CN111201428B - 对载玻片上的有机材料进行染色的设备和方法 - Google Patents
对载玻片上的有机材料进行染色的设备和方法 Download PDFInfo
- Publication number
- CN111201428B CN111201428B CN201880065436.5A CN201880065436A CN111201428B CN 111201428 B CN111201428 B CN 111201428B CN 201880065436 A CN201880065436 A CN 201880065436A CN 111201428 B CN111201428 B CN 111201428B
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- 238000010186 staining Methods 0.000 title claims abstract description 66
- 238000000034 method Methods 0.000 title claims description 35
- 239000011521 glass Substances 0.000 title description 11
- 239000011368 organic material Substances 0.000 title description 5
- 239000007788 liquid Substances 0.000 claims abstract description 105
- 238000003892 spreading Methods 0.000 claims abstract description 53
- 230000007480 spreading Effects 0.000 claims abstract description 52
- 238000006073 displacement reaction Methods 0.000 claims abstract description 40
- 239000012530 fluid Substances 0.000 claims abstract description 16
- 239000012192 staining solution Substances 0.000 claims abstract description 15
- 239000012487 rinsing solution Substances 0.000 claims abstract description 4
- 238000004140 cleaning Methods 0.000 claims description 25
- 238000002156 mixing Methods 0.000 claims description 11
- 230000009471 action Effects 0.000 claims description 7
- 239000012535 impurity Substances 0.000 claims description 5
- 238000004043 dyeing Methods 0.000 description 15
- 239000000975 dye Substances 0.000 description 14
- 238000011109 contamination Methods 0.000 description 9
- 239000006185 dispersion Substances 0.000 description 8
- 238000001035 drying Methods 0.000 description 8
- 210000004027 cell Anatomy 0.000 description 7
- 239000003795 chemical substances by application Substances 0.000 description 7
- 239000003086 colorant Substances 0.000 description 7
- 239000002699 waste material Substances 0.000 description 7
- 230000008901 benefit Effects 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 238000004458 analytical method Methods 0.000 description 5
- 238000007654 immersion Methods 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 238000013519 translation Methods 0.000 description 4
- 210000004369 blood Anatomy 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 239000007921 spray Substances 0.000 description 3
- 238000007447 staining method Methods 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000009792 diffusion process Methods 0.000 description 2
- 239000003085 diluting agent Substances 0.000 description 2
- 238000011068 loading method Methods 0.000 description 2
- 238000010422 painting Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000005086 pumping Methods 0.000 description 2
- 238000010926 purge Methods 0.000 description 2
