％，平均覆盖深度为0.03。此测序深度不足以拼接病毒基因全长，无法鉴定新冠病毒变异。对于稀释倍数≥1000倍的RNA样品，均没有检测到病毒基因序列。因此，mNGS方法检测病毒丰度≤0.1％的样本，需要更大数据量才可能检测到病毒序列，而且检测到的序列不足以拼接成病毒基因序列。若需拼接病毒基因全长，数据量大到不可估量，成本高昂。
3)本发明提供的新冠病毒检测方法，病毒基因富集效果明显。一次捕获技术对于病毒RNA丰度为10-6的样品的检测，在相同数据量情况下，可与mNGS方法检测病毒RNA丰度为10-2的样品的效果相同。病毒RNA富集效果提高了10000倍；二次捕获技术富集效果更加明显，富集效果在一次捕获文库基础上又提高了100倍。
4)本发明的二次捕获文库对于病毒丰度≥10-4的样本，～1G数据量可覆盖95％以上病毒基因组序列，可检测病毒变异，追溯病毒来源。
5)总之，本发明与mNGS方法相比，具有更高检测限，所需成本更低。
表11本发明与mNGS方法检测新冠病毒基因数据
1.5 SARS-CoV-2感染患者检测案例
对8例经过RT-qPCR确诊的患者咽拭子总RNA进行建库和冠状病毒目标区域捕获测序，冠状病毒属基因目标区域捕获测序数据质量如表12和表13所示，91％以上的原始短序列可准确比对回参考序列，说明病毒目标基因富集显著；目标区域的平均有效测序数据量达到3.52Mb，均鉴定为新冠病毒阳性样本，并可检测变异，追溯其可能来源。
表12新型冠状病毒属基因目标区域捕获测序数据质量
表13新型冠状病毒基因变异数目
2结论
综上所述，以上8例新型冠状病毒感染者，均检测到SARS-CoV-2基因及其变异。本发明提供的用于检测新型冠状病毒变异的探针组及试剂盒，相比RT-qPCR检测方法，具有全面准确，特异性高等特点。与mNGS相比，本发明提供的探针组和试剂盒在相同数据量情况下，具有更高灵敏性，成本更低。总之，本发明可作为一种新的新冠病毒检测替代方案，用于鉴定新型冠状病毒及其变异，通过进一步分析可检测病毒进化，追溯病毒来源，为实现宿主追踪和病原防控提供理论基础和指导。
序列如下：(其中N表示任意ACTG)
SEQ ID NO.1:aucuaagucacuaacugagaauaaauauucucaauuagaugaagagcaaccaauggagau ugauuaaacgaacaugaaaauuauucuuuucuuggcacug
SEQ ID NO.2:gauaacacucgcuacuugugagcuuuaucacuaccaagaguguguuagagguacaacagu acuuuuaaaagaaccuugcucuucuggaacauacgagggc
SEQ ID NO.3:gggcaauucaccauuucauccucuagcugauaacaaauuugcacugacuugcuuuagcac ucaauuugcuuuugcuuguccugacggcguaaaacacguc
SEQ ID NO.4:ucaguuacgugccagaucaguuucaccuaaacuguucaucagacaagaggaaguucaaga acuuuacucuccaauuuuucuuauuguugcggcaauagug
SEQ ID NO.5:guuuauaacacuuugcuucacacucaaaagaaagacagaaugauugaacuuucauuaauu gacuucuauuugugcuuuuuagccuuucugcuauuccuug
SEQ ID NO.6:guuuuaauuaugcuuauuaucuuuugguucucacuugaacugcaagaucauaaugaaac uugucacgccuaaacgaacaugaaauuucuuguuuucuuag
SEQ ID NO.7:ggaaucaucacaacuguagcugcauuucaccaagaauguaguuuacagucauguacucaa caucaaccauauguaguugaugacccguguccuauucacu
SEQ ID NO.8:cuucuauucuaaaugguauauuagaguaggagcuagaaaaucagcaccuuuaauugaauu gugcguggaugaggcugguucuaaaucacccauucaguac
SEQ ID NO.9:aucgauaucgguaauuauacaguuuccuguuuaccuuuuacaauuaauugccaggaaccu aaauuggguagucuuguagugcguuguucguucuaugaag
SEQ ID NO.10:gacuuuuuagaguaucaugacguucguguuguuuuagauuucaucuaaacgaacaaac uaaaaugucugauaauggaccccaaaaucagcgaaaugcacc
SEQ ID NO.11:cccgcauuacguuugguggacccucagauucaacuggcaguaaccagaauggagaacgc aguggggcgcgaucaaaacaacgucggccccaagguuuacc
SEQ ID NO.12:ccaauaauacugcgucuugguucaccgcucucacucaacauggcaaggaagaccuuaaa uucccucgaggacaaggcguuccaauuaacaccaauagcag
SEQ ID NO.13:guccagaugaccaaauuggcuacuaccgaagagcuaccagacgaauucgugguggugac gguaaaaugaaagaucucaguccaagaugguauuucuacua
SEQ ID NO.14:ccuaggaacugggccagaagcuggacuucccuauggugcuaacaaagacggcaucauau ggguugcaacugagggagccuugaauacaccaaaagaucac
SEQ ID NO.15:acauuggcacccgcaauccugcuaacaaugcugcaaucgugcuacaacuuccucaagga acaacauugccaaaaggcuucuacgcagaagggagcagagg
SEQ ID NO.16:cggcagucaagccucuucucguuccucaucacguagucgcaacaguucaagaaauucaa cuccaggcagcaguaggggaacuucuccugcuagaauggcu
SEQ ID NO.17:cuggcaauggcggugaugcugcucuugcuuugcugcugcuugacagauugaaccagcu ugagagcaaaaugucugguaaaggccaacaacaacaaggcca
SEQ ID NO.18:aaacugucacuaagaaaucugcugcugaggcuucuaagaagccucggcaaaaacguacu gccacuaaagcauacaauguaacacaagcuuucggcagacg
SEQ ID NO.19:gugguccagaacaaacccaaggaaauuuuggggaccaggaacuaaucagacaaggaacu gauuacaaacauuggccgcaaauugcacaauuugcccccag
SEQ ID NO.20:cgcuucagcguucuucggaaugucgcgcauuggcauggaagucacaccuucgggaacg ugguugaccuacacaggugccaucaaauuggaugacaaagau
SEQ ID NO.21:auccaaauuucaaagaucaagucauuuugcugaauaagcauauugacgcauacaaaaca uucccaccaacagagccuaaaaaggacaaaaagaagaaggc
SEQ ID NO.22:gcugaugaaacucaagccuuaccgcagagacagaagaaacagcaaacugugacucuucu uccugcugcagauuuggaugauuucuccaaacaauugcaac
SEQ ID NO.23:caauccaugagcagugcugacucaacucaggccuaaacucaugcagaccacacaaggcag augggcuauauaaacguuuucgcuuuuccguuuacgauau
SEQ ID NO.24:auagucuacucuugugcagaaugaauucucguaacuacauagcacaaguagauguaguu aacuuuaaucucacauagcaaucuuuaaucaguguguaaca
SEQ ID NO.25:auuagggaggacuugaaagagccaccacauuuucaccgaggccacgcggaguacgaucg aguguacagugaacaaugcuagggagagcugccuauaugga
SEQ ID NO.26:uauggaagagcccuaauguguaaaauuaauuuuaguagugcuauccccaugugauuuu aauagcuucuuaggagaaugacaaaaaaaaaaaaaaaaaaaa
SEQ ID NO.27:cucuaaagaggguuuuuucacuuacauuuguggguuuauacaacaaaagcuagcucuu ggagguuccguggcuauaaagauaacagaacauucuuggaau
SEQ ID NO.28:ugcugaucuuuauaagcucaugggacacuucgcaugguggacagccuuuguuacuaau gugaaugcgucaucaucugaagcauuuuuaauuggauguaau
SEQ ID NO.29:uuaucuuggcaaaccacgcgaacaaauagaugguuaugucaugcaugcaaauuacauau uuuggaggaauacaaauccaauucaguugucuuccuauucu
SEQ ID NO.30:cuuuauuugacaugaguaaauuuccccuuaaauuaagggguacugcuguuaugucuuu aaaagaaggucaaaucaaugauaugauuuuaucucuucuuag
SEQ ID NO.31:agguagacuuauaauuagagaaaacaacagaguuguuauuucuagugauguucuuguu aacaacuaaacgaacaauguuuguuuuucuuguuuuauugcc
SEQ ID NO.32:ugccacuagucucuagucaguguguuaaucuuacaaccagaacucaauuacccccugca uacacuaauucuuucacacgugguguuuauuacccugacaa
SEQ ID NO.33:aaguuuucagauccucaguuuuacauucaacucaggacuuguucuuaccuuucuuuuc caauguuacuugguuccaugcuauacaugucucugggaccaa
SEQ ID NO.34:augguacuaagagguuugauaacccuguccuaccauuuaaugaugguguuuauuuugc uuccacugagaagucuaacauaauaagaggcuggauuuuugg
SEQ ID NO.35:gguacuacuuuagauucgaagacccagucccuacuuauuguuaauaacgcuacuaaugu uguuauuaaagucugugaauuucaauuuuguaaugauccau
SEQ ID NO.36:uggguguuuauuaccacaaaaacaacaaaaguuggauggaaagugaguucagaguuua uucuagugcgaauaauugcacuuuugaauaugucucucagcc
SEQ ID NO.37:cagccuuuucuuauggaccuugaaggaaaacaggguaauuucaaaaaucuuagggaauu uguguuuaagaauauugaugguuauuuuaaaauauauucua
SEQ ID NO.38:agcacacgccuauuaauuuagugcgugaucucccucaggguuuuucggcuuuagaacc auugguagauuugccaauagguauuaacaucacuagguuuca
SEQ ID NO.39:aaacuuuacuugcuuuacauagaaguuauuugacuccuggugauucuucuucagguug gacagcuggugcugcagcuuauuauguggguuaucuucaacc
SEQ ID NO.40:cuaggacuuuucuauuaaaauauaaugaaaauggaaccauuacagaugcuguagacugu gcacuugacccucucucagaaacaaaguguacguugaaauc
SEQ ID NO.41:ccuucacuguagaaaaaggaaucuaucaaacuucuaacuuuagaguccaaccaacagaau cuauuguuagauuuccuaauauuacaaacuugugcccuuu
SEQ ID NO.42:ggugaaguuuuuaacgccaccagauuugcaucuguuuaugcuuggaacaggaagagaa ucagcaacuguguugcugauuauucuguccuauauaauuccg
SEQ ID NO.43:ccgcaucauuuuccacuuuuaaguguuauggagugucuccuacuaaauuaaaugaucu cugcuuuacuaaugucuaugcagauucauuuguaauuagagg
SEQ ID NO.44:ggugaugaagucagacaaaucgcuccagggcaaacuggaaagauugcugauuauaauua uaaauuaccagaugauuuuacaggcugcguuauagcuugga
SEQ ID NO.45:auucuaacaaucuugauucuaagguuggugguaauuauaauuaccuguauagauuguu uaggaagucuaaucucaaaccuuuugagagagauauuucaac
SEQ ID NO.46:cugaaaucuaucaggccgguagcacaccuuguaaugguguugaagguuuuaauuguua cuuuccuuuacaaucauaugguuuccaacccacuaauggugu
SEQ ID NO.47:guugguuaccaaccauacagaguaguaguacuuucuuuugaacuucuacaugcaccagc aacuguuuguggaccuaaaaagucuacuaauuugguuaaaa
SEQ ID NO.48:acaaaugugucaauuucaacuucaaugguuuaacaggcacagguguucuuacugaguc uaacaaaaaguuucugccuuuccaacaauuuggcagagacau
SEQ ID NO.49:uugcugacacuacugaugcuguccgugauccacagacacuugagauucuugacauuaca ccauguucuuuugguggugucaguguuauaacaccaggaac
SEQ ID NO.50:caaauacuucuaaccagguugcuguucuuuaucaggauguuaacugcacagaagucccu guugcuauucaugcagaucaacuuacuccuacuuggcgugu
SEQ ID NO.51:uuuauucuacagguucuaauguuuuucaaacacgugcaggcuguuuaauaggggcuga acaugucaacaacucauaugagugugacauacccauuggugc
SEQ ID NO.52:cagguauaugcgcuaguuaucagacucagacuaauucuccucggcgggcacguagugu agcuagucaauccaucauugccuacacuaugucacuuggugc
SEQ ID NO.53:cagaaaauucaguugcuuacucuaauaacucuauugccauacccacaaauuuuacuauu aguguuaccacagaaauucuaccagugucuaugaccaagac
SEQ ID NO.54:caucaguagauuguacaauguacauuuguggugauucaacugaaugcagcaaucuuuu guugcaauauggcaguuuuuguacacaauuaaaccgugcuuu
SEQ ID NO.55:uaacuggaauagcuguugaacaagacaaaaacacccaagaaguuuuugcacaagucaaac aaauuuacaaaacaccaccaauuaaagauuuuggugguuu
SEQ ID NO.56:uaauuuuucacaaauauuaccagauccaucaaaaccaagcaagaggucauuuauugaag aucuacuuuucaacaaagugacacuugcagaugcuggcuuc
SEQ ID NO.57:ucaucaaacaauauggugauugccuuggugauauugcugcuagagaccucauuugugc acaaaaguuuaacggccuuacuguuuugccaccuuugcucac
SEQ ID NO.58:cagaugaaaugauugcucaauacacuucugcacuguuagcggguacaaucacuucuggu uggaccuuuggugcaggugcugcauuacaaauaccauuugc
SEQ ID NO.59:cuaugcaaauggcuuauagguuuaaugguauuggaguuacacagaauguucucuauga gaaccaaaaauugauugccaaccaauuuaauagugcuauugg
SEQ ID NO.60:ggcaaaauucaagacucacuuucuuccacagcaagugcacuuggaaaacuucaagaugu ggucaaccaaaaugcacaagcuuuaaacacgcuuguuaaac
SEQ ID NO.61:aacuuagcuccaauuuuggugcaauuucaaguguuuuaaaugauauccuuucacgucu ugacaaaguugaggcugaagugcaaauugauagguugaucac
SEQ ID NO.62:caggcagacuucaaaguuugcagacauaugugacucaacaauuaauuagagcugcagaa aucagagcuucugcuaaucuugcugcuacuaaaaugucaga
SEQ ID NO.63:gaguguguacuuggacaaucaaaaagaguugauuuuuguggaaagggcuaucaucuua uguccuucccucagucagcaccucaugguguagucuucuugc
SEQ ID NO.64:augugacuuaugucccugcacaagaaaagaacuucacaacugcuccugccauuugucau gauggaaaagcacacuuuccucgugaaggugucuuuguuuc
SEQ ID NO.65:caaauggcacacacugguuuguaacacaaaggaauuuuuaugaaccacaaaucauuacu acagacaacacauuugugucugguaacugugauguuguaau
SEQ ID NO.66:uaggaauugucaacaacacaguuuaugauccuuugcaaccugaauuagacucauucaag gaggaguuagauaaauauuuuaagaaucauacaucaccaga
SEQ ID NO.67:auguugauuuaggugacaucucuggcauuaaugcuucaguuguaaacauucaaaaaga aauugaccgccucaaugagguugccaagaauuuaaaugaauc
SEQ ID NO.68:cucucaucgaucuccaagaacuuggaaaguaugagcaguauauaaaauggccaugguac auuuggcuagguuuuauagcuggcuugauugccauaguaau
SEQ ID NO.69:ggugacaauuaugcuuugcuguaugaccaguugcuguaguugucucaagggcuguug uucuuguggauccugcugcaaauuugaugaagacgacucugag
SEQ ID NO.70:gagccagugcucaaaggagucaaauuacauuacacauaaacgaacuuauggauuuguuu augagaaucuucacaauuggaacuguaacuuugaagcaagg
SEQ ID NO.71:gugaaaucaaggaugcuacuccuucagauuuuguucgcgcuacugcaacgauaccgaua caagccucacucccuuucggauggcuuauuguuggcguugc
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SEQ ID NO.73:guuguuguuuguaacaguuuacucacaccuuuugcucguugcugcuggccuugaagcc ccuuuucucuaucuuuaugcuuuagucuacuucuugcagagu
SEQ ID NO.74:guauaaacuuuguaagaauaauaaugaggcuuuggcuuugcuggaaaugccguuccaa aaacccauuacuuuaugaugccaacuauuuucuuugcuggca
SEQ ID NO.75:uacuaauuguuacgacuauuguauaccuuacaauaguguaacuucuucaauugucauu acuucaggugauggcacaacaaguccuauuucugaacaugac
SEQ ID NO.76:cuaccagauuggugguuauacugaaaaaugggaaucuggaguaaaagacuguguugua uuacacaguuacuucacuucagacuauuaccagcuguacuca
SEQ ID NO.77:acucaauugaguacagacacugguguugaacauguuaccuucuucaucuacaauaaaau uguugaugagccugaagaacauguccaaauucacacaaucg
SEQ ID NO.78:cgacgguucauccggaguuguuaauccaguaauggaaccaauuuaugaugaaccgacga cgacuacuagcgugccuuuguaagcacaagcugaugaguac
SEQ ID NO.79:cgaacuuauguacucauucguuucggaagagacagguacguuaauaguuaauagcgua cuucuuuuucuugcuuucgugguauucuugcuaguuacacua
SEQ ID NO.80:gccauccuuacugcgcuucgauugugugcguacugcugcaauauuguuaacgugaguc uuguaaaaccuucuuuuuacguuuacucucguguuaaaaauc
SEQ ID NO.81:ucugaauucuucuagaguuccugaucuucuggucuaaacgaacuaaauauuauauuag uuuuucuguuuggaacuuuaauuuuagccauggcagauucca
SEQ ID NO.82:cgguacuauuaccguugaagagcuuaaaaagcuccuugaacaauggaaccuaguaauag guuuccuauuccuuacauggauuugucuucuacaauuugcc
SEQ ID NO.83:gccuaugccaacaggaauagguuuuuguauauaauuaaguuaauuuuccucuggcugu uauggccaguaacuuuagcuuguuuugugcuugcugcuguuu
SEQ ID NO.84:cagaauaaauuggaucaccgguggaauugcuaucgcaauggcuugucuuguaggcuug auguggcucagcuacuucauugcuucuuucagacuguuugcg
SEQ ID NO.85:gcgcguacgcguuccauguggucauucaauccagaaacuaacauucuucucaacgugcc acuccauggcacuauucugaccagaccgcuucuagaaagug
SEQ ID NO.86:gaacucguaaucggagcugugauccuucguggacaucuucguauugcuggacaccauc uaggacgcugugacaucaaggaccugccuaaagaaaucacug
SEQ ID NO.87:guugcuacaucacgaacgcuuucuuauuacaaauugggagcuucgcagcguguagcag gugacucagguuuugcugcauacagucgcuacaggauuggca
SEQ ID NO.88:gcaacuauaaauuaaacacagaccauuccaguagcagugacaauauugcuuugcuugua caguaagugacaacagauguuucaucucguugacuuucagg
SEQ ID NO.89:uacuauagcagagauauuacuaauuauuaugaggacuuuuaaaguuuccauuuggaau cuugauuacaucauaaaccucauaauuaaaaauuuaucuaag
SEQ ID NO.90:aaagguuuauaccuucccagguaacaaaccaaccaacuuucgaucucuuguagaucugu ucucuaaacgaacuuuaaaaucuguguggcugucacucggc
SEQ ID NO.91:cggcugcaugcuuagugcacucacgcaguauaauuaauaacuaauuacugucguugaca ggacacgaguaacucgucuaucuucugcaggcugcuuacgg
SEQ ID NO.92:uuucguccguguugcagccgaucaucagcacaucuagguuucguccgggugugaccga aagguaagauggagagccuugucccugguuucaacgagaaaa
SEQ ID NO.93:aacacacguccaacucaguuugccuguuuuacagguucgcgacgugcucguacguggc uuuggagacuccguggaggaggucuuaucagaggcacgucaa
SEQ ID NO.94:acaucuuaaagauggcacuuguggcuuaguagaaguugaaaaaggcguuuugccucaa cuugaacagcccuauguguucaucaaacguucggaugcucga
SEQ ID NO.95:aacugcaccucauggucauguuaugguugagcugguagcagaacucgaaggcauucag uacggucguaguggugagacacuugguguccuugucccucau
SEQ ID NO.96:gugggcgaaauaccaguggcuuaccgcaagguucuucuucguaagaacgguaauaaag gagcugguggccauaguuacggcgccgaucuaaagucauuug
SEQ ID NO.97:gacuuaggcgacgagcuuggcacugauccuuaugaagauuuucaagaaaacuggaacac uaaacauagcagugguguuacccgugaacucaugcgugagc
SEQ ID NO.98:cuuaacggaggggcauacacucgcuaugucgauaacaacuucuguggcccugauggcua cccucuugagugcauuaaagaccuucuagcacgugcuggua
SEQ ID NO.99:aaagcuucaugcacuuuguccgaacaacuggacuuuauugacacuaagagggguguaua cugcugccgugaacaugagcaugaaauugcuugguacacgg
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SEQ ID NO.101:cccuuaaauuccauaaucaagacuauucaaccaaggguugaaaagaaaaagcuugaug gcuuuauggguagaauucgaucugucuauccaguugcgucac
SEQ ID NO.102:ccaaaugaaugcaaccaaaugugccuuucaacucucaugaagugugaucauuguggug aaacuucauggcagacgggcgauuuuguuaaagccacuugcg
SEQ ID NO.103:cgaauuuuguggcacugagaauuugacuaaagaaggugccacuacuugugguuacuu accccaaaaugcuguuguuaaaauuuauuguccagcaugucac
