CN111108929A - Method for making cistanche tubulosa-tamarisk integrated seedlings - Google Patents
Method for making cistanche tubulosa-tamarisk integrated seedlings Download PDFInfo
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- CN111108929A CN111108929A CN202010072569.4A CN202010072569A CN111108929A CN 111108929 A CN111108929 A CN 111108929A CN 202010072569 A CN202010072569 A CN 202010072569A CN 111108929 A CN111108929 A CN 111108929A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/029—Receptacles for seedlings
- A01G9/0293—Seed or shoot receptacles
Abstract
The invention discloses a method for manufacturing a cistanche tubulosa-Chinese tamarisk integrated seedling, which relates to the steps of sowing cistanche tubulosa seeds while cutting Chinese tamarisk branches, wherein the seed sowing is designed in a layered manner, the seeds are uniformly sown in a lower half layer of matrix, and the upper half part of the matrix has no seeds, so that the influence of the inoculation of the cistanche tubulosa seeds on the early development of the root system of the Chinese tamarisk can be avoided, and the over-concentrated distribution of the inoculation of the cistanche tubulosa can be effectively avoided; by accurately controlling the moisture in the seedling culture medium, the development of a root system and the survival of cutting branches are ensured, and the germination and inoculation of cistanche tubulosa seeds are promoted; the method has the characteristics of easy operation and easy popularization, is suitable for directly planting the Tamarix chinensis-cistanche tubulosa integrated seedlings in the field, is also suitable for the requirement of the public on planting cistanche tubulosa and bonsai on a balcony, and is beneficial to marketized popularization.
Description
The technical field is as follows:
the invention belongs to the technical field of crop cultivation, and particularly relates to a method for manufacturing cistanche tubulosa-tamarisk integrated seedlings.
Technical background:
cistanche, also known as golden bamboo shoot, herba speranskiae tuberculatae, commonly known as cistanches, is a perennial medicinal plant of cistanche genus of Orobanchaceae, has extremely high medicinal value, is worthy of the 'desert ginseng', and has the effects of tonifying kidney yang, benefiting essence and blood, relaxing bowel, improving immunity, protecting liver, inhibiting tumor and the like. The cistanche genus plants are divided into 4 varieties 1, namely: desert cistanche, fresh saline cistanche, white flower saline cistanche, desertliving cistanche and cistanche tubulosa.
Cistanche tubulosa is one of basic source plants of traditional Chinese medicine cistanche, is the most abundant wild resource and the most abundant seed with the largest yield in the cistanche plants at present, and is also the most easily developed large-scale cultivation seed in the cistanche plants. Besides, the cistanche tubulosa also has high ornamental value, the cistanche tubulosa is parasitized on the root system of the tamarisk specially, is inoculated underground and grows nutritionally, flowers and fruits on the ground, the inflorescence is conical, the inflorescence can reach 50 cm, the flower color is bright, and a string of white and purple flowers are bloomed. As a host plant of the cistanche tubulosa, the tamarix chinensis branches are flexible, the branches and the leaves are emerald green, the tamarix chinensis branches can be shaped, and then light purple florets are dotted, correspond to the out-of-soil cistanche tubulosa inflorescence, so that the cistanche tubulosa has good ornamental value, along with the deepening of the public understanding of the cistanche tubulosa, the demand of the cistanche tubulosa in the market is gradually increased, but the market supply is in short supply.
The key points of the potted seed-tube flower cistanche deserticola are that the proper soil water content is adjusted and the root system development of the tamarix chinensis is promoted. The current inoculation methods mainly comprise: the invention discloses a method for potting cistanche tubulosa (published with the number of CN 108370801A), which utilizes two substrates of two containers to create a condition suitable for the inoculation of the cistanche tubulosa and can quickly inoculate. Guo Yuhai et al invented a method of inoculating cistanche tubulosa with tamarisk stem (publication No. CN1864463A) and a method of inoculating cistanche tubulosa with light medium mesh bag container (publication No. CN 101772041A). The problems with these methods are: complex containers and materials are required, and the operation is complex; the specialty of inducing root system development is strong, needs professional operation and is difficult to popularize.
