CN111000954A - Antibacterial gel and preparation method thereof - Google Patents
Antibacterial gel and preparation method thereof Download PDFInfo
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- CN111000954A CN111000954A CN201911398173.2A CN201911398173A CN111000954A CN 111000954 A CN111000954 A CN 111000954A CN 201911398173 A CN201911398173 A CN 201911398173A CN 111000954 A CN111000954 A CN 111000954A
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Abstract
The invention relates to the field of gynecological health care and nursing, and discloses an antibacterial gel which is characterized by comprising the following components in parts by weight: the raw materials comprise purified water, radix Stemonae, radix Sophorae Flavescentis, fructus Cnidii, cortex Phellodendri, Scutellariae radix, Carthami flos, Borneolum Syntheticum, lactobacillus, pear juice fermentation product filtrate, chlorhexidine acetate, carbomer, and triethanolamine. By adopting the technical scheme, the preparation method is simple, the preparation raw materials are easy to obtain, the preparation cost is low, good economic benefits are achieved, the prepared antibacterial gel is high in sterilization speed and sterilization efficiency, a large number of bacteria can be killed in a short time, no stimulation is generated on the application surfaces of vaginal mucosa and the like, the safety is high, the stability is good, and the recovery of patients is facilitated.
Description
Technical Field
The invention relates to the field of gynecological health care and nursing, in particular to an antibacterial gel and a preparation method thereof.
Background
The gynecological gel is a gynecological medicine, is mainly used for treating various gynecological vaginitis and cervicitis in the market at present, comprises bacterial vaginitis, trichomonas vaginitis, mixed vaginitis, gonococcus infection and chronic cervicitis, has the effects of diminishing inflammation, sterilizing and relieving itching, and has obvious inhibiting effect on the growth of pathogenic microorganisms such as staphylococcus aureus, escherichia coli, gonococcus, candida albicans, fungi, saccharomycetes and the like.
Disclosure of Invention
The invention provides an antibacterial gel and a preparation method thereof.
In order to solve the technical problems, the antibacterial gel adopts the technical scheme that raw materials comprise purified water, radix stemonae, radix sophorae flavescentis, fructus cnidii, cortex phellodendri, scutellaria baicalensis, safflower carthamus, borneol, lactobacillus, pear juice fermentation product filtrate, chlorhexidine acetate, carbomer and triethanolamine.
Further, the composition comprises the following components in parts by weight: 80-120 parts of purified water, 12-18 parts of radix stemonae, 20-26 parts of radix sophorae flavescentis, 20-26 parts of fructus cnidii, 7-9 parts of cortex phellodendri, 13-16 parts of scutellaria baicalensis, 4-7 parts of safflower, 6-9 parts of borneol, 3-5 parts of lactobacillus, 6-10 parts of pear juice fermentation product filtrate, 7-12 parts of chlorhexidine acetate, 10-20 parts of carbomer and 10-20 parts of triethanolamine.
Further, the composition comprises the following components in parts by weight: 100 parts of purified water, 15 parts of radix stemonae, 23 parts of radix sophorae flavescentis, 23 parts of fructus cnidii, 8 parts of cortex phellodendri, 15 parts of radix scutellariae, 6 parts of safflower, 8 parts of borneol, 4 parts of lactobacillus, 8 parts of pear juice fermentation product filtrate and 9 parts of chlorhexidine acetate.
Further, the preparation method of the bacteriostatic gel comprises the following steps:
s1, selecting radix stemonae, radix sophorae flavescentis, fructus cnidii, cortex phellodendri, scutellaria baicalensis and safflower according to parts by weight, cleaning after removing impurities, drying, respectively crushing, and sieving with a 60-100-mesh sieve for later use.
S2, adding 4-7 times of ethanol with the volume percentage of 70% -90% into the sophora flavescens processed in the step a, heating and refluxing for three times, wherein the ethanol is used for 1 hour each time, and filtering to obtain the sophora flavescens extract for later use.