SEQ ID NO.104:caauucagaaguaggaccugagcauagucuugccgaauaccauaaugaaucuggcuug aaaaccauucuucguaaggguggucgcacuauugccuuugga
SEQ ID NO.105:ggcuguguguucucuuauguugguugccauaacaagugugccuauuggguuccacg ugcuagcgcuaacauagguuguaaccauacagguguuguuggag
SEQ ID NO.106:gaagguuccgaaggucuuaaugacaaccuucuugaaauacuccaaaaagagaaaguca acaucaauauuguuggugacuuuaaacuuaaugaagagaucg
SEQ ID NO.107:cgccauuauuuuggcaucuuuuucugcuuccacaagugcuuuuguggaaacugugaa agguuuggauuauaaagcauucaaacaaauuguugaauccugu
SEQ ID NO.108:gguaauuuuaaaguuacaaaaggaaaagcuaaaaaaggugccuggaauauuggugaac agaaaucaauacugaguccucuuuaugcauuugcaucagagg
SEQ ID NO.109:ggcugcucguguuguacgaucaauuuucucccgcacucuugaaacugcucaaaauuc ugugcguguuuuacagaaggccgcuauaacaauacuagaugga
SEQ ID NO.110:ggaauuucacaguauucacugagacucauugaugcuaugauguucacaucugauuug gcuacuaacaaucuaguuguaauggccuacauuacagguggug
SEQ ID NO.111:guucaguugacuucgcaguggcuaacuaacaucuuuggcacuguuuaugaaaaacuc aaacccguccuugauuggcuugaagagaaguuuaaggaaggug
SEQ ID NO.112:gguguagaguuucuuagagacgguugggaaauuguuaaauuuaucucaaccugugcu ugugaaauugucgguggacaaauugucaccugugcaaaggaaa
SEQ ID NO.113:uaaggagaguguucagacauucuuuaagcuuguaaauaaauuuuuggcuuugugugc ugacucuaucauuauugguggagcuaaacuuaaagccuugaau
SEQ ID NO.114:uuaggugaaacauuugucacgcacucaaagggauuguacagaaaguguguuaaaucca gagaagaaacuggccuacucaugccucuaaaagccccaaaag
SEQ ID NO.115:agaaauuaucuucuuagagggagaaacacuucccacagaaguguuaacagaggaaguu gucuugaaaacuggugauuuacaaccauuagaacaaccuacu
SEQ ID NO.116:gugaagcuguugaagcuccauugguugguacaccaguuuguauuaacgggcuuaugu ugcucgaaaucaaagacacagaaaaguacugugcccuugcacc
SEQ ID NO.117:accuaauaugaugguaacaaacaauaccuucacacucaaaggcggugcaccaacaaagg uuacuuuuggugaugacacugugauagaagugcaagguuac
SEQ ID NO.118:caagagugugaauaucacuuuugaacuugaugaaaggauugauaaaguacuuaauga gaagugcucugccuauacaguugaacucgguacagaaguaaau
SEQ ID NO.119:gaguucgccuguguuguggcagaugcugucauaaaaacuuugcaaccaguaucugaa uuacuuacaccacugggcauugauuuagaugaguggaguaugg
SEQ ID NO.120:ggcuacauacuacuuauuugaugagucuggugaguuuaaauuggcuucacauaugua uuguucuuucuacccuccagaugaggaugaagaagaaggugau
SEQ ID NO.121:ugugaagaagaagaguuugagccaucaacucaauaugaguaugguacugaagaugau uaccaagguaaaccuuuggaauuuggugccacuucugcugcuc
SEQ ID NO.122:cucuucaaccugaagaagagcaagaagaagauugguuagaugaugauagucaacaaac uguuggucaacaagacggcagugaggacaaucagacaacuac
SEQ ID NO.123:cuauucaaacaauuguugagguucaaccucaauuagagauggaacuuacaccaguugu ucagacuauugaagugaauaguuuuagugguuauuuaaaacu
SEQ ID NO.124:cugacaauguauacauuaaaaaugcagacauuguggaagaagcuaaaaagguaaaacc aacagugguuguuaaugcagccaauguuuaccuuaaacaugg
SEQ ID NO.125:ggaggagguguugcaggagccuuaaauaaggcuacuaacaaugccaugcaaguugaa ucugaugauuacauagcuacuaauggaccacuuaaagugggug
SEQ ID NO.126:gugguaguuguguuuuaagcggacacaaucuugcuaaacacugucuucauguugucg gcccaaauguuaacaaaggugaagacauucaacuucuuaagag
SEQ ID NO.127:gugcuuaugaaaauuuuaaucagcacgaaguucuacuugcaccauuauuaucagcug guauuuuuggugcugacccuauacauucuuuaagaguuugugu
SEQ ID NO.128:gauacuguucgcacaaaugucuacuuagcugucuuugauaaaaaucucuaugacaaac uuguuucaagcuuuuuggaaaugaagagugaaaagcaaguug
SEQ ID NO.129:ugaacaaaagaucgcugagauuccuaaagaggaaguuaagccauuuauaacugaaagu aaaccuucaguugaacagagaaaacaagaugauaagaaaauc
SEQ ID NO.130:caaagcuuguguugaagaaguuacaacaacucuggaagaaacuaaguuccucacagaa aacuuguuacuuuauauugacauuaauggcaaucuucaucca
SEQ ID NO.131:cagauucugccacucuuguuagugacauugacaucacuuucuuaaagaaagaugcucc auauauagugggugauguuguucaagaggguguuuuaacugc
SEQ ID NO.132:cugugguuauaccuacuaaaaaggcugguggcacuacugaaaugcuagcgaaagcuu ugagaaaagugccaacagacaauuauauaaccacuuacccggg
SEQ ID NO.133:caggguuuaaaugguuacacuguagaggaggcaaagacagugcuuaaaaaguguaaaa gugccuuuuacauucuaccaucuauuaucucuaaugagaagc
SEQ ID NO.134:gcaagaaauucuuggaacuguuucuuggaauuugcgagaaaugcuugcacaugcaga agaaacacgcaaauuaaugccugucuguguggaaacuaaagcc
SEQ ID NO.135:ccauaguuucaacuauacagcguaaauauaaggguauuaaaauacaagaggguguggu ugauuauggugcuagauuuuacuuuuacaccaguaaaacaac
SEQ ID NO.136:cuguagcgucacuuaucaacacacuuaacgaucuaaaugaaacucuuguuacaaugcc acuuggcuauguaacacauggcuuaaauuuggaagaagcugc
SEQ ID NO.137:cucgguauaugagaucucucaaagugccagcuacaguuucuguuucuucaccugaug cuguuacagcguauaaugguuaucuuacuucuucuucuaaaac
SEQ ID NO.138:accugaagaacauuuuauugaaaccaucucacuugcugguuccuauaaagauuggucc uauucuggacaaucuacacaacuagguauagaauuucuuaag
SEQ ID NO.139:gagaggugauaaaaguguauauuacacuaguaauccuaccacauuccaccuagauggu gaaguuaucaccuuugacaaucuuaagacacuucuuucuuug
SEQ ID NO.140:gagagaagugaggacuauuaagguguuuacaacaguagacaacauuaaccuccacacg caaguuguggacaugucaaugacauauggacaacaguuuggu
SEQ ID NO.141:guccaacuuauuuggauggagcugauguuacuaaaauaaaaccucauaauucacauga agguaaaacauuuuauguuuuaccuaaugaugacacucuacg
SEQ ID NO.142:cguguugaggcuuuugaguacuaccacacaacugauccuaguuuucuggguagguac augucagcauuaaaucacacuaaaaaguggaaauacccacaag
SEQ ID NO.143:aaugguuuaacuucuauuaaaugggcagauaacaacuguuaucuugccacugcauug uuaacacuccaacaaauagaguugaaguuuaauccaccugcuc
SEQ ID NO.144:gcucuacaagaugcuuauuacagagcaagggcuggugaagcugcuaacuuuugugca cuuaucuuagccuacuguaauaagacaguaggugaguuaggug
SEQ ID NO.145:uguuagagaaacaaugaguuacuuguuucaacaugccaauuuagauucuugcaaaaga gucuugaacgugguguguaaaacuuguggacaacagcagaca
SEQ ID NO.146:caacccuuaaggguguagaagcuguuauguacaugggcacacuuucuuaugaacaau uuaagaaagguguucagauaccuuguacgugugguaaacaagc
SEQ ID NO.147:uacaaaauaucuaguacaacaggagucaccuuuuguuaugaugucagcaccaccugcu caguaugaacuuaagcaugguacauuuacuugugcuagugag
SEQ ID NO.148:guacacugguaauuaccaguguggucacuauaaacauauaacuucuaaagaaacuuug uauugcauagacggugcuuuacuuacaaaguccucagaauac
SEQ ID NO.149:caaagguccuauuacggauguuuucuacaaagaaaacaguuacacaacaaccauaaaac caguuacuuauaaauuggaugguguuguuuguacagaaauu
SEQ ID NO.150:gacccuaaguuggacaauuauuauaagaaagacaauucuuauuucacagagcaaccaa uugaucuuguaccaaaccaaccauauccaaacgcaagcuucg
SEQ ID NO.151:cgauaauuuuaaguuuguaugugauaauaucaaauuugcugaugauuuaaaccaguu aacugguuauaagaaaccugcuucaagagagcuuaaaguuaca
SEQ ID NO.152:cauuuuucccugacuuaaauggugaugugguggcuauugauuauaaacacuacacac ccucuuuuaagaaaggagcuaaauuguuacauaaaccuauugu
SEQ ID NO.153:uuggcauguuaacaaugcaacuaauaaagccacguauaaaccaaauaccugguguaua cguugucuuuggagcacaaaaccaguugaaacaucaaauucg
SEQ ID NO.154:cguuugauguacugaagucagaggacgcgcagggaauggauaaucuugccugcgaag aucuaaaaccagucucugaagaaguaguggaaaauccuaccau
SEQ ID NO.155:cagaaagacguucuugaguguaaugugaaaacuaccgaaguuguaggagacauuauac uuaaaccagcaaauaauaguuuaaaaauuacagaagagguug
SEQ ID NO.156:uggccacacagaucuaauggcugcuuauguagacaauucuagucuuacuauuaagaaa ccuaaugaauuaucuagaguauuagguuugaaaacccuugcu
SEQ ID NO.157:gcuacucaugguuuagcugcuguuaauagugucccuugggauacuauagcuaauuau gcuaagccuuuucuuaacaaaguuguuaguacaacuacuaaca
SEQ ID NO.158:aguuacacgguguuuaaaccguguuuguacuaauuauaugccuuauuucuuuacuuu auugcuacaauuguguacuuuuacuagaaguacaaauucuaga
SEQ ID NO.159:gaauuaaagcaucuaugccgacuacuauagcaaagaauacuguuaagagugucgguaa auuuugucuagaggcuucauuuaauuauuugaagucaccuaa
SEQ ID NO.160:uuuuucuaaacugauaaauauuauaauuugguuuuuacuauuaaguguuugccuagg uucuuuaaccuacucaaccgcugcuuuagguguuuuaaugucu
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SEQ ID NO.162:guguuugucuuagugguuuagauucuuuagacaccuauccuucuuuagaaacuauac aaauuaccauuucaucuuuuaaaugggauuuaacugcuuuugg
SEQ ID NO.163:ggcuuaguugcagagugguuuuuggcauauauucuuuucacuagguuuuucuaugu acuuggauuggcugcaaucaugcaauuguuuuucagcuauuuug
SEQ ID NO.164:gcaguacauuuuauuaguaauucuuggcuuaugugguuaauaauuaaucuuguacaa auggccccgauuucagcuaugguuagaauguacaucuucuuug
SEQ ID NO.165:gcaucauuuuauuauguauggaaaaguuaugugcauguuguagacgguuguaauuca ucaacuuguaugauguguuacaaacguaauagagcaacaagag
SEQ ID NO.166:gagucgaauguacaacuauuguuaaugguguuagaagguccuuuuaugucuaugcua auggagguaaaggcuuuugcaaacuacacaauuggaauugugu
SEQ ID NO.167:uaauugugauacauucugugcugguaguacauuuauuagugaugaaguugcgagaga cuugucacuacaguuuaaaagaccaauaaauccuacugaccag
SEQ ID NO.168:gucuucuuacaucguugauaguguuacagugaagaaugguuccauccaucuuuacuu ugauaaagcuggucaaaagacuuaugaaagacauucucucucu
SEQ ID NO.169:cucauuuuguuaacuuagacaaccugagagcuaauaacacuaaagguucauugccuau uaauguuauaguuuuugaugguaaaucaaaaugugaagaauc
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SEQ ID NO.171:guugcaguuaaaauguuugaugcuuacguuaauacguuuucaucaacuuuuaacgua ccaauggaaaaacucaaaacacuaguugcaacugcagaagcug
SEQ ID NO.172:gcugaacuugcaaagaauguguccuuagacaaugucuuaucuacuuuuauuucagca gcucggcaaggguuuguugauucagauguagaaacuaaagaug
SEQ ID NO.173:guugaaugucuuaaauugucacaucaaucugacauagaaguuacuggcgauaguugu aauaacuauaugcucaccuauaacaaaguugaaaacaugacac
SEQ ID NO.174:caccccgugaccuuggugcuuguauugacuguagugcgcgucauauuaaugcgcagg uagcaaaaagucacaacauugcuuugauauggaacguuaaaga
SEQ ID NO.175:uucaugucauugucugaacaacuacgaaaacaaauacguagugcugcuaaaaagaaua acuuaccuuuuaaguugacaugugcaacuacuagacaaguug
SEQ ID NO.176:guuaauguuguaacaacaaagauagcacuuaagggugguaaaauuguuaauaauugg uugaagcaguuaauuaaaguuacacuuguguuccuuuuuguug
SEQ ID NO.177:ugcugcuauuuucuauuuaauaacaccuguucaugucaugucuaaacauacugacuu uucaagugaaaucauaggauacaaggcuauugauggugguguc
SEQ ID NO.178:cacucgugacauagcaucuacagauacuuguuuugcuaacaaacaugcugauuuugac acaugguuuagccagcguggugguaguuauacuaaugacaaa
SEQ ID NO.179:gcuugcccauugauugcugcagucauaacaagagaaguggguuuugucgugccuggu uugccuggcacgauauuacgcacaacuaauggugacuuuuugc
SEQ ID NO.180:gcauuucuuaccuagaguuuuuagugcaguugguaacaucuguuacacaccaucaaaa cuuauagaguacacugacuuugcaacaucagcuuguguuuug
SEQ ID NO.181:ggcugcugaauguacaauuuuuaaagaugcuucugguaagccaguaccauauuguua ugauaccaauguacuagaagguucuguugcuuaugaaaguuua
SEQ ID NO.182:cgcccugacacacguuaugugcucauggauggcucuauuauucaauuuccuaacaccu accuugaagguucuguuagagugguaacaacuuuugauucug
SEQ ID NO.183:cugaguacuguaggcacggcacuugugaaagaucagaagcugguguuuguguaucua cuagugguagauggguacuuaacaaugauuauuacagaucuuu
SEQ ID NO.184:ccaggaguuuucugugguguagaugcuguaaauuuacuuacuaauauguuuacacca cuaauucaaccuauuggugcuuuggacauaucagcaucuauag
SEQ ID NO.185:aguagcuggugguauuguagcuaucguaguaacaugccuugccuacuauuuuaugag guuuagaagagcuuuuggugaauacagucauguaguugccuuu
SEQ ID NO.186:uaauacuuuacuauuccuuaugucauucacuguacucuguuuaacaccaguuuacuca uucuuaccugguguuuauucuguuauuuacuuguacuugaca
SEQ ID NO.187:cauuuuaucuuacuaaugauguuucuuuuuuagcacauauucaguggaugguuaugu ucacaccuuuaguaccuuucuggauaacaauugcuuauaucau
SEQ ID NO.188:uguauuuccacaaagcauuucuauugguucuuuaguaauuaccuaaagagacgugua gucuuuaaugguguuuccuuuaguacuuuugaagaagcugcgc
SEQ ID NO.189:gcgcugugcaccuuuuuguuaaauaaagaaauguaucuaaaguugcguagugaugug cuauuaccucuuacgcaauauaauagauacuuagcucuuuaua
SEQ ID NO.190:uaaguacaaguauuuuaguggagcaauggauacaacuagcuacagagaagcugcuug uugucaucucgcaaaggcucucaaugacuucaguaacucaggu
SEQ ID NO.191:guucugauguucuuuaccaaccaccacaaaccucuaucaccucagcuguuuugcagag ugguuuuagaaaaauggcauucccaucugguaaaguugaggg
SEQ ID NO.192:uuguaugguacaaguaacuugugguacaacuacacuuaacggucuuuggcuugauga cguaguuuacuguccaagacaugugaucugcaccucugaagac
SEQ ID NO.193:caugcuuaacccuaauuaugaagauuuacucauucguaagucuaaucauaauuucuug guacaggcugguaauguucaacucaggguuauuggacauucu
SEQ ID NO.194:uaugcaaaauuguguacuuaagcuuaagguugauacagccaauccuaagacaccuaag uauaaguuuguucgcauucaaccaggacagacuuuuucagug
SEQ ID NO.195:guuagcuuguuacaaugguucaccaucugguguuuaccaaugugcuaugaggcccaa uuucacuauuaaggguucauuccuuaaugguucaugugguagu
SEQ ID NO.196:uguugguuuuaacauagauuaugacugugucucuuuuuguuacaugcaccauaugga auuaccaacuggaguucaugcuggcacagacuuagaagguaac
SEQ ID NO.197:cuuuuauggaccuuuuguugacaggcaaacagcacaagcagcugguacggacacaacu auuacaguuaauguuuuagcuugguuguacgcugcuguuaua
SEQ ID NO.198:aauggagacaggugguuucucaaucgauuuaccacaacucuuaaugacuuuaaccuug uggcuaugaaguacaauuaugaaccucuaacacaagaccaug
SEQ ID NO.199:uguugacauacuaggaccucuuucugcucaaacuggaauugccguuuuagauaugug ugcuucauuaaaagaauuacugcaaaaugguaugaauggacgu
SEQ ID NO.200:guaccauauuggguagugcuuuauuagaagaugaauuuacaccuuuugauguuguua gacaaugcucagguguuacuuuccaaagugcagugaaaagaac
SEQ ID NO.201:caaucaaggguacacaccacugguuguuacucacaauuuugacuucacuuuuaguuu uaguccagaguacucaauggucuuuguucuuuuuuuuguauga
SEQ ID NO.202:augccuuuuuaccuuuugcuauggguauuauugcuaugucugcuuuugcaaugaug uuugucaaacauaagcaugcauuucucuguuuguuuuuguuacc
SEQ ID NO.203:accuucucuugccacuguagcuuauuuuaauauggucuauaugccugcuaguugggu gaugcguauuaugacaugguuggauaugguugauacuaguuug
SEQ ID NO.204:gucugguuuuaagcuaaaagacuguguuauguaugcaucagcuguaguguuacuaau ccuuaugacagcaagaacuguguaugaugauggugcuaggaga
SEQ ID NO.205:gaguguggacacuuaugaaugucuugacacucguuuauaaaguuuauuaugguaaug cuuuagaucaagccauuuccaugugggcucuuauaaucucugu
SEQ ID NO.206:uacuucuaacuacucagguguaguuacaacugucauguuuuuggccagagguauugu uuuuauguguguugaguauugcccuauuuucuucauaacuggu
SEQ ID NO.207:uaauacacuucaguguauaaugcuaguuuauuguuucuuaggcuauuuuuguacuug uuacuuuggccucuuuuguuuacucaaccgcuacuuuagacug
SEQ ID NO.208:gacucuugguguuuaugauuacuuaguuucuacacaggaguuuagauauaugaauuc acagggacuacucccacccaagaauagcauagaugccuucaaa
SEQ ID NO.209:cucaacauuaaauuguuggguguugguggcaaaccuuguaucaaaguagccacugua cagucuaaaaugucagauguaaagugcacaucaguagucuuac
SEQ ID NO.210:cucucaguuuugcaacaacucagaguagaaucaucaucuaaauugugggcucaaugug uccaguuacacaaugacauucucuuagcuaaagauacuacug
SEQ ID NO.211:gaagccuuugaaaaaaugguuucacuacuuucuguuuugcuuuccaugcagggugcu guagacauaaacaagcuuugugaagaaaugcuggacaacaggg
SEQ ID NO.212:gggcaaccuuacaagcuauagccucagaguuuaguucccuuccaucauaugcagcuuu ugcuacugcucaagaagcuuaugagcaggcuguugcuaaugg
