CN111133101A - 可诱导分泌抗cd47抗体的工程化免疫细胞 - Google Patents
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Abstract
提供了一种在CAR和/或外源TCR被激活时可诱导分泌抗CD47抗体的免疫细胞及其用途、含有所述免疫细胞的制剂,还提供了所述免疫细胞的制备方法以及用于所述制备方法的试剂盒。
Description
PCT国内申请,说明书已公开。
Claims (13)
- PCT国内申请,权利要求书已公开。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
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CN201710891841.XA CN109554348A (zh) | 2017-09-27 | 2017-09-27 | 可诱导分泌抗cd47抗体的工程化免疫细胞 |
CN201710891841X | 2017-09-27 | ||
PCT/CN2018/108022 WO2019062817A1 (zh) | 2017-09-27 | 2018-09-27 | 可诱导分泌抗cd47抗体的工程化免疫细胞 |
Publications (2)
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CN111133101A true CN111133101A (zh) | 2020-05-08 |
CN111133101B CN111133101B (zh) | 2022-10-28 |
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CN201880062110.7A Active CN111133101B (zh) | 2017-09-27 | 2018-09-27 | 可诱导分泌抗cd47抗体的工程化免疫细胞 |
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US (1) | US20200255803A1 (zh) |
EP (1) | EP3690033A4 (zh) |
CN (2) | CN109554348A (zh) |
WO (1) | WO2019062817A1 (zh) |
Families Citing this family (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112175911B (zh) | 2014-05-15 | 2023-10-13 | 新加坡国立大学 | 经修饰的自然杀伤细胞及其用途 |
SG11201908337VA (en) | 2017-03-27 | 2019-10-30 | Nat Univ Singapore | Stimulatory cell lines for ex vivo expansion and activation of natural killer cells |
AU2018246235B2 (en) | 2017-03-27 | 2023-12-21 | National University Of Singapore | Truncated NKG2D chimeric receptors and uses thereof in natural killer cell immunotherapy |
WO2019199689A1 (en) * | 2018-04-09 | 2019-10-17 | The Trustees Of The University Of Pennsylvania | Methods and compositions comprising a viral vector for expression of a transgene and an effector |
KR20210137085A (ko) | 2019-03-05 | 2021-11-17 | 엔카르타, 인크. | Cd19 유도된 키메라 항원 수용체 및 면역 요법에서 이의 용도 |
CN110338148A (zh) * | 2019-06-21 | 2019-10-18 | 核工业总医院 | 基于放射远隔效应联合旁效应控制腹膜转移的方法及应用 |
US20220281971A1 (en) * | 2019-07-17 | 2022-09-08 | National University Of Singapore | Functional Binders Synthesized and Secreted by Immune Cells |
CN110256565B (zh) * | 2019-08-02 | 2021-03-23 | 天津大学 | 抗cd47纳米抗体突变体及其应用 |
WO2021146487A2 (en) * | 2020-01-14 | 2021-07-22 | Synthekine, Inc. | Il2 orthologs and methods of use |
CN113717942B (zh) * | 2020-05-26 | 2024-04-30 | 华东师范大学 | 一种联合嵌合抗原受体和i型干扰素的免疫治疗方法及其应用 |
CN116322755A (zh) * | 2020-10-12 | 2023-06-23 | 南京驯鹿生物技术股份有限公司 | 一种结合cd70的抗体、嵌合抗原受体(car)及其应用 |
CN113481169B (zh) * | 2021-08-11 | 2023-06-16 | 南方医科大学南方医院 | 一种细胞激活依赖性分泌系统及应用 |
CN115785268A (zh) * | 2021-09-13 | 2023-03-14 | 三优生物医药(上海)有限公司 | 抗cd47抗体及其用途 |
CN113755448B (zh) * | 2021-11-10 | 2022-02-18 | 广州百吉生物制药有限公司 | 联合表达CCR2b和CD40L的工程化免疫细胞及其制备和应用 |
CN114225047B (zh) * | 2021-12-13 | 2023-12-26 | 安徽医科大学 | 一种免疫逃逸纳米制剂、制备方法及应用 |
CN114573710A (zh) * | 2022-02-16 | 2022-06-03 | 南方医科大学珠江医院 | 一种靶向抗原同时外泌cd47抗体的免疫细胞及其应用 |
CN115154656B (zh) * | 2022-06-16 | 2023-11-17 | 广州中医药大学(广州中医药研究院) | 一种黑磷/生物活性玻璃抗肿瘤骨修复双功能复合支架及其制备方法及应用 |
WO2024074730A2 (en) * | 2022-10-07 | 2024-04-11 | Caedo Oncology As | Divalent cd47 binding proteins |
CN116785415B (zh) * | 2023-05-04 | 2024-03-22 | 北京百普赛斯生物科技股份有限公司 | 一种特异性针对免疫细胞激活/增殖刺激的产品、制备及应用 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20140301993A1 (en) * | 2011-10-28 | 2014-10-09 | The Trustees Of The University Of Pennsylvania | Fully human, anti-mesothelin specific chimeric immune receptor for redirected mesothelin-expressing cell targeting |