- 238000002791 soaking Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 238000002738 Giemsa staining Methods 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 239000010796 biological waste Substances 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000000265 homogenisation Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 201000008827 tuberculosis Diseases 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 238000011121 vaginal smear Methods 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
- G01N1/312—Apparatus therefor for samples mounted on planar substrates
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
- G01N2001/317—Apparatus therefor spraying liquids onto surfaces
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pathology (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR1759397 | 2017-10-06 | ||
| FR1759397A FR3072171B1 (fr) | 2017-10-06 | 2017-10-06 | Dispositif et procede de coloration d'un element organique sur une lame |
| PCT/FR2018/052480 WO2019069038A1 (fr) | 2017-10-06 | 2018-10-08 | Dispositif et procede de coloration d'un materiau organique sur une lame |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111201428A CN111201428A (zh) | 2020-05-26 |
| CN111201428B true CN111201428B (zh) | 2024-03-05 |
Family
ID=60888490
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201880065436.5A Active CN111201428B (zh) | 2017-10-06 | 2018-10-08 | 对载玻片上的有机材料进行染色的设备和方法 |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US20200278277A1 (es) |
| EP (1) | EP3692354B1 (es) |
| JP (1) | JP7337815B2 (es) |
| KR (1) | KR102647051B1 (es) |
| CN (1) | CN111201428B (es) |
| AU (1) | AU2018346569B2 (es) |
| BR (1) | BR112020006778A2 (es) |
| CA (1) | CA3078319C (es) |
| ES (1) | ES2949482T3 (es) |
| FR (1) | FR3072171B1 (es) |
| MX (1) | MX2020003646A (es) |
| RU (1) | RU2763339C2 (es) |
| WO (1) | WO2019069038A1 (es) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112113818A (zh) * | 2020-10-09 | 2020-12-22 | 嘉兴晶铸生物科技有限公司 | 一种恒温全自动化细胞制片一体化装置及制片方法 |
Citations (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1980002104A1 (en) * | 1979-04-02 | 1980-04-16 | W Ries | Process and device for cleaning and keeping the cleanliness of surfaces |
| EP0417006A2 (en) * | 1989-09-06 | 1991-03-13 | Toa Medical Electronics Co., Ltd. | Synthetic apparatus for inspection of blood |
| US5650332A (en) * | 1995-11-14 | 1997-07-22 | Coulter International Corp. | Method for the preparation of microscope slides |
| US5854075A (en) * | 1995-06-07 | 1998-12-29 | Alpha Scientific Instruments, Inc. | Automatic blood film preparation method |
| JP2001091423A (ja) * | 1999-09-28 | 2001-04-06 | Bekkuman Koorutaa Kk | 自動標本作成装置 |
| JP2005342888A (ja) * | 2005-07-05 | 2005-12-15 | Hitachi Ltd | クリーニング装置 |
| CN102192852A (zh) * | 2010-03-11 | 2011-09-21 | 希森美康株式会社 | 涂片染色装置、制备装置、处理系统及染色条件决定方法 |
| CN103149070A (zh) * | 2013-03-11 | 2013-06-12 | 黄石市第一医院 | 一种自动染片机 |
| CN103229349A (zh) * | 2010-11-25 | 2013-07-31 | 东京毅力科创株式会社 | 色素吸附装置、色素吸附方法及基板处理装置 |
| CN103299173A (zh) * | 2010-11-10 | 2013-09-11 | 体质医学股份有限公司 | 制备用于检查的生物标本的自动化系统及方法 |
| CN104053995A (zh) * | 2011-11-16 | 2014-09-17 | 莱卡生物系统墨尔本私人有限公司 | 处理载玻片上的组织样本的自动系统和方法 |
| WO2014145614A1 (en) * | 2013-03-15 | 2014-09-18 | Angros Lee H | Method of removing floatation liquid |
