CN111051502A - 通用型嵌合抗原受体t细胞制备技术 - Google Patents

通用型嵌合抗原受体t细胞制备技术 Download PDF

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CN111051502A
CN111051502A CN201880057754.7A CN201880057754A CN111051502A CN 111051502 A CN111051502 A CN 111051502A CN 201880057754 A CN201880057754 A CN 201880057754A CN 111051502 A CN111051502 A CN 111051502A
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cell
car
hla
cells
tcr
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CN111051502B (zh
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王欣欣
施中东
刘丽萍
杨春辉
孙静
蔡松柏
曹卫
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Hunan Yuantai Biotechnology Co Ltd
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Abstract

提供一种通用型嵌合抗原受体CAR‑T细胞及其制备方法和应用。所述细胞HLA‑A/HLA‑B与TCR的结合被抑制，TCR基因表达被沉默，可以用于异体肿瘤治疗，且异体输注时不会引起GVHD及HVG反应，改善了异体CAR‑T细胞在受体体内的存活及抗肿瘤效果。

Description

PCT国内申请，说明书已公开。

Claims (10)

  1. PCT国内申请，权利要求书已公开。
CN201880057754.7A 2017-09-06 2018-09-06 通用型嵌合抗原受体t细胞制备技术 Active CN111051502B (zh)

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CN201710797952.4A CN109456943A (zh) 2017-09-06 2017-09-06 通用型嵌合抗原受体t细胞制备技术
PCT/CN2018/104418 WO2019047899A1 (zh) 2017-09-06 2018-09-06 通用型嵌合抗原受体t细胞制备技术

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109456943A (zh) * 2017-09-06 2019-03-12 亘喜生物科技（上海）有限公司 通用型嵌合抗原受体t细胞制备技术
CN109735558A (zh) * 2018-12-12 2019-05-10 中南大学 一种重组car19-il24基因、慢病毒载体、car19-il24-t细胞及应用
CN115768445A (zh) * 2021-07-01 2023-03-07 宁波茂行生物医药科技有限公司 靶向IL13Rα2的通用型CAR-T细胞及其制备方法和应用

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111826352A (zh) * 2019-04-22 2020-10-27 苏州方德门达新药开发有限公司 通用型car-t细胞、其制备及应用
WO2020227446A1 (en) * 2019-05-07 2020-11-12 The Board Of Trustees Of The Leland Stanford Junior University Enhancement of polypeptides and chimeric antigen receptors via hinge domains
CN112481310B (zh) * 2019-09-12 2023-06-02 华东师范大学 靶向hla-i类分子的通用型car-t细胞及其应用
JPWO2021106832A1 (zh) * 2019-11-25 2021-06-03
CA3163304A1 (en) * 2020-01-02 2021-07-08 Xiaoyun SHANG Modified immune effector cell and preparation method therefor
CN111393533B (zh) * 2020-04-16 2021-05-11 成都仕康美生物科技有限公司 靶向HLA-A的嵌合抗原受体、编码基因、CAR-Tregs细胞及其制备方法、用途
CA3199777A1 (en) * 2020-10-30 2022-05-05 Arbor Biotechnologies, Inc. Compositions comprising an rna guide targeting b2m and uses thereof
CN114525259A (zh) * 2020-11-03 2022-05-24 南京北恒生物科技有限公司 靶向cd7的嵌合抗原受体及其用途
US20240139321A1 (en) * 2021-07-01 2024-05-02 Ningbo T-Maximum Biopharmaceuticals Co., Ltd. Universal car-t cell targeting gd2, preparation method therefor, and application thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013074916A1 (en) * 2011-11-18 2013-05-23 Board Of Regents, The University Of Texas System Car+ t cells genetically modified to eliminate expression of t- cell receptor and/or hla
WO2016044605A1 (en) * 2014-09-17 2016-03-24 Beatty, Gregory Targeting cytotoxic cells with chimeric receptors for adoptive immunotherapy
WO2016069282A1 (en) * 2014-10-31 2016-05-06 The Trustees Of The University Of Pennsylvania Altering gene expression in modified t cells and uses thereof
WO2017093969A1 (en) * 2015-12-04 2017-06-08 Novartis Ag Compositions and methods for immunooncology

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5703055A (en) 1989-03-21 1997-12-30 Wisconsin Alumni Research Foundation Generation of antibodies through lipid mediated DNA delivery
US5399346A (en) 1989-06-14 1995-03-21 The United States Of America As Represented By The Department Of Health And Human Services Gene therapy
US5585362A (en) 1989-08-22 1996-12-17 The Regents Of The University Of Michigan Adenovirus vectors for gene therapy
US5350674A (en) 1992-09-04 1994-09-27 Becton, Dickinson And Company Intrinsic factor - horse peroxidase conjugates and a method for increasing the stability thereof
EP1235842A4 (en) 1999-10-15 2003-04-23 Univ Massachusetts GENESIS OF THE RNA INTERFERENCE PATH AS AID OF TARGETED GENTIAN INTERFERENCE
US6326193B1 (en) 1999-11-05 2001-12-04 Cambria Biosciences, Llc Insect control agent
AU2001275474A1 (en) 2000-06-12 2001-12-24 Akkadix Corporation Materials and methods for the control of nematodes
CN109456943A (zh) * 2017-09-06 2019-03-12 亘喜生物科技（上海）有限公司 通用型嵌合抗原受体t细胞制备技术

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013074916A1 (en) * 2011-11-18 2013-05-23 Board Of Regents, The University Of Texas System Car+ t cells genetically modified to eliminate expression of t- cell receptor and/or hla
WO2016044605A1 (en) * 2014-09-17 2016-03-24 Beatty, Gregory Targeting cytotoxic cells with chimeric receptors for adoptive immunotherapy
WO2016069282A1 (en) * 2014-10-31 2016-05-06 The Trustees Of The University Of Pennsylvania Altering gene expression in modified t cells and uses thereof
WO2017093969A1 (en) * 2015-12-04 2017-06-08 Novartis Ag Compositions and methods for immunooncology

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
TORIKAI 等: ""Genetic editing of HLA expression in hematopoietic stem cells to broaden their human application"", 《SCIENTIFIC REPORTS》 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109456943A (zh) * 2017-09-06 2019-03-12 亘喜生物科技（上海）有限公司 通用型嵌合抗原受体t细胞制备技术
CN109735558A (zh) * 2018-12-12 2019-05-10 中南大学 一种重组car19-il24基因、慢病毒载体、car19-il24-t细胞及应用
CN109735558B (zh) * 2018-12-12 2022-04-15 中南大学 一种重组car19-il24基因、慢病毒载体、car19-il24-t细胞及应用
CN115768445A (zh) * 2021-07-01 2023-03-07 宁波茂行生物医药科技有限公司 靶向IL13Rα2的通用型CAR-T细胞及其制备方法和应用

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US20230265154A1 (en) 2023-08-24
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EP3680328A4 (en) 2020-11-25
WO2019047899A1 (zh) 2019-03-14
CN109456943A (zh) 2019-03-12

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