SEQ ID NO.213:gauucugaaguuguucuuaaaaaguugaagaagucuuugaauguggcuaaaucugaa uuugaccgugaugcagccaugcaacguaaguuggaaaagaugg
SEQ ID NO.214:ggcugaucaagcuaugacccaaauguauaaacaggcuagaucugaggacaagagggca aaaguuacuagugcuaugcagacaaugcuuuucacuaugcuu
SEQ ID NO.215:uagaaaguuggauaaugaugcacucaacaacauuaucaacaaugcaagagaugguugu guucccuugaacauaauaccucuuacaacagcagccaaacua
SEQ ID NO.216:ugguugucauaccagacuauaacacauauaaaaauacgugugaugguacaacauuuac uuaugcaucagcauugugggaaauccaacagguuguagaugc
SEQ ID NO.217:gcagauaguaaaauuguucaacuuagugaaauuaguauggacaauucaccuaauuuag cauggccucuuauuguaacagcuuuaagggccaauucugcug
SEQ ID NO.218:gcugucaaauuacagaauaaugagcuuaguccuguugcacuacgacagaugucuugu gcugccgguacuacacaaacugcuugcacugaugacaaugcgu
SEQ ID NO.219:gcuuacuacaacacaacaaagggagguagguuuguacuugcacuguuauccgauuuac aggauuugaaaugggcuagauucccuaagagugauggaacug
SEQ ID NO.220:ugguacuaucuauacagaacuggaaccaccuuguagguuuguuacagacacaccuaaa gguccuaaagugaaguauuuauacuuuauuaaaggauuaaac
SEQ ID NO.221:caaccuaaauagagguaugguacuugguaguuuagcugccacaguacgucuacaagcu gguaaugcaacagaagugccugccaauucaacuguauuaucu
SEQ ID NO.222:uuucugugcuuuugcuguagaugcugcuaaagcuuacaaagauuaucuagcuagugg gggacaaccaaucacuaauuguguuaagauguuguguacacac
SEQ ID NO.223:cacugguacuggucaggcaauaacaguuacaccggaagccaauauggaucaagaaucc uuugguggugcaucguguugucuguacugccguugccacaua
SEQ ID NO.224:agaucauccaaauccuaaaggauuuugugacuuaaaagguaaguauguacaaauaccu acaacuugugcuaaugacccuguggguuuuacacuuaaaaac
SEQ ID NO.225:cacagucuguaccgucugcgguauguggaaagguuauggcuguaguugugaucaacu ccgcgaacccaugcuucagucagcugaugcacaaucguuuuua
SEQ ID NO.226:aaacggguuugcgguguaagugcagcccgucuuacaccgugcggcacaggcacuagu acugaugucguauacagggcuuuugacaucuacaaugauaaag
SEQ ID NO.227:guagcugguuuugcuaaauuccuaaaaacuaauuguugucgcuuccaagaaaaggac gaagaugacaauuuaauugauucuuacuuuguaguuaagagac
SEQ ID NO.228:cacacuuucucuaacuaccaacaugaagaaacaauuuauaauuuacuuaaggauuguc cagcuguugcuaaacaugacuucuuuaaguuuagaauagacg
SEQ ID NO.229:cggugacaugguaccacauauaucacgucaacgucuuacuaaauacacaauggcagac cucgucuaugcuuuaaggcauuuugaugaagguaauugugac
SEQ ID NO.230:acauuaaaagaaauacuugucacauacaauuguugugaugaugauuauuucaauaaaa aggacugguaugauuuuguagaaaacccagauauauuacgcg
SEQ ID NO.231:gcguauacgccaacuuaggugaacguguacgccaagcuuuguuaaaaacaguacaauu cugugaugccaugcgaaaugcugguauuguugguguacugac
SEQ ID NO.232:uuagauaaucaagaucucaaugguaacugguaugauuucggugauuucauacaaacca cgccagguaguggaguuccuguuguagauucuuauuauucau
SEQ ID NO.233:uuguuaaugccuauauuaaccuugaccagggcuuuaacugcagagucacauguugac acugacuuaacaaagccuuacauuaagugggauuuguuaaaau
SEQ ID NO.234:ugacuucacggaagagagguuaaaacucuuugaccguuauuuuaaauauugggauca gacauaccacccaaauuguguuaacuguuuggaugacagaugc
SEQ ID NO.235:gcauucugcauugugcaaacuuuaauguuuuauucucuacaguguucccaccuacaa guuuuggaccacuagugagaaaaauauuuguugaugguguucc
SEQ ID NO.236:ccauuuguaguuucaacuggauaccacuucagagagcuagguguuguacauaaucag gauguaaacuuacauagcucuagacuuaguuuuaaggaauuac
SEQ ID NO.237:cuuguguaugcugcugacccugcuaugcacgcugcuucugguaaucuauuacuagau aaacgcacuacgugcuuuucaguagcugcacuuacuaacaaug
SEQ ID NO.238:guugcuuuucaaacugucaaacccgguaauuuuaacaaagacuucuaugacuuugcu gugucuaaggguuucuuuaaggaaggaaguucuguugaauuaa
SEQ ID NO.239:aaacacuucuucuuugcucaggaugguaaugcugcuaucagcgauuaugacuacuau cguuauaaucuaccaacaaugugugauaucagacaacuacuau
SEQ ID NO.240:uuuguaguugaaguuguugauaaguacuuugauuguuacgaugguggcuguauuaa ugcuaaccaagucaucgucaacaaccuagacaaaucagcugguu
SEQ ID NO.241:uuccauuuaauaaaugggguaaggcuagacuuuauuaugauucaaugaguuaugagg aucaagaugcacuuuucgcauauacaaaacguaaugucauccc
SEQ ID NO.242:ccuacuauaacucaaaugaaucuuaaguaugccauuagugcaaagaauagagcucgca ccguagcuggugucucuaucuguaguacuaugaccaauagac
SEQ ID NO.243:caguuucaucaaaaauuauugaaaucaauagccgccacuagaggagcuacuguaguaa uuggaacaagcaaauucuauggugguuggcacaacauguuaa
SEQ ID NO.244:aaaacuguuuauagugauguagaaaacccucaccuuauggguugggauuauccuaaa ugugauagagccaugccuaacaugcuuagaauuauggccucac
SEQ ID NO.245:cuuguucuugcucgcaaacauacaacguguuguagcuugucacaccguuucuauaga uuagcuaaugagugugcucaaguauugagugaaauggucaugu
SEQ ID NO.246:guggcgguucacuauauguuaaaccagguggaaccucaucaggagaugccacaacugc uuaugcuaauaguguuuuuaacauuugucaagcugucacggc
SEQ ID NO.247:cggccaauguuaaugcacuuuuaucuacugaugguaacaaaauugccgauaaguaug uccgcaauuuacaacacagacuuuaugagugucucuauagaaa
SEQ ID NO.248:gagauguugacacagacuuugugaaugaguuuuacgcauauuugcguaaacauuucu caaugaugauacucucugacgaugcuguuguguguuucaauag
SEQ ID NO.249:gcacuuaugcaucucaaggucuaguggcuagcauaaagaacuuuaagucaguucuuu auuaucaaaacaauguuuuuaugucugaagcaaaauguuggac
SEQ ID NO.250:cugagacugaccuuacuaaaggaccucaugaauuuugcucucaacauacaaugcuagu uaaacagggugaugauuauguguaccuuccuuacccagaucc
SEQ ID NO.251:ccaucaagaauccuaggggccggcuguuuuguagaugauaucguaaaaacagauggu acacuuaugauugaacgguucgugucuuuagcuauagaugcuu
SEQ ID NO.252:uacccacuuacuaaacauccuaaucaggaguaugcugaugucuuucauuuguacuuac aauacauaagaaagcuacaugaugaguuaacaggacacaugu
SEQ ID NO.253:uagacauguauucuguuaugcuuacuaaugauaacacuucaagguauugggaaccug aguuuuaugaggcuauguacacaccgcauacagucuuacaggc
SEQ ID NO.254:gcuguuggggcuuguguucuuugcaauucacagacuucauuaagauguggugcuug cauacguagaccauucuuauguuguaaaugcuguuacgaccaug
SEQ ID NO.255:gucauaucaacaucacauaaauuagucuugucuguuaauccguauguuugcaaugcu ccagguugugaugucacagaugugacucaacuuuacuuaggag
SEQ ID NO.256:gguaugagcuauuauuguaaaucacauaaaccacccauuaguuuuccauugugugcu aauggacaaguuuuugguuuauauaaaaauacauguguuggua
SEQ ID NO.257:agcgauaauguuacugacuuuaaugcaauugcaacaugugacuggacaaaugcuggu gauuacauuuuagcuaacaccuguacugaaagacucaagcuuu
SEQ ID NO.258:uugcagcagaaacgcucaaagcuacugaggagacauuuaaacugucuuaugguauugc uacuguacgugaagugcugucugacagagaauuacaucuuuc
SEQ ID NO.259:caugggaaguugguaaaccuagaccaccacuuaaccgaaauuaugucuuuacugguua ucguguaacuaaaaacaguaaaguacaaauaggagaguacac
SEQ ID NO.260:accuuugaaaaaggugacuauggugaugcuguuguuuaccgagguacaacaacuuac aaauuaaauguuggugauuauuuugugcugacaucacauacag
SEQ ID NO.261:guaaugccauuaagugcaccuacacuagugccacaagagcacuauguuagaauuacug gcuuauacccaacacucaauaucucagaugaguuuucuagca
SEQ ID NO.262:caauguugcaaauuaucaaaagguugguaugcaaaaguauucuacacuccagggacca ccugguacugguaagagucauuuugcuauuggccuagcucuc
SEQ ID NO.263:cuacuacccuucugcucgcauaguguauacagcuugcucucaugccgcuguugaugc acuaugugagaaggcauuaaaauauuugccuauagauaaaugu
SEQ ID NO.264:guagaauuauaccugcacgugcucguguagaguguuuugauaaauucaaagugaauu caacauuagaacaguaugucuuuuguacuguaaaugcauugcc
SEQ ID NO.265:ccugagacgacagcagauauaguugucuuugaugaaauuucaauggccacaaauuaug auuugaguguugucaaugccagauuacgugcuaagcacuaug
SEQ ID NO.266:guguacauuggcgacccugcucaauuaccugcaccacgcacauugcuaacuaagggca cacuagaaccagaauauuucaauucaguguguagacuuauga
SEQ ID NO.267:aaacuauagguccagacauguuccucggaacuugucggcguuguccugcugaaauug uugacacugugagugcuuugguuuaugauaauaagcuuaaagc
SEQ ID NO.268:gcacauaaagacaaaucagcucaaugcuuuaaaauguuuuauaaggguguuaucacgc augauguuucaucugcaauuaacaggccacaaauaggcgugg
SEQ ID NO.269:guaagagaauuccuuacacguaacccugcuuggagaaaagcugucuuuauuucaccuu auaauucacagaaugcuguagccucaaagauuuugggacuac
SEQ ID NO.270:ccaacucaaacuguugauucaucacagggcucagaauaugacuaugucauauucacuc aaaccacugaaacagcucacucuuguaauguaaacagauuua
SEQ ID NO.271:uguugcuauuaccagagcaaaaguaggcauacuuugcauaaugucugauagagaccu uuaugacaaguugcaauuuacaagucuugaaauuccacguagg
SEQ ID NO.272:aggaauguggcaacuuuacaagcugaaaauguaacaggacucuuuaaagauuguagua agguaaucacuggguuacauccuacacaggcaccuacacacc
SEQ ID NO.273:ccucaguguugacacuaaauucaaaacugaagguuuauguguugacauaccuggcaua ccuaaggacaugaccuauagaagacucaucucuaugaugggu
SEQ ID NO.274:uaaaaugaauuaucaaguuaaugguuacccuaacauguuuaucacccgcgaagaagcu auaagacauguacgugcauggauuggcuucgaugucgagggg
SEQ ID NO.275:gggugucaugcuacuagagaagcuguugguaccaauuuaccuuuacagcuagguuuu ucuacagguguuaaccuaguugcuguaccuacagguuauguug
SEQ ID NO.276:gauacaccuaauaauacagauuuuuccagaguuagugcuaaaccaccgccuggagauc aauuuaaacaccucauaccacuuauguacaaaggacuuccuu
SEQ ID NO.277:uggaauguagugcguauaaagauuguacaaauguuaagugacacacuuaaaaaucucu cugacagagucguauuugucuuaugggcacauggcuuugagu
SEQ ID NO.278:gacaucuaugaaguauuuugugaaaauaggaccugagcgcaccuguugucuauguga uagacgugccacaugcuuuuccacugcuucagacacuuaugcc
SEQ ID NO.279:gccuguuggcaucauucuauuggauuugauuacgucuauaauccguuuaugauugau guucaacaaugggguuuuacagguaaccuacaaagcaaccaug
SEQ ID NO.280:gaucuguauugucaaguccaugguaaugcacauguagcuaguugugaugcaaucaug acuaggugucuagcuguccacgagugcuuuguuaagcguguug
SEQ ID NO.281:gacuggacuauugaauauccuauaauuggugaugaacugaagauuaaugcggcuugu agaaagguucaacacaugguuguuaaagcugcauuauuagcag
SEQ ID NO.282:gacaaauucccaguucuucacgacauugguaacccuaaagcuauuaaguguguaccuc aagcugauguagaauggaaguucuaugaugcacagccuugua
SEQ ID NO.283:agugacaaagcuuauaaaauagaagaauuauucuauucuuaugccacacauucugaca aauucacagaugguguaugccuauuuuggaauugcaaugucg
SEQ ID NO.284:cgauagauauccugcuaauuccauuguuuguagauuugacacuagagugcuaucuaa ccuuaacuugccugguugugaugguggcaguuuguauguaaau
SEQ ID NO.285:aacaugcauuccacacaccagcuuuugauaaaagugcuuuuguuaauuuaaaacaauu accauuuuucuauuacucugacaguccaugugagucucaugg
SEQ ID NO.286:ggaaaacaaguagugucagauauagauuauguaccacuaaagucugcuacguguauaa cacguugcaauuuagguggugcugucuguagacaucaugcua
SEQ ID NO.287:augaguacagauuguaucucgaugcuuauaacaugaugaucucagcuggcuuuagcu uguggguuuacaaacaauuugauacuuauaaccucuggaacac
SEQ ID NO.288:cacuuuuacaagacuucagaguuuagaaaauguggcuuuuaauguuguaaauaaggg acacuuugauggacaacagggugaaguaccaguuucuaucauu
SEQ ID NO.289:uaacacuguuuacacaaaaguugaugguguugauguagaauuguuugaaaauaaaac aacauuaccuguuaauguagcauuugagcuuugggcuaagcgc
SEQ ID NO.290:cgcaacauuaaaccaguaccagaggugaaaauacucaauaauuuggguguggacauug cugcuaauacugugaucugggacuacaaaagagaugcuccag
SEQ ID NO.291:gcacauauaucuacuauugguguuuguucuaugacugacauagccaagaaaccaacug aaacgauuugugcaccacucacugucuuuuuugaugguagag
SEQ ID NO.292:guugauggucaaguagacuuauuuagaaaugcccguaaugguguucuuauuacagaa gguaguguuaaagguuuacaaccaucuguaggucccaaacaag
SEQ ID NO.293:gcuagucuuaauggagucacauuaauuggagaagccguaaaaacacaguucaauuauu auaagaaaguugaugguguuguccaacaauuaccugaaacuu
SEQ ID NO.294:uacuuuacucagaguagaaauuuacaagaauuuaaacccaggagucaaauggaaauug auuucuuagaauuagcuauggaugaauucauugaacgguaua
SEQ ID NO.295:aaauuagaaggcuaugccuucgaacauaucguuuauggagauuuuagucauagucag uuaggugguuuacaucuacugauuggacuagcuaaacguuuua
SEQ ID NO.296:aggaaucaccuuuugaauuagaagauuuuauuccuauggacaguacaguuaaaaacua uuucauaacagaugcgcaaacagguucaucuaagugugugug
SEQ ID NO.297:guucuguuauugauuuauuacuugaugauuuuguugaaauaauaaaaucccaagauu uaucuguaguuucuaagguugucaaagugacuauugacuauac
SEQ ID NO.298:cagaaauuucauuuaugcuuugguguaaagauggccauguagaaacauuuuacccaaa auuacaaucuagucaagcguggcaaccggguguugcuaugcc
SEQ ID NO.299:ccuaaucuuuacaaaaugcaaagaaugcuauuagaaaagugugaccuucaaaauuaug gugauagugcaacauuaccuaaaggcauaaugaugaaugucg
SEQ ID NO.300:gcaaaauauacucaacugugucaauauuuaaacacauuaacauuagcuguacccuaua auaugagaguuauacauuuuggugcugguucugauaaaggag
SEQ ID NO.301:guugcaccagguacagcuguuuuaagacagugguugccuacggguacgcugcuuguc gauucagaucuuaaugacuuugucucugaugcagauucaacuu
SEQ ID NO.302:uugauuggugauugugcaacuguacauacagcuaauaaaugggaucucauuauuagu gauauguacgacccuaagacuaaaaauguuacaaaagaaaaug
SEQ ID NO.303:auugaugcaaaagagggcgguaauuuuaacuaucuuuaccgucucuuuagaaaagcu aaucuuaaacccuuugagagggauaucucaacugaaauuuacc
SEQ ID NO.304:caagcaggcagcaaaccuuguaauggucaaacuggucuaaauugcuacuacccacuuu auagauauggauuuuacccuacugaugguguuggucaccaac
SEQ ID NO.305:caauuucaacuuuaaugguuuaacuggcacagguguccucacagagucuaauaaaaag uuucuaccuuuccaacaauuugguagagacauugcagacacu
SEQ ID NO.306:ugauccuuugcaaccagaacuugauucauucaaggaggaguuggauaaauacuuuaaa aaucauacaucaccugauguagauuuaggugacauuucuggc
SEQ ID NO.307:acuucacaguggcuaacaaauaucuuuggcacugucuaugagcaacucaaaccuguuc uugauuggcucgaagagaaauucaaggaagguguagaguuuc
SEQ ID NO.308:acauuugucacacacucaaagggauuguauagaaaguguguuaaacccaaagaagaaa cuggcuuacucaugccucugaaagcuccaaaagaaauuauuu
SEQ ID NO.309:gauagucaacaaacugucguucaagaagaugacagugaaguuaaucagacaacuauca cucaaucaauugcugagguucaaccucaguuagagauggaac
SEQ ID NO.310:gccacugcauuauuaacacuacagcaaauagaguugaaauuuaacccaccugcuuuac aagaugccuacuacagggcaagagcuggugaagcugcuaauu
SEQ ID NO.311:ccaucagguaaaguugaggguugcaugguacaaguuacuugugguacaaccacacuu aauggucuuuggcuugaugauguagucuacuguccaagacaug
SEQ ID NO.312:aatgatacggcgaccaccgagatct
SEQ ID NO.313:caagcagaagacggcatacgagat
SEQ ID NO.314:AATGATACGGCGACCACCGAGATCTACACCGGTTGTTACACTCTTTCCCTACACGAC
SEQ ID NO.315:AATGATACGGCGACCACCGAGATCTACACGTGCTTACACACTCTTTCCCTACACGAC
SEQ ID NO.316:AATGATACGGCGACCACCGAGATCTACACTCGTGGATACACTCTTTCCCTACACGAC
SEQ ID NO.317:AATGATACGGCGACCACCGAGATCTACACGTGAAGTGACACTCTTTCCCTACACGAC
SEQ ID NO.318:AATGATACGGCGACCACCGAGATCTACACTTGGTCTCACACTCTTTCCCTACACGAC
SEQ ID NO.319:AATGATACGGCGACCACCGAGATCTACACGTGCCATAACACTCTTTCCCTACACGAC
SEQ ID NO.320:AATGATACGGCGACCACCGAGATCTACACCTACAGTGACACTCTTTCCCTACACGAC
SEQ ID NO.321:AATGATACGGCGACCACCGAGATCTACACCCAGTGTTACACTCTTTCCCTACACGAC
SEQ ID NO.322:AATGATACGGCGACCACCGAGATCTACACTTACCGAGACACTCTTTCCCTACACGAC
SEQ ID NO.323:AATGATACGGCGACCACCGAGATCTACACGATTACCGACACTCTTTCCCTACACGAC
SEQ ID NO.324:CAAGCAGAAGACGGCATACGAGATATCGATCGGTGACTG GAGTTCAGACGTGT
SEQ ID NO.325:CAAGCAGAAGACGGCATACGAGATCGGTCATAGTGACTG GAGTTCAGACGTGT
SEQ ID NO.326:CAAGCAGAAGACGGCATACGAGATAGTCGCTTGTGACTG GAGTTCAGACGTGT
SEQ ID NO.327:CAAGCAGAAGACGGCATACGAGATACAGCAACGTGACTG GAGTTCAGACGTGT
SEQ ID NO.328:CAAGCAGAAGACGGCATACGAGATGTGCTTACGTGACTG GAGTTCAGACGTGT