| CN104884932A (zh) * | 2012-11-01 | 2015-09-02 | 莱卡生物系统墨尔本私人有限公司 | 流体输送系统 |
| CN105021442A (zh) * | 2015-06-05 | 2015-11-04 | 青岛大学 | 一种全自动医学检验血液堆片染色机 |
| CN105518466A (zh) * | 2013-08-09 | 2016-04-20 | 诺维茨公司 | 用于清洗吸取-分配设备的方法与设备 |
| CN106769351A (zh) * | 2015-11-23 | 2017-05-31 | 内蒙古蒙牛乳业（集团）股份有限公司 | 微生物染色架 |
| CN106840815A (zh) * | 2017-01-20 | 2017-06-13 | 杜鹏 | 一种自动化医学检验血液堆片染色机 |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AUPO439496A0 (en) * | 1996-12-24 | 1997-01-23 | Australian Biomedical Corporation Pty. Ltd. | Smearer mechanism |
| WO2002078906A2 (en) * | 2001-03-29 | 2002-10-10 | Cellect Technologies Corp. | Methods devices and systems for sorting and separating particles |
| US7297311B2 (en) * | 2001-06-29 | 2007-11-20 | Sysmex Corporation | Automatic smear preparing apparatus and automatic sample analysis system having the same |
| RU2440562C2 (ru) * | 2009-11-26 | 2012-01-20 | Общество с ограниченной ответственностью ЭМКО Электромеханическая компания | Автомат для окраски мазков на предметных стеклах |
| US8765476B2 (en) * | 2009-12-22 | 2014-07-01 | Biocare Medical, Llc | Methods and systems for efficient automatic slide staining in immunohistochemistry sample processing |
| CN101957279A (zh) * | 2010-09-19 | 2011-01-26 | 王珑 | 全自动lct液基薄层细胞和细胞dna快速制片与染色一体机 |
| CN102759474A (zh) * | 2012-06-13 | 2012-10-31 | 长沙康柏恩医疗科技有限公司 | 运用注射移液原理的液基细胞制片染色机 |
| HU230739B1 (hu) * | 2013-02-28 | 2018-01-29 | 3Dhistech Kft. | Berendezés és eljárás tárgylemezek automatikus festésére, fedésére, digitalizálására integrált tárgylemez festő-fedő-digitalizáló automata berendezés segítségével |
| AU2015206277A1 (en) * | 2014-01-17 | 2016-08-11 | William Eugene Campbell | Methods and systems for slide processing |
| CN105890959A (zh) * | 2014-11-24 | 2016-08-24 | 王素蓉 | 染色仪及染色仪的控制方法 |
| EP3346254B1 (en) * | 2015-08-31 | 2022-03-23 | Sysmex Corporation | Smear sample preparation apparatus and smear sample preparing method |
| JP6242432B2 (ja) * | 2016-04-28 | 2017-12-06 | シスメックス株式会社 | 検体塗抹装置および検体塗抹方法 |
| CN106018045A (zh) * | 2016-07-04 | 2016-10-12 | 邱加伟 | 用于细胞制片染色的移液机构 |
| JP6925152B2 (ja) * | 2017-04-06 | 2021-08-25 | シスメックス株式会社 | 塗抹部材の洗浄方法および塗抹標本作製装置 |
| WO2020257963A1 (zh) * | 2019-06-24 | 2020-12-30 | 深圳迈瑞生物医疗电子股份有限公司 | 一种推片机及样本染色方法 |
| EP3809112A1 (en) * | 2019-10-18 | 2021-04-21 | Sysmex Corporation | Washing method for staining bath in smear sample preparing apparatus, and smear sample preparing apparatus |
-
2017
- 2017-10-06 FR FR1759397A patent/FR3072171B1/fr active Active
-
2018
- 2018-10-08 RU RU2020112521A patent/RU2763339C2/ru active
- 2018-10-08 BR BR112020006778-4A patent/BR112020006778A2/pt active Search and Examination
- 2018-10-08 US US16/753,854 patent/US20200278277A1/en active Pending
- 2018-10-08 KR KR1020207009385A patent/KR102647051B1/ko active IP Right Grant
- 2018-10-08 EP EP18804367.3A patent/EP3692354B1/fr active Active
- 2018-10-08 JP JP2020540852A patent/JP7337815B2/ja active Active
- 2018-10-08 CN CN201880065436.5A patent/CN111201428B/zh active Active