S3, adding 6-8 times of ethanol with volume percentage of 60% -85% into the fructus cnidii processed in the step a, heating and refluxing for three times, wherein the ethanol is extracted for 45min each time, and filtering to obtain a radix sophorae flavescentis extract for later use.
S4, alkalifying the phellodendron amurense processed in the step a for 2-3 hours, then placing the phellodendron amurense into a supercritical extraction kettle for extraction for 3-4 hours to obtain a crude extract, adding 80-90% by volume of ethanol, precipitating for 10-12 hours, filtering a solution, recovering the ethanol to obtain a precipitate, dissolving the precipitate in hot methanol in a saturated manner, and standing and crystallizing for 3-5 times to obtain the phellodendron amurense extract for later use.
S5, adding 3-4 times of diethyl ether into the stemona processed in the step a, soaking for 30-40 min, taking supernatant, repeating the steps for three times, combining the supernatant, concentrating at low temperature, and concentrating to 1/20 times to obtain a stemona extract for later use.
S6, extracting the scutellaria baicalensis treated in the step a twice by using boiling water, adding 12-15 times of distilled water for the first time, and extracting for 1 hour; adding 8-10 times of distilled water for the second time, and extracting for 1.5 h; filtering with nonwoven fabric, mixing filtrates, and concentrating to 1/2 times to obtain Scutellariae radix extract.
S7, carrying out ultrasonic extraction on the safflower treated in the step a, wherein a solvent is 95% ethanol, the extraction time is 20-40 min, the extraction temperature is 40-60 ℃, and the extraction times are 2-3 times, so as to obtain a safflower extract for later use.
S8, taking borneol according to parts by weight, and adding a proper amount of ethanol for dissolving for later use.
S9, mixing the solutions obtained in the steps S2-S8, adding lactobacillus and pear juice fermentation product filtrate in parts by weight, adding purified water in proportion, uniformly mixing at normal temperature, adding carbomer, and stirring until the carbomer is completely dissolved for later use.
S10, adding chlorhexidine acetate into the solution obtained in the step S9 according to the weight part, uniformly stirring, dropwise adding triethanolamine according to the weight part, and stirring while dropwise adding until the gel state is uniform to obtain the antibacterial gel.
Furthermore, the antibacterial gel is applied to the fields of vagina cleaning, vagina bacteriostasis, vagina inflammation diminishing, vagina mucosa injury repairing, prevention and treatment and nursing after anorectal operation, inflammation diminishing and pain relieving.
By adopting the technical scheme, the preparation method is simple, the preparation raw materials are easy to obtain, the preparation cost is low, good economic benefits are achieved, the prepared antibacterial gel is high in sterilization speed and sterilization efficiency, a large number of bacteria can be killed in a short time, no stimulation is generated on the application surfaces of vaginal mucosa and the like, the safety is high, the stability is good, and the recovery of patients is facilitated.
Detailed Description
The following further describes the embodiments of the present invention. It should be noted that the description of the embodiments is provided to help understanding of the present invention, but the present invention is not limited thereto. In addition, the technical features involved in the embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other.
The first embodiment is as follows:
the bacteriostatic gel comprises the following components in parts by weight: 80 parts of purified water, 12 parts of radix stemonae, 20 parts of radix sophorae flavescentis, 20 parts of fructus cnidii, 7 parts of cortex phellodendri, 13 parts of radix scutellariae, 4 parts of safflower, 6 parts of borneol, 3 parts of lactobacillus, 6 parts of pear juice fermentation product filtrate, 7 parts of chlorhexidine acetate, 10 parts of carbomer and 10 parts of triethanolamine.
The preparation method of the bacteriostatic gel comprises the following steps:
s1, selecting radix stemonae, radix sophorae flavescentis, fructus cnidii, cortex phellodendri, scutellaria baicalensis and safflower according to parts by weight, removing impurities, cleaning, drying, respectively crushing, and sieving with a 60-mesh sieve for later use.