SEQ ID NO.329:CAAGCAGAAGACGGCATACGAGATCCTCAGTTGTGACTG GAGTTCAGACGTGT
SEQ ID NO.330:CAAGCAGAAGACGGCATACGAGATATTCGAGGGTGACTG GAGTTCAGACGTGT
SEQ ID NO.331:CAAGCAGAAGACGGCATACGAGATAACTGGTGGTGACTG GAGTTCAGACGTGT
SEQ ID NO.332:CAAGCAGAAGACGGCATACGAGATAACCTCCTGTGACTG GAGTTCAGACGTGT
SEQ ID NO.333:CAAGCAGAAGACGGCATACGAGATAAGAGCCAGTGACTG GAGTTCAGACGTGT
SEQ ID NO.334:ACACTCTTTCCCTACACGACGCTCTTCCGATCTNNNT
SEQ ID NO.335:NNNAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC。
序列表
<110> 广西识远医学检验实验室有限公司
<120> 一种新型β冠状病毒变异检测方法、探针和试剂盒
<140> 2020101151428
<141> 2020-02-25
<160> 335
<170> SIPOSequenceListing 1.0
<210> 1
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 1
aucuaaguca cuaacugaga auaaauauuc ucaauuagau gaagagcaac caauggagau 60
ugauuaaacg aacaugaaaa uuauucuuuu cuuggcacug 100
<210> 2
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 2
gauaacacuc gcuacuugug agcuuuauca cuaccaagag uguguuagag guacaacagu 60
acuuuuaaaa gaaccuugcu cuucuggaac auacgagggc 100
<210> 3
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 3
gggcaauuca ccauuucauc cucuagcuga uaacaaauuu gcacugacuu gcuuuagcac 60
ucaauuugcu uuugcuuguc cugacggcgu aaaacacguc 100
<210> 4
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 4
ucaguuacgu gccagaucag uuucaccuaa acuguucauc agacaagagg aaguucaaga 60
acuuuacucu ccaauuuuuc uuauuguugc ggcaauagug 100
<210> 5
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 5
guuuauaaca cuuugcuuca cacucaaaag aaagacagaa ugauugaacu uucauuaauu 60
gacuucuauu ugugcuuuuu agccuuucug cuauuccuug 100
<210> 6
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 6
guuuuaauua ugcuuauuau cuuuugguuc ucacuugaac ugcaagauca uaaugaaacu 60
ugucacgccu aaacgaacau gaaauuucuu guuuucuuag 100
<210> 7
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 7
ggaaucauca caacuguagc ugcauuucac caagaaugua guuuacaguc auguacucaa 60
caucaaccau auguaguuga ugacccgugu ccuauucacu 100
<210> 8
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 8
cuucuauucu aaaugguaua uuagaguagg agcuagaaaa ucagcaccuu uaauugaauu 60
gugcguggau gaggcugguu cuaaaucacc cauucaguac 100
<210> 9
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 9
aucgauaucg guaauuauac aguuuccugu uuaccuuuua caauuaauug ccaggaaccu 60
aaauugggua gucuuguagu gcguuguucg uucuaugaag 100
<210> 10
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 10
gacuuuuuag aguaucauga cguucguguu guuuuagauu ucaucuaaac gaacaaacua 60
aaaugucuga uaauggaccc caaaaucagc gaaaugcacc 100
<210> 11
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 11
cccgcauuac guuuggugga cccucagauu caacuggcag uaaccagaau ggagaacgca 60
guggggcgcg aucaaaacaa cgucggcccc aagguuuacc 100
<210> 12
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 12
ccaauaauac ugcgucuugg uucaccgcuc ucacucaaca uggcaaggaa gaccuuaaau 60
ucccucgagg acaaggcguu ccaauuaaca ccaauagcag 100
<210> 13
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 13
guccagauga ccaaauuggc uacuaccgaa gagcuaccag acgaauucgu gguggugacg 60
guaaaaugaa agaucucagu ccaagauggu auuucuacua 100
<210> 14
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 14
ccuaggaacu gggccagaag cuggacuucc cuauggugcu aacaaagacg gcaucauaug 60
gguugcaacu gagggagccu ugaauacacc aaaagaucac 100
<210> 15
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 15
acauuggcac ccgcaauccu gcuaacaaug cugcaaucgu gcuacaacuu ccucaaggaa 60
caacauugcc aaaaggcuuc uacgcagaag ggagcagagg 100
<210> 16
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 16
cggcagucaa gccucuucuc guuccucauc acguagucgc aacaguucaa gaaauucaac 60
uccaggcagc aguaggggaa cuucuccugc uagaauggcu 100
<210> 17
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 17
cuggcaaugg cggugaugcu gcucuugcuu ugcugcugcu ugacagauug aaccagcuug 60
agagcaaaau gucugguaaa ggccaacaac aacaaggcca 100
<210> 18
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 18
aaacugucac uaagaaaucu gcugcugagg cuucuaagaa gccucggcaa aaacguacug 60
ccacuaaagc auacaaugua acacaagcuu ucggcagacg 100
<210> 19
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 19
gugguccaga acaaacccaa ggaaauuuug gggaccagga acuaaucaga caaggaacug 60
auuacaaaca uuggccgcaa auugcacaau uugcccccag 100
<210> 20
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 20
cgcuucagcg uucuucggaa ugucgcgcau uggcauggaa gucacaccuu cgggaacgug 60
guugaccuac acaggugcca ucaaauugga ugacaaagau 100
<210> 21
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 21
auccaaauuu caaagaucaa gucauuuugc ugaauaagca uauugacgca uacaaaacau 60
ucccaccaac agagccuaaa aaggacaaaa agaagaaggc 100
<210> 22
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 22
gcugaugaaa cucaagccuu accgcagaga cagaagaaac agcaaacugu gacucuucuu 60
ccugcugcag auuuggauga uuucuccaaa caauugcaac 100
<210> 23
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 23
caauccauga gcagugcuga cucaacucag gccuaaacuc augcagacca cacaaggcag 60
augggcuaua uaaacguuuu cgcuuuuccg uuuacgauau 100
<210> 24
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 24
auagucuacu cuugugcaga augaauucuc guaacuacau agcacaagua gauguaguua 60
acuuuaaucu cacauagcaa ucuuuaauca guguguaaca 100
<210> 25
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 25
auuagggagg acuugaaaga gccaccacau uuucaccgag gccacgcgga guacgaucga 60
guguacagug aacaaugcua gggagagcug ccuauaugga 100
<210> 26
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 26
uauggaagag cccuaaugug uaaaauuaau uuuaguagug cuauccccau gugauuuuaa 60
uagcuucuua ggagaaugac aaaaaaaaaa aaaaaaaaaa 100
<210> 27
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 27
cucuaaagag gguuuuuuca cuuacauuug uggguuuaua caacaaaagc uagcucuugg 60
agguuccgug gcuauaaaga uaacagaaca uucuuggaau 100
<210> 28
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 28
ugcugaucuu uauaagcuca ugggacacuu cgcauggugg acagccuuug uuacuaaugu 60
gaaugcguca ucaucugaag cauuuuuaau uggauguaau 100
<210> 29
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 29
uuaucuuggc aaaccacgcg aacaaauaga ugguuauguc augcaugcaa auuacauauu 60
uuggaggaau acaaauccaa uucaguuguc uuccuauucu 100
<210> 30
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 30
cuuuauuuga caugaguaaa uuuccccuua aauuaagggg uacugcuguu augucuuuaa 60
aagaagguca aaucaaugau augauuuuau cucuucuuag 100
<210> 31
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 31
agguagacuu auaauuagag aaaacaacag aguuguuauu ucuagugaug uucuuguuaa 60
caacuaaacg aacaauguuu guuuuucuug uuuuauugcc 100
<210> 32
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 32
ugccacuagu cucuagucag uguguuaauc uuacaaccag aacucaauua cccccugcau 60
acacuaauuc uuucacacgu gguguuuauu acccugacaa 100
<210> 33
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 33
aaguuuucag auccucaguu uuacauucaa cucaggacuu guucuuaccu uucuuuucca 60
auguuacuug guuccaugcu auacaugucu cugggaccaa 100
<210> 34
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 34
augguacuaa gagguuugau aacccugucc uaccauuuaa ugaugguguu uauuuugcuu 60
ccacugagaa gucuaacaua auaagaggcu ggauuuuugg 100
<210> 35
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 35
gguacuacuu uagauucgaa gacccagucc cuacuuauug uuaauaacgc uacuaauguu 60
guuauuaaag ucugugaauu ucaauuuugu aaugauccau 100
<210> 36
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 36
uggguguuua uuaccacaaa aacaacaaaa guuggaugga aagugaguuc agaguuuauu 60
cuagugcgaa uaauugcacu uuugaauaug ucucucagcc 100
<210> 37
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 37
cagccuuuuc uuauggaccu ugaaggaaaa caggguaauu ucaaaaaucu uagggaauuu 60
guguuuaaga auauugaugg uuauuuuaaa auauauucua 100
<210> 38
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 38
agcacacgcc uauuaauuua gugcgugauc ucccucaggg uuuuucggcu uuagaaccau 60
ugguagauuu gccaauaggu auuaacauca cuagguuuca 100
<210> 39
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 39
aaacuuuacu ugcuuuacau agaaguuauu ugacuccugg ugauucuucu ucagguugga 60
cagcuggugc ugcagcuuau uauguggguu aucuucaacc 100
<210> 40
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 40
cuaggacuuu ucuauuaaaa uauaaugaaa auggaaccau uacagaugcu guagacugug 60
cacuugaccc ucucucagaa acaaagugua cguugaaauc 100
<210> 41
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 41
ccuucacugu agaaaaagga aucuaucaaa cuucuaacuu uagaguccaa ccaacagaau 60
cuauuguuag auuuccuaau auuacaaacu ugugcccuuu 100
<210> 42
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 42
ggugaaguuu uuaacgccac cagauuugca ucuguuuaug cuuggaacag gaagagaauc 60
agcaacugug uugcugauua uucuguccua uauaauuccg 100
<210> 43
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 43
ccgcaucauu uuccacuuuu aaguguuaug gagugucucc uacuaaauua aaugaucucu 60
gcuuuacuaa ugucuaugca gauucauuug uaauuagagg 100
<210> 44
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 44
ggugaugaag ucagacaaau cgcuccaggg caaacuggaa agauugcuga uuauaauuau 60
aaauuaccag augauuuuac aggcugcguu auagcuugga 100
<210> 45
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 45
auucuaacaa ucuugauucu aagguuggug guaauuauaa uuaccuguau agauuguuua 60
ggaagucuaa ucucaaaccu uuugagagag auauuucaac 100
<210> 46
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 46
cugaaaucua ucaggccggu agcacaccuu guaauggugu ugaagguuuu aauuguuacu 60
uuccuuuaca aucauauggu uuccaaccca cuaauggugu 100
<210> 47
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 47
guugguuacc aaccauacag aguaguagua cuuucuuuug aacuucuaca ugcaccagca 60
acuguuugug gaccuaaaaa gucuacuaau uugguuaaaa 100
<210> 48
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 48
acaaaugugu caauuucaac uucaaugguu uaacaggcac agguguucuu acugagucua 60
acaaaaaguu ucugccuuuc caacaauuug gcagagacau 100
<210> 49
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 49
uugcugacac uacugaugcu guccgugauc cacagacacu ugagauucuu gacauuacac 60
cauguucuuu uggugguguc aguguuauaa caccaggaac 100
<210> 50
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 50
caaauacuuc uaaccagguu gcuguucuuu aucaggaugu uaacugcaca gaagucccug 60
uugcuauuca ugcagaucaa cuuacuccua cuuggcgugu 100
<210> 51
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 51
uuuauucuac agguucuaau guuuuucaaa cacgugcagg cuguuuaaua ggggcugaac 60
augucaacaa cucauaugag ugugacauac ccauuggugc 100
<210> 52
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 52
cagguauaug cgcuaguuau cagacucaga cuaauucucc ucggcgggca cguaguguag 60
cuagucaauc caucauugcc uacacuaugu cacuuggugc 100
<210> 53
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 53
cagaaaauuc aguugcuuac ucuaauaacu cuauugccau acccacaaau uuuacuauua 60
guguuaccac agaaauucua ccagugucua ugaccaagac 100
<210> 54
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 54
caucaguaga uuguacaaug uacauuugug gugauucaac ugaaugcagc aaucuuuugu 60
ugcaauaugg caguuuuugu acacaauuaa accgugcuuu 100
<210> 55
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 55
uaacuggaau agcuguugaa caagacaaaa acacccaaga aguuuuugca caagucaaac 60
aaauuuacaa aacaccacca auuaaagauu uuggugguuu 100
<210> 56
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 56
uaauuuuuca caaauauuac cagauccauc aaaaccaagc aagaggucau uuauugaaga 60
ucuacuuuuc aacaaaguga cacuugcaga ugcuggcuuc 100
<210> 57
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 57
ucaucaaaca auauggugau ugccuuggug auauugcugc uagagaccuc auuugugcac 60
aaaaguuuaa cggccuuacu guuuugccac cuuugcucac 100
<210> 58
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 58
cagaugaaau gauugcucaa uacacuucug cacuguuagc ggguacaauc acuucugguu 60
ggaccuuugg ugcaggugcu gcauuacaaa uaccauuugc 100
<210> 59
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 59
cuaugcaaau ggcuuauagg uuuaauggua uuggaguuac acagaauguu cucuaugaga 60
accaaaaauu gauugccaac caauuuaaua gugcuauugg 100
<210> 60
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 60
ggcaaaauuc aagacucacu uucuuccaca gcaagugcac uuggaaaacu ucaagaugug 60
gucaaccaaa augcacaagc uuuaaacacg cuuguuaaac 100
<210> 61
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 61
aacuuagcuc caauuuuggu gcaauuucaa guguuuuaaa ugauauccuu ucacgucuug 60
acaaaguuga ggcugaagug caaauugaua gguugaucac 100
<210> 62
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 62
caggcagacu ucaaaguuug cagacauaug ugacucaaca auuaauuaga gcugcagaaa 60
ucagagcuuc ugcuaaucuu gcugcuacua aaaugucaga 100
<210> 63
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 63
gaguguguac uuggacaauc aaaaagaguu gauuuuugug gaaagggcua ucaucuuaug 60
uccuucccuc agucagcacc ucauggugua gucuucuugc 100
<210> 64
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 64
augugacuua ugucccugca caagaaaaga acuucacaac ugcuccugcc auuugucaug 60
auggaaaagc acacuuuccu cgugaaggug ucuuuguuuc 100
<210> 65
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 65
caaauggcac acacugguuu guaacacaaa ggaauuuuua ugaaccacaa aucauuacua 60
cagacaacac auuugugucu gguaacugug auguuguaau 100
<210> 66
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 66
