CN111133101A - 可诱导分泌抗cd47抗体的工程化免疫细胞 - Google Patents

可诱导分泌抗cd47抗体的工程化免疫细胞 Download PDF

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CN111133101A
CN111133101A CN201880062110.7A CN201880062110A CN111133101A CN 111133101 A CN111133101 A CN 111133101A CN 201880062110 A CN201880062110 A CN 201880062110A CN 111133101 A CN111133101 A CN 111133101A
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cells
car
cell
antibody
immune cell
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CN111133101B (zh
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张永亮
刘丽萍
曹卫
马玲
马安云
何佳平
沈连军
王欣欣
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Genxi Biotechnology Shanghai Co Ltd
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Abstract

提供了一种在CAR和/或外源TCR被激活时可诱导分泌抗CD47抗体的免疫细胞及其用途、含有所述免疫细胞的制剂,还提供了所述免疫细胞的制备方法以及用于所述制备方法的试剂盒。

Description

PCT国内申请,说明书已公开。

Claims (13)

  1. PCT国内申请,权利要求书已公开。
CN201880062110.7A 2017-09-27 2018-09-27 可诱导分泌抗cd47抗体的工程化免疫细胞 Active CN111133101B (zh)

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CN201710891841X 2017-09-27
PCT/CN2018/108022 WO2019062817A1 (zh) 2017-09-27 2018-09-27 可诱导分泌抗cd47抗体的工程化免疫细胞

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Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112175911B (zh) 2014-05-15 2023-10-13 新加坡国立大学 经修饰的自然杀伤细胞及其用途
SG11201908337VA (en) 2017-03-27 2019-10-30 Nat Univ Singapore Stimulatory cell lines for ex vivo expansion and activation of natural killer cells
AU2018246235B2 (en) 2017-03-27 2023-12-21 National University Of Singapore Truncated NKG2D chimeric receptors and uses thereof in natural killer cell immunotherapy
WO2019199689A1 (en) * 2018-04-09 2019-10-17 The Trustees Of The University Of Pennsylvania Methods and compositions comprising a viral vector for expression of a transgene and an effector
KR20210137085A (ko) 2019-03-05 2021-11-17 엔카르타, 인크. Cd19 유도된 키메라 항원 수용체 및 면역 요법에서 이의 용도
CN110338148A (zh) * 2019-06-21 2019-10-18 核工业总医院 基于放射远隔效应联合旁效应控制腹膜转移的方法及应用
US20220281971A1 (en) * 2019-07-17 2022-09-08 National University Of Singapore Functional Binders Synthesized and Secreted by Immune Cells
CN110256565B (zh) * 2019-08-02 2021-03-23 天津大学 抗cd47纳米抗体突变体及其应用
WO2021146487A2 (en) * 2020-01-14 2021-07-22 Synthekine, Inc. Il2 orthologs and methods of use
CN113717942B (zh) * 2020-05-26 2024-04-30 华东师范大学 一种联合嵌合抗原受体和i型干扰素的免疫治疗方法及其应用
CN116322755A (zh) * 2020-10-12 2023-06-23 南京驯鹿生物技术股份有限公司 一种结合cd70的抗体、嵌合抗原受体(car)及其应用
CN113481169B (zh) * 2021-08-11 2023-06-16 南方医科大学南方医院 一种细胞激活依赖性分泌系统及应用
CN115785268A (zh) * 2021-09-13 2023-03-14 三优生物医药(上海)有限公司 抗cd47抗体及其用途
CN113755448B (zh) * 2021-11-10 2022-02-18 广州百吉生物制药有限公司 联合表达CCR2b和CD40L的工程化免疫细胞及其制备和应用
CN114225047B (zh) * 2021-12-13 2023-12-26 安徽医科大学 一种免疫逃逸纳米制剂、制备方法及应用
CN114573710A (zh) * 2022-02-16 2022-06-03 南方医科大学珠江医院 一种靶向抗原同时外泌cd47抗体的免疫细胞及其应用
CN115154656B (zh) * 2022-06-16 2023-11-17 广州中医药大学(广州中医药研究院) 一种黑磷/生物活性玻璃抗肿瘤骨修复双功能复合支架及其制备方法及应用
WO2024074730A2 (en) * 2022-10-07 2024-04-11 Caedo Oncology As Divalent cd47 binding proteins
CN116785415B (zh) * 2023-05-04 2024-03-22 北京百普赛斯生物科技股份有限公司 一种特异性针对免疫细胞激活/增殖刺激的产品、制备及应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140301993A1 (en) * 2011-10-28 2014-10-09 The Trustees Of The University Of Pennsylvania Fully human, anti-mesothelin specific chimeric immune receptor for redirected mesothelin-expressing cell targeting
CN105874061A (zh) * 2013-02-26 2016-08-17 纪念斯隆-凯特琳癌症中心 用于免疫疗法的组合物和方法
CN105950561A (zh) * 2016-05-26 2016-09-21 江苏杰晟生物科技有限公司 一种靶向乳腺癌干细胞的双特异性嵌合抗原受体基因修饰的t淋巴细胞的制备方法及其产品