S2, adding 4 times of ethanol with the volume percentage of 70% into the sophora flavescens processed in the step a, heating and refluxing for three times, wherein the volume percentage of the ethanol is 1 hour each time, and filtering to obtain the sophora flavescens extract for later use.
And S3, adding 6 times of ethanol with volume percentage of 60% into the fructus cnidii processed in the step a, heating and refluxing for three times, wherein each time is 45min, and filtering to obtain a radix sophorae flavescentis extract for later use.
S4, alkalifying the phellodendron amurense processed in the step a for 2 hours, then placing the phellodendron amurense into a supercritical extraction kettle for extraction for 3 hours to obtain a crude extract, adding 80% ethanol by volume percentage, precipitating for 10 hours, filtering a solution, recovering the ethanol to obtain a precipitate, saturating and dissolving the precipitate with hot methanol, and standing and crystallizing for 3 times to obtain the phellodendron amurense extract for later use.
S5, adding 3 times of diethyl ether into the radix stemonae processed in the step a, soaking for 30min, taking supernate, repeating the steps for three times, combining the supernate, concentrating at low temperature, and concentrating to 1/20 times to obtain the radix stemonae extract for later use.
S6, extracting the scutellaria baicalensis treated in the step a twice by using boiling water, adding 12 times of distilled water by weight for the first time, and extracting for 1 hour; adding 8 times of distilled water for the second time, and extracting for 1.5 h; filtering with nonwoven fabric, mixing filtrates, and concentrating to 1/2 times to obtain Scutellariae radix extract.
S7, carrying out ultrasonic extraction on the safflower treated in the step a, wherein a solvent is 95% ethanol, the extraction time is 20min, the extraction temperature is 40 ℃, and the extraction times are 2 times, so as to obtain a safflower extract for later use.
S8, taking borneol according to parts by weight, and adding a proper amount of ethanol for dissolving for later use.
S9, mixing the solutions obtained in the steps S2-S8, adding lactobacillus and pear juice fermentation product filtrate in parts by weight, adding purified water in proportion, uniformly mixing at normal temperature, adding carbomer, and stirring until the carbomer is completely dissolved for later use.
S10, adding chlorhexidine acetate into the solution obtained in the step S9 according to the weight part, uniformly stirring, dropwise adding triethanolamine according to the weight part, and stirring while dropwise adding until the gel state is uniform to obtain the antibacterial gel.
An antibacterial gel is used in vagina cleaning, vagina bacteriostasis, vagina inflammation diminishing, vagina mucosa injury repairing, anorectal operation preventing and nursing, inflammation diminishing and pain relieving fields.
Example two:
the bacteriostatic gel comprises the following components in parts by weight: comprises the following components in percentage by weight: 100 parts of purified water, 15 parts of radix stemonae, 23 parts of radix sophorae flavescentis, 23 parts of fructus cnidii, 8 parts of cortex phellodendri, 15 parts of radix scutellariae, 6 parts of safflower, 8 parts of borneol, 4 parts of lactobacillus, 8 parts of pear juice fermentation product filtrate and 9 parts of chlorhexidine acetate.
The preparation method of the bacteriostatic gel comprises the following steps:
s1, selecting radix stemonae, radix sophorae flavescentis, fructus cnidii, cortex phellodendri, scutellaria baicalensis and safflower according to parts by weight, removing impurities, cleaning, drying, respectively crushing, and sieving with a 80-mesh sieve for later use.
S2, adding 5 times of ethanol with the volume percentage of 80% into the sophora flavescens processed in the step a, heating and refluxing for three times, wherein the volume percentage of the ethanol is 1 hour each time, and filtering to obtain the sophora flavescens extract for later use.