uaggaauugu caacaacaca guuuaugauc cuuugcaacc ugaauuagac ucauucaagg 60
aggaguuaga uaaauauuuu aagaaucaua caucaccaga 100
<210> 67
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 67
auguugauuu aggugacauc ucuggcauua augcuucagu uguaaacauu caaaaagaaa 60
uugaccgccu caaugagguu gccaagaauu uaaaugaauc 100
<210> 68
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 68
cucucaucga ucuccaagaa cuuggaaagu augagcagua uauaaaaugg ccaugguaca 60
uuuggcuagg uuuuauagcu ggcuugauug ccauaguaau 100
<210> 69
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 69
ggugacaauu augcuuugcu guaugaccag uugcuguagu ugucucaagg gcuguuguuc 60
uuguggaucc ugcugcaaau uugaugaaga cgacucugag 100
<210> 70
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 70
gagccagugc ucaaaggagu caaauuacau uacacauaaa cgaacuuaug gauuuguuua 60
ugagaaucuu cacaauugga acuguaacuu ugaagcaagg 100
<210> 71
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 71
gugaaaucaa ggaugcuacu ccuucagauu uuguucgcgc uacugcaacg auaccgauac 60
aagccucacu cccuuucgga uggcuuauug uuggcguugc 100
<210> 72
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 72
acuucuugcu guuuuucaga gcgcuuccaa aaucauaacc cucaaaaaga gauggcaacu 60
agcacucucc aaggguguuc acuuuguuug caacuugcug 100
<210> 73
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 73
guuguuguuu guaacaguuu acucacaccu uuugcucguu gcugcuggcc uugaagcccc 60
uuuucucuau cuuuaugcuu uagucuacuu cuugcagagu 100
<210> 74
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 74
guauaaacuu uguaagaaua auaaugaggc uuuggcuuug cuggaaaugc cguuccaaaa 60
acccauuacu uuaugaugcc aacuauuuuc uuugcuggca 100
<210> 75
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 75
uacuaauugu uacgacuauu guauaccuua caauagugua acuucuucaa uugucauuac 60
uucaggugau ggcacaacaa guccuauuuc ugaacaugac 100
<210> 76
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 76
cuaccagauu ggugguuaua cugaaaaaug ggaaucugga guaaaagacu guguuguauu 60
acacaguuac uucacuucag acuauuacca gcuguacuca 100
<210> 77
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 77
acucaauuga guacagacac ugguguugaa cauguuaccu ucuucaucua caauaaaauu 60
guugaugagc cugaagaaca uguccaaauu cacacaaucg 100
<210> 78
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 78
cgacgguuca uccggaguug uuaauccagu aauggaacca auuuaugaug aaccgacgac 60
gacuacuagc gugccuuugu aagcacaagc ugaugaguac 100
<210> 79
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 79
cgaacuuaug uacucauucg uuucggaaga gacagguacg uuaauaguua auagcguacu 60
ucuuuuucuu gcuuucgugg uauucuugcu aguuacacua 100
<210> 80
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 80
gccauccuua cugcgcuucg auugugugcg uacugcugca auauuguuaa cgugagucuu 60
guaaaaccuu cuuuuuacgu uuacucucgu guuaaaaauc 100
<210> 81
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 81
ucugaauucu ucuagaguuc cugaucuucu ggucuaaacg aacuaaauau uauauuaguu 60
uuucuguuug gaacuuuaau uuuagccaug gcagauucca 100
<210> 82
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 82
cgguacuauu accguugaag agcuuaaaaa gcuccuugaa caauggaacc uaguaauagg 60
uuuccuauuc cuuacaugga uuugucuucu acaauuugcc 100
<210> 83
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 83
gccuaugcca acaggaauag guuuuuguau auaauuaagu uaauuuuccu cuggcuguua 60
uggccaguaa cuuuagcuug uuuugugcuu gcugcuguuu 100
<210> 84
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 84
cagaauaaau uggaucaccg guggaauugc uaucgcaaug gcuugucuug uaggcuugau 60
guggcucagc uacuucauug cuucuuucag acuguuugcg 100
<210> 85
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 85
gcgcguacgc guuccaugug gucauucaau ccagaaacua acauucuucu caacgugcca 60
cuccauggca cuauucugac cagaccgcuu cuagaaagug 100
<210> 86
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 86
gaacucguaa ucggagcugu gauccuucgu ggacaucuuc guauugcugg acaccaucua 60
ggacgcugug acaucaagga ccugccuaaa gaaaucacug 100
<210> 87
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 87
guugcuacau cacgaacgcu uucuuauuac aaauugggag cuucgcagcg uguagcaggu 60
gacucagguu uugcugcaua cagucgcuac aggauuggca 100
<210> 88
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 88
gcaacuauaa auuaaacaca gaccauucca guagcaguga caauauugcu uugcuuguac 60
aguaagugac aacagauguu ucaucucguu gacuuucagg 100
<210> 89
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 89
uacuauagca gagauauuac uaauuauuau gaggacuuuu aaaguuucca uuuggaaucu 60
ugauuacauc auaaaccuca uaauuaaaaa uuuaucuaag 100
<210> 90
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 90
aaagguuuau accuucccag guaacaaacc aaccaacuuu cgaucucuug uagaucuguu 60
cucuaaacga acuuuaaaau cuguguggcu gucacucggc 100
<210> 91
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 91
cggcugcaug cuuagugcac ucacgcagua uaauuaauaa cuaauuacug ucguugacag 60
gacacgagua acucgucuau cuucugcagg cugcuuacgg 100
<210> 92
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 92
uuucguccgu guugcagccg aucaucagca caucuagguu ucguccgggu gugaccgaaa 60
gguaagaugg agagccuugu cccugguuuc aacgagaaaa 100
<210> 93
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 93
aacacacguc caacucaguu ugccuguuuu acagguucgc gacgugcucg uacguggcuu 60
uggagacucc guggaggagg ucuuaucaga ggcacgucaa 100
<210> 94
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 94
acaucuuaaa gauggcacuu guggcuuagu agaaguugaa aaaggcguuu ugccucaacu 60
ugaacagccc uauguguuca ucaaacguuc ggaugcucga 100
<210> 95
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 95
aacugcaccu cauggucaug uuaugguuga gcugguagca gaacucgaag gcauucagua 60
cggucguagu ggugagacac uugguguccu ugucccucau 100
<210> 96
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 96
gugggcgaaa uaccaguggc uuaccgcaag guucuucuuc guaagaacgg uaauaaagga 60
gcugguggcc auaguuacgg cgccgaucua aagucauuug 100
<210> 97
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 97
gacuuaggcg acgagcuugg cacugauccu uaugaagauu uucaagaaaa cuggaacacu 60
aaacauagca gugguguuac ccgugaacuc augcgugagc 100
<210> 98
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 98
cuuaacggag gggcauacac ucgcuauguc gauaacaacu ucuguggccc ugauggcuac 60
ccucuugagu gcauuaaaga ccuucuagca cgugcuggua 100
<210> 99
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 99
aaagcuucau gcacuuuguc cgaacaacug gacuuuauug acacuaagag ggguguauac 60
ugcugccgug aacaugagca ugaaauugcu ugguacacgg 100
<210> 100
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 100
cggaacguuc ugaaaagagc uaugaauugc agacaccuuu ugaaauuaaa uuggcaaaga 60
aauuugacac cuucaauggg gaauguccaa auuuuguauu 100
<210> 101
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 101
cccuuaaauu ccauaaucaa gacuauucaa ccaaggguug aaaagaaaaa gcuugauggc 60
uuuaugggua gaauucgauc ugucuaucca guugcgucac 100
<210> 102
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 102
ccaaaugaau gcaaccaaau gugccuuuca acucucauga agugugauca uuguggugaa 60
acuucauggc agacgggcga uuuuguuaaa gccacuugcg 100
<210> 103
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 103
cgaauuuugu ggcacugaga auuugacuaa agaaggugcc acuacuugug guuacuuacc 60
ccaaaaugcu guuguuaaaa uuuauugucc agcaugucac 100
<210> 104
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 104
caauucagaa guaggaccug agcauagucu ugccgaauac cauaaugaau cuggcuugaa 60
aaccauucuu cguaagggug gucgcacuau ugccuuugga 100
<210> 105
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 105
ggcugugugu ucucuuaugu ugguugccau aacaagugug ccuauugggu uccacgugcu 60
agcgcuaaca uagguuguaa ccauacaggu guuguuggag 100
<210> 106
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 106
gaagguuccg aaggucuuaa ugacaaccuu cuugaaauac uccaaaaaga gaaagucaac 60
aucaauauug uuggugacuu uaaacuuaau gaagagaucg 100
<210> 107
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 107
cgccauuauu uuggcaucuu uuucugcuuc cacaagugcu uuuguggaaa cugugaaagg 60
uuuggauuau aaagcauuca aacaaauugu ugaauccugu 100
<210> 108
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 108
gguaauuuua aaguuacaaa aggaaaagcu aaaaaaggug ccuggaauau uggugaacag 60
aaaucaauac ugaguccucu uuaugcauuu gcaucagagg 100
<210> 109
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 109
ggcugcucgu guuguacgau caauuuucuc ccgcacucuu gaaacugcuc aaaauucugu 60
gcguguuuua cagaaggccg cuauaacaau acuagaugga 100
<210> 110
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 110
ggaauuucac aguauucacu gagacucauu gaugcuauga uguucacauc ugauuuggcu 60
acuaacaauc uaguuguaau ggccuacauu acagguggug 100
<210> 111
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 111
guucaguuga cuucgcagug gcuaacuaac aucuuuggca cuguuuauga aaaacucaaa 60
cccguccuug auuggcuuga agagaaguuu aaggaaggug 100
<210> 112
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 112
gguguagagu uucuuagaga cgguugggaa auuguuaaau uuaucucaac cugugcuugu 60
gaaauugucg guggacaaau ugucaccugu gcaaaggaaa 100
<210> 113
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 113
uaaggagagu guucagacau ucuuuaagcu uguaaauaaa uuuuuggcuu ugugugcuga 60
cucuaucauu auugguggag cuaaacuuaa agccuugaau 100
<210> 114
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 114
uuaggugaaa cauuugucac gcacucaaag ggauuguaca gaaagugugu uaaauccaga 60
gaagaaacug gccuacucau gccucuaaaa gccccaaaag 100
<210> 115
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 115
agaaauuauc uucuuagagg gagaaacacu ucccacagaa guguuaacag aggaaguugu 60
cuugaaaacu ggugauuuac aaccauuaga acaaccuacu 100
<210> 116
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 116
gugaagcugu ugaagcucca uugguuggua caccaguuug uauuaacggg cuuauguugc 60
ucgaaaucaa agacacagaa aaguacugug cccuugcacc 100
<210> 117
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 117
accuaauaug augguaacaa acaauaccuu cacacucaaa ggcggugcac caacaaaggu 60
uacuuuuggu gaugacacug ugauagaagu gcaagguuac 100
<210> 118
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 118
caagagugug aauaucacuu uugaacuuga ugaaaggauu gauaaaguac uuaaugagaa 60
gugcucugcc uauacaguug aacucgguac agaaguaaau 100
<210> 119
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 119
gaguucgccu guguuguggc agaugcuguc auaaaaacuu ugcaaccagu aucugaauua 60
cuuacaccac ugggcauuga uuuagaugag uggaguaugg 100
<210> 120
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 120
ggcuacauac uacuuauuug augagucugg ugaguuuaaa uuggcuucac auauguauug 60
uucuuucuac ccuccagaug aggaugaaga agaaggugau 100
<210> 121
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 121
ugugaagaag aagaguuuga gccaucaacu caauaugagu augguacuga agaugauuac 60
caagguaaac cuuuggaauu uggugccacu ucugcugcuc 100
<210> 122
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 122
cucuucaacc ugaagaagag caagaagaag auugguuaga ugaugauagu caacaaacug 60
uuggucaaca agacggcagu gaggacaauc agacaacuac 100
<210> 123
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 123
cuauucaaac aauuguugag guucaaccuc aauuagagau ggaacuuaca ccaguuguuc 60
agacuauuga agugaauagu uuuagugguu auuuaaaacu 100
<210> 124
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 124
cugacaaugu auacauuaaa aaugcagaca uuguggaaga agcuaaaaag guaaaaccaa 60
cagugguugu uaaugcagcc aauguuuacc uuaaacaugg 100
<210> 125
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 125
ggaggaggug uugcaggagc cuuaaauaag gcuacuaaca augccaugca aguugaaucu 60
gaugauuaca uagcuacuaa uggaccacuu aaagugggug 100
<210> 126
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 126
gugguaguug uguuuuaagc ggacacaauc uugcuaaaca cugucuucau guugucggcc 60
caaauguuaa caaaggugaa gacauucaac uucuuaagag 100
<210> 127
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 127
gugcuuauga aaauuuuaau cagcacgaag uucuacuugc accauuauua ucagcuggua 60
uuuuuggugc ugacccuaua cauucuuuaa gaguuugugu 100
<210> 128
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 128
gauacuguuc gcacaaaugu cuacuuagcu gucuuugaua aaaaucucua ugacaaacuu 60
guuucaagcu uuuuggaaau gaagagugaa aagcaaguug 100
<210> 129
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 129
ugaacaaaag aucgcugaga uuccuaaaga ggaaguuaag ccauuuauaa cugaaaguaa 60
accuucaguu gaacagagaa aacaagauga uaagaaaauc 100
<210> 130
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 130
caaagcuugu guugaagaag uuacaacaac ucuggaagaa acuaaguucc ucacagaaaa 60
cuuguuacuu uauauugaca uuaauggcaa ucuucaucca 100
<210> 131
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 131
cagauucugc cacucuuguu agugacauug acaucacuuu cuuaaagaaa gaugcuccau 60
auauaguggg ugauguuguu caagagggug uuuuaacugc 100
<210> 132
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 132
cugugguuau accuacuaaa aaggcuggug gcacuacuga aaugcuagcg aaagcuuuga 60
gaaaagugcc aacagacaau uauauaacca cuuacccggg 100
<210> 133
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 133
caggguuuaa augguuacac uguagaggag gcaaagacag ugcuuaaaaa guguaaaagu 60
gccuuuuaca uucuaccauc uauuaucucu aaugagaagc 100
<210> 134
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 134
gcaagaaauu cuuggaacug uuucuuggaa uuugcgagaa augcuugcac augcagaaga 60
aacacgcaaa uuaaugccug ucugugugga aacuaaagcc 100