The invention content is as follows:
aiming at the defects of the prior art, the invention provides a method for preparing a cistanche tubulosa-tamarisk integrated seedling.
According to the method for manufacturing the cistanche tubulosa-tamarisk integrated seedling, provided by the invention, the development of a root system and the survival of a cutting branch are ensured by accurately controlling the moisture in a seedling culture medium, and the germination and inoculation of cistanche tubulosa seeds are promoted; the seeds of the cistanche tubulosa are sown while the tamarix chinensis branches are subjected to cuttage, the seeds are sown in a layered design, the seeds are uniformly sown in the lower half layer of matrix, and the upper half part of the matrix has no seeds, so that the influence of the inoculation of the cistanche tubulosa seeds on the early development of the root system of the tamarix chinensis can be avoided, and the over-concentrated distribution of the cistanche tubulosa inoculation can be effectively avoided; the inoculation number of the cistanche tubulosa is controlled by adjusting the seed quantity, the proper number can ensure the continuous growth of the cistanche tubulosa in the field in the later period, the cultivation of the Chinese tamarisk and the cistanche tubulosa seedlings by the seedling culture cup has the characteristics of easy operation and easy popularization, and the method is suitable for directly planting the Chinese tamarisk-cistanche tubulosa integrated seedlings in the field, is also suitable for the requirement of the public on planting the cistanche tubulosa and the bonsai on the balcony, and is beneficial to market popularization.
The technical scheme of the invention is as follows:
a method for making cistanche tubulosa-tamarisk integrated seedlings comprises the following steps:
(1) collecting cuttings: collecting non-germinated tamarisk branches from winter to 3 months of the next year, and cutting the branches into cuttings with the length of 18-22 cm.
(2) The cuttage method comprises the following steps: cutting before germination in winter to 4 months, cutting in a greenhouse in winter, controlling the temperature of the greenhouse to be 15-30 ℃, controlling the humidity to be 60-80%, wherein the seedling substrate can be purchased in the market, adding water into the seedling substrate and uniformly mixing, wherein the volume ratio of the water to the seedling substrate is 1 (8-10), mixing half of the seedling substrate added with the water into cistanche tubulosa seeds, the amount of the mixed seeds is 0.2-0.3g/L, adding half of the seedling substrate mixed with the seeds into a seedling container, then adding half of the seedling substrate not mixed with the seeds, dipping the base part 1/2 of the cutting slips into a rooting agent, and exposing the cutting slips for 2-3cm after cutting.
(3) Seedling stage management: controlling the water content of the seedling raising substrate after cuttage in the step (2), keeping the water content of the substrate at 70-90%, controlling the water content of the substrate until the newly-emerged buds grow to 18-20cm long, keeping the water content of the substrate at 20-40%, after cuttage for 10-12 weeks, keeping the water content of the substrate at 50-70% when the root system grows vigorously, performing the cuttage in a greenhouse in winter, controlling the temperature at 15-30 ℃ and the humidity at 60-80%, and enabling the cistanche tubulosa-tamarisk integrated seedlings to be on the market after cuttage planting for four months.
Preferably, in step (1), the tamarix chinensis is an adult plant.
Preferably, the branches collected in the step (1) are grown robust and disease and insect pest-free annual branches with the thickness of 1-1.5cm and smooth branches.
According to the invention, the cutting opening of the cutting slips obtained in the step (1) is flat at the top and inclined at the bottom, the cutting slips are graded in thickness and consistent in direction, and the cutting slips are buried in soil.
Further preferably, the branches are buried in moist soil sheltering from wind and shady, the buried depth of the branches is 3/4 times of the length of the cuttings, the branches are exposed 1/4, and watering and moisture preservation are carried out after the branches are buried in the soil.