And S3, adding 7 times of ethanol with the volume percentage of 75% into the fructus cnidii processed in the step a, heating and refluxing for three times, wherein the ethanol is used for extracting for 45min each time, and filtering to obtain a radix sophorae flavescentis extract for later use.
S4, alkalifying the phellodendron amurense processed in the step a for 2.5 hours, then placing the phellodendron amurense into a supercritical extraction kettle for extraction for 3.5 hours to obtain a crude extract, adding 85% ethanol by volume percentage, precipitating for 11 hours, filtering a solution, recovering the ethanol to obtain a precipitate, saturating and dissolving the precipitate with hot methanol, and standing and crystallizing for 4 times to obtain the phellodendron amurense extract for later use.
S5, adding 3 times of diethyl ether into the radix stemonae processed in the step a, soaking for 35min, taking supernate, repeating the steps for three times, combining the supernate, concentrating at low temperature, and concentrating to 1/20 times to obtain the radix stemonae extract for later use.
S6, extracting the scutellaria baicalensis treated in the step a twice by using boiling water, adding 13 times of distilled water by weight for the first time, and extracting for 1 hour; adding 9 times of distilled water for the second time, and extracting for 1.5 h; filtering with nonwoven fabric, mixing filtrates, and concentrating to 1/2 times to obtain Scutellariae radix extract.
S7, carrying out ultrasonic extraction on the safflower treated in the step a, wherein a solvent is 95% ethanol, the extraction time is 30min, the extraction temperature is 50 ℃, and the extraction times are 2 times, so as to obtain a safflower extract for later use.
S8, taking borneol according to parts by weight, and adding a proper amount of ethanol for dissolving for later use.
S9, mixing the solutions obtained in the steps S2-S8, adding lactobacillus and pear juice fermentation product filtrate in parts by weight, adding purified water in proportion, uniformly mixing at normal temperature, adding carbomer, and stirring until the carbomer is completely dissolved for later use.
S10, adding chlorhexidine acetate into the solution obtained in the step S9 according to the weight part, uniformly stirring, dropwise adding triethanolamine according to the weight part, and stirring while dropwise adding until the gel state is uniform to obtain the antibacterial gel.
An antibacterial gel is used in vagina cleaning, vagina bacteriostasis, vagina inflammation diminishing, vagina mucosa injury repairing, anorectal operation preventing and nursing, inflammation diminishing and pain relieving fields.
Example three:
the bacteriostatic gel comprises the following components in parts by weight: 120 parts of purified water, 18 parts of radix stemonae, 26 parts of radix sophorae flavescentis, 26 parts of fructus cnidii, 9 parts of cortex phellodendri, 16 parts of radix scutellariae, 7 parts of safflower, 9 parts of borneol, 5 parts of lactobacillus, 10 parts of pear juice fermentation product filtrate, 12 parts of chlorhexidine acetate, 20 parts of carbomer and 20 parts of triethanolamine.
The preparation method of the bacteriostatic gel comprises the following steps:
s1, selecting radix stemonae, radix sophorae flavescentis, fructus cnidii, cortex phellodendri, scutellaria baicalensis and safflower according to parts by weight, cleaning after removing impurities, drying, respectively crushing, and sieving with a 100-mesh sieve for later use.
S2, adding 7 times of ethanol with the volume percentage of 90% into the sophora flavescens processed in the step a, heating and refluxing for three times, wherein the volume percentage of the ethanol is 1 hour each time, and filtering to obtain the sophora flavescens extract for later use.
And S3, adding 8 times of 85% ethanol by volume percentage into the fructus cnidii processed in the step a, heating and refluxing for three times, wherein each time is 45min, and filtering to obtain a radix sophorae flavescentis extract for later use.
S4, alkalifying the phellodendron amurense processed in the step a for 3 hours, then placing the phellodendron amurense into a supercritical extraction kettle for extraction for 4 hours to obtain a crude extract, adding 90% ethanol by volume percentage, precipitating for 12 hours, filtering a solution, recovering the ethanol to obtain a precipitate, saturating and dissolving the precipitate with hot methanol, and standing and crystallizing for 5 times to obtain the phellodendron amurense extract for later use.