<210> 135
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 135
ccauaguuuc aacuauacag cguaaauaua aggguauuaa aauacaagag ggugugguug 60
auuauggugc uagauuuuac uuuuacacca guaaaacaac 100
<210> 136
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 136
cuguagcguc acuuaucaac acacuuaacg aucuaaauga aacucuuguu acaaugccac 60
uuggcuaugu aacacauggc uuaaauuugg aagaagcugc 100
<210> 137
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 137
cucgguauau gagaucucuc aaagugccag cuacaguuuc uguuucuuca ccugaugcug 60
uuacagcgua uaaugguuau cuuacuucuu cuucuaaaac 100
<210> 138
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 138
accugaagaa cauuuuauug aaaccaucuc acuugcuggu uccuauaaag auugguccua 60
uucuggacaa ucuacacaac uagguauaga auuucuuaag 100
<210> 139
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 139
gagaggugau aaaaguguau auuacacuag uaauccuacc acauuccacc uagaugguga 60
aguuaucacc uuugacaauc uuaagacacu ucuuucuuug 100
<210> 140
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 140
gagagaagug aggacuauua agguguuuac aacaguagac aacauuaacc uccacacgca 60
aguuguggac augucaauga cauauggaca acaguuuggu 100
<210> 141
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 141
guccaacuua uuuggaugga gcugauguua cuaaaauaaa accucauaau ucacaugaag 60
guaaaacauu uuauguuuua ccuaaugaug acacucuacg 100
<210> 142
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 142
cguguugagg cuuuugagua cuaccacaca acugauccua guuuucuggg uagguacaug 60
ucagcauuaa aucacacuaa aaaguggaaa uacccacaag 100
<210> 143
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 143
aaugguuuaa cuucuauuaa augggcagau aacaacuguu aucuugccac ugcauuguua 60
acacuccaac aaauagaguu gaaguuuaau ccaccugcuc 100
<210> 144
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 144
gcucuacaag augcuuauua cagagcaagg gcuggugaag cugcuaacuu uugugcacuu 60
aucuuagccu acuguaauaa gacaguaggu gaguuaggug 100
<210> 145
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 145
uguuagagaa acaaugaguu acuuguuuca acaugccaau uuagauucuu gcaaaagagu 60
cuugaacgug guguguaaaa cuuguggaca acagcagaca 100
<210> 146
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 146
caacccuuaa ggguguagaa gcuguuaugu acaugggcac acuuucuuau gaacaauuua 60
agaaaggugu ucagauaccu uguacgugug guaaacaagc 100
<210> 147
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 147
uacaaaauau cuaguacaac aggagucacc uuuuguuaug augucagcac caccugcuca 60
guaugaacuu aagcauggua cauuuacuug ugcuagugag 100
<210> 148
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 148
guacacuggu aauuaccagu guggucacua uaaacauaua acuucuaaag aaacuuugua 60
uugcauagac ggugcuuuac uuacaaaguc cucagaauac 100
<210> 149
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 149
caaagguccu auuacggaug uuuucuacaa agaaaacagu uacacaacaa ccauaaaacc 60
aguuacuuau aaauuggaug guguuguuug uacagaaauu 100
<210> 150
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 150
gacccuaagu uggacaauua uuauaagaaa gacaauucuu auuucacaga gcaaccaauu 60
gaucuuguac caaaccaacc auauccaaac gcaagcuucg 100
<210> 151
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 151
cgauaauuuu aaguuuguau gugauaauau caaauuugcu gaugauuuaa accaguuaac 60
ugguuauaag aaaccugcuu caagagagcu uaaaguuaca 100
<210> 152
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 152
cauuuuuccc ugacuuaaau ggugaugugg uggcuauuga uuauaaacac uacacacccu 60
cuuuuaagaa aggagcuaaa uuguuacaua aaccuauugu 100
<210> 153
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 153
uuggcauguu aacaaugcaa cuaauaaagc cacguauaaa ccaaauaccu gguguauacg 60
uugucuuugg agcacaaaac caguugaaac aucaaauucg 100
<210> 154
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 154
cguuugaugu acugaaguca gaggacgcgc agggaaugga uaaucuugcc ugcgaagauc 60
uaaaaccagu cucugaagaa guaguggaaa auccuaccau 100
<210> 155
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 155
cagaaagacg uucuugagug uaaugugaaa acuaccgaag uuguaggaga cauuauacuu 60
aaaccagcaa auaauaguuu aaaaauuaca gaagagguug 100
<210> 156
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 156
uggccacaca gaucuaaugg cugcuuaugu agacaauucu agucuuacua uuaagaaacc 60
uaaugaauua ucuagaguau uagguuugaa aacccuugcu 100
<210> 157
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 157
gcuacucaug guuuagcugc uguuaauagu gucccuuggg auacuauagc uaauuaugcu 60
aagccuuuuc uuaacaaagu uguuaguaca acuacuaaca 100
<210> 158
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 158
aguuacacgg uguuuaaacc guguuuguac uaauuauaug ccuuauuucu uuacuuuauu 60
gcuacaauug uguacuuuua cuagaaguac aaauucuaga 100
<210> 159
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 159
gaauuaaagc aucuaugccg acuacuauag caaagaauac uguuaagagu gucgguaaau 60
uuugucuaga ggcuucauuu aauuauuuga agucaccuaa 100
<210> 160
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 160
uuuuucuaaa cugauaaaua uuauaauuug guuuuuacua uuaaguguuu gccuagguuc 60
uuuaaccuac ucaaccgcug cuuuaggugu uuuaaugucu 100
<210> 161
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 161
cuaauuuagg caugccuucu uacuguacug guuacagaga aggcuauuug aacucuacua 60
augucacuau ugcaaccuac uguacugguu cuauaccuug 100
<210> 162
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 162
guguuugucu uagugguuua gauucuuuag acaccuaucc uucuuuagaa acuauacaaa 60
uuaccauuuc aucuuuuaaa ugggauuuaa cugcuuuugg 100
<210> 163
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 163
ggcuuaguug cagagugguu uuuggcauau auucuuuuca cuagguuuuu cuauguacuu 60
ggauuggcug caaucaugca auuguuuuuc agcuauuuug 100
<210> 164
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 164
gcaguacauu uuauuaguaa uucuuggcuu augugguuaa uaauuaaucu uguacaaaug 60
gccccgauuu cagcuauggu uagaauguac aucuucuuug 100
<210> 165
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 165
gcaucauuuu auuauguaug gaaaaguuau gugcauguug uagacgguug uaauucauca 60
acuuguauga uguguuacaa acguaauaga gcaacaagag 100
<210> 166
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 166
gagucgaaug uacaacuauu guuaauggug uuagaagguc cuuuuauguc uaugcuaaug 60
gagguaaagg cuuuugcaaa cuacacaauu ggaauugugu 100
<210> 167
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 167
uaauugugau acauucugug cugguaguac auuuauuagu gaugaaguug cgagagacuu 60
gucacuacag uuuaaaagac caauaaaucc uacugaccag 100
<210> 168
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 168
gucuucuuac aucguugaua guguuacagu gaagaauggu uccauccauc uuuacuuuga 60
uaaagcuggu caaaagacuu augaaagaca uucucucucu 100
<210> 169
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 169
cucauuuugu uaacuuagac aaccugagag cuaauaacac uaaagguuca uugccuauua 60
auguuauagu uuuugauggu aaaucaaaau gugaagaauc 100
<210> 170
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 170
ucugcaaaau cagcgucugu uuacuacagu cagcuuaugu gucaaccuau acuguuacua 60
gaucaggcau uagugucuga uguuggugau agugcggaag 100
<210> 171
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 171
guugcaguua aaauguuuga ugcuuacguu aauacguuuu caucaacuuu uaacguacca 60
auggaaaaac ucaaaacacu aguugcaacu gcagaagcug 100
<210> 172
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 172
gcugaacuug caaagaaugu guccuuagac aaugucuuau cuacuuuuau uucagcagcu 60
cggcaagggu uuguugauuc agauguagaa acuaaagaug 100
<210> 173
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 173
guugaauguc uuaaauuguc acaucaaucu gacauagaag uuacuggcga uaguuguaau 60
aacuauaugc ucaccuauaa caaaguugaa aacaugacac 100
<210> 174
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 174
caccccguga ccuuggugcu uguauugacu guagugcgcg ucauauuaau gcgcagguag 60
caaaaaguca caacauugcu uugauaugga acguuaaaga 100
<210> 175
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 175
uucaugucau ugucugaaca acuacgaaaa caaauacgua gugcugcuaa aaagaauaac 60
uuaccuuuua aguugacaug ugcaacuacu agacaaguug 100
<210> 176
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 176
guuaauguug uaacaacaaa gauagcacuu aaggguggua aaauuguuaa uaauugguug 60
aagcaguuaa uuaaaguuac acuuguguuc cuuuuuguug 100
<210> 177
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 177
ugcugcuauu uucuauuuaa uaacaccugu ucaugucaug ucuaaacaua cugacuuuuc 60
aagugaaauc auaggauaca aggcuauuga uggugguguc 100
<210> 178
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 178
cacucgugac auagcaucua cagauacuug uuuugcuaac aaacaugcug auuuugacac 60
augguuuagc cagcguggug guaguuauac uaaugacaaa 100
<210> 179
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 179
gcuugcccau ugauugcugc agucauaaca agagaagugg guuuugucgu gccugguuug 60
ccuggcacga uauuacgcac aacuaauggu gacuuuuugc 100
<210> 180
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 180
gcauuucuua ccuagaguuu uuagugcagu ugguaacauc uguuacacac caucaaaacu 60
uauagaguac acugacuuug caacaucagc uuguguuuug 100
<210> 181
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 181
ggcugcugaa uguacaauuu uuaaagaugc uucugguaag ccaguaccau auuguuauga 60
uaccaaugua cuagaagguu cuguugcuua ugaaaguuua 100
<210> 182
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 182
cgcccugaca cacguuaugu gcucauggau ggcucuauua uucaauuucc uaacaccuac 60
cuugaagguu cuguuagagu gguaacaacu uuugauucug 100
<210> 183
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 183
cugaguacug uaggcacggc acuugugaaa gaucagaagc ugguguuugu guaucuacua 60
gugguagaug gguacuuaac aaugauuauu acagaucuuu 100
<210> 184
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 184
ccaggaguuu ucuguggugu agaugcugua aauuuacuua cuaauauguu uacaccacua 60
auucaaccua uuggugcuuu ggacauauca gcaucuauag 100
<210> 185
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 185
aguagcuggu gguauuguag cuaucguagu aacaugccuu gccuacuauu uuaugagguu 60
uagaagagcu uuuggugaau acagucaugu aguugccuuu 100
<210> 186
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 186
uaauacuuua cuauuccuua ugucauucac uguacucugu uuaacaccag uuuacucauu 60
cuuaccuggu guuuauucug uuauuuacuu guacuugaca 100
<210> 187
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 187
cauuuuaucu uacuaaugau guuucuuuuu uagcacauau ucaguggaug guuauguuca 60
caccuuuagu accuuucugg auaacaauug cuuauaucau 100
<210> 188
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 188
uguauuucca caaagcauuu cuauugguuc uuuaguaauu accuaaagag acguguaguc 60
uuuaauggug uuuccuuuag uacuuuugaa gaagcugcgc 100
<210> 189
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 189
gcgcugugca ccuuuuuguu aaauaaagaa auguaucuaa aguugcguag ugaugugcua 60
uuaccucuua cgcaauauaa uagauacuua gcucuuuaua 100
<210> 190
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 190
uaaguacaag uauuuuagug gagcaaugga uacaacuagc uacagagaag cugcuuguug 60
ucaucucgca aaggcucuca augacuucag uaacucaggu 100
<210> 191
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 191
guucugaugu ucuuuaccaa ccaccacaaa ccucuaucac cucagcuguu uugcagagug 60
guuuuagaaa aauggcauuc ccaucuggua aaguugaggg 100
<210> 192
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 192
uuguauggua caaguaacuu gugguacaac uacacuuaac ggucuuuggc uugaugacgu 60
aguuuacugu ccaagacaug ugaucugcac cucugaagac 100
<210> 193
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 193
caugcuuaac ccuaauuaug aagauuuacu cauucguaag ucuaaucaua auuucuuggu 60
acaggcuggu aauguucaac ucaggguuau uggacauucu 100
<210> 194
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 194
uaugcaaaau uguguacuua agcuuaaggu ugauacagcc aauccuaaga caccuaagua 60
uaaguuuguu cgcauucaac caggacagac uuuuucagug 100
<210> 195
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 195
guuagcuugu uacaaugguu caccaucugg uguuuaccaa ugugcuauga ggcccaauuu 60
cacuauuaag gguucauucc uuaaugguuc augugguagu 100
<210> 196
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 196
uguugguuuu aacauagauu augacugugu cucuuuuugu uacaugcacc auauggaauu 60
accaacugga guucaugcug gcacagacuu agaagguaac 100
<210> 197
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 197
cuuuuaugga ccuuuuguug acaggcaaac agcacaagca gcugguacgg acacaacuau 60
uacaguuaau guuuuagcuu gguuguacgc ugcuguuaua 100
<210> 198
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 198
aauggagaca ggugguuucu caaucgauuu accacaacuc uuaaugacuu uaaccuugug 60
gcuaugaagu acaauuauga accucuaaca caagaccaug 100
<210> 199
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 199
uguugacaua cuaggaccuc uuucugcuca aacuggaauu gccguuuuag auaugugugc 60
uucauuaaaa gaauuacugc aaaaugguau gaauggacgu 100
<210> 200
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 200
guaccauauu ggguagugcu uuauuagaag augaauuuac accuuuugau guuguuagac 60
aaugcucagg uguuacuuuc caaagugcag ugaaaagaac 100
<210> 201
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 201
caaucaaggg uacacaccac ugguuguuac ucacaauuuu gacuucacuu uuaguuuuag 60
uccagaguac ucaauggucu uuguucuuuu uuuuguauga 100
<210> 202
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 202
augccuuuuu accuuuugcu auggguauua uugcuauguc ugcuuuugca augauguuug 60
ucaaacauaa gcaugcauuu cucuguuugu uuuuguuacc 100