CN105874061A (zh) * | 2013-02-26 | 2016-08-17 | 纪念斯隆-凯特琳癌症中心 | 用于免疫疗法的组合物和方法 |
CN105950561A (zh) * | 2016-05-26 | 2016-09-21 | 江苏杰晟生物科技有限公司 | 一种靶向乳腺癌干细胞的双特异性嵌合抗原受体基因修饰的t淋巴细胞的制备方法及其产品 |
Family Cites Families (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5703055A (en) | 1989-03-21 | 1997-12-30 | Wisconsin Alumni Research Foundation | Generation of antibodies through lipid mediated DNA delivery |
US5399346A (en) | 1989-06-14 | 1995-03-21 | The United States Of America As Represented By The Department Of Health And Human Services | Gene therapy |
US5585362A (en) | 1989-08-22 | 1996-12-17 | The Regents Of The University Of Michigan | Adenovirus vectors for gene therapy |
US5350674A (en) | 1992-09-04 | 1994-09-27 | Becton, Dickinson And Company | Intrinsic factor - horse peroxidase conjugates and a method for increasing the stability thereof |
WO2001029058A1 (en) | 1999-10-15 | 2001-04-26 | University Of Massachusetts | Rna interference pathway genes as tools for targeted genetic interference |
US6326193B1 (en) | 1999-11-05 | 2001-12-04 | Cambria Biosciences, Llc | Insect control agent |
AU2001275474A1 (en) | 2000-06-12 | 2001-12-24 | Akkadix Corporation | Materials and methods for the control of nematodes |
JP6919118B2 (ja) * | 2014-08-14 | 2021-08-18 | ノバルティス アーゲー | GFRα−4キメラ抗原受容体を用いる癌の治療 |
US11161907B2 (en) * | 2015-02-02 | 2021-11-02 | Novartis Ag | Car-expressing cells against multiple tumor antigens and uses thereof |
CN104877032B (zh) * | 2015-06-26 | 2018-12-11 | 中国医学科学院血液病医院(血液学研究所) | Cd33特异性嵌合抗原受体及其应用 |
CN106755023A (zh) * | 2015-10-15 | 2017-05-31 | 中国人民解放军军事医学科学院附属医院 | 带安全开关的嵌合抗原受体免疫细胞及其制备方法与应用 |
CN105330750B (zh) * | 2015-11-20 | 2019-02-01 | 上海细胞治疗研究院 | 一种快速中止car-t细胞杀伤作用的分子刹车及其用途 |
CN106047934B (zh) * | 2016-08-09 | 2020-04-07 | 李因传 | 一种多编码框、非整合型慢病毒载体的构建和应用 |
CN109797171A (zh) * | 2017-05-08 | 2019-05-24 | 北京东方略细胞技术有限公司 | 经修饰的t细胞、其制备方法及用途 |
CN109971721B (zh) * | 2017-12-28 | 2023-10-31 | 上海细胞治疗研究院 | 自表达cd47抗体的间皮素特异性car-t细胞及其用途 |
-
2017
- 2017-09-27 CN CN201710891841.XA patent/CN109554348A/zh active Pending
-
2018
- 2018-09-27 CN CN201880062110.7A patent/CN111133101B/zh active Active
- 2018-09-27 EP EP18860318.7A patent/EP3690033A4/en not_active Withdrawn
- 2018-09-27 WO PCT/CN2018/108022 patent/WO2019062817A1/zh unknown
- 2018-09-27 US US16/651,867 patent/US20200255803A1/en not_active Abandoned
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20140301993A1 (en) * | 2011-10-28 | 2014-10-09 | The Trustees Of The University Of Pennsylvania | Fully human, anti-mesothelin specific chimeric immune receptor for redirected mesothelin-expressing cell targeting |
CN105874061A (zh) * | 2013-02-26 | 2016-08-17 | 纪念斯隆-凯特琳癌症中心 | 用于免疫疗法的组合物和方法 |
CN105950561A (zh) * | 2016-05-26 | 2016-09-21 | 江苏杰晟生物科技有限公司 | 一种靶向乳腺癌干细胞的双特异性嵌合抗原受体基因修饰的t淋巴细胞的制备方法及其产品 |
Non-Patent Citations (4)
Title |
---|
ERIK HOOIJBERG等: "NFAT-controlled expression of GFP permits visualization and isolation of antigen-stimulated primary human T cells", 《BLOOD》 * |
KOCHENDERFER, J.N.等: "Genbank:ADM64594.1", 《GENBANK》 * |
RYOSUKE UCHIBORI等: "Activation signals from CD19-CAR permit NFAT-controlled inducible expression of transgenes in PBMCs", 《MOLECULAR THERAPY》 * |
白银鹏等: "抗CD47靶向治疗研究现状及应用前景", 《中国肿瘤临床》 * |
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