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5703055A (en) 1989-03-21 1997-12-30 Wisconsin Alumni Research Foundation Generation of antibodies through lipid mediated DNA delivery
US5399346A (en) 1989-06-14 1995-03-21 The United States Of America As Represented By The Department Of Health And Human Services Gene therapy
US5585362A (en) 1989-08-22 1996-12-17 The Regents Of The University Of Michigan Adenovirus vectors for gene therapy
US5350674A (en) 1992-09-04 1994-09-27 Becton, Dickinson And Company Intrinsic factor - horse peroxidase conjugates and a method for increasing the stability thereof
WO2001029058A1 (en) 1999-10-15 2001-04-26 University Of Massachusetts Rna interference pathway genes as tools for targeted genetic interference
US6326193B1 (en) 1999-11-05 2001-12-04 Cambria Biosciences, Llc Insect control agent
AU2001275474A1 (en) 2000-06-12 2001-12-24 Akkadix Corporation Materials and methods for the control of nematodes
JP6919118B2 (ja) * 2014-08-14 2021-08-18 ノバルティス アーゲー GFRα−4キメラ抗原受容体を用いる癌の治療
US11161907B2 (en) * 2015-02-02 2021-11-02 Novartis Ag Car-expressing cells against multiple tumor antigens and uses thereof
CN104877032B (zh) * 2015-06-26 2018-12-11 中国医学科学院血液病医院(血液学研究所) Cd33特异性嵌合抗原受体及其应用
CN106755023A (zh) * 2015-10-15 2017-05-31 中国人民解放军军事医学科学院附属医院 带安全开关的嵌合抗原受体免疫细胞及其制备方法与应用
CN105330750B (zh) * 2015-11-20 2019-02-01 上海细胞治疗研究院 一种快速中止car-t细胞杀伤作用的分子刹车及其用途
CN106047934B (zh) * 2016-08-09 2020-04-07 李因传 一种多编码框、非整合型慢病毒载体的构建和应用
CN109797171A (zh) * 2017-05-08 2019-05-24 北京东方略细胞技术有限公司 经修饰的t细胞、其制备方法及用途
CN109971721B (zh) * 2017-12-28 2023-10-31 上海细胞治疗研究院 自表达cd47抗体的间皮素特异性car-t细胞及其用途

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140301993A1 (en) * 2011-10-28 2014-10-09 The Trustees Of The University Of Pennsylvania Fully human, anti-mesothelin specific chimeric immune receptor for redirected mesothelin-expressing cell targeting
CN105874061A (zh) * 2013-02-26 2016-08-17 纪念斯隆-凯特琳癌症中心 用于免疫疗法的组合物和方法
CN105950561A (zh) * 2016-05-26 2016-09-21 江苏杰晟生物科技有限公司 一种靶向乳腺癌干细胞的双特异性嵌合抗原受体基因修饰的t淋巴细胞的制备方法及其产品

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ERIK HOOIJBERG等: "NFAT-controlled expression of GFP permits visualization and isolation of antigen-stimulated primary human T cells", 《BLOOD》 *
KOCHENDERFER, J.N.等: "Genbank:ADM64594.1", 《GENBANK》 *
RYOSUKE UCHIBORI等: "Activation signals from CD19-CAR permit NFAT-controlled inducible expression of transgenes in PBMCs", 《MOLECULAR THERAPY》 *
白银鹏等: "抗CD47靶向治疗研究现状及应用前景", 《中国肿瘤临床》 *

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