According to the invention, the seedling raising substrate in the step (2) preferably comprises the following components in volume ratio: perlite: mixing vermiculite 1:1:1, and mixing uniformly.
According to the invention, the seedling raising container in the step (2) is preferably a black seedling raising cup.
More preferably, the seedling raising cup is 12cm in caliber width and 18cm in height, and the bottom of the seedling raising cup is provided with a hole.
Preferably, the rooting agent in the step (2) is viscous slurry containing 50mg/L of NAA and 25mg/L of IBA; preferably, in the step (2), the temperature is 18-25 ℃.
According to the invention, the water content is preferably controlled in the step (3) by absorbing water through the holes arranged at the bottom of the seedling cup; preferably, in the step (3), the temperature is 18-25 ℃.
The collection of the non-germinated tamarix chinensis branches is to prevent the germinated branches from influencing the survival rate of cuttage; inoculating seeds of the cistanche tubulosa while cutting the tamarix chinensis branches, uniformly sowing the seeds in the lower half layer of matrix, wherein the upper half part has no seeds, so that the development of the root system of the tamarix chinensis is not influenced; controlling the water content of the seedling raising substrate in the step (3) to be 70-90%, and promoting rooting and sprouting of the tamarix chinensis; controlling the water content at 20-40% to promote the root system of tamarix chinensis to grow; the water content is controlled to be 50-70%, and the germination and inoculation of cistanche tubulosa seeds are promoted; after the cistanche tubulosa-tamarisk integrated seedling is prepared, the diameter range of the cistanche tubulosa is 0.5-1cm, the average inoculation number is 10-15 per cup, and the cistanche tubulosa-tamarisk integrated seedling can be conveniently transplanted into a flowerpot or a field.
The invention has the beneficial effects that:
1. according to the method, the tamarix chinensis branches are cut in the seedling culture cup, the development of root systems and the survival of the cut branches are guaranteed through the accurate control of the moisture in the seedling culture medium, and the germination and inoculation of cistanche tubulosa seeds are promoted.
2. The seed of the cistanche tubulosa is inoculated while the tamarix chinensis branches are subjected to cuttage, the seed sowing is designed in a layered mode, the seeds are uniformly sown in the lower half layer of the matrix, the upper half part of the matrix is free of the seeds, the influence of inoculation of the cistanche tubulosa seeds on the root system development of the tamarix chinensis in the early stage can be avoided, and the over-concentrated distribution of the cistanche tubulosa inoculation can be effectively avoided.
3. The inoculation number of the cistanche tubulosa is controlled by adjusting the seed quantity, and the proper number can ensure the continuous growth of the cistanche tubulosa in the field at the later stage.
4. The method for manufacturing the cistanche tubulosa-tamarisk integrated seedling has the characteristics of easy operation and easy popularization, and has wide market prospect.
Description of the drawings:
FIG. 1: photo of the cistanche tubulosa-tamarisk integrated seedling cup prepared in example 1;
in the figure, a is an integrated seedling cup for 3 weeks of cuttage, and b is an integrated seedling cup for 7 weeks of cuttage;
FIG. 2 is a photograph of a cistanche tubulosa-Tamarix chinensis integrated seedling cultivated by layered inoculation in example 1 and non-layered inoculation in comparative example 1;
in the figure, c is the cistanche tubulosa-tamarisk integrated seedling cultivated by layered inoculation, and d is the cistanche tubulosa-tamarisk integrated seedling cultivated by non-layered inoculation.
The specific implementation mode is as follows:
the present invention is described in more detail below with reference to specific examples, but the scope of the present invention is not limited thereto.