S5, adding 4 times of diethyl ether into the radix stemonae processed in the step a, soaking for 40min, taking supernate, repeating the steps for three times, combining the supernate, concentrating at low temperature, and concentrating to 1/20 times to obtain the radix stemonae extract for later use.
S6, extracting the scutellaria baicalensis treated in the step a twice by using boiling water, adding 15 times of distilled water by weight for the first time, and extracting for 1 hour; adding 10 times of distilled water for the second time, and extracting for 1.5 h; filtering with nonwoven fabric, mixing filtrates, and concentrating to 1/2 times to obtain Scutellariae radix extract.
S7, carrying out ultrasonic extraction on the safflower treated in the step a, wherein a solvent is 95% ethanol, the extraction time is 40min, the extraction temperature is 60 ℃, and the extraction times are 3 times, so as to obtain a safflower extract for later use.
S8, taking borneol according to parts by weight, and adding a proper amount of ethanol for dissolving for later use.
S9, mixing the solutions obtained in the steps S2-S8, adding lactobacillus and pear juice fermentation product filtrate in parts by weight, adding purified water in proportion, uniformly mixing at normal temperature, adding carbomer, and stirring until the carbomer is completely dissolved for later use.
S10, adding chlorhexidine acetate into the solution obtained in the step S9 according to the weight part, uniformly stirring, dropwise adding triethanolamine according to the weight part, and stirring while dropwise adding until the gel state is uniform to obtain the antibacterial gel.
An antibacterial gel is used in vagina cleaning, vagina bacteriostasis, vagina inflammation diminishing, vagina mucosa injury repairing, anorectal operation preventing and nursing, inflammation diminishing and pain relieving fields.
Wherein, the scutellaria baicalensis extract: baicalin is easily soluble in N, N-dimethylformamide and pyridine, can be dissolved in alkali liquids such as sodium bicarbonate, sodium carbonate and sodium hydroxide, and is unstable in the alkali liquids and gradually turns dark brown; slightly soluble in hot glacial acetic acid and insoluble in methanol, ethanol and acetone; hardly soluble in water, ether, benzene, chloroform, etc.
The first table shows the extraction conditions of baicalin in different solvents
| Kind of solvent | Baicalin content |
| Water extraction | 0.1292 |
| 70% ethanol | 0.06452 |
| 95% ethanol | 0.09318 |
As shown in Table I, baicalin is extracted with water as solvent.
The invention is not limited to the described embodiments. It will be apparent to those skilled in the art that various changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, and the scope of protection is still within the scope of the invention.
Claims (5)
1. An antibacterial gel, which is characterized in that: the raw materials comprise purified water, radix Stemonae, radix Sophorae Flavescentis, fructus Cnidii, cortex Phellodendri, Scutellariae radix, Carthami flos, Borneolum Syntheticum, lactobacillus, pear juice fermentation product filtrate, chlorhexidine acetate, carbomer, and triethanolamine.
2. A bacteriostatic gel according to claim 1, wherein: comprises the following components in percentage by weight: 80-120 parts of purified water, 12-18 parts of radix stemonae, 20-26 parts of radix sophorae flavescentis, 20-26 parts of fructus cnidii, 7-9 parts of cortex phellodendri, 13-16 parts of scutellaria baicalensis, 4-7 parts of safflower, 6-9 parts of borneol, 3-5 parts of lactobacillus, 6-10 parts of pear juice fermentation product filtrate, 7-12 parts of chlorhexidine acetate, 10-20 parts of carbomer and 10-20 parts of triethanolamine.