<210> 203
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 203
accuucucuu gccacuguag cuuauuuuaa uauggucuau augccugcua guugggugau 60
gcguauuaug acaugguugg auaugguuga uacuaguuug 100
<210> 204
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 204
gucugguuuu aagcuaaaag acuguguuau guaugcauca gcuguagugu uacuaauccu 60
uaugacagca agaacugugu augaugaugg ugcuaggaga 100
<210> 205
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 205
gaguguggac acuuaugaau gucuugacac ucguuuauaa aguuuauuau gguaaugcuu 60
uagaucaagc cauuuccaug ugggcucuua uaaucucugu 100
<210> 206
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 206
uacuucuaac uacucaggug uaguuacaac ugucauguuu uuggccagag guauuguuuu 60
uauguguguu gaguauugcc cuauuuucuu cauaacuggu 100
<210> 207
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 207
uaauacacuu caguguauaa ugcuaguuua uuguuucuua ggcuauuuuu guacuuguua 60
cuuuggccuc uuuuguuuac ucaaccgcua cuuuagacug 100
<210> 208
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 208
gacucuuggu guuuaugauu acuuaguuuc uacacaggag uuuagauaua ugaauucaca 60
gggacuacuc ccacccaaga auagcauaga ugccuucaaa 100
<210> 209
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 209
cucaacauua aauuguuggg uguugguggc aaaccuugua ucaaaguagc cacuguacag 60
ucuaaaaugu cagauguaaa gugcacauca guagucuuac 100
<210> 210
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 210
cucucaguuu ugcaacaacu cagaguagaa ucaucaucua aauugugggc ucaauguguc 60
caguuacaca augacauucu cuuagcuaaa gauacuacug 100
<210> 211
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 211
gaagccuuug aaaaaauggu uucacuacuu ucuguuuugc uuuccaugca gggugcugua 60
gacauaaaca agcuuuguga agaaaugcug gacaacaggg 100
<210> 212
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 212
gggcaaccuu acaagcuaua gccucagagu uuaguucccu uccaucauau gcagcuuuug 60
cuacugcuca agaagcuuau gagcaggcug uugcuaaugg 100
<210> 213
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 213
gauucugaag uuguucuuaa aaaguugaag aagucuuuga auguggcuaa aucugaauuu 60
gaccgugaug cagccaugca acguaaguug gaaaagaugg 100
<210> 214
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 214
ggcugaucaa gcuaugaccc aaauguauaa acaggcuaga ucugaggaca agagggcaaa 60
aguuacuagu gcuaugcaga caaugcuuuu cacuaugcuu 100
<210> 215
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 215
uagaaaguug gauaaugaug cacucaacaa cauuaucaac aaugcaagag augguugugu 60
ucccuugaac auaauaccuc uuacaacagc agccaaacua 100
<210> 216
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 216
ugguugucau accagacuau aacacauaua aaaauacgug ugaugguaca acauuuacuu 60
augcaucagc auugugggaa auccaacagg uuguagaugc 100
<210> 217
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 217
gcagauagua aaauuguuca acuuagugaa auuaguaugg acaauucacc uaauuuagca 60
uggccucuua uuguaacagc uuuaagggcc aauucugcug 100
<210> 218
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 218
gcugucaaau uacagaauaa ugagcuuagu ccuguugcac uacgacagau gucuugugcu 60
gccgguacua cacaaacugc uugcacugau gacaaugcgu 100
<210> 219
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 219
gcuuacuaca acacaacaaa gggagguagg uuuguacuug cacuguuauc cgauuuacag 60
gauuugaaau gggcuagauu cccuaagagu gauggaacug 100
<210> 220
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 220
ugguacuauc uauacagaac uggaaccacc uuguagguuu guuacagaca caccuaaagg 60
uccuaaagug aaguauuuau acuuuauuaa aggauuaaac 100
<210> 221
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 221
caaccuaaau agagguaugg uacuugguag uuuagcugcc acaguacguc uacaagcugg 60
uaaugcaaca gaagugccug ccaauucaac uguauuaucu 100
<210> 222
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 222
uuucugugcu uuugcuguag augcugcuaa agcuuacaaa gauuaucuag cuaguggggg 60
acaaccaauc acuaauugug uuaagauguu guguacacac 100
<210> 223
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 223
cacugguacu ggucaggcaa uaacaguuac accggaagcc aauauggauc aagaauccuu 60
ugguggugca ucguguuguc uguacugccg uugccacaua 100
<210> 224
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 224
agaucaucca aauccuaaag gauuuuguga cuuaaaaggu aaguauguac aaauaccuac 60
aacuugugcu aaugacccug uggguuuuac acuuaaaaac 100
<210> 225
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 225
cacagucugu accgucugcg guauguggaa agguuauggc uguaguugug aucaacuccg 60
cgaacccaug cuucagucag cugaugcaca aucguuuuua 100
<210> 226
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 226
aaacggguuu gcgguguaag ugcagcccgu cuuacaccgu gcggcacagg cacuaguacu 60
gaugucguau acagggcuuu ugacaucuac aaugauaaag 100
<210> 227
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 227
guagcugguu uugcuaaauu ccuaaaaacu aauuguuguc gcuuccaaga aaaggacgaa 60
gaugacaauu uaauugauuc uuacuuugua guuaagagac 100
<210> 228
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 228
cacacuuucu cuaacuacca acaugaagaa acaauuuaua auuuacuuaa ggauugucca 60
gcuguugcua aacaugacuu cuuuaaguuu agaauagacg 100
<210> 229
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 229
cggugacaug guaccacaua uaucacguca acgucuuacu aaauacacaa uggcagaccu 60
cgucuaugcu uuaaggcauu uugaugaagg uaauugugac 100
<210> 230
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 230
acauuaaaag aaauacuugu cacauacaau uguugugaug augauuauuu caauaaaaag 60
gacugguaug auuuuguaga aaacccagau auauuacgcg 100
<210> 231
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 231
gcguauacgc caacuuaggu gaacguguac gccaagcuuu guuaaaaaca guacaauucu 60
gugaugccau gcgaaaugcu gguauuguug guguacugac 100
<210> 232
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 232
uuagauaauc aagaucucaa ugguaacugg uaugauuucg gugauuucau acaaaccacg 60
ccagguagug gaguuccugu uguagauucu uauuauucau 100
<210> 233
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 233
uuguuaaugc cuauauuaac cuugaccagg gcuuuaacug cagagucaca uguugacacu 60
gacuuaacaa agccuuacau uaagugggau uuguuaaaau 100
<210> 234
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 234
ugacuucacg gaagagaggu uaaaacucuu ugaccguuau uuuaaauauu gggaucagac 60
auaccaccca aauuguguua acuguuugga ugacagaugc 100
<210> 235
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 235
gcauucugca uugugcaaac uuuaauguuu uauucucuac aguguuccca ccuacaaguu 60
uuggaccacu agugagaaaa auauuuguug augguguucc 100
<210> 236
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 236
ccauuuguag uuucaacugg auaccacuuc agagagcuag guguuguaca uaaucaggau 60
guaaacuuac auagcucuag acuuaguuuu aaggaauuac 100
<210> 237
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 237
cuuguguaug cugcugaccc ugcuaugcac gcugcuucug guaaucuauu acuagauaaa 60
cgcacuacgu gcuuuucagu agcugcacuu acuaacaaug 100
<210> 238
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 238
guugcuuuuc aaacugucaa acccgguaau uuuaacaaag acuucuauga cuuugcugug 60
ucuaaggguu ucuuuaagga aggaaguucu guugaauuaa 100
<210> 239
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 239
aaacacuucu ucuuugcuca ggaugguaau gcugcuauca gcgauuauga cuacuaucgu 60
uauaaucuac caacaaugug ugauaucaga caacuacuau 100
<210> 240
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 240
uuuguaguug aaguuguuga uaaguacuuu gauuguuacg augguggcug uauuaaugcu 60
aaccaaguca ucgucaacaa ccuagacaaa ucagcugguu 100
<210> 241
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 241
uuccauuuaa uaaauggggu aaggcuagac uuuauuauga uucaaugagu uaugaggauc 60
aagaugcacu uuucgcauau acaaaacgua augucauccc 100
<210> 242
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 242
ccuacuauaa cucaaaugaa ucuuaaguau gccauuagug caaagaauag agcucgcacc 60
guagcuggug ucucuaucug uaguacuaug accaauagac 100
<210> 243
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 243
caguuucauc aaaaauuauu gaaaucaaua gccgccacua gaggagcuac uguaguaauu 60
ggaacaagca aauucuaugg ugguuggcac aacauguuaa 100
<210> 244
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 244
aaaacuguuu auagugaugu agaaaacccu caccuuaugg guugggauua uccuaaaugu 60
gauagagcca ugccuaacau gcuuagaauu auggccucac 100
<210> 245
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 245
cuuguucuug cucgcaaaca uacaacgugu uguagcuugu cacaccguuu cuauagauua 60
gcuaaugagu gugcucaagu auugagugaa auggucaugu 100
<210> 246
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 246
guggcgguuc acuauauguu aaaccaggug gaaccucauc aggagaugcc acaacugcuu 60
augcuaauag uguuuuuaac auuugucaag cugucacggc 100
<210> 247
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 247
cggccaaugu uaaugcacuu uuaucuacug augguaacaa aauugccgau aaguaugucc 60
gcaauuuaca acacagacuu uaugaguguc ucuauagaaa 100
<210> 248
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 248
gagauguuga cacagacuuu gugaaugagu uuuacgcaua uuugcguaaa cauuucucaa 60
ugaugauacu cucugacgau gcuguugugu guuucaauag 100
<210> 249
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 249
gcacuuaugc aucucaaggu cuaguggcua gcauaaagaa cuuuaaguca guucuuuauu 60
aucaaaacaa uguuuuuaug ucugaagcaa aauguuggac 100
<210> 250
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 250
cugagacuga ccuuacuaaa ggaccucaug aauuuugcuc ucaacauaca augcuaguua 60
aacaggguga ugauuaugug uaccuuccuu acccagaucc 100
<210> 251
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 251
ccaucaagaa uccuaggggc cggcuguuuu guagaugaua ucguaaaaac agaugguaca 60
cuuaugauug aacgguucgu gucuuuagcu auagaugcuu 100
<210> 252
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 252
uacccacuua cuaaacaucc uaaucaggag uaugcugaug ucuuucauuu guacuuacaa 60
uacauaagaa agcuacauga ugaguuaaca ggacacaugu 100
<210> 253
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 253
uagacaugua uucuguuaug cuuacuaaug auaacacuuc aagguauugg gaaccugagu 60
uuuaugaggc uauguacaca ccgcauacag ucuuacaggc 100
<210> 254
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 254
gcuguugggg cuuguguucu uugcaauuca cagacuucau uaagaugugg ugcuugcaua 60
cguagaccau ucuuauguug uaaaugcugu uacgaccaug 100
<210> 255
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 255
gucauaucaa caucacauaa auuagucuug ucuguuaauc cguauguuug caaugcucca 60
gguugugaug ucacagaugu gacucaacuu uacuuaggag 100
<210> 256
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 256
gguaugagcu auuauuguaa aucacauaaa ccacccauua guuuuccauu gugugcuaau 60
ggacaaguuu uugguuuaua uaaaaauaca uguguuggua 100
<210> 257
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 257
agcgauaaug uuacugacuu uaaugcaauu gcaacaugug acuggacaaa ugcuggugau 60
uacauuuuag cuaacaccug uacugaaaga cucaagcuuu 100
<210> 258
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 258
uugcagcaga aacgcucaaa gcuacugagg agacauuuaa acugucuuau gguauugcua 60
cuguacguga agugcugucu gacagagaau uacaucuuuc 100
<210> 259
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 259
caugggaagu ugguaaaccu agaccaccac uuaaccgaaa uuaugucuuu acugguuauc 60
guguaacuaa aaacaguaaa guacaaauag gagaguacac 100
<210> 260
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 260
accuuugaaa aaggugacua uggugaugcu guuguuuacc gagguacaac aacuuacaaa 60
uuaaauguug gugauuauuu ugugcugaca ucacauacag 100
<210> 261
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 261
guaaugccau uaagugcacc uacacuagug ccacaagagc acuauguuag aauuacuggc 60
uuauacccaa cacucaauau cucagaugag uuuucuagca 100
<210> 262
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 262
caauguugca aauuaucaaa agguugguau gcaaaaguau ucuacacucc agggaccacc 60
ugguacuggu aagagucauu uugcuauugg ccuagcucuc 100
<210> 263
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 263
cuacuacccu ucugcucgca uaguguauac agcuugcucu caugccgcug uugaugcacu 60
augugagaag gcauuaaaau auuugccuau agauaaaugu 100
<210> 264
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 264
guagaauuau accugcacgu gcucguguag aguguuuuga uaaauucaaa gugaauucaa 60
cauuagaaca guaugucuuu uguacuguaa augcauugcc 100
<210> 265
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 265
ccugagacga cagcagauau aguugucuuu gaugaaauuu caauggccac aaauuaugau 60
uugaguguug ucaaugccag auuacgugcu aagcacuaug 100
<210> 266
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 266
guguacauug gcgacccugc ucaauuaccu gcaccacgca cauugcuaac uaagggcaca 60
cuagaaccag aauauuucaa uucagugugu agacuuauga 100
<210> 267
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 267
aaacuauagg uccagacaug uuccucggaa cuugucggcg uuguccugcu gaaauuguug 60
acacugugag ugcuuugguu uaugauaaua agcuuaaagc 100
<210> 268
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 268
gcacauaaag acaaaucagc ucaaugcuuu aaaauguuuu auaagggugu uaucacgcau 60
gauguuucau cugcaauuaa caggccacaa auaggcgugg 100
<210> 269
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 269
guaagagaau uccuuacacg uaacccugcu uggagaaaag cugucuuuau uucaccuuau 60
aauucacaga augcuguagc cucaaagauu uugggacuac 100
<210> 270
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 270
ccaacucaaa cuguugauuc aucacagggc ucagaauaug acuaugucau auucacucaa 60
accacugaaa cagcucacuc uuguaaugua aacagauuua 100
<210> 271