Example 1
(1) Collecting cuttings: the method comprises the steps of collecting branches from adult Chinese tamarisk plants in 1 month, wherein the branches are required to be collected from annual branches without diseases and insect pests, the thickness is 1cm, the branches are smooth and have no branches, the branches are cut into 20cm, the cut openings are flat at the top and inclined at the bottom, the thickness is graded after collection, every 100 branches are bundled, and the directions of the branches are in accordance. Burying in moist soil with wind shielding and shady back, burying branch at 3/4 depth of the length of the cutting, exposing 1/4, watering, covering with grass, and keeping moisture.
(2) The cuttage method comprises the following steps: cutting in last ten days of 4 months, purchasing a seedling substrate in the market, wherein the seedling substrate comprises turfy soil, perlite and vermiculite (by volume) 1:1:1, adding water into the seedling substrate, uniformly mixing, and adding water: the volume ratio of the seedling substrate is 1:10, then half of the substrate is taken and mixed with the seeds, the amount of the seeds mixed in the substrate is 0.2g/L, the seedling substrate mixed with the seeds is filled into seedling cups, half of the seedling substrate is filled, the substrate without the seeds is filled, the seedling cups are compacted by hands, black plastic cups with the aperture width of 12cm and the height of 18cm are selected as the seedling cups, holes are formed in the bottoms of the black plastic cups for water absorption, branch bases 1/2 are dipped in viscous slurry containing 50mg/L NAA and 25mg/L IBA during cuttage, branches are promoted to take roots, the exposed parts of the branches are preferably 2cm, soil is compacted and placed in big white pots in order, 24 seedling cups can be placed in pots with the length, the width and the height of 61, 40.5 and 18cm, then the pots are irrigated, and the substrate absorbs enough water to promote the rooting of the branches.
(3) Seedling stage management: the growing environment in the seedling stage is a greenhouse, the temperature variation range is 15-30 ℃, the humidity is 65-70%, the greenhouse is moved outdoors when the temperature exceeds 30 ℃, the management in the seedling stage is mainly water control, more water is needed in the germination stage, water is poured 2 times per week in a manner of pouring water into a white basin, the substrate is ensured to absorb enough water, and the water content of the soil is kept at 70-90% at ordinary times; beginning to shoot buds 2 weeks after cuttage, beginning to control watering times and water quantity when newly-released buds about 5 weeks grow to be 20cm long, watering 1 time per week, keeping the water content of the soil at 20-40%, and promoting the growth of the root system of the tamarix chinensis; after 10 weeks of cuttage, the root system grows vigorously, and the soil is watered once per week, so that the soil humidity is kept at 50-70%, and the germination and inoculation of cistanche tubulosa seeds are promoted.
(4) The cutting planting is carried out for three and a half months, and the cistanche tubulosa-tamarisk integrated seedlings can be listed.
Comparative example 1
Compared with the example 1, the difference is that the layered inoculation is not carried out, the seed amount in the seedling substrate is 0.2g/L, the seedling substrate with the seed is filled in the seedling cup and compacted by hands, and the rest is the same.
Comparative example 2
Compared with the embodiment 1, the difference is that the seed amount mixed in the seedling substrate is 0.5g/L, the seedling substrate mixed with the seeds is filled into a seedling cup, half of the seedling substrate is filled, the substrate without the seeds is filled, the seedling substrate is compacted by hands, and the rest parts are the same.
Comparative example 3
Compared with the embodiment 1, the difference is that the water content of the substrate is controlled to be 50-70% all the time in the seedling management period and the whole growth period in the step (3), and the rest is the same.
Example 2
A method for making cistanche tubulosa-tamarisk integrated seedlings comprises the following steps:
(1) collecting cuttings: the method comprises the steps of collecting branches from adult Chinese tamarisk plants in 1 month, wherein the branches are required to be collected from annual branches without diseases and insect pests, the thickness is 1cm, the branches are smooth and have no branches, the branches are cut into 20cm, the cut openings are flat at the top and inclined at the bottom, the thickness is graded after collection, every 100 branches are bundled, and the directions of the branches are in accordance. Burying in moist soil with wind shielding and shady back, burying branch at 3/4 depth of the length of the cutting, exposing 1/4, watering, covering with grass, and keeping moisture.
