CN111118100A - 用于生成植物的荧光激活细胞分选(facs)富集 - Google Patents

用于生成植物的荧光激活细胞分选(facs)富集 Download PDF

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Publication number
CN111118100A
CN111118100A CN201910796679.2A CN201910796679A CN111118100A CN 111118100 A CN111118100 A CN 111118100A CN 201910796679 A CN201910796679 A CN 201910796679A CN 111118100 A CN111118100 A CN 111118100A
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plant
gene
dna
sequence
etip
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Chinese (zh)
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G·斯潘根伯格
S·萨哈布
J·梅森
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Corteva Agriscience LLC
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Dow AgroSciences LLC
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
    • C12N15/8212—Colour markers, e.g. beta-glucoronidase [GUS], green fluorescent protein [GFP], carotenoid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Botany (AREA)
  • Physiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
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  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
CN201910796679.2A 2012-09-07 2013-09-09 用于生成植物的荧光激活细胞分选(facs)富集 Pending CN111118100A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261697890P 2012-09-07 2012-09-07
US61/697,890 2012-09-07
US14/020,694 2013-09-06
US14/020,694 US20140075593A1 (en) 2012-09-07 2013-09-06 Fluorescence activated cell sorting (facs) enrichment to generate plants
CN201380054087.4A CN104736712A (zh) 2012-09-07 2013-09-09 用于生成植物的荧光激活细胞分选(facs)富集

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CN111118100A true CN111118100A (zh) 2020-05-08

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CN201910796679.2A Pending CN111118100A (zh) 2012-09-07 2013-09-09 用于生成植物的荧光激活细胞分选(facs)富集
CN201380054087.4A Pending CN104736712A (zh) 2012-09-07 2013-09-09 用于生成植物的荧光激活细胞分选(facs)富集

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Cited By (1)

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KR102219621B1 (ko) 2021-02-24
MX367170B (es) 2019-08-07
JP6362600B2 (ja) 2018-07-25
BR112015004864A2 (pt) 2017-11-21
US20140075593A1 (en) 2014-03-13
BR112015004864B8 (pt) 2023-05-16
RU2015112573A (ru) 2016-10-27
TWI670004B (zh) 2019-09-01
AU2013312198A1 (en) 2015-03-19
RU2679510C2 (ru) 2019-02-11
ZA201501736B (en) 2016-10-26
TW201424578A (zh) 2014-07-01
CN104736712A (zh) 2015-06-24
CA2883800A1 (en) 2014-03-13
JP2015527085A (ja) 2015-09-17
AR092482A1 (es) 2015-04-22
CA2883800C (en) 2021-07-13
EP2893024A1 (en) 2015-07-15
HK1210220A1 (en) 2016-04-15
WO2014039970A9 (en) 2014-10-09
BR112015004864B1 (pt) 2022-10-25
EP2893024A4 (en) 2016-03-30
IL237535A0 (en) 2015-04-30
WO2014039970A1 (en) 2014-03-13
IN2015DN01785A (cg-RX-API-DMAC7.html) 2015-05-29
KR20150052241A (ko) 2015-05-13
IL237535B (en) 2019-11-28
EP2893024B1 (en) 2019-12-04
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