3. A bacteriostatic gel according to claim 2, wherein: comprises the following components in percentage by weight: 100 parts of purified water, 15 parts of radix stemonae, 23 parts of radix sophorae flavescentis, 23 parts of fructus cnidii, 8 parts of cortex phellodendri, 15 parts of radix scutellariae, 6 parts of safflower, 8 parts of borneol, 4 parts of lactobacillus, 8 parts of pear juice fermentation product filtrate and 9 parts of chlorhexidine acetate.
4. A bacteriostatic gel according to any one of claims 1-3, wherein: the preparation method of the bacteriostatic gel comprises the following steps:
s1, selecting radix stemonae, radix sophorae flavescentis, fructus cnidii, cortex phellodendri, scutellaria baicalensis and safflower according to parts by weight, cleaning after removing impurities, drying, respectively crushing, and sieving with a 60-100-mesh sieve for later use;
s2, adding 4-7 times of ethanol with the volume percentage of 70% -90% by weight into the sophora flavescens processed in the step a, heating, refluxing and extracting for three times, wherein the time is 1 hour each time, and filtering to obtain a sophora flavescens extract for later use;
s3, adding 6-8 times of ethanol with volume percentage of 60% -85% into the fructus cnidii processed in the step a, heating and refluxing for three times, wherein the ethanol is used for extracting for 45min each time, and filtering to obtain a radix sophorae flavescentis extract for later use;
s4, alkalifying the phellodendron amurense processed in the step a for 2-3 hours, then placing the phellodendron amurense into a supercritical extraction kettle for extraction for 3-4 hours to obtain a crude extract, adding 80-90% ethanol by volume percentage, precipitating for 10-12 hours, filtering a solution, recovering the ethanol to obtain a precipitate, dissolving the precipitate in hot methanol in a saturated manner, standing and crystallizing for 3-5 times to obtain the phellodendron amurense extract for later use;
s5, adding 3-4 times of diethyl ether into the radix stemonae processed in the step a, soaking for 30-40 min, taking supernate, repeating for three times, combining the supernate, concentrating at low temperature, and concentrating to 1/20 times to obtain a radix stemonae extract for later use;
s6, extracting the scutellaria baicalensis treated in the step a twice by using boiling water, adding 12-15 times of distilled water for the first time, and extracting for 1 hour; adding 8-10 times of distilled water for the second time, and extracting for 1.5 h; filtering with nonwoven fabric, mixing filtrates, and concentrating to 1/2 times to obtain Scutellariae radix extract;
s7, carrying out ultrasonic extraction on the safflower treated in the step a, wherein a solvent is 95% ethanol, the extraction time is 20-40 min, the extraction temperature is 40-60 ℃, and the extraction times are 2-3 times, so as to obtain a safflower extract for later use;
s8, taking borneol according to parts by weight, and adding a proper amount of ethanol for dissolving for later use;
s9, mixing the solutions obtained in the steps S2-S8, adding lactobacillus and pear juice fermentation product filtrate in parts by weight, adding purified water in proportion, uniformly mixing at normal temperature, adding carbomer, and stirring until the carbomer is completely dissolved for later use;
s10, adding chlorhexidine acetate into the solution obtained in the step S9 according to the weight part, uniformly stirring, dropwise adding triethanolamine according to the weight part, and stirring while dropwise adding until the gel state is uniform to obtain the antibacterial gel.
5. Use of a bacteriostatic gel according to any one of claims 1-4 in the fields of vaginal cleaning, vaginal bacteriostasis, vaginal inflammation diminishing, repair of vaginal mucosal lesions, prevention and treatment after anorectal surgery, and anti-inflammatory pain relieving.
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| CN112245495A (en) * | 2020-11-21 | 2021-01-22 | 陕西远志医药生物工程有限公司 | Slow-release antibacterial gel and preparation method thereof |
| CN112516209A (en) * | 2020-12-28 | 2021-03-19 | 陕西兴曜生物科技有限公司 | Bacteriostatic gel and preparation method thereof |
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