CN111214512A - 一种马齿苋提取物的制备方法 - Google Patents
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- 238000002360 preparation method Methods 0.000 title claims abstract description 10
- 239000000284 extract Substances 0.000 claims abstract description 15
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000002244 precipitate Substances 0.000 claims abstract description 12
- 238000000194 supercritical-fluid extraction Methods 0.000 claims abstract description 12
- 239000011347 resin Substances 0.000 claims abstract description 8
- 229920005989 resin Polymers 0.000 claims abstract description 8
- 238000001035 drying Methods 0.000 claims abstract description 6
- 238000000605 extraction Methods 0.000 claims abstract description 6
- 239000007788 liquid Substances 0.000 claims abstract description 6
- 238000010298 pulverizing process Methods 0.000 claims abstract description 6
- 238000001179 sorption measurement Methods 0.000 claims abstract description 6
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 15
- 238000000034 method Methods 0.000 claims description 5
- 238000001953 recrystallisation Methods 0.000 claims description 5
- 239000002904 solvent Substances 0.000 claims description 2
- 238000011112 process operation Methods 0.000 abstract 1
- 241000219304 Portulacaceae Species 0.000 description 3
- 239000003480 eluent Substances 0.000 description 3
- 238000004128 high performance liquid chromatography Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 235000001855 Portulaca oleracea Nutrition 0.000 description 2
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- 239000000194 fatty acid Substances 0.000 description 2
- 150000004665 fatty acids Chemical class 0.000 description 2
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- 238000007254 oxidation reaction Methods 0.000 description 2
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
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- A61P39/00—General protective or antinoxious agents
- A61P39/06—Free radical scavengers or antioxidants
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/37—Extraction at elevated pressure or temperature, e.g. pressurized solvent extraction [PSE], supercritical carbon dioxide extraction or subcritical water extraction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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Abstract
本发明公开了一种马齿苋提取物的制备方法。制备方法包括以下步骤：取马齿苋粉碎40‑60目，加入超临界萃取釜中，在萃取压力5‑15MPa、温度60‑80℃的条件下，通入液态CO2超临界萃取3‑5小时，在压力15‑20MPa，温度60‑70℃解析，得萃取物；上述萃取物，加入大孔树脂中吸附，70%乙醇溶液洗脱，收集洗脱液浓缩，得沉淀物；上述沉淀物经重结晶，干燥即得马齿苋提取物。采用本发明制备方法，工艺操作简便、得率高，污染小，适合高纯度马齿苋提取物的生产。
Description
本发明公开了一种植物提取物的制备方法及应用，特别涉及一种马齿苋提取物的制备方法。
背景技术
马齿苋为马齿苋科一年生草本植物。为药食两用植物。全草供药用，有清热利湿、解毒消肿、消炎、止渴、利尿作用；马齿苋还含有丰富的SL3脂肪酸及维生素A样物质。SL3脂肪酸是形成细胞膜，尤其是脑细胞膜与眼细胞膜所必需的物质；维生素A样物质能维持上皮组织如皮肤、角膜及结合膜的正常机能，参与视紫质的合成，增强视网膜感光性能，也参与体内许多氧化过程。
马齿苋中含有的黄酮和皂甙具有去自由基和抗氧化作用，可以延缓皮肤衰老。
马齿苋提取物具有在冬季防干燥老化、增加皮肤舒适度以及清除自由基，改善皮肤舒适感等的综合性能。
发明内容
为了实现上述目的，本发明采用以下技术方案：
马齿苋提取物的制备方法，其特征在于，包括以下步骤：
a.取马齿苋粉碎40-60目，加入超临界萃取釜中，在萃取压力5-15MPa、温度60-80℃的条件下，通入液态CO2超临界萃取3-5小时，在压力15-20MPa，温度60-70℃解析，得萃取物；
b.上述萃取物，加入大孔树脂中吸附，70%乙醇溶液洗脱，收集洗脱液浓缩，得沉淀物；
c.上述沉淀物经重结晶，干燥即得马齿苋提取物。
步骤b中所述的大孔树脂为HZ816和HPD100中的一种。
步骤c中重结晶溶剂为70%甲醇。
采用本法制备马齿苋提取物，工艺简便易操作，制备量大，产品得率高，而且低污染。
下面将结合具体实施方式进一步说明本发明，但本发明要求保护的范围并不局限于下列实施方式。
具体实施方式
实施例1：
取马齿苋粉碎40目，加入超临界萃取釜中，在萃取压力5MPa、温度60℃的条件下，通入液态CO2超临界萃取3小时，在压力15MPa，温度60℃解析，得萃取物，上述萃取物，加入大孔树脂HZ816中吸附，70%乙醇溶液洗脱，收集洗脱液浓缩，得沉淀物，上述沉淀物加入70%甲醇溶液重结晶，干燥即得马齿苋提取物，HPLC测定含量。
实施例2：
取马齿苋粉碎60目，加入超临界萃取釜中，在萃取压力15MPa、温度80℃的条件下，通入液态CO2超临界萃取5小时，在压力20MPa，温度70℃解析，得萃取物，上述萃取物，加入大孔树脂HPD100中吸附，70%乙醇溶液洗脱，收集洗脱液浓缩，得沉淀物，上述沉淀物加入70%甲醇溶液重结晶，干燥即得马齿苋提取物，HPLC测定含量。
实施例3：
取马齿苋粉碎50目，加入超临界萃取釜中，在萃取压力10MPa、温度70℃的条件下，通入液态CO2超临界萃取4小时，在压力20MPa，温度60℃解析，得萃取物，上述萃取物，加入大孔树脂HZ816中吸附，70%乙醇溶液洗脱，收集洗脱液浓缩，得沉淀物，上述沉淀物加入70%甲醇溶液重结晶，干燥即得马齿苋提取物，HPLC测定含量。
Claims (3)
1.一种马齿苋提取物的制备方法，其特征在于包括以下步骤：
a.取马齿苋粉碎40-60目，加入超临界萃取釜中，在萃取压力5-15MPa、温度60-80℃的条件下，通入液态CO2超临界萃取3-5小时，在压力15-20MPa，温度60-70℃解析，得萃取物；
b.上述萃取物，加入大孔树脂中吸附，70%乙醇溶液洗脱，收集洗脱液浓缩，得沉淀物；
c.上述沉淀物经重结晶，干燥即得马齿苋提取物。
2.根据权利要求1所述的马齿苋提取物的制备方法，其特征在于：步骤b中所述的大孔树脂为HZ816和HPD100中的一种。
3.根据权利要求1所述的马齿苋提取物的制备方法，其特征在于：步骤c中重结晶溶剂为70%甲醇。
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Application publication date: 20200602 |