(2) The cuttage method comprises the following steps: the seedling culture medium is purchased in the market, and the formula of the medium comprises turfy soil, perlite and vermiculite (by volume) 1:1: 1. Adding water into the matrix, and uniformly mixing, wherein the water: the substrate proportion is about 1:10 in volume ratio, then half of the substrate is taken and mixed with the seeds, the amount of the seeds mixed in the substrate is 0.3g/L, the seedling substrate mixed with the seeds is filled into a seedling cup, half of the seedling substrate is filled, the substrate without the seeds is filled, the seedling cup is compacted by hands, a black plastic cup with the aperture width of 12cm and the height of 18cm is selected from the seedling cup, a hole is formed in the bottom of the black plastic cup for water absorption, a branch base 1/2 is dipped in viscous slurry containing 50mg/L NAA and 25mg/L IBA during cuttage, the rooting of the branch is promoted, and the exposed part of the branch is preferably 2 cm. Compacting the soil, and neatly placing the soil in a big white basin. 24 seedling cups can be placed in pots with the length, width and height of 61, 40.5 and 18cm, then water is poured into the pots, and the substrate absorbs enough water to promote the rooting of the branches.
(3) Seedling stage management: the seedling stage growth is placed in a greenhouse, the temperature in the day/night in the greenhouse is 25/18 ℃, the humidity is 70%, the seedling stage management mainly comprises water control, more water is needed in the germination stage, water is poured 2 times a week in a manner of pouring water into a white pot, the substrate is ensured to absorb enough water, and the water content of the soil is kept at 70-90% in normal times. And (3) starting to shoot buds 2 weeks after cuttage, starting to control watering times and water quantity when new buds grow to 20cm long after 6 weeks, watering 1 time per week with the original water quantity of 1/3, keeping the water content of the soil at 20-40%, promoting the growth of the root system of the tamarix chinensis, flourishing the root system after 11 weeks of cuttage, watering once per week, keeping the humidity of the soil at 50-70%, and promoting the germination and inoculation of the cistanche tubulosa seeds.
(4) And planting for four months, and selling the cistanche tubulosa-tamarisk integrated seedlings on the market.
Examples of effects
The diameter range of the cistanche tubulosa prepared in the embodiments 1-2 and the comparative examples 1-3 is 0.5-1cm, and the inoculation amount is shown in table 1.
TABLE 1 quantity of cistanche tubulosa in different examples and comparative examples
| Numbering | Number of cistanche tubulosa inoculation/cup |
| Example 1 | 11.3 |
| Example 2 | 13.6 |
| Comparative example 1 | 29.2 |
| Comparative example 2 | 43.3 |
| Comparative example 3 | 6.7 |
And (4) analyzing results:
the layering inoculation is not carried out in the cutting seedling raising process of the tamarix chinensis branches, and the seeds are mixed into all the substrates in the seedling raising cups; or layered inoculation is carried out, but the inoculation amount is too large, the two inoculation modes can cause the number of the cistanche tubulosa in the finally cultivated integrated seedling to be too large, the distribution is concentrated, and the growth of the cistanche tubulosa in the later period is influenced; improper control of water in the cultivation process of the integrated seedlings can cause the number of the cistanche tubulosa in the finally cultivated integrated seedlings to be too small, and the practicability of the integrated seedlings is affected.
In the process of cutting seedling of the tamarix chinensis branches, the development of root systems and survival of the cutting branches are ensured by accurately controlling the moisture in the seedling culture medium, and the germination and inoculation of cistanche tubulosa seeds are promoted. The seed of the cistanche tubulosa is inoculated while the tamarix chinensis branches are subjected to cuttage, the seed sowing is designed in a layered mode, the seeds are uniformly sown in the lower half layer of the matrix, the upper half part of the matrix is free of the seeds, the influence of inoculation of the cistanche tubulosa seeds on the root system development of the tamarix chinensis in the early stage can be avoided, and the over-concentrated distribution of the cistanche tubulosa inoculation can be effectively avoided. The cistanche tubulosa-tamarisk integrated cup seedling obtained by the method has moderate inoculation rate of the cistanche tubulosa, and the inoculation quantity is 10-15 seeds/cup.