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 271
uguugcuauu accagagcaa aaguaggcau acuuugcaua augucugaua gagaccuuua 60
ugacaaguug caauuuacaa gucuugaaau uccacguagg 100
<210> 272
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 272
aggaaugugg caacuuuaca agcugaaaau guaacaggac ucuuuaaaga uuguaguaag 60
guaaucacug gguuacaucc uacacaggca ccuacacacc 100
<210> 273
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 273
ccucaguguu gacacuaaau ucaaaacuga agguuuaugu guugacauac cuggcauacc 60
uaaggacaug accuauagaa gacucaucuc uaugaugggu 100
<210> 274
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 274
uaaaaugaau uaucaaguua augguuaccc uaacauguuu aucacccgcg aagaagcuau 60
aagacaugua cgugcaugga uuggcuucga ugucgagggg 100
<210> 275
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 275
gggugucaug cuacuagaga agcuguuggu accaauuuac cuuuacagcu agguuuuucu 60
acagguguua accuaguugc uguaccuaca gguuauguug 100
<210> 276
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 276
gauacaccua auaauacaga uuuuuccaga guuagugcua aaccaccgcc uggagaucaa 60
uuuaaacacc ucauaccacu uauguacaaa ggacuuccuu 100
<210> 277
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 277
uggaauguag ugcguauaaa gauuguacaa auguuaagug acacacuuaa aaaucucucu 60
gacagagucg uauuugucuu augggcacau ggcuuugagu 100
<210> 278
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 278
gacaucuaug aaguauuuug ugaaaauagg accugagcgc accuguuguc uaugugauag 60
acgugccaca ugcuuuucca cugcuucaga cacuuaugcc 100
<210> 279
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 279
gccuguuggc aucauucuau uggauuugau uacgucuaua auccguuuau gauugauguu 60
caacaauggg guuuuacagg uaaccuacaa agcaaccaug 100
<210> 280
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 280
gaucuguauu gucaagucca ugguaaugca cauguagcua guugugaugc aaucaugacu 60
aggugucuag cuguccacga gugcuuuguu aagcguguug 100
<210> 281
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 281
gacuggacua uugaauaucc uauaauuggu gaugaacuga agauuaaugc ggcuuguaga 60
aagguucaac acaugguugu uaaagcugca uuauuagcag 100
<210> 282
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 282
gacaaauucc caguucuuca cgacauuggu aacccuaaag cuauuaagug uguaccucaa 60
gcugauguag aauggaaguu cuaugaugca cagccuugua 100
<210> 283
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 283
agugacaaag cuuauaaaau agaagaauua uucuauucuu augccacaca uucugacaaa 60
uucacagaug guguaugccu auuuuggaau ugcaaugucg 100
<210> 284
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 284
cgauagauau ccugcuaauu ccauuguuug uagauuugac acuagagugc uaucuaaccu 60
uaacuugccu gguugugaug guggcaguuu guauguaaau 100
<210> 285
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 285
aacaugcauu ccacacacca gcuuuugaua aaagugcuuu uguuaauuua aaacaauuac 60
cauuuuucua uuacucugac aguccaugug agucucaugg 100
<210> 286
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 286
ggaaaacaag uagugucaga uauagauuau guaccacuaa agucugcuac guguauaaca 60
cguugcaauu uagguggugc ugucuguaga caucaugcua 100
<210> 287
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 287
augaguacag auuguaucuc gaugcuuaua acaugaugau cucagcuggc uuuagcuugu 60
ggguuuacaa acaauuugau acuuauaacc ucuggaacac 100
<210> 288
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 288
cacuuuuaca agacuucaga guuuagaaaa uguggcuuuu aauguuguaa auaagggaca 60
cuuugaugga caacagggug aaguaccagu uucuaucauu 100
<210> 289
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 289
uaacacuguu uacacaaaag uugauggugu ugauguagaa uuguuugaaa auaaaacaac 60
auuaccuguu aauguagcau uugagcuuug ggcuaagcgc 100
<210> 290
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 290
cgcaacauua aaccaguacc agaggugaaa auacucaaua auuugggugu ggacauugcu 60
gcuaauacug ugaucuggga cuacaaaaga gaugcuccag 100
<210> 291
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 291
gcacauauau cuacuauugg uguuuguucu augacugaca uagccaagaa accaacugaa 60
acgauuugug caccacucac ugucuuuuuu gaugguagag 100
<210> 292
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 292
guugaugguc aaguagacuu auuuagaaau gcccguaaug guguucuuau uacagaaggu 60
aguguuaaag guuuacaacc aucuguaggu cccaaacaag 100
<210> 293
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 293
gcuagucuua auggagucac auuaauugga gaagccguaa aaacacaguu caauuauuau 60
aagaaaguug augguguugu ccaacaauua ccugaaacuu 100
<210> 294
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 294
uacuuuacuc agaguagaaa uuuacaagaa uuuaaaccca ggagucaaau ggaaauugau 60
uucuuagaau uagcuaugga ugaauucauu gaacgguaua 100
<210> 295
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 295
aaauuagaag gcuaugccuu cgaacauauc guuuauggag auuuuaguca uagucaguua 60
ggugguuuac aucuacugau uggacuagcu aaacguuuua 100
<210> 296
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 296
aggaaucacc uuuugaauua gaagauuuua uuccuaugga caguacaguu aaaaacuauu 60
ucauaacaga ugcgcaaaca gguucaucua agugugugug 100
<210> 297
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 297
guucuguuau ugauuuauua cuugaugauu uuguugaaau aauaaaaucc caagauuuau 60
cuguaguuuc uaagguuguc aaagugacua uugacuauac 100
<210> 298
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 298
cagaaauuuc auuuaugcuu ugguguaaag auggccaugu agaaacauuu uacccaaaau 60
uacaaucuag ucaagcgugg caaccgggug uugcuaugcc 100
<210> 299
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 299
ccuaaucuuu acaaaaugca aagaaugcua uuagaaaagu gugaccuuca aaauuauggu 60
gauagugcaa cauuaccuaa aggcauaaug augaaugucg 100
<210> 300
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 300
gcaaaauaua cucaacugug ucaauauuua aacacauuaa cauuagcugu acccuauaau 60
augagaguua uacauuuugg ugcugguucu gauaaaggag 100
<210> 301
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 301
guugcaccag guacagcugu uuuaagacag ugguugccua cggguacgcu gcuugucgau 60
ucagaucuua augacuuugu cucugaugca gauucaacuu 100
<210> 302
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 302
uugauuggug auugugcaac uguacauaca gcuaauaaau gggaucucau uauuagugau 60
auguacgacc cuaagacuaa aaauguuaca aaagaaaaug 100
<210> 303
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 303
auugaugcaa aagagggcgg uaauuuuaac uaucuuuacc gucucuuuag aaaagcuaau 60
cuuaaacccu uugagaggga uaucucaacu gaaauuuacc 100
<210> 304
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 304
caagcaggca gcaaaccuug uaauggucaa acuggucuaa auugcuacua cccacuuuau 60
agauauggau uuuacccuac ugaugguguu ggucaccaac 100
<210> 305
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 305
caauuucaac uuuaaugguu uaacuggcac agguguccuc acagagucua auaaaaaguu 60
ucuaccuuuc caacaauuug guagagacau ugcagacacu 100
<210> 306
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 306
ugauccuuug caaccagaac uugauucauu caaggaggag uuggauaaau acuuuaaaaa 60
ucauacauca ccugauguag auuuagguga cauuucuggc 100
<210> 307
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 307
acuucacagu ggcuaacaaa uaucuuuggc acugucuaug agcaacucaa accuguucuu 60
gauuggcucg aagagaaauu caaggaaggu guagaguuuc 100
<210> 308
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 308
acauuuguca cacacucaaa gggauuguau agaaagugug uuaaacccaa agaagaaacu 60
ggcuuacuca ugccucugaa agcuccaaaa gaaauuauuu 100
<210> 309
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 309
gauagucaac aaacugucgu ucaagaagau gacagugaag uuaaucagac aacuaucacu 60
caaucaauug cugagguuca accucaguua gagauggaac 100
<210> 310
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 310
gccacugcau uauuaacacu acagcaaaua gaguugaaau uuaacccacc ugcuuuacaa 60
gaugccuacu acagggcaag agcuggugaa gcugcuaauu 100
<210> 311
<211> 100
<212> RNA
<213> 人工序列(Artificial Sequence)
<400> 311
ccaucaggua aaguugaggg uugcauggua caaguuacuu gugguacaac cacacuuaau 60
ggucuuuggc uugaugaugu agucuacugu ccaagacaug 100
<210> 312
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 312
aatgatacgg cgaccaccga gatct 25
<210> 313
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 313
caagcagaag acggcatacg agat 24
<210> 314
<211> 57
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 314
aatgatacgg cgaccaccga gatctacacc ggttgttaca ctctttccct acacgac 57
<210> 315
<211> 57
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 315
aatgatacgg cgaccaccga gatctacacg tgcttacaca ctctttccct acacgac 57
<210> 316
<211> 57
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 316
aatgatacgg cgaccaccga gatctacact cgtggataca ctctttccct acacgac 57
<210> 317
<211> 57
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 317
aatgatacgg cgaccaccga gatctacacg tgaagtgaca ctctttccct acacgac 57
<210> 318
<211> 57
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 318
aatgatacgg cgaccaccga gatctacact tggtctcaca ctctttccct acacgac 57
<210> 319
<211> 57
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 319
aatgatacgg cgaccaccga gatctacacg tgccataaca ctctttccct acacgac 57
<210> 320
<211> 57
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 320
aatgatacgg cgaccaccga gatctacacc tacagtgaca ctctttccct acacgac 57
<210> 321
<211> 57
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 321
aatgatacgg cgaccaccga gatctacacc cagtgttaca ctctttccct acacgac 57
<210> 322
<211> 57
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 322
aatgatacgg cgaccaccga gatctacact taccgagaca ctctttccct acacgac 57
<210> 323
<211> 57
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 323
aatgatacgg cgaccaccga gatctacacg attaccgaca ctctttccct acacgac 57
<210> 324
<211> 53
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 324
caagcagaag acggcatacg agatatcgat cggtgactgg agttcagacg tgt 53
<210> 325
<211> 53
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 325
caagcagaag acggcatacg agatcggtca tagtgactgg agttcagacg tgt 53
<210> 326
<211> 53
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 326
caagcagaag acggcatacg agatagtcgc ttgtgactgg agttcagacg tgt 53
<210> 327
<211> 53
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 327
caagcagaag acggcatacg agatacagca acgtgactgg agttcagacg tgt 53
<210> 328
<211> 53
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 328
caagcagaag acggcatacg agatgtgctt acgtgactgg agttcagacg tgt 53
<210> 329
<211> 53
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 329
caagcagaag acggcatacg agatcctcag ttgtgactgg agttcagacg tgt 53
<210> 330
<211> 53
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 330
caagcagaag acggcatacg agatattcga gggtgactgg agttcagacg tgt 53
<210> 331
<211> 53
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 331
caagcagaag acggcatacg agataactgg tggtgactgg agttcagacg tgt 53
<210> 332
<211> 53
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 332
caagcagaag acggcatacg agataacctc ctgtgactgg agttcagacg tgt 53
<210> 333
<211> 53
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 333
caagcagaag acggcatacg agataagagc cagtgactgg agttcagacg tgt 53
<210> 334
<211> 37
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 334
acactctttc cctacacgac gctcttccga tctnnnt 37
<220>
<221> misc_feature
<222> (34,35,36)
<223> n=a或c或t或g。
<210> 335
<211> 37
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 335
nnnagatcgg aagagcacac gtctgaactc cagtcac 37
<220>
<221> misc_feature
<222> (1,2,3)
<223> n=a或c或t或g。
Claims (10)
1.一种新型β冠状病毒变异检测序列组合，所述序列组合包括RNA探针组，其特征在于，所述的RNA探针组包括311条RNA探针或对应的311条DNA探针，所述的RNA探针序列如SEQ IDNO.1～SEQ ID NO.311所示。
2.根据权利要求1所述的新型β冠状病毒变异检测序列组合，其特征在于，所述序列组合还包括接头序列、第一引物组和第二引物组；所述接头序列包括SEQ ID NO.334和SEQ IDNO.335；所述第一引物组选自序列为SEQ ID NO.314～SEQ ID NO.333的引物对，用于文库扩增。其中，SEQ ID NO.314和324、SEQ ID NO.315和325、SEQ ID NO.316和326、SEQ IDNO.317和327、SEQ ID NO.318和328、SEQ ID NO.319和329、SEQ ID NO.320和330、SEQ IDNO.321和331、SEQ ID NO.322和332、SEQ ID NO.323和333配对使用；所述第二引物组包括正向引物和反向引物，用于杂交捕获文库序列的扩增。优选地，所述正向引物序列为SEQ IDNO.312，所述反向引物序列为SEQ ID NO.313。
3.根据权利要求1或2所述的新型β冠状病毒变异检测序列组合，其特征在于，所述的RNA探针为生物素标记的RNA探针。
4.一种新型β冠状病毒变异检测试剂盒，其特征在于，所述试剂盒包括根据权利要求1-3任一项所述的新型β冠状病毒变异检测序列组合。
5.根据权利要求4所述的新型β冠状病毒变异检测试剂盒，其特征在于，所述的试剂盒还包括：封闭缓冲液、RNA酶封闭液、杂交缓冲液、结合缓冲液、1倍浓度的SSC漂洗液、0.1倍浓度的SSC漂洗液、PCR反应液和TE缓冲液。
6.根据权利要求5所述的新型β冠状病毒变异检测试剂盒，其特征在于，所述的封闭缓冲液包括人cot-1DNA、鲑鱼精DNA和通用型封闭序列。
7.一种新型β冠状病毒变异检测方法，其特征在于，所述方法包括：
(1)提取病毒样本的RNA；
(2)构建总RNA文库，总RNA文库包括由RNA逆转录而成的cDNA双链序列和接头；
(3)对所述RNA链特异性或非链特异性文库用RNA探针组进行两轮捕获和捕获后扩增，所述的RNA探针组包括311条RNA探针或对应的311条DNA探针，所述的RNA探针序列如SEQ IDNO.1～SEQ ID NO.311所示；
(4)对所述两轮捕获后扩增的扩增物进行测序。
8.根据权利要求7的方法，其特征在于，所述方法还包括：对所述测序结果进行分析。
9.根据权利要求8的方法，其特征在于，所述分析包括：
(1)将测序数据定位到SARS-CoV-2的基因组数据MN908947.3的相应位置上，同时进行各项指标统计，包括比对率、覆盖率、重复率、捕获率、平均深度、均一性指标；
(2)将测序数据比对人类参考基因组，同时分离出比对不上人类参考基因组的结果；
(3)将上一步分离出的结果比对病毒库，选取比对到病毒库的结果，利用Trinity软件进行组装，得到病毒的组装结果；
(4)将组装结果比对病毒库，确定病毒的分型结果是否为MN908947.3；
(5)选取(4)比对到MN908947.3基因组上的结果，进行突变寻找，突变包括SNP和InDel两种类型，确定突变位点发生的基因、位置、功能、氨基酸改变等。
10.RNA探针组用于制备检测新型β冠状病毒变异的制剂的用途，其特征在于，所述的RNA探针组包括311条RNA探针或对应的311条DNA探针，所述的RNA探针序列如SEQ ID NO.1～SEQ ID NO.311所示；所述检测新型β冠状病毒变异通过根据权利要求7-9任一项的方法进行。
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