- 2018-10-08 WO PCT/FR2018/052480 patent/WO2019069038A1/fr unknown
- 2018-10-08 CA CA3078319A patent/CA3078319C/fr active Active
- 2018-10-08 AU AU2018346569A patent/AU2018346569B2/en active Active
- 2018-10-08 ES ES18804367T patent/ES2949482T3/es active Active
- 2018-10-08 MX MX2020003646A patent/MX2020003646A/es unknown
Patent Citations (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1980002104A1 (en) * | 1979-04-02 | 1980-04-16 | W Ries | Process and device for cleaning and keeping the cleanliness of surfaces |
| EP0417006A2 (en) * | 1989-09-06 | 1991-03-13 | Toa Medical Electronics Co., Ltd. | Synthetic apparatus for inspection of blood |
| US5854075A (en) * | 1995-06-07 | 1998-12-29 | Alpha Scientific Instruments, Inc. | Automatic blood film preparation method |
| US5650332A (en) * | 1995-11-14 | 1997-07-22 | Coulter International Corp. | Method for the preparation of microscope slides |
| JP2001091423A (ja) * | 1999-09-28 | 2001-04-06 | Bekkuman Koorutaa Kk | 自動標本作成装置 |
| JP2005342888A (ja) * | 2005-07-05 | 2005-12-15 | Hitachi Ltd | クリーニング装置 |
| CN102192852A (zh) * | 2010-03-11 | 2011-09-21 | 希森美康株式会社 | 涂片染色装置、制备装置、处理系统及染色条件决定方法 |
| CN103299173A (zh) * | 2010-11-10 | 2013-09-11 | 体质医学股份有限公司 | 制备用于检查的生物标本的自动化系统及方法 |
| CN103229349A (zh) * | 2010-11-25 | 2013-07-31 | 东京毅力科创株式会社 | 色素吸附装置、色素吸附方法及基板处理装置 |
| CN104053995A (zh) * | 2011-11-16 | 2014-09-17 | 莱卡生物系统墨尔本私人有限公司 | 处理载玻片上的组织样本的自动系统和方法 |
| CN104884932A (zh) * | 2012-11-01 | 2015-09-02 | 莱卡生物系统墨尔本私人有限公司 | 流体输送系统 |
| CN103149070A (zh) * | 2013-03-11 | 2013-06-12 | 黄石市第一医院 | 一种自动染片机 |
| WO2014145614A1 (en) * | 2013-03-15 | 2014-09-18 | Angros Lee H | Method of removing floatation liquid |
| CN105518466A (zh) * | 2013-08-09 | 2016-04-20 | 诺维茨公司 | 用于清洗吸取-分配设备的方法与设备 |
| CN105021442A (zh) * | 2015-06-05 | 2015-11-04 | 青岛大学 | 一种全自动医学检验血液堆片染色机 |
| CN106769351A (zh) * | 2015-11-23 | 2017-05-31 | 内蒙古蒙牛乳业（集团）股份有限公司 | 微生物染色架 |
| CN106840815A (zh) * | 2017-01-20 | 2017-06-13 | 杜鹏 | 一种自动化医学检验血液堆片染色机 |
Non-Patent Citations (1)
| Title |
|---|
| 蒲明秋,闫爱华.盖玻片染色方法的介绍.中原医刊.1995,(09),第50页. * |
Also Published As
| Publication number | Publication date |
|---|---|
| RU2020112521A3 (es) | 2021-11-08 |
| FR3072171B1 (fr) | 2021-02-19 |
| EP3692354A1 (fr) | 2020-08-12 |
| JP7337815B2 (ja) | 2023-09-04 |
| ES2949482T3 (es) | 2023-09-28 |
| WO2019069038A1 (fr) | 2019-04-11 |
| CA3078319A1 (fr) | 2019-04-11 |
| KR102647051B1 (ko) | 2024-03-13 |
| RU2763339C2 (ru) | 2021-12-28 |
| CA3078319C (fr) | 2024-03-05 |
| AU2018346569A1 (en) | 2020-04-23 |
| AU2018346569A8 (en) | 2020-05-21 |
| MX2020003646A (es) | 2020-07-29 |
| AU2018346569B2 (en) | 2023-07-20 |
| KR20200066620A (ko) | 2020-06-10 |
| RU2020112521A (ru) | 2021-11-08 |
| JP2020537159A (ja) | 2020-12-17 |
| EP3692354B1 (fr) | 2023-05-24 |
| FR3072171A1 (fr) | 2019-04-12 |
| CN111201428A (zh) | 2020-05-26 |
| AU2018346569A2 (en) | 2020-04-30 |
| US20200278277A1 (en) | 2020-09-03 |
| BR112020006778A2 (pt) | 2020-10-06 |
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