Claims (10)
1. A method for making cistanche tubulosa-tamarisk integrated seedlings is characterized by comprising the following steps:
(1) collecting cuttings: collecting non-germinated tamarix chinensis branches from winter to 3 months in the next year, and cutting the branches into cuttings with the length of 18-22 cm;
(2) the cuttage method comprises the following steps: cutting before germination in winter to 4 months, performing cutting in a greenhouse in winter, controlling the temperature to be 15-30 ℃, controlling the humidity to be 60-80%, wherein the seedling substrate can be purchased in the market, adding water into the seedling substrate and uniformly mixing, wherein the volume ratio of the water to the seedling substrate is 1 (8-10), mixing half of the seedling substrate added with the water into cistanche tubulosa seeds, the amount of the mixed seeds is 0.2-0.3g/L, adding half of the seedling substrate mixed with the seeds into a seedling container, then adding half of the seedling substrate not mixed with the seeds, dipping a cutting base 1/2 in a rooting agent, and exposing the cutting for 2-3cm after cutting;
(3) seedling stage management: controlling the water content of the seedling raising substrate after cuttage in the step (2), keeping the water content of the substrate at 70-90%, controlling the water content of the substrate until the newly-emerged buds grow to 18-20cm long, keeping the water content of the substrate at 20-40%, after cuttage for 10-12 weeks, keeping the water content of the substrate at 50-70% when the root system grows vigorously, performing the cuttage in a greenhouse in winter, controlling the temperature at 15-30 ℃ and the humidity at 60-80%, and enabling the cistanche tubulosa-tamarisk integrated seedlings to be on the market after cuttage planting for four months.
2. The method for making the cistanche tubulosa-tamarisk integrated seedling as claimed in claim 1, wherein the tamarix chinensis in step (1) is an adult plant.
3. The method for making cistanche tubulosa-tamarisk integrated seedlings according to claim 1, wherein the shoots collected in step (1) are robust, disease and insect pest free annual shoots with thickness of 1-1.5cm, smooth shoots and no branches.
4. The method of claim 3, wherein the cuttings of the step (1) are flat at the top and inclined at the bottom, and the cuttings are graded in thickness and uniform in direction and buried in soil.
5. The method for making cistanche tubulosa-tamarisk combined seedlings according to claim 4, wherein the cuttings obtained in step (1) are buried in moist soil protected from the wind and shady weather, the depth of the cuttings buried is 3/4 of the length of the cuttings, exposed 1/4 of the cuttings is exposed, and the cuttings are watered and moisturized after being buried in the soil.
6. The method for preparing cistanche tubulosa-tamarisk integrated seedlings according to claim 1, wherein the seedling substrate in the step (2) comprises the following components in volume ratio of turfy soil: perlite: mixing vermiculite 1:1:1, and mixing uniformly.
7. The method of claim 1, wherein the container for raising seedlings in step (2) is a black seedling cup.
8. The method of claim 7, wherein the seedling cup has a diameter of 12cm in width, a height of 18cm and a hole at the bottom.
9. The method of claim 1, wherein the rooting agent in step (2) is a viscous slurry containing 50mg/L NAA +25mg/L IBA; preferably, in the step (2), the temperature is 18-25 ℃.
10. The method of manufacturing cistanche tubulosa-tamarisk combined seedling as set forth in claim 1, wherein the water content is controlled in the step (3) by absorbing water through the holes formed at the bottom of the seedling cup to control the water content; preferably, in the step (3), the temperature is 18-25